Tag: BAC

Failure to detect mutations in U2AF1 due to changes in the GRCh38 reference sequence

Materials and Methods Genomic data was collected as part of the MDS National History Study or The Cancer Genome Atlas project and consented appropriately under those protocols 8 Sekeres M.A. Gore S.D. Stablein D.M. DiFronzo N. Abel G.A. DeZern A.E. Troy J.D. Rollison D.E. Thomas J.W. Waclawiw M.A. Liu J.J….

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Running SortMeRNA on Multiple Files

Running SortMeRNA on Multiple Files 0 Hi all, I am VERY new to SortMeRNA (I’m a PhD student taking a bioinformatics class that has been very poorly taught). I have 27 paired samples for a total of 54 samples named like this: SRR13711719_1_val_1.fq SRR13711719_2_val_2.fq. So the format is _1_val_1.fq and…

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callum-jpg/motifSearch: Find the occurrence of a specific motif in a genome

motifSearch is a python package intended for the end-to-end analysis of motif frequency within genomic DNA. This package offers a means for downloading, measuring motif frequency, and visualising the resulting data. Example plots Installation guide with linux # Clone the repo git clone github.com/callum-jpg/motifSearch.git # Change to the motifsearch directory…

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Shotgun sequencing method explained, 11.51 MB, 08:23

Your browser does not support the audio element Download lagu Shotgun sequencing method explained You can download MP3 for free at Free MP3 & Lyrics Download. Song details Shotgun sequencing method explained you can see in the table, for the download link Shotgun sequencing method explained below. Title Shotgun sequencing…

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What is the difference between GRCh37 and hs37? And hg19?

This is what I have found so far. Please correct me if I am wrong. GRCh37 w/o patches includes the primary assembly (22 autosomal, X. Y, and non-chromosomal supecontigs) and alternate scaffolds, but not a reference mitogenome. Non-chromosomal supercontigs are the unlocalized and unplaced scaffolds. The rCRS reference mitogenome in…

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Electroporation ELECTROPORATION It consists of applying short high voltage

ELECTROPORATION It consists of applying short high voltage pulses to a mixture of host cells in suspension and recombinant vectors in solution. These pulses increase the permeability of the membranes, allowing the vectors to pass into the cell. It is a highly efficient method that can be applied to all…

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