Solved 1.1. If the target sequence or restriction site is

1.1. If the target sequence or restriction site is 5′-GAATTC-3′, what is the complementary sequence read in the 3′−5 ‘ direction. Give a reason for your answer. 1.2. miRNA-mediated gene silencing represents a back-up pathway should siRNA-mediated gene silencing be unsuccessful at silencing the target dsRNA. Is this statement true or false? Explain. 1.3. You discover that the guide RNA for a CRISPR experiment has 21 off-target sites. 1.2.1. If the human genome contains 3.2 billion base pairs. What is the approximate frequency of off-target effects? 1.2.2. Do you think this is a tolerable error rate? Explain.