Pax3 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details


Assay

Reference:


J:45678

Henderson DJ, et al., Over-expression of the chondroitin sulphate proteoglycan versican is associated with defective neural crest migration in the Pax3 mutant mouse (splotch). Mech Dev. 1997 Dec;69(1-2):39-51

Assay type:

RNA in situ

MGI Accession ID:

MGI:5811768

Gene symbol:

Pax3

Gene name:

paired box 3


Probe:


Pax3 probe39


Probe preparation:

Antisense labelled with digoxigenin RNA

Visualized with:

Alkaline phosphatase
Results


Specimen

E9.0 wt not shown:

embryonic day 9.0

(more )

Structure Level Pattern Image Note
TS14: somite Weak Regionally restricted

Low levels of expression in somitic mesoderm.



Specimen

E9.5 wt not shown:

embryonic day 9.5

(more )

Structure Level Pattern Image Note
TS15: dermomyotome Present

Expression restricted to dermomyotome.

TS15: sclerotome Absent

Expression is down-regulated in the sclerotome.



Specimen

6A:

embryonic day 10.5

(more )


Note: Specimens were embedded in a mixture of 0.45% gelatine, 27% albumen, 18% glucose in PBS, solidified by addition of 1/10 volume of 30% glutaraldehyde.



Specimen

6C:

embryonic day 10.5; Pax3Sp-2H/Pax3Sp-2H

(more )


Note: Specimens were embedded in a mixture of 0.45% gelatine, 27% albumen, 18% glucose in PBS, solidified by addition of 1/10 volume of 30% glutaraldehyde.

Structure Level Pattern Image Note
TS17: neural tube Present Regionally restricted


6C

Authors state that truncated transcripts present in similar distribution to wild type in dorsal neural tube.

TS17: dermomyotome Weak


6C

Expression diminished in dermomyotome.

TS17: limb bud Absent


6C



Specimen

7A:

embryonic day 11.5

(more )


Note: Specimens were embedded in a mixture of 0.45% gelatine, 27% albumen, 18% glucose in PBS, solidified by addition of 1/10 volume of 30% glutaraldehyde.



Specimen

7C:

embryonic day 11.5; Pax3Sp-2H/Pax3Sp-2H

(more )


Note: Specimens were embedded in a mixture of 0.45% gelatine, 27% albumen, 18% glucose in PBS, solidified by addition of 1/10 volume of 30% glutaraldehyde.


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