Tag: ACE2

Dimitrov, D. S. Virus entry: molecular mechanisms and biomedical applications. Nat. Rev. Microbiol. 2004 22 2, 109–122 (2004). Google Scholar  Chen, W., Zarnitsyna, V. I., Sarangapani, K. K., Huang, J. & Zhu, C. Measuring Receptor-Ligand Binding Kinetics on Cell Surfaces: From Adhesion Frequency to Thermal Fluctuation Methods. Cell. Mol. Bioeng….

WHO Coronavirus (COVID-19) Dashboard | WHO Coronavirus (COVID-19) Dashboard With Vaccination Data. 2023/10/18. covid19.who.int/. Accessed 18 Oct 2023. Zhai P, Ding Y, Wu X, Long J, Zhong Y, Li Y. The epidemiology, diagnosis and treatment of COVID-19. Int J Antimicrob Agents. 2020;55:105955. doi.org/10.1016/j.ijantimicag.2020.105955. Article  CAS  PubMed  PubMed Central  Google Scholar …

Plasmid construction All the constructs used in this study were generated with standard cloning strategies, including PCR, overlapping PCR, oligo annealing, digestion and ligation. Primers were purchased from Genewiz. The plasmid sequence was verified by Sanger sequencing. The pCAG-spike(D614G)-GFP11-mCherry plasmid was modified from Addgene plasmid 158761. Briefly, GFP11 and mCherry…

In this study, we sequenced and characterized 49 SARS-CoV-2 samples from Uganda, collected between April 2020 and July 2021, using MinION Nanopore sequencing. Overall, the ARTIC protocol used was able to generate the required libraries for successful sequencing on the long-read sequencer. MinION Oxford Nanopore technology enabled the identification of…

Cele S, Gazy I, Jackson L, Hwa SH, Tegally H, Lustig G, et al. Escape of SARS-CoV-2 501Y.V2 from neutralization by convalescent plasma. Nature. 2021;593(7857):142–6. Article  CAS  PubMed  PubMed Central  Google Scholar  Planas D, Bruel T, Grzelak L, Guivel-Benhassine F, Staropoli I, Porrot F, et al. Sensitivity of infectious SARS-CoV-2…

Mice and Golden Syrian hamsters All research experiments in this study comply with all relevant ethical regulations. The Institutional Animal Care and Use Committee approved the animal protocol at the University of Maryland (#R-APR-20-18 and #R-MAR-21-19). The animals were acclimatized at the animal facility for 4–6 days before initiating experiments….

Kanoktip Petcharat,1 Narongsuk Munkong,2 Rungthip Thongboontho,1 Widsanusan Chartarrayawadee,3 Arthid Thim-Uam1 1Division of Biochemistry, School of Medical Sciences, University of Phayao, Phayao, 56000, Thailand; 2Department of Pathology, School of Medicine, University of Phayao, Phayao, 56000, Thailand; 3Division of Chemistry, School of Science, University of Phayao, Phayao, 56000, Thailand Correspondence: Arthid Thim-Uam,…

BAC plasmids Human (CH17-203N23, CH17-449P15 and CH17-339H2) and mouse (RP23-51O13, RP23-75P20 and RP23-204E8) BACs were purchased from BACPAC Resources Center. Yeast–bacterium shuttle vector pLM1050 was modified by L. Mitchell based on a previous study28. pWZ699 was constructed by inserting a cassette containing pPGK-ΔTK-SV40pA transcription unit and the Actb gene into…

Cells and viruses Haploid mSCs AN3–12 are a feeder independent clonal derivative of HMSc2 isolated from mice oocyte and maintained at IMBA52. The AN3–12 library and knocked out cells used for the haploid screening was obtained from IMBA (Austria)14,52. AN3–12 cells were validated by STR analysis. Haploid mES cells were…

Dong, E., Du, H. & Gardner, L. An interactive web-based dashboard to track COVID-19 in real time. Lancet Infect. Dis. 20, 533–534 (2020). Article  CAS  PubMed  PubMed Central  Google Scholar  Tan, W. et al. A novel coronavirus genome identified in a cluster of pneumonia cases – Wuhan, China 2019–2020. China…

Introduction Coronavirus disease 2019 (COVID-19) first broke out in Wuhan, China, in December 2019. Subsequently, COVID-19 spread rapidly and widely, causing a pandemic.1 It has emerged as a well-known human pandemic over the last three years due to its high pathogenicity and infectivity, which posed serious risks to public health.2…

World Health Organization. WHO Coronavirus (COVID-19) Dashboard. Available at covid19.who.int/ (2023). de Vries, M. et al. A comparative analysis of SARS-CoV-2 antivirals characterizes 3CLpro inhibitor PF-00835231 as a potential new treatment for COVID-19. J. Virol. 95, e01819-20 (2021). Article  PubMed  PubMed Central  Google Scholar  FDA. Emergency Use Authorization (Eua) of…

Lytras, S., Xia, W., Hughes, J., Jiang, X. W. & Robertson, D. L. The animal origin of SARS-CoV-2. Science 373, 968–970 (2021). Article  ADS  CAS  PubMed  Google Scholar  Ye, Z. W. et al. Zoonotic origins of human coronaviruses. Int J Biol Sci 16, 1686–1697 (2020). Article  CAS  PubMed  PubMed Central …

Introduction The coronavirus infectious diseases-2019 worldwide pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is currently ongoing after being emerged from Wuhan (China) in December 2019 (Ecdc, 2020; Zhu et al, 2020; Spiteri et al, 2020). Up to now, SARS-CoV-2 has infected more than 750 million people and…

Baseline characteristics of study participants A total of 40 pregnant women were enrolled into the study, with 13 in the convalescent group and 27 in the control group. During COVID-19 infection in the 13 convalescent women, one had a temporary loss of taste, one had occasional palpitations, and three had…

Introduction Coronavirus disease 2019 (COVID-19) is the greatest worldwide pandemic of the 21st century caused by the highly infectious SARS-CoV-2. According to the World Health Organization (WHO), SARS-CoV-2 has caused more than 768.2 million cases worldwide to date (WHO, 2022b). Although COVID-19 vaccines are being developed rapidly, compared to traditional…

Coronavirus disease (COVID-19) – World Health Organization. www.who.int/emergencies/diseases/novel-coronavirus-2019. Harvey, W. T. et al. SARS-CoV-2 variants, spike mutations and immune escape. Nat. Rev. Microbiol. 19, 409–424 (2021). Article  CAS  PubMed  PubMed Central  Google Scholar  Kang, L. et al. A selective sweep in the Spike gene has driven SARS-CoV-2 human adaptation. Cell…

For what would have been Henrietta Lacks’ 103rd birthday last month, her family settled a lawsuit with Thermo Fisher –  a multinational biotech company that had used her cells without seeking consent for several years – who regarded the win as a “fitting day” for justice. While the settlement took…

Abstract Protein-protein interactions are central, highly flexible components of regulatory mechanisms in all living cells. Over the years, diverse methods have been developed to map protein-protein interactions. These methods have revealed the organization of protein complexes and networks in numerous cells and conditions. However, these methods are also time consuming,…

In the present study, we selected AXL, NRP1, and ACE2 receptors to predict the potential susceptibility of SARS-CoV-2 VOCs to chickens. Based on the analysis using protein sequences and models, we predicted that AXL and ACE2 receptors in chickens could be used as binding sites for emerging SARS-CoV-2 variants in…

Hooper, N. M., Lambert, D. W. & Turner, A. J. Discovery and characterization of ACE2—A 20-year journey of surprises from vasopeptidase to COVID-19. Clin. Sci. 134, 2489–2501 (2020). Article  CAS  Google Scholar  Hamming, I. et al. The emerging role of ACE2 in physiology and disease. J. Pathol. 212, 1–11 (2007)….

Sample collection Previously, we collected nasal samples from WTD in northeastern Ohio focusing on metropolitan area parks11. In the present study, we expanded geographical study area by approximately 1000-fold to target the entire state of Ohio. We collected 1522 nasal swabs from WTD across 83 of Ohio’s 88 counties from…

Protein expression hACE2-hIgG1 was constructed by fusing the extracellular domain of human ACE2 (amino acid 19–615) (UniProt Q9BYF1) to the human IgG1 hinge and Fc (UniProt P01857). H374N and H378N mutations were introduced to inactivate the Zinc metallo-enzyme activity17. HH-120 was constructed by fusing hACE2-hIgG1 with an IgM tailpiece (PTLYNVSLVMSDTAGTCY)33….

Since the early months of the COVID-19 pandemic, scientists have investigated whether ABO blood group is related to the risk of SARS-CoV-2 infection and illness. iStock.com/vladm After all, nearly 20 years ago, researchers reported that type O blood was associated with a lower risk of the original SARS. In addition,…

The COVID-19 pandemic caused by SARS-CoV-2 has resulted in millions of confirmed cases and deaths worldwide. Understanding the biological mechanisms of SARS-CoV-2 infection is crucial for the development of effective therapies. This study conducts differential expression (DE) analysis, pathway analysis, and differential network (DN) analysis on RNA-seq data of four…

1. Introduction Tropospheric ozone (O3) is a ubiquitous, highly reactive oxidant pollutant produced by photochemical reactions between nitrogen oxides and volatile organic compounds in the presence of ultraviolet light [1]. Ambient O3 levels are regulated by the U.S. Environmental Protection Agency under the Clean Air Act, with the current National…

Introduction SARS-CoV-2 is the most lethal virus among all human coronavirus (HCoV) and is responsible for causing severe respiratory tract infections.1,2 It was first reported in Wuhan, China, at the end of December 200193–5 and found to be highly transmissible (Basic reproduction number ranging from 0.9 to 6.53).4,6 The genome…

Wang D, Hu B, Hu C, Zhu F, Liu X, Zhang J, et al. Clinical characteristics of 138 hospitalized patients with 2019 novel coronavirus–infected pneumonia in Wuhan. China JAMA. 2020;323(11):1061–9. Article  CAS  PubMed  Google Scholar  Zhao Y, Zhao Z, Wang Y, Zhou Y, Ma Y, Zuo W. Single-cell RNA expression…

Ethics All animal experiments were conducted in accordance with the University of Tokyo’s Regulations for Animal Care and Use, which were approved by the Animal Experiment Committee of the Institute of Medical Science, the University of Tokyo. The committee acknowledged and accepted both the legal and ethical responsibility for the…

In a recent study published in Nature, researchers identified the anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) function of phospholipid scramblase 1 (PLSCR1), a cell-autonomous restriction factor induced by interferon-gamma (IFNγ). Its identification will help enhance the understanding of protective immunity in the human IFN response. The study used genome-wide…

Hirotsu, Y. et al. SARS-CoV-2 Omicron sublineage BA.2 replaces BA.1.1: genomic surveillance in Japan from September 2021 to March 2022. J. Infect. 85, 174–211 (2022). Article  CAS  PubMed  PubMed Central  Google Scholar  Lyngse, F. P. et al. Household transmission of SARS-CoV-2 Omicron variant of concern subvariants BA.1 and BA.2 in…

In a brief report recently published in Blood, researchers evaluated the association between blood group A and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections. Study: Blood Group A Enhances SARS-CoV-2 Infection. Image Credit: Phonlamai Photo/Shutterstock.com Background SARS-CoV-2 infects millions worldwide, with ABO blood group antigens being the foremost polymorphism…

Sample collection Sampling kits were sent out to various bat rehabilitators in the UK, as described previously56, for the collection of faeces from bats. These faecal samples (0.02–1 g) were immediately stored in 5 ml of RNAlater solution to prevent degradation of RNA. The geographical locations and collection dates for all samples…

Sequencing-grade modified trypsin (Cat No. V5111) and chymotrypsin (Cat No. V1061) were purchased from Promega (Madison, WI). The SARS-CoV-2 spike proteins were purchased from R&D systems (St. Paul, MN) (Cat. No’s 10561-CV-100, 10796-CV-100, 10786-CV-100, 10795-CV-100, 10878-CV-100, and 11060-CV-100). Dithiothreitol (DTT), iodoacetamide (IAA) and α-lytic protease (Cat No. A6362) were purchased…

As a scientist, you have better things to do than waste your time searching for the products you need. Let us help you. As a distributor of life science solutions with more than 20 years of experience, we can help you find what you need. Having high quality products allows…

Kousathanas, A. et al. Whole genome sequencing reveals host factors underlying critical Covid-19. Nature. doi.org/10.1038/s41586-022-04576-6 (2022). Article  PubMed  PubMed Central  Google Scholar  COVID-19 HGI. & Ganna, A. Mapping the human genetic architecture of COVID-19: an update. medRxiv. doi.org/10.1101/2021.11.08.21265944 (2022). Horowitz, J. E. et al. Genome-wide analysis provides genetic evidence that…

Entry of the SARS-CoV-2 virus into human tissues depends on the activity of a host gene that regulates production of a key viral receptor, according to a study published in the open access journal PLOS Biology by Madison Strine and Craig Wilen of Yale University, U.S., and colleagues.  The finding…

Cell lines and culture A549-hACE2 [a gift from Dr. Ben tenOever3], 293 T, HEK293, A549, and 3T3-L1 pre-adipocyte cell lines (refer to Supplementary Table 1) were cultured in DMEM and 10% FBS. U937, Calu-3, and HCT116 cells were cultured in RPMI, EMEM, and McCoy’s 5 A media, respectively, with 10% FBS. Caco-2 cells…

Hunter, D. J. & Bierma-Zeinstra, S. Osteoarthritis. Lancet 393, 1745–1759 (2019). Article  CAS  PubMed  Google Scholar  Puig-Junoy, J. & Ruiz Zamora, A. Socio-economic costs of osteoarthritis: A systematic review of cost-of-illness studies. Semin. Arthritis Rheum. 44, 531–541 (2015). Article  PubMed  Google Scholar  Hunter, D. J., March, L. & Chew, M….

Assembly of T4 artificial viral vectors T4-AVVs were assembled by sequential incorporation of purified biomaterials to generate a virus structural mimic (Fig. 2a and Supplementary Movie 1). Starting with an empty capsid shell purified from E. coli infected by the neck-minus and tail-minus T4 phage mutant (10-amber.13-amber.HocΔ.SocΔ T4) (Supplementary Fig. 1a), a pentameric…

Study cohorts This study was conducted in compliance with the Declaration of Helsinki and was approved by the Maoming People’s Hospital (Approval No. 2021-hs-43) and Dongguan Ninth People’s Hospital (Approval No. 2022-8). COVID-19 was confirmed based on positive results of reverse transcription-polymerase chain reaction (RT-PCR) of nasopharyngeal samples. Whole blood…

Over 6.93 million deaths have occurred due to critical illness following infection with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the agent responsible for the coronavirus disease 2019 (COVID-19). The lack of reliable predictive markers has made it difficult to triage cases requiring more intensive care before they become…

Ethics statement All experiments with hamsters were performed in accordance with the Science Council of Japan’s Guidelines for the Proper Conduct of Animal Experiments. The protocols were approved by the Institutional Animal Care and Use Committee of National University Corporation Hokkaido University (approval ID: 20-0123 and 20-0060). All protocols involving…

Abdel-Basset M, Hawash H, Elhoseny M, Chakrabortty RK, Ryan M (2020) DeepH-DTA: deep learning for predicting drug-target interactions: a case study of COVID-19 drug repurposing. IEEE Access 8:170433–170451 Article  Google Scholar  Abdulrahman SA, Salem ABM (2020) A efficient deep belief network for Detection of Corona Virus Disease COVID-19. Fusion Pract,…

1. Introduction The World Health Organization (WHO), on 11 March 2020, declared the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) as a pandemic [1,2]. The causative virus is a newly discovered pathogenic member of the beta coronavirus genus, which causes the coronavirus disease 2019 (COVID-19). The pathogen has primarily been…

SARS-CoV-2 infection induces mtDNA release and recognition by cGAS Recent studies revealed that SARS-CoV-2 stimulation could cause the accumulation of intracellular and intercellular DNA due to cell fusion, cell death and even tissue damage.17,19,27 To detect SARS-CoV-2-triggered release of DNA during infection, we first detected the cytosolic dsDNA pattern by…

Nearly 90% of the disease risk-associated variants identified by genome-wide association studies are in non-coding regions of the genome. The annotations obtained by analyzing functional genomics assays can provide additional information to pinpoint causal variants, which are often not the lead variants identified from association studies. However, the lack of…

Acquiring amino acid substitutions via language model consensus We select amino acid substitutions recommended by a consensus of language models. We take as input a single wild-type sequence x = (x1,…,xN)∈ \(\mathcal{X}\)N, where \(\mathcal{X}\) is the set of amino acids, and N is the sequence length. We also require a set of…

April 19, 2023 | Judicial Watch (Washington, DC) – Judicial Watch announced today it received 552 pages of records from the U.S. Department of Health and Human Services (HHS) which include the initial grant application and annual reports to the National Institutes of Health (NIH) from EcoHealth Alliance, describing the…

IgG plasmids Antibody sequences and Fc-tagged ACE2 were cloned into the CMV/R plasmid backbone for expression under a CMV promoter. The antibodies with variable HC/LC were cloned between the CMV promoter and the bGH poly(A) signal sequence of the CMV/R plasmid to facilitate improved protein expression. The variable region was…

To KK-W, Sridhar S, Chiu KH-Y, Hung DL-L, Li X, Hung IF-N, Tam AR, Chung TW-H, Chan JF-W, Zhang AJ-X, et al. Lessons learned 1 year after SARS-CoV-2 emergence leading to COVID-19 pandemic. Emerg Microbes Infect. 2021;10(1):507–35. Article  CAS  PubMed  PubMed Central  Google Scholar  Gao Q, Bao L, Mao H,…

*Important notice: bioRxiv publishes preliminary scientific reports that are not peer-reviewed and, therefore, should not be regarded as conclusive, guide clinical practice/health-related behavior, or treated as established information. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, which is otherwise known as the coronavirus disease 2019 (COVID-19), continues to pose significant…

Lentiviral system approach and antiviral activities of the test drugs against SARS-CoV-2 and its variants delta and omicron in vitro. (a) HEK293 cells were transfected with a plasmid encoding a lentiviral backbone (genome) expressing a marker protein, a plasmid expressing spike proteins of SARS-CoV-2 and its variants delta and omicron,…

Research reported in this study complies with all relevant ethical regulations, and our animal study protocol has been approved by the Animal Experimental Board of Finland (license number ESAVI/28687/2020. Phage panning Sherpabody phage display library (size ~1011 cfu) was obtained from Next Biomed Therapies Oy. To develop sherpabodies specific for SARS-CoV-2…

Cell culture Human lung adenocarcinoma cells A549 expressing human ACE2 (A549-ACE2, NR-53821) were acquired from BEI Resources. They were maintained in DMEM/F-12 (1:1, Corning) medium supplemented with 10% FBS (GeneDepot) and blasticidin (100 μM). Normal A549 cells were purchased from ATCC (CCL-185) and cultured in DMEM/F-12 (1:1, Corning) supplemented with 10%…

Reagents, consumables, and kits The oligonucleotides used as primers in the asymmetric polymerase chain reaction (aPCR) and as staple strands for the folding of PB NPs, along with the CpG ODN 1018 adjuvant were purchased from Integrated DNA Technologies (IDT). Fluorescently labelled oligonucleotides used in FRET assay, FAM (donor) and…

In a recent article published in Nature Genetics, researchers showed that mammalian SWItch/Sucrose Non-Fermentable (mSWI/SNF) chromatin remodeling complexes represent a potential class of host-directed broad-acting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) therapeutic target. Study: Pharmacological disruption of mSWI/SNF complex activity restricts SARS-CoV-2 infection. Image Credit: Kateryna Kon/Shutterstock Background Chromatin…

Figure 8 shows the ACE2 protein downloaded from AlphaFold  with the UniProt ID of A0A7I2V4H0. ACE2 protein is being visualized in the Chimera application with regions in red showing the residues believed to be involved in the interations with SARS-CoV2 virus as per the paper by Lan et al. (2020)….

Bergwerk, M. et al. Covid-19 breakthrough infections in vaccinated health care workers. N. Engl. J. Med. 385, 1474–1484 (2021). Article  CAS  PubMed  Google Scholar  V’Kovski, P., Kratzel, A., Steiner, S., Stalder, H. & Thiel, V. Coronavirus biology and replication: implications for SARS-CoV-2. Nat. Rev. Microbiol. 19, 155–170 (2021). Article  PubMed …

What gets hyped, and what remains neglected, often depends on good storytelling. When the Human Genome Project began, in 1990, it had a simple story, well told. From a standing start, American taxpayers would pay for an exhaustive map of the DNA that makes up the 24 sorts of chromosomes…

Keywords SARS-CoV-2, COVID-19, cyclic guanosine monophosphate-adenosine monophosphate synthase-stimulator of interferon genes pathway, lung damage, interferon, cytokines Introduction Severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) caused the coronavirus disease 2019 (COVID-19) pandemic with origin in Wuhan, China [1]. With confirmed 522 million infections and death of 6.27 million until May…

Entry K24152                      KO                                      Symbol S Name SARS coronavirus spike glycoprotein Pathway map05171   Coronavirus disease – COVID-19 Network nt06171  Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)nt06513  Complement cascade   Element N01307   SARS-CoV-2 S to AngII-AT1R-NOX2 signaling pathway N01312   SARS-CoV-2 S to lectin pathway of complement cascade N01314   SARS-CoV-2 S to classical pathway of complement cascade…

Data reporting No statistical methods were used to predetermine sample size. The experiments were not randomized. The investigators were not blinded to allocation during experiments and outcome assessment. Study approval This study and all the relevant experiments were approved by Guangzhou Eighth People’s Hospital Ethics Committee (No. 202001134 and 202115202)….

Summary of a Single-cell Guided Pipeline to Aid Repurposing of Drugs Using scRNA-seq data, ASGARD repurposes drugs for disease by fully accounting for the cellular heterogeneity of patients (Fig. 1, Formula 1 in “Methods” section). In ASGARD, every cell cluster in the diseased sample is paired to that in the normal…

In a recent study posted to the bioRxiv* preprint server, researchers cloned and functionally characterized the virus ribonucleic acid (RNA) sensor, retinoic acid-inducible gene-I (RIG-I), from the microbat Myotis daubentonii (Yangochiroptera suborder) and the megabat Rousettus aegyptiacus (Yinpterochiroptera suborder), and compared them to the human ortholog. Study: Functional comparisons of…

Infectious diseases, believed to be a thing of the past only a few decades ago owing to the discovery of antibiotics, have resurfaced as an area of active research with the rampant spread of antimicrobial resistance and evolving viruses. Caused by pathogens ranging in size from viruses to worms that…

In a recent study published in the PLOS Biology, researchers found that leucine-rich repeat-containing protein 15 (LRRC15), a toll-like receptor (TLR)-related cell surface receptor, blocked severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) binding. Study: Fibroblast-expressed LRRC15 is a receptor for SARS-CoV-2 spike and controls antiviral and antifibrotic transcriptional…

Credit: CROCOTHERY/Getty Images Researchers at the University of Sydney, Australia, have uncovered a lung protein that blocks SARS-CoV-2 infection and could be a new pathway for the development of drugs to treat COVID-19. This protein, called the leucine-rich repeat-containing protein 15 (LRRC15), is an inbuilt receptor that binds SARS-CoV-2 without…

(A) Schematic of CRISPRa screen used to identify regulators of SARS-CoV-2 spike binding. (B) Ranking of all genes in screen 1 by log2 fold change (LFC) calculated using MAGeCK and plotted using MAGeCKFlute. See also S1 Table. (C) Gene enrichment analysis of screen 1 performed using MAGeCK. Horizontal dotted line…

Ou A, Yung WKA, Majd N. Molecular mechanisms of treatment resistance in glioblastoma. Int J Mol Sci. 2021;22(1):351. CAS  Google Scholar  Wang G-M, Cioffi G, Patil N, Waite KA, Lanese R, Ostrom QT, et al. Importance of the intersection of age and sex to understand variation in incidence and survival…

doi: 10.1002/mco2.208. eCollection 2023 Feb. Zongzhi Liu  1   2   3 , Xiang Gao  4 , Chuanwen Kan  2   5 , Lingyu Li  1   2   3 , Yuan Zhang  6 , Yibo Gao  7 , Shengyuan Zhang  4 , Liangji Zhou  6 , Hui Zhao  6   8   9 , Mingkun Li  2  …

In a recent study posted to the bioRxiv* preprint server, researchers evaluated the impact of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) on the peripheral nervous system (PNS). Study: Human iPS cell-derived sensory neurons can be infected by SARS-CoV-2 strain WA1/2020 as well as variants delta and omicron. Image Credit:…

Cells and viruses hACE2 expressing BHK-21 (BHK-21-hACE2) and Vero-E6 cells were provided by State Key Laboratory of Virology, Wuhan University. Vero-E6 cells were cultured in DMEM (Gibco, Cat.11995–040) supplemented with 10% fetal bovine serum (Gibco, Cat.10091–148) and 1% penicillin and streptomycin solution (BasalMedia, Cat.S110JV) at 37°C with 5% CO2. BHK-21-hACE2…

Animals and immunizations The study protocol and all veterinarian procedures were approved by the Bioqual IACUC per a memorandum of understanding with the Duke IACUC, and were performed based on standard operating procedures. Macaques studied were housed and maintained in an Association for Assessment and Accreditation of Laboratory Animal Care-accredited…

In a recent study posted to the bioRxiv* preprint server, researchers developed a three-dimensional (3D) in vitro tissue model to assess the effects of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) on the vascular endothelial cells. Study: SARS-CoV-2 spike protein induces endothelial dysfunction in 3D engineered vascular networks. Image Credit:…

Ill African American girl blowing a nose at home while her mother is consoling her. getty This story on CRISPR is part of an extended series on Regenerative Medicine. For other stories on this topic see williamhaseltine.com and search for Regenerative Medicine. My definition of Regenerative Medicine is any medical…

In a recent study posted to the bioRxiv* preprint server, researchers assessed the impact of pre-existing immunity against severe acute respiratory coronavirus 2 (SARS-CoV-2) acquired by previous infections (PI) or coronavirus disease 2019 (COVID-19) vaccination on the transmission of SARS-CoV-2 variants of concern (VOCs) such as Delta and Omicron. Study:…

A visualization of the immune engineering process, from structure selection to authentic virus neutralization. Upper left: Analysis of interaction between SARS-CoV-2 and the ACE2 receptor revealed numerous contact residues along the receptor binding motif (RBM) ridgeline. Upper right: Focus was placed on residues comprising the FNCY patch, a conserved site…

A new set of strategies is being investigated to combat coronavirus disease 2019 (COVID-19), as well as other viral pandemics in the future. This includes the use of new antigens, adjuvants, and delivery systems. A new one MedComm The journal study describes the potential use of circular ribonucleic acid (circRNA)…

CHICAGO – A first-of-its-kind atlas of “ramp sequences” near the 5′ end of highly expressed genes promises to help researchers better understand gene expression and disease development. Bioinformaticians and computational biologists at the University of Kentucky and Brigham Young University recently released the Ramp Atlas, a compendium of 18,388 tissue-…

Cracking the Code for SARS-Cov2 and the Discovery of CRISPR-Cas Gene Editing Mechanism SARS-Cov2 1.) Describe the morphological structure of SARS-Cov2 virus. – According to studies through the use of Electron microscopy, the structure of SARS-CoV-2 is speculated to be similar to that of SARS-CoV with a virion size ranging…

The mass vaccination programs against coronavirus disease 2019 (COVID-19) received massive media attention and were widely credited for reducing the threat of the disease. However, many countries struggle to obtain enough doses to immunize their population, and some immunocompromised individuals are less protected by the vaccines. There is still a…

Researchers have confirmed that it’s not the SARS-CoV-2 virus itself damaging the body’s cells, it’s the spike protein. And it does this by binding ACE2, which can lead to mitochondrial damage. STORY AT-A-GLANCE Doctors for COVID Ethics have been warning about the potential for gene-based COVID-19 “vaccines” to cause blood…

ACE2 Protein, Human, Recombinant (hFc Tag), Biotinylated: Product Information Purity > 95 % as determined by SDS-PAGE. Activity Testing in progress Protein Construction A DNA sequence encoding the extracellular domain (Met 1-Ser 740) of human ACE2 precursor (NP_068576.1) was expressed with the fused Fc region of human IgG1…

In a recent study posted to the bioRxiv* preprint server, researchers assessed the impact of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variant on infected human cells and organs. Study: SARS-CoV-2 Omicron BA.1 variant infection of human colon epithelial cells. Image Credit: PHOTOCREO Michal Bednarek/Shutterstock Various studies have…

Single guide ribonucleic acid (sgRNA) molecules are produced by discontinuous transcription, in which viral RNA-dependant RNA polymerase pauses early negative-sense RNA synthesis and then jumps to the other end of the genome. The specifics of this process are still not fully understood. Since sgRNAs can play an important role in…

The coronavirus disease 2019 (COVID-19) pandemic caused by the severe acute respiratory syndrome-associated coronavirus 2 (SARS-CoV-2) and past SARS-CoV outbreaks were a result of zoonotic spillover of sarbecoviruses from animals to humans. Although most of the animal sarbecoviruses do not infect eukaryotic cells, clade 4 sarbecoviruses recently found in China…

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