GC content of WES cohort

GC content of WES cohort

0

Hello,

I know a lot of questions have been asked before about GC content, but I just wanted to see if anyone had experience with WES GC content. I have 134 WES (not all the same exome panel or from the same sequencing company).

From reading the FASTQC manual I can see that if it is more than 15% it may be caused by bias?

Does anyone have any experience of this, and if so does my data look okay? This was made on FASTQC and then combined using multiqc.
I will attach a before and after from cutadapt.

Before trimming:

enter image description here

After trimming:

enter image description here

Thanks!


WES


FASTQC


GC-content

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