Tag: ATG

Comparative mitochondrial genome brings insights to slight variation in gene proportion and large intergenic spacer and phylogenetic relationship of mudskipper species

Mitogenome organization, composition, and skewness The complete mitochondrial genome of B. dussumieri is (GenBank accession XX) 16,685 bp of length, which is similar to other mudskippers mitogenomes (16,470–17,243 bp) (Tables 1 and 2). The mitochondrial genome and the structure were also typical of the mudskipper species and with highly conservative sites, comprising…

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Domestic pigs are susceptible to experimental infection with non-human primate-derived Reston virus without the need for adaptation

Ethics and animal welfare statement All infectious work with RESTV, including sample inactivation, was performed in the Containment Level 4 laboratory (CL4) in accordance with the policies and protocols outlined by the Canadian Science Centre for Human and Animal Health Institutional Biosafety Committee. All animal work was performed in strict…

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Evidence of an intracellular creatine-sensing mechanism that modulates creatine biosynthesis via AGAT expression in human HAP1 cells

Cell lines and reagents HAP1 cells used to generate the reporter cells were grown from a frozen stock of HAP1 (C859) passage 6 purchased from Horizon Discovery LTD (Cambridge, UK). Iscove’s media (Ref: 098–150, Wisent) supplemented with 10% Fetal Bovine serum (Multicell) was used to grow HAP1 cells. All cells…

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How to run diamond blastp to get all vs all similarity score between proteins?

How to run diamond blastp to get all vs all similarity score between proteins? 0 Hi. I have a .fa file representing multiple predicted proteins. Example: >NC_001341.1_1 # 397 # 714 # 1 # ID=1_1;partial=00;start_type=ATG;rbs_motif=None;rbs_spacer=None;gc_cont=0.314 MCSSSIISEKHLKKNIFQKKAKVQYKIKKNRRGQINENKCSINPNKKRSKKIKKLAKQKD IQACINIGNRYVDVPIRPVSVADPDTPKETKEDKEKGCHFRNGIH* >NC_001341.1_2 # 788 # 1009 # 1 # ID=1_2;partial=00;start_type=ATG;rbs_motif=GGAG/GAGG;rbs_spacer=5-10bp;gc_cont=0.306 MQCLISNEYHHNNNEHTSCINRINRNYRSNQRHHQGYNDLYDSINIIQGMLENLNASIVY FTKDGKYKLIMTL* Given this…

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Thyroid hormone-regulated chromatin landscape and transcriptional sensitivity of the pituitary gland

Mouse genetic models The ThrbHAB allele expresses TRβ proteins (TRβ1 and TRβ2) fused to a peptide with a hemagglutinin (HAx2) tag and a site for biotinylation by prokaryotic BirA ligase, modified from a published tag30. The tag was inserted at the endogenous Thrb gene by homologous recombination in W9.5 (129/Sv)…

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Phenotypic profiling of solute carriers characterizes serine transport in cancer

Cell culture All cell lines used in this study were cultured at 37 °C in 5% CO2 in a humidified incubator. Human cell lines were authenticated by STR profiling using Promega GenePrint 10 and tested for Mycoplasma using Mycoalert (Lonza). Other than HCT116 p21−/− (a gift of B. Vogelstein60) all cell…

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The role of APOBEC3B in lung tumor evolution and targeted cancer therapy resistance

Cell line and growth assays Cell lines were grown in Roswell Park Memorial Institute-1640 medium (RPMI-1640) with 1% penicillin–streptomycin (10,000 U ml−1) and 10% FBS or in Iscove’s modified Dulbecco’s medium (IMDM) with 1% penicillin–streptomycin (10,000 U ml−1), l-glutamine (200 mM) and 10% FBS in a humidified incubator with 5% CO2 maintained at 37 °C. Drugs…

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Gene of choice – RefSeq Accession: NM_ cDNA NM_ ACATTTGCTT CTGACACAAC TGTGTTCACT AGCAACCTCA

Gene of choice: HBB (hemoglobin subunit beta) located in chromosome11:5,225,464 – 5,227,071 which contains 1,608bp. RefSeq Accession: NM_00518 cDNA NM_000518 ACATTTGCTT CTGACACAAC TGTGTTCACT AGCAACCTCA AACAGACACC 50 ATGGTGCATC TGACTCCTGA GGAGAAGTCT GCCGTTACTG CCCTGTGGGG 100 CAAGGTGAAC GTGGATGAAG TTGGTGGTGA GGCCCTGGGC AGGCTGCTGG 150 TGGTCTACCC TTGGACCCAG AGGTTCTTTG AGTCCTTTGG GGATCTGTCC 200 ACTCCTGATG CTGTTATGGG CAACCCTAAG GTGAAGGCTC ATGGCAAGAA 250…

Continue Reading Gene of choice – RefSeq Accession: NM_ cDNA NM_ ACATTTGCTT CTGACACAAC TGTGTTCACT AGCAACCTCA

The Rauvolfia tetraphylla genome suggests multiple distinct biosynthetic routes for yohimbane monoterpene indole alkaloids

Genome sequencing and assembly of R. tetraphylla After DNA extraction from young leaves and sequencing, the R. tetraphylla genome was first assembled into 1008 contigs with an N50 of 3.7 Mb. After haplotigs removal and a final pilon polishing, the 364,945,498 bp final assembly was distributed across 76 scaffolds with an N50…

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Solved Examine the DNA RFLP analysis shown below. Compare

Transcribed image text: Examine the DNA RFLP analysis shown below. Compare the three sample digestions (A,B, and C) to the Reference sample (Ref). Which sample matches the reference? ✓ Open in Reading View ATG A ATTCGTG △AATTCTCGCATTGTG AATTCTGTGTGATAAGACGAATTCTTAGTAG △A ATTCG TACTTAA GCACTTAAGAGCGTAACACTTAAGACACACTATTCTGCTTAAGAATCATCTTAAGC A B C D E F Figure 10.4:…

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Google Cloud helps Argility boost SA retail competitiveness

Marko Salic, CTO, The Argility Technology Group (ATG). Argility Technology Group, a Digicloud Africa Google Cloud partner, is helping local retailers transform their operations, grow margins and become more competitive using solutions built on the Google Cloud. Argility CTO Marko Salic says advanced digital technologies are key to enabling large…

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Significant Pathogenic Nontuberculous Mycobacteria | IDR

Introduction The Mycobacterium genus encompasses various species, including the Mycobacterium tuberculosis complex, which is responsible for tuberculosis (TB) infections, Mycobacterium leprae, which associated with leprosy, and a group of nontuberculous mycobacteria (NTM) species.1 The NTM species, also known as Mycobacteria other than tuberculosis (MOTT), can be widely found in the…

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How An Unexpected Discovery in a University Pond Changed the DNA Rulebook

Accidental discoveries have led to numerous breakthroughs and innovations throughout history; the antibiotic penicillin, the microwave and even the drug Viagra are all products of chance discoveries.  Serendipity in science often challenges laws or rules that we have previously regarded as established and absolute. It serves as a tool for…

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Mycobacterium tuberculosis Sub Lineage 4.2.2/SIT149 as DR

Introduction Antimicrobial resistance is a hidden global pandemic that shattered over 4.9 million people in 2019 alone, and the burden is highest, mainly in low-resource settings.1 Drug-resistant tuberculosis (DR-TB) caused by Mycobacterium tuberculosis (Mtb) complex (MTBC), which is resistant to one or more anti-TB drugs, is a leading global public…

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W2 Ex Genbank-new-answers – 22111 – ExGenbank-new-answers From 22111 Note: numbers in Part 2 and

ExGenbank-new-answers From 22111 Note: numbers in Part 2 and Part 3 are updated on February 7, 2022. Part 1 QUESTION 1.1 a) Inspecting the FEATURE table of the entry reveals that two CDS regions are defined; therefore there are two genes in this entry. As stated on the GenBank hand-out…

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Solved The Structure And Sequence Of The Human BetaGlobin

Transcribed image text: The Structure And Sequence Of The Human BetaGlobin Gene The sequence below is the sequence of the human beta-globin gene from chromosome 11 . This particular version of the beta-globin gene’s sequence is from the National Center for Biotechnology Information’s GenBank database. GenBank is the repository for…

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Routine test exposes distinct genetic code variation.

Newswise — Scientists testing a new method of sequencing single cells have unexpectedly changed our understanding of the rules of genetics.  The genome of a protist has revealed a seemingly unique divergence in the DNA code signalling the end of a gene, suggesting the need for further research to better…

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The Distinct Divergence in the Genetic Code

Oct 6 2023Reviewed by Danielle Ellis, B.Sc. A protist’s genome revealed an apparently unusual divergence in the DNA coding signaling the end of a gene, indicating the need for more research to better comprehend this varied group of creatures. In Oligohymenophorea sp. PL0344, only TGA functions as a stop codon….

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The end of genes: routine test reveals unique

Scientists testing a new method of sequencing single cells have unexpectedly changed our understanding of the rules of genetics.  The genome of a protist has revealed a seemingly unique divergence in the DNA code signalling the end of a gene, suggesting the need for further research to better understand this…

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Routine Test Discovers Unprecedented Divergence in Genes

Scientists testing a new method of sequencing single cells have unexpectedly changed our understanding of the rules of genetics. The genome of a protist has revealed a seemingly unique divergence in the DNA code signalling the end of a gene, suggesting the need for further research to better understand this…

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Comparative chloroplast genome analysis of four Polygonatum species insights into DNA barcoding, evolution, and phylogeny

Chloroplast genome features of four Polygonatum species In this phase of our study, we pursued the sequencing of four Polygonatum species namely P. filipes, P. cyrtonema, P. zanlanscianense and P. odoratum, using the Illumina HiSeq 2500 sequencing platform. This endeavour yielded a corpus of Illumina reads ranging from 15,915,998 to…

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ncRNA | Free Full-Text | Long Non-Coding RNA TUG1 Gene Polymorphism and TUG1 Expression Level as Molecular Biomarkers of Systemic Lupus Erythematosus and Lupus Nephritis

1. Introduction Systemic lupus erythematosus (SLE) is a chronic autoimmune disease with a wide variety of manifestations ranging from mild cutaneous to organ failure as lupus nephritis (LN) and cardiopulmonary complications [1]. SLE is mainly present among young women, with a greater incidence in certain ethnic groups, such as Asian,…

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Aedes aegypti Argonaute 2 controls arbovirus infection and host mortality

Generation and characterization of Ago2 knockout mutants To demonstrate the essential function of Ago2 in the Ae. aegypti siRNA pathway, we used CRISPR/Cas9 to knock out Ago2 and then investigated its role in defending against arbovirus infection (Fig. 1a). Ae. aegypti Ago2 (AeAgo2) has four exons and four predicated functional domains,…

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St. Jude Children’s Research Hospital hiring Principal/ lead/ Senior Bioinformatic Research Scientist Position in Cancer Genomics and/or Metabolomics in Memphis, Tennessee, United States

The Bioinformatics Research Scientist functions independently to coordinate large-scale bioinformatics projects, providing project oversight, scientific and technical advice, programming support, and other input as required to achieve established objectives. Coordinates the specialized expertise of bioinformatics colleagues to achieve scientific objectives. Establishes best practice recommendations, creates educational materials, and participates in…

Continue Reading St. Jude Children’s Research Hospital hiring Principal/ lead/ Senior Bioinformatic Research Scientist Position in Cancer Genomics and/or Metabolomics in Memphis, Tennessee, United States

Bioinformatic Research Scientist in Cancer Genomics and/or Metabolomics

Job Title: Bioinformatic Research Scientist in Cancer Genomics and/or Metabolomics Job Number: 65651 Location: Memphis, US Job Description A Bioinformatic Research Scientist position is available in the lab of Dr. Jian Xu at Department of Pathology and Center of Excellence for Leukemia Studies (CELS) at St. Jude Children’s Research Hospital to study…

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Eimeria zuernii (Eimeriidae: Coccidia): mitochondrial genome and genetic diversity in the Chinese yak | Parasites & Vectors

Eimeria zuernii mitogenome feature The E. zuernii linear mitogenome was 6176 bp in size and encoded three PCGs, cytb, cox1, and cox3, as well as seven interspersed small subunit (SSU) and twelve interspersed large subunit (LSU) rDNA fragments (Fig. 2). No transfer RNAs (tRNAs) were found in the E. zuernii mitogenome, similar…

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IJMS | Free Full-Text | The Impact of the CYP2D6 and CYP1A2 Gene Polymorphisms on Response to Duloxetine in Patients with Major Depression

Received: 16 July 2023 / Revised: 25 August 2023 / Accepted: 29 August 2023 / Published: 30 August 2023 Round 1 Reviewer 1 Report The manuscript “The impact of the CYP2D6 and CYP1A2 Gene Polymorphisms on Response to Duloxetine in Patients with Major Depression” addresses how some SNPs of these…

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Translation dysregulation in neurodegenerative diseases: a focus on ALS | Molecular Neurodegeneration

Tandan R, Bradley WG. Amyotrophic lateral sclerosis: part 1. Clinical features, pathology, and ethical issues in management. Annals of Neurology: Official Journal of the American Neurological Association and the Child Neurology Society. 1985;18:271–80. Article  CAS  Google Scholar  Mehta P et al. Prevalence of amyotrophic lateral sclerosis (ALS), United States, 2016….

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Use of bioinformatics in genome research in Saudi Arabia

Background Bioinformatics is a new discipline that merges biology, computer science, and information technology. It involves developing and integrating techniques, such as applied mathematics, statistics, computer science, chemistry, and biochemistry, to solve biological problems. Bioinformatics researchers develop and use computing tools to gain more about the life and survival of…

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10 Plus AI Research Projects Everyone Should be Aware Of

AI has been making remarkable progress, revolutionizing numerous industries and capturing the imagination of experts and enthusiasts worldwide. Several notable research projects have emerged, showcasing the immense potential of artificial intelligence. By examining these projects, we can gain insights into the transformative impact of AI on various sectors. One prominent…

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Age-related self-DNA accumulation may accelerate arthritis in rats and in human rheumatoid arthritis

Statement of ethics and regulations Our clinical data collection was approved by the Research Ethics Committee of the Affiliated Hospital of Southwest Medical University (KY 2021010). The animal study was conducted according to the guidelines of the Institutional Animal Care and Use Committee of Macau University of Science and Technology…

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Google launches Bard (and it fails the translation test :)

Blog:Google launches Bard (and it fails the translation test 🙂 1 Google has launched Bard in the EU. Just for fun, I tested its basic molecular biology skills in the same way as with ChatGPT previously. I think you get the idea. ChatGPT can do this in the meantime (after…

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Drug resistance-associated mutations of M.TB

Introduction Tuberculosis (TB) is one of the most common causes of death and the leading cause of a single infectious agent.1 According to the 2022 Global TB Report of the World Health Organization (WHO), 10.6 million people were infected with TB in 2021, and although the annual number of deaths…

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Solved The start of the lung gene is shown above with the

The start of the lung gene is shown above with the start ATG codon at the beginning. You have two potential crRNA that might target this sequence. Which one of the two would work to inactivate this gene? Explain. (Red is used to show the PAM sequence. Review how guide…

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A viral pan-end RNA element and host complex define a SARS-CoV-2 regulon

Cell lines and culture A549-hACE2 [a gift from Dr. Ben tenOever3], 293 T, HEK293, A549, and 3T3-L1 pre-adipocyte cell lines (refer to Supplementary Table 1) were cultured in DMEM and 10% FBS. U937, Calu-3, and HCT116 cells were cultured in RPMI, EMEM, and McCoy’s 5 A media, respectively, with 10% FBS. Caco-2 cells…

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Corrigendum: Crosstalk between cGAS-STING pathway and autophagy in cancer immunity

Published Erratum . 2023 May 19;14:1216456. doi: 10.3389/fimmu.2023.1216456. eCollection 2023. Affiliations Expand Affiliations 1 Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China. 2 Cancer Institute, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China. 3 Department of Laboratory Medicine, Huadong Hospital, Fudan University, Shanghai,…

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Comparative mitogenome analyses of twelve non-biting flies and provide insights into the phylogeny of Chironomidae (Diptera: Culicomorpha)

General structure, organization, and composition of mitogenomes The gene order and arrangement characteristics of the twelve mitogenomes in the present study (Chironomus kiiensis, Chironomus nipponensis, Chironomus plumosus, Dicrotendipes pelochloris, Dicrotendipes sp., Einfeldia sp., Glyptotendipes tokunagai, Limnophyes sp., Nilodorum tainanus, Polypedilum nubifer, Smittia aterrima, and Tanytarsus formosanus) were similar to those…

Continue Reading Comparative mitogenome analyses of twelve non-biting flies and provide insights into the phylogeny of Chironomidae (Diptera: Culicomorpha)

Histone modifications regulate pioneer transcription factor cooperativity

Protein expression, mutagenesis and purification Xenopus laevis histones for nucleosome assembly were overexpressed in the Escherichia coli BL21(DE3) pLysS strain and purified from inclusion body as previously described48. The cells were grown in LB medium at 37 °C and induced with 1 mM IPTG when OD600 reached 0.6. After 3 h of expression,…

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Deletion of endothelial leptin receptors in mice promotes diet-induced obesity

Experimental animals The generation of mice with tamoxifen-inducible, Tie2.Cre-ERT2-mediated deletion of LepR in endothelial cells was described previously12,17. For Cre recombinase activation, mice (6 weeks-of-age) were fed tamoxifen citrate-containing rodent chow (Envigo; TD.130860) for 6 weeks49. Genomic DNA from the brain, lung, small intestine, subcutaneous adipose tissue (SCAT) and visceral adipose tissue…

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Mitogenomic phylogenetic analyses provide novel insights into the taxonomic problems of several hesperiid taxa (Lepidoptera: Hesperiidae)

Mitogenome features Nine new mitogenomes were assembled and annotated. Their circular maps were similar, thus only the map of Abraximorpha esta is shown (Fig. 1, Supplementary Fig. S1). The length of the mitogenomes ranged from the shortest, 15,284 bp (Coladenia maeniata) to the longest, 15,853 bp (Lotongus saralus chinensis and Trapezites iacchus). Each…

Continue Reading Mitogenomic phylogenetic analyses provide novel insights into the taxonomic problems of several hesperiid taxa (Lepidoptera: Hesperiidae)

Discovery of two new isoforms of the human DUT gene

Overview of the isoform-specific determination of dUTPase gene expression Our aim was to determine the mRNA expression level of the dUTPase isoforms specifically. First, we investigated the Ensemble, the UniProt, and the NCBI Reference Sequences (RefSeq) databases and also took into account the Consensus CDS (CCDS) project. In the Ensemble…

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Tareef Kawaf – President at RStudio

About Tareef Kawaf is the current president at RStudio. Tareef has over 25 years of experience in the technology industry, and has held various leadership positions at Brightcove, Inc., Ruckus Network, ATG, and Digital Equipment Corporation. Kawaf was responsible for recruiting and mentoring a world-class engineering team at Brightcove, Inc.,…

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The Dictyostelium discoideum genome lacks significant DNA methylation and uncovers palindromic sequences as a source of false positives in bisulfite sequencing

. 2023 Apr 18;5(2):lqad035. doi: 10.1093/nargab/lqad035. eCollection 2023 Jun. Affiliations Expand Affiliations 1 Biology Department, Clark University, 950 Main Street, Worcester, MA 01610, USA. 2 Howard Hughes Medical Institute and Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA. Free PMC article Item in Clipboard Robert A Drewell et…

Continue Reading The Dictyostelium discoideum genome lacks significant DNA methylation and uncovers palindromic sequences as a source of false positives in bisulfite sequencing

Spontaneous tumor regression mediated by human T cells in a humanized immune system mouse model

Human CD34+ cell isolation Human fetal liver (FL) samples were obtained from Advanced Biosciences Resources (Alameda, CA) with proper consent. FL samples were cut in small fragments, treated for 25 min at 37 °C with Collagenase D (100 ng/mL; Roche). The cell suspension was prepared, and the human CD34+ cells were separated by…

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The Give | Genexus System Next-Generation Sequencing

Faster, more accurate results. In less time. “The tests we perform here in our lab are very complex,” said Dr. Doha Itani, Division Head of the Molecular Diagnostics and Cytogenetics Laboratory at Horizon’s Saint John Regional Hospital. “These complicated tests can require a lot of hands-on time.” Dr. Doha Itani,…

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Solved Which two of the following statements about

Transcribed image text: Which two of the following statements about transcription are FALSE? Genes transcribed in the same direction use the same template strand. The direction of transcription is determined by the promoter sequence. A transcript is elongated by adding new nucleotides to its 5′ end. The start site for…

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tool that reports *every* ORF, including from nested starts?

tool that reports *every* ORF, including from nested starts? 2 (I am defining ORF as, between an ATG start and a downstream stop codon) Given a situation like this: showing a ~1kb region, forward strand, 3 frame prediction of starts (green) and stops (red), for the frame 3 span between…

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Solved 4. Use the two gene sequences to answer the rest of

4. Use the two gene sequences to answer the rest of the questions: 121 aactt ATG gat aac aat ccg aac atc aat gaa tga att cct tat aat tgt tta agt aac c (mistake sequence) 121 aactt atg gat aac aat ccg aac atc aat gaa tgc att cct…

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Write a function called get_genes(dna_string) that takes in a string of uppercase letters similar to the above, and returns a list of strings representing the genes in that DNA sequence.

Write a function called get_genes(dna_string) that takes in a string of uppercase letters similar to the above, and returns a list of strings representing the genes in that DNA sequence. The rules for how genes work are complex, but we’re going to make some simplifying assumptions:  Individual genes are substrings…

Continue Reading Write a function called get_genes(dna_string) that takes in a string of uppercase letters similar to the above, and returns a list of strings representing the genes in that DNA sequence.

Confused on strandedness, what are antisense transcripts?

Confused on strandedness, what are antisense transcripts? 1 Hello, I am trying to wrap my head around anti-sense transcripts. When looking at refseq’s annotations on IGV you can see a (-) or a (+) for each transcript. From reading this paper www.ncbi.nlm.nih.gov/pmc/articles/PMC3664467/#sec-2title I understand that transcription from the antisense strand…

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The Biostar Herald for Monday, March 13, 2023

The Biostar Herald publishes user submitted links of bioinformatics relevance. It aims to provide a summary of interesting and relevant information you may have missed. You too can submit links here. This edition of the Herald was brought to you by contribution from Istvan Albert, and was edited by Istvan…

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A male-killing gene encoded by a symbiotic virus of Drosophila

Collection and maintenance of Drosophila biauraria We used laboratory stocks of Drosophila biauraria (Diptera; Drosophilidae), which were originally collected at the Field Science Center for Northern Biosphere, Hokkaido University located at Tomakomai, Hokkaido in 2011 and 2015 using standard banana traps and sweeping22. Females were brought into the lab and…

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Solved Part II- Short answer (point value is indicated in

Transcribed image text: Part II- Short answer (point value is indicated in parentheses) 21. (15) This diagram represents regions around a gene encoding a mRNA. Exon I (labeled CE) of the mRNA contains the translation initiation ATG codon (labelled D). Exon IV (labeled JL) contains the TGA stop codon (labelled…

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Solved Examine the following DNA sequence:

Examine the following DNA sequence: 5’-AATGCGGGCAGTAGGCCCGTTTATACCAAAAATGACAGAAATGGGCCAT-3’ A. Write the reverse complement of this sequence in 5’ to 3’ direction (1 point) B. The open reading frame (ORF) of the sequence starts with the first ATG and in red is a sgRNA target sequence):….ATGCGGGCAGTAGGCCCGTTTATACCAAAAATGACAGAAATGGGCTTGATC…. i. What is the protospacer sequence of…

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Crosstalk between cGAS-STING pathway and autophagy in cancer immunity

1 Introduction Cancer is one of the world’s most serious threats to human health, with high morbidity and mortality rates, and according to the latest global data, 9.96 million patients will die from cancer in 2020 (1). Cancer is a genetic abnormal disease triggered by a long-term combination of multiple…

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Definition of nucleotide in Microbiology.

Examples of nucleotide in the following topics: Nucleotide and Nonnucleotide Reverse Transcriptase Inhibitors They mimic, respectively, nucleosides or nucleotides but lack a free hydroxyl group at the 3′ end. Nucleoside and nucleotide inhibitors are also called competitive substrate inhibitors. Non-nucleotide inhibitors are non-competitive inhibitorsof reverse transcriptase. The mechanisms for resistance…

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CvkR is a MerR-type transcriptional repressor of class 2 type V-K CRISPR-associated transposase systems

Architecture of cyanobacterial CAST systems Starting from the known CAST components, we searched for conserved genes and genetic elements in their vicinity. These elements included the left and right ends of the transposon, the neighboring tRNA, CRISPR arrays and tracrRNA, the transposase genes (tnsB, tnsC and tniQ), and genes in…

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Solved How do I properly find an Open Reading Frame, start

How do I properly find an Open Reading Frame, start location for “ATG” codon, stop codon location for that reading frame, and length of the sequence in Python? (This is for Computational Biology) If i run the length code separately, it gives me the length of the sequence in question….

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rRNA, tRNA and mRNA – learn & understand it online

Your cells contain a vast amount of cells that each have blueprints in them with instructions for creating proteins necessary to maintain homeostasis. Your body undergoes multiple biochemical and physiological pathways each day in order for you to live life without any complications. Proteins mediate these biochemical reactions. Proteins are…

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Geneseeq receives CE Marks for MRD and MCED tests

TORONTO, Jan. 24, 2023 /PRNewswire/ -Geneseeq Technology Inc. announced that Geneseeq’s multi-cancer minimal residual disease detection (MRD) and multi-cancer early detection (MCED) kits were both CE Marked for liquid biopsy use in solid tumor patients. Geneseeq Logo (CNW Group/Geneseeq Technology Inc.) The markings will enable Geneseeq to expand its global…

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Solved The anticodon binds to a complementary codon in the

Transcribed image text: The anticodon binds to a complementary codon in the molecule. tRNA mRNA DNA rRNA QUESTION 2 The complementary anticodon to UAC is TAG ATG AUG UAC AUG UAC QUESTION 3 Amino acid-accepting end and anticodon are typically found in mRNA DNA IRNA rRNA QUESTION 4 The role…

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Aonchotheca (Nematoda: Capillariidae) is validated as a separated genus from Capillaria by both mitochondrial and nuclear ribosomal DNA | Parasites & Vectors

First molecular identification of A. putorii in China The mt cox1 sequence of PCR amplicon of 652 bp (GenBank accession no: OP363931) using primer pair JB3-JB4.5 as previously described [21] showed only 81.7% identities to GenBank no. MH665361 by BLAST search, which was a Capillaria sp. This low identity indicates the…

Continue Reading Aonchotheca (Nematoda: Capillariidae) is validated as a separated genus from Capillaria by both mitochondrial and nuclear ribosomal DNA | Parasites & Vectors

Antarctic aldehyde dehydrogenase from Flavobacterium PL002 as a potent catalyst for acetaldehyde determination in wine

Sequence analysis of S2-ALDH BLAST screening of the Flavobacterium PL002 genome sequence42 led to identification of a coding region homologous to aldehyde dehydrogenase gene (1365 bp) coding for the hypothetical enzyme S2-ALDH of 454 amino acids, with calculated MW 49,062 (Supplementary Fig. 1S). Phylogenetic analysis of the S2-ALDH amino acid sequence in…

Continue Reading Antarctic aldehyde dehydrogenase from Flavobacterium PL002 as a potent catalyst for acetaldehyde determination in wine

How to read the AUGUSTUS results

Hi, everyone. I used AUGUSTUS to make a genetic prediction for a certain fish, but I don’t know how to read the results. I mainly do not understand the following three points. 1.What does “sequence number” mean? How is it different from scaffold number? 2.What does “none” mean? Does it…

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Which is a stop codon?

A stop codon is a sequence of three nucleotides (a trinucleotide) in DNA or messenger RNA (mRNA) that signals a halt to protein synthesis in the cell. There are 64 different trinucleotide codons: 61 specify amino acids and 3 are stop codons (i.e., UAA, UAG and UGA). What are the…

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Dynamic mechanisms of CRISPR interference by Escherichia coli CRISPR-Cas3

In vitro reconstitution of Escherichia coli CRISPR-Cas3 interference E. coli CRISPR-Cas3 is generally well-characterized type I CRISPR complexes in vitro and in vivo32,33,37,38. However, recombinant EcoCas3 protein is difficult to purify because of poor solubility and propensity to aggregate at 37 °C25,26,30,39. Co-expression of HtpG chaperon40 and/or low temperature growth at…

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Using RFLP-PCR, Mini Sequencing and STR Techniques in Preimplantation Diagnosis of Spinal Muscular Dystrophy in Vietnam

Background: Spinal Muscular Atrophy (SMA) is one of the most common autosomal recessive disorders in children after Duchenne muscular dystrophy affecting approximately 1 in 11,000 live births. There is no effective therapy for SMA; however, broadening our knowledge about the molecular genetics of the disease is resultant to develop potential…

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Frontiers | A Mutated Nme1Cas9 Is a Functional Alternative RNase to Both LwaCas13a and RfxCas13d in the Yeast S. cerevisiae

Introduction The clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated (Cas) protein systems naturally exist in prokaryotes. They are RNA-mediated defense mechanisms to protect bacteria and archaea from invading nucleic acids (Barrangou et al., 2007). CRISPR–Cas systems are classified into two classes (1 and 2). Class 2 CRISPR–Cas require a single…

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Binding of the HSF-1 DNA-binding domain to multimeric C. elegans consensus HSEs is guided by cooperative interactions

The heat-shock response is represented by a small set of genes in C. elegans We initially aimed at identifying those HSE-containing promoters that are most strongly upregulated under heat-stress conditions. Given that the HSR is complex in nematodes we used data from several heat-shock studies based on microarray and RNAseq…

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CALITHERA BIOSCIENCES, INC. MANAGEMENT’S DISCUSSION AND ANALYSIS OF FINANCIAL CONDITION AND RESULTS OF OPERATIONS (form 10-Q)

You should read the following discussion and analysis of our financial condition and results of operations in conjunction with our unaudited condensed consolidated financial statements and related notes included in Part I, Item 1 of this report. This Quarterly Report on Form 10-Q contains forward-looking statements within the meaning of…

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Generation of Hook Ischemia-Reperfusion Model using a Three-Day Developing Chick Embryo

This paper describes ischemia-reperfusion (I/R) modeling in a 3-day chick embryo using a spinal needle customized hook to better understand I/R development and treatment. This model is simple, quick, and inexpensive. Hi, I am Dr.Syed Shadab Raza, the principal investigator for the Laboratory for Stem Cells and Restorative Neurology, located…

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Profiling and functional characterization of maternal mRNA translation during mouse maternal-to-zygotic transition

INTRODUCTION Mammalian life starts with the fusion of two terminally differentiated gametes, sperm and oocyte, resulting in a totipotent zygote. After going through preimplantation development, the zygote reaches blastocyst before implantation. The two most important events taking place during preimplantation development are zygotic genome activation (ZGA) and the first cell…

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Complete mitochondrial genome sequencing of Oxycarenus laetus (Hemiptera: Lygaeidae) from two geographically distinct regions of India

Genome organization and structure of O. laetus The MiSeq data of BTI and CBE samples of O. laetus was submitted to the NCBI SRA database under the accession numbers SRR11024516 and SRR11024517, respectively. The study was done to understand whether there exists a different species of the genus Oxycarenus similar…

Continue Reading Complete mitochondrial genome sequencing of Oxycarenus laetus (Hemiptera: Lygaeidae) from two geographically distinct regions of India

High tumor mutation burden and DNA repair gene mutations

Introduction Anaplastic lymphoma kinase (ALK)‑fusion genes represent a small but important part of oncogenic driver mutations in NSCLC, accounting for approximately 3%‑7% of all cases worldwide.1,2 Small molecule tyrosine kinase inhibitors (TKIs) are the standard therapy for ALK-rearranged NSCLC. Crizotinib, a first-generation TKI, is the most widely used targeted drug…

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mitochondrial dna damage triggers an ifn mediated immune response

2.6 Mitochondrial Damage-Induced Mitochondrial DNA Release is Central to Chronic Kidney Disease-Induced Type-I-Interferon Response in Vascular Smooth Muscle Cells DNA released from nuclear or mitochondria is the main source of endogenous DNA that activates cGAS-STING pathway. This is thought to be mediated by the presence 75 of leaked mitochondrial DNA…

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Prevalence and Molecular Characteristics Based on Whole Genome Sequenc

Introduction Tuberculosis, caused by Mycobacterium tuberculosis, remains one of the top 10 causes of death worldwide and the leading cause of death from a single infectious agent (ranking above HIV/AIDS).1 In 2020, World Health Organization (WHO) reported that 7.1 million people with tuberculosis were newly diagnosed and notified in 2019,…

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