Tag: Cas13

(MENAFN– PR Newswire) DUBLIN, Jan. 8, 2024 /PRNewswire/ — The “Genome Editing Market – Global Industry Size, Share, Trends, Opportunity, and Forecast, 2018-2028F” report has been added to ResearchAndMarkets’s offering. The transformative power of genome editing continues to spur significant growth in the biotechnology sector, with the Global Genome Editing…

Kalkan, Ali Kerem; Palaz, Fahreddin; Sofija, Semeniuk; Elmousa, Nada; Ledezma, Yuri; Cachat, Elise; Rios-Solis, Leonardo; (2023) Improving recombinant protein production in CHO cells using the CRISPR-Cas system. Biotechnology Advances , 64 , Article 108115. 10.1016/j.biotechadv.2023.108115. Text Improving recombinant protein production in CHO cells using the CRISPR-Cas system.pdf – Accepted Version…

doi: 10.1093/nar/gkad1132. Online ahead of print. Affiliations Expand Affiliations 1 Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, Rochester, NY, USA. 2 Center for RNA Biology, School of Medicine and Dentistry, University of Rochester, Rochester, NY, USA. 3 Department of Biomedical Genetics, School of Medicine…

Scientists use the CRISPR-Cas9 system to target a DNA sequence of interest near a protospacer-adjacent motif (PAM). The PAM initiates Cas9-DNA binding, a guide RNA (yellow) invades the double helix (blue) and hybridizes with the target DNA, and Cas9 (red) breaks the unwound double-stranded target DNA. iStock Meletios Verras Stay…

By Andy Tay, PhD Overuse of antibiotics has accelerated the emergence of antimicrobial-resistant (AMR) bacterial species. The World Health Organization named antibacterial resistance as a top public health threat to humanity. In 2022, the Antimicrobial Resistance Collaborators reported that AMR bacteria directly caused 1.3 million deaths and was associated with…

Rayamajhee, B., Willcox, M. D., Henriquez, F. L., Petsoglou, C. & Carnt, N. Acanthamoeba keratitis: an increasingly common infectious disease of the cornea. Lancet Microbe 2, e345–e346 (2021). PubMed  Google Scholar  Guimaraes, A. J., Gomes, K. X., Cortines, J. R., Peralta, J. M. & Peralta, R. H. Acanthamoeba spp. as…

Orr, A. W., Helmke, B. P., Blackman, B. R. & Schwartz, M. A. Mechanisms of mechanotransduction. Dev. Cell 10, 11–20 (2006). Article  CAS  PubMed  Google Scholar  Zhang, Y. et al. Platelet integrins exhibit anisotropic mechanosensing and harness piconewton forces to mediate platelet aggregation. Proc. Natl Acad. Sci. USA 115, 325–330…

1. Introduction Genome editing has taken a leading position among genome modification technologies in a short time and is now widely used in gene therapy. To date, there are three main systems for genome editing: zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and CRISPR/Cas nucleases. Genome editing has…

Shulman LM, Davidson I. Viruses with circular single-stranded DNA genomes are everywhere! Annual Rev Virolog. 2017;4(1):159–80. doi.org/10.1146/annurev-virology-101416-041953. Article  CAS  Google Scholar  Yang K, Zhang M, Liu Q, Cao Y, Zhang W, Liang Y, Song X, Ji K, Shao Y, Qi K, Tu J. Epidemiology and evolution of emerging porcine circovirus-like…

P. aeruginosa is a common pathogenic bacterium, which seriously threaten human health12,13. In 2017, the World Health Organization (WHO) added P. aeruginosa to the short list of antibiotic-resistant pathogens, the so-called ESKAPE group of pathogens. Traditional pathogenic microbial detection methods require the isolation, culture, and biochemical identification of pathogenic bacteria,…

Introduction Since the emergence of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) in 2019, this coronavirus has spread to more than 200 countries, cumulatively infecting 672 million people and causing 6.84 million deaths. Moreover, the emergence of numerous variant strains (α, β, γ, δ, and Omicron) presents a serious challenge for…

Lu J, Getz G, Miska EA, Alvarez-Saavedra E, Lamb J, Peck D, Sweet-Cordero A, Ebert BL, Mak RH, Ferrando AA, Downing JR, Jacks T, Horvitz HR, Golub TR (2005) MicroRNA expression profiles classify human cancers. Nature 4357043:834–838 Article  Google Scholar  Volinia S, Calin GA, Liu C-G, Ambs S, Cimmino A,…

A recent study published in Nature Biomedical Engineering sheds light on the previously unknown ability of Cas13 to intrinsically target host RNA in mammalian cells. Cas13 is a member of the type-VI CRISPR-Cas13 systems, which have been widely utilized for various RNA-targeting applications. Cas13 is known to require a single…

Cas13 is capable of intrinsically targeting host RNA in mammalian cells through mechanisms that were not previously understood. Through transcript-specific mechanisms that are independent of the CRISPR RNA sequence and dynamically dependent on the conformational state of Cas13, Cas13 can also cleave host RNA. This effect is demonstrated by a…

Marketresearch.biz highlights that the growth of gene editing market is driven by increased genetically modified crop production and rising demand for synthetic genes in emerging markets, particularly for crop enhancement. CRISPR/Cas9 technology is often researchers’ top choice for crop development and modification. New York, Oct. 23, 2023 (GLOBE NEWSWIRE) —…

Abudayyeh, O. O. et al. C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector. Science 353, aaf5573 (2016). Article  PubMed  PubMed Central  Google Scholar  Terns, M. P. CRISPR-based technologies: impact of RNA-targeting systems. Mol. Cell 72, 404–412 (2018). Article  CAS  PubMed  PubMed Central  Google Scholar  Abudayyeh, O. O. et al….

Concentrating on ataxin-2 with RfxCas13d Due to its potential to affect TDP-43-associated toxicity20,25,26, ataxin-2 has emerged as a doubtlessly broadly relevant goal for ALS-FTD, as TDP-43 pathology is noticed in ~97% of ALS instances1,4,5 and ~45% FTD occurrences1,2,4. Given this, we sought to find out if RNA-targeting CRISPR effector proteins…

Originally described in: RNA editing with CRISPR-Cas13.Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180. PubMed Journal Articles Citing pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090 Articles PIE-seq: identifying RNA-binding protein targets by dual RNA-deaminase editing and sequencing. Ruan X, Hu K, Zhang…

Small and precise: These are the ideal characteristics for CRISPR systems, the Nobel-prize winning technology used to edit nucleic acids like RNA and DNA. Rice University scientists have described in detail the three-dimensional structure of one of the smallest known CRISPR-Cas13 systems used to shred or modify RNA and employed…

Small and precise: These are the ideal characteristics for CRISPR systems, the Nobel-prize winning technology used to edit nucleic acids like RNA and DNA. Emmanuel Osikpa (from left), Xue Sherry Gao, Xiangyu Deng, Jamie Smith, Seye J. Oladeji and Yang Gao (Photo by Jeff Fitlow/Rice University) Rice University scientists have…

Biotechnology has ushered in a new era of medical innovation, and at the forefront of this revolution stands CRISPR-Cas9 gene-editing technology. This groundbreaking development in biotechnology has the potential to reshape the landscape of healthcare as we know it. The impact on healthcare and various other fields is profound, offering…

Gray, M. W. Evolutionary origin of RNA editing. Biochemistry 51, 5235–5242 (2012). Article  CAS  PubMed  Google Scholar  Covello, P. & Gray, M. On the evolution of RNA editing. Trends Genet. 9, 265–268 (1993). Article  CAS  PubMed  Google Scholar  Yablonovitch, A. L., Deng, P., Jacobson, D. & Li, J. B. The…

Li, Y., Glass, Z., Huang, M., Chen, Z. Y. & Xu, Q. Ex vivo cell-based CRISPR/Cas9 genome editing for therapeutic applications. Biomaterials 234, 119711 (2020). Article  CAS  PubMed  PubMed Central  Google Scholar  Dong, W. & Kantor, B. Lentiviral vectors for delivery of gene-editing systems based on CRISPR/Cas: current state and…

CRISPR-based diagnostics offer numerous advantages, shifting clinical testing from lab settings to a patient-centric approach. Yet, until now, tests have only targeted DNA. Here, we share how our lab developed SAHARA, a method for RNA detection. Over the last decade, molecular diagnostics have undergone a transformative revolution with the help…

Surani MA, Barton SC, Norris ML. Development of reconstituted mouse eggs suggests imprinting of the genome during gametogenesis. Nature. 1984;308:548–50. Article  CAS  PubMed  Google Scholar  McGrath J, Solter D. Completion of mouse embryogenesis requires both the maternal and paternal genomes. Cell. 1984;37:179–83. Article  CAS  PubMed  Google Scholar  Barlow DP, Stoger…

Boualem, A., Dogimont, C. & Bendahmane, A. The battle for survival between viruses and their host plants. Curr. Opin. Virol. 17, 32–38 (2016). Article  CAS  PubMed  Google Scholar  Rashid, M. O., Wang, Y. & Han, C. G. Molecular detection of potato viruses in Bangladesh and their phylogenetic analysis. Plants 9,…

SINGAPORE – Scientists from the Genome Institute of Singapore (GIS) and the National University of Singapore’s Yong Loo Lin School of Medicine (NUS Medicine) have used a gene-editing tool to combat the virus that causes hand, foot and mouth disease (HFMD). The researchers say this technique could potentially lead to…

An important development which can potentially treat many diseases caused by RNA viruses A team of scientists from A*STAR’s Genome Institute of Singapore (GIS) and the Yong Loo Lin School of Medicine, National University of Singapore (NUS Medicine) has made an important breakthrough in the fight against RNA viruses that…

  Do you have a PhD in bioinformatics, viral genetics or a similar field? Keen to play a critical role in developing a novel solution to address viral outbreaks in livestock and aquaculture species? Join a multi-disciplinary, multi-CERC Fellow team in this exciting 3-year postdoctoral role   CSIRO Early Research…

The gold standard for rapid and specific detection of infectious disease agents is nucleic acid-based assays, such as polymerase chain reaction (PCR), but are costly, instrumentally expensive, and require highly trained personnel. Assays based on the CRISPR-Cas system have recently emerged as an alternative to PCR-based diagnostics. CRISPR detection relies…

Artificial intelligence can predict on- and off-target activity of CRISPR tools that target RNA instead of DNA, according to new research published in Nature Biotechnology. The study by researchers at New York University, Columbia Engineering, and the New York Genome Center combines a deep learning model with CRISPR screens to control the expression of human genes…

Artificial intelligence can predict on- and off-target activity of CRISPR tools that target RNA instead of DNA, according to new research published in Nature Biotechnology. The study by researchers at New York University, Columbia Engineering, and the New York Genome Center, combines a deep learning model with CRISPR screens to…

Researchers at New York University (NYU), Columbia University, and the New York Genome Center have developed an artificial intelligence (AI) platform that can predict on- and off-target activity of CRISPR tools that target RNA instead of DNA. The team paired a deep learning model with CRISPR screens to control the…

Artificial intelligence can predict on- and off-target activity of CRISPR tools that target RNA instead of DNA, according to new research published in Nature Biotechnology. The study by researchers at New York University, Columbia Engineering, and the New York Genome Center, combines a deep learning model with CRISPR screens to…

The discovery of Flap endonuclease 1 (FEN1) can be traced back to the 1960 s of the last centuries, Klett et al. discovered “Exonuclease VI” while studying Escherichia coli [1]. FEN1 is a structure-specific nuclease that precisely recognizes a double-flap DNA structure consisting of unpaired 5′-overhang and a single nucleotide 3′-flap,…

The global genome editing market size was USD 3.8 Billion in 2022 and is expected to reach USD 15.7 Billion by 2032, and register a rapid revenue CAGR of 15.2% during the forecast period. According to the latest report by Reports and Data, the global Genome Editing Market size was…

NEW YORK – Two teams of researchers at the Massachusetts Institute of Technology have demonstrated that some eukaryotic organisms have their own RNA-guided endonucleases, which likely share a common ancestor with certain Cas proteins. “Just when you think CRISPR is plateauing, there’s something new around the corner,” said Omar Abudayyeh,…

He G, Peng X, Wei S, Yang S, Li X, Huang M, et al. Exosomes in the hypoxic TME: from release, uptake and biofunctions to clinical applications. Mol Cancer. 2022;21:19. Article  PubMed  PubMed Central  Google Scholar  Downs-Canner SM, Meier J, Vincent BG, Serody JS. B cell function in the tumor…

The COVID-19 pandemic that emerged in early 2020 has affected all of us in many ways. Also for a group of people who are dedicated to research. Many of us have been wondering what we can do to help solve this tremendous global health problem, which has been progressing uncontrollably…

North American region is the largest shareholder in the global genome editing market, the increasing prevalence of genetic disorders and adoption of advanced gene therapy for the treatment of chronic diseases is expected to drive the market in the country during 2021-2028. Pune, India, June 23, 2023 (GLOBE NEWSWIRE) —…

The Insight Partners North American region is the largest shareholder in the global genome editing market, the increasing prevalence of genetic disorders and adoption of advanced gene therapy for the treatment of chronic diseases is expected to drive the market in the country during 2021-2028. Pune, India, June 23, 2023…

Pune, India, June 23, 2023 (GLOBE NEWSWIRE) — According to our latest study on “Genome Editing Market Size Report, Forecast to 2028 – COVID-19 Impact and Global Analysis – by Technology, Application, and End User,” the market size is projected to reach $16.98 billion by 2028; which was valued at…

LwaCas13a (previously known as LwaC2c2) is a crRNA-mediated RNA endonuclease derived from Leptotrichia wadeim. It can specifically recognize and cleave the RNA target with the PFS sequence. In addition, Cas13a also has the trans-cleavage activity, that is, when LwaCas13a binds crRNA and RNA target to form a ternary complex,…

Paragraf, a maker of electronic devices using semiconductor fabrication techniques, has just announced that their graphene-based biosensors can conduct multiomics analysis. Easily adapted to multiomics and multiplexed detection, this platform combines protein and nucleic acid recognition technology. Researchers from Keck Graduate Institute and Cardea Bio, which Paragraph acquired in May…

Summary CRISPR systems are prokaryotic adaptive immune systems that use RNA-guided Cas nucleases to recognize and destroy foreign genetic elements. To overcome CRISPR immunity, bacteriophages have evolved diverse families of anti-CRISPR proteins (Acrs). Recently, Lin et al. (2020) described the discovery and characterization of 7 Acr families (AcrVIA1–7) that inhibit…

The CRISPR Revolution There are hundreds and even thousands of diseases that have a root cause in genetics. Many of these are to this day untreatable or poorly treated. And most are either heavily incapacitating or deadly. While some gene modification technology has been around since the 1990s, they were…

Introduction Phytophthora sojae is among the most destructive pathogens of soybean (Glycine max). An estimated annual worldwide loss of US$1–2 billion is caused by P. sojae, which was first identified as a soybean pathogen in the 1950s in Indiana, Ohio, and North Carolina (Schmitthenner, 1985; Tyler, 2007; Dorrance, 2018). The…

Golubnitschaja O, Baban B, Boniolo G, Wang W, Bubnov R, Kapalla M, et al. Medicine in the early twenty-first century: paradigm and anticipation – EPMA position paper 2016. EPMA J. 2016;7:23. Article  PubMed  PubMed Central  Google Scholar  Kropp M, Golubnitschaja O, Mazurakova A, Koklesova L, Sargheini N, Vo T-TKS, et…

NEW YORK – Oxford Nanopore Technologies said on Thursday that it is rolling out a new direct RNA sequencing kit that the company promises will “significantly” increase the raw read accuracy as well as data output. While Oxford Nanopore has been known for its relatively rapid product turnover for DNA applications,…

Abstract The Na-K-2Cl cotransporter (NKCC1) is considered an attractive drug target in the Central nervous system (CNS) for treating various CNS disorders. However, the specific role of NKCC1 in different types following injury within the CNS is not well understood due to its expression in multiple cell types. Additionally, there…

Cite As: Krohannon A, Srivastava M, Rauch S, Srivastava R, Dickinson BC, Janga SC. CASowary: CRISPR-Cas13 guide RNA predictor for transcript depletion. BMC Genomics. 2022;23(1):172. Published 2022 Mar 2. doi:10.1186/s12864-022-08366-2 Abstract: Background: Recent discovery of the gene editing system – CRISPR (Clustered Regularly Interspersed Short Palindromic Repeats) associated proteins (Cas),…

Tapper EB, Curry MP. Hepatitis Caused by Other Viruses Handbook of Liver Disease, 2018: p. 78. Sarin SK, Kumar M. Viral hepatitis A, in Molecular Pathology of Liver Diseases. Springer; 2011. pp. 527–52. Qu B, Brown RJ. Strategies to inhibit Hepatitis B Virus at the transcript level. Viruses. 2021;13(7):1327. Article  CAS …

Reports And Data Genome editing market size was USD 3.8 Billion in 2022 and is expected to reach USD 15.7 Billion by 2032, and CAGR of 15.2% during the forecast period The global genome editing market size was USD 3.8 Billion in 2022 and is expected to reach USD 15.7…

MicroRNAs (miRNAs) are small noncoding RNAs consisting of approximately 20–25 nucleotides. As a kind of transcription regulation factors, miRNAs perform post-transcriptional repression by binding to the 3′UTR of target in the seed region, which is considered as a typical mode of miRNA-mediated gene regulation [[1], [2], [3]]. And they play…

CRISPR claimed scientific fame for its ability to quickly and accurately edit genes. But, at the core, CRISPR systems are immune systems that help bacteria protect themselves from viruses by targeting and destroying viral DNA and RNA. A new study published in Science reveals a previously unrecognized player in one…

Following the first reports of Severe fever with thrombocytopenia syndrome virus (SFTSV) during 2009 in Central China [1], there have been outbreaks reported in over 20 Chinese provinces in subsequent years [2], as well as SFTSV cases reported in Japan [3] and Korea [4]. The early, non-specific clinical symptoms of…

Detection of microbial cell-free DNA (cfDNA) circulating in the bloodstream has emerged as a promising new approach for diagnosing infection. Microbial diagnostics based on cfDNA require assays that can detect rare and highly fragmented pathogen nucleic acids. We now report WATSON (Whole-genome Assay using Tiled Surveillance Of Nucleic acids), a…

Almazán C, González-Álvarez VH, Fernández de Mera IG, Cabezas-Cruz A, Rodríguez-Martínez R, De la Fuente J (2016) Molecular identification and characterization of Anaplasma platys and Ehrlichia canis in dogs in Mexico. Ticks Tick Borne Dis 7:276–283. doi.org/10.1016/j.ttbdis.2015.11.002 Article  PubMed  Google Scholar  Aman R, Mahas A, Marsic T, Hassan N, Mahfouz…

Acknowledgement of CountryCSIRO acknowledges the Traditional Owners of the land, sea and waters, of the area that we live and work on across Australia. We acknowledge their continuing connection to their culture and pay our respects to their Elders past and present. View our vision towards reconciliation The Opportunity Do…

Cell culture HEK293T, MEF-1, and U87 cell lines were used in this research. HEK293T was purchased from ATCC. MEF-1, and U87 cell lines were gifts from Muredach P. Reilly, Peter A. Sims, respectively. HEK293T, MEF-1, and U87 cells were cultured in DMEM with 4.5 g/L D-Glucose, supplemented with 10% fetal bovine…

Affiliations Expand Affiliations 1 Laboratory of Experimental Virology, Department of Medical Microbiology, Amsterdam UMC, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands. 2 Department of Molecular and Cell Biology, National Center of Biotechnology (CNB-CSIC), Campus Universidad Autónoma de Madrid, 28049 Madrid, Spain. Free PMC article Item in…

Omar Abudayyeh, PhD, McGovern fellow at MIT’s McGovern Institute for Brain Research A recently patented genome editing tool called PASTE holds genuine promise for expanding the universe of treatable genetic diseases. The approach combines elements of CRISPR and prime editing with a pair of enzymes designed to enable the integration…

Doudna JA, Charpentier E. Genome editing. The new frontier of genome engineering with CRISPR-Cas9. Science. 2014;346(6213):1258096. Article  PubMed  Google Scholar  Doudna JA. The promise and challenge of therapeutic genome editing. Nature. 2020;578(7794):229–36. Article  CAS  PubMed  PubMed Central  Google Scholar  Nambiar TS, Baudrier L, Billon P, Ciccia A. CRISPR-based genome editing…

Figure 1. Replication cycle of SARS-CoV-2 and genome organization. (A) The SARS-CoV-2 replication cycle. Binding of the viral S protein to the ACE-2 receptor triggers SARS-CoV-2 infection and the plus genomic RNA (+gRNA) is translated into polyprotein (pp)1a and pp1ab. These proteins are proteolytically cleaved to generate 16 non-structural proteins…

Levine AJ, Finlay CA, Hinds PW (2004) P53 is a tumor suppressor gene. Cell 116:S67–S69 Article  CAS  PubMed  Google Scholar  Sigal A, Rotter V (2000) Oncogenic mutations of the p53 tumor suppressor: the demons of the guardian of the genome. Can Res 60:6788–6893 CAS  Google Scholar  Sammons MA, Nguyen T-AT,…

Introduction Maize (Zea mays L.) is one of the most important cereal crops in the world. In 2011, a large outbreak of maize lethal necrosis disease (MLND) occurred in the Southern Rift Valley of Kenya (Wangai et al., 2012), caused up to 100% losses in maize yield, and affected the income…

Sherlock Biosciences, a company engineering biology to bring next-generation lab-quality diagnostic testing to the point of need, has announced that the U.S. Patent and Trademark Office (USPTO) has granted a patent for the diagnostic use of the Cas12 enzyme. Sherlock has exclusive U.S. rights to the patent from Shanghai-based Tolo…

Bryan Dechairo, PhD, Sherlock Biosciences president and CEO Sherlock Biosciences has gained U.S. rights to a patent for diagnostic use of a CRISPR system based on the smaller Cas12 enzyme as single RNA-guided endonuclease. Sherlock—whose co-founders include CRISPR pioneer Feng Zhang, PhD, of the Broad Institute—says its exclusive U.S. rights to…

Sherlock Biosciences said on Tuesday that the U.S. Patent and Trademark Office (USPTO) has granted a patent for the diagnostic use of the Cas12 enzyme, an integral part of the firm’s diagnostic testing technology. Sherlock has exclusive U.S. rights to the patent from Shanghai-based Tolo Biotech, as well as to…

Preeminent IP position gives Sherlock freedom to operate and commercialize across multiple CRISPR business avenues WATERTOWN, Mass., Feb. 21, 2023 /PRNewswire/ — Sherlock Biosciences, a company engineering biology to bring next-generation lab-quality diagnostic testing to the point of need, today announced that the U.S. Patent and Trademark Office (USPTO) has…

Dr. Wenzhen Duan (left), Professor of Psychiatry and Behavioural Science at Johns Hopkins University School of Medicine, and Dr. Gene Yeo (right), Professor of Cellular and Molecular Medicine at the University of California San Diego, have created a novel CRISPR-Cas13d based therapy for Huntington’s Disease that depletes mutant Huntingtin transcripts…

Work by Adler et al.2 describes a 2-step in vitro technique for efficient genome editing of phages involving the broad-spectrum bacterial CRISPR–Cas13a. Cas13 effectors are RNA-guided RNA nucleases with catalytic activity against 2 higher eukaryotic and prokaryotic nucleotide-binding domains. The authors elegantly demonstrated how the Cas13a system from Leptotrichia buccalis…

● Description Recombinant L. buccalis LbuCas13a (type VI-A CRISPR-associated RNA-guided ribonuclease Cas13a), tag free. Cas13a is an RNA-guided endonuclease that belongs to the class 2 type VI CRISPR-Cas system. When activated, Cas13a induces collateral cleavage of nearby non-targeted RNAs in a nonspecific manner. ● Product Data Gallery 4-20% SDS-PAGE Coomassie…

doi: 10.1002/mco2.208. eCollection 2023 Feb. Zongzhi Liu  1   2   3 , Xiang Gao  4 , Chuanwen Kan  2   5 , Lingyu Li  1   2   3 , Yuan Zhang  6 , Yibo Gao  7 , Shengyuan Zhang  4 , Liangji Zhou  6 , Hui Zhao  6   8   9 , Mingkun Li  2  …

Gene Editing Hype Gene editing has for a while been hailed as the new frontier in medicine. The peak enthusiasm for investors on this topic was in early 2020, with the related stocks having cool off since. No matter the market sentiment, gene editing is still a big deal for…

Abdelmohsen, K., Panda, A.C., Munk, R., Grammatikakis, I., Dudekula, D.B., De, S., Kim, J., Noh, J.H., Kim, K.M., Martindale, J.L. et al., 2017. Identification of HuR target circular RNAs uncovers suppression of PABPN1 translation by CircPABPN1. RNA Biol. 14, 361-369 Aktas,…

Background: The CRISPR-Cas13 system is an RNA-guided RNA-targeting system and has been widely used in transcriptome engineering with potentially important clinical applications. However, it is still controversial whether Cas13 exhibits collateral activity in mammalian cells. Results: Here, we find that knocking down gene expression using RfxCas13d in the adult brain…

Introduction Tuberculosis (TB), caused by Mycobacterium tuberculosis (MTB) complex, remains one of the leading infectious causes of death worldwide, with an estimated 1.6 million TB deaths in 2021 (World Health Organization, 2022). Rapid and accurate TB diagnosis is critical to ensure timely initiation of anti-TB therapy (Walzl et al., 2018)….

Sherlock Biosciences has bought global molecular diagnostics company Sense Biodetection based out of Cambridge, U.K., to help it reach its goal of making diagnostics easy to use, cheap, disposable, and good for the environment. Sherlock’s purchase of Sense—which launched its fully integrated molecular diagnostic test that provides laboratory-quality results in…

Hampton, H. G., Watson, B. N. J. & Fineran, P. C. The arms race between bacteria and their phage foes. Nature 577, 327–336 (2020). Article  CAS  Google Scholar  Makarova, K. S. et al. Evolution and classification of the CRISPR-Cas systems. Nat. Rev. Microbiol. 9, 467–477 (2011). Article  CAS  Google Scholar …

A Brief History of CRISPR CRISPR are genetic sequences found in bacteria, which use them as a defense mechanism against viruses called bacteriophages. It essentially targets the virus’s genetic material to stop its propagation. CRISPR molecules can be used together with the Cas9 protein (Cas mean CRISPR Associated Protein) to…

Acknowledgement of CountryCSIRO acknowledges the Traditional Owners of the land, sea and waters, of the area that we live and work on across Australia. We acknowledge their continuing connection to their culture and pay our respects to their Elders past and present. View our vision towards reconciliation The Opportunity Do…

CRISPR, the Nobel Prize-winning gene-editing technology, is poised to once again have a profound impact on the fields of microbiology and medicine. A team led by CRISPR pioneer Jennifer Doudna and her longtime collaborator Jill Banfield has developed a clever tool to edit the genomes of bacteria-infecting viruses called bacteriophages…

7.3.1Type VI-A (Cas13a)In Cas13a, direct repeat region of crRNA is composed of 5–6 bp stem using Watsonand Crick base pairing with an unpaired 2 nt bulge (AA or AC) present at 30end ofstem, while loop region of stem contains 7–9 nt depending upon homologs ofCas13a. In addition, an 8–12 nt…

An engineered CRISPR-based method that finds RNA from SARS-CoV-2, the virus that causes COVID-19, promises to make testing for that and other diseases fast and easy, researchers report. The researchers further engineered the RNA-editing CRISPR-Cas13 system to boost their power for detecting minute amounts of the SARS-CoV-2 virus in biological…

doi: 10.1038/s41589-022-01135-y. Online ahead of print. Jie Yang #  1 , Yang Song #  2   3 , Xiangyu Deng  4 , Jeffrey A Vanegas  1 , Zheng You  1 , Yuxuan Zhang  2   3 , Zhengyan Weng  2   3 , Lori Avery  5 , Kevin D Dieckhaus  6 , Advaith Peddi  4 , Yang Gao  7 , Yi Zhang  8…

Abstract The success of CRISPR-mediated gene perturbation studies is highly dependent on the quality of gRNAs, and several tools have been developed to enable optimal gRNA design. However, these tools are not all adaptable to the latest CRISPR modalities or nucleases, nor do they offer comprehensive annotation methods for advanced…

In vitro reconstitution of Escherichia coli CRISPR-Cas3 interference E. coli CRISPR-Cas3 is generally well-characterized type I CRISPR complexes in vitro and in vivo32,33,37,38. However, recombinant EcoCas3 protein is difficult to purify because of poor solubility and propensity to aggregate at 37 °C25,26,30,39. Co-expression of HtpG chaperon40 and/or low temperature growth at…

DUBLIN–(BUSINESS WIRE)–The “Genome Editing Market Forecast to 2028 – COVID-19 Impact and Global Analysis By Technology, Application, and End User” report has been added to ResearchAndMarkets.com‘s offering. The genome editing market is projected to reach US$ 16,986.69 million by 2028 from US$ 5,199.40 million in 2021; it is expected to…

Don’t invest in stories. Don’t invest in a company before they have meaningful revenues. Don’t invest in drug developers that have a great deal of regulatory risk. These are all rules we need to break if we want exposure to a technology that allows mankind to start playing with the…

Introduction The clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated (Cas) protein systems naturally exist in prokaryotes. They are RNA-mediated defense mechanisms to protect bacteria and archaea from invading nucleic acids (Barrangou et al., 2007). CRISPR–Cas systems are classified into two classes (1 and 2). Class 2 CRISPR–Cas require a single…

Last year, researchers at MIT’s McGovern Institute for Brain Research discovered and characterized Cas7-11, the first CRISPR enzyme capable of making precise, guided cuts to strands of RNA without harming cells in the process. Now, working with collaborators at the University of Tokyo, the same team has revealed that Cas7-11…

Objectives CRISPR-Cas13a system-based nucleic acid detection methods are reported to have rapid and sensitive DNA detection. However, the screening strategy for crRNAs that enables CRISPR-Cas13a single-base resolution DNA detection of human pathogens remains unclear. Methods A combined rational design and target mutation-anchoring CRISPR RNA (crRNA) screening strategy was proposed. Results…

The diagnosis and treatment of viral infections, especially those that cause large-scale outbreaks, is urgently needed, as the lack of these measures allows epidemics to progress. A new Biosafety and Health study discusses the use of the highly efficient clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) technology to…

Abramson, J. S. et al. Transcend NHL 001: immunotherapy with the CD19-directed CAR T-cell product JCAR017 results in high complete response rates in relapsed or refractory B-cell non-Hodgkin lymphoma. Blood 128, 4192–4192 (2016). Google Scholar  Shifrut, E. et al. Genome-wide CRISPR screens in primary human T cells reveal key regulators…

In nature, microbes have evolved different systems to sense external stimuli. Synthetic biology approaches (1) repurpose these systems as biosensors to specifically and sensitively detect various targets of interest. Although various highly sensitive and specific laboratory-based analytical methods (including high-performance liquid chromatography and mass spectrometry) can detect small-molecule targets, they…

The Telomere-to-Telomere Consortium is sequencing whole chromosomes.Credit: Adrian T. Sumner/SPL From gene editing to protein-structure determination to quantum computing, here are seven technologies that are likely to have an impact on science in the year ahead. Fully finished genomes Roughly one-tenth of the human genome remained uncharted when genomics researchers…

24 January 2022 A hidden component of the CRISPR/Cas3 genome editing system, which is essential for it to work, has been discovered by scientists. The CRISPR/Cas3 family of genome editing systems is similar to other CRISPR families but can uniquely make larger and more accurate changes to DNA (see BioNews…

Abstract Summary The CRISPR-Cas13 system is an RNA-guided RNA-targeting system, and has been widely used in transcriptome engineering with potentially important clinical applications. However, it is still controversial whether Cas13 exhibits collateral activity in mammalian cells. Here, we found that knocking down gene expression using RfxCas13d in the adult brain…

McGovern Fellows Jonathan Gootenberg (left) and Omar Abudayyeh in their lab. Credit: Caitlin Cunningham Researchers at MIT’s McGovern Institute for Brain Research have discovered a bacterial enzyme that they say could expand scientists’ CRISPR toolkit, making it easy to cut and edit RNA with the kind of precision that, until…