Tag: BBMerge

BBMerge / Tadpole error correction

I’ve been using BBMerge recently to address a very specific problem: I am sequencing pooled short DNA molecules (< 400bps) using paired end reads (average length ~ 230 bps post trimming) Each molecule can be assumed to be different (i.e. contains sequence differences – substitutions & indels – with respect…

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Getting nucleotide frequencies per position

Getting nucleotide frequencies per position 0 Hi, I am very new to bioinformatics and was given the task to analyze some total RNA sequencing results. So far I have converted my .fastaq files to indexed .bam using samtools index. I found the following in literature: # Generate nucleotide counts per…

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