Tag: Cas12a

Preparation and recognition mechanism study of an scFv targeting chloramphenicol for a hybridization chain reaction-CRISPR/Cas12a amplified fluoroimmunoassay

. 2024 Mar 8:1293:342283. doi: 10.1016/j.aca.2024.342283. Epub 2024 Jan 22. Affiliations Expand Affiliations 1 College of Food Science and Technology, Hebei Agricultural University, Baoding, 071001, China. 2 Medical College, Nankai University, Tianjin, 300500, China. Electronic address: wangshuo@nankai.edu.cn. 3 College of Food Science and Technology, Hebei Agricultural University, Baoding, 071001, China….

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Author Correction: Prime editing using CRISPR-Cas12a and circular RNAs in human cells

New Cornerstone Science Laboratory, Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China Ronghong Liang, Zixin He, Haocheng Zhu, Jiacheng Hu, Guanwen Liu, Meiyan Liu, Rui Zhang & Caixia Gao College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China Zixin He, Haocheng Zhu, Meiyan Liu & Caixia Gao Qi Biodesign, Beijing,…

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Structure-guided discovery of anti-CRISPR and anti-phage defense proteins

Identification of putative anti-crispr proteins using structural features To identify Acrs in phage genomes, we began by retrieving ~66.5 million proteins from Integrated Microbial Genomes Virus database (IMG/VR)37. We excluded large proteins because over 90% of known Acrs contain less than 200 amino acids38 (Fig. 1A, Supplementary Data 1). To reduce computational…

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CRISPR/Cas12a linked sandwich aptamer assay for sensitive detection of thrombin

Background: Thrombin is a serine protease and hemostasis regulator with multiple functions and recognized as an important biomarker for diseases, and sensitive detection of thrombin is of significance for clinical diagnostics and disease monitoring. Recently, the target-triggered nonspecific single-stranded deoxyribonuclease activity of CRISPR/Cas system is discovered, making it become a…

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Rolling circle amplification assisted CRISPR/Cas12a dual-cleavage photoelectrochemical biosensor for highly sensitive detection of miRNA-21

Background: MicroRNA-21 has been determined to be the only microRNA overexpressed in 11 types of solid tumors, making it an excellent candidate as a biomarker for disease diagnosis and therapy. Photoelectrochemical (PEC) biosensors have been widely used for quantification of microRNA-21. However, most PEC biosensing processes still suffer from some…

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CRISPR-Cas12a base editors confer efficient multiplexed genome editing in rice.

Addgene: CRISPR-Cas12a base editors confer efficient multiplexed genome editing in rice. More than 20 requests More than 50 requests More than 100 requests Item added to cart. Cart quantity updated. Limit per order. Item already in cart. Could not add item to cart. Skip to main content CRISPR-Cas12a base editors…

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Plug-and-play smart transistor bio-chips implementing point-of-care diagnosis of AMI with modified CRISPR/Cas12a system

. 2024 Feb 15:246:115909. doi: 10.1016/j.bios.2023.115909. Epub 2023 Dec 5. Affiliations Expand Affiliations 1 School of Laboratory Medicine, Hubei University of Chinese Medicine, 16 Huangjia Lake West Road, Wuhan, 430065, PR China; Department of Pharmacy, Renmin Hospital, Hubei University of Medicine, Shiyan, 442000, Hubei, PR China. 2 School of Laboratory…

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Enhancement and inactivation effect of CRISPR/Cas12a via extending hairpin activators for detection of transcription factors

Li Q, Song ZL, Zhang YX, Zhu LN, Yang Q, Liu XF, Sun XF, Chen XX, Kong RM, Fan GC, Luo XL (2023) Synergistic Incorporation of two ssDNA activators enhances the trans-cleavage of CRISPR/Cas12a. Anal Chem 95:8879–8888 Article  CAS  PubMed  Google Scholar  Ma JY, Wang SY, Du YC, Wang DX,…

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CRISPR-Cas12a-Enhanced Mass Spectrometric DNA Nanomachine for HPV-16 Detection in Human Serum

Cervical cancer is the second most harmful cancer to women, while human papillomavirus (HPV)-16 is the main cause of cervical cancer. Early virus screening can reduce the risk of death, but sometimes challenged by the low concentration and complicated matrix in physiological samples such as human serums. Herein, we report…

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Data from the development and evaluation of a CRISPR/Cas12a-based biosensor for the detection of DNA from Pseudogymnoascus destructans using samples collected from various species of bats across the United States, from 2018 to 2023

Reported here are the fluorescence data points recorded from CRISPR/Cas12a biosensor assays to detect the presence or absence of DNA from Pseudogymnoascus destructans (Pd), the causative fungal agent of white-nose syndrome in bats, from artificial controls, dermal swab, and guano samples. Ct values from qPCR experiments are also provided in…

Continue Reading Data from the development and evaluation of a CRISPR/Cas12a-based biosensor for the detection of DNA from Pseudogymnoascus destructans using samples collected from various species of bats across the United States, from 2018 to 2023

Biosensors | Free Full-Text | DNA Probes for Cas12a-Based Assay with Fluorescence Anisotropy Enhanced Due to Anchors and Salts

3.1. Design of Experiments To search optimal structures of ssDNA probes with anchors, we operated with the following assumptions: (1) the anchor should be an easily accessible compound, (2) it should be readily incorporated into the ssDNA probe, (3) it should provide a significant difference in FA before and after…

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Streamlining point-of-care assays | Nature Biomedical Engineering

When designing diagnostic assays intended for point-of-care use, more attention should be given to simplicity of operation. When it comes to assays for the detection of disease biomarkers, analytical performance typically makes the headline. An assay that misses a substantial percentage of samples positive for the biomarker or that singles…

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Editas Medicine and Vertex Pharmaceuticals Enter into Non-exclusive License Agreement for Cas9

Vertex Pharmaceuticals to obtain a non-exclusive license for Cas9 for CASGEVY™ (exagamglogene autotemcel) Agreement extends Editas Medicine’s cash runway into 2026 CAMBRIDGE, Mass., Dec. 13, 2023 (GLOBE NEWSWIRE) — Editas Medicine, Inc. (Nasdaq: EDIT), a clinical-stage genome editing company, today announced that the Company and Vertex Pharmaceuticals entered into a license…

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Nanoprobe with a barcode | EurekAlert!

Protein-splitting enzymes play an important role in many physiological processes. Such proteases are generally present in an inactive state, only becoming activated under certain conditions. Some are linked to diseases like infections or cancer, making it important to have methods that can selectively detect active proteases. In the journal Angewandte…

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Editas (EDIT) Licenses Cas9 Tool to Vertex, Stock Up 6%

Editas Medicine, Inc. EDIT announced entering into a licensing agreement with Vertex Pharmaceuticals VRTX for the development of the latter’s new sickle cell disease (SCD) gene therapy, Casgevy. Per the terms of the agreement, Vertex will receive a non-exclusive license to utilize Editas’ Cas9 gene editing technology for ex vivo gene editing medicines…

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Sci-Hub | Cas12a and Lateral Flow Strip-Based Test for Rapid and Ultrasensitive Detection of Spinal Muscular Atrophy. Biosensors, 11(5), 154

Sci-Hub | Cas12a and Lateral Flow Strip-Based Test for Rapid and Ultrasensitive Detection of Spinal Muscular Atrophy. Biosensors, 11(5), 154 | 10.3390/bios11050154 ◂ ↓ save Zhang, C., Li, Z., Chen, M., Hu, Z., Wu, L., Zhou, M., & Liang, D. (2021). Cas12a and Lateral Flow Strip-Based Test for Rapid and…

Continue Reading Sci-Hub | Cas12a and Lateral Flow Strip-Based Test for Rapid and Ultrasensitive Detection of Spinal Muscular Atrophy. Biosensors, 11(5), 154

Programmable Aptasensor for Regulating CRISPR/Cas12a Activity

CRISPR-mediated aptasensors have gained prevalence for detecting non-nucleic acid targets. However, there is an urgent need to develop an easily customizable design to improve the signal-to-noise ratio, enhance universality, and expand the detection range. In this article, we report a CRISPR-mediated programmable aptasensor (CPAS) platform. The platform includes single-stranded DNA…

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Caribou Biosciences Provides Regulatory Update on CB-010 Pivotal Plan with Phase 3 Trial Initiation Expected by YE 2024

Caribou Biosciences, Inc. — Caribou met with the FDA and reached alignment on a pivotal trial in 2L LBCL with CB-010 versus a comparator arm of immunochemotherapy followed by HDCT and ASCT — — ANTLER Phase 1 trial continues dose expansion enrollment; initial dose expansion data and RP2D expected Q2…

Continue Reading Caribou Biosciences Provides Regulatory Update on CB-010 Pivotal Plan with Phase 3 Trial Initiation Expected by YE 2024

Caribou Biosciences Provides Regulatory Update on CB-010

— Caribou met with the FDA and reached alignment on a pivotal trial in 2L LBCL with CB-010 versus a comparator arm of immunochemotherapy followed by HDCT and ASCT — — ANTLER Phase 1 trial continues dose expansion enrollment; initial dose expansion data and RP2D expected Q2 2024 — —…

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New tool to analyze blood platelets holds maj

A novel technique to test platelet function within a person’s blood sample is faster, easier and more precise than methods currently in use, an experimental study shows. Nature Biomedical Engineering published the research, led by scientists at Emory University. The researchers demonstrated the proof-of-concept for the technique, which provides the…

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A novel universal small-molecule detection platform based on antibody-controlled Cas12a switching

Molecular diagnostics play an important role in illness detection, prevention, and treatment, and are vital in point-of-care test. In this investigation, a novel CRISPR/Cas12a based small-molecule detection platform was developed using Antibody-Controlled Cas12a Biosensor (ACCBOR), in which antibody would control the trans-cleavage activity of CRISPR/Cas12a. In this system, small-molecule was…

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Foods | Free Full-Text | Isothermal Amplification and CRISPR/Cas12a-System-Based Assay for Rapid, Sensitive and Visual Detection of Staphylococcus aureus

1. Introduction Staphylococcus aureus [1], one of the top five foodborne pathogens, has a common and strong aggressiveness in humans and can secrete multiple toxic proteins (Pathogenic enterotoxins, Hemolysin, PVL) [1,2] which can cause bacteraemia, endocarditis, meningitis, toxic shock syndrome, pneumonia and other dangerous infectious diseases [3]. Moreover, the worldwide…

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Biosensors | Free Full-Text | CRISPR/Cas12a-Based Detection Platform for Early and Rapid Diagnosis of Scrub Typhus

1. Introduction Orientia tsutsugamushi (OT) is an obligate intracellular parasite bacteria and the causative agent of scrub typhus (ST), which is associated with acute febrile illness (AFI) [1] and transmitted by mites through an infected chigger bite (in the larval stage). This disease, which was earlier believed to be endemic…

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Electrochemical biosensing for E.coli detection based on triple helix DNA inhibition of CRISPR/Cas12a cleavage activity

Background: Escherichia coli (E.coli) is both a commensal and a foodborne pathogenic bacterium in the human gastrointestinal tract, posing significant potential risks to human health and food safety. However, one of the major challenges in E.coli detection lies in the preparation and storage of antibodies. In traditional detection methods, antibodies…

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A minimally invasive, field-applicable CRISPR/Cas biosensor to aid in the detection of Pseudogymnoascus destructans, the causative fungal agent of white-nose syndrome in bats

doi: 10.1111/1755-0998.13902. Online ahead of print. Affiliations Expand Affiliations 1 U.S. Geological Survey, Wetland and Aquatic Research Center, Gainesville, Florida, USA. 2 Washington Department of Fish and Wildlife, Olympia, Washington, USA. 3 Cherokee Nation System Solutions, Contractor to the U.S. Geological Survey, Gainesville, Florida, USA. Item in Clipboard Adam A…

Continue Reading A minimally invasive, field-applicable CRISPR/Cas biosensor to aid in the detection of Pseudogymnoascus destructans, the causative fungal agent of white-nose syndrome in bats

CRISPR/Cas12a-Responsive Hydrogels for Conjugation-Free and Universal Indicator Release in Colorimetric Detection

Recent advances have demonstrated the significant potential and advantages to repurpose existing point-of-care reactions/devices to realize portable detection of nonoriginal targets, e.g., pathogen genes. However, pursuing this aim usually requires protein indicator-nucleic acid conjugation via a covalent bond, which may bring drawbacks such as high cost, complicated procedure, and annoying…

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A Ratiometric Nonfluorescent CRISPR Assay Utilizing Cas12a-Induced Plasmid Supercoil Relaxation

%PDF-1.4 % 205 0 obj <> endobj 238 0 obj <>stream 2023-12-05T05:55:23Z 2023-12-06T10:01:21-08:00 2023-12-06T10:01:21-08:00 macOS Version 14.1.1 (Build 23B81) Quartz PDFContext application/pdf A Ratiometric Nonfluorescent CRISPR Assay Utilizing Cas12a-Induced Plasmid Supercoil Relaxation uuid:30a83dc1-1dd2-11b2-0a00-4e09275d6100 uuid:30a83dc9-1dd2-11b2-0a00-aa0000000000 endstream endobj 5 0 obj <> endobj 2 0 obj <> endobj 106 0 obj <> endobj…

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CRISPR-Cas12a-integrated transgenes in genomic safe harbors retain…

Abstract Discovery of genomic safe harbor sites (SHSs) is fundamental for multiple transgene integrations, such as reporter genes, chimeric antigen receptors (CARs), and safety switches, which are required for safe cell products for regenerative cell therapies and immunotherapies. Here we identified and characterized potential SHS in human cells. Using the…

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New FREM platform delivers 98% accuracy for malaria screening | The Guardian Nigeria News

Breakthrough has been recorded in malaria detection as the new FREM platform delivers 98 per cent accuracy for rapid screening, genotyping. In a recent study published in eBioMedicine, researchers developed the Flexible, Robust, Equipment-free Microfluidic (FREM) platform for malaria screening and Plasmodium species genotyping. Malaria, a worldwide health concern caused…

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In what ways does the CRISPR-Cas13a system enable efficient phage genome editing?

What are the molecular mechanisms of CRISPR-Cas immunity in bacteria?5 answersCRISPR-Cas systems in bacteria provide adaptive and hereditary immunity against foreign nucleic acids. The systems consist of CRISPR arrays containing short DNA fragments called spacers and Cas proteins that facilitate spacer acquisition, processing, and recognition of targeted nucleic acids. The…

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You Don’t Have to Pick a Winner in Gene-Editing. Do This Instead.

The pace of innovation in biotechnology is accelerating at a rapid clip. It took researchers 13 years to sequence the first full human genome. By contrast, it only took CRISPR Therapeutics (CRSP 4.57%) 10 years to bring their “molecular scissors” concept to market. With machine learning and artificial intelligence in…

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new FREM platform delivers 98% accuracy for rapid screening and genotyping

In a recent study published in eBioMedicine, researchers developed the Flexible, Robust, Equipment-free Microfluidic (FREM) platform for malaria screening and Plasmodium species genotyping. Study: A versatile microfluidic platform for malaria infection screening and Plasmodium species genotyping. Image Credit: nechaevkon/Shutterstock.com Background Malaria, a worldwide health concern caused by Plasmodium species, needs precise detection and genotyping…

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New Study Develops Innovative Microfluidic Platform for Malaria Screening and Genotyping

Researchers have made a breakthrough in malaria detection and genotyping with the development of the Flexible, Robust, Equipment-free Microfluidic (FREM) platform. The current diagnostic techniques for detecting and identifying malaria-causing Plasmodium species often face limitations in resource-limited settings. However, the FREM platform offers a versatile solution to these challenges. The…

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CRISPR-Cas12a exhibits metal-dependent specificity switching

Abstract Cas12a is the immune effector of type V-A CRISPR-Cas systems and has been co-opted for genome editing and other biotechnology tools. The specificity of Cas12a has been the subject of extensive investigation both in vitro and in genome editing experiments. However, in vitro studies have often been performed at…

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CRISPR Gene Editing: Cas9 and Beyond

Scientists use the CRISPR-Cas9 system to target a DNA sequence of interest near a protospacer-adjacent motif (PAM). The PAM initiates Cas9-DNA binding, a guide RNA (yellow) invades the double helix (blue) and hybridizes with the target DNA, and Cas9 (red) breaks the unwound double-stranded target DNA. iStock Meletios Verras Stay…

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Highly sensitive detection of Salmonella based on dual-functional HCR-mediated multivalent aptamer and amplification-free CRISPR/Cas12a system

Background: Salmonella infection severely threatens human health and causes substantial medical and financial concerns. Sensitive and specific detection of Salmonella in food samples is crucial but remains challenging. While some traditional assays for S. typhimurium are reliable, they suffer from various limitations, such as being time-consuming (culture-based methods), involving intricate…

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Improved CRISPR Diagnostic Technology Enhances Efficacy and Efficiency for Nucleic Acid Detection

– Advertisement – Efficacy and Efficiency of CRISPR Diagnostic Technology Improved Researchers have developed a new method that improves existing diagnostic technology for a more rapid, sensitive, and deployable approach to molecular diagnostics. The research, published in Nature Communications, unravels new potential for simple and sensitive nucleic acid detection in…

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CRISPR enhanced nanotweezers for ultra-precise DNA manipulation and detection

Nov 27, 2023 (Nanowerk News) Since the concept of optical trapping was first proposed in the 1960s and 1970s by Arthur Ashkin, researchers have pursued innovative techniques to precisely manipulate and analyze individual biomolecules like DNA. Such capabilities would revolutionize genetics, molecular biology, and medicine by enhancing our understanding of…

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In vivo imaging of mitochondrial DNA mutations using an integrated nano Cas12a sensor

Mutations in mitochondrial DNA (mtDNA) play critical roles in many human diseases. In vivo visualization of cells bearing mtDNA mutations is important for resolving the complexity of these diseases, which remains challenging. Here we develop an integrated nano Cas12a sensor (InCasor) and show its utility for efficient imaging of mtDNA…

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Empowering optical tweezers with ‘biometric eyes’

a, The diagrammatic sketch of the three components in the solution: DNA@AuNS conjugate, CRISPR/Cas12a complex, and target ssDNA. b, Optical setup, the BS, SPF, and TL are beam splitter, short pass filter, and tube lens (f=200 mm), respectively. Additional details of the setup are provided in the Materials and Methods…

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CRISPR-powered optothermal nanotweezers allow targeted manipulation of single DNA molecules

Nov 22, 2023 (Nanowerk News) For decades, researchers have sought ways to precisely manipulate and identify individual molecules like DNA in liquid environments. Such capabilities could revolutionize areas ranging from disease diagnosis to drug development. However, the randomness of molecular movements in fluids has hindered progress. Now, scientists from Shenzhen…

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New Breakthrough Enhances CRISPR Diagnostic Technology

Researchers at the University of Connecticut have made a significant breakthrough in improving the efficacy and efficiency of CRISPR diagnostic technology. Led by Professor Changchun Liu, the team has developed a new method that enhances the existing clustered regularly interspaced short palindromic repeats (CRISPR) technology, allowing for faster, more sensitive,…

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CRISPR/Cas12a toolbox for genome editing in Methanosarcina acetivorans

1Aalto University, Finland The final, formatted version of the article will be published soon. Notify me Receive an email when it is updated You just subscribed to receive the final version of the article Methanogenic archaea play an important role in the global…

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Researchers Improve Efficacy and Efficiency of CRISPR Diagnostic Technology

Changchun Liu, professor of Biomedical Engineering at UConn Health, has developed a new method that improves existing diagnostic technology for a more rapid, sensitive, and deployable approach to molecular diagnostics. The research, published in Nature Communications, unravels new potential for simple and sensitive nucleic acid detection in various diagnostic settings—including…

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An ultrasensitive Cd2+ detection biosensor based on DNAzyme and CRISPR/Cas12a coupled with hybridization chain reaction

. 2023 Dec 1:1283:341950. doi: 10.1016/j.aca.2023.341950. Epub 2023 Oct 23. Affiliations Expand Affiliations 1 Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of State Forestry and Grassland Administration on Subtropical Forest Biodiversity Conservation, College of Life Sciences, Nanjing Forestry University, Nanjing, 210037, China. 2 Jiangsu Key Laboratory for…

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Synthetic Protein-to-DNA Input Exchange for Protease Activity Detection Using CRISPR-Cas12a | Analytical Chemistry | ChemRxiv

Abstract We present a novel activity-based detection strategy for matrix metalloproteinase 2 (MMP2), a critical cancer protease biomarker, leveraging a mechanism responsive to the proteolytic activity of MMP2 and its integration with CRISPR-Cas12a-assisted signal amplification. We designed a chemical translator comprising two functional units — a peptide and a peptide…

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CRISPR/Cas12a trans-cleavage triggered by cleavage ligation of dumbbell DNA for specific detection of human 8-oxoguanine DNA glycosylase activity

Poetsch AR (2020) The genomics of oxidative DNA damage, repair, and resulting mutagenesis. Comput Struct Biotechnol J 18:207–219. doi.org/10.1016/j.csbj.2019.12.013 Article  CAS  PubMed  PubMed Central  Google Scholar  Ba X, Aguilera-Aguirre L, Rashid QT, Bacsi A, Radak Z, Sur S, Hosoki K, Hegde ML, Boldogh I (2014) The role of 8-oxoguanine DNA…

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Rapid Adeno-Associated Virus Genome Quantification with Amplification-Free CRISPR-Cas12a

Abstract Efficient manufacturing of recombinant Adeno-Associated Viral (rAAV) vectors to meet rising clinical demand remains a major hurdle. One of the most significant challenges is the generation of large amounts of empty capsids without the therapeutic genome. There is no standardized analytical method to accurately quantify the viral genes, and…

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Primate-specific ZNF808 is essential for pancreatic development in humans

Subjects The study was conducted in accordance with the Declaration of Helsinki and all subjects or their parents/guardian gave informed written consent for genetic testing. DNA testing and storage in the Beta Cell Research Bank was approved by the Wales Research Ethics Committee 5 Bangor (REC 17/WA/0327, IRAS project ID…

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Detection of Clostridium perfringens Using Novel Methods Based on Recombinase-Aided Amplification Assay-Assisted CRISPR/Cas12a System

Clostridium perfringens is a highly versatile pathogen of humans and animals. Rapid and sensitive detection methods for C. perfringens are urgently needed for the timely implementation of control. In this study, to provide novel promising methods for the detection of C. perfringens, two rapid, sensitive, and instrument-free C. perfringens detection…

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Rapid detection of avian influenza virus based on CRISPR-Cas12a

Background: Avian influenza (AI) is a disease caused by the avian influenza virus (AIV). These viruses spread naturally among wild aquatic birds worldwide and infect domestic poultry, other birds, and other animal species. Currently, real-time reverse transcription polymerase chain reaction (rRT-PCR) is mainly used to detect the presence of pathogens…

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Rapid detection of avian influenza virus based on CRISPR-Cas12a | Virology Journal

Materials Escherichia coli strains expressing the LbCas12a protein, plasmids containing the AIV M and NP genes, and H1–H16 subtypes of AIV, newcastle disease virus (NDV), infectious bursal disease virus (IBDV), and infectious bronchitis virus (IBV) were conserved in the State Key Laboratory of Harbin Veterinary Research Institute (HVRI), Chinese Academy…

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Detection of cellular traction forces via the force-triggered Cas12a-mediated catalytic cleavage of a fluorogenic reporter strand

Orr, A. W., Helmke, B. P., Blackman, B. R. & Schwartz, M. A. Mechanisms of mechanotransduction. Dev. Cell 10, 11–20 (2006). Article  CAS  PubMed  Google Scholar  Zhang, Y. et al. Platelet integrins exhibit anisotropic mechanosensing and harness piconewton forces to mediate platelet aggregation. Proc. Natl Acad. Sci. USA 115, 325–330…

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Establishment of RT-RPA-Cas12a assay for rapid and sensitive detection of human rhinovirus B | BMC Microbiology

Clinical samples Thirty nasopharyngeal aspirates were collected from individuals with symptoms of respiratory tract infections, and tested positive for HRV-A (5 clinical samples), HRV-B (20 clinical samples), or HRV-C-positive (5 clinical samples) based on the method, as previously described [27]. Ten other specimens were tested negative for HRV-B, including human…

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IJMS | Free Full-Text | CRISPR/Cas9 Landscape: Current State and Future Perspectives

1. Introduction Genome editing has taken a leading position among genome modification technologies in a short time and is now widely used in gene therapy. To date, there are three main systems for genome editing: zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and CRISPR/Cas nucleases. Genome editing has…

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LAMP assay coupled with a CRISPR/Cas12a system for the rapid and ultrasensitive detection of porcine circovirus-like virus in the field

Shulman LM, Davidson I. Viruses with circular single-stranded DNA genomes are everywhere! Annual Rev Virolog. 2017;4(1):159–80. doi.org/10.1146/annurev-virology-101416-041953. Article  CAS  Google Scholar  Yang K, Zhang M, Liu Q, Cao Y, Zhang W, Liang Y, Song X, Ji K, Shao Y, Qi K, Tu J. Epidemiology and evolution of emerging porcine circovirus-like…

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Development of a rapid, sensitive detection method for SARS-CoV-2 and influenza virus based on recombinase polymerase amplification combined with CRISPR-Cas12a assay

. 2023 Nov;95(11):e29215. doi: 10.1002/jmv.29215. Yuning Wang  1 , Liqiang Wu  1 , Xiaomei Yu  2 , Gang Wang  1 , Ting Pan  2 , Zhao Huang  2 , Ting Cui  2 , Tianxun Huang  1 , Zhentao Huang  1 , Libo Nie  2 , Chungen Qian  3 Affiliations Expand Affiliations 1 Department of Reagent Research and Development, Shenzhen YHLO…

Continue Reading Development of a rapid, sensitive detection method for SARS-CoV-2 and influenza virus based on recombinase polymerase amplification combined with CRISPR-Cas12a assay

Rapid in situ RNA imaging based on Cas12a thrusting strand displacement reaction | Nucleic Acids Research

Abstract RNA In situ imaging through DNA self-assembly is advantaged in illustrating its structures and functions with high-resolution, while the limited reaction efficiency and time-consuming operation hinder its clinical application. Here, we first proposed a new strand displacement reaction (SDR) model (Cas12a thrusting SDR, CtSDR), in which Cas12a could overcome…

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Caribou Biosciences Announces Appointment of Sundar Jagannath, MD, to its Scientific Advisory Board

Caribou Biosciences, Inc. — Dr. Jagannath is a renowned expert in the research and treatment of multiple myeloma — BERKELEY, Calif., Nov. 06, 2023 (GLOBE NEWSWIRE) —  Caribou Biosciences, Inc. (Nasdaq: CRBU), a leading clinical-stage CRISPR genome-editing biopharmaceutical company, today announced the appointment of Sundar Jagannath, MD, to its scientific advisory…

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Rapid and sensitive detection of Pseudomonas aeruginosa by isothermal amplification combined with Cas12a-mediated detection

P. aeruginosa is a common pathogenic bacterium, which seriously threaten human health12,13. In 2017, the World Health Organization (WHO) added P. aeruginosa to the short list of antibiotic-resistant pathogens, the so-called ESKAPE group of pathogens. Traditional pathogenic microbial detection methods require the isolation, culture, and biochemical identification of pathogenic bacteria,…

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RPA biosensors for rapid zoonoses screening

Introduction Since the emergence of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) in 2019, this coronavirus has spread to more than 200 countries, cumulatively infecting 672 million people and causing 6.84 million deaths. Moreover, the emergence of numerous variant strains (α, β, γ, δ, and Omicron) presents a serious challenge for…

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Detection of the Uveal Melanoma-Associated Mutation GNAQ Q209P from Liquid Biopsy Using CRISPR/Cas12a Technology

Uveal melanoma (UM) is a rare ocular tumor characterized by high metastasis risk and poor prognosis. The in-depth characterization of UM’s molecular profile is critical for better disease classification and prognosis. Furthermore, the development of detection tools to monitor UM evolution upon treatment is of great interest for designing optimal…

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Development of a separation platform comprising magnetic beads combined with the CRISPR/Cas12a system enabling ultrasensitive and rapid detection of miRNA-21

Lu J, Getz G, Miska EA, Alvarez-Saavedra E, Lamb J, Peck D, Sweet-Cordero A, Ebert BL, Mak RH, Ferrando AA, Downing JR, Jacks T, Horvitz HR, Golub TR (2005) MicroRNA expression profiles classify human cancers. Nature 4357043:834–838 Article  Google Scholar  Volinia S, Calin GA, Liu C-G, Ambs S, Cimmino A,…

Continue Reading Development of a separation platform comprising magnetic beads combined with the CRISPR/Cas12a system enabling ultrasensitive and rapid detection of miRNA-21

One-Step Reverse-Transcription Recombinase-Aided Amplification CRISPR/Cas12a-Based Lateral Flow Assay for Fast Field Screening and Accurate Differentiation of Four Major Tobamoviruses Infecting Tomato and Pepper

doi: 10.1021/acs.jafc.3c05268. Online ahead of print. Affiliations Expand Affiliations 1 Chinese Academy of Inspection and Quarantine, Beijing 100176, China. 2 State Key Laboratory of Agrobiotechnology and Key Laboratory of Pest Monitoring and Green Management-MOA, Department of Plant Pathology, China Agricultural University, 100193 Beijing, China. Item in Clipboard Zhenxing Zhao et al….

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Optical detection using CRISPR-Cas12a of Helicobacter pylori for veterinary applications

. 2023 Nov 1;190(11):455. doi: 10.1007/s00604-023-06037-x. Affiliations Expand Affiliations 1 MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China. 2 MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University,…

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Surveillance and genotyping of SARS-CoV-2 in sewage water by Cas12a-facilitated portable plasmonic biosensor

(2023, October 30) retrieved 30 October 2023 from phys.org/news/2023-10-surveillance-genotyping-sars-cov-sewage-cas12a-facilitated.html This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.Use this form if you have…

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Establishment and Application of CRISPR-Cas12a-Based Recombinase Polymerase Amplification and a Lateral Flow Dipstick and Fluorescence for the Detection and Distinction of Deformed Wing Virus Types A and B

Deformed wing virus (DWV) is one of the important pathogens of the honey bee (Apis mellifera), which consists of three master variants: types A, B, and C. Among them, DWV types A (DWV-A) and B (DWV-B) are the most prevalent variants in honey bee colonies and have been linked to…

Continue Reading Establishment and Application of CRISPR-Cas12a-Based Recombinase Polymerase Amplification and a Lateral Flow Dipstick and Fluorescence for the Detection and Distinction of Deformed Wing Virus Types A and B

Early and sensitive detection of pathogens fo

Article Highlight | 28-Oct-2023 Research image: Schematic representation of the portable fiber optic sensing platform for detecting SARS-CoV-2 in sewage water. view more  Credit: All rights reserved. Infectious diseases severely threaten public health and global biosafety. In addition to transmission through the air, pathogenic microorganisms have also been detected in environmental…

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Engineering a compact genome-editing tool

RESEARCH HIGHLIGHT 26 October 2023 CRISPR–Cas systems incorporating Streptococcus pyogenes Cas9 (SpCas9) and Acidaminococcus sp. Cas12a (AsCas12a) are commonly used genome-editing tools in human cells. However, these Cas enzymes are at the size limit for packaging in adeno-associated virus (AAV)-based delivery vehicles, which has hampered their applications. Writing in Cell,…

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Animals | Free Full-Text | RPA-CRISPR/Cas12a-Based Detection of Haemophilus parasuis

H. parasuis is a common pathogenic bacterium in the respiratory tract of pigs, which can cause widespread cellulose inflammation (also called Glässer’s disease) when the immunity of pigs is decreased. H. parasuis has fifteen serovars, and some clinical strains remain indistinguishable from serotypes. The vaccine’s cross-protection is poor among different…

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How CRISPR Companies Target Sickle Cell Anemia

A Crippling And Painful Disease Sickle Cell Disease (SCD) is a blood disease caused by a genetic mutation. This mutation creates abnormal hemoglobin, the protein carrying oxygen in the blood’s red cells. As a result, red cells are shaped like sickles and tend to get stuck in blood vessels, causing…

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CRISPR/Cas12a and primer-assisted rolling circle amplification integrated ultra-sensitive dual-signal sensing platform for EGFR 19 detection

. 2023 Oct 23:1279:341755. doi: 10.1016/j.aca.2023.341755. Epub 2023 Sep 6. Affiliations Expand Affiliations 1 Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering, College of Chongqing University, Chongqing, 400044, PR China. 2 Key Laboratory of Bio-Resource and Eco-environment…

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DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

Abstract We report a fast, easy-to-implement, highly sensitive, sequence-specific, and point-of-care (POC) DNA virus detection system, which combines recombinase polymerase amplification (RPA) and CRISPR/Cas12a system for trace detection of DNA viruses. Target DNA is amplified and recognized by RPA and CRISPR/Cas12a separately, which triggers the collateral cleavage activity of Cas12a…

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Entropy-driven reactions for controlling CRISPR/Cas12a and constructing an electrochemical biosensor for cardiac biomarkers detection

Lei Y-M, Xiao M-M, Li Y-T, Xu L, Zhang H, Zhang Z-Y et al (2017) Detection of heart failure-related biomarker in whole blood with graphene field effect transistor biosensor. Biosens Bioelectron 91:1–7 Article  CAS  PubMed  Google Scholar  Xin Y, Wang M, Liu M, Chen Y, Zhao H, Zhang P et…

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New Cas12a Enzymes Show Promise for Plant Gene Editing

Experts from the University of Maryland, USA, reported two new Cas12a enzymes, Ev1Cas12a and Hs1Cas12a, that are particularly efficient at editing plant genomes. These enzymes have been tested for gene editing in rice, tomato, and poplar plants and showed high accuracy and efficiency.   CRISPR-Cas12a is a powerful gene…

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Enhanced activity of CRISPR-Cas12a immobilized on hydrophilic metal: Organic frameworks for SARS-CoV-2 detection

Taking advantage of outstanding excellent target recognition accuracy and trans-cleavage ability, CRISPR/Cas12a system provides an alternative opportunity for designing fluorescence biosensors. To fully exploit the potential of the assay, we introduce here a method for stabilizing enzyme activity. Metal–organic frameworks (MOFs) are proposed to protect Cas12a from harsh environments. Herein,…

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CRISPR-Cas12a test strip (CRISPR/CAST) package: In-situ detection of Brucella from infected livestock | BMC Veterinary Research

Infections caused by Brucella have emerged as a considerable threat worldwide, particularly in vast amounts of livestock. It is essential to eradicate and control Brucella infections in livestock from the source. In addition, earlier detection and timely culling are equally important. Consequently, the screening used for livestock must be accurate, sensitive, specific, simple,…

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Genome Engineering Oxford (GEO) Facility

We also are actively involved in developing new gene editing methods and provide opportunities for collaborations on more extensive or challenging uses of CRISPR techniques such as development of new construct designs, application to whole organisms, development and use of pooled genome-wide CRISPR libraries. Services/Equipment available Engineered mammalian and bacterial…

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A new method for the detection of Mycobacterium tuberculosis based on the CRISPR/Cas system | BMC Infectious Diseases

Expression and purification of Cas12a protein The pMBP-LbCas12a recombinant plasmid used in this study was purchased from Addgene Global Plasmid Information Sharing Platform (Plasmid#113,431) and was 9653 bp in size. The recombinant plasmid was transformed into DH5α competent state and cultured at 37℃ for 6 h. IPTG was added at a final…

Continue Reading A new method for the detection of Mycobacterium tuberculosis based on the CRISPR/Cas system | BMC Infectious Diseases

A Crispr Cas12a-based DNAzyme visualization system for rapid non-electrically dependent detection of

Full Terms & Conditions of access and use can be found at www.tandfonline.com/action/journalInformation?journalCode=temi20 Emerging Microbes & Infections ISSN: (Print) (Online) Journal homepage: www.tandfonline.com/loi/temi20 A CRISPR/Cas12a-based DNAzyme visualization system for rapid, non-electrically dependent detection of Bacillus anthracis Dongshu Wang, Gang Chen, Yufei Lyu, Erling Feng, Li Zhu, Chao Pan, Weicai Zhang,…

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Infectious Disease Diagnosis and Pathogen Identification Platform Based on Multiplex Recombinase Polymerase Amplification-Assisted CRISPR-Cas12a System

Controlling and mitigating infectious diseases caused by multiple pathogens or pathogens with several subtypes require multiplex nucleic acid detection platforms that can detect several target genes rapidly, specifically, sensitively, and simultaneously. Here, we develop a detection platform, termed Multiplex Assay of RPA and Collateral Effect of Cas12a-based System (MARPLES), based…

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Living Magnetotactic Microrobots Based on Bacteria with a Surface-Displayed CRISPR/Cas12a System for Penaeus Viruses Detection

ACS Appl Mater Interfaces . 2023 Oct 9. doi: 10.1021/acsami.3c09690. Online ahead of print. Haoxiang Chen  1 , Tao Zhou  1 , Shuo Li  2 , Junya Feng  1 , Wenlong Li  1 , Lihuang Li  1 , Xi Zhou  1 , Miao Wang  1 , Fang Li  3 , Xueqin Zhao  2 , Lei Ren  1   4 Affiliations…

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Foods | Free Full-Text | Qualitative and Quantitative Detection of CRISPR-Associated Cas Gene in Gene-Edited Foods

1. Introduction Gene editing technology can effectively edit target genes and is therefore a valuable tool in biological research [1,2,3]. The third-generation gene editing technology, namely clustered regularly interspaced short palindromic repeats (CRISPR), is a defense system in bacteria. The presence of these repeats was first discovered in Escherichia coli…

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Programmable RNA detection with CRISPR-Cas12a

Santosh R. Rananaware, Emma K. Vesco, Grace M. Shoemaker, Swapnil S. Anekar, Luke Samuel W. Sandoval, Katelyn S. Meister, Nicolas C. Macaluso, Long T. Nguyen and Piyush K. Jain () Additional contact information Santosh R. Rananaware: University of Florida Emma K….

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AsCpf1/Cas12a (Strain BV3L6) Antibody | Cell Signaling Technology

Limited Uses Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer’s terms and conditions that are in addition to, or different from, those contained herein, unless separately…

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ISOTHERMAL RECOMBINANT POLYMERASE AMPLIFICATION AND CRIPSR(CAS12A) ASSAY DETECTION OF RENIBACTERIUM SALMONINARUM AS AN EXAMPLE FOR WILDLIFE PATHOGEN DETECTION IN ENVIRONMENTAL DNA SAMPLES

doi: 10.7589/JWD-D-22-00128. Online ahead of print. Affiliations Expand Affiliations 1 University of Washington, School of Marine and Environmental Affairs, 3737 Brooklyn Ave. NE, Seattle, Washington 98105, USA. 2 Wild EcoHealth LLC, Tacoma, Washington 98465, USA. 3 U.S. Geological Survey, Western Fisheries Research Center, 6505 Northeast 65th St., Seattle, Washington 98115,…

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a laboratory validation and evaluation study.

Abstract BACKGROUND: The 2022 outbreak of mpox (formerly known as monkeypox) led to the spread of monkeypox virus (MPXV) in over 110 countries, demanding effective disease management and surveillance. As current diagnostics rely largely on centralised laboratory testing, our objective was to develop a simple rapid point-of-care assay to detect…

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A CRISPR/Cas12a-SERS platform for amplification-free detection of African swine fever virus genes

African swine fever virus (ASFV) is a highly infectious and lethal agent in pigs and is classified as a “notifiable disease” by the World Organization for Animal Health (OIE) [1,2]. Although ASFV cannot infect humans, it is extremely difficult to prevent and control due to its multiple infection paths, which…

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Sci-Hub | CRISPR-Cas12a-Based Aptasensor for On-Site and Highly Sensitive Detection of Microcystin-LR in Freshwater. Environmental Science & Technolo

Sci-Hub | CRISPR-Cas12a-Based Aptasensor for On-Site and Highly Sensitive Detection of Microcystin-LR in Freshwater. Environmental Science & Technolo | 10.1021/acs.est.1c06733 ◂ ↓ save Kang, Y., Su, G., Yu, Y., Cao, J., Wang, J., & Yan, B. (2022). CRISPR-Cas12a-Based Aptasensor for On-Site and Highly Sensitive Detection of Microcystin-LR in Freshwater. Environmental…

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Compact Gene-Editing Enzyme Could Enable More Effective Clinical Therapies

Scientists headed by a team at the University of Tokyo have developed a new CRISPR-based gene-editing tool that they suggest could lead to better treatments for patients with genetic disorders. The tool is a version of the compact AsCas12f enzyme that incorporates mutations giving it the same effectiveness as the…

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Unmodificated stepless regulation of CRISPR/Cas12a multi-performance

As CRISPR technology is promoted to more fine-divided molecular biology applications, its inherent performance finds it increasingly difficult to cope with diverse needs in these different fields, and how to more accurately control the performance has become a key issue to develop CRISPR technology to a new stage. Herein, we…

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Innovative strategies of reprograming immune system cells

Introduction Cancer is well characterized by genomic instability resulting in structural alterations that build up with tumor progression.1,2 A subpopulation of undifferentiated cancer cells, known as cancer stem cells (CSCs) are found within the tumor bulk. These cells are responsible for cancer initiation, recurrence and show therapeutic resistance and these…

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ENHANCED EFFICIENCY OF RNA-GUIDED CAS12a VERSUS CAS9 TRANSGENE KNOCK-IN AND ACTIVITY AT A SCHISTOSOMA MANSONI GENOME SAFE HARBOR

Abstract Recently, we reported programmed Cas9 mediated insertion of a reporter gene into a gene safe harbor site; GSH1, of Schistosoma mansoni via homology-directed repair (HDR) using overlapping guide RNAs. Here, we report efficient and precise CRISPR/Cas12a-mediated homology-directed insertion of a 5′-C6-PEG10-modified double-stranded transgene bearing microhomology arms, 50nt length at…

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AbbVie Terminates Caribou CAR-T Contract in Culling of Cancer Pipeline

Pictured: AbbVie corporate office in Illinois/iStock, vzphotos AbbVie has walked away from its collaboration and license agreement with Caribou Biosciences, the Berkeley-based biotech announced in an SEC filing posted Tuesday.   AbbVie’s decision was a strategic move by the company “and was unrelated to Caribou’s performance under the agreement or…

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Advances in Cas12a-Based Amplification-Free Nucleic Acid Detection

In biomedicine, rapid and sensitive nucleic acid detection technology plays an important role in the early detection of infectious diseases. However, most traditional nucleic acid detection methods require the amplification of nucleic acids, resulting in problems such as long detection time, complex operation, and false-positive results. In recent years, clustered…

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Highly Sensitive Crispr/Cas12a Bioassay for Aflatoxin B1 by Single Metal Nanoparticle Counting by Chengchao Zhang, Xin Zhao, Zili Huang, Jianyu Hu, Rui Liu, Yi Lv :: SSRN

19 Pages Posted: 25 Sep 2023 Abstract CRISPR and Cas systems have gained great success in bioanalytical applications after the discovery of trans-cleavage activity. Combining with the functional aptamer sensors, the detecting signal transduction of non-nucleic acid targets was achieved with…

Continue Reading Highly Sensitive Crispr/Cas12a Bioassay for Aflatoxin B1 by Single Metal Nanoparticle Counting by Chengchao Zhang, Xin Zhao, Zili Huang, Jianyu Hu, Rui Liu, Yi Lv :: SSRN

Caribou Bioscience (CRBU) Announces Termination of Collaboration and License Agreement by AbbVie (ABBV)

On September 26, 2023, Caribou Bioscience, Inc (NASDAQ: CRBU) received notice from AbbVie Manufacturing Management Unlimited Company (NASDAQ: ABBV) that AbbVie has elected to terminate the Collaboration and License Agreement, as amended, by and between the Company and AbbVie, dated February 9, 2021 (the “Agreement”). AbbVie exercised its right to…

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Detection of Salmonella DNA and drug-resistance mutation by PCR-based CRISPR-lbCas12a system | AMB Express

Establishment of PCR-lbCas12 DETECTR The whole detection process is illustrated in Fig. 1a. According to the data of infected persons from the Medical Laboratory of Shenzhen Luohu People’s Hospital, Salmonella is always among the top 5 positive Gram-negative (G−) bacilli in both 2021 and 2020 (Fig. 1b). To obtain efficient and specific…

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Application of CRISPR-Cas12a technology in zebrafish to simulate beta-globin gene (HBB) mutations

Abstract Background: The human beta-globin (HBB) gene translated into the hemoglobin subunit beta (beta) of the hemoglobin protein. When mutated, it can lead to the blood disorder beta-thalassemia. Recently, the CRISPR-Cas12 technology has shown promising potential in treating different genetic abnormalities. Objectives: This study aimed to evaluate the feasibility of…

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A CRISPR-Cas12a-based platform facilitates the detection and serotyping of Streptococcus suis serotype 2

Streptococcus suis is a facultative anaerobic Gram-positive bacterium responsible for substantial economic losses in the worldwide swine industry. It can cause meningitis, septicemia, endocarditis, and sudden death in pigs. The bacterium can colonize the tonsil and nasal cavities of healthy pigs, with single or multiple serotypes possible in an individual…

Continue Reading A CRISPR-Cas12a-based platform facilitates the detection and serotyping of Streptococcus suis serotype 2