Solved CHM/BIO 4361 Protein Concentration Lab Additional

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CHM/BIO 4361 Protein Concentration Lab Additional Questions 1.) Using the primary sequences provided, compare the molecular weights and relative Trp and Tyr content percentages of our two proteins to the extinction coefficients given by Expasy ProtParam (assuming fully formed disulfides for all). Because ProtParam depends only on primary sequence, you can paste all chains into the tool in any order to obtain an accurate result. According to the absorbance spectra shown in the textbook/lecture, Trp absorbs much more strongly than Tyr. Examine the protein sequences: Does the extinction coefficient always increase when Trp percentage increases? Why or why not? 2.) If you used the computer screen method to measure the results of the Bradford assay, what RGB color value should you use for the background?

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