Between sample normalization without replicates

Between sample normalization without replicates

0

Hi All,

I have some RNA-Seq data without reps for 5 different conditions. I can not do any fancy statistics with this data. Just trying to get the idea if the genes of interest show stable expression in these conditions. What is the best way to normalize counts between samples?

I tried generating TPMs using salmon quant but it seems to do only within-sample normalization or multiple reps/libs concatenated together. Any suggestions for tools and methods for this?

Thanks!


normalization


RNA-Seq

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