How can I creating multisample VCF file ?

How can I creating multisample VCF file ?

0

Hello,
I want to create a multisample VCF file. I have bam files from various alignment.

I ran the command

bcftools mpileup -d 100000 -f ~reference *.bam | bcftools call -c > concate.vcf

Is this step scientifically correct? Or do I need to

merge all bam first before running the above command?

Thanks


Bioinformatics

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