Tag: CAS

With CRISPR gene scissors in opposition to HIV: First try is underway within the USA

With CRISPR gene scissors in opposition to HIV: First try is underway within the USA On December 1st, World AIDS Day, information from the USA presents hope for these affected. In July 2022, the Californian biotech firm Excision BioTherapeutics dared to make use of a brand new remedy idea with…

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dharmacon sirna design

Please feel free to visit our Privacy Policy and Cookie Policy with regards to processing your data. Article Customer Support Sequence characteristics of functional siRNAs. Human and mouse genes are each targeted with multiple shRNA constructs that have been cloned into the pGIPZ lentiviral vector. GRCF Management Team. In the…

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A CRISPR endonuclease gene drive reveals distinct mechanisms of inheritance bias

CRISPR/Cas gene drives can bias transgene inheritance through different mechanisms. Homing drives are designed to replace a wild-type allele with a copy of a drive element on the homologous chromosome. In Aedes aegypti, the sex-determining locus is closely linked to the white gene, which was previously used as a target…

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Unexpected absence of ribosomal protein genes from metagenome-assembled genomes

Hug LA, Baker BJ, Anantharaman K, Brown CT, Probst AJ, Castelle CJ, et al. A new view of the tree of life. Nat Microbiol. 2016;1:16048. Article  PubMed  Google Scholar  Castelle CJ, Wrighton KC, Thomas BC, Hug LA, Brown CT, Wilkins MJ, et al. Genomic expansion of domain archaea highlights roles…

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Versatile and efficient genome editing with Neisseria cinerea Cas9

PAM identification of NcCas9 by PAM-DOSE The type II-C Cas9 orthologue NcCas9 is of small size (1082 aa) and is closely related to conventional Neisseria meningitidis Cas9 (NmeCas9)11 (Fig. 1a). The NcCas9 is 94% identical to Nme1Cas9, and the divergences lie mainly in the C-terminal PAM-interacting domain (PID) (Fig. 1a and S1)….

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Ancient origin and constrained evolution of the division and cell wall gene cluster in Bacteria

Miyakawa, T., Matsuzawa, H., Matsuhashi, M. & Sugino, Y. Cell wall peptidoglycan mutants of Escherichia coli K-12: existence of two clusters of genes, mra and mrb, for cell wall peptidoglycan biosynthesis. J. Bacteriol. 112, 950–958 (1972). Article  CAS  PubMed  PubMed Central  Google Scholar  Ayala, J. A., Garrido, T., De Pedro,…

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CRISPR/Cas editing tools

Sponsored Content by GenScriptNov 16 2022Reviewed by Maria Osipova In a new application for CRISPR/Cas editing tools, researchers at Harvard Medical School, Boston, Massachusetts, have created a CRISPR/Cas9 ribonucleoprotein-based peptide display approach. This technique is hoped to simplify the peptide library analysis workflow by using a new technique called peptide immobilization…

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New study finds that CRISPR/Cas9 leads to unexpected genomic changes

Credit: Unsplash/CC0 Public Domain The widely used gene scissor (CRISPR/Cas) can modify the genetic content in cells to study the molecular roles of genes and has gained great clinical relevance in gene therapy to treat genetic diseases. A new study performed by Claudia Kutter’s research group at the Department of…

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Multiplexed base editing through Cas12a variant-mediated cytosine and adenine base editors

Robust C-to-T conversion by Cas12a variant-mediated CBEs for non-canonical PAMs We initially attempted to screen for optimal cytosine deaminases that confer powerful C-to-T editing for Cas12a-mediated CBEs. Seven dCas12a-CBEs fused with individual cytosine deaminases1,2,16,20,21,22,24,25,26 were compared in HEK293T cells and porcine embryos at sites with TTTV PAM sequences. The dCas12a-A3A…

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How is RNA used in CRISPR and mRNA vaccines?

Why is RNA important in immunity? All organisms, from single-celled bacteria to blue whales, have a genome that’s made of deoxyribonucleic acid, or DNA. When it comes to viruses, many use single-stranded ribonucleic acid (RNA). However, certain species (such as rotavirus, which causes gastroenteritis) have a double-stranded RNA genome. The…

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Butterfly wing pattern ground plan is manipulated by non-coding regulatory DNA, study says

Butterfly wing patterns have a basic plan to them, which is manipulated by non-coding regulatory DNA to create the diversity of wings seen in different species, according to new research. The study, “Deep cis-regulatory homology of the butterfly wing pattern ground plan,” published as the cover story in the Oct….

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Health System Launches CRISPR-Based Biopharma

Credit: Design Cells/Getty Images Non-profit health system ChristianaCare, with hospitals and health facilities in Delaware, Maryland, Pennsylvania, New Jersey, announced today that it has spun out CorriXR Therapeutics, a biotechnology company that will leverage CRISPR gene editing technology to develop oncology therapeutics, with an initial focus on squamous cell carcinoma…

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Precise DNA cleavage using new CRISPR-Cas approach

A team led by investigators at Massachusetts General Hospital (MGH) has overcome a major constraint for cutting and editing DNA by CRISPR-Cas enzymes and other technologies. The recent innovation, which is published in Nature Biotechnology, will simplify and expedite molecular cloning approaches and expand their utility. CRISPR-Cas editing has transformed…

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Optimization of sgRNA expression strategy to generate multiplex gene-edited pigs

[1] Fan ZY, Liu ZG, Xu K, Wu TW, Ruan JX, Zheng XM, et al. 2022. Long-term, multidomain analyses to identify the breed and allelic effects in MSTN-edited pigs to overcome lameness and sustainably improve nutritional meat production. Science China Life Sciences, 65(2): 362−375. doi:…

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CRISPR-SpRYgests Enable Precise Cleavage of DNA Bases In Vitro

NEW YORK — A CRISPR-Cas variant engineered to no longer need a protospacer adjacent motif (PAM) can be harnessed to make cuts at any DNA base in vitro, a new study reports. Its ability to make precise breaks anywhere could be applied to a number of DNA engineering applications. Researchers…

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Three Noteworthy Applications of CRISPR

Only 10 years ago, Nobel prize-winning work introduced CRISPR, or clustered regularly interspaced short palindromic repeats, as a new and powerful genome editing tool. Since then, many exciting applications of the CRISPR-Cas9 system have been developed and proposed, giving a taste of what is yet to come.   Progress has…

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Should reproductive technology be inevitable?

Alan Kadish, M.D., president of the Touro College and University System. Photograph by William Taufic. Recently, Alan Kadish, M.D., and John D. Loike, Ph.D., co-authored an article in The Scientist magazine about the medical and ethical limits of such reproductive technologies as cloning and parthenogenesis (the creation of an organism, almost…

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Cas4-Cas1 is a Protospacer Adjacent Motif-Processing factor mediating half-site spacer integration 5 during CRISPR adaptation

@article{271b49f1d0724db19d00b53dbf126380, title = “Cas4-Cas1 is a Protospacer Adjacent Motif-Processing factor mediating half-site spacer integration 5 during CRISPR adaptation”, abstract = “The immunization of bacteria and archaea against invading viruses via CRISPR adaptation is critically reliant on the efficient capture, accurate processing, and integration of CRISPR spacers into the host genome….

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Simultaneously induced mutations in eIF4E genes by CRISPR/Cas9 enhance PVY resistance in tobacco

Pasin, F., Daròs, J.-A. & Tzanetakis, I. E. Proteome expansion in the Potyviridae evolutionary radiation. FEMS Microbiol. Rev. 46, 011. doi.org/10.1093/femsre/fuac011 (2022). Article  Google Scholar  Karasev, A. V. & Gray, S. M. Continuous and emerging challenges of Potato virus Y in potato. Annu. Rev. Phytopathol. 51, 571–586 (2013). CAS  PubMed …

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“Increasing Production of Therapeutic mAbs in CHO Cells through Genetic” by Charles Barentine

Abstract Between 2014 and 2018, the global market for therapeutic monoclonal antibodies (mAbs) rose from $60 billion to $115.2 billion with a projected value of $300 billion by 2025. These molecules are used to effectively treat some of the most challenging illnesses from auto-immune diseases to cancer. While mAbs are…

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Multidrug resistance microbial therapy | VMRR

Introduction Antimicrobials are the most important and useful therapeutic discovery in the history of medicine. It allows living beings to survive the microbial disease, enhances invasive surgical procedures, ensures animal health, and protects the food chain since 1928 after the discovery of penicillin. However, the indiscriminate use of antibiotics and…

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Solved Which of the following are true about CRISPR

Transcribed image text: Which of the following are true about CRISPR delivery? Non-viral methods result in long term expression of Cas 9 which increases the likelihood of off-target editing. Viruses have naturally evolved tropisms to infect certain cell types, therefore we can harness these viral vectors to deliver Cas 9…

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New dual-plasmid editing system for DNA-based information rewriting in vivo

DNA-based information is a new interdisciplinary field linking information technology and biotechnology. The field hopes to meet the enormous need for long-term data storage by using DNA as an information storage medium. Despite DNA’s promise of strong stability, high storage density and low maintenance cost, however, researchers face problems accurately…

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Intellia: Slim Hopes Of Near-Term Commercial Revenue, Bearish

liulolo/iStock via Getty Images Investment Thesis If you had invested in the CRISPR / Cas9 gene editing pioneer Intellia Therapeutics (NASDAQ:NTLA) five years ago, you would likely be well satisfied with your return to date – a ~285% return on your investment – although you may also feel that it…

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CAS9 Technology Market, Globally Trend MRFR Largest Share Trending Technology, Recent Developments And Future According To Emerge Research Report Forecast By 2027

Market Scenario: The commercial interest and rise in the application will bring new growth opportunities to boost the CAS9 technology market in the upcoming years. The market is estimated to soar at a compound annual growth rate of 11.30% by 2023.  The science field is developing at a rapid speed;…

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CRISPR ‘cousin’ put to the test in landmark heart-disease trial

Base editing could edit genomes like CRISPR, but with less potential for unwanted effects.Credit: Martin Steinthaler/Getty It’s test time for CRISPR’s cousin. A clinical trial that recently treated its first participant will test whether base-editing — a genome-editing method related to the CRISPR–Cas9 system — can safely be used to…

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Amount of Cas9 protein introduced into mouse embryos via electroporation affects the genome-editing rate

doi: 10.1262/jrd.2022-067. Online ahead of print. Affiliations Expand Affiliations 1 Division of Science and Engineering, Graduate School of Arts and Science, Iwate University, Iwate 020-8551, Japan. 2 Integrated DNA Technologies, Inc., Coralville, IA 52241, U.S.A. 3 Department of Chemistry and Biological Sciences, Faculty of Science and Engineering, Iwate University, Iwate 020-8551,…

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CRISPR Gene Detection and Diagnostic Industry 2022 Market Size, Share and Growth, Global Segments Analysis and Dynamic Research Report 2029

The Wide Ranging CRISPR Gene Detection and Diagnostic Business Report encompasses a thorough study of current situation of the global market along with several market dynamics. The market research report is a demonstrated source of information which offers a telescopic view of the current market trends, size, share, growth, demand,…

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2 Common SCD Mouse Models Not Suited for Gene Editing Studies | Models Limit Work Into Fetal Hemoglobin for Sickle Cell

Two mouse models commonly used to study sickle cell disease (SCD) are not suitable for testing certain gene editing treatment approaches, including those for fetal hemoglobin production, because of their specific genetic makeup, a study reported. “This work provides a reminder for scientists to carefully consider the genetics of the…

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The evolutionary origin of host association in the Rickettsiales

Salje, J. Cells within cells: Rickettsiales and the obligate intracellular bacterial lifestyle. Nat. Rev. Microbiol. 19, 375–390 (2021). CAS  PubMed  Article  Google Scholar  Wang, S. & Luo, H. Dating Alphaproteobacteria evolution with eukaryotic fossils. Nat. Commun. 12, 3324 (2021). CAS  PubMed  PubMed Central  Article  Google Scholar  Strassert, J. F. H.,…

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A fast, highly sensitive, point-of-care RT-LAMP and CRISPR/Cas12a assay for the detection of SARS-CoV-2 & More Breaking News

  In a latest research posted to the Research Square* preprint server, researchers used isothermal amplification with clustered often interspaced brief palindromic repeats (CRISPR)-Cas12a to detect extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2).  Study: Rapid, multiplex detection of SARS-CoV-2 utilizing isothermal amplification coupled with CRISPR-Cas12a. Image Credit: creativeneko/Shutterstock Background To…

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Three families of Asgard archaeal viruses identified in metagenome-assembled genomes

Liu, Y. et al. Expanded diversity of Asgard archaea and their relationships with eukaryotes. Nature 593, 553–557 (2021). CAS  PubMed  Google Scholar  Dombrowski, N., Teske, A. P. & Baker, B. J. Expansive microbial metabolic versatility and biodiversity in dynamic Guaymas Basin hydrothermal sediments. Nat. Commun. 9, 4999 (2018). PubMed  PubMed…

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SARS-Cov2 and Crispr-Cas Gene Assignment – Cracking the Code for SARS-Cov2 and the Discovery of

Cracking the Code for SARS-Cov2 and the Discovery of CRISPR-Cas Gene Editing Mechanism SARS-Cov2 1.) Describe the morphological structure of SARS-Cov2 virus. – According to studies through the use of Electron microscopy, the structure of SARS-CoV-2 is speculated to be similar to that of SARS-CoV with a virion size ranging…

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CRISPR-Cas technology a new era in genomic engineering

Review doi: 10.1016/j.btre.2022.e00731. eCollection 2022 Jun. Affiliations Expand Affiliation 1 Department of Agriculture and Natural Resources, College of Agriculture, Science, and Technology, Delaware State University, Dover, DE 19901, United States of America. Free PMC article Item in Clipboard Review Ali Parsaeimehr et al. Biotechnol Rep (Amst). 2022. Free PMC article Show…

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RCSB PDB – 7SBA: Structure of type I-D Cascade bound to a dsDNA target

CRISPR-Cas systems are adaptive immune systems that protect prokaryotes from foreign nucleic acids, such as bacteriophages. Two of the most prevalent CRISPR-Cas systems include type I and type III. Interestingly, the type I-D interference proteins contain characteristic features of both type I and type III systems … CRISPR-Cas systems are…

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Bio-informatic analysis of CRISPR protospacer adjacent motifs (PAMs) in T4 genome | BMC Genomic Data

The clustered regularly interspaced short palindromic repeats-Cas (CRISPR-Cas) defensive system is an acquired mechanism in prokaryotes which is analogous to RNAi in eukaryotes [1]. The CRISPR machinery is a complex immune strategy that is continually evolving in the bacterial genome to accommodate the rapid changes in the nucleic acids of…

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Frontiers | A Mutated Nme1Cas9 Is a Functional Alternative RNase to Both LwaCas13a and RfxCas13d in the Yeast S. cerevisiae

Introduction The clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated (Cas) protein systems naturally exist in prokaryotes. They are RNA-mediated defense mechanisms to protect bacteria and archaea from invading nucleic acids (Barrangou et al., 2007). CRISPR–Cas systems are classified into two classes (1 and 2). Class 2 CRISPR–Cas require a single…

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Convenience-sized RNA editing | MIT News

Last year, researchers at MIT’s McGovern Institute for Brain Research discovered and characterized Cas7-11, the first CRISPR enzyme capable of making precise, guided cuts to strands of RNA without harming cells in the process. Now, working with collaborators at the University of Tokyo, the same team has revealed that Cas7-11…

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Study Reveals High Turnover and Rescue Effect of Scaffold Protein in Response to Heavy-ion Radiation—-Chinese Academy of Sciences

Researchers from the Microbeam Technology and Applications Group at the Institute of Modern Physics (IMP) of the Chinese Academy of Sciences (CAS) have discovered the high turnover and rescue effect of an important scaffold protein in response to heavy charged particle radiation. The results were published in Biophysical Journal.   DNA damage response and repair play a decisive role in cell…

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In vivo hypermutation and continuous evolution

Arnold, F. H. Design by directed evolution. Acc. Chem. Res. 31, 125–131 (1998). Google Scholar  Packer, M. S. & Liu, D. R. Methods for the directed evolution of proteins. Nat. Rev. Genet. 16, 379–394 (2015). Google Scholar  Drake, J. W., Charlesworth, B., Charlesworth, D. & Crow, J. F. Rates of…

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Molecular mechanism of ZDHHC18-mediated palmitoylation of cGAS in innate immunity inhibition

Detection of double-stranded DNA by cGAS triggers innate immune responses. ZDHHC18-mediated palmitoylation of cGAS sheds light on a novel posttranslational modification that leads to the fine-tuning of cGAS-mediated innate immune responses. Credit: The EMBO Journal (2022). DOI: 10.15252/embj.2021109272 Cyclic GMP-AMP synthase (cGAS), a double-stranded DNA (dsDNA) sensing protein, plays an…

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WGS Facilitates Gene Editing System Upgrade

Researchers at the Korean Institute of Life Sciences and Technology engineered an efficient, miniaturized CRISPR-Cas gene-editing system that may be more easily packed into vectors for clinical applications. Their system employs the Cas variant Cas12f1 with a guide RNA (gRNA) remodeled to mitigate off-target effects, a design that could potentially…

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Frontiers | Divergence With Gene Flow and Contrasting Population Size Blur the Species Boundary in Cycas Sect. Asiorientales, as Inferred From Morphology and RAD-Seq Data

Introduction Incipient species are critical for evolutionary biologists to study speciation, but they also challenge taxonomy due to gene flow or ancestral polymorphism. The former and contrasting population size lead to larger intraspecific than interspecific variations, a phenomenon called the species-definition anomaly zone (Jiao and Yang, 2021). The latter results…

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Recent developments in miRNA based recombinant protein expression in CHO

Aguiar TQ, Santos SB, Martins IM, Domingues L, Oliveira C (2019) Production and bioengineering of recombinant pharmaceuticals. Proteins: sustainable source, processing and applications. Elsevier, Amsterdam, pp 259–293 Chapter  Google Scholar  Amadi IM, Agrawal V, Christianson T, Bardliving C, Shamlou P, LeBowitz JH (2020) Inhibition of endogenous miR-23a/miR-377 in CHO cells…

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Plant Breeding and CRISPR Plants Market Trends 2022, Global Industry Share, Size, Global Industry Analysis, Key Market Growth Analysis, Segments, Emerging Technologies, Opportunity and Forecast 2027

The “Plant Breeding and CRISPR Plants Market” research report 2021-2027 offering the complete outline of market size, share and growth opportunities, industry segmentation by product type, applications and geographical representation. The report highlights product development along with technological upgrades that can boost the market growth. The Plant Breeding and CRISPR…

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Biogenesis, biology and characterization of circular RNAs

Biogenesis, biology and characterization of circular RNAs Kristensen LS et al. The biogenesis, biology and characterization of circular RNAs. Nat Rev Genet. (2019) Summary text Biogenesis and properties of circRNAs Biogenesis of cirRNA Characteristics of circRNAs Discover and analyze circRNAs circRNA genome-wide analysis CircRNA site-specific analysis circRNA visualization Biological functions…

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Multiplexed genome regulation in vivo with hyper-efficient Cas12a

. 2022 Apr;24(4):590-600. doi: 10.1038/s41556-022-00870-7. Epub 2022 Apr 12. Lucie Y Guo #  1   2 , Jing Bian #  3 , Alexander E Davis  4 , Pingting Liu  4 , Hannah R Kempton  3 , Xiaowei Zhang  3 , Augustine Chemparathy  3 , Baokun Gu  3 , Xueqiu Lin  3 , Draven A Rane  3 , Xiaoshu Xu  3 , Ryan M…

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Genome Editing of Human Primary T Cells With Lipid Nanoparticles

By Reka Geczy, PhD, Aruna Balgi, Stella Park, Rita Zhao, Ethan Watt, Maggie Wong, Cooper Webb, Nikita Jain, PhD, Angela Zhang, PhD, Anitha Thomas, PhD, Samuel Clarke, PhD The expression of the chimeric antigen receptor (CAR) on T cells turns a patient’s cells into cell-based cancer therapies and has revolutionized…

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PhD in functional genomics, antifungal discovery, synthetic biology (1.0 FTE)

Category Research / Academic Location Groningen The group of Dr. Sonja Billerbeck has an open position for a 4-year PhD project. In the Billerbeck lab, we use a combination of synthetic biology, genome engineering, protein engineering, and environmental microbiology to access, understand and engineer the functional diversity of nature’s yeast-based…

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Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults

The goal of this protocol is to detect phenolic metabolites in plasma using a semi-targeted chromatography-mass spectrometry method. The goal of this protocol is to detect phenolic metabolites in plasma by a semi-targeted UPLC-MS/MS method. Plasma proteins were precipitated with ethanol and samples concentrated, and re-suspended in the mobile phase,…

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CRISPR-VAE: A Method for Explaining CRISPR/Cas12a Predictions, and an Efficiency-aware gRNA Sequence Generator

Abstract Deep learning has shown great promise in the prediction of the gRNA efficiency, which helps optimize the engineered gRNAs, and thus has greatly improved the usage of CRISPR-Cas systems in genome editing. However, the black box prediction of deep learning methods does not provide adequate explanation to the factors…

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Label-Free Resonance Rayleigh Scattering Amplification for Lipopolysaccharide Detection and Logical Circuit by CRISPR/Cas12a-Driven Guanine Nanowire Assisted Non-Cross-Linking Hybridization Chain Reaction,Analytical Chemistry

Although the CRISPR/Cas system has pioneered a new generation of analytical techniques, there remain many challenges in developing a label-free, accurate, and reliable CRISPR/Cas-based assay for reporting the levels of low abundance biomolecules in complex biological samples. Here, we reported a novel CRISPR-derived resonance Rayleigh scattering (RRS) amplification strategy and…

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CRISPRi for specific inhibition of miRNA clusters and miRNAs with high sequence homology

Bartel, D. P. MicroRNAs: Target recognition and regulatory functions. Cell 136, 215–233 (2009). CAS  Article  Google Scholar  Bassett, A. R. et al. Understanding functional miRNA–target interactions in vivo by site-specific genome engineering. Nat. Commun. 5, 4640 (2014). ADS  CAS  Article  Google Scholar  Moore, M. J. et al. miRNA–target chimeras reveal…

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CASCADE: Naked eye-detection of SARS-CoV-2 using Cas13a and gold nanoparticles

This article was originally published here Anal Chim Acta. 2022 May 1;1205:339749. doi: 10.1016/j.aca.2022.339749. Epub 2022 Mar 22. ABSTRACT The COVID-19 pandemic has brought to light the need for fast and sensitive detection methods to prevent the spread of pathogens. The scientific community is making a great effort to design…

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RCSB PDB – 7V94: Cryo-EM structure of the Cas12c2-sgRNA-target DNA ternary complex

RNA-guided CRISPR-Cas nucleases are widely used as versatile genome-engineering tools. Recent studies identified functionally divergent type V Cas12 family enzymes. Among them, Cas12c2 binds a CRISPR RNA (crRNA) and a trans-activating crRNA (tracrRNA) and recognizes double-stranded DNA targets with a short TN PAM … RNA-guided CRISPR-Cas nucleases are widely used…

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A general RPA-CRISPR/Cas12a sensing platform for Brucella spp. detection in blood and milk samples

Available online 9 April 2022, 131864 doi.org/10.1016/j.snb.2022.131864Get rights and content Highlights • We firstly introduce a general RPA-CRISPR/Cas12a sensing platform into fluorescent and electrochemical dual-signal output. • We firstly established an isothermal, rapid, specific and ultrasensitive RPA-CRISPR/Cas12a sensing platform for Brucella spp. detection. • This platform enabled rapid and accurate…

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Activation of STING by targeting a pocket in the transmembrane domain

Zhang, X., Bai, XC & Chen, ZJ Structures and mechanisms of the cGAS-STING innate immunity pathway. Immunity 5343–53 (2020). CAS PubMed Google Scholar Article Ishikawa, H. & Barber, GN STING is an endoplasmic reticulum adapter that facilitates innate immune signaling. Nature 455674–678 (2008). ADS CAS PubMed PubMed Central Article Google…

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Gene Editing Methods for Gene Therapy

Scientists at Creative Biolabs provide TALENs and CRISPR/Cas gene editing services to clients all over the world enabling investigators to disrupt, add, and correct any gene in a diverse range of cell types and organisms…. More Scientists at Creative Biolabs provide TALENs and CRISPR/Cas gene editing services to clients all…

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Pacific Biosciences, Corteva Agriscience Collaborate on High-Throughput Sequencing

NEW YORK – Pacific Biosciences said on Monday that it is collaborating with Corteva Agriscience to develop custom workflows for plant, pest, and microbial sequencing. Under the terms of the project, the partners will establish high-throughput workflows for DNA extraction and library preparation to enable sequencing of thousands of samples….

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What is genetic engineering and how can it benefit healthcare?

Have you heard of purple tomatoes? Or golden rice? If you haven’t, you might be surprised to know that these have actually been created by scientists via genetic engineering. These are genetically modified foods, with purple tomatoes containing 10 times more antioxidants than a normal red tomato. On the other…

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Using CRISPR/Cas to develop biosafety materials

The diagnosis and treatment of viral infections, especially those that cause large-scale outbreaks, is urgently needed, as the lack of these measures allows epidemics to progress. A new Biosafety and Health study discusses the use of the highly efficient clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) technology to…

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Sigma-Aldrich Files Opposition to CVC Substantive Preliminary Motion No. 1 to be Accorded Priority Benefit | McDonnell Boehnen Hulbert & Berghoff LLP

On February 18th, Sigma-Aldrich filed its Opposition to Junior Party’s (the University of California, Berkeley; the University of Vienna; and Emmanuelle Charpentier; collectively, “CVC”) Substantive Preliminary Motion No. 1 in Interference No. 106,132, asking the Patent Trial and Appeal Board for benefit of priority to U.S. Provisional Application No. 61/652,086,…

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Cutting Edge Agriculture Gene Editing with Cas-CLOVER

Blog Agriculture Biotechnology Though CRISPR-Cas enabled targeted genome engineering across a vast array of organisms, the system features major disadvantages. Frequent off-target mutagenesis, licensing restrictions and non-ideal economic license terms have inhibited commercial crop-science product upscaling, and, in many cases, entirely disqualified CRISPR’s use by many commercial crop developers. Seeking…

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CRISPR/Cas12a-Enhanced Loop-Mediated Isothermal Amplification for the Visual Detection of Shigella flexneri

doi: 10.3389/fbioe.2022.845688. eCollection 2022. Affiliations Expand Affiliations 1 Provincial Key Laboratory for Transfusion-Transmitted Infectious Diseases, Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, China. 2 Clinical Laboratory Department, Yan’an Hospital Affiliated to Kunming Medical University, Kunming, China. 3 Department of Emergency, The Traditional…

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Cho Cell Line Development & Engineering: Impact On Biopharmaceutical Production: Essay Example, 2571 words

1. Introduction The approval of Chinese hamster ovary (CHO) cell cultures for the manufacturing of protein therapeutic products modernized conventional medicine. CHO cells remained the workhorse for the production of monoclonal antibodies (mAbs) for the last at least 20 years for different reasons. First, CHO cells are easily adapting and…

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Development of Cas12a-Based Cell-Free Small-Molecule Biosensors via Allosteric Regulation of CRISPR Array Expression

In nature, microbes have evolved different systems to sense external stimuli. Synthetic biology approaches (1) repurpose these systems as biosensors to specifically and sensitively detect various targets of interest. Although various highly sensitive and specific laboratory-based analytical methods (including high-performance liquid chromatography and mass spectrometry) can detect small-molecule targets, they…

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CRISPR/Cas12a-based technology: A powerful tool for biosensing in food safety

Background: In the context of the current pandemic caused by the novel coronavirus, molecular detection is not limited to the clinical laboratory, but also faces the challenge of the complex and variable real-time detection fields. A series of novel coronavirus events were detected in the process of food cold chain…

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LAMP-CRISPR-Cas12a-lateral flow immunochromatographic strip | IDR

Introduction About 700,000 people die from “superbugs” globally every year. Antibiotic abuse is the culprit of extensive spread of superbugs. Currently, carbapenemase-producing organisms are among the most important pathogens of hospital infections. Especially the plasmid-mediated highly transmissible carbapenem-resistant Enterobacterales (CRE) has become an important public health issue of global concern….

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Hot Plasmids – February 2022

Every few months we highlight a subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they’ll make it easier for you to find and use the plasmids you need. If you’d ever…

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The comparisonof the function of crRNA (cr-ribonucleic acid) and tracrRNA (trans-activating crRNA) in the CRISPR-Cas(clustered regularly interspaced short palindromic repeats-CRISPR associated protein 9)system. Introduction: The CRISPR-Cas system is a crucial defense system against foreign invaders, like bacteriophage, transposons, and plasmids in many prokaryotes. There are five genes present on it: tracr, Cas1, Crispr, Cas2, Cas9 on the CRISPR. The tracrRNA and the crRNA have a guiding function in the expression phase of the defense mechanism.

Chapter 17, Problem 11CONQ To review: The comparisonof the function of crRNA (cr-ribonucleic acid) and tracrRNA (trans-activating crRNA) in the CRISPR-Cas(clustered regularly interspaced short palindromic repeats-CRISPR associated protein 9)system. Introduction: The CRISPR-Cas system is a crucial defense system against foreign invaders, like bacteriophage, transposons, and plasmids in many prokaryotes….

Continue Reading The comparisonof the function of crRNA (cr-ribonucleic acid) and tracrRNA (trans-activating crRNA) in the CRISPR-Cas(clustered regularly interspaced short palindromic repeats-CRISPR associated protein 9)system. Introduction: The CRISPR-Cas system is a crucial defense system against foreign invaders, like bacteriophage, transposons, and plasmids in many prokaryotes. There are five genes present on it: tracr, Cas1, Crispr, Cas2, Cas9 on the CRISPR. The tracrRNA and the crRNA have a guiding function in the expression phase of the defense mechanism.

Sigma-Aldrich Files Substantive Preliminary Motion No. 1 to Deny Broad Priority Benefit to Its Earliest-filed Provisional Application | McDonnell Boehnen Hulbert & Berghoff LLP

On December 3rd, Senior Party Sigma-Aldrich filed its Substantive Preliminary Motion No. 1 in Interference No. 106,133 (which names the Broad Institute, Harvard University, and MIT (collectively, Broad) as Junior Party), asking the Patent Trial and Appeal Board to deny Broad benefit of its U.S. Provisional Application No. 61/736,527, filed…

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CRISPR-Cas9 Gene Therapy for Duchenne Muscular Dystrophy

Ishino Y, Shinagawa H, Makino K, et al. Nucleotide sequence of the iap gene, responsible for alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product. J Bacteriol. 1987;169:5429–33. CAS  PubMed  PubMed Central  Google Scholar  Jansen R, van Embden JDA, Gaastra W, et al. Identification of genes…

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Novel CRISPR platform to decode the immune system

Date: 11th February 2022 The immune system is a critical biological network of processes that protects an organism from disease, and depends on the ability to distinguish self from non-self, a role driven by antigens.  In humans, T cells respond to antigen stimulation together with the production of cytokines however,…

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dCas9-VPR-mediated transcriptional activation of functionally equivalent genes for gene therapy

1. Dunbar, C. E. et al. Gene therapy comes of age. Science 359, eaan4672 (2018). PubMed Google Scholar  2. Wang, D., Tai, P. W. L. & Gao, G. Adeno-associated virus vector as a platform for gene therapy delivery. Nat. Rev. Drug Discov. 18, 358–378 (2019). CAS PubMed PubMed Central Google Scholar  3. Eid, A.,…

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Multi-organoid system to simulate human liver

image: Enlarged view of the co-culture system view more  Credit: Advanced Science Type 2 diabetes (T2DM) is a systematic multi-organ metabolic disease, which is characterized by dynamic interplay among different organs. Pancreatic islet-liver axis is closely associated with normal glucose regulation and homeostasis maintenance. The dysfunction of the interplay between these…

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Seven technologies to watch in 2022

The Telomere-to-Telomere Consortium is sequencing whole chromosomes.Credit: Adrian T. Sumner/SPL From gene editing to protein-structure determination to quantum computing, here are seven technologies that are likely to have an impact on science in the year ahead. Fully finished genomes Roughly one-tenth of the human genome remained uncharted when genomics researchers…

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Newly discovered protein essential for CRISPR/Cas3 genome editing

24 January 2022 A hidden component of the CRISPR/Cas3 genome editing system, which is essential for it to work, has been discovered by scientists. The CRISPR/Cas3 family of genome editing systems is similar to other CRISPR families but can uniquely make larger and more accurate changes to DNA (see BioNews…

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Multi-organoid system to simulate human liver-islet axis in normal and type 2 diabetes

Schematic of hiPSCs derived multi-organoid-on-chip system to model human liver-pancreatic islet axis in vitro. Credit: Tao Tingting Type 2 diabetes (T2DM) is a systematic multi-organ metabolic disease, which is characterized by dynamic interplay among different organs. Pancreatic islet-liver axis is closely associated with normal glucose regulation and homeostasis maintenance. The…

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Xenotransplantation: Argentina is about to produce genetically modified animals for human transplants

While the international medical community celebrates the first successful xenotransplantation, in Argentina two research teams from public universities are preparing to produce genetically modified animals so that their organs are suitable for human transplants and the first pigs of this type would be obtained at the end of this year…

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CRISPR-Cas12a ribonucleoprotein-mediated gene editing in the plant pathogenic fungus Magnaporthe oryzae

. 2021 Dec 24;3(1):101072. doi: 10.1016/j.xpro.2021.101072. eCollection 2022 Mar 18. Affiliations Expand Affiliation 1 Department of Plant Pathology, Kansas State University, Manhattan, KS, USA. Free PMC article Item in Clipboard Jun Huang et al. STAR Protoc. 2021. Free PMC article Show details Display options Display options Format AbstractPubMedPMID . 2021 Dec…

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Nutritionally charged lettuce developed with CRISPR/Cas gene editing tech – CNW News – Breaking News & Top Stories – Latest World, US & Local News

The concept of genetic modification can be described as polarizing. Some people believe that both traditional and modern plant breeding techniques are unsafe and unnatural. Others see the potential for greater yields and better nutrition. Yarin LIVNEH, a PhD student at Hebrew University in Israel, is part of the second…

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UMass Chan Medical School hiring Research Lab Tech I – Neurogenerative Disease (CRISPR-Cas gene editing) in Worcester, Massachusetts, United States

OverviewGENERAL SUMMARY OF POSITION: We are seeking a Research Technician to work collaboratively on CRISPR-Cas gene editing of the Huntington’s Disease (HD) gene. HD is a devasting neurodegenerative disease that currently has no effective treatment. Our goal is to develop gene editing methods to inactivate or correct the disease-causing version…

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CRISPR/Cas12a-based biosensors for ultrasensitive tobramycin detection with single- and double-stranded DNA activators

Dawei Li received his Bachelor’s degree (2003) and Master’s degree (2006) from Lanzhou University and PhD (2013) from Nanyang Technological University. At present he is an associate professor in college of biology and the environment at Nanjing Forestry University. His current research interests mainly focus on the design and synthesis…

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The CRISPR-Cas toolbox and gene editing technologies

The emergence of CRISPR-Cas systems has accelerated the development of gene editing technologies, which are widely used in the life sciences. To improve the performance of these systems, workers have engineered and developed a variety of CRISPR-Cas tools with a broader range of targets, higher efficiency and specificity, and greater…

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A year of ToBRFV

Although 2021 was the year of viruses for the entire world population, it was even more so for growers, who were confronted with the infamous Tomato Brown Rugose Fruit Virus (ToBRFV). It has been quite a ride – which is, unfortunately, not over just yet. In order to give you…

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CRISPR/Cas12a Powered DNA Framework-Supported Electrochemical Biosensing Platform for Ultrasensitive Nucleic Acid Analysis

Nucleic acid analysis using ultrasensitive and simple methods is critically important for the early-stage diagnosis and treatment of diseases. The CRISPR/Cas proteins, guided by a single-stranded RNA have shown incredible capability for sequence-specific targeting and detection. Herein, in order to improve and expand the application of CRISPR/Cas technology to the…

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Sigma-Aldrich Joins the CRISPR Interference Fray | McDonnell Boehnen Hulbert & Berghoff LLP

On June 21st,* the Patent Trial and Appeal Board declared two new interferences involving CRISPR technology.  The first, Interference No. 106,132, named Sigma-Aldrich as Senior Party and the University of California/Berkeley, the University of Vienna, and Emmanuelle Charpentier (collectively, “CVC”) as Junior Party, while the second, Interference No. 106,133 named…

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“Bushy plant growth is not an invention”

No Patents on Seeds! is filing an opposition against a Nunhems (BASF) patent on watermelons (EP2814316) derived from conventional breeding. The bushy plants were originally discovered in a garden. They argue that the genetic predisposition to bushy growth occurred randomly. Nunhems has, in this case, simply used well-known methods to…

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DEFENCE THERAPEUTICS ACCUMTM BOOSTS BY 9-FOLD THE DELIVERY EFFECTIVENESS OF THE CRISPR/CAS9 PROTEIN TO TARGET CELLS

DGAP-News: Defence Therapeutics Inc. / Key word(s): Miscellaneous 13.12.2021 / 08:30 The issuer is solely responsible for the content of this announcement. CSE: DTC FSE: DTC USOTC: DTCFF PRESS RELEASE DEFENCE THERAPEUTICS ACCUMTM BOOSTS BY 9-FOLD THE DELIVERY EFFECTIVENESS OF THE CRISPR/CAS9 PROTEIN TO TARGET CELLS Vancouver, BC, Canada, December…

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ADVANCED ELECTROPORATION TECHNIQUES in Biology and – EUR 104,90

Vendeur: bookandchopine ✉️ (6.022) 97.6%, Lieu où se trouve: ÉTOILE-SUR-RHÔNE, FR, Lieu de livraison: EUROPE, Numéro de l’objet: 255274816286 Advanced Electroporation Techniques in Biology and. BookAndChopine exerce son activité sur le web depuis plus de douze années maintenant. Les envois standard ne sont pas remboursés en cas de perte, c’est…

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HIRA complex presets transcriptional potential through coordinating depositions of the histone variants H3.3 and H2A.Z on the poised genes in mESCs

doi: 10.1093/nar/gkab1221. Online ahead of print. Yang Yang  1   2 , Liwei Zhang  3 , Chaoyang Xiong  2 , Jun Chen  2   4   5 , Li Wang  6 , Zengqi Wen  2   5 , Juan Yu  2 , Ping Chen  4 , Yanhui Xu  6 , Jingji Jin  1   7   8 , Yong Cai …

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Targeted mutagenesis in plants using Beet curly top virus for efficient delivery of CRISPR/Cas12a components

Available online 8 December 2021 doi.org/10.1016/j.nbt.2021.12.002Get rights and content Highlights • An efficient geminiviral vector was developed from Beet curly top virus with a wide host range. • The geminiviral vector delivered CRISPR/Cas components efficiently into plant cells. • A high frequency of both mutagenesis and complete HDR in plant…

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An assessment of pH-sensitive cationic lipid nanoparticles for drug delivery

A recent review in the Chemical and Pharmaceutical Bulletin looks at the molecular design of pH-sensitive cationic lipids and their applications for various tissues and cell types. The researchers also describe the application of lipid nanoparticles (LNPs) for delivering various macromolecules including small interfering ribonucleic acid (siRNA), antisense oligonucleotide, messenger…

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CRISPR Pioneers Doudna and Banfield Introduce Microbiome Community Editing

CRISPR systems have been developed, to date, to edit the genes of one type of cell at a time. Now, a new technique has been characterized that can add or modify genes within a community of many different species simultaneously, opening the door to the idea of “community editing.” The…

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BRAIN Biotech AG: Additional Genome Editing Nuclease: BRAIN-Metagenome-Cas 01 (BMC01)

DGAP-News: BRAIN Biotech AG / Key word(s): Miscellaneous/Patent08.12.2021 / 15:00 The issuer is solely responsible for the content of this announcement. Zwingenberg, Germany, December 8th, 2021 Additional Genome Editing Nuclease: BRAIN-Metagenome-Cas 01 (BMC01) BRAIN Biotech AG has successfully extended its proprietary genome editing toolbox with the addition of BRAIN-Metagenome-Cas 01…

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BRAIN Biotech AG: Additional Differentiated Genome-Editing Nuclease: BRAIN-Metagenome-Cas 01 (BMC01)

DGAP-Ad-hoc: BRAIN Biotech AG / Key word(s): Miscellaneous/PatentBRAIN Biotech AG: Additional Differentiated Genome-Editing Nuclease: BRAIN-Metagenome-Cas 01 (BMC01) 08-Dec-2021 / 14:53 CET/CESTDisclosure of an inside information acc. to Article 17 MAR of the Regulation (EU) No 596/2014, transmitted by DGAP – a…

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CRISPR/Cas9 gene editing boosts effectiveness

image: Ultrasound irradiation of a tumor causes a lipid nanoparticle (drawing shown above) to release harmful ROS and a CRISPR/Cas9 gene-editing system. view more  Credit: Credit: Adapted from ACS Central Science 2021, DOI: 10.1021/acscentsci.1c01143 Sonodynamic therapy uses ultrasound in combination with drugs to release harmful reactive oxygen species (ROS) at the…

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RNA-targeting enzyme expands the CRISPR toolkit

McGovern Fellows Jonathan Gootenberg (left) and Omar Abudayyeh in their lab. Credit: Caitlin Cunningham Researchers at MIT’s McGovern Institute for Brain Research have discovered a bacterial enzyme that they say could expand scientists’ CRISPR toolkit, making it easy to cut and edit RNA with the kind of precision that, until…

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CVC Files Opposition to ToolGen Substantive Motion No. 1 | McDonnell Boehnen Hulbert & Berghoff LLP

On July 15th, Junior Party the University of California/Berkeley, the University of Vienna, and Emmanuelle Charpentier (collectively, “CVC”) filed its Opposition to Senior Party ToolGen’s Substantive Motion No. 1 for benefit of priority to U.S. Provisional Application No. 61/837,481, filed June 20, 2013 (“P3” or “ToolGen P3”), or alternatively, International…

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Researchers Engineer a Mini CRISPR Genetic Editing System That Could Be Easier To Deliver Into Cells

CRISPR illustration. Credit: National Institutes of Health A compact and efficient CRISPR-Cas system, named CasMINI, could be broadly useful for cell-engineering and gene-therapy applications because it is easier to deliver into cells. The findings appear in a study that was published on September 3, 2021, in the journal Molecular Cell….

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