doi: 10.1093/bioinformatics/btad150.
Online ahead of print.
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Bioinformatics.
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Abstract
Motivation:
Iso-Seq RNA long read sequencing enables the identification of full-length transcripts and isoforms, removing the need for complex analysis such as transcriptome assembly. However, the raw sequencing data need to be processed in a series of steps before annotation is complete. Here, we present nf-core/isoseq, a pipeline for automatic read processing and genome annotation. Following nf-core guidelines, the pipeline has few dependencies and can be run on any of platforms.
Availability:
The pipeline is freely available online on the nf-core website (nf-co.re/isoseq) and on github (github.com/nf-core/isoseq) under MIT License (DOI: 10.5281/zenodo.7116979).
Supplementary information:
Supplementary data are available at Bioinformatics online.
© The Author(s) 2023. Published by Oxford University Press.
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