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Tag: APC
cfDNA Extraction Sensitivity Controls – cfDNA Extraction Sensitivity Controls
Does your cfDNA extraction process affect the Limit of Detection (LOD) of a particular gene target? Our new products, cfDNA Extraction Sensitivity Panels and Extraction Low Positive Controls, can help your research and development for liquid biopsy assays. NEW PRODUCTS: cfDNA EXTRACTION SENSITIVITY PANELS & CONTROLS The Extraction Sensitivity Controls…
Apoptosis-resistant megakaryocytes produce large and hyperreactive platelets in response to radiation injury | Military Medical Research
Animals C57BL/6-Tg (Pf4-icre) Q3Rsko/J (Pf4-Cre) mice were purchased from the Jackson Laboratory (Bar Harbor, ME, USA). B6;129-Tmem173tm1(flox)Smoc (Stingfl) mice were purchased from Shanghai Model Organisms Center, Inc. (Shanghai, China). C57BL/6J-Ifnar1em1(flox)Cya (Ifnar1fl) mice were purchased from Cyagen Biosciences (Guangzhou, China). Stingcko and Ifnar1cko mice were generated by crossing Pf4-Cre mice with…
Preclinical assessment of an anti-HTLV-1 heterologous DNA/MVA vaccine protocol expressing a multiepitope HBZ protein | Virology Journal
Design of the recombinant virus MVA-HBZ The multiepitope protein design was based on HBZ sequence deposited in the GenBank database [17, 23, 24], and in silico analyses performed using the epitope prediction tools: NetMHCpan 4.0 (Threshold: weak binders—2.0; strong binders—0.5), NetCTL 1.2 (Threshold of 0.75) and IEDB—MHC-I Binding Predictions. Epitopes…
Potent latency reversal by Tat RNA-containing nanoparticle enables multi-omic analysis of the HIV-1 reservoir
Participants and blood collection A total of n = 23 HIV-1 seropositive individuals on stably suppressive ART were included in this study (Supplementary Table 1). Participants were recruited at Ghent University Hospital. 2/23 individuals are female, 21/23 are male; the limited representation of female individuals in our study is a direct reflection of…
What happens when cGPDH is treated with siRNA?
What is the time point for cell harvest post siRNA treatment via lipofectamine mediated transfection in adipocytes?4 answersThe time point for cell harvest post siRNA treatment via lipofectamine mediated transfection in adipocytes varies depending on the study. In one study by Kilroy et al., cells were harvested 48 hours post-transfection….
Whole-genome sequencing reveals the molecular implications of the stepwise progression of lung adenocarcinoma
Whole-genome sequencing (WGS) analysis of early and advanced adenocarcinomas Whole-genome short read and long read sequencing datasets of 76 lung cancer specimens were analyzed. The datasets included newly generated data for 48 early small-sized lung adenocarcinoma cases (collectively called “Early-Ad” hereafter). These cases included 26 AIS (9 and 17 cases…
Inhibition of O-GlcNAc transferase activates type I interferon-dependent antitumor immunity by bridging cGAS-STING pathway
Abstract The O-GlcNAc transferase (OGT) is an essential enzyme that mediates protein O-GlcNAcylation, a unique form of posttranslational modification of many nuclear and cytosolic proteins. Recent studies observed increased OGT and O-GlcNAcylation levels in a broad range of human cancer tissues compared to adjacent normal tissues, indicating a universal effect…
Genomic Predictors Associated With Exceptional Response to Systemic Therapy in Advanced Pancreatic Cancer
ABSTRACT Introduction: Exceptional response to therapy is rare in patients with advanced pancreatic cancer. This study explored potential genomic differences between typical and exceptional responses that could confer more favorable biology. Methods: We included exceptional responders and controls with advanced pancreatic cancer from Cleveland Clinic from April 2013 to August…
Special Issue : Extracellular Vesicles and ncRNA
Dear Colleagues, Over the past two decades, the biological science arena has witnessed the emergence and rapid advancement of two distinct research domains: noncoding RNA (ncRNA) and extracellular vesicles. Presently, the significance of ncRNA in the biology of virtually all living organisms is beyond dispute, and our comprehension of its…
Racial, ethnic disparities in mortality risk shown among younger patients with DLBCL
December 11, 2023 3 min read Add topic to email alerts Receive an email when new articles are posted on Please provide your email address to receive an email when new articles are posted on . ” data-action=subscribe> Subscribe We were unable to process your request. Please try again later….
Speaker Abbas distributes palliatives to 26,619 people in Zaria
A total of 26,619 people are to benefit from Phase 2 of palliatives distribution kicked off by the Speaker of the House of Representatives, Rt. Hon. Abbas Tajudeen, in the Zaria Federal Constituency of Kaduna State over the weekend. This brings the total number of beneficiaries for the Speaker’s palliatives…
Criteria for better assessment of rare genetic variants that can lead to hereditary colorectal cancer
APC gene, APC protein, and criteria boundaries and genotype-phenotype correlations. Representation of the APC gene (bottom) and its main protein product (middle) on the reference sequence NM_000038.6 (non-coding exon 1 not shown). The figure shows on the top the boundaries for the application of PVS1, BS3 and BP1 and genotype-phenotype…
APC Anti-EpCAM antibody [EPR20532-225] (ab237398)
Rabbit Recombinant Monoclonal EPCAM antibody – conjugated to APC. Validated in Flow Cyt and tested in Human samples. Alternative names= EGP, ESA, KSA, gp4, AUA1, EGP2, Ly74, M1S2, M4S1, MK 1, 17 1A, CD326, CO17A, DIAR5, EGP 2, EGP40, EPCAM, KS1/4, M1S 1, MIC18, TACD1, TROP1, 323/A3, EGP314, Ep CAM,…
Mitophagy in human health, ageing and disease
Palikaras, K., Lionaki, E. & Tavernarakis, N. Mechanisms of mitophagy in cellular homeostasis, physiology and pathology. Nat. Cell Biol. 20, 1013–1022 (2018). Article CAS PubMed Google Scholar Palikaras, K., Lionaki, E. & Tavernarakis, N. Coordination of mitophagy and mitochondrial biogenesis during ageing in C. elegans. Nature 521, 525–528 (2015). Article …
Exploring the promising potential of induced pluripotent stem cells in cancer research and therapy | Molecular Cancer
Rowe RG, Daley GQ. Induced pluripotent stem cells in disease modelling and drug discovery. Nat Rev Genet. 2019;20:377–88. Article CAS PubMed PubMed Central Google Scholar Li L, Papadopoulos V. Advances in stem cell research for the treatment of primary hypogonadism. Nat Rev Urol. 2021;18:487–507. Article CAS PubMed Google Scholar Lawrence…
Biophysical properties of NaV1.5 channels from atrial-like and ventricular-like cardiomyocytes derived from human induced pluripotent stem cells
hiPSC cultures and cardiomyocyte differentiation The hiPSC lines CBRCULi001-A54 and CBRCULi008-A55 were generated from a 44-year-old male and 75-year-old female control lymphoblastoids, respectively, and they were reprogramed at the LOEX core facility (Quebec City, QC, Canada). All the work with hiPSCs were approved by CIUSSS de la Capitale-Nationale ethics committee (Project…
Immune-privileged tissues formed from immunologically cloaked mouse embryonic stem cells survive long term in allogeneic hosts
Mice C57BL/6N (strain 005304), C3H/HeJ (strain 000659), FVB/NJ (strain 001800), BALB/cJ (strain 000651) and NSG mice (stock 005557) were purchased from the Jackson Laboratory. CD-1 (stock 022) mice were purchased from Charles River. Mice (6–20-week-old) of each strain/background were used for teratoma assays. Mice were housed in a pathogen-free facility…
Chromatin priming elements direct tissue-specific gene activity before hematopoietic specification
Introduction The development of multicellular organisms requires the activation of different gene batteries which specify the identity of each individual cell type. Such shifts in cellular identity are driven by shifts in the gene regulatory network (GRN) consisting of transcription factors (TFs) binding to the enhancers and promoters of their…
31L-APC-100ul | Interleukin 2 Receptor, alpha (IL-2Ra, IL-2R alpha)
Cat# I7663-31L-APC-100ul Size : 100ul Brand : US Biological Host mouse Conjugate APC Isotype IgG1 Clone Number 10B1132 (IL-A111) Grade Affinity Purified Applications IHC IP Shipping Temp Blue Ice Storage Temp 4°C Do Not Freeze Applications: Suitable for use in Immunohistochemistry, Immunoprecipitation and Functional Assays. Other applications not tested. Recommended…
Megakaryocytes possess a STING pathway that is transferred to platelets to potentiate activation
Introduction Host defence against infection relies on two important systems: the immune and the coagulation systems. Platelets are anucleate cells produced by megakaryocytes and are responsible for blood clotting and haemostasis (Lefrancais et al, 2017). They are also very abundant and play unexpected roles in immune responses (Maouia et al,…
Elevated stress response marks deeply quiescent reserve cells of gastric chief cells
Generation of inducible H2b-GFP knock-in mice Generation of inducible H2b-GFP knock-in mice was performed by using CRISPR/Cas943. In brief, a mixture containing a sgRosa26-1 crRNA43 (8.7 ng/μl, Fasmac, Japan), a tracrRNA (14.3 ng/μl, Fasmac, Japan), a single strand oligo donor nucleotide (ssODN) composed of 5′ arm, adenovirus splicing acceptor, SV40 pA, TRE3G…
APC anti-human CD326 EpCAM Antibody anti-CD326
Description CD326 is also known as EpCAM, tumor associated calcium signal transducer 1, epithelial cell surface antigen, epithelial glycoprotein 2, EGP2, adenocarcinoma associated antigen, and TROP1. CD326 is a type I transmembrane protein containing six disulfide bridges and one THYRO domain. This cell surface glycosylated 40 kD protein is highly…
AB-F1181UC-25 | FITC Anti-Mouse CD326 Antibody[G8.8] Clinisciences
Product Name Sizes Price FITC Anti-Mouse CD326/EpCAM Antibody[G8.8] 50Tests, 100Tests, 100Tests×2 Check More PE Anti-Mouse CD326/EpCAM Antibody[G8.8] 50Tests, 100Tests, 100Tests×2 Check More APC Anti-Mouse CD326/EpCAM Antibody[G8.8] 50Tests, 100Tests, 100Tests×2 Check More PE/Cyanine7 Anti-Mouse CD326/EpCAM Antibody[G8.8] 50Tests, 100Tests, 100Tests×2 Check More Elab Fluor® 488 Anti-Mouse CD326/EpCAM Antibody[G8.8] 50Tests, 100Tests, 100Tests×2 Check More PE Anti-Mouse CD326/EpCAM Antibody[G8.8] 25μg, 100μg Check More APC Anti-Mouse…
Treatment of the Monocyte-Derived Dendritic Cells (mo-DCs) with Sialidase
Collect approximately 10 times 10 to the sixth human monocyte derived dendritic cells differentiated from the monocytes. From the 10 wells of the 24 well plate having 1.3 times 10 to the sixth cells per well, transfer them to a new sterile 15 milliliter conical tube. After centrifuging the cells…
Author Spotlight: Dendritic Cells Maturation Using Sialidases-Based Enzymatic Treatment of the Cell Surface
The scope of the glycoimmunology group research is studying the role of glycan immunology in human health and disease. This research aims to disentangle biological mechanisms modulated by glycosylations, and to discover novel glycan-based therapies for cancer and congenital disorders of glycosylation. Our focus has been on sialylated glycans that…
Primate-specific ZNF808 is essential for pancreatic development in humans
Subjects The study was conducted in accordance with the Declaration of Helsinki and all subjects or their parents/guardian gave informed written consent for genetic testing. DNA testing and storage in the Beta Cell Research Bank was approved by the Wales Research Ethics Committee 5 Bangor (REC 17/WA/0327, IRAS project ID…
Single-cell CRISPR screens in vivo map T cell fate regulomes in cancer
Mice The research conducted in this study complied with all of the relevant ethical regulations. The animal protocols were approved by and performed in accordance with the Institutional Animal Care and Use Committee of St. Jude Children’s Research Hospital. C57BL/6, OT-I50, pmel51 and Rosa26-Cas9 knock-in52 mice were purchased from The…
Rapid purification and characterization of circulating small extracellular vesicles on a label-free lab-on-a-chip
Device layout and graphical outline of experimental flow. Small extracellular vesicles (sEVs) were simultaneously extracted from human biofluids using the insulator-based dielectrophoretic (iDEP) device, wherein a trapping zone was created at the tip region of micropipettes by balancing the dielectrophoretic (DEP) force with two electrokinetic forces, including electroosmosis (EOF) and…
Biological and genetic characterization of a newly established human external auditory canal carcinoma cell line, SCEACono2
Ethic statement The Clinical Research Ethics Review Committee of Kyushu University Hospital approved the study (permit no. 29-43, 30-268, and 700-00). Written informed consent for the current research project was obtained before the tumor tissue, and a blood sample were harvested. This study was also conducted according to the principles…
An FcRn-targeted mucosal vaccine against SARS-CoV-2 infection and transmission
Mice and Golden Syrian hamsters All research experiments in this study comply with all relevant ethical regulations. The Institutional Animal Care and Use Committee approved the animal protocol at the University of Maryland (#R-APR-20-18 and #R-MAR-21-19). The animals were acclimatized at the animal facility for 4–6 days before initiating experiments….
Rapid and sensitive detection of Pseudomonas aeruginosa by isothermal amplification combined with Cas12a-mediated detection
P. aeruginosa is a common pathogenic bacterium, which seriously threaten human health12,13. In 2017, the World Health Organization (WHO) added P. aeruginosa to the short list of antibiotic-resistant pathogens, the so-called ESKAPE group of pathogens. Traditional pathogenic microbial detection methods require the isolation, culture, and biochemical identification of pathogenic bacteria,…
Advancements in Rift Valley fever vaccines: a historical overview and prospects for next generation candidates
Gerken, K. N. et al. Paving the way for human vaccination against Rift Valley fever virus: a systematic literature review of RVFV epidemiology from 1999 to 2021. PLoS Negl. Trop. Dis. 16, e0009852 (2022). Article CAS PubMed PubMed Central Google Scholar Pepin, M., Bouloy, M., Bird, B. H., Kemp, A….
The Atoh1-Cre Knock-In Allele Ectopically Labels a Subpopulation of Amacrine Cells and Bipolar Cells in Mouse Retina
Abstract The retina has diverse neuronal cell types derived from a common pool of retinal progenitors. Many molecular drivers, mostly transcription factors, have been identified to promote different cell fates. In Drosophila, atonal is required for specifying photoreceptors. In mice, there are two closely related atonal homologs, Atoh1 and Atoh7….
Quantification of rare somatic single nucleotide variants by droplet digital PCR using SuperSelective primers
Primary samples and nucleic acid extraction A cohort of 48 patients diagnosed with advanced adenoma (AAD), defined by size > 20 mm, or colorectal carcinoma (CRC) were collected between 2013 and 2016. The study was approved by the institutional ethics committee of Hospital General Universitario de Alicante (Ref. CEICPI2013/01), and written informed consent…
Does A315T mutation of TDP43 causes cytoplasmic aggregates in hek293 cells?
The A315T mutation of TDP43 does not cause cytoplasmic aggregates in HEK293 cells. The A315T mutation is one cause of familial amyotrophic lateral sclerosis (ALS) and is associated with cytoplasmic aggregates in neurons of ALS patients. However, the formation of cytoplasmic aggregates in HEK293 cells is not observed in the…
Diet-induced rewiring of the Wnt gene regulatory network connects aberrant splicing to fatty liver and liver cancer in DIAMOND mice
DIAMOND mice develop HCC in the context of obesity and insulin resistance To investigate in depth the genome-wide transcriptional and epigenetic changes that occur during development of MAFLD and obesity-related hepatocellular carcinoma (HCC), we performed RNA-sequencing (RNA-seq) and Chromatin ImmunoPrecipitation-sequencing (ChIP-seq) of liver tumours (HCC) and liver control tissue (FL)…
APC Anti-EpCAM antibody [VU-1D9] (ab239288)
Mouse Monoclonal EPCAM antibody – conjugated to APC. Validated in Flow Cyt and tested in Human samples. Alternative names= EGP, ESA, KSA, gp4, AUA1, EGP2, Ly74, M1S2, M4S1, MK 1, 17 1A, CD326, CO17A, DIAR5, EGP 2, EGP40, EPCAM, KS1/4, M1S 1, MIC18, TACD1, TROP1, 323/A3, EGP314, Ep CAM, Ep-CAM,…
TET2 modulates spatial relocalization of heterochromatin in aged hematopoietic stem and progenitor cells
Animal models Animal studies were approved by the Institutional Animal Care Use Committee of the Institute of Biosciences and Technology, Texas A&M University (AUP 2020-0195). Most mouse strains bear a C57BL/6 genetic background unless otherwise noted. All the animals were housed in a certified animal facility with a standard dark/light…
Bioconductor – Bioconductor 3.18 Released
Home Bioconductor 3.18 Released October 25, 2023 Bioconductors: We are pleased to announce Bioconductor 3.18, consisting of 2266 software packages, 429 experiment data packages, 920 annotation packages, 30 workflows and 4 books. There are 69 new software packages, 10 new data experiment packages, 8 new annotation packages, no new workflows,…
Comparison of two lab-scale protocols for enhanced mRNA-based CAR-T cell generation and functionality
Experimental design Primary T cell isolation, expansion, and cell culture Primary T cells were isolated from human peripheral blood mononuclear cells (PBMCs) of healthy donors collected by the Institute for Transfusion Medicine of the University Clinic of Leipzig, Germany, approved by the Ethics Committee of the Faculty of Medicine of…
Pathway-driven analysis of synthetic lethal interactions in cancer using perturbation screens
Introduction Cancer cells are characterized by unrestrained proliferation and dysregulated growth, which lead to the formation of malignant neoplasms (Hanahan & Weinberg, 2023). The development and progression of cancer have been linked to the dysregulation of multiple signaling pathways, including MAPK/ERK, Wnt/β-catenin, PI3K/AKT/mTOR, and NF-kB, which are among crucial pathways…
Nykode Therapeutics to Present at the mRNA Cancer Vaccines Summit 2023 in Boston
Nykode Therapeutics ASA Nykode’s APC targeted vaccine technology can be delivered as mRNA and generate strong T cell responses Nykode’s APC targeted vaccine technology consistently results in faster, broader and stronger immune response compared to non-targeted vaccines, whether delivered as DNA or mRNA OSLO, Norway, Oct. 09, 2023 (GLOBE NEWSWIRE)…
Immunosuppression causes dynamic changes in expression QTLs in psoriatic skin
Mapping eQTLs in patients with psoriasis We obtained longitudinal lesional and non-lesional skin biopsies from participants at baseline, during treatment, and at the time of psoriasis relapse after study medication withdrawal over a course of 22 months. We used genome-wide genotyping and RNA-seq to assay samples. After stringent quality control,…
Diagnosing and monitoring pancreatic cancer through cell-free DNA methylation: progress and prospects | Biomarker Research
The exploration of methylated biomarkers in plasma for pancreatic cancer is still in its early stages, and the number of studies conducted to date for this purpose is limited. Analysis of cfDNA methylation patterns in pancreatic cancer has been approached both at the whole genome level and by identifying and…
FDA Grants Marketing Authorization to First DNA Test to Assess Genetic Predisposition to Select Cancers
The FDA has granted de novo marketing authorization for the Invitae Common Hereditary Cancers Panel, an in vitro diagnostic test designed to detect genetic variants associated with an elevated risk of developing certain types of cancer.1 Additionally, the panel could also help identify potential cancer-associated variants in patients who have…
The potential use of bacteria and bacterial derivatives as drug delivery systems for viral infection | Virology Journal
Novel approaches to treating viral illnesses must be developed. Drug delivery methods based on nanomaterials are attractive options in the search for therapeutic advantages because of their distinctive properties. Researchers should also examine how the most prevalent viruses, such as the flu, HIV, SARS-CoV-2, monkeypox, and HPVs, are being treated…
PTEN-induced kinase 1 gene single-nucleotide variants as biomarkers in adjuvant chemotherapy for colorectal cancer: a retrospective study | BMC Gastroenterology
Tissue samples A total of 84 analytic samples from surgical or biopsy specimens were collected from 84 patients who underwent radical surgery for CRC at Saitama Medical University International Medical Center between January and December 2016. One case was excluded because the specimen was too small; therefore, we used a…
Innovative strategies of reprograming immune system cells
Introduction Cancer is well characterized by genomic instability resulting in structural alterations that build up with tumor progression.1,2 A subpopulation of undifferentiated cancer cells, known as cancer stem cells (CSCs) are found within the tumor bulk. These cells are responsible for cancer initiation, recurrence and show therapeutic resistance and these…
IJMS | Free Full-Text | CRISPR-Cas9 Direct Fusions for Improved Genome Editing via Enhanced Homologous Recombination
Over the past decade, CRISPR-Cas9 has found widespread application in loss-of-function mutations, but precise genetic engineering for gene correction or gene replacement therapies has lagged behind. In vivo correction using CRISPR-Cas9 to replace genetic mutations by HR is highly challenging, and very few studies have managed to achieve this [31,32]….
KCNQ potassium channels modulate Wnt activity in gastro-oesophageal adenocarcinomas
Introduction The KCNQ (potassium voltage-gated channel subfamily Q) family of ion channels encode potassium transporters (1). KCNQ proteins typically repolarise the plasma membrane of a cell after depolarisation by allowing the export of potassium ions, and are therefore involved in wide-ranging biological functions including cardiac action potentials (2), neural excitability…
CRISPR-Cas12a mediated combinatorial knock-out of multiple epigenetically silenced genes promotes pro-tumorigenic properties in colon cancer evolution
Abstract Genome-wide epigenetic alterations are important for the development of cancers. In any given cancer tissue, multiple important developmental regulators undergo epigenetic silencing. Similar epigenetic alterations are observed in normal physiological aging. The cumulative effects of multiple epigenetically silenced genes (ESGs) on tumor development remains an unaddressed aspect of cancer…
Genome-Wide Association Study of Alopecia Areata in Taiwan
Introduction Alopecia areata (AA) is one of Taiwan’s most common autoimmune hair diseases and incidence rate of AA is 0.22%.1–3 The main symptoms of AA are rapid, non-scarring hair loss that affects body hair, facial hair, eyelashes, and brows.1,2 In the United States, the prevalence of AA is estimated to…
A biobank of pediatric patient-derived-xenograft models in cancer precision medicine trial MAPPYACTS for relapsed and refractory tumors
Patient characteristics and PDX establishment Between February 2016 and July 2020, 787 pediatric, adolescent and young adult patients with recurrent or refractory malignancies were enrolled in the MAPPYACTS trial;2 756 (96%) patients and their parents consented to the optional ancillary study of preclinical model development (Fig. 1a). 744 patients had a…
Mismatch repair deficiency is not sufficient to elicit tumor immunogenicity
Mice All animal use was approved by the Department of Comparative Medicine at the Massachusetts Institute of Technology (MIT) and the Institutional Animal Care and Use Committee under protocol no. 0714-076-17. Mice were housed with a 12-h light/12-h dark cycle with temperatures in the range 20–22 °C and 30–70% humidity. KrasLSL-G12D…
Epitope editing enables targeted immunotherapy of acute myeloid leukaemia
Sleeping Beauty transgene overexpression Bidirectional Sleeping Beauty plasmids encoding the codon-optimized cDNA of the gene of interest (FLT3, KIT, CD123) or their mutated variants were cloned downstream of an EEF1A1 promoter. The same construct included a reporter cassette comprising a fluorescent protein (mCherry or mTagBFP2 for FLT3 and KIT, respectively)…
Mutational order and epistasis determine the consequences of FBXW7 mutations during colorectal cancer evolution
Abstract Somatic driver mutations, in genes such as FBXW7, have been discovered in phenotypically normal colonic tissue, however their role in cancer initiation remains elusive. Here, using patient-derived human colon organoids as models of early tumour evolution we investigate the consequences of FBXW7 mutations in normal and gene-edited organoids. We…
Inhibition of tumor intrinsic BANF1 activates antitumor immune responses via cGAS-STING and enhances the efficacy of PD-1 blockade
Introduction Immune checkpoint inhibitors (ICIs) have revolutionized the treatment pattern of various cancers, including melanoma and non-small cell lung cancer (NSCLC).1–3 However, there are still a considerable proportion (40%–60%) of patients who do not respond or do not achieve durable responses to these drugs.4 Thus, it is urgent clinical need…
Translation dysregulation in neurodegenerative diseases: a focus on ALS | Molecular Neurodegeneration
Tandan R, Bradley WG. Amyotrophic lateral sclerosis: part 1. Clinical features, pathology, and ethical issues in management. Annals of Neurology: Official Journal of the American Neurological Association and the Child Neurology Society. 1985;18:271–80. Article CAS Google Scholar Mehta P et al. Prevalence of amyotrophic lateral sclerosis (ALS), United States, 2016….
Clinical Study of the Role of Differential Blood Count as a Pretreatment Diagnosis for Patients With Diffuse Large B Cell Lymphoma (DLBCL) | Wahab
Clinical Study of the Role of Differential Blood Count as a Pretreatment Diagnosis for Patients With Diffuse Large B Cell Lymphoma (DLBCL) John Wahab, Limei Ai, Yu Shanshan, Marcilinus Zekrumah Abstract The study employed simple differential blood count assessing clinical prognosis index at diagnosis of DLBCL patients. Each patient’s prognostic…
Ulcerative Colitis Treatment by CLP loaded with NLRP3 siRNA
Introduction Since the 20th century, tThe incidence of ulcerative colitis (UC) has been increasing over time, negatively affecting peoples’ health.1,2 UC is a multifactorial induced inflammatory bowel disease (IBD) characterized by chronic recurrent and remitting mucosal inflammation.3,4 It originates in the rectum and then extends to the proximal segments of…
Transient naive reprogramming corrects hiPS cells functionally and epigenetically
Cell culture All cell lines used and derived by different approaches in this study are listed in Supplementary Table 1. Detailed information about the experimental design, materials and reagents is presented in the Reporting Summary. Primary human adult dermal fibroblasts (HDFa) from three different female donors were obtained from Gibco…
Platelet factors attenuate inflammation and rescue cognition in ageing
Animal models The C57BL/6 mouse line was used for all of the experiments (The Jackson Laboratory and National Institutes of Aging). Homozygous Pf4-KO mice were previously generated and characterized as described48. Pf4-KO mice were a gift from M. Anna Kowalska. Heterozygous mice were bred to generate Pf4-KO and WT littermate…
Characterizing the role of Phlda3 in the development of acute toxicity and malignant transformation of hematopoietic cells induced by total-body irradiation in mice
Mouse strains and procedures All animal procedures for this study were approved by the Institutional Animal Care and Use Committee (IACUC) at Duke University (protocol number A215-20-11 was approved on November 9, 2020). All methods were performed in accordance with the relevant guidelines and regulations. The study is reported in…
Dissecting human population variation in single-cell responses to SARS-CoV-2
Sample collection The individuals of self-reported African (AFB) and European (EUB) descent studied are part of the EVOIMMUNOPOP cohort18. In brief, 390 healthy male donors (188 AFB and 202 EUB) were recruited between 2012 and 2013 in Ghent (Belgium), thus, before the COVID-19 pandemic. Blood was obtained from the healthy…
Molecular reshaping of phage-displayed Interleukin-2 at beta chain receptor interface to obtain potent super-agonists with improved developability profiles
Soft-randomization of phage-displayed hIL-2 beta chain interface revealed hotspots modulating the interaction Soft-randomization was chosen for an initial mutational scanning at hIL-2/hIL-2R beta interface, focused on solvent-exposed (>20%) hIL-2 residues close to the beta chain (<5 Å). A library of 7 × 107 members was obtained by Kunkel mutagenesis15 with spiked mutagenic oligonucleotides…
Proteogenomic analysis reveals RNA as a source for tumor-agnostic neoantigen identification
Human study The study was approved by the institutional review boards (Ethics Commission of the Medical Faculty of Technical University Munich (protocol 193/17S) and Ethics Committee of the Medical Faculty of Heidelberg University (protocol S-206/2011)) and all patients provided written informed consent under these protocols. The study was conducted in…
Highlights from the 2023 Single-Cell and AI in Medicine Symposium
By Randall C. Willis with review and comments from Fabrice Chouraqui, Laurens Kruidenier, Chad Nusbaum, Diogo Camacho August 2, 2023 | “Technology and computation are revolutionizing the way we are creating and developing new drugs,” Cellarity CEO Fabrice Chouraqui announced to open the Second Single-Cell and AI in Medicine Symposium…
Aug. 1, 2023, San Diego Metro Magazine
Mid-Coast Corridor construction project named best in the U.S. By Kylie Capuano | sdnews.com The Mid-Coast Corridor construction project, responsible for extending the Blue Line Trolley to UTC and expanding transportation capacity in San Diego, was named the best construction project in the country for 2022. As a result, the…
explore breakthrough platforms for improved immunization
In a recent review published in Signal transduction and targeted therapy Journal, a group of authors summarized adjuvant mechanisms, characteristics and uses, seeks to overcome current vaccine limitations and provide valuable insights for future research and development. Examination: Vaccine adjuvants: mechanisms and platforms. Image credit: TelnovOleksii/Shutterstock.com Background Adjuvants are components…
exploring cutting-edge platforms for improved immunization
In a recent review published in the Signal Transduction and Targeted Therapy Journal, a group of authors summarized adjuvant mechanisms, characteristics, and applications, seeking to overcome current vaccine limitations and provide valuable insights for future research and development. Study: Vaccine adjuvants: mechanisms and platforms. Image Credit: TelnovOleksii/Shutterstock.com Background Adjuvants are components that…
Age-related self-DNA accumulation may accelerate arthritis in rats and in human rheumatoid arthritis
Statement of ethics and regulations Our clinical data collection was approved by the Research Ethics Committee of the Affiliated Hospital of Southwest Medical University (KY 2021010). The animal study was conducted according to the guidelines of the Institutional Animal Care and Use Committee of Macau University of Science and Technology…
IJMS | Free Full-Text | DNA Methylation in the Fields of Prenatal Diagnosis and Early Detection of Cancers
1. DNA Methylation: Looking Back over the Century “Genes are equivalent to blueprints; epigenetics is the contractor. They change the assembly, the structure.” —Bruce Lipton. The methylation of the DNA is a quintessential epigenetic mechanism consisting of the binding of a methyl group to the fifth carbon of the cytosine…
The effective treatment of malignant melanoma.
Plain-Language Summary Malignant melanoma (MM) is the most lethal skin cancer. Recent studies have found that the invasion and metastasis of MM are related not only to MM gene mutation but also to the interaction between MM cells and the immune system and microenvironment. The application of RNAi-based therapeutics to…
Cancers | Free Full-Text | A Novel Bispecific Antibody for EpCAM-Directed Inhibition of the CD73/Adenosine Immune Checkpoint in Ovarian Cancer
1. Introduction Typically, tumors in patients with ovarian cancer (OC) are often diagnosed at an advanced stage [1]. Currently, cytoreductive therapy with platinum-based chemotherapy is the mainstay of disease management, to which the majority of patients with OC initially respond. Unfortunately, 70% of these patients eventually develop refractory recurrences [2]….
Transcription and FACT facilitate the restoration of replication-coupled chromatin assembly defects
Struhl, K. & Segal, E. Determinants of nucleosome positioning. Nat. Struct. Mol. Biol. 20, 267–273 (2013). Article CAS PubMed PubMed Central Google Scholar Chereji, R. V. & Clark, D. J. Major determinants of nucleosome positioning. Biophys. J. 114, 2279–2289 (2018). Article ADS CAS PubMed PubMed Central Google Scholar Morgan, M….
Membrane recruitment of the polarity protein Scribble by the cell adhesion receptor TMIGD1
Buckley, C. E. & St Johnston, D. Apical-basal polarity and the control of epithelial form and function. Nat. Rev. Mol. Cell Biol. 23, 559–577 (2022). Article CAS PubMed Google Scholar Buchheit, C. L., Weigel, K. J. & Schafer, Z. T. Cancer cell survival during detachment from the ECM: multiple barriers…
Orla O’Sullivan on the impact of microbiome research
In the latest instalment of Creating the Future, Ann O’Dea speaks to Orla O’Sullivan, a leading authority in microbiome research with a background in bioinformatics. When a 17-year-old Orla O’Sullivan just missed out on the points to study medicine, she opted to study biological sciences with the intention of going…
From RNA to Protein – Molecular Biology of the Cell Ribosomal RNA (rRNA) Transfer RNA (tRNA) Messenger RNA (mRNA) Carries genetic information for protein tRNA synthesis mRNA and tRNA and rRNA Carries an amino acid at its 3′-end for mRNA and tRNA protein synthesis DmRNA and rRNA Is synthesized by RNA polymerase mRNA eukaryotes tRNA and rRNA Is synthesized by RNA polymerase Il in rRNA eukaryotes synthesized by RNA polymerase IIl in eukaryotes Synthesized by RNA polymerase in bacteria Self-folds into unique 3D conformation Catalyzes peptide bond formation
In the previously section we will watch that the final product of some genes is an RNA molecule even, such as those present in the snRNPs and in ribosomes. However, most genes in a cell herstellen mRNA molecules that serve as intermediaries on the pathway to protein. In this section…
CAR T cells as micropharmacies against solid cancers: Combining effector T-cell mediated cell death with vascular targeting in a one-step engineering process
General Lab Operation The assays were performed by experienced individuals throughout the course of the study. The study was performed using established laboratory protocols covering the processing, freezing, storage and thawing of cells as well as the staining procedure, data acquisition and gating strategy. Additional data beyond the data found…
What is PyTorch | IGI Global
Shortly You Will Be Redirected to Our Partner eContent Pro’s Website eContent Pro powers all IGI Global Author Services. From this website, you will be able to receive your 10% discount (automatically applied at checkout), receive a free quote, place an order, and retrieve your final documents. Note: The cost…
T cell infiltration into the brain triggers pulmonary dysfunction in murine Cryptococcus-associated IRIS
Ethics statement C57BL6, Rag1−/− (002216), Tcra−/− (002116), CCR5−/− (005427), Slc17a7-IRES2-Cre-D knock-in mice (Vglut1-cre, 023527), Cd4-cre (022071), and floxed EYFP (7903) mice purchased from The Jackson Laboratory were used for the current work. Mice were group-housed with two to five in an individual cage and kept under specific pathogen-free conditions with…
Whole genome sequencing and disease pattern in patients with juvenile polyposis syndrome: a nationwide study
This study is a nationwide study including all 66 Danish patients who fulfilled the clinical criteria of JPS and/or had a PV in SMAD4 or BMPR1A. We found that almost 90% of patients with a clinical diagnosis of JPS had a PV in either BMPR1A or SMAD4. Endoscopy had been…
Prioritization and functional validation of target genes from single-cell transcriptomics studies
List of congruent tip TEC markers The list of congruent tip TEC markers was extracted from a publicly available lung tumor study8. In brief, to identify congruent tip cell markers across freshly isolated human and murine TECs, as well as cultured human TECs, all genes that were most highly expressed…
Inhalable CAR-T cell-derived exosomes as paclitaxel carriers for treating lung cancer | Journal of Translational Medicine
Cells and plasmids HEK 293 T and LLC cells (ATCC) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Thermo Fisher Scientific) containing 10% FBS (Gibco, USA) and 1% penicillin–streptomycin (Invitrogen) at 37 °C in a 5% CO2 environment. Stbl3 and DH5α competent cells were used for plasmid transformation. The pCDH-CMV-MCS-EF1-GFP-Puro vector (cat….
SENP2 restrains the generation of pathogenic Th17 cells in mouse models of colitis
Mice Senp2f/f mice were described as previously16. T cell-specific Senp2 knockout mice (CKO, Senp2f/fLckCre+/+) and control mice (WT, LckCre+/+) in C57BL/6 genetic background were raised and kept in the Academia Sinica SPF animal facility, according to the rule of the Animal Protection Act of Taiwan. Lck-Cre mice, Rag2−/− mice and CD45.1…
Activation-inducible CAR expression enables precise control over engineered CAR T cell function
Primary cells PBMCs were either isolated from fresh buffy coats via density gradient centrifugation using Biocoll (Biochrom) or selected from a Leukapheresis material using in house T cell selection technology ATC. Buffy coats were obtained from autologous adult female or male blood donors at the Institute for Anesthesiology, German heart…
Difference between USCS exon coordinates and ensembl
Hello, I am trying to extract the coordinates for the exons in numerous genes. For example, APC using the MANE transcript: NM_000038.6 When downloading the USCS table browser for just exons from HG38 I get these coordinates for the exons (example exon 1): chr5 112737884 112737925 However, when I look…
Robust IL-2-dependent antitumor immunotherapy requires targeting the high-affinity IL-2R on tumor-specific CD8+ T cells
Introduction Interleukin (IL)-2 was the first Food and Drug Administration approved immunotherapy for use in patients with metastatic melanoma and renal cell carcinoma.1 2 However, the use of recombinant IL-2 (Proleukin/aldesleukin) has been problematic due to low response rates, off-target effects, and severe dose-dependent toxicities. Due to its potent T-cell…
von Willebrand disease drug gets FDA orphan drug designation
The U.S. Food and Drug Administration (FDA) has granted Vega Therapeutics, Inc. orphan drug designation for its antibody therapy, VGA039, for the treatment of the rare bleeding disorder, von Willebrand disease (VWD). Vega Therapeutics is a clinical stage biotechnology company developing novel therapies for rare blood disorders. VGA039 is a…
Which type of variant caller should I use in a WES normal cell line sample?
Which type of variant caller should I use in a WES normal cell line sample? 0 I have whole-exome sequencing data of an immortalised non-tumor (normal) cell line that I wish to assess for the presence/absence of APC/Wnt mutations. This is to double check that the cell line is sufficiently…
Epigenetic regulation of human-specific gene expression in the prefrontal cortex | BMC Biology
Preuss TM. The human brain: rewired and running hot. Ann N Y Acad Sci. 2011;1225 Suppl 1(Suppl 1):E182-191. Article PubMed Google Scholar Roth G, Dicke U. Evolution of the brain and intelligence in primates. Prog Brain Res. 2012;195:413–30. Article PubMed Google Scholar Laland K, Seed A. Understanding human cognitive uniqueness….
The roles and implications of RNA m6A modification in cancer
Huang, H., Weng, H. & Chen, J. m6A modification in coding and non-coding RNAs: roles and therapeutic implications in cancer. Cancer Cell 37, 270–288 (2020). Article CAS PubMed PubMed Central Google Scholar Qing, Y., Su, R. & Chen, J. RNA modifications in hematopoietic malignancies: a new research frontier. Blood 138,…
Deletion of endothelial leptin receptors in mice promotes diet-induced obesity
Experimental animals The generation of mice with tamoxifen-inducible, Tie2.Cre-ERT2-mediated deletion of LepR in endothelial cells was described previously12,17. For Cre recombinase activation, mice (6 weeks-of-age) were fed tamoxifen citrate-containing rodent chow (Envigo; TD.130860) for 6 weeks49. Genomic DNA from the brain, lung, small intestine, subcutaneous adipose tissue (SCAT) and visceral adipose tissue…
Cumulative effects of weakly repressive regulatory regions in the 3’ UTR maintain PD-1 expression homeostasis in mammals
The PD-1 3’ UTR repressed reporter gene expression by promoting mRNA decay The human PD-1 3’ UTR is made up of 1,174 nucleotides, longer than its coding region (867 nts). To explore the revolutionary relationship of PD-1 3’ UTRs of species, we systematically analyzed the sequence conservation of 21,050 protein-coding…
6 biotech companies in Norway you should know about
Best known for its folklore and tales of the Vikings, Norway’s landscape boasts postcard-like islands and the northern lights. In addition to ranking top as one of the happiest nations in the world, the country relies on sectors like oil and gas, hydropower and aquaculture to strengthen its economic growth….
Steps Taken for Equitable Access to Microbiology with Open Science
Microbes are everywhere and impact everyone, making it critical that microbiology research is equitably accessible to as many people and places as possible. However, inequitable access to research and publications has long plagued the science, technology, engineering and mathematics (STEM) enterprise-including the field of microbiology-due to paywalls that limit viewing…
Convergent evolution of SARS-CoV-2 Omicron subvariants leading to the emergence of BQ.1.1 variant
Ethics statement All experiments with hamsters were performed in accordance with the Science Council of Japan’s Guidelines for the Proper Conduct of Animal Experiments. The protocols were approved by the Institutional Animal Care and Use Committee of National University Corporation Hokkaido University (approval ID: 20-0123 and 20-0060). All protocols involving…
Targeting Poly(ADP)ribose polymerase in BCR/ABL1-positive cells
Cells and cell culture KOPN30, BV173, and K562 are BCR/ABL1-positive leukemia cell lines. All leukemia cell lines, as well as Ba/F3 cells, were maintained in RPMI-1640 medium supplemented with 15% fetal bovine serum (FBS) and penicillin–streptomycin (100 U/mL) at 37 °C in an atmosphere containing 5% CO2. KOPN30 cells were obtained…
Researchers complete first-ever mapping of human gut virus
Structure of the ΦcrAss001 virion and functional assignments. a,b, Electron micrographs of virions recorded using negative staining (a; n = 16 micrographs) and under cryogenic conditions (b; n = 44,006 micrographs). Scale bars, 100 nm. c, Molecular surface of virion reconstruction viewed from outside (left) and z-clipped (right), colored by gene product. DNA (gray) on…
APC researchers grow and map out a human gut virus
The researchers have learned more about this prominent gut virus by growing it in a lab, in the hopes of understanding how it impacts the human microbiome. Researchers at APC Microbiome Ireland have developed a detailed structural atlas of a prominent gut virus, to learn more about its role in…