Tag: Cas13

Summary CRISPR systems are prokaryotic adaptive immune systems that use RNA-guided Cas nucleases to recognize and destroy foreign genetic elements. To overcome CRISPR immunity, bacteriophages have evolved diverse families of anti-CRISPR proteins (Acrs). Recently, Lin et al. (2020) described the discovery and characterization of 7 Acr families (AcrVIA1–7) that inhibit…

The CRISPR Revolution There are hundreds and even thousands of diseases that have a root cause in genetics. Many of these are to this day untreatable or poorly treated. And most are either heavily incapacitating or deadly. While some gene modification technology has been around since the 1990s, they were…

Introduction Phytophthora sojae is among the most destructive pathogens of soybean (Glycine max). An estimated annual worldwide loss of US$1–2 billion is caused by P. sojae, which was first identified as a soybean pathogen in the 1950s in Indiana, Ohio, and North Carolina (Schmitthenner, 1985; Tyler, 2007; Dorrance, 2018). The…

Golubnitschaja O, Baban B, Boniolo G, Wang W, Bubnov R, Kapalla M, et al. Medicine in the early twenty-first century: paradigm and anticipation – EPMA position paper 2016. EPMA J. 2016;7:23. Article  PubMed  PubMed Central  Google Scholar  Kropp M, Golubnitschaja O, Mazurakova A, Koklesova L, Sargheini N, Vo T-TKS, et…

NEW YORK – Oxford Nanopore Technologies said on Thursday that it is rolling out a new direct RNA sequencing kit that the company promises will “significantly” increase the raw read accuracy as well as data output. While Oxford Nanopore has been known for its relatively rapid product turnover for DNA applications,…

Abstract The Na-K-2Cl cotransporter (NKCC1) is considered an attractive drug target in the Central nervous system (CNS) for treating various CNS disorders. However, the specific role of NKCC1 in different types following injury within the CNS is not well understood due to its expression in multiple cell types. Additionally, there…

Cite As: Krohannon A, Srivastava M, Rauch S, Srivastava R, Dickinson BC, Janga SC. CASowary: CRISPR-Cas13 guide RNA predictor for transcript depletion. BMC Genomics. 2022;23(1):172. Published 2022 Mar 2. doi:10.1186/s12864-022-08366-2 Abstract: Background: Recent discovery of the gene editing system – CRISPR (Clustered Regularly Interspersed Short Palindromic Repeats) associated proteins (Cas),…

Tapper EB, Curry MP. Hepatitis Caused by Other Viruses Handbook of Liver Disease, 2018: p. 78. Sarin SK, Kumar M. Viral hepatitis A, in Molecular Pathology of Liver Diseases. Springer; 2011. pp. 527–52. Qu B, Brown RJ. Strategies to inhibit Hepatitis B Virus at the transcript level. Viruses. 2021;13(7):1327. Article  CAS …

Reports And Data Genome editing market size was USD 3.8 Billion in 2022 and is expected to reach USD 15.7 Billion by 2032, and CAGR of 15.2% during the forecast period The global genome editing market size was USD 3.8 Billion in 2022 and is expected to reach USD 15.7…

MicroRNAs (miRNAs) are small noncoding RNAs consisting of approximately 20–25 nucleotides. As a kind of transcription regulation factors, miRNAs perform post-transcriptional repression by binding to the 3′UTR of target in the seed region, which is considered as a typical mode of miRNA-mediated gene regulation [[1], [2], [3]]. And they play…

CRISPR claimed scientific fame for its ability to quickly and accurately edit genes. But, at the core, CRISPR systems are immune systems that help bacteria protect themselves from viruses by targeting and destroying viral DNA and RNA. A new study published in Science reveals a previously unrecognized player in one…

Following the first reports of Severe fever with thrombocytopenia syndrome virus (SFTSV) during 2009 in Central China [1], there have been outbreaks reported in over 20 Chinese provinces in subsequent years [2], as well as SFTSV cases reported in Japan [3] and Korea [4]. The early, non-specific clinical symptoms of…

Detection of microbial cell-free DNA (cfDNA) circulating in the bloodstream has emerged as a promising new approach for diagnosing infection. Microbial diagnostics based on cfDNA require assays that can detect rare and highly fragmented pathogen nucleic acids. We now report WATSON (Whole-genome Assay using Tiled Surveillance Of Nucleic acids), a…

Almazán C, González-Álvarez VH, Fernández de Mera IG, Cabezas-Cruz A, Rodríguez-Martínez R, De la Fuente J (2016) Molecular identification and characterization of Anaplasma platys and Ehrlichia canis in dogs in Mexico. Ticks Tick Borne Dis 7:276–283. doi.org/10.1016/j.ttbdis.2015.11.002 Article  PubMed  Google Scholar  Aman R, Mahas A, Marsic T, Hassan N, Mahfouz…

Acknowledgement of CountryCSIRO acknowledges the Traditional Owners of the land, sea and waters, of the area that we live and work on across Australia. We acknowledge their continuing connection to their culture and pay our respects to their Elders past and present. View our vision towards reconciliation The Opportunity Do…

Cell culture HEK293T, MEF-1, and U87 cell lines were used in this research. HEK293T was purchased from ATCC. MEF-1, and U87 cell lines were gifts from Muredach P. Reilly, Peter A. Sims, respectively. HEK293T, MEF-1, and U87 cells were cultured in DMEM with 4.5 g/L D-Glucose, supplemented with 10% fetal bovine…

Affiliations Expand Affiliations 1 Laboratory of Experimental Virology, Department of Medical Microbiology, Amsterdam UMC, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands. 2 Department of Molecular and Cell Biology, National Center of Biotechnology (CNB-CSIC), Campus Universidad Autónoma de Madrid, 28049 Madrid, Spain. Free PMC article Item in…

Omar Abudayyeh, PhD, McGovern fellow at MIT’s McGovern Institute for Brain Research A recently patented genome editing tool called PASTE holds genuine promise for expanding the universe of treatable genetic diseases. The approach combines elements of CRISPR and prime editing with a pair of enzymes designed to enable the integration…

Doudna JA, Charpentier E. Genome editing. The new frontier of genome engineering with CRISPR-Cas9. Science. 2014;346(6213):1258096. Article  PubMed  Google Scholar  Doudna JA. The promise and challenge of therapeutic genome editing. Nature. 2020;578(7794):229–36. Article  CAS  PubMed  PubMed Central  Google Scholar  Nambiar TS, Baudrier L, Billon P, Ciccia A. CRISPR-based genome editing…

Figure 1. Replication cycle of SARS-CoV-2 and genome organization. (A) The SARS-CoV-2 replication cycle. Binding of the viral S protein to the ACE-2 receptor triggers SARS-CoV-2 infection and the plus genomic RNA (+gRNA) is translated into polyprotein (pp)1a and pp1ab. These proteins are proteolytically cleaved to generate 16 non-structural proteins…

Levine AJ, Finlay CA, Hinds PW (2004) P53 is a tumor suppressor gene. Cell 116:S67–S69 Article  CAS  PubMed  Google Scholar  Sigal A, Rotter V (2000) Oncogenic mutations of the p53 tumor suppressor: the demons of the guardian of the genome. Can Res 60:6788–6893 CAS  Google Scholar  Sammons MA, Nguyen T-AT,…

Introduction Maize (Zea mays L.) is one of the most important cereal crops in the world. In 2011, a large outbreak of maize lethal necrosis disease (MLND) occurred in the Southern Rift Valley of Kenya (Wangai et al., 2012), caused up to 100% losses in maize yield, and affected the income…

Sherlock Biosciences, a company engineering biology to bring next-generation lab-quality diagnostic testing to the point of need, has announced that the U.S. Patent and Trademark Office (USPTO) has granted a patent for the diagnostic use of the Cas12 enzyme. Sherlock has exclusive U.S. rights to the patent from Shanghai-based Tolo…

Bryan Dechairo, PhD, Sherlock Biosciences president and CEO Sherlock Biosciences has gained U.S. rights to a patent for diagnostic use of a CRISPR system based on the smaller Cas12 enzyme as single RNA-guided endonuclease. Sherlock—whose co-founders include CRISPR pioneer Feng Zhang, PhD, of the Broad Institute—says its exclusive U.S. rights to…

Sherlock Biosciences said on Tuesday that the U.S. Patent and Trademark Office (USPTO) has granted a patent for the diagnostic use of the Cas12 enzyme, an integral part of the firm’s diagnostic testing technology. Sherlock has exclusive U.S. rights to the patent from Shanghai-based Tolo Biotech, as well as to…

Preeminent IP position gives Sherlock freedom to operate and commercialize across multiple CRISPR business avenues WATERTOWN, Mass., Feb. 21, 2023 /PRNewswire/ — Sherlock Biosciences, a company engineering biology to bring next-generation lab-quality diagnostic testing to the point of need, today announced that the U.S. Patent and Trademark Office (USPTO) has…

Dr. Wenzhen Duan (left), Professor of Psychiatry and Behavioural Science at Johns Hopkins University School of Medicine, and Dr. Gene Yeo (right), Professor of Cellular and Molecular Medicine at the University of California San Diego, have created a novel CRISPR-Cas13d based therapy for Huntington’s Disease that depletes mutant Huntingtin transcripts…

Work by Adler et al.2 describes a 2-step in vitro technique for efficient genome editing of phages involving the broad-spectrum bacterial CRISPR–Cas13a. Cas13 effectors are RNA-guided RNA nucleases with catalytic activity against 2 higher eukaryotic and prokaryotic nucleotide-binding domains. The authors elegantly demonstrated how the Cas13a system from Leptotrichia buccalis…

● Description Recombinant L. buccalis LbuCas13a (type VI-A CRISPR-associated RNA-guided ribonuclease Cas13a), tag free. Cas13a is an RNA-guided endonuclease that belongs to the class 2 type VI CRISPR-Cas system. When activated, Cas13a induces collateral cleavage of nearby non-targeted RNAs in a nonspecific manner. ● Product Data Gallery 4-20% SDS-PAGE Coomassie…

doi: 10.1002/mco2.208. eCollection 2023 Feb. Zongzhi Liu  1   2   3 , Xiang Gao  4 , Chuanwen Kan  2   5 , Lingyu Li  1   2   3 , Yuan Zhang  6 , Yibo Gao  7 , Shengyuan Zhang  4 , Liangji Zhou  6 , Hui Zhao  6   8   9 , Mingkun Li  2  …

Gene Editing Hype Gene editing has for a while been hailed as the new frontier in medicine. The peak enthusiasm for investors on this topic was in early 2020, with the related stocks having cool off since. No matter the market sentiment, gene editing is still a big deal for…

Abdelmohsen, K., Panda, A.C., Munk, R., Grammatikakis, I., Dudekula, D.B., De, S., Kim, J., Noh, J.H., Kim, K.M., Martindale, J.L. et al., 2017. Identification of HuR target circular RNAs uncovers suppression of PABPN1 translation by CircPABPN1. RNA Biol. 14, 361-369 Aktas,…

Background: The CRISPR-Cas13 system is an RNA-guided RNA-targeting system and has been widely used in transcriptome engineering with potentially important clinical applications. However, it is still controversial whether Cas13 exhibits collateral activity in mammalian cells. Results: Here, we find that knocking down gene expression using RfxCas13d in the adult brain…

Introduction Tuberculosis (TB), caused by Mycobacterium tuberculosis (MTB) complex, remains one of the leading infectious causes of death worldwide, with an estimated 1.6 million TB deaths in 2021 (World Health Organization, 2022). Rapid and accurate TB diagnosis is critical to ensure timely initiation of anti-TB therapy (Walzl et al., 2018)….

Sherlock Biosciences has bought global molecular diagnostics company Sense Biodetection based out of Cambridge, U.K., to help it reach its goal of making diagnostics easy to use, cheap, disposable, and good for the environment. Sherlock’s purchase of Sense—which launched its fully integrated molecular diagnostic test that provides laboratory-quality results in…

Hampton, H. G., Watson, B. N. J. & Fineran, P. C. The arms race between bacteria and their phage foes. Nature 577, 327–336 (2020). Article  CAS  Google Scholar  Makarova, K. S. et al. Evolution and classification of the CRISPR-Cas systems. Nat. Rev. Microbiol. 9, 467–477 (2011). Article  CAS  Google Scholar …

A Brief History of CRISPR CRISPR are genetic sequences found in bacteria, which use them as a defense mechanism against viruses called bacteriophages. It essentially targets the virus’s genetic material to stop its propagation. CRISPR molecules can be used together with the Cas9 protein (Cas mean CRISPR Associated Protein) to…

Acknowledgement of CountryCSIRO acknowledges the Traditional Owners of the land, sea and waters, of the area that we live and work on across Australia. We acknowledge their continuing connection to their culture and pay our respects to their Elders past and present. View our vision towards reconciliation The Opportunity Do…

CRISPR, the Nobel Prize-winning gene-editing technology, is poised to once again have a profound impact on the fields of microbiology and medicine. A team led by CRISPR pioneer Jennifer Doudna and her longtime collaborator Jill Banfield has developed a clever tool to edit the genomes of bacteria-infecting viruses called bacteriophages…

7.3.1Type VI-A (Cas13a)In Cas13a, direct repeat region of crRNA is composed of 5–6 bp stem using Watsonand Crick base pairing with an unpaired 2 nt bulge (AA or AC) present at 30end ofstem, while loop region of stem contains 7–9 nt depending upon homologs ofCas13a. In addition, an 8–12 nt…

An engineered CRISPR-based method that finds RNA from SARS-CoV-2, the virus that causes COVID-19, promises to make testing for that and other diseases fast and easy, researchers report. The researchers further engineered the RNA-editing CRISPR-Cas13 system to boost their power for detecting minute amounts of the SARS-CoV-2 virus in biological…

doi: 10.1038/s41589-022-01135-y. Online ahead of print. Jie Yang #  1 , Yang Song #  2   3 , Xiangyu Deng  4 , Jeffrey A Vanegas  1 , Zheng You  1 , Yuxuan Zhang  2   3 , Zhengyan Weng  2   3 , Lori Avery  5 , Kevin D Dieckhaus  6 , Advaith Peddi  4 , Yang Gao  7 , Yi Zhang  8…

Abstract The success of CRISPR-mediated gene perturbation studies is highly dependent on the quality of gRNAs, and several tools have been developed to enable optimal gRNA design. However, these tools are not all adaptable to the latest CRISPR modalities or nucleases, nor do they offer comprehensive annotation methods for advanced…

In vitro reconstitution of Escherichia coli CRISPR-Cas3 interference E. coli CRISPR-Cas3 is generally well-characterized type I CRISPR complexes in vitro and in vivo32,33,37,38. However, recombinant EcoCas3 protein is difficult to purify because of poor solubility and propensity to aggregate at 37 °C25,26,30,39. Co-expression of HtpG chaperon40 and/or low temperature growth at…

DUBLIN–(BUSINESS WIRE)–The “Genome Editing Market Forecast to 2028 – COVID-19 Impact and Global Analysis By Technology, Application, and End User” report has been added to ResearchAndMarkets.com‘s offering. The genome editing market is projected to reach US$ 16,986.69 million by 2028 from US$ 5,199.40 million in 2021; it is expected to…

Don’t invest in stories. Don’t invest in a company before they have meaningful revenues. Don’t invest in drug developers that have a great deal of regulatory risk. These are all rules we need to break if we want exposure to a technology that allows mankind to start playing with the…

Introduction The clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated (Cas) protein systems naturally exist in prokaryotes. They are RNA-mediated defense mechanisms to protect bacteria and archaea from invading nucleic acids (Barrangou et al., 2007). CRISPR–Cas systems are classified into two classes (1 and 2). Class 2 CRISPR–Cas require a single…

Last year, researchers at MIT’s McGovern Institute for Brain Research discovered and characterized Cas7-11, the first CRISPR enzyme capable of making precise, guided cuts to strands of RNA without harming cells in the process. Now, working with collaborators at the University of Tokyo, the same team has revealed that Cas7-11…

Objectives CRISPR-Cas13a system-based nucleic acid detection methods are reported to have rapid and sensitive DNA detection. However, the screening strategy for crRNAs that enables CRISPR-Cas13a single-base resolution DNA detection of human pathogens remains unclear. Methods A combined rational design and target mutation-anchoring CRISPR RNA (crRNA) screening strategy was proposed. Results…

The diagnosis and treatment of viral infections, especially those that cause large-scale outbreaks, is urgently needed, as the lack of these measures allows epidemics to progress. A new Biosafety and Health study discusses the use of the highly efficient clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) technology to…

Abramson, J. S. et al. Transcend NHL 001: immunotherapy with the CD19-directed CAR T-cell product JCAR017 results in high complete response rates in relapsed or refractory B-cell non-Hodgkin lymphoma. Blood 128, 4192–4192 (2016). Google Scholar  Shifrut, E. et al. Genome-wide CRISPR screens in primary human T cells reveal key regulators…

In nature, microbes have evolved different systems to sense external stimuli. Synthetic biology approaches (1) repurpose these systems as biosensors to specifically and sensitively detect various targets of interest. Although various highly sensitive and specific laboratory-based analytical methods (including high-performance liquid chromatography and mass spectrometry) can detect small-molecule targets, they…

The Telomere-to-Telomere Consortium is sequencing whole chromosomes.Credit: Adrian T. Sumner/SPL From gene editing to protein-structure determination to quantum computing, here are seven technologies that are likely to have an impact on science in the year ahead. Fully finished genomes Roughly one-tenth of the human genome remained uncharted when genomics researchers…

24 January 2022 A hidden component of the CRISPR/Cas3 genome editing system, which is essential for it to work, has been discovered by scientists. The CRISPR/Cas3 family of genome editing systems is similar to other CRISPR families but can uniquely make larger and more accurate changes to DNA (see BioNews…

Abstract Summary The CRISPR-Cas13 system is an RNA-guided RNA-targeting system, and has been widely used in transcriptome engineering with potentially important clinical applications. However, it is still controversial whether Cas13 exhibits collateral activity in mammalian cells. Here, we found that knocking down gene expression using RfxCas13d in the adult brain…

McGovern Fellows Jonathan Gootenberg (left) and Omar Abudayyeh in their lab. Credit: Caitlin Cunningham Researchers at MIT’s McGovern Institute for Brain Research have discovered a bacterial enzyme that they say could expand scientists’ CRISPR toolkit, making it easy to cut and edit RNA with the kind of precision that, until…

The gene-editing tool CRISPR is moving toward the market, promising better tests, disease cures — and maybe even a woolly mammoth. The big picture: CRISPR is already a historic scientific achievement, but we’re just now entering the moment when it will begin to impact patients and possibly the planet. Driving…

Scientists, typically, are on their own after their post-doc ends. Sometimes, albeit rarely, they team up, blending their ideas, money, and people. Connie Cepko, PhD, and Cliff Tabin, PhD, pioneered the idea of a lab partnership, spending the last few decades working together in their shared lab at Harvard Medical…

INTRODUCTION Clustered regularly interspaced short palindromic repeats (CRISPR) are a family of DNA sequences, which constitute a primitive immune system that is responsible for protecting prokaryotic cells from phage infections. New York, Aug. 24, 2021 (GLOBE NEWSWIRE) — Reportlinker.com announces the release of the report “CRISPR Based Therapeutics Market by…