Find potential important genes from bulk-RNA seq experiment

Find potential important genes from bulk-RNA seq experiment

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Hi Biostars,

After finding DEG genes, I try to find important genes that may cause for a disease so I do pathway analysis with GSEA and find pathway with highest enrichment score using gseKEGG() and gseGO(). Is that genes in the pathway with highest enrichment score is the one I should focus on? Or other analysis I should perform from bulk RNA-seq data to find the important gene? I appreciate your help!


RNA-seq

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