Tag: BALB

Robust IL-2-dependent antitumor immunotherapy requires targeting the high-affinity IL-2R on tumor-specific CD8+ T cells

Introduction Interleukin (IL)-2 was the first Food and Drug Administration approved immunotherapy for use in patients with metastatic melanoma and renal cell carcinoma.1 2 However, the use of recombinant IL-2 (Proleukin/aldesleukin) has been problematic due to low response rates, off-target effects, and severe dose-dependent toxicities. Due to its potent T-cell…

Continue Reading Robust IL-2-dependent antitumor immunotherapy requires targeting the high-affinity IL-2R on tumor-specific CD8+ T cells

FBXW7 promotes autophagy and inhibits proliferation of oral squamous cell carcinoma

Background: F-box and WD repeat domain containing 7 (FBXW7) is a critical tumor suppressor. The expression of FBXW7 is decreased in oral squamous cell carcinoma (OSCC) tissues and shows diagnosis value. We aimed to investigate the influence of FBXW7 overexpression on OSCC cell proliferation and autophagy. Methods: In Balb/c nude…

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VCL shRNA Plasmid Mouse) – Gentaur.com

Catalog number MBS8273409 Price Please ask Size 0,15 mg Other size please contact us to order other different size Test Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40…

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Human T-cell lymphotropic virus type 1 (HTLV-1) proposed vaccines: a systematic review of preclinical and clinical studies | BMC Infectious Diseases

Characteristics of included studies Based on our search, we retrieved 1700 citations. After removing duplicates, a total of 1250 articles were screened based on title and abstract. Furthermore, the hand-searching of other studies revealed 7 studies that met the inclusion criteria and were included after assessing their full texts. Overall,…

Continue Reading Human T-cell lymphotropic virus type 1 (HTLV-1) proposed vaccines: a systematic review of preclinical and clinical studies | BMC Infectious Diseases

Irreversible electroporation for post-operative margin therapy to prevent cancer recurrence based on triboelectric nanogenerator driven balloon catheter,Nano Energy

Even though cancer therapeutic methods are improving rapidly, cancer recurrence has always remained a big challenge. Even a small lesion of missed neoplastic cells during the surgery can result in tumor recurrence and the consequent silent metastasis, making this issue more critical and complicated for prognosis. Hence, developing new methods…

Continue Reading Irreversible electroporation for post-operative margin therapy to prevent cancer recurrence based on triboelectric nanogenerator driven balloon catheter,Nano Energy

Irreversible electroporation for post-operative margin therapy to prevent cancer recurrence based on triboelectric nanogenerator driven balloon catheter

Breast cancer is one of the leading cancers in women worldwide [1], [2]. The current standard of care employed for women with early-stage breast cancer is breast conserving surgery (BCT) [3], [4]. BCT offers breast-conserving surgery, including tumor excision (lumpectomy) with an adequate amount of normal neighbor tissue followed by…

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A simple paper test could offer early cancer diagnosis

Multiplexed DNA-encoded synthetic urine biomarkers for portable monitoring of invasive colorectal cancer. a, Multiplexed DNA-SUBs are comprised of a polymeric nanocarrier (eight-arm PEG) functionalized with protease-activated peptides barcoded with oligonucleotides. b, Left: Timeline of longitudinal tumor monitoring with DNA-SUB. D, day. Right: histological lung staining of BALB/c mice bearing CRC…

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Spontaneous tumor regression mediated by human T cells in a humanized immune system mouse model

Human CD34+ cell isolation Human fetal liver (FL) samples were obtained from Advanced Biosciences Resources (Alameda, CA) with proper consent. FL samples were cut in small fragments, treated for 25 min at 37 °C with Collagenase D (100 ng/mL; Roche). The cell suspension was prepared, and the human CD34+ cells were separated by…

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Antigenicity and immunogenicity of SARS-CoV-2 surface glycoprotein fragment in CHO cells

Background and objectives: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) glycoprotein that projects from the virus surface is highly immunogenic. It is considered to be the target of many neutralizing antibodies as well as a target in vaccine design efforts. Evaluation the immunogenicity of a recombinant fragment of…

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PRR15 deficiency facilitates malignant progression by mediating PI3K/Akt signaling and predicts clinical prognosis in triple-negative rather than non-triple-negative breast cancer

Cell culture and reagents Non-cancerous mammary epithelium cell (MCF10A), breast cancer cells including luminal (MCF7, MDA-MB-361, T47D, and BT474), HER2amp (SKBR3), and triple-negative (MDA-MB-231, CAL51, BT20, and MDA-MB-468) subtypes, as well as human embryonic kidney 293T (HEK-293T) cells, were purchased from the American Type Culture Collection (Manassas, VA, USA). MDA-MB-231,…

Continue Reading PRR15 deficiency facilitates malignant progression by mediating PI3K/Akt signaling and predicts clinical prognosis in triple-negative rather than non-triple-negative breast cancer

Genetic susceptibility to diabetic kidney disease is linked to promoter variants of XOR

Collins, A. J. et al. Excerpts from the United States Renal Data System 2004 annual data report: atlas of end-stage renal disease in the United States. Am. J. Kidney Dis. 45, A5–A7, S1–280 (2005). Article  Google Scholar  Ogurtsova, K. et al. IDF Diabetes Atlas: global estimates for the prevalence of…

Continue Reading Genetic susceptibility to diabetic kidney disease is linked to promoter variants of XOR

Ras interacting protein 1 facilitated proliferation and invasion of diffuse large B-cell lymphoma cells

Introduction Diffuse large B-cell lymphoma (DLBCL) is the most common lymphoma, accounting for 30% of non-Hodgkin’s lymphoma. Approximately 150,000 new cases are diagnosed annually worldwideCitation1. DLBCL is characterized by heterogeneity, aggressiveness, and frequent relapse or resistance to chemotherapyCitation2. Due to the heterogeneity of DLBCL, the immunological, pathological, molecular, and genetic…

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Toxins | Free Full-Text | Metagenome Analysis Identifies Microbial Shifts upon Deoxynivalenol Exposure and Post-Exposure Recovery in the Mouse Gut

1. Introduction Fusarium fungi are world-wide producers of a range of mycotoxins. Deoxynivalenol (DON) belonging to the group B trichothecenes, is one of the most prevalent food-associated mycotoxins mainly produced by Fusarium graminearum and Fusarium culmorum, and frequently contaminates cereals and cereal products [1,2]. Almost half of a total of…

Continue Reading Toxins | Free Full-Text | Metagenome Analysis Identifies Microbial Shifts upon Deoxynivalenol Exposure and Post-Exposure Recovery in the Mouse Gut

Intranasal trimeric sherpabody inhibits SARS-CoV-2 including recent immunoevasive Omicron subvariants

Research reported in this study complies with all relevant ethical regulations, and our animal study protocol has been approved by the Animal Experimental Board of Finland (license number ESAVI/28687/2020. Phage panning Sherpabody phage display library (size ~1011 cfu) was obtained from Next Biomed Therapies Oy. To develop sherpabodies specific for SARS-CoV-2…

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DNA origami presenting the receptor binding domain of SARS-CoV-2 elicit robust protective immune response

Reagents, consumables, and kits The oligonucleotides used as primers in the asymmetric polymerase chain reaction (aPCR) and as staple strands for the folding of PB NPs, along with the CpG ODN 1018 adjuvant were purchased from Integrated DNA Technologies (IDT). Fluorescently labelled oligonucleotides used in FRET assay, FAM (donor) and…

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Development and validation of a machine learning algorithm prediction for dense granule proteins in Apicomplexa | Parasites & Vectors

Collection of the dataset High quality of data is crucial for prediction models. To ensure the universality of the model, we first construct a GRA training set consisting of 245 positive samples and 1706 negative samples. The DGPD includes GRA information on some important apicomplexan parasites [22]. We retrieved 245…

Continue Reading Development and validation of a machine learning algorithm prediction for dense granule proteins in Apicomplexa | Parasites & Vectors

Reduction of the accumulation of viral mRNA and of cellular rRNA synthesis in MHV3-infected macrophages of resistant mice | Braz. j. med. biol. res;27(3): 601-11, Mar. 1994. ilus, graf

RESUMO 1. After MHV3 infection, only macrophages from resistant A/J mice partially restricted virus growth compared to those from susceptible BALB/c mice (2 logs of difference in virus titer). 2. Cellular ribosomal ribonucleic acid (rRNA) synthesis by MHV3-infected macrophages was decreased only in A/J mouse macrophages as indicated by accumulation…

Continue Reading Reduction of the accumulation of viral mRNA and of cellular rRNA synthesis in MHV3-infected macrophages of resistant mice | Braz. j. med. biol. res;27(3): 601-11, Mar. 1994. ilus, graf

The DNA damage response pathway regulates the expression of the immune checkpoint CD47

Chan, K. S. et al. Identification, molecular characterization, clinical prognosis, and therapeutic targeting of human bladder tumor-initiating cells. Proc. Natl Acad. Sci. USA 106, 14016–14021 (2009). Article  CAS  PubMed  PubMed Central  Google Scholar  Adams, S. et al. Signal-regulatory protein is selectively expressed by myeloid and neuronal cells. J. Immunol. 161,…

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CircDHRS3 inhibits prostate cancer cell proliferation and metastasis through the circDHRS3/miR-421/MEIS2 axis

. 2023 Dec;18(1):2178802. doi: 10.1080/15592294.2023.2178802. Affiliations Expand Affiliations 1 Department of Urology, Huashan Hospital, Fudan University, Shanghai, China. 2 Fudan Institute of Urology, Huashan Hospital, Fudan University, Shanghai, China. 3 National Clinical Research Center for Aging and Medicine, Fudan University, Shanghai, China. Item in Clipboard Xiyu Dai et al. Epigenetics. 2023…

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RAGA prevents tumor immune evasion of LUAD by promoting CD47 lysosome degradation

RAGA regulates lung adenocarcinoma progression and macrophage phagocytosis We performed experiments to explore the role of RAGA in lung adenocarcinoma by in vitro cell culture and in vivo xenograft system. To this end, we generated RAGA knockdown A549 and H1299 cancer cells. In vitro cell proliferation assays showed that RAGA…

Continue Reading RAGA prevents tumor immune evasion of LUAD by promoting CD47 lysosome degradation

Custom genome installation in SigProfileMatrixGenerator

Custom genome installation in SigProfileMatrixGenerator 1 I would be interested to hear from anyone who has added a custom genome (other than those created by the authors) to SigProfilerMatrixGenerator. github.com/AlexandrovLab/SigProfilerMatrixGenerator I have been able to successfully run it with ‘mm39’, but have been unable to install a genome for BALB_cJ….

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Figures and data in Directed differentiation of human iPSCs to functional ovarian granulosa-like cells via transcription factor overexpression

Strain, strain background (Mus musculus, female) CD-1 Charles River Labs RRID:IMSR_CRL:022 Used for fetal ovary isolation Strain, strain background (Mus musculus, female) BALB/c Charles River Labs RRID:IMSR_APB:4790 Used for adult ovary isolation Cell line (Homo sapiens, female) F3 iPSC ATCC ATCC-BXS0116 Cell line (Homo sapiens, female) F66 iPSC Other Previously…

Continue Reading Figures and data in Directed differentiation of human iPSCs to functional ovarian granulosa-like cells via transcription factor overexpression

Polydopamine-Liposome as Versatile Theranostics Nanoplatform

Introduction According to the global cancer statistics 2018, breast cancer has the highest global incidence (28.9%) and mortality rates (23.0%) in women.1 Chemotherapy, as a mainstay in clinical breast cancer treatment, is limited by severe side-effects resulting from the nonspecific drug distribution in the human body.2–4 What is worse, a…

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Damage to Nuclear and Mitochondrial DNA in Different Organs in Streptozotocin-Induced Diabetes Models in BALB/c Mice

Male BALB/c mice with streptozotocin-induced diabetes mellitus were used to study nuclear DNA (nDNA) and mitochondrial DNA (mtDNA) damage using comet DNA assay and real-time PCR, respectively. In animals receiving single injection of streptozotocin in a dose of 200 mg/kg, severe hyperglycemia was observed on days 10 and 21 of…

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CircRNA Circ_0000118 Regulates Malignancy of Cervical Cancer Cells by Regulating miR-211-5p/miR-377-3p/AKT2 Axis

Samples and Cell Lines A total of 78 pairs of tumor samples and the adjacent non-carcinoma control samples were collected by surgery from CC patients in our study. The peripheral blood was also sampled from each individual and the serum was prepared and preserved under − 80 °C till further analysis. The Medical…

Continue Reading CircRNA Circ_0000118 Regulates Malignancy of Cervical Cancer Cells by Regulating miR-211-5p/miR-377-3p/AKT2 Axis

circRNA ITGA7 restrains growth and enhances radiosensitivity by up-regulating SMAD4 in colorectal carcinoma

Abstract Circular RNAs have been reported to be widely involved in cancer cell tumorigenesis and drug resistance; here, the aim of this study was to investigate whether circRNA Integrin Subunit Alpha 7 (ITGA7) (circ_ITGA7) was associated with the tumor growth and radiosensitivity of colorectal cancer (CRC). We found that circ_ITGA7…

Continue Reading circRNA ITGA7 restrains growth and enhances radiosensitivity by up-regulating SMAD4 in colorectal carcinoma

Samd11 MGI Mouse Gene Detail – MGI:2446220

Strain Gene Model ID Feature Type Coordinates Select Strains C57BL/6J MGI_C57BL6J_2446220 protein coding gene Chr4:156331423-156342409 (-) 129S1/SvImJ MGP_129S1SvImJ_G0029254 protein coding gene Chr4:160619461-160628390 (-) A/J MGP_AJ_G0029219 protein coding gene Chr4:153853593-153863429 (-) AKR/J MGP_AKRJ_G0029167 protein coding gene Chr4:158046776-158056762 (-) BALB/cJ MGP_BALBcJ_G0029235 protein coding gene Chr4:154329846-154338966 (-) C3H/HeJ MGP_C3HHeJ_G0028956 protein coding gene Chr4:158622601-158632726…

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Effects of different applied voltages of irreversible electroporation on prostate cancer in a mouse model

Cell culture The human prostate cancer cell line (DU145) was purchased from Korean Cell Line Bank (Pohang, Korea). The cells were cultured in Roswell Park Memorial Institute 1640 medium with L-glutamine (300 mg/L), 25 mM HEPES, and 25 mM NaHCO3 and supplemented with 10% fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MI, USA),…

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IMUNON, INC. MANAGEMENT’S DISCUSSION AND ANALYSIS OF FINANCIAL CONDITION AND RESULTS OF OPERATIONS. (form 10-Q)

The following discussion and analysis of our financial condition and results of operations This discussion contains forward-looking statements that involve risks and uncertainties. Our actual results may differ materially from those discussed in forward-looking statements. Factors that might cause a difference include, but are not limited to, those discussed above…

Continue Reading IMUNON, INC. MANAGEMENT’S DISCUSSION AND ANALYSIS OF FINANCIAL CONDITION AND RESULTS OF OPERATIONS. (form 10-Q)

A potent, broadly protective vaccine against SARS-CoV-2 variants of concern

Cells and viruses hACE2 expressing BHK-21 (BHK-21-hACE2) and Vero-E6 cells were provided by State Key Laboratory of Virology, Wuhan University. Vero-E6 cells were cultured in DMEM (Gibco, Cat.11995–040) supplemented with 10% fetal bovine serum (Gibco, Cat.10091–148) and 1% penicillin and streptomycin solution (BasalMedia, Cat.S110JV) at 37°C with 5% CO2. BHK-21-hACE2…

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Breadth of SARS-CoV-2 neutralization and protection induced by a nanoparticle vaccine

Animals and immunizations The study protocol and all veterinarian procedures were approved by the Bioqual IACUC per a memorandum of understanding with the Duke IACUC, and were performed based on standard operating procedures. Macaques studied were housed and maintained in an Association for Assessment and Accreditation of Laboratory Animal Care-accredited…

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Proscillaridin A induces mitochondrial damage and autophagy in pancreatic cancer and reduces the stability of SMAD4 in Panc-1 cells – Hou

Introduction Pancreatic cancer (PC) is one of the most aggressive and lethal cancers (1). Previous studies have shown that aging, environmental and behavioral changes are more closely related to the dramatic increase in the incidence of PC worldwide than genetic factors (2–5). A position paper published by the European Federation…

Continue Reading Proscillaridin A induces mitochondrial damage and autophagy in pancreatic cancer and reduces the stability of SMAD4 in Panc-1 cells – Hou

Circular RNA circ-BNC2 (hsa_circ_0008732) inhibits the progression of ovarian cancer through microRNA-223-3p/ FBXW7 axis | Journal of Ovarian Research

Ethics statement and patient samples 40 pairs of OC tissues and adjacent tissue samples were collected from patients who were diagnosed with OC and received surgical resection in the Affiliated Hospital of Zunyi Medical University. None of the patients received any anti-cancer treatment before surgery, and all samples were immediately…

Continue Reading Circular RNA circ-BNC2 (hsa_circ_0008732) inhibits the progression of ovarian cancer through microRNA-223-3p/ FBXW7 axis | Journal of Ovarian Research

Two DNA vaccines protect against severe disease and pathology due to SARS-CoV-2 in Syrian hamsters

This article was originally published here NPJ Vaccines. 2022 Apr 26;7(1):49. doi: 10.1038/s41541-022-00461-5. ABSTRACT The SARS-CoV-2 pandemic is an ongoing threat to global health, and wide-scale vaccination is an efficient method to reduce morbidity and mortality. We designed and evaluated two DNA plasmid vaccines, based on the pIDV-II system, expressing…

Continue Reading Two DNA vaccines protect against severe disease and pathology due to SARS-CoV-2 in Syrian hamsters

N6-methyladenosine modification of CENPK mRNA by ZC3H13 promotes cervical cancer stemness and chemoresistance | Military Medical Research

Bioinformatics analyses revealed the involvement of m6A modification in cervical cancer progression To better understand whether and how m6A regulators contribute to cervical cancer progression, we first identified 9 m6A writers (WTAP, ZC3H13, METTL3, METTL14, METTL16, VIRMA, RBM15B, RBM15, and CBLL1), 15 m6A readers (FMR1, hnRNPA2B1, hnRNPC, YTHDF1/2/3, YTHDC1/2, LRPPRC,…

Continue Reading N6-methyladenosine modification of CENPK mRNA by ZC3H13 promotes cervical cancer stemness and chemoresistance | Military Medical Research

Development of PNC-27 targeted codelivery system for survivin-shRNA and SN38 against colon adenocarcinoma in vitro and in vivo

doi.org/10.1016/j.jddst.2022.103180Get rights and content Highlights • SN38 was encapsulated in PEI-conjugated SWCNT based on π-π stacking interaction. • Survivin shRNA was further condensed in the prepared carrier. • Codelivery of SN38 and shRNA increased therapeutic efficacy and synergistic effect. • Tagging of the codelivery system with PNC27 significantly enhanced therapeutic…

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MouseMine: Keyword Search

Search our database by keyword Examples Search this entire website. Enter identifiers, names or keywords for genes, diseases, strains, ontology terms, etc. (e.g. Pax6, Parkinson, ataxia) Use OR to search for either of two terms (e.g. OR mus) or quotation marks to search for phrases (e.g. “dna binding”). Boolean search…

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Establishment of sunitinib-resistant CDX model of ccRCC

Introduction Renal cell carcinoma (RCC) accounts for approximately 2–3% of all malignant tumors, and its prevalence is rising. Metastatic RCC accounts for 25–30% of all RCC cases, and has an exceedingly poor prognosis.1 In 2020, among approximately 430,000 newly discovered cases of RCC, 179,000 died.2 Clear cell renal cell carcinoma…

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Single-cell DNA and RNA sequencing reveals the dynamics of intra-tumor heterogeneity in a colorectal cancer model | BMC Biology

Organoid culture of small intestinal cells and lentiviral transduction C57BL/6J mice and BALB/cAnu/nu immune-deficient nude mice were purchased from CLEA Japan (Tokyo, Japan). The small intestine was harvested from wild-type male C57BL/6J mice at 3–5 weeks of age (Additional file 1: Figure S9A). Crypts were purified and dissociated into single cells,…

Continue Reading Single-cell DNA and RNA sequencing reveals the dynamics of intra-tumor heterogeneity in a colorectal cancer model | BMC Biology

Modification of endoglin-targeting nanoliposomes | IJN

Introduction Today, malignant tumors (cancer) still severely imperil human health and cause millions of global mortality rates.1,2 Deep-seated solid tumors are challenging to cure by most therapeutic tools, mainly blamed on the complex tumor microenvironment (TME).3,4 Adoptive cell therapy (ACT), as one of the effective immunotherapeutic means for cancer treatment,…

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Genome-wide synthetic lethal screen unveils novel CAIX-NFS1/xCT axis as a targetable vulnerability in hypoxic solid tumors

Abstract The metabolic mechanisms involved in the survival of tumor cells within the hypoxic niche remain unclear. We carried out a synthetic lethal CRISPR screen to identify survival mechanisms governed by the tumor hypoxia–induced pH regulator carbonic anhydrase IX (CAIX). We identified a redox homeostasis network containing the iron-sulfur cluster…

Continue Reading Genome-wide synthetic lethal screen unveils novel CAIX-NFS1/xCT axis as a targetable vulnerability in hypoxic solid tumors

Pericentromeric noncoding RNA changes DNA binding of CTCF and inflammatory gene expression in senescence and cancer

Significance During the aging process, senescent cells secrete inflammatory factors, causing various age-related pathologies. Thus, controlling the senescence-associated secretory phenotype (SASP) can tremendously benefit human health. Although SASP seems to be induced by the alteration of chromosomal organization, its underlying mechanism remains unclear. Here, it has been revealed that noncoding…

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Humanized Mice: Generation, Advantages and Applications

Mice are commonly used as models of human disease, helping scientists to understand the workings of complex pathologies and develop safe and efficacious drugs. It is now possible to create a range of genetically engineered mouse models, including humanized, knock-in and knock-out models, that can be used to further advance…

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