GFP Stably expressing CHO Cell Line

The CHO cell line is an adherent epithelial cell derived from the ovary of Chinese hamster. It is widely used in biology and medical research of genetics, toxicity screening, nutrition, and gene expression, particularly to express recombinant proteins. CHO cells are the most common mammalian hosts for industrial production of recombinant protein therapeutics. CHO-GFP stable cell line is transformed from the CHO cell line and stably expresses the GFP fluorescent protein. Both GFP and geneticin-resistant genes are introduced into parental CHO cells using retrovirus. Strong Green fluorescent signal can be visualized in every single cell under fluorescent microscope. The expression of GFP has been validated by western blot.

Fluorescence microscope images.
Fluorescence microscope images.
Western blot analysis and Auto-western analysis.

Western blot analysis and Auto-western analysis.

Lane M: Protein marker.
1: CHO
Rabbit anti-GFP: 130-11011 (1:1000)

  • Minimum Essential Medium α (MEM α)
  • Fetal Bovine Serum (FBS)
  • Penicillin/Streptomycin
  • Trypsin
  • Phosphate-buffered saline (PBS)
  • DMSO
  • 96-well white plate

Store the frozen cells in liquid nitrogen until you are ready to thaw and propagate them. It is strongly recommended that you propagate the cells following instructions as soon as possible upon arrival. An adequate number of frozen stocks must be made from early passages as cells will undergo genotypic changes. Genetic instability in transfected cells will result in a decreased responsiveness over time in normal cell culture conditions.

Read more here: Source link