Tag: biorender

Dermal cell characterization by 10X scRNA-seq. a, Workflow used to obtain dermal cells of adult and aged mouse back skin. Single-cell suspensions were enriched separately for EpCAM–CD45– and CD45+ cells by FACS. Transcriptomes of sorted single cells were then analyzed by 10X scRNA-seq. For CD45+ cells, n = 7 mice for the adult…

Published Erratum . 2023 May 19;14:1216456. doi: 10.3389/fimmu.2023.1216456. eCollection 2023. Affiliations Expand Affiliations 1 Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China. 2 Cancer Institute, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China. 3 Department of Laboratory Medicine, Huadong Hospital, Fudan University, Shanghai,…

Plasma membrane expression of WT and mutant PTH1Rs The positions in the PTH1R of the three studied mutations of Eiken syndrome, E35K, Y134S and R485X, as well as the H223R mutation of JMC are shown in Fig. 1a and b. Cell surface expression of the receptors in transiently transfected HEK293-derived Gs22a…

Key Points Liver metastasis, which is highly prevalent in advanced malignancies, was associated with poor prognosis. Contemporary therapies in liver metastasis were associated with the lack of metastatic-targeting ability, significant systemic toxicities and incapability of tumor microenvironment (TME) modulations. Lipid nanoparticles (LNP)-based strategies would overcome the deficiencies of conventional approaches…

We analyzed data from a total of 2,733 participants from the GALA II16 and SAGE17 asthma case–control studies who self-identified as African American (AA; n = 757), Puerto Rican (PR; n = 893), Mexican American (MX; n = 784) or other Latino American (LA; n = 299) (Table 1 and Supplementary Table 1). The median age of the…

The infant and maternal gut microbiota are defined by different Bifidobacterium species The samples analysed in this study were collected during the MicrobeMom study (ISRCTN5302301420,21), these included samples from the maternal stool, vagina, oral cavity, and breast milk in addition to infant stool (Table 1; Fig. 1a). From the 160 participants recruited,…

Human DNA can be sequenced from small amounts of water, sand and air in the environment to potentially extract identifiable information like genetic lineage, gender and health risks, according to our new research. Every cell of the body contains DNA. Because each person has a unique genetic code, DNA can…

Inference of HLA-I tumor status with LILAC. a, Representation of the six immune escape pathways considered in the present study alongside their associated genes (adapted from ‘MHC class I and II pathways’, by BioRender.com). The genes considered for each immune escape pathway are depicted in gray. b, Left, workflow of…

Inference of HLA-I tumor status with LILAC Inference of the correct HLA-I tumor status is fundamental to identifying GIE alterations (Fig. 1a), to estimate the neoepitope repertoire and burden and to predict the response to immune checkpoint inhibitors14,15 (ICIs). We have developed LILAC, a framework that performs HLA-I typing for…

Figure 1 shows the detailed workflow of the study. Fig. 1 General overview of the study. Briefly, MPXV was isolated from a skin lesion and then was used to infect CV-1 cells. After the designated infection times, total RNA was isolated and sequenced using direct cDNA sequencing protocol on ONT’s MinION platform….

1. Introduction Peptides are employed for a multitude of therapeutic approaches and can be obtained from the native organ or tissue in which they are produced as well as through recombinant methods where nucleic acids encoding a candidate gene(s) are delivered to and expressed in cells in vitro. As an…

Cis-acting elements in the 3ʹUTR are highly associated with half-life changes. a Random forest classifier for prediction of mRNA sequence features relevant for half-life fold-change in human NEURTT and in cortical brain samples of the mouse Mecp2 y/-. Percent accuracy (Y-axis) of half-life fold-change predictions in human NEURTT (b) from…

Mapping of ncRNA expression across mouse organs We sequenced 771 tissue samples of the TMS cohort to map molecular shifts across the whole organism during healthy aging (Fig. 1a). The protocol enriches for small ncRNA, especially mature miRNAs. Even though full-length reads cover only small ncRNAs (miRNAs or piwi-interacting RNAs…

A total of 47 SARS-CoV-2 positive samples with real-time PCR Ct values ranging from 14 to 30 were sequenced with three different protocols for SARS-CoV-2 sequencing, involving enrichment by bait hybridization or tiled amplicons. A schematic overview including library preparation, target enrichment, and sequencing is shown in Fig. 1. All 47…

1 Introduction Abiotic stresses such as drought, heat, cold, salt, pesticides, metals and metalloids deteriorate agro-ecological conditions and hence, negatively impact upon agricultural production. The abiotic stress impairs with regular growth and development of plant plants, thus limiting their yield and quality of produce (Boyer, 1982; Pandey et al., 2017). Plants…

Review . 2023 Mar 31;11:1146062. doi: 10.3389/fcell.2023.1146062. eCollection 2023. Affiliations Expand Affiliation 1 Group of Brain Development and Disease, Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. Item in Clipboard Review Vanessa Hall et al. Front Cell Dev Biol. 2023. Show details…

Background Cell-free DNA (cfDNA) refers to fragments of DNA released into the circulation by endothelial cells and white blood cells by active release or passively through apoptosis and necrosis.1,2 The fraction of cfDNA that originates from tumor is known as circulating tumor DNA (ctDNA). In 2016 the first commercial ctDNA…

Chimeric antigen receptor (CAR) T-cell therapies have demonstrated significant efficacy in patients with relapsed/refractory B-cell malignancies following multiple lines of therapy, with various CAR T cell products now U.S. Food and Drug Administration (FDA) approved for the treatment of B-cell acute lymphoblastic leukemia (B-ALL), diffuse large B-cell lymphoma (DLBCL), mantle-cell…

Knockdown of LFPRLR reduces splenic B-cell subsets in SLE-prone mice To investigate whether the PRL-LFPRLR axis raises the risk of initiation of B-cell malignancies, we compared SLE-prone MRL-lpr mice treated with either control SMO or LFPRLR SMO. Among SLE-prone models, we chose MRL-lpr mice because they accumulate genetic lesions indicative…

1 Introduction Cancer is one of the world’s most serious threats to human health, with high morbidity and mortality rates, and according to the latest global data, 9.96 million patients will die from cancer in 2020 (1). Cancer is a genetic abnormal disease triggered by a long-term combination of multiple…

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Keywords single guide RNA,sgRNA,RNA,ribonucleic acid,nucleic acid,guide RNA,cas9,gRNA,crRNA,crisprRNA,trans-activating RNA,tracrrna Read more here: Source link

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Use this icon in BioRender along with 1000s of others to make your next science figure in minutes SIGN UP FREE [{“image”:”res.cloudinary.com/dlcjuc3ej/image/upload/v1561473348/ebbn9qyuttq9yjor15pm.svg#/keystone/api/icons/5d12314684fc6c04006a9878/sequencer-illumina-miseq.svg”,”waterMarkImage”:”icons.biorender.com/w550xh620/5d12314684fc6c04006a9878/sequencer-illumina-miseq.png”},{“image”:”res.cloudinary.com/dlcjuc3ej/image/upload/v1561473348/xhihfjtej5g1stsxuskc.svg#/keystone/api/icons/5d12314684fc6c04006a9877/sequencer-illumina-miseq.svg”,”waterMarkImage”:”icons.biorender.com/w550xh620/5d12314684fc6c04006a9877/sequencer-illumina-miseq.png”},{“image”:”res.cloudinary.com/dlcjuc3ej/image/upload/v1573575912/a12usyeepidrrrsqosfd.svg#/keystone/api/icons/5dcadcea775675000410407c/sequencer-illumina-miseq.svg”,”waterMarkImage”:”icons.biorender.com/w550xh620/5dcadcea775675000410407c/sequencer-illumina-miseq.png”}] Keywords lab,lab equipment,equipment,technology,sequencer,sequencing,illumina,next-generation sequencing platform,sequencing platform,NGS,NextSeq,iSeq,DNA sequencer,RNA sequencer,RNA seq,DNA seq Read more here: Source link

A tool for genome editing: CRISPR-Cas9 We had the honor of collaborating with the Doudna lab to create this CRISPR-Cas9 infographic to celebrate their 2020 Nobel Prize in Chemistry. CRISPR-Cas9 is a tool for genome editing discovered by Dr. Jennifer Doudna and Dr. Emmanuelle Charpentier, who share the 2020 Nobel…

doi: 10.3389/fgene.2022.917993. eCollection 2022. Krinio Giannikou  1   2 , Katie R Martin  3 , Ahmad G Abdel-Azim  1 , Kaila J Pamir  4 , Thomas R Hougard  4 , Shefali Bagwe  4 , Yan Tang  4 , Jeffrey P MacKeigan  3 , David J Kwiatkowski  1 , Elizabeth P Henske  4 , Hilaire C Lam  4 Affiliations Expand Affiliations…

Nucleolar dominance: 90 years ago to present A nucleolus (see Glossary) is formed in the vicinity of the chromosomal locus whose tandemly repeated 35-48S rDNA is transcribed (Figure 1) [ 1. Nucleolar dominance: uniparental gene silencing on a multi-megabase scale in genetic hybrids. , 2. Nucleolar dominance: a ‘David and…

The human stories behind rare rheumatic diseases illustrate how genetic information can benefit patients. In 1988, a team of physicians met a patient with a unique disease of multiple organs, including the kidneys, liver, brain and eyes7. No immunosuppressive therapy was effective, and the patient and several of his family…

Ethics statement All fresh peripheral blood samples were obtained after approval of protocols by the Weizmann Institutional Review Board (IRB application 92-1) and following informed consent from the donors. The study using BAL fluid samples was approved by the Hadassah Medical Organization research ethics committee in accordance with the Declaration…

Introduction Patients with alveolar bone cleft need alveolar bone grafting to restore a normal maxilla structure, and the grafting of autogenous bone like iliac bone is still the most common choice (1, 2). However, bone loss after the surgery happens a lot (3). Clinical studies demonstrated that the operation age…

Sustained skeletal muscle mTORC1 activity upregulates the ubiquitin proteasome system To understand the molecular mechanisms causing atrophy in muscles with high mTORC1 activation, we first analyzed the transcriptome of the fast-type extensor digitorum longus (EDL) muscle in 3-month-old TSCmKO mice6. A short-term (3 day) treatment with rapamycin was used to…

Genomic features of two Anastatus wasps, A. japonicus and A. fulloi We employed PacBio high-fidelity (HiFi) long-read sequencing and Illumina short-read sequencing technologies to generate high-quality contigs for two Anastatus wasps, A. japonicus and A. fulloi (Supplementary Tables 1 and 2). These contigs were further scaffolded using Hi-C libraries to…

Joseph E Maakaron, assistant professor of medicine, Marie Hu, assistant professor of medicine, Najla El Jurdi, assistant professor of medicine University of Minnesota Twin Cities, Minneapolis, United States Correspondence to: maaka001{at}umn.edu Abstract Chimeric antigen receptor T cells have revolutionized the treatment of hematological malignancies during the past five years, boasting…

Mice A laboratory strain B6 was purchased from CLEA Japan Inc. (Tokyo, Japan), while nine wild strains (BFM/2, BLG2, CHD, HMI, KJR, MSM, NJL, PGN2, and SWN) were bred at NIG (Shizuoka, Japan) (Table 1). All mice were maintained at the animal facility in NIG, under specific-pathogen-free conditions and controlled…

Article Highlight | 15-Jun-2022 Researchers leverage viruses identified from worldwide environmental samples to expand knowledge of viral taxa and their role in tree microbiomes DOE/US Department of Energy image: Analysis of metatranscriptome samples with novel viral taxa in Populus. (Top) Graphical abstract of the pipeline. (Bottom) Percentage read classification with novel…

The clustered regularly interspaced short palindromic repeats-Cas (CRISPR-Cas) defensive system is an acquired mechanism in prokaryotes which is analogous to RNAi in eukaryotes [1]. The CRISPR machinery is a complex immune strategy that is continually evolving in the bacterial genome to accommodate the rapid changes in the nucleic acids of…

Circulating cell-free DNA (cfDNA) is fragmented extracellular DNA, which is released from apoptotic and necrotic cells in small fragments of <200 bp [1]. cfDNA is typically isolated from the blood stream; however, it is also possible to detect cfDNA in other biological fluids such as urine or cerebrospinal fluid [2, 3,…

Experimental workflow and vector designs One way to validate the all-in-one reporter system is through transient or stable transfection of mammalian cells. Within this framework, the schematics in Fig. 1 illustrate the overall workflow of this study and feature the characteristics of promoters. To summarize, the study involves the engineering and…

doi: 10.3389/fnmol.2022.852243. eCollection 2022. Lauren E Fritsch  1 , Jing Ju  2 , Erwin Kristobal Gudenschwager Basso  3 , Eman Soliman  3 , Swagatika Paul  4 , Jiang Chen  2 , Alexandra M Kaloss  3 , Elizabeth A Kowalski  3 , Taylor C Tuhy  5 , Rachana Deven Somaiya  1 , Xia Wang  3 , Irving Coy Allen  3 , Michelle H…

In nature, microbes have evolved different systems to sense external stimuli. Synthetic biology approaches (1) repurpose these systems as biosensors to specifically and sensitively detect various targets of interest. Although various highly sensitive and specific laboratory-based analytical methods (including high-performance liquid chromatography and mass spectrometry) can detect small-molecule targets, they…

By Sally Temple The Nobel Prize–winning discovery that mature, specialized cells can be reprogrammed to lend them pluripotency (that is, the ability to develop into all tissues of the body) unleashed vast potential in the fields of disease modeling, drug screening, regenerative medicine, and wound healing. The reprogrammed cells are…

Abstract Background A barrier to HIV-1 cure rests in the persistence of proviral DNA in infected CD4+ leukocytes. The high HIV-1 mutation rate leads to viral diversity, immune evasion, and consequent antiretroviral drug resistance. While CRISPR-spCas9 can eliminate latent proviral DNA, its efficacy is limited by HIV strain diversity and…

Study of RUNX2 locus in modern and ancient humans The sequences of the RUNX2 locus (Chr6:45,318,000–45,670,000 hg38, for a total of 352.000 bases) have been aligned in AMH and ancient species’ (Neandertal and Denisovan) genomes to map the changes occurred during recent evolution. We have identified 459 and 470 changes…

Introduction The overarching definition of precision pain medicine is that diagnosis and treatment can be customized to an individual’s specific risk profile.1,2 At its most basic, the ideology is based on using all available patient-level data to target therapies for that individual with regard to prediction, prevention, diagnosis, and treatment…

free online tools to build scientific figures/tables for journal/thesis 0 Dear All, What are some free and good online tools/websites to prepare scientific illustrations for publications/thesis and presentations ?I have heard of BioRender and google gives quite an extensive list. Thank you illustrations scientific figures • 31 views • link…