A. What type of FACS data graphical representations

Transcribed image text:

A. What type of FACS data graphical representations are Panel A and Panel B? (1 Mark) B. All data displayed in Panel B have been gated from data displayed in Panel A using a FSC and SSC gate. What does this statement mean? Why would you want to do this? (1 Mark) C. Which of the shRNA vectors targeted to Scribble ( 1 or 2 ) is most effective in knocking down Scribble? Is it as effective as the shRNA targeting GFP? Why might this be the case? ( 1 Mark) D. Does loss of expression of Scribble in the above experiment alter cell shape or cell size? Explain your rationale for the answer. ( 1 Mark) shGFP alone and the shScrib1 transfected cells in terms of GFP intensity? What chemical stain might you use to identify the nucleus of each cell? How does it work? (1 Mark)

Read more here: Source link