Tag: Cas12a

  In a latest research posted to the Research Square* preprint server, researchers used isothermal amplification with clustered often interspaced brief palindromic repeats (CRISPR)-Cas12a to detect extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2).  Study: Rapid, multiplex detection of SARS-CoV-2 utilizing isothermal amplification coupled with CRISPR-Cas12a. Image Credit: creativeneko/Shutterstock Background To…

BERKELEY, Calif., May 11, 2022 (GLOBE NEWSWIRE) — Caribou Biosciences, Inc. ( CRBU), a leading clinical-stage CRISPR genome-editing biopharmaceutical company, today announced the appointment of David L. Johnson to its board of directors. Mr. Johnson is a seasoned executive with 30 years of commercial and operational experience in the biopharmaceutical…

. 2022 Aug 1;1219:340031. doi: 10.1016/j.aca.2022.340031. Epub 2022 Jun 3. Affiliations Expand Affiliations 1 Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. 2 Pharmaceutical Research Center, Pharmaceutical Technology Institute,…

Multiplexed activation in mammalian cells using a split-intein CRISPR/Cas12a based synthetic transcription factor – Fingerprint — University of Edinburgh Research Explorer Sort by Weight Alphabetically Medicine & Life Sciences Inteins 100% Clustered Regularly Interspaced Short Palindromic Repeats 75% Acidaminococcus 64% Francisella 52% Transcription Factors 49% Cellular Reprogramming 42% Cell Biology…

Get instant alerts when news breaks on your stocks. Claim your 1-week free trial to StreetInsider Premium here. TUEBINGEN, Germany and HOUSTON and CAMBRIDGE, Mass., June 07, 2022 (GLOBE NEWSWIRE) — Immatics N.V. (Nasdaq: IMTX, “Immatics”), a clinical-stage biopharmaceutical company active in the discovery and development of T cell-redirecting cancer…

The clustered regularly interspaced short palindromic repeats-Cas (CRISPR-Cas) defensive system is an acquired mechanism in prokaryotes which is analogous to RNAi in eukaryotes [1]. The CRISPR machinery is a complex immune strategy that is continually evolving in the bacterial genome to accommodate the rapid changes in the nucleic acids of…

Mycoplasma pneumoniae can cause severe respiratory tract infections and extrapulmonary diseases, which pose a significant threat to the health of children. Diagnostic methods for M. pneumoniae include isolation and culture, antibody detection, fluorescence quantitative PCR, and so on, but there are various shortcomings in time, cost, convenience, and sensitivity. In…

The trans-cleavage activity of CRISPR/Cas12a has been widely used in biosensing applications. However, the lack of exploration on the fundamental properties of CRISPR/Cas12a not only discourages further in-depth studies of the CRISPR/Cas12a system but also limits the design space of CRISPR/Cas12a-based applications. Herein, a “RESET” effect (random extending sequences enhance…

30 Pages Posted: 17 May 2022 See all articles by Yulin Zhang Hubei University of Chinese Medicine Hubei University of Chinese Medicine Hubei Provincial Hospital of Traditional Chinese Medicine Hubei Provincial Hospital of Traditional Chinese Medicine Hubei University of Chinese Medicine…

Last Price $ Last Trade Change $ Change Percent % Open $ Prev Close $ High $ low $ 52 Week High $ 52 Week Low $ Market Cap PE Ratio Volume Exchange EDIT – Market Data & News Trade Editas Medicine Inc (NASDAQ: EDIT) has fallen $0.45 (4.05%) and …

. 2022 Apr;24(4):590-600. doi: 10.1038/s41556-022-00870-7. Epub 2022 Apr 12. Lucie Y Guo #  1   2 , Jing Bian #  3 , Alexander E Davis  4 , Pingting Liu  4 , Hannah R Kempton  3 , Xiaowei Zhang  3 , Augustine Chemparathy  3 , Baokun Gu  3 , Xueqiu Lin  3 , Draven A Rane  3 , Xiaoshu Xu  3 , Ryan M…

PDF.MPro – Full Text Journal Article: Exploiting the orthogonal CRISPR-Cas12a/Cas13a trans-cleavage for dual-gene virus detection using a handheld device. Home / Search / Abstract 34653714 (Page 1) Advertisement Find full text journal articles PMID: 34653714 (view PubMed database entry)DOI: 10.1016/j.bios.2021.113701 (read at publisher’s website ) Tian Tian, Zhiqiang Qiu, Yongzhong…

Abstract Deep learning has shown great promise in the prediction of the gRNA efficiency, which helps optimize the engineered gRNAs, and thus has greatly improved the usage of CRISPR-Cas systems in genome editing. However, the black box prediction of deep learning methods does not provide adequate explanation to the factors…

Although the CRISPR/Cas system has pioneered a new generation of analytical techniques, there remain many challenges in developing a label-free, accurate, and reliable CRISPR/Cas-based assay for reporting the levels of low abundance biomolecules in complex biological samples. Here, we reported a novel CRISPR-derived resonance Rayleigh scattering (RRS) amplification strategy and…

Multiplexed modulation of endogenous genes is crucial for sophisticated gene therapy and cell engineering. CRISPR–Cas12a systems enable versatile multiple-genomic-loci targeting by processing numerous CRISPR RNAs (crRNAs) from a single transcript; however, their low efficiency has hindered in vivo applications. Through structure-guided protein engineering, we developed a hyper-efficient Lachnospiraceae bacterium Cas12a…

Available online 9 April 2022, 131864 doi.org/10.1016/j.snb.2022.131864Get rights and content Highlights • We firstly introduce a general RPA-CRISPR/Cas12a sensing platform into fluorescent and electrochemical dual-signal output. • We firstly established an isothermal, rapid, specific and ultrasensitive RPA-CRISPR/Cas12a sensing platform for Brucella spp. detection. • This platform enabled rapid and accurate…

Tech ID: 32640 / UC Case 2022-071-0 Patent Status Patent Pending Brief Description The inventors engineered a set of LbCas12a mutants through rational design and directed evolution. The engineered mutants can function as efficient genome editors with minimized trans-activity. Suggested uses Suggested uses for the engineered LbCas12a mutants with minimized…

Available online 22 March 2022, 114207 doi.org/10.1016/j.bios.2022.114207Get rights and content Abstract The cytochrome c oxidase subunit III (COX III) gene is a powerful biomarker for the early diagnosis of acute kidney injury. However, current methods for COX III gene detection are usually laborious and time-consuming, with limited sensitivity. Herein, we…

The diagnosis and treatment of viral infections, especially those that cause large-scale outbreaks, is urgently needed, as the lack of these measures allows epidemics to progress. A new Biosafety and Health study discusses the use of the highly efficient clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) technology to…

[Courtesy of KAIST] SEOUL — Using genetic scissors technology, South Korean researchers have developed a technique capable of detecting the activation of a specific family of endonuclease enzymes that can cause DNA instability. The genetic technique will contribute to the development of the cure for acquired immunodeficiency syndrome, a life-threatening…

doi: 10.3389/fbioe.2022.845688. eCollection 2022. Affiliations Expand Affiliations 1 Provincial Key Laboratory for Transfusion-Transmitted Infectious Diseases, Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, China. 2 Clinical Laboratory Department, Yan’an Hospital Affiliated to Kunming Medical University, Kunming, China. 3 Department of Emergency, The Traditional…

In nature, microbes have evolved different systems to sense external stimuli. Synthetic biology approaches (1) repurpose these systems as biosensors to specifically and sensitively detect various targets of interest. Although various highly sensitive and specific laboratory-based analytical methods (including high-performance liquid chromatography and mass spectrometry) can detect small-molecule targets, they…

Background: In the context of the current pandemic caused by the novel coronavirus, molecular detection is not limited to the clinical laboratory, but also faces the challenge of the complex and variable real-time detection fields. A series of novel coronavirus events were detected in the process of food cold chain…

Get instant alerts when news breaks on your stocks. Claim your 1-week free trial to StreetInsider Premium here. BERKELEY, Calif., March 07, 2022 (GLOBE NEWSWIRE) — Caribou Biosciences, Inc. (Nasdaq: CRBU), a leading clinical-stage CRISPR genome-editing biopharmaceutical company, announced today that Rachel Haurwitz, Ph.D., Caribou’s president and chief executive officer,…

Editas Medicine, Inc. (EDIT) is a leading genome editing company engaged in translating the potential of the CRISPR/Cas12a and CRISPR/Cas9 genome editing platforms into a vigorous treatment pipeline for people with unmet medical needs. The price of EDIT stock during the regular trading on February 28, 2022, was $17.1 with…

CRISPR-Cas12a is an alternative class 2 gene-editing tool that may cause less off-target effects than the original Cas9 system. We have previously demonstrated that Cas12a attack with a single CRISPR RNA (crRNA) can neutralize all infectious HIV in an infected T cell line in cell culture. However, we demonstrated that…

Introduction About 700,000 people die from “superbugs” globally every year. Antibiotic abuse is the culprit of extensive spread of superbugs. Currently, carbapenemase-producing organisms are among the most important pathogens of hospital infections. Especially the plasmid-mediated highly transmissible carbapenem-resistant Enterobacterales (CRE) has become an important public health issue of global concern….

Every few months we highlight a subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they’ll make it easier for you to find and use the plasmids you need. If you’d ever…

Review doi: 10.3389/fgeed.2022.780238. eCollection 2022. Affiliations Expand Affiliations 1 Department of Plant Science and Landscape Architecture, University of Maryland, College Park, College Park, MD, United States. 2 College of Agriculture, Nanjing Agricultural University, Nanjing, China. 3 Integrated DNA Technologies, Coralville, IA, United States. 4 U.S. Horticultural Research Laboratory, USDA-Agricultural Research…

Sci-Hub | Cas9 versus Cas12a/Cpf1: Structure-function comparisons and implications for genome editing. Wiley Interdisciplinary Reviews: RNA, 9(5), e1481 | 10.1002/wrna.1481 ◂ ↓ save Swarts, D. C., & Jinek, M. (2018). Cas9 versus Cas12a/Cpf1: Structure-function comparisons and implications for genome editing. Wiley Interdisciplinary Reviews: RNA, 9(5), e1481. doi:10.1002/wrna.1481  10.1002/wrna.1481 Read more…

The mutation status of epidermal growth factor receptor (EGFR) exon 19 is of great importance for predicting sensitivity to tyrosine kinase inhibitors (TKIs) in the treatment of non-small-cell lung cancer (NSCLC). However, the development of simple, sensitive, and no-nonspecific amplification platforms for EGFR 19del detection in NSCLC remains a challenge….

BERKELEY, Calif., Feb. 07, 2022 (GLOBE NEWSWIRE) — Caribou Biosciences, Inc. (Nasdaq:CRBU), a leading clinical-stage CRISPR genome-editing biopharmaceutical company, announced today that Rachel Haurwitz, Ph.D., Caribou’s president and chief executive officer, will participate in a fireside chat at the SVB Leerink 11th Annual Global Healthcare Conference on Thursday, February 17th,…

W. Hong et al. (12) previously demonstrated the utility of a CRISPR-Cas12a system in the low-GC Gram-positive organism C. difficile (12). As C. difficile and E. faecium have similar codon usage, we utilized Cas12a placed under the control of the tetracycline-inducible promoter (Ptet) from pRPF185 on the pMTL82151 backbone (pDL1,…

Available online 29 January 2022, 123276 doi.org/10.1016/j.talanta.2022.123276Get rights and content Highlights • Using the signal amplification elements of TdT and CRISPR-Cas12a, an electrochemical aptasensor was introduced. • The biosensor indicated a vast linear detection range of 40 pM–150 nM with a LOD of 15 pM to cocaine. • The approach has…

Available online 13 January 2022, 113994 doi.org/10.1016/j.bios.2022.113994Get rights and content Highlights • A one-pot CRISPR/Cas13a-based visual biosensor for the rapid and low-cost nucleic acid detection is proposed. • With a newly developed tube-in-tube vessel used in the biosensor, aerosol cross contamination could be completely avoided. • The sensor can not…

. 2021 Dec 24;3(1):101072. doi: 10.1016/j.xpro.2021.101072. eCollection 2022 Mar 18. Affiliations Expand Affiliation 1 Department of Plant Pathology, Kansas State University, Manhattan, KS, USA. Free PMC article Item in Clipboard Jun Huang et al. STAR Protoc. 2021. Free PMC article Show details Display options Display options Format AbstractPubMedPMID . 2021 Dec…

Rapid and sensitive point-of-care detection of Leptospira by RPA-CRISPR/Cas12a targeting lipL32. PLOS Neglected Tropical Diseases ( IF 4.411 ) Pub Date : 2022-01-06 , DOI: 10.1371/journal.pntd.0010112 Sirawit Jirawannaporn,Umaporn…

When a patient receives a kidney transplant, doctors carefully monitor them for signs of rejection in several ways, including biopsy. However, this procedure is invasive and can only detect issues at a late stage. Now, researchers reporting in ACS’ Analytical Chemistry have developed a CRISPR-based assay that can sensitively and…

BERKELEY, Calif., Jan. 04, 2022 (GLOBE NEWSWIRE) — Caribou Biosciences, Inc. (Nasdaq:CRBU), a leading clinical-stage CRISPR genome-editing biopharmaceutical company, announced today that Rachel Haurwitz, Ph.D., Caribou’s president and chief executive officer, will present a corporate update at the 40th Annual J.P. Morgan Healthcare Conference on Thursday, January 13, 2022 at…

Available online 4 January 2022, 113941 doi.org/10.1016/j.bios.2021.113941Get rights and content Highlights • The sensor based on the label-free functional hairpin probe and the disordered cleavage of CRISPR/Cas12a was successfully constructed, and it can also accurately distinguish target and non-target genes successfully used for the detection of the Pax-5a gene. •…

Dawei Li received his Bachelor’s degree (2003) and Master’s degree (2006) from Lanzhou University and PhD (2013) from Nanyang Technological University. At present he is an associate professor in college of biology and the environment at Nanjing Forestry University. His current research interests mainly focus on the design and synthesis…

Abstract Methanococcus maripaludis is a fast-growing and genetically tractable methanogen. To become a useful host organism for the biotechnological conversion of CO2 and renewable hydrogen to fuels and value-added products, its product scope needs to be extended. Metabolic engineering requires reliable and efficient genetic tools, in particular for genome editing…

doi: 10.1093/nar/gkab1227. Online ahead of print. Regina Shebanova  1 , Natalia Nikitchina  1   2 , Nikita Shebanov  1 , Vladimir Mekler  3 , Konstantin Kuznedelov  3 , Egor Ulashchik  4 , Ruslan Vasilev  5   6 , Olga Sharko  4 , Vadim Shmanai  4 , Ivan Tarassov  2 , Konstantin Severinov  1   3   7 , Nina Entelis  2…

Although 2021 was the year of viruses for the entire world population, it was even more so for growers, who were confronted with the infamous Tomato Brown Rugose Fruit Virus (ToBRFV). It has been quite a ride – which is, unfortunately, not over just yet. In order to give you…

Nucleic acid analysis using ultrasensitive and simple methods is critically important for the early-stage diagnosis and treatment of diseases. The CRISPR/Cas proteins, guided by a single-stranded RNA have shown incredible capability for sequence-specific targeting and detection. Herein, in order to improve and expand the application of CRISPR/Cas technology to the…

EDIT-301 is in development as a transformative, one-time treatment for people living with transfusion-dependent beta thalassemia Editas Medicine will initiate a Phase 1/2 clinical trial in 2022 CAMBRIDGE, Mass., Dec. 20, 2021 (GLOBE NEWSWIRE) — Editas Medicine, Inc. (Nasdaq: EDIT), a leading genome editing company, today announced that the U.S….

doi: 10.3389/fpls.2021.740177. eCollection 2021. Affiliations Expand Affiliations 1 Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, China. 2 Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan, China. Free PMC article Item in Clipboard Mei Luo et al. Front Plant Sci. 2021. Free PMC article…

Available online 8 December 2021 doi.org/10.1016/j.nbt.2021.12.002Get rights and content Highlights • An efficient geminiviral vector was developed from Beet curly top virus with a wide host range. • The geminiviral vector delivered CRISPR/Cas components efficiently into plant cells. • A high frequency of both mutagenesis and complete HDR in plant…

CRISPR illustration. Credit: National Institutes of Health A compact and efficient CRISPR-Cas system, named CasMINI, could be broadly useful for cell-engineering and gene-therapy applications because it is easier to deliver into cells. The findings appear in a study that was published on September 3, 2021, in the journal Molecular Cell….

Data to include patient safety assessments and a preliminary analysis of secondary endpoints to evaluate biological activity Abstract selected for oral presentation on September 29 Company to host webcast investor event following the presentation on September 29 at 11:00 a.m. ET CAMBRIDGE, Mass., Sept. 07, 2021 (News) — Editas Medicine, Inc. (Nasdaq:…

The common analogy for CRISPR gene editing is that it works like molecular scissors, cutting out select sections of DNA. Stanley Qi, assistant professor of bioengineering at Stanford University, likes that analogy, but he thinks it’s time to reimagine CRISPR as a Swiss Army knife. “CRISPR can be as simple as…

Data to include patient safety assessments and a preliminary analysis of secondary endpoints to evaluate biological activity Abstract selected for oral presentation on September 29 Company to host webcast investor event following the presentation on September 29 at 11:00 a.m. ET CAMBRIDGE, Mass., Sept. 07, 2021 (GLOBE NEWSWIRE) — Editas Medicine, Inc….

Scientists led by a team at Stanford University have developed a compact, efficient CRISPR-Cas system, called CasMINI, which is about half the size of existing CRISPR-Cas systems, and which could have broad utility for gene therapy applications as well as cell engineering. The researchers confirmed in experiments that CasMINI could,…

The common analogy for CRISPR gene editing is that it works like molecular scissors, cutting out select sections of DNA. Stanley Qi, assistant professor of bioengineering at Stanford University, likes that analogy, but he thinks it’s time to reimagine CRISPR as a Swiss Army knife. “CRISPR can be as simple…

A compact and efficient CRISPR-Cas system, named CasMINI, could be broadly useful for cell-engineering and gene-therapy applications because it is easier to deliver into cells. The findings appear in a study publishing September 3 in the journal Molecular Cell. This is a critical step forward for CRISPR genome-engineering applications….

Why grab just a pair of scissors when you could get more functionality with a Swiss knife? A team of researchers at Stanford University think they’ve built such a tool to apply to CRISPR gene editing, which could help advance the platform and make it easier to deliver into cells. the…

INTRODUCTION Clustered regularly interspaced short palindromic repeats (CRISPR) are a family of DNA sequences, which constitute a primitive immune system that is responsible for protecting prokaryotic cells from phage infections. New York, Aug. 24, 2021 (GLOBE NEWSWIRE) — Reportlinker.com announces the release of the report “CRISPR Based Therapeutics Market by…

SLEEK enables high efficiency, multi-transgene knock-in of induced Pluripotent Stem Cells (iPSCs), T cells, and Natural Killer (NK) cells Data support SLEEK as an optimized approach to develop next generation cell therapy medicines CAMBRIDGE, Mass., Aug. 20, 2021 (GLOBE NEWSWIRE) — Editas Medicine, Inc. (Nasdaq: EDIT), a leading genome editing company, today…