KEGG ENZYME: 1.14.14.10

Entry
EC 1.14.14.10               Enzyme                                 
Name

nitrilotriacetate monooxygenase

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

nitrilotriacetate,FMNH2:oxygen oxidoreductase (glyoxylate-forming)

Reaction(IUBMB)
nitrilotriacetate + FMNH2 + H+ + O2 = iminodiacetate + glyoxylate + FMN + H2O [RN:R09798]
Reaction(KEGG) Substrate Product Comment
Requires Mg2+. The enzyme from Aminobacter aminovorans (previously Chelatobacter heintzii) is part of a two component system that also includes EC 1.5.1.42 (FMN reductase), which provides reduced flavin mononucleotide for this enzyme.
History

EC 1.14.14.10 created 2011

Orthology
K20147   nitrilotriacetate monooxygenase
Genes Reference   Authors

Uetz T, Schneider R, Snozzi M, Egli T

  Title

Purification and characterization of a two-component monooxygenase that hydroxylates nitrilotriacetate from “Chelatobacter” strain ATCC 29600.

  Journal   Sequence Reference   Authors

Knobel HR, Egli T, van der Meer JR

  Title

Cloning and characterization of the genes encoding nitrilotriacetate monooxygenase of Chelatobacter heintzii ATCC 29600.

  Journal   Sequence Reference   Authors

Xu Y, Mortimer MW, Fisher TS, Kahn ML, Brockman FJ, Xun L

  Title

Cloning, sequencing, and analysis of a gene cluster from Chelatobacter heintzii ATCC 29600 encoding nitrilotriacetate monooxygenase and NADH:flavin mononucleotide oxidoreductase.

  Journal Other DBs
ExPASy – ENZYME nomenclature database:  1.14.14.10
UM-BBD (Biocatalysis/Biodegradation Database):  1.14.14.10

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