Tag: bin

Liberate Bio is Bringing Precision Delivery to Genetic Medicines

The development of efficient delivery technologies has lagged behind significant advancements in nucleic acid therapeutics, such as antisense oligonucleotids (ASOs), short interfering RNA (siRNA), mRNA therapeutics, and genome editors. There must be a renaissance in genetic medicine delivery for these strategies to realize their full potential. Increasingly, the greatest hope…

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Error when Installing BioConductor Package (‘affy’)

Hi, when I try to install Bioconductor package ‘affy’ Experience below error\ May I know how do I fix this? I’m currently using R4.2.2 on MacBook Air, M1\ R version 4.2.2 (2022-10-31) — “Innocent and Trusting”\ Copyright (C) 2022 The R Foundation for Statistical Computing\ Platform: aarch64-apple-darwin20 (64-bit) BiocManager::install(“affy”) ‘getOption(“repos”)’…

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r – Cannot Install maftools into BiocManager due to RSQLite

I am a relatively new R-user. I am currently working on trying to read .maf files via the maftools package released by Bioconductor. I am following the instructions as posted on the Bioconductor website (www.bioconductor.org/packages/devel/bioc/vignettes/maftools/inst/doc/maftools.html#4_Installation), however, everytime I attempt to run the installation, it gets stuck on RSQLite and I…

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Getting information on CRAM files from headers inside the files

Getting information on CRAM files from headers inside the files 1 Hello. I wish to know if one can find the following information in CRAM files’ headers: 1) Whether or not sequencing data in CRAM files is from WGS or WES, and if so, where? and 2) In case one…

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Bug#1024835: python-pauvre: ftbfs with biopython 1.80

Source: python-pauvre Version: 0.2.3-1 Severity: important Tags: ftbfs Dear Maintainer, python-pauvre fails its build time test suite when built against biopython 1.80 in experimental but builds successfully against biopython 1.79. The relevant part of the log in case of failure shows: I: pybuild base:240: cd /<<PKGBUILDDIR>>/.pybuild/cpython3_3.10_pauvre/build; python3.10 -m unittest discover -v test_normal_plotting_scenario (pauvre.tests.test_synplot.libSeq_test_case) This…

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lazy loading failed, unable to load shared object rtracklayer.so

Hello! I am working on analyzing a dataset I created with the 10x Chromium Single Cell Multiome kit. In order to add gene annotation to the ATAC data, I am trying to install and use the “EnsDb.Mmusculus.v79” and “BSgenome.Mmusculus.UCSC.mm10” packages with bioconductor. The same ERROR has come up repeatedly whenever…

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Rsubread featureCounts outputs dozens of temp files, no counts

Rsubread featureCounts outputs dozens of temp files, no counts 1 @83165de1 Last seen 16 hours ago United States Hello, I am having trouble getting an output file in Rsubreads using featureCounts. I want to set up my data to run analysis of differential expresssion in EdgeR. I’m running about 40…

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java – I am unable to execute the fastqc command in HPC cluster

I am trying to run fastqc for fastq.gz files in RNA-seq Data analysis and I have written a PBS script. However, I am encountering the following error Can’t exec “java”: No such file or directory at /home/paksls/Atreya/software/FastQC/fastqc line 307 I have written the following script: #!/bin/bash #PBS -l nodes=1:ppn=6,pmem=10240MB,walltime=04:00:00 #PBS…

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How to install Biopython in Python

Table of Contents # Install Biopython on Windows Install Biopython on macOS or Linux Install Biopython in Visual Studio Code Install Biopython in PyCharm Install Biopython in Anaconda Install Biopython in Jupyter Notebook Install biopython on Windows # To install the biopython module on Windows: Type CMD in the search…

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Multi-level cellular and functional annotation of single-cell transcriptomes using scPipeline

Software Figure preparation: CorelDRAW x8 (Corel); Bioinformatic analyses: R v 4.0.3 (R Foundation for Statistical Computing). Computational resources Analyses were run on a desktop computer with an Intel Core i9-10900L CPU (3.70 GHz, 10 cores, 20 threads) with 120 GB RAM running Windows 10 Pro (v21H2). Data preprocessing scRNA-seq data sets…

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r – monocle3 installation issues

I have R 4.2.1 and the latest R studio build 554 on a macOS Monterey. I am trying to install monocle3 using the following : BiocManager::install(‘monocle3’) I get a really long error message BiocManager::install(“monocle3”) ‘getOption(“repos”)’ replaces Bioconductor standard repositories, see ‘?repositories’ for details replacement repositories: CRAN: cran.rstudio.com/ Bioconductor version 3.15…

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Phenotypic plasticity and genetic control in colorectal cancer evolution

Sample preparation and sequencing The method of sample collection and processing is described in a companion article (ref. 23). Sequencing and basic bioinformatic processing of DNA-, RNA- and ATAC-seq data are included there as well. Gene expression normalization and filtering The number of non-ribosomal protein-coding genes on the 23 canonical chromosome pairs…

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Ete3 build – ValueError: Error parsing Phyml result

Ete3 build – ValueError: Error parsing Phyml result 0 Hello, I am using ete3 build to build a phylogeny of my target species. I ran the following command: ete3 build -a eteinput.faa –cogs outfile.tab -o test1_results -m sptree_fasttree_85 -w full_modeltest_bootstrap –clearall –cpu 20 I am getting the following error: Traceback…

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r – Cannot install bioconductor DESeq2 package on M1 mac … any advice?

This is where the installation failed. I tried installing DESeq2 on “R Studio Cloud” and it worked. I am assuming this is a mac issue. If anyone knows what to do I would very much appreciate it! Very new to R so anything will help. make: /opt/R/arm64/bin/gfortran: No such file…

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Ex Jupyter Notebook With Code Examples

Ex Jupyter Notebook With Code Examples This article will demonstrate via examples how to resolve the Ex Jupyter Notebook error . pip install voila As we have seen, a large number of examples were utilised in order to solve the Ex Jupyter Notebook problem that was present. What are the…

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sra-toolkit not working

sra-toolkit not working 2 I downloaded sra-toolkit from sra website for 64bit Windows. After extracting I tested it from bin folder: sra-toolkit\bin> fastq-dump –stdout -X 2 SRR390728 but it throws error: 2015-12-15T09:54:40 fastq-dump.2.5.5 err: item not found while constructing within virtual database module – the path ‘SRR390728’ cannot be opened…

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Quarto project does not appear even after updating RStudio

RStudio latest version is compatible with macOS version 10.15+ however my OS version is 10.14.6. The latest RStudio version that my OS can support is 2021.09.0 which does not happen to come with Quarto. (Quarto is available in 2022 releases). I upgraded R to version 4.2.1 however, that did not…

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Inferring and perturbing cell fate regulomes in human brain organoids

Experimental methods Stem cell and organoid culture We used six human iPS cell lines (Hoik1, Wibj2, Kucg2 from the HipSci resource47; 409B2 from the RIKEN BRC cell bank; 01F49i-N-B7 (B7) from Institute of Molecular and Clinical Ophthalmology Basel; and WTC from the Allen Institute) and three human ES cell lines (H1-PAX6YFP…

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Bedtools Bam To Bed With Code Examples

Bedtools Bam To Bed With Code Examples With this article, we’ll look at some examples of how to address the Bedtools Bam To Bed problem . bedtools bamtobed [OPTIONS] -i <BAM> As we have seen, a large number of examples were utilised in order to solve the Bedtools Bam To…

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‘info jupyterhub’ displays the manpage although it suggests ‘info’ to get the full doc

Package: jupyterhub Version: 2.0.0+ds1-2 Severity: normal X-Debbugs-Cc: gkni…@australdx.fr Dear Maintainer, ‘man jupyterhub’ ends with: > The full documentation for jupyterhub is maintained as a Texinfo manual. If the info and jupyterhub programs are properly installed at your site, the command > info jupyterhub > should give you access to the complete manual. although…

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Running lammps on a cluster – LAMMPS Installation

Hello all,I have created a Linux cluster with ssh and NFS. I have installed lammps in the NFS folder. I have verified that the cluster works properly with openmpi and the communication is good. But when I run lammps, after reading the data file it gets stuck on the first…

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GPU inference doesn’t work when importing open3d

Thanks for your error report and we appreciate it a lot. Checklist I have searched related issues but cannot get the expected help. I have read the FAQ documentation but cannot get the expected help. The bug has not been fixed in the latest version. Describe the bug The GPU…

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Optrode Array for Simultaneous Optogenetic Modulation and Electrical Neural Recording

Here, we present the fabrication method of an optrode system with optical fibers for light delivery and an electrode array for neural recording. In vivo experiments with transgenic mice expressing channelrhodopsin-2 show the feasibility of the system for simultaneous optogenetic stimulation and electrophysiological recording. This protocol includes fabricating on optrode…

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iPSCs derived from infertile men carrying complex genetic abnormalities can generate primordial germ-like cells

Patients and controls The patient 1 was 38 years old and consulted for infertility after he and his partner had been trying to conceive for 2 years. The patient was the first child of unrelated parents, and he had four brothers and five sisters whose fertility status could not be determined…

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Running parfor on SLURM limits cores to 1 – MATLAB Answers

Hello, I’m trying to run some parallelized code (through parfor) on a university high performance cluster. In order to make sure parallelization is working correctly, I set up a single node with 32 cores via “srun –pty -t 00:30:00 -n 32 -N 1 /bin/bash -l”, which I verify does start…

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Not enough memory on RTX 3090 to train ViTDet?

If you do not know the root cause of the problem, please post according to this template: Instructions To Reproduce the Issue: I’m trying to train an instance segmentation ViTDet model with a custom and relatively small dataset (6000 images of 640×480) I’m using Windows 10 and RTX 3090. I’m…

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Bash script to automate htseq-count

Hi everyone- I am trying to write a script to automate htseq-count on a large number of samples. The script runs but then throws the following error: “Please provide 2 arguments”. Does anyone see something obvious I am missing: #!/bin/bash for samples in *.sam do gtf = “Galaxy135-\[Escherichia_coli_str_k_12_substr_mg1655.GCA_000005845.2.29.gtf\].gtf” echo $sample …

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Cannot setup Jupyterhub+Jupyterlab to run as a service on Ubuntu machine

I am supposed to set up Jupyterhub Server for my team at work The idea is that everybody gets a JupyterLab interface in the browser when they access http://ourserverip:<port>. I’ve been following this link for the instructions. I literally followed every step at the exact same order and fail at…

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YOLOv7 Pose estimation using OpenCV, PyTorch

from google.colab import drive drive.mount(“/content/drive”) git clone github.com/RizwanMunawar/yolov7-pose-estimation.git cd yolov7-pose-estimation ### For Linux Users python3 -m venv psestenv source psestenv/bin/activate ### For Window Users python3 -m venv psestenv cd psestenv cd Scripts activate cd .. cd .. pip install –upgrade pip pip install -r requirements.txt python pose-estimate.py #if you want…

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mapping – STAR error in snakemake pipeline: “EXITING because of FATAL ERROR: could not open genome file”

I’m trying to use a 2 pass STAR mapping strategy (also explained here informatics.fas.harvard.edu/rsem-example-on-odyssey.html), but I’m getting an error. I’ve read through this page [https://github.com/alexdobin/STAR/issues/181] and I have a similar issue, but the discussed solutions don’t seem to help. Perhaps this is more a snakemake issue rather than a STAR…

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Multidrug resistance microbial therapy | VMRR

Introduction Antimicrobials are the most important and useful therapeutic discovery in the history of medicine. It allows living beings to survive the microbial disease, enhances invasive surgical procedures, ensures animal health, and protects the food chain since 1928 after the discovery of penicillin. However, the indiscriminate use of antibiotics and…

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Setting up Aspera Connect (ascp) on Linux and macOS

This tiny tutorial cover setting up Aspera Connect (binary is called ascp) which might be used to download sequencing data, e.g. with download links provided by sra-explorer.info, see also sra-explorer : find SRA and FastQ download URLs in a couple of clicks Setting up Aspera Connect is simple and was…

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I cannot install “microbiome”

Hi there, i cannot install package “microbiome” in R (ver 4.2.1), when i input the code, it seems OK, BiocManager::install(“microbiome”, force = TRUE) ‘getOption(“repos”)’ replaces Bioconductor standard repositories, see ‘?repositories’ for details replacement repositories: CRAN: cran.rstudio.com/ Bioconductor version 3.15 (BiocManager 1.30.18), R 4.2.1 (2022-06-23 ucrt) Installing package(s) ‘microbiome’ trying URL…

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Cornell Virtual Workshop: Execution: idev (at TACC)

TACC also offers their idev command (for interactive development) as a convenient way to initiate interactive work on their compute nodes, via Slurm. It works very much like srun and even takes many of the same options. But by making a few assumptions, idev shortens the path for you…

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GROMACS Installation Problem in The Grid Computer – User discussions

GROMACS version: 2021.2GROMACS modification: No Hello all, I am new to GROMACS. I wanted to install it on a grid computer servers supported by the government and I’ve done step by step instructions in the following link (The language of the instructions is in Turkish but you may find the…

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Potential file is missing an entry – LAMMPS General Discussion

Dear all, I am using LAMMPS to simulate some thermodynamic properties of b-SiGe. I found the potential file in the official github repository for LAMMPS . The first part of my code is this ############################################################### #1 general units metal dimension 3 atom_style atomic neighbor 0.3 bin #2 structure boundary p…

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[gmx-users] dssp

Hi,I have a problem with dssp programme.I have the programme in /home/m/DSSP. When I writeexport DSSP=/usr/local/binand then I check /usr/local/bin I couldn’t see dssp.How can I handle this problem. I have caheceked gromacs website export DSSP=/path/to/dssp setenv DSSP /path/to/dssp commands are written.Could you write export DSSP=/path/to/dssp command according to me….

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[Buildroot] [git commit branch/2022.02.x] package/libmodsecurity: needs dynamic library with libcurl and mbedtls

[Buildroot] [git commit branch/2022.02.x] package/libmodsecurity: needs dynamic library with libcurl and mbedtls – Peter Korsgaard From: Peter Korsgaard <peter@korsgaard.com> To: buildroot@buildroot.org Subject: [Buildroot] [git commit branch/2022.02.x] package/libmodsecurity: needs dynamic library with libcurl and mbedtls Date: Tue, 19 Jul 2022 18:35:54 +0200 [thread overview] Message-ID: <20220719162323.01D1283DF5@busybox.osuosl.org> (raw) commit: git.buildroot.net/buildroot/commit/?id=2d021a7e7163d13b604d508602288e7190ab63dc branch: git.buildroot.net/buildroot/commit/?id=refs/heads/2022.02.x

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[ccp4bb] Vacancy for EMDB Team Leader (EMBL Faculty position)

Dear colleagues, EMBL-EBI is looking to recruit a new Team Leader (EMBL Faculty position) to take charge of the Electron Microscopy Data Bank (EMDB; emdb-empiar.org), one of the three core archives of the Worldwide Protein Data Bank organisation (wwPDB; wwpdb.org). In the past two years, EMDB has doubled in size…

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Transition from Woody with Ubuntu 18.04 and Torque to Woody-NG with AlmaLinux8 and Slurm

2022-07-17 Valued Tier3 HPC users of NHR@FAU, as briefly announced in the HPC Cafe in June, we now started with switching from Woody with Ubuntu 18.04 and Torque to Woody-NG (“Woody Next Generation”) with AlmaLinux8 as operating system and Slurm as batch system. Woody-NG uses the same operating system as…

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Mapping reads using kallisto – rna seq analysis

Mapping reads using kallisto – rna seq analysis 0 Hi, I’m trying to map reads to a reference genome using kallisto for rna seq analysis with terminal on mac and the following command keeps loading for hours and won’t run. I’m not exactly sure where I’ve gone wrong. kallisto index…

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Metagenome Assembly

Generally speaking, longer contigs mean better assembly. It is much better to have a small number of long contigs than a large number of short contigs, even if the number of bases is identical between the two. Longer contigs can be binned more reliably because they have stronger signal. Consider…

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Within analysis, low-coverage whole-genome sequencing out of cfDNA was held to examine blood plasma away from patients with spine metastasis

Within analysis, low-coverage whole-genome sequencing out of cfDNA was held to examine blood plasma away from patients with spine metastasis An analysis pipe is made and you will verified to evaluate the brand new CNV condition within the cfDNA, in order to determine whether brand new CIN score, that has…

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Alternatives and detailed information of Prsice

PRSice (pronounced ‘precise’) is a software package for calculating, applying, evaluating and plotting the results of polygenic risk scores (PRS). PRSice can run at high-resolution to provide the best-fit PRS as well as provide results calculated at broad P-value thresholds, illustrating results corresponding to either, can thin SNPs according to…

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autopkgtest needs update for new version of python-decorator)

Hi, I’d like to point out to this serious bug. Steffen, would you mind koordinating (not doing alone) an upgrade of the qiime infrastructure to make sure everything will go smoothly? Kind regards Andreas. Am Thu, Jun 30, 2022 at 11:30:21AM +0200 schrieb Paul Gevers: > Source: qiime > Version:…

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Three families of Asgard archaeal viruses identified in metagenome-assembled genomes

Liu, Y. et al. Expanded diversity of Asgard archaea and their relationships with eukaryotes. Nature 593, 553–557 (2021). CAS  PubMed  Google Scholar  Dombrowski, N., Teske, A. P. & Baker, B. J. Expansive microbial metabolic versatility and biodiversity in dynamic Guaymas Basin hydrothermal sediments. Nat. Commun. 9, 4999 (2018). PubMed  PubMed…

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Unexpected behaviour during hybrid Monte-Carlo simulations (ReaxFF) – LAMMPS Beginners

T.Dem June 25, 2022, 5:41pm #1 Dear LAMMPS users, I am trying to run Monte Carlo simulations using reaxff to study the oxygen coverage on a Pt nanoparticle. I defined my oxygen reservoir using the chemical potential definition for a perfect gas. Initially, the system is very small (350 atoms)…

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Single-cell analyses define a continuum of cell state and composition changes in the malignant transformation of polyps to colorectal cancer

Mapping molecular changes across malignant transformation We generated single-cell data for 81 samples collected from eight FAP and seven non-FAP donors (Fig. 1a and Supplementary Tables 1 and 2). For each tissue, we performed matched scATAC-seq and snRNA-seq (10x Genomics). We obtained high-quality single-cell chromatin accessibility profiles for 447,829 cells…

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ERROR No such moleculetype NA – User discussions

GROMACS version:GROMACS modification: Yes/No I got this errorwhen I run the line: gmx grompp -f em.mdp -c solv_ions.gro -p topol.top -o em.tpr -maxwarn 10in the topol.top there is 3 NA atoms 🙂 GROMACS – gmx grompp, 2020.1-Ubuntu-2020.1-1 (-: GROMACS is written by: Emile Apol Rossen Apostolov Paul Bauer Herman J.C….

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Convert ONNX RuntimeError: tin_shift_forward_impl: implementation for device cpu not found.

when convert tin to onnx meet this issue: RuntimeError: tin_shift_forward_impl: implementation for device cpu not found. env:sys.platform: linuxPython: 3.8.10 (default, Jun 2 2021, 10:49:15) [GCC 9.4.0]CUDA available: TrueGPU 0,1: Tesla T4CUDA_HOME: /usr/local/cudaNVCC: Cuda compilation tools, release 11.4, V11.4.120GCC: x86_64-linux-gnu-gcc (Ubuntu 9.3.0-17ubuntu1~20.04) 9.3.0PyTorch: 1.9.1+cu111PyTorch compiling details: PyTorch built with: GCC 7.3…

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main-i386-default][science/cp2k] Failed for cp2k-9.1.0 in configure

You are receiving this mail as a port that you maintain is failing to build on the FreeBSD package build server. Please investigate the failure and submit a PR to fix build. Maintainer: y…@freebsd.org Log URL: beefy17.nyi.freebsd.org/data/main-i386-default/p429f3de9bdcd_s2573e6ced9/logs/cp2k-9.1.0.log Build URL: beefy17.nyi.freebsd.org/build.html?mastername=main-i386-default&build=p429f3de9bdcd_s2573e6ced9 Log: =>> Building science/cp2k build started at Sat Jun 4…

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Go Echo API Server for openapi

A hello world service. Overview This server was generated by the openapi-generator project. By using the OpenAPI-Spec from a remote server, you can easily generate a server stub. To see how to make this your own, look here: README API version: 1.0 Build date: 2022-05-28T21:42:12.319613+10:00[Australia/Sydney] export GO_POST_PROCESS_FILE=”/usr/local/bin/gofmt -w” openapi-generator generate…

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RPKM threshold estimation – SEQanswers

Dear All, I have a doubt in the calculation of False postitive rate while checking for FPKM threshold in a RNAseq experiment. Following the method previously published (www.ploscompbiol.org/article/…l.pcbi.1000598). I am not getting desired results. I followed the method as mentioned the publication Reads were mapped to Ensembl genes (blue) and…

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fix checkpatch.pl struct should normally be const — DRI Development

Hi Uri, Thank you for the patch! Yet something to improve: [auto build test ERROR on staging/staging-testing] url: github.com/intel-lab-lkp/linux/commits/Uri-Arev/staging-fbtft-fix-checkpatch-pl-struct-should-normally-be-const/20220520-012948 base: git.kernel.org/pub/scm/linux/kernel/git/gregkh/staging.git 4d0cc9e0e53e9946d7b8dc58279c62dfa7a2191b config: arm64-randconfig-r011-20220519 (download.01.org/0day-ci/archive/20220520/202205200821.nJQ0IfFt-lkp@xxxxxxxxx/config) compiler: clang version 15.0.0 (github.com/llvm/llvm-project e00cbbec06c08dc616a0d52a20f678b8fbd4e304) reproduce (this is a W=1 build): wget raw.githubusercontent.com/intel/lkp-tests/master/sbin/make.cross -O ~/bin/make.cross chmod +x ~/bin/make.cross # install arm64 cross compiling tool…

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How do I install biopython in anaconda?

Package maintainers recommend using (in the terminal): conda install -c conda-forge biopython We deliberately recommend using Biopython from the conda-forge channel, as this is usually up to date and covers Windows, Mac OS X and Linux. The default Conda channel does have Biopython, but is often out of date. biopython.org/wiki/Packages…

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Unicode Characters in ggplot2 PDF Output

As Ben suggested, cairo_pdf() is your friend. It also allows you to embed non-postscript fonts (i.e. TTF/OTF) in the PDF via the family argument (crucial if you don’t happen to have any postscript fonts that contain the glyphs you want to use). For example: library(ggplot2) cairo_pdf(“example.pdf”, family=”DejaVu Sans”) qplot(Sepal.Length, Petal.Length,…

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Description, Programming Languages, Similar Projects of Python-for-Text-Classification

Python for Text Classification with Machine Learning in Python 3.6. Installation Guide Start your environment by picking either pipenv (recommended) or virtualenv. Simple guides are below. Using pipenv Initialize pipenv (setup guide): cd path/to/your/dev/folder mkdir text-classify cd text-classify pipenv install –three After installation of pipenv works, just activate it (same…

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SchedMD Slurm privilege escalation | CVE-2022-29501

NAME SchedMD Slurm privilege escalation Platforms Affected:SchedMD Slurm 20.11.6SchedMD Slurm 20.02.6 Risk Level:9.8 Exploitability:Unproven Consequences:Gain Privileges DESCRIPTION SchedMD Slurm could allow a remote attacker to gain elevated privileges on the system, caused by improper access control in a network RPC handler in the slurmd daemon used for PMI2 and PMIx…

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Distributed training on slurm cluster – distributed

chinmay5 (Chinmay5) April 29, 2022, 12:48pm #1 Sorry for the naive question but I am confused about the integration of distributed training in a slurm cluster. Do we need to explicitly call the distributed.launch when invoking the python script or is this taken care of automatically? In other words, is…

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CircRNA and miRNA expression profiles during remote ischemic postconditioning attenuate brain ischemia/reperfusion injury

. 2022 Apr 19;S0361-9230(22)00098-3. doi: 10.1016/j.brainresbull.2022.04.006. Online ahead of print. Chun-Yan Li  1 , Wei Ma  2 , Kuang-Pin Liu  2 , Jin-Wei Yang  3 , Xian-Bin Wang  2 , Zhen Wu  3 , Tong Zhang  3 , Jia-Wei Wang  3 , Wei Liu  2 , Jie Liu  2 , Yu Liang  2 , Xing-Kui Zhang  2 , Jun-Jun Li  2 , Jian-Hui…

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Calculate scores using certain method by comparing a bedGraph file from treatment and a file from control representing local bias

bdgcmp(1) Calculate scores using certain method by comparing a bedGraph file from treatment and a file from control representing local bias SYNOPSIS bdgcmp <-t TREATMENT.BEDGRAPH> <-c CONTROL.BEDGRAPH> <-o OUTPUT.BEDGRAPH> [-m METHOD] DESCRIPTION Calculate scores using certain method by comparing a bedGraph file from treatment and a file from control representing…

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Cannot compile any CUDA program – CUDA Programming and Performance

I am getting the following error when I run sample programs on vs 2022.My details:Windows 11 x64GTX 1650i7 9gen8 gb ram Severity Code Description Project File Line Suppression State Error MSB3721 The command C:\Program Files\NVIDIA GPU Computing Toolkit\CUDA\v11.6\bin\nvcc.exe -gencode=arch=compute_35,code=\sm_35,compute_35\ -gencode=arch=compute_37,code=\sm_37,compute_37\ -gencode=arch=compute_50,code=\sm_50,compute_50\ -gencode=arch=compute_52,code=\sm_52,compute_52\ -gencode=arch=compute_60,code=\sm_60,compute_60\ -gencode=arch=compute_61,code=\sm_61,compute_61\ -gencode=arch=compute_70,code=\sm_70,compute_70\ -gencode=arch=compute_75,code=\sm_75,compute_75\ -gencode=arch=compute_80,code=\sm_80,compute_80\ -gencode=arch=compute_86,code=\sm_86,compute_86\ –use-local-env -ccbin…

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Conducting Respiratory Oscillometry in an Outpatient Setting

Respiratory telemetry is a different modality of the pulmonary function testing that is now increasingly used in the clinical and research setting. Oscillometry is conducted through tighter breathing with three acceptable measurements, and can be performed with minimal contraindications. The main advantage of respiratory oscillometry is that it requires minimal…

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Issues with searching Swissprot #25

Eddykay310 Hi @cruizperez Please help me understand the problem here and how I can fix it. I have successfully generated my DBs but I get this error during analysis. The .dmnd files do not exist in the folders as the error says but I don’t know how I can generate…

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[slurm-users] Issues with pam_slurm_adopt

Hi, I have an issue with pam_slurm_adopt when I moved from 21.08.5 to 21.08.6. It no longer works. When I log straight to the node with root account : Apr 8 19:06:49 magi46 pam_slurm_adopt[20400]: Ignoring root user Apr 8 19:06:49 magi46 sshd[20400]: Accepted publickey for root from 172.16.0.3 port 50884 ssh2:…

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Problem with phylotype(taxonomy=final.taxonomy) – Commands in mothur

Sam92 April 5, 2022, 3:07am #1 Hello, I want to bin my sequences in to phylotypes according to their classification. I used the following command, phylotype(taxonomy=year1.trim.pcr.trim.good.unique.good.filter.unique.precluster.pick.pick.opti_mcc.0.03.pick.0.03.cons.taxonomy) After running this, it crashed mothur instantly. I also tried running it on servers and got the same results.I am using mothur v.1.45.2 Please…

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cgview: Can’t locate Bio/SeqIO.pm in @INC

Tried two new images quay.io/biocontainers/cgview:1.0–5 quay.io/biocontainers/cgview:1.0–4 Same issue: Digest: sha256:c824eff236de755b06805a02df35f58c67d3aa4c73c09fb7dce3742336ab57f3 Status: Downloaded newer image for quay.io/biocontainers/cgview:1.0–4 Can’t locate Bio/SeqIO.pm in @INC (you may need to install the Bio::SeqIO module) (@INC contains: /usr/local/lib/site_perl/5.26.2/x86_64-linux-thread-multi /usr/local/lib/site_perl/5.26.2 /usr/local/lib/5.26.2/x86_64-linux-thread-multi /usr/local/lib/5.26.2 .) at /usr/local/bin/cgview_xml_builder.pl line 17. BEGIN failed–compilation aborted at /usr/local/bin/cgview_xml_builder.pl line 17. Read more here:…

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Problem with Python environment and Slurm (srun/sbatch)

I had a similar issue with Slurm Version 20.11.7I had a virtual environment created with the systems python3, which was Python 3.6.8When activating the venv on the logging-node calling an installed module worked fine, but from within the following shell script for example it did not and resulted in ModuleNotFound:…

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Loading environment modules on a cluster from my python script

I recently ran into this. A simple way around this would be to include the dependencies before the command you want, and separate them with a semi-colon cmd = ‘module load intel/2016.4; “gromacs command”‘ p = sub.Popen(cmd, shell=True, stderr = sub.STDOUT, stdout = sub.PIPE).communicate()[0] where “gromacs command” represents however you…

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Mapped reference id is not an id of the genome file genome_nowhitespace.fa

miRDeep2: Mapped reference id is not an id of the genome file genome_nowhitespace.fa 1 Hi everyone, I’m trying to run nf-co.re/smrnaseq pipeline and I’m having a problem with mirdeep2. Command: nextflow run nf-core/smrnaseq -profile ijcluster –input /home/794_both.fastq.gz –outdir /home/results –genome GRCh38 –protocol qiaseq –mature mirbase.org/ftp/CURRENT/mature.fa.gz –hairpin mirbase.org/ftp/CURRENT/hairpin.fa.gz Error message: Command…

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A multi-omics approach to elucidate the mechanisms of action of a dietary muramidase administered to broiler chickens

Gadde, U., Kim, W. H., Oh, S. T. & Lillehoj, H. S. Alternatives to antibiotics for maximizing growth performance and feed efficiency in poultry: a review. Animal Health Res. Rev. 18, 26–45 (2017). CAS  Google Scholar  Smits, C. H. M., Li, D., Patience, J. F. & den Hartog, L. A….

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Enable MBEDTLS debugging Nordic provided security backend (for CoAP Secure via OpenThread on nRF5340) – Nordic Q&A – Nordic DevZone

Goal Hi guys, is there an option to enable MBEDTLS debugging as with the CONFIG_MBEDTLS_DEBUG_LEVEL=4 for the MBEDTLS_BUILTIN? I am trying to setup a DTLS client based in order to establish a CoAP Secure Session via Openthread to a Borderrouter and I am struggling in the handshake process. It would…

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/usr/share/sagemath/bin/sage-python: line 2: 96052 Killed sage -python “$@”

It looks the Python gets killed during running a long loop and task didn’t finish. [354, -70, 185, -239] 3.1415926589061544881 j = -48 /usr/share/sagemath/bin/sage-python: line 2: 96052 Killed sage -python “$@” I hope to upload the run-file here… But I just paste it below: eps=2^-52 digits=[2,3,5,7] ndig=len(digits) eran=[ceil(30*log(1/eps)/log(digits[i])) for i…

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#1008368 – pigx-rnaseq: FTBFS: configure: error: R package rmarkdown not found.

#1008368 – pigx-rnaseq: FTBFS: configure: error: R package rmarkdown not found. – Debian Bug report logs Reported by: Lucas Nussbaum <lucas@debian.org> Date: Sat, 26 Mar 2022 21:12:04 UTC Severity: serious Tags: bookworm, ftbfs, sid Found in version pigx-rnaseq/0.0.19-2 Reply or subscribe to this bug. Toggle useless messages Report forwarded to…

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Bioconductor installation problems

Dear Friends, I have been having considerable difficulty doing various package related bioconductor actions. Here is my system specifications: ======================================================================= macOS Monterey Version: 12.3 (21E230) MacBook Pro (Retina, 13-inch, Early 2015) Memory: 16 GB 1867 MHz DDR3 RStudio 2021.09.0 Build 351 R version 4.1.2 (2021-11-01) — “Bird Hippie” Copyright (C)…

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#1007974 – polyml: autopkgtest regression on amd64: relocation in read-only section `.text’

#1007974 – polyml: autopkgtest regression on amd64: relocation in read-only section `.text’ – Debian Bug report logs Reply or subscribe to this bug. Toggle useless messages Report forwarded to debian-bugs-dist@lists.debian.org, Debian Science Maintainers <debian-science-maintainers@lists.alioth.debian.org>:Bug#1007974; Package src:polyml. (Sat, 19 Mar 2022 20:39:04 GMT) (full text, mbox, link). Acknowledgement sent to Paul…

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Nvt.mdp killed in 7 min – User discussions

GROMACS version:2022+cp2k interface basically, my system is small but I couldn’t start nvt job. Can you I help me, what I am doing wrong?Thank you in advance I am taking that error : Command line:gmx_cp2k mdrun -s nvt.tpr -v -deffnm nvt Compiled SIMD: AVX_256, but for this host/run AVX2_256 might…

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“No such file or directory: ‘test.xml”

Biopython NcbiblastpCommandline not working: “No such file or directory: ‘test.xml” 0 from Bio.Blast.Applications import NcbiblastpCommandline blastp=r”C:\NCBI\blast-BLAST_VERSION+\bin\blastp.exe” blastp_cline = NcbiblastpCommandline(blastp, query=r”C:/NCBI/blast-BLAST_VERSION+/bin/test.fasta”, db=r’C:/NCBI/blast-BLAST_VERSION+/bin/bos_protein.fasta’, outfmt=5, evalue=0.00001, out=r”C:/NCBI/blast-BLAST_VERSION+/bin/test.XML”) blastp_cline from Bio.Blast import NCBIXML with open(“test.XML”) as result_handle: E_VALUE_THRESH=0.01 blast_records = NCBIXML.parse(result_handle) blast_record = NCBIXML.read(result_handle) for alignment in blast_record.alignments: for hsp in alignment.hsps: if hsp.expect…

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Debian — File list of package minimap2/bookworm/i386

/usr/bin/minimap2 /usr/share/doc-base/minimap2.minimap2 /usr/share/doc/minimap2/changelog.Debian.gz /usr/share/doc/minimap2/copyright /usr/share/doc/minimap2/minimap2.pdf /usr/share/doc/minimap2/run-unit-test /usr/share/doc/minimap2/test/MT-human.fa.gz /usr/share/doc/minimap2/test/MT-orang.fa.gz /usr/share/doc/minimap2/test/q-inv.fa.gz /usr/share/doc/minimap2/test/q2.fa /usr/share/doc/minimap2/test/t-inv.fa.gz /usr/share/doc/minimap2/test/t2.fa /usr/share/doc/minimap2/test_script /usr/share/man/man1/minimap2.1.gz Read more here: Source link

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#1007732 – ccache: autopkgtest regression: cannot execute ‘cc1plus’

#1007732 – ccache: autopkgtest regression: cannot execute ‘cc1plus’ – Debian Bug report logs Reply or subscribe to this bug. Toggle useless messages Report forwarded to debian-bugs-dist@lists.debian.org, Joel Rosdahl <joel@debian.org>:Bug#1007732; Package src:ccache. (Tue, 15 Mar 2022 20:27:05 GMT) (full text, mbox, link). Acknowledgement sent to Paul Gevers <elbrus@debian.org>:New Bug report received…

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A-Prot: protein structure modeling using MSA transformer | BMC Bioinformatics

Benchmarking contact prediction First, we benchmarked the long-range contact prediction performance of A-Prot using the FM and FM/TBM targets of CASP13 [24]. The benchmark results show that the performance of our model outperforms that of the existing methods (Table 1). We compared the precision of our model’s top L/5, L/2,…

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How do I get SLURM to allocate jobs to all CPUs

I’m trying to run an array job with the configuration below. My nodes have 4 cpu, but the scheduler is only allocating two jobs per node. How can I get it to allocate four jobs per node? When I add #SBATCH –ntasks-per-node=4, it’s even worse – I get one task…

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Where is numpy, scipy, etc. for Python 3.7 ??

I have Sagemath installed on my Mac with Python versions 2.7 and 3.7 in the package. I’m running sage/python as scripts. For Python 2.7 I use the shebang, #!/Applications/SageMath/local/bin/python2.7 And the code executes correctly including an “import numpy as NP” line. When I switch to Python 3.7 I use the…

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[slurm-users] Performance with hybrid setup

Hello, I’m performing some tests (CPU-only systems) in order to compare MPI versus hybrid setup. The system is running OpenMPIv4.1.2 so that a job submission reads:       mpirun -np 48 foo.exe or       export OMP_NUM_THREADS=8       mpirun -np 6 foo.exe In our system, the latter runs slightly faster (about 5…

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Install Jupyter Notebook on Debian 11 Bullseye Linux

Tutorial to learn the steps involve in the installation of Jupyter Notebook on Debian 11 Bullseye using command terminal. What is Jupyter Notebook? The Jupyter Project is a non-profit initiative that aims to develop and provide open-source software and open standards for interactive work. One of the most famous products…

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Crystallography, cryo-EM and AlphaFold shed light on key human antibacterial proteins

Figure 1. Model of the human UMOD/FimH interaction, assembled by combining the structural information described in the study. A, The high-mannose sugar chain (green) of the decoy modules branching out from a filament of UMOD (shades of blue) is bound by the FimH sugar-binding domain at the tip of bacterial…

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cutadapt installation location? | Code Info Park

cutadapt installation location The documentation on the installation of cutadapt is just a bit misleading, as far as I’m concerned. Cutadapt was just installed as a Python package. The cutadapt executable is probably in /Users/yc/Library/Python/2.7/bin. If that folder is already in your PATH you can just type cutadapt to run…

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Unable to run python script from bash scripts with string arguments : SLURM

I want to run it from a bash script but it is not accepting string input with spaces. It works with single words or hyphenated words. However the python command is correct and is working fine when I am running it directly from terminal. commands.txt python generate.py -p “Flower girl”…

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How to Set the Number of Bins for a Histogram in ggplot2

You can use the bins argument to specify the number of bins to use in a histogram in ggplot2: library(ggplot2) ggplot(df, aes(x=x)) + geom_histogram(bins=10) The following examples show how to use this argument in practice. Example: Set Number of Bins for Histogram in ggplot2 The following code shows how…

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[lammps-users] ERROR: Non-numeric pressure – simulation unstable – LAMMPS Mailing List Mirror

Dear Lammps users, I want to relax the wurtzite structure by SW polymorphic potentiel , but when I try to do simulation with Lammps, I got an error.I have attached the input data and the code for relaxation.LAMMPS (29 Sep 2021 – Update 1) units metal#package omp 0 neigh yes…

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ggplot2 – Multiple Relative frequency histogram in R, ggplot

Below are some basic example with the build-in iris dataset. The relative part is obtained by multiplying the density with the binwidth. library(ggplot2) ggplot(iris, aes(Sepal.Length, fill = Species)) + geom_histogram(aes(y = after_stat(density * width)), position = “identity”, alpha = 0.5) #> `stat_bin()` using `bins = 30`. Pick better value with…

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Index of /~psgendb/local/install/T-COFFEE_distribution_Version_8.14/bin/download/distributions/probcons/probcons

Name Last modified Size Description Parent Directory   –   CompareToRef.cc 2004-07-13 16:04 9.0K   Defaults.h 2004-07-13 16:04 5.4K   EvolutionaryTree.h 2004-07-13 16:04 5.9K   FileBuffer.h 2004-07-13 16:04 2.6K   FixRef.cc 2004-07-13 16:04 35K   Main.cc 2007-04-03 12:37 52K   MakeGnuPlot.cc 2007-04-03 12:37 1.5K   Makefile 2007-04-03 12:39 2.1K  …

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Nvcc fatal : A single input file is required for a non-link phase when an outputfile is specified – CUDA Programming and Performance

Hello, During I execute setup.py through pytorch, I faced the error “nvcc fatal : single input file is required for a non-link phase when an outputfile is specified” Could you help me out solving the problem? Code is like below. import osimport reimport subprocessimport sys from setuptools import setup#from skbuild…

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Accurate prediction of metagenome-assembled genome completeness by MAGISTA, a random forest model built on alignment-free intra-bin statistics | Environmental Microbiome

Steen AD, Crits-Christoph A, Carini P, DeAngelis KM, Fierer N, Lloyd KG, Cameron TJ. High proportions of bacteria and archaea across most biomes remain uncultured. ISME J. 2019;13:3126–30. PubMed  PubMed Central  Google Scholar  Goh KM, Shahar S, Chan K-G, Chong CS, Amran SI, Sani MH, Zakaria II, Kahar UM. Current…

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Efficient way of mapping UniProt IDs to representative UniRef90 IDs?

You can do this directly on UniProt: www.uniprot.org/uploadlists/ Just paste or upload your list of UniProt IDs, and select “UniProtKB AC/ID” in the “From” field and “UniParc” in the “To” field I’ve also written a script, pasted below, that can do this with some useful options: $ uniprot_map.pl -h uniprot_map.pl…

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Gmxapi gromacs 2022 error – User discussions

. /path/to/gromacs/bin/GMXRC You used the actual installation path here, right? ╰─> [124 lines of output] We can’t help without the full output. Please copy the raw text from the terminal window. Note that you can format the terminal output in the forum post either by using the </> button or…

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transcriptome – How to combine multiple .fasta files of primary assembly from Ensembl into one for sequence alignment?

I have some marmoset snRNA reads that I want to align with the reference transcriptome using cellranger. The primary assembly for marmoset is available here, which is broken down into 22 parts. However, cellranger mkref only accepts one .fa file to generate the transcriptome. I tried concatentaing all the extracted…

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python – PyTorch working in Miniconda but “not compiled with CUDA enabled” in PyCharm

I have had quite the journey trying to get PyCharm to use my GPU (NVIDIA GeForce GTX 1080 ti) when running code from this github: github.com/gordicaleksa/pytorch-neural-style-transfer After a whole lot of back and forth setting up CUDA, cuDNN etc., I have finally got PyTorch working (pretty sure) in my Miniconda…

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CHECK report for GGBase on malbec1

BioC 3.4: CHECK report for GGBase on malbec1 This page was generated on 2017-04-15 16:09:39 -0400 (Sat, 15 Apr 2017). GGBase 3.36.0VJ Carey Snapshot Date: 2017-04-14 17:17:13 -0400 (Fri, 14 Apr 2017) URL: hedgehog.fhcrc.org/bioconductor/branches/RELEASE_3_4/madman/Rpacks/GGBase Last Changed Rev: 122710 / Revision: 128728 Last Changed Date: 2016-10-17 14:45:06 -0400 (Mon, 17 Oct 2016) malbec1  Linux (Ubuntu 16.04.1 LTS) / x86_64   OK   OK  [ WARNINGS ] tokay1  Windows Server 2012 R2 Standard / x64   OK   OK   WARNINGS   OK  morelia  Mac OS X Mavericks (10.9.5) / x86_64   OK   OK   WARNINGS   OK  Summary Package: GGBase Version: 3.36.0…

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METASnake: a Snakemake workflow to facilitate…

Introduction As sequencing technology has become cheaper and more readily accessible, the need for the increased computational capacity to process these data has become apparent. In particular, high-throughput sequencing has been particularly useful when applied to the field of metagenomics. Substantial effort has been devoted to developing software and computational…

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