Investigation of PIT1 gene polymorphisms and their influence on milk traits in dairy cattle

Type of publication:  conference paper Type of publication (PDB):  Tezės kitose duomenų bazėse / Theses in other databases (T1c) Field of Science:  Biologija / Biology (N010);Gyvūnų mokslai / Animal sciences (A003) Author(s):  Bižienė, Renata;Morkūnienė, Kristina;Kolaitytė, Gintarė;Mišeikienė, Ramutė;Pečiulaitienė, Nijolė;Kučinskas, Laimutis Title:  Investigation of PIT1 gene polymorphisms and their influence on milk traits in dairy cattle Is part of:  Veterinarija ir zootechnika : 2nd international scientific conference “Livestock production: recent trends and future prospects” : 2022 November 16. Kaunas / Lithuanian University of Health Sciences. Veterinary Academy. Faculty of Animal Sciences : abstracts. Kaunas : Lietuvos sveikatos mokslų universiteto Veterinarijos akademija., 2022, vol. 80, suppl. 2 Extent:  p. 90-90 Date:  2022 Note:  Bibliogr.: p. 90 Keywords:  dairy cattle;PIT1 gene;RFLP Abstract:  Investigation of gene polymorphisms is very useful in determining the genetic potential of an animal [1]. The use of genomic or marker selected analysis in cattle breeding can effectively improve the quality of dairy products and better results of quantitative traits [2]. Previous studies in dairy cattle have shown that polymorphisms in the pituitrin-specific transcription factor (PIT1) gene are associated with traits of cattle milk productivity. The pituitrin-specific transcription factor (PIT1 or POU1F1) gene is involved in the transcription of the growth hormone (GH) gene, the prolactin (PRL) gene, and the thyroid stimulating hormone-β (TSHB) gene. Pituitrin-specific transcription factor is also important for the differentiation and growth of adenohypophyseal somatotropic, lactotropic, and thyrotropic cells, pituitary development, and maintenance of function [3]. Blood samples were taken from 101 dairy cattle from various dairy farms in Lithuania. Data sets for second lactation production period from dairy cattle were analysed. In this study, two PIT1 gene polymorphisms (c.1178A/G and c.545G/A) were analysed. Genotyping of polymorphisms was achieved using the PCR-RFLP method. There were three genotypes detected at PIT1 gene polymorphism c.1178A/G, i.e., BB, AB and AA. The observed frequencies were 64.4% (BB), 27.7% (AB) and 7.9% (AA). The observed allele frequencies were B 78.2% and A 21.8%, respectively. In this polymorphism, heterozygosity was insufficient. There were three genotypes detected at PIT1 gene polymorphism c.545G/A, i.e., GG, GA and AA. Their observed frequencies were GG 62.4%, GA 27.7% and AA 9.9%. The mutated allele A frequency was 23.8% and the wild type allele G frequency was 78.2%. In this polymorphism, heterozygosity was also insufficient. The investigational analysis of PIT1 gene polymorphism c.1178A/G shows that B allele determines higher milk yield, while PIT1 gene polymorphism c.545G/A allele A determines higher milk and protein yield. In conclusion, results from the analysis show that PIT1 gene polymorphisms c.1178A/G and c.545G/A have a significant impact on the quantity of milk and protein yield Internet:  hdl.handle.net/20.500.12512/117028 Affiliation(s):  Biologinių sistemų ir genetinių tyrimų institutas
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Lietuvos sveikatos mokslų universitetas Appears in Collections: Universiteto mokslo publikacijos / University Research Publications

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