Is it possible to do DGE analysis using log 2 normalized data with EdgeR ?
Hello everyone,
I intend to analyze differential gene expression using a GEO dataset. The value is log2 normalized signal intensity.
I know edgeR’s workflow involves log normalization. However, can I skip the normalization steps and continue the rest of the analysis (estimate the BCV(s) and make pairwise comparisons)? Or do I need to analyze from scratch (raw data)?
I have read the user’s guide and searched online, but I did not find an answer to my question.
Thank you very much for any help you can provide. Have a nice day!
• 42 views
Read more here: Source link