BCL2 fusions not detected in RNA level

BCL2 fusions not detected in RNA level

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Hi all,

I’ve designed ~120bp probe panel to capture the target sequences, including BCL2, BCL6, MYC genes.

In sequencing data, we found some BCL2+ samples, which had been tested with FISH, were not detected in RNA level.

Both StarFusion and FusionCatcher were applied to detect the fusions.

It was reported that regions called MBR, MCR, icr, were previously discovered as hot breaking regions. Since regions like MCR, icr are away from gene UTR regions , we did not set the probes to cover these regions.

I am confused that why these fusions were not found in RNA levels, or these fusions can only be detectable in DNA level?

Best,
Junfeng


fusion


RNA


BCL2

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