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Tag: BALB
Endogenous Coriobacteriaceae enriched by a high-fat diet promotes colorectal tumorigenesis through the CPT1A-ERK axis
Bacteria Strain Cori.ST1911 was isolated from fresh stool of 20-week-old C57/BL6J mice fed a HFD at the Animal Center of the West China Hospital of Sichuan University. Briefly, faecal particles were ground with a glass grinding rod and suspended in sterile phosphate-buffered saline (PBS). After gradient dilution, the suspension was…
csi-miR-96-5p delivered by Clonorchis sinensis extracellular vesicles promotes intrahepatic cholangiocarcinoma proliferation and migration via the ferroptosis-related PTEN/SLC7A11/GPX4 axis | Parasites & Vectors
Human tissue samples The preoperative diagnosis of CS mainly relies on stool microscopy, enzyme-linked immunosorbent assay, or endoscopy. Fresh liver and intrahepatic bile duct specimens were collected from ICC patients with (CS-ICC group) and without (NC-ICC group) CS infection during partial hepatectomy at the First Hospital of Jilin University. Tumor tissues…
Dihydroquercetin (DHQ) ameliorates LPS-induced acute lung injury by regulating macrophage M2 polarization through IRF4/miR-132-3p/FBXW7 axis
Background: Acute lung injury (ALI) is a common complication of sepsis. Dihydroquercetin (DHQ) has been found to attenuate lipopolysaccharide (LPS)-induced inflammation. However, the effect of DHQ on LPS-challenged ALI remains unclear. Methods: Pulmonary HE and TUNEL staining and lung wet/dry ratio were detected in LPS-treated Balb/c mice. IL-1β, IL-6 and…
SOCS2 inhibits hepatoblastoma metastasis via downregulation of the JAK2/STAT5 signal pathway
Weighted gene co-expression network analysis GSE131329 has comprehensive clinical data, and WGCNA was carried out with this expression profile. WGCNA is a systems biology method for characterizing gene association patterns between different samples, used to identify highly synergistic sets of genes and to screen for candidate biomarker genes or therapeutic…
A natural product YSK-A blocks SARS-CoV-2 propagation by targeting multiple host genes
Hoareau, L. & DaSilva, E. J. Medicinal plants: A re-emerging health aid. Electron. J. Biotechnol. 2, 3–4 (1999). Google Scholar Jahan, I. & Ahmet, O. Potentials of plant-based substance to inhabit and probable cure for the COVID-19. Turk. J. Biol. 44, 228 (2020). Article CAS PubMed PubMed Central Google Scholar …
Mouse Brain Tissue Preparation 8: Normal Nerve Astrocytes | Technique alternative | 01018463296
About Mouse Brain Tissue Preparation Buffer 8: Normal Nerve Astrocytes Check information Catalog number: 9-80131 Full name: Mouse Brain Tissue Preparation Buffer 8: Normal Nerve Astrocytes Size: 1 x 100 ml Supplier: CHI Scientific Price: 207.00 Shelf life : The shelf life of this product is: 6 mois Storage : The storage of this product is…
Immune-privileged tissues formed from immunologically cloaked mouse embryonic stem cells survive long term in allogeneic hosts
Mice C57BL/6N (strain 005304), C3H/HeJ (strain 000659), FVB/NJ (strain 001800), BALB/cJ (strain 000651) and NSG mice (stock 005557) were purchased from the Jackson Laboratory. CD-1 (stock 022) mice were purchased from Charles River. Mice (6–20-week-old) of each strain/background were used for teratoma assays. Mice were housed in a pathogen-free facility…
SNPs of a specific mouse strain
Hi, I wonder how can I get SNPs for a particular mouse strain like C57BL6. I have downloaded a mouse reference vcf from ftp.ebi.ac.uk/pub/databases/mousegenomes/REL-2112-v8-SNPs_Indels/mgp_REL2021_snps.rsID.vcf.gz Its header is #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT 129P2_OlaHsd 129S1_SvImJ 129S5SvEvBrd A_J AKR_J B10.RIII BALB_cByJ BALB_cJ BTBR_T+_Itpr3tf_J BUB_BnC3H_HeH C3H_HeJ C57BL_10J C57BL_10SnJ C57BL_6NJ…
31L-APC-100ul | Interleukin 2 Receptor, alpha (IL-2Ra, IL-2R alpha)
Cat# I7663-31L-APC-100ul Size : 100ul Brand : US Biological Host mouse Conjugate APC Isotype IgG1 Clone Number 10B1132 (IL-A111) Grade Affinity Purified Applications IHC IP Shipping Temp Blue Ice Storage Temp 4°C Do Not Freeze Applications: Suitable for use in Immunohistochemistry, Immunoprecipitation and Functional Assays. Other applications not tested. Recommended…
31L-AP-100ul | Interleukin 2 Receptor, alpha (IL-2Ra, IL-2R alpha)
Cat# I7663-31L-AP-100ul Size : 100ul Brand : US Biological Host mouse Conjugate AP Isotype IgG1 Clone Number 10B1132 (IL-A111) Grade Affinity Purified Applications IHC IP Shipping Temp Blue Ice Storage Temp 4°C Do Not Freeze Applications: Suitable for use in Immunohistochemistry, Immunoprecipitation and Functional Assays. Other applications not tested. Recommended…
Biological and genetic characterization of a newly established human external auditory canal carcinoma cell line, SCEACono2
Ethic statement The Clinical Research Ethics Review Committee of Kyushu University Hospital approved the study (permit no. 29-43, 30-268, and 700-00). Written informed consent for the current research project was obtained before the tumor tissue, and a blood sample were harvested. This study was also conducted according to the principles…
Advancements in Rift Valley fever vaccines: a historical overview and prospects for next generation candidates
Gerken, K. N. et al. Paving the way for human vaccination against Rift Valley fever virus: a systematic literature review of RVFV epidemiology from 1999 to 2021. PLoS Negl. Trop. Dis. 16, e0009852 (2022). Article CAS PubMed PubMed Central Google Scholar Pepin, M., Bouloy, M., Bird, B. H., Kemp, A….
Both chimpanzee adenovirus-vectored and DNA vaccines induced long-term immunity against Nipah virus infection
Ethics statement The animal experiments were approved by the Animal Ethics Committee of the Wuhan Institute of Virology, Chinese Academy of Sciences (approval number: WIVA21202104). All animal experiments involving NiV were executed in the animal biosafety level 4 (ABSL-4) facility at the National Biosafety Laboratory (Wuhan), Chinese Academy of Sciences….
B4GALT5 shRNA Plasmid Mouse) – Gentaur.com
Catalog number MBS8274761 Price Please ask Size 0,15 mg Other size please contact us to order other different size Test Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40…
Neuron Navigator 1 (Nav1) regulates the response to cocaine in mice
Mouse strains All mouse procedures were approved by the Institutional Animal Care and Use Committees at Binghamton or Stanford University; and were conducted in accordance with the National Institute of Health Guide for Care and Use of Laboratory Animals, Eighth Edition. All mice were originally obtained from Jackson Laboratories, and…
DNA damage induced by CDK4 and CDK6 blockade triggers anti-tumor immune responses through cGAS-STING pathway
Patients’ tumor tissues and clinical data This study recruited 125 breast cancer patients for immunohistochemical (IHC) analysis and 10 breast cancer patients for RNA-seq analysis from the Affiliated Tumor Hospital of Nantong University. Tumor tissues collected from surgical patients were fixed with formalin and embedded with paraffin and then examined….
Molecular characterization and functional implications on mouse peripheral blood mononuclear cells of annexin proteins from Echinococcus granulosus sensu lato | Parasites & Vectors
Parasites and animals PSCs were collected from hydatid cysts isolated from the liver of sheep infected with E. granulosus s.l. from a slaughterhouse in Sichuan province, China. The PSCs and cyst walls were separated and treated as described in previous reports [22]. The viability and molecular genotyping of PSCs were…
The potential use of bacteria and bacterial derivatives as drug delivery systems for viral infection | Virology Journal
Novel approaches to treating viral illnesses must be developed. Drug delivery methods based on nanomaterials are attractive options in the search for therapeutic advantages because of their distinctive properties. Researchers should also examine how the most prevalent viruses, such as the flu, HIV, SARS-CoV-2, monkeypox, and HPVs, are being treated…
ncRNA | Free Full-Text | The Intergenic Type lncRNA (LINC RNA) Faces in Cancer with In Silico Scope and a Directed Lens to LINC00511: A Step toward ncRNA Precision
CRC Upregulated 153-5p HIF-1 activates LINC00511, targeting HIF-1’s 3-UTR and +ve feedback loop [78] Oncogene Human CRC tissues vs. paired adjacent non-tumor and CRC cell lines HT29, LOVO, SW620, SW480 vs. normal colon epithelial FHC cell line and athymic BALB/c nude mice for implantation – HNF4 promotes LINC00511 transcription, interaction…
A computationally designed antigen eliciting broad humoral responses against SARS-CoV-2 and related sarbecoviruses
Phylogenetic analysis Protein sequences of spike proteins were downloaded from the NCBI virus database for all the known sarbecoviruses (June 2020). A multiple sequence alignment was generated using MUSCLE36. The resulting multiple sequence alignment was pruned to the RBD region, filtered at 95% sequence identity and used as input for…
Schistosoma japonicum EKLF/KLF1 is a potential immune target to tackle schistosomiasis | Parasites & Vectors
Animals and parasites Snails infected with S. japonicum (Oncomelania hupensis) were provided by the Jiangxi Provincial Institute of Parasitic Diseases, Jiangxi, China. Freshly released cercariae stimulated by light were immediately harvested. Six-week-old pathogen-free male BALB/c mice and New Zealand white rabbits (both from Vital River Laboratory Animal Technology Co., Beijing,…
circRNA landscape in dorsal root ganglia from mice with collagen antibody-induced arthritis
Circular RNAs are a novel class of RNA molecules that are covalently closed into a ring structure. They are an epigenetic regulatory mechanism, and their best-studied function is regulation of microRNA activity. As such circular RNAs may be involved in the switch from acute to chronic pain. They have previously…
MCMBP shRNA Plasmid Mouse) – Gentaur.com
Catalog number MBS8280724 Price Please ask Size 0,15 mg Other size please contact us to order other different size Test Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40…
Conjugation of microbial-derived gold nanoparticles to different types of nucleic acids: evaluation of transfection efficiency
AuNP production Fusarium oxysporum was used for the extracellular production of AuNP. The fungus was acquired from the Culture Collection of Fungi of the Charles University (CCF 3732, Czech Republic). After cultivation in Sabouraud Dextrose Broth (SDB, Sigma Aldrich, Prague, Czech Republic) at 30 °C and 150 rpm for 4 days, the supernatant…
Effects of host species on microbiota composition in Phlebotomus and Lutzomyia sand flies | Parasites & Vectors
Maroli M, Feliciangeli MD, Bichaud L, Charrel RN, Gradoni L. Phlebotomine sandflies and the spreading of leishmaniases and other diseases of public health concern. Med Vet Entomol. 2013;27:123–47. CAS PubMed Google Scholar Akhoundi M, Kuhls K, Cannet A, Votypka J, Marty P, Delaunay P, et al. Historical overview of the…
Silencing of FCRLB by shRNA ameliorates MuSK-induced EAMG in mice,Journal of Neuroimmunology
Introduction Muscle specific kinase (MuSK) antibody positive myasthenia gravis (MG) often presents with a severe disease course and resistance to treatment. Treatment-refractory patients may respond to B cell depleting treatment methods. Our aim was to investigate whether inhibition of Fc receptor–like B (FCRLB) could effectively suppress autoimmunity without diminishing B…
Inhibition of tumor intrinsic BANF1 activates antitumor immune responses via cGAS-STING and enhances the efficacy of PD-1 blockade
Introduction Immune checkpoint inhibitors (ICIs) have revolutionized the treatment pattern of various cancers, including melanoma and non-small cell lung cancer (NSCLC).1–3 However, there are still a considerable proportion (40%–60%) of patients who do not respond or do not achieve durable responses to these drugs.4 Thus, it is urgent clinical need…
Non-cell-autonomous cancer progression from chromosomal instability
Cell culture IMR90, 4T1, CT26, RAW264.7 and B16F10 cell lines were purchased from the American Type Culture Collection and cultured in MEM (IMR90), DMEM (B16F10, RAW264.7) or RPMI (4T1, IMR90, CT26) supplemented with 10% FBS in the presence of penicillin (50 U ml−1) and streptomycin (50 μg ml−1). All cells were found to be…
Anti-Mouse EpCAM (CD326) In Vivo Antibody
Epithelial cell adhesion molecule (EpCAM; also known as CD326 or Tacstd1) is a 40 kDa type I transmembrane glycoprotein composed of an extracellular domain, single transmembrane domain, and the intracellular domain Ep1CD1. EpCAM functions in cell adhesion, signaling, differentiation, migration, proliferation, formation and maintenance of organ morphology, and morphogenic movements…
Establishment and Characterization of Patient-Derived Xenograft Models of Anaplastic Thyroid Carcinoma and Head and Neck Squamous Cell Carcinoma
The present protocol establishes and characterizes a patient-derived xenograft (PDX) model of anaplastic thyroid carcinoma (ATC) and head and neck squamous cell carcinoma (HNSCC), as PDX models are rapidly becoming the standard in the field of translational oncology. This protocol helps establish ATC and HNSCC PDX models which better reflect…
Comparative analysis of carbapenem-resistant K. pneumoniae
Introduction The worldwide spread of Klebsiella pneumoniae is a major global public health threat.1,2 K. pneumoniae is an opportunistic pathogen that colonizes the human gut and respiratory tract and possibly causes infection in immunocompromised hosts. Gut colonized K. pneumoniae is one of the important pathogens causing nosocomial infections, although the…
Applications of CRISPR/Cas9 in the Synthesis of Bioactive Compounds from Medicinal Plants
Allgaier C, Franz S (2015) Risk assessment on the use of herbal medicinal products containing pyrrolizidine alkaloids. Regul Toxicol Pharmacol 73:494–500 CrossRef CAS PubMed Google Scholar Barrangou R (2015) Diversity of CRISPR-Cas immune systems and molecular machines. Genome Biol 16:247 CrossRef PubMed PubMed Central Google Scholar Barrangou R, Fremaux C,…
Taraxasterol suppresses the proliferation and tumor growth of androgen-independent prostate cancer cells through the FGFR2-PI3K/AKT signaling pathway
All experimental protocols were approved by Dali University. All methods were performed in accordance with relevant guidelines and regulations. Reagents and chemicals MEM medium, RPMI-1640 medium, fetal bovine serum (FBS), phosphate-buffered saline (PBS), and penicillin–streptomycin (Pen Strep) were purchased from Biological Industries (Israel). CCK-8 reagent was purchased from Proteintech (Wuhan,…
Molecular reshaping of phage-displayed Interleukin-2 at beta chain receptor interface to obtain potent super-agonists with improved developability profiles
Soft-randomization of phage-displayed hIL-2 beta chain interface revealed hotspots modulating the interaction Soft-randomization was chosen for an initial mutational scanning at hIL-2/hIL-2R beta interface, focused on solvent-exposed (>20%) hIL-2 residues close to the beta chain (<5 Å). A library of 7 × 107 members was obtained by Kunkel mutagenesis15 with spiked mutagenic oligonucleotides…
Bovine colostrum ultrafiltrate supplemented with adult bovine serum and transferrin: An effective fbs substitute for cultivation of vero and CHO-K1 cells
Barnes, D. Serum-free animal cell culture. Biotechniques 5:534–542; 1987. Article CAS Google Scholar Barnes, D.; Sato, G. Methods for growth of cultured cells in serum-free medium. Anal. Biochem. 102:255–270; 1980. Article PubMed CAS Google Scholar Brown, K. D.; Blakeley, D. M. Cell-promoting activity in mammary secretions of the goat, cow…
Chemically programmed STING-activating nano-liposomal vesicles improve anticancer immunity
Rational design of STING-activating pro-drugs for liposomal formulation A recently discovered non-nucleotide STING agonist, MSA-2, was selected to test the validity of our drug design. To facilitate the liposomal formulation of MSA-2, four synthetic MSA-2 derivatives (compounds 1–4) were initially constructed via ester bonds using MP-typed alkanols of varying lengths…
CRISPR/Cas12a combined with RPA for detection of T. gondii in mouse whole blood | Parasites & Vectors
Mice and parasites The strains RH, Pru, wh3, and wh6, utilized in the present investigation, were generously provided by the Anhui Key Laboratory of Zoonoses, Anhui Medical University. The DNA of C. parvum and Babesia were procured from Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences. Furthermore, Plasmodium was…
Evaluation of the immunoprotective effects of eight recombinant proteins from Baylisascaris schroederi in mice model | Parasites & Vectors
Geng GB. 1864 Wild pandas protection in China makes new achievements–the results of the fourth national panda survey. Green Chin. 2015;422:10–12. Zhang JS, Daszak P, Huang HL, Yang GY, Kilpatrick AM, Zhang S. Parasite threat to panda conservation. Ecohealth. 2008;5:6–9. Article PubMed Google Scholar Qin Z, Liu S, Bai M,…
RARRES2 regulates lipid metabolic reprogramming to mediate the development of brain metastasis in triple negative breast cancer | Military Medical Research
Tumor samples from patients Biopsies of primary breast tumors and breast tumors that had metastasized to the brain were obtained from patients with TNBC at the National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital of the Chinese Academy of Medical Sciences and Peking Union Medical College. Single-cell RNA sequencing…
Pathogenic potentials and DNA sequencing of airborne Aspergillus species from Indoor environments in Nigeria
Background: Air pollution by fungal spores poses a potential threat to the health and wellbeing of people. Airborne Aspergillus species play an important role in causing allergenic and invasive infections in susceptible individuals. Objectives: We conducted this study to determine the prevalence and pathogenic potentials of airborne Aspergillus species in…
Crosstalk between KIF1C and PRKAR1A in left atrial myxoma
Two rare variations of KIF1C were identified by WES and RNA-seq Eighteen PBMCs samples and sixteen myxoma tissue samples were included in the research. A flowchart showing the experimental design, sample details, and distribution of variations is shown in Fig. 1a. Sanger sequencing for the exons of PRKAR1A was carried out…
ResR/McdR-regulated protein translation machinery contributes to drug resilience in Mycobacterium tuberculosis
Bacterial strains and culture conditions Escherichia coli strain DH5α (Thermo Fisher) was used for the propagation of plasmids, whereas E. coli BL21 DE3 (Novagen) was used for the expression and purification of ResR/McdR protein. Mtb Erdman was obtained from Dr. Ramandeep Singh at THSTI, India, and Mtb H37Rv mc2 790242…
5’/3′ RACE method for sequencing the 5′ and 3′ untranslated regions of Zika virus
Akiyama BM, Laurence HM, Massey AR, Costantino DA, Xie X, Yang Y, Shi PY, Nix JC, Beckham JD, Kieft JS (2016) Zika virus produces noncoding RNAs using a multi-pseudoknot structure that confounds a cellular exonuclease. Science 354:1148–1152 Article CAS PubMed PubMed Central Google Scholar Alvarez DE, Lodeiro MF, Ludueña SJ,…
Fact Check: New Study Does NOT Prove Pfizer mRNA COVID-19 Vaccine Induces ‘Turbo Cancer’
Not Linked Does a new study prove that the Pfizer mRNA COVID-19 vaccine causes “turbo cancer”? No, that’s not true: The study cited in the social media post doesn’t show that the Pfizer mRNA COVID-19 vaccine induces something called “turbo cancer.” Additionally, a cancer expert wrote, “‘Turbo cancer’ is not…
SARDH shRNA Plasmid Mouse) – Gentaur.com
Catalog number MBS8280461 Price Please ask Size 0,15 mg Other size please contact us to order other different size Test Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40…
Testicular Toxicity in Rats Exposed to AlCl3: a Proteomics Study
Chen H, Chow CL, Lau D (2022) Deterioration mechanisms and advanced inspection technologies of aluminum windows. Materials 15:354. doi.org/10.3390/ma15010354 Article CAS PubMed PubMed Central Google Scholar Chauhan DK, Yadav V, Vaculík M et al (2021) Aluminum toxicity and aluminum stress-induced physiological tolerance responses in higher plants. Crit Rev Biotechnol 41:715–730….
How do tumor exosomes communicate cancer progression?
Exosomes are extracellular vesicles ranging between 30 and 150 nm in diameter that are released from different kinds of cells, including tumor cells. They have the potential to induce apoptosis, modulate the immune system, and work as biomarkers for diagnosis. As a key component of cell-to-cell communication, exosomes can control the…
Non disseminative nano-strategy against in vivo Staphylococcus aureus biofilms
Materials The lipid cetyl palmitate was a kind gift from Gattefossé (Gattefossé SAS, Saint-Priest, France). DSPE-PEG2000-NH2 (1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000] (ammonium salt)) was purchased from Avanti Polar Lipids Inc. (Alabaster, Alabama, USA). D-Phenylalanine, D-Proline, D-Tyrosine, triethylamine (TEA), dicyclohexylcarbodiimide (DCC), N-hydroxysuccinimide (NHS), sodium hydroxide (NaOH), sodium chloride, moxifloxacin hydrochloride (MOX), farnesol 95%, Tween®80,…
How long until effective Helicobacter pylori vaccine – human
Introduction Helicobacter pylori became a concern in 1979 when John Warren, a pathologist in Perth, Western Australia, discovered that the inflamed gastric mucosa was covered with bacteria that looked like Campylobacter.1 Warren and his colleague Barry Marshall cultured the first batch of these bacteria, newly named Helicobacter pylori, subsequently from…
Group B Streptococcus Cas9 variants provide insight into programmable gene repression and CRISPR-Cas transcriptional effects
Amino acid sequence comparisons between GAS and GBS Cas9 identify orthologous active sites Relationships between the amino acid sequence of GBS Cas9 endonuclease and its molecular functions can be deduced from detailed studies of its GAS ortholog, SpyCas9 (Supplementary Fig. 1). As a first step in locating DNA complementary to a…
Robust IL-2-dependent antitumor immunotherapy requires targeting the high-affinity IL-2R on tumor-specific CD8+ T cells
Introduction Interleukin (IL)-2 was the first Food and Drug Administration approved immunotherapy for use in patients with metastatic melanoma and renal cell carcinoma.1 2 However, the use of recombinant IL-2 (Proleukin/aldesleukin) has been problematic due to low response rates, off-target effects, and severe dose-dependent toxicities. Due to its potent T-cell…
FBXW7 promotes autophagy and inhibits proliferation of oral squamous cell carcinoma
Background: F-box and WD repeat domain containing 7 (FBXW7) is a critical tumor suppressor. The expression of FBXW7 is decreased in oral squamous cell carcinoma (OSCC) tissues and shows diagnosis value. We aimed to investigate the influence of FBXW7 overexpression on OSCC cell proliferation and autophagy. Methods: In Balb/c nude…
VCL shRNA Plasmid Mouse) – Gentaur.com
Catalog number MBS8273409 Price Please ask Size 0,15 mg Other size please contact us to order other different size Test Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40…
Human T-cell lymphotropic virus type 1 (HTLV-1) proposed vaccines: a systematic review of preclinical and clinical studies | BMC Infectious Diseases
Characteristics of included studies Based on our search, we retrieved 1700 citations. After removing duplicates, a total of 1250 articles were screened based on title and abstract. Furthermore, the hand-searching of other studies revealed 7 studies that met the inclusion criteria and were included after assessing their full texts. Overall,…
Irreversible electroporation for post-operative margin therapy to prevent cancer recurrence based on triboelectric nanogenerator driven balloon catheter,Nano Energy
Even though cancer therapeutic methods are improving rapidly, cancer recurrence has always remained a big challenge. Even a small lesion of missed neoplastic cells during the surgery can result in tumor recurrence and the consequent silent metastasis, making this issue more critical and complicated for prognosis. Hence, developing new methods…
Irreversible electroporation for post-operative margin therapy to prevent cancer recurrence based on triboelectric nanogenerator driven balloon catheter
Breast cancer is one of the leading cancers in women worldwide [1], [2]. The current standard of care employed for women with early-stage breast cancer is breast conserving surgery (BCT) [3], [4]. BCT offers breast-conserving surgery, including tumor excision (lumpectomy) with an adequate amount of normal neighbor tissue followed by…
A simple paper test could offer early cancer diagnosis
Multiplexed DNA-encoded synthetic urine biomarkers for portable monitoring of invasive colorectal cancer. a, Multiplexed DNA-SUBs are comprised of a polymeric nanocarrier (eight-arm PEG) functionalized with protease-activated peptides barcoded with oligonucleotides. b, Left: Timeline of longitudinal tumor monitoring with DNA-SUB. D, day. Right: histological lung staining of BALB/c mice bearing CRC…
Spontaneous tumor regression mediated by human T cells in a humanized immune system mouse model
Human CD34+ cell isolation Human fetal liver (FL) samples were obtained from Advanced Biosciences Resources (Alameda, CA) with proper consent. FL samples were cut in small fragments, treated for 25 min at 37 °C with Collagenase D (100 ng/mL; Roche). The cell suspension was prepared, and the human CD34+ cells were separated by…
Antigenicity and immunogenicity of SARS-CoV-2 surface glycoprotein fragment in CHO cells
Background and objectives: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) glycoprotein that projects from the virus surface is highly immunogenic. It is considered to be the target of many neutralizing antibodies as well as a target in vaccine design efforts. Evaluation the immunogenicity of a recombinant fragment of…
PRR15 deficiency facilitates malignant progression by mediating PI3K/Akt signaling and predicts clinical prognosis in triple-negative rather than non-triple-negative breast cancer
Cell culture and reagents Non-cancerous mammary epithelium cell (MCF10A), breast cancer cells including luminal (MCF7, MDA-MB-361, T47D, and BT474), HER2amp (SKBR3), and triple-negative (MDA-MB-231, CAL51, BT20, and MDA-MB-468) subtypes, as well as human embryonic kidney 293T (HEK-293T) cells, were purchased from the American Type Culture Collection (Manassas, VA, USA). MDA-MB-231,…
Genetic susceptibility to diabetic kidney disease is linked to promoter variants of XOR
Collins, A. J. et al. Excerpts from the United States Renal Data System 2004 annual data report: atlas of end-stage renal disease in the United States. Am. J. Kidney Dis. 45, A5–A7, S1–280 (2005). Article Google Scholar Ogurtsova, K. et al. IDF Diabetes Atlas: global estimates for the prevalence of…
Ras interacting protein 1 facilitated proliferation and invasion of diffuse large B-cell lymphoma cells
Introduction Diffuse large B-cell lymphoma (DLBCL) is the most common lymphoma, accounting for 30% of non-Hodgkin’s lymphoma. Approximately 150,000 new cases are diagnosed annually worldwideCitation1. DLBCL is characterized by heterogeneity, aggressiveness, and frequent relapse or resistance to chemotherapyCitation2. Due to the heterogeneity of DLBCL, the immunological, pathological, molecular, and genetic…
Toxins | Free Full-Text | Metagenome Analysis Identifies Microbial Shifts upon Deoxynivalenol Exposure and Post-Exposure Recovery in the Mouse Gut
1. Introduction Fusarium fungi are world-wide producers of a range of mycotoxins. Deoxynivalenol (DON) belonging to the group B trichothecenes, is one of the most prevalent food-associated mycotoxins mainly produced by Fusarium graminearum and Fusarium culmorum, and frequently contaminates cereals and cereal products [1,2]. Almost half of a total of…
Intranasal trimeric sherpabody inhibits SARS-CoV-2 including recent immunoevasive Omicron subvariants
Research reported in this study complies with all relevant ethical regulations, and our animal study protocol has been approved by the Animal Experimental Board of Finland (license number ESAVI/28687/2020. Phage panning Sherpabody phage display library (size ~1011 cfu) was obtained from Next Biomed Therapies Oy. To develop sherpabodies specific for SARS-CoV-2…
DNA origami presenting the receptor binding domain of SARS-CoV-2 elicit robust protective immune response
Reagents, consumables, and kits The oligonucleotides used as primers in the asymmetric polymerase chain reaction (aPCR) and as staple strands for the folding of PB NPs, along with the CpG ODN 1018 adjuvant were purchased from Integrated DNA Technologies (IDT). Fluorescently labelled oligonucleotides used in FRET assay, FAM (donor) and…
Development and validation of a machine learning algorithm prediction for dense granule proteins in Apicomplexa | Parasites & Vectors
Collection of the dataset High quality of data is crucial for prediction models. To ensure the universality of the model, we first construct a GRA training set consisting of 245 positive samples and 1706 negative samples. The DGPD includes GRA information on some important apicomplexan parasites [22]. We retrieved 245…
Reduction of the accumulation of viral mRNA and of cellular rRNA synthesis in MHV3-infected macrophages of resistant mice | Braz. j. med. biol. res;27(3): 601-11, Mar. 1994. ilus, graf
RESUMO 1. After MHV3 infection, only macrophages from resistant A/J mice partially restricted virus growth compared to those from susceptible BALB/c mice (2 logs of difference in virus titer). 2. Cellular ribosomal ribonucleic acid (rRNA) synthesis by MHV3-infected macrophages was decreased only in A/J mouse macrophages as indicated by accumulation…
The DNA damage response pathway regulates the expression of the immune checkpoint CD47
Chan, K. S. et al. Identification, molecular characterization, clinical prognosis, and therapeutic targeting of human bladder tumor-initiating cells. Proc. Natl Acad. Sci. USA 106, 14016–14021 (2009). Article CAS PubMed PubMed Central Google Scholar Adams, S. et al. Signal-regulatory protein is selectively expressed by myeloid and neuronal cells. J. Immunol. 161,…
CircDHRS3 inhibits prostate cancer cell proliferation and metastasis through the circDHRS3/miR-421/MEIS2 axis
. 2023 Dec;18(1):2178802. doi: 10.1080/15592294.2023.2178802. Affiliations Expand Affiliations 1 Department of Urology, Huashan Hospital, Fudan University, Shanghai, China. 2 Fudan Institute of Urology, Huashan Hospital, Fudan University, Shanghai, China. 3 National Clinical Research Center for Aging and Medicine, Fudan University, Shanghai, China. Item in Clipboard Xiyu Dai et al. Epigenetics. 2023…
RAGA prevents tumor immune evasion of LUAD by promoting CD47 lysosome degradation
RAGA regulates lung adenocarcinoma progression and macrophage phagocytosis We performed experiments to explore the role of RAGA in lung adenocarcinoma by in vitro cell culture and in vivo xenograft system. To this end, we generated RAGA knockdown A549 and H1299 cancer cells. In vitro cell proliferation assays showed that RAGA…
Custom genome installation in SigProfileMatrixGenerator
Custom genome installation in SigProfileMatrixGenerator 1 I would be interested to hear from anyone who has added a custom genome (other than those created by the authors) to SigProfilerMatrixGenerator. github.com/AlexandrovLab/SigProfilerMatrixGenerator I have been able to successfully run it with ‘mm39’, but have been unable to install a genome for BALB_cJ….
Figures and data in Directed differentiation of human iPSCs to functional ovarian granulosa-like cells via transcription factor overexpression
Strain, strain background (Mus musculus, female) CD-1 Charles River Labs RRID:IMSR_CRL:022 Used for fetal ovary isolation Strain, strain background (Mus musculus, female) BALB/c Charles River Labs RRID:IMSR_APB:4790 Used for adult ovary isolation Cell line (Homo sapiens, female) F3 iPSC ATCC ATCC-BXS0116 Cell line (Homo sapiens, female) F66 iPSC Other Previously…
Polydopamine-Liposome as Versatile Theranostics Nanoplatform
Introduction According to the global cancer statistics 2018, breast cancer has the highest global incidence (28.9%) and mortality rates (23.0%) in women.1 Chemotherapy, as a mainstay in clinical breast cancer treatment, is limited by severe side-effects resulting from the nonspecific drug distribution in the human body.2–4 What is worse, a…
Damage to Nuclear and Mitochondrial DNA in Different Organs in Streptozotocin-Induced Diabetes Models in BALB/c Mice
Male BALB/c mice with streptozotocin-induced diabetes mellitus were used to study nuclear DNA (nDNA) and mitochondrial DNA (mtDNA) damage using comet DNA assay and real-time PCR, respectively. In animals receiving single injection of streptozotocin in a dose of 200 mg/kg, severe hyperglycemia was observed on days 10 and 21 of…
CircRNA Circ_0000118 Regulates Malignancy of Cervical Cancer Cells by Regulating miR-211-5p/miR-377-3p/AKT2 Axis
Samples and Cell Lines A total of 78 pairs of tumor samples and the adjacent non-carcinoma control samples were collected by surgery from CC patients in our study. The peripheral blood was also sampled from each individual and the serum was prepared and preserved under − 80 °C till further analysis. The Medical…
circRNA ITGA7 restrains growth and enhances radiosensitivity by up-regulating SMAD4 in colorectal carcinoma
Abstract Circular RNAs have been reported to be widely involved in cancer cell tumorigenesis and drug resistance; here, the aim of this study was to investigate whether circRNA Integrin Subunit Alpha 7 (ITGA7) (circ_ITGA7) was associated with the tumor growth and radiosensitivity of colorectal cancer (CRC). We found that circ_ITGA7…
Samd11 MGI Mouse Gene Detail – MGI:2446220
Strain Gene Model ID Feature Type Coordinates Select Strains C57BL/6J MGI_C57BL6J_2446220 protein coding gene Chr4:156331423-156342409 (-) 129S1/SvImJ MGP_129S1SvImJ_G0029254 protein coding gene Chr4:160619461-160628390 (-) A/J MGP_AJ_G0029219 protein coding gene Chr4:153853593-153863429 (-) AKR/J MGP_AKRJ_G0029167 protein coding gene Chr4:158046776-158056762 (-) BALB/cJ MGP_BALBcJ_G0029235 protein coding gene Chr4:154329846-154338966 (-) C3H/HeJ MGP_C3HHeJ_G0028956 protein coding gene Chr4:158622601-158632726…
Effects of different applied voltages of irreversible electroporation on prostate cancer in a mouse model
Cell culture The human prostate cancer cell line (DU145) was purchased from Korean Cell Line Bank (Pohang, Korea). The cells were cultured in Roswell Park Memorial Institute 1640 medium with L-glutamine (300 mg/L), 25 mM HEPES, and 25 mM NaHCO3 and supplemented with 10% fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MI, USA),…
IMUNON, INC. MANAGEMENT’S DISCUSSION AND ANALYSIS OF FINANCIAL CONDITION AND RESULTS OF OPERATIONS. (form 10-Q)
The following discussion and analysis of our financial condition and results of operations This discussion contains forward-looking statements that involve risks and uncertainties. Our actual results may differ materially from those discussed in forward-looking statements. Factors that might cause a difference include, but are not limited to, those discussed above…
A potent, broadly protective vaccine against SARS-CoV-2 variants of concern
Cells and viruses hACE2 expressing BHK-21 (BHK-21-hACE2) and Vero-E6 cells were provided by State Key Laboratory of Virology, Wuhan University. Vero-E6 cells were cultured in DMEM (Gibco, Cat.11995–040) supplemented with 10% fetal bovine serum (Gibco, Cat.10091–148) and 1% penicillin and streptomycin solution (BasalMedia, Cat.S110JV) at 37°C with 5% CO2. BHK-21-hACE2…
Breadth of SARS-CoV-2 neutralization and protection induced by a nanoparticle vaccine
Animals and immunizations The study protocol and all veterinarian procedures were approved by the Bioqual IACUC per a memorandum of understanding with the Duke IACUC, and were performed based on standard operating procedures. Macaques studied were housed and maintained in an Association for Assessment and Accreditation of Laboratory Animal Care-accredited…
Proscillaridin A induces mitochondrial damage and autophagy in pancreatic cancer and reduces the stability of SMAD4 in Panc-1 cells – Hou
Introduction Pancreatic cancer (PC) is one of the most aggressive and lethal cancers (1). Previous studies have shown that aging, environmental and behavioral changes are more closely related to the dramatic increase in the incidence of PC worldwide than genetic factors (2–5). A position paper published by the European Federation…
Circular RNA circ-BNC2 (hsa_circ_0008732) inhibits the progression of ovarian cancer through microRNA-223-3p/ FBXW7 axis | Journal of Ovarian Research
Ethics statement and patient samples 40 pairs of OC tissues and adjacent tissue samples were collected from patients who were diagnosed with OC and received surgical resection in the Affiliated Hospital of Zunyi Medical University. None of the patients received any anti-cancer treatment before surgery, and all samples were immediately…
Two DNA vaccines protect against severe disease and pathology due to SARS-CoV-2 in Syrian hamsters
This article was originally published here NPJ Vaccines. 2022 Apr 26;7(1):49. doi: 10.1038/s41541-022-00461-5. ABSTRACT The SARS-CoV-2 pandemic is an ongoing threat to global health, and wide-scale vaccination is an efficient method to reduce morbidity and mortality. We designed and evaluated two DNA plasmid vaccines, based on the pIDV-II system, expressing…
N6-methyladenosine modification of CENPK mRNA by ZC3H13 promotes cervical cancer stemness and chemoresistance | Military Medical Research
Bioinformatics analyses revealed the involvement of m6A modification in cervical cancer progression To better understand whether and how m6A regulators contribute to cervical cancer progression, we first identified 9 m6A writers (WTAP, ZC3H13, METTL3, METTL14, METTL16, VIRMA, RBM15B, RBM15, and CBLL1), 15 m6A readers (FMR1, hnRNPA2B1, hnRNPC, YTHDF1/2/3, YTHDC1/2, LRPPRC,…
Development of PNC-27 targeted codelivery system for survivin-shRNA and SN38 against colon adenocarcinoma in vitro and in vivo
doi.org/10.1016/j.jddst.2022.103180Get rights and content Highlights • SN38 was encapsulated in PEI-conjugated SWCNT based on π-π stacking interaction. • Survivin shRNA was further condensed in the prepared carrier. • Codelivery of SN38 and shRNA increased therapeutic efficacy and synergistic effect. • Tagging of the codelivery system with PNC27 significantly enhanced therapeutic…
MouseMine: Keyword Search
Search our database by keyword Examples Search this entire website. Enter identifiers, names or keywords for genes, diseases, strains, ontology terms, etc. (e.g. Pax6, Parkinson, ataxia) Use OR to search for either of two terms (e.g. OR mus) or quotation marks to search for phrases (e.g. “dna binding”). Boolean search…
Establishment of sunitinib-resistant CDX model of ccRCC
Introduction Renal cell carcinoma (RCC) accounts for approximately 2–3% of all malignant tumors, and its prevalence is rising. Metastatic RCC accounts for 25–30% of all RCC cases, and has an exceedingly poor prognosis.1 In 2020, among approximately 430,000 newly discovered cases of RCC, 179,000 died.2 Clear cell renal cell carcinoma…
Single-cell DNA and RNA sequencing reveals the dynamics of intra-tumor heterogeneity in a colorectal cancer model | BMC Biology
Organoid culture of small intestinal cells and lentiviral transduction C57BL/6J mice and BALB/cAnu/nu immune-deficient nude mice were purchased from CLEA Japan (Tokyo, Japan). The small intestine was harvested from wild-type male C57BL/6J mice at 3–5 weeks of age (Additional file 1: Figure S9A). Crypts were purified and dissociated into single cells,…
Modification of endoglin-targeting nanoliposomes | IJN
Introduction Today, malignant tumors (cancer) still severely imperil human health and cause millions of global mortality rates.1,2 Deep-seated solid tumors are challenging to cure by most therapeutic tools, mainly blamed on the complex tumor microenvironment (TME).3,4 Adoptive cell therapy (ACT), as one of the effective immunotherapeutic means for cancer treatment,…
Genome-wide synthetic lethal screen unveils novel CAIX-NFS1/xCT axis as a targetable vulnerability in hypoxic solid tumors
Abstract The metabolic mechanisms involved in the survival of tumor cells within the hypoxic niche remain unclear. We carried out a synthetic lethal CRISPR screen to identify survival mechanisms governed by the tumor hypoxia–induced pH regulator carbonic anhydrase IX (CAIX). We identified a redox homeostasis network containing the iron-sulfur cluster…
Pericentromeric noncoding RNA changes DNA binding of CTCF and inflammatory gene expression in senescence and cancer
Significance During the aging process, senescent cells secrete inflammatory factors, causing various age-related pathologies. Thus, controlling the senescence-associated secretory phenotype (SASP) can tremendously benefit human health. Although SASP seems to be induced by the alteration of chromosomal organization, its underlying mechanism remains unclear. Here, it has been revealed that noncoding…
Humanized Mice: Generation, Advantages and Applications
Mice are commonly used as models of human disease, helping scientists to understand the workings of complex pathologies and develop safe and efficacious drugs. It is now possible to create a range of genetically engineered mouse models, including humanized, knock-in and knock-out models, that can be used to further advance…