Create customized gene annotation file

Create customized gene annotation file

0

Hi,

I have self-defined RNA sequence, it was used to tag the special cells I’m interested in. I want to use cellranger mkref to build a custom reference and use this reference file to count the cells that have this sequence. Do you have any ideas on how to generate gtf/fasta files if I only have an RNA sequence?

Thanks!!!


reference


mkref


genome


cellranger

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