Tag: CAS13A

The epidermal growth factor receptor (EGFR), a member of the epidermal growth factor receptor (HER) family, is closely related to the activation of cell signaling pathways, including cell proliferation and apoptosis protection [1], [2]. Studies have been proved that EGFR is over-expressed in various tumors, especially in lung adenocarcinoma. This…

Summary CRISPR systems are prokaryotic adaptive immune systems that use RNA-guided Cas nucleases to recognize and destroy foreign genetic elements. To overcome CRISPR immunity, bacteriophages have evolved diverse families of anti-CRISPR proteins (Acrs). Recently, Lin et al. (2020) described the discovery and characterization of 7 Acr families (AcrVIA1–7) that inhibit…

Introduction Phytophthora sojae is among the most destructive pathogens of soybean (Glycine max). An estimated annual worldwide loss of US$1–2 billion is caused by P. sojae, which was first identified as a soybean pathogen in the 1950s in Indiana, Ohio, and North Carolina (Schmitthenner, 1985; Tyler, 2007; Dorrance, 2018). The…

Golubnitschaja O, Baban B, Boniolo G, Wang W, Bubnov R, Kapalla M, et al. Medicine in the early twenty-first century: paradigm and anticipation – EPMA position paper 2016. EPMA J. 2016;7:23. Article  PubMed  PubMed Central  Google Scholar  Kropp M, Golubnitschaja O, Mazurakova A, Koklesova L, Sargheini N, Vo T-TKS, et…

Thanks to a novel tool that detects bacteria in saliva using SHERLOCK, the evolution of CRISPR, you may soon be able to leave the dentist after a checkup with more comprehensive information about the health of your mouth. The accurate tool is much faster than existing methods and may lead…

doi: 10.1016/j.virusres.2023.199130. Online ahead of print. Affiliations Expand Affiliations 1 Viral Hemorrhagic Fevers Research Unit, CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, 200031, China; University of Chinese Academy of Sciences, Beijing, 100049, China. 2 Institute of Pathogenic Microorganisms, Guangdong Provincial…

Tapper EB, Curry MP. Hepatitis Caused by Other Viruses Handbook of Liver Disease, 2018: p. 78. Sarin SK, Kumar M. Viral hepatitis A, in Molecular Pathology of Liver Diseases. Springer; 2011. pp. 527–52. Qu B, Brown RJ. Strategies to inhibit Hepatitis B Virus at the transcript level. Viruses. 2021;13(7):1327. Article  CAS …

Liang Y, Lin H, Zou L, Deng X, Tang S. Biosens Bioelectron. 2022;205:114098. Article  CAS  PubMed  PubMed Central  Google Scholar  Telenti A, Hodcroft EB, Robertson DL. Cold Spring Harb Perspect Med. 2022;12:a041390. Article  CAS  PubMed  Google Scholar  Shrestha LB, Foster C, Rawlinson W, Tedla N, Bull RA. Reviews in Medical…

Abstract Public healthcare demands effective and pragmatic diagnostic tools to address the escalating challenges in infection management in resource-limited areas. Recent advance in CRISPR-based biosensing promises the development of next-generation tools for disease diagnostics, including point-of-care (POC) testing for infectious diseases. Currently prevailing strategy of developing CRISPR assays exploits only…

. 2023 Apr 23;260:124582. doi: 10.1016/j.talanta.2023.124582. Online ahead of print. Affiliations Expand Affiliations 1 Department of Clinical Laboratory, Chongqing University Three Gorges Hospital, Chongqing, 404000, China. 2 Center for Neuroscience Research, Chongqing Medical University, 400010, Chongqing, China. 3 Department of Clinical Laboratory, Chongqing Wanzhou Shanghai Hospital, Chongqing, 404000, China. 4…

All experimental procedures described in this manuscript were carried out in accordance with relevant guidelines and regulations including biosafety and chemical safety regulations. All nucleic acid sequences used were obtained from publicly available NCBI/GenBank collections and no human subject research was conducted in the course of this project. No materials…

Severe fever with thrombocytopenia syndrome virus (SFTSV) is a re-emerging pathogen causing sporadic outbreaks in China, Japan, and Korea with mortality rates of up to 30%. There are currently no licensed vaccines or therapeutics, and early diagnosis of infected cases is critical for outbreak control and prevention. Assays based on…

Following the first reports of Severe fever with thrombocytopenia syndrome virus (SFTSV) during 2009 in Central China [1], there have been outbreaks reported in over 20 Chinese provinces in subsequent years [2], as well as SFTSV cases reported in Japan [3] and Korea [4]. The early, non-specific clinical symptoms of…

Almazán C, González-Álvarez VH, Fernández de Mera IG, Cabezas-Cruz A, Rodríguez-Martínez R, De la Fuente J (2016) Molecular identification and characterization of Anaplasma platys and Ehrlichia canis in dogs in Mexico. Ticks Tick Borne Dis 7:276–283. doi.org/10.1016/j.ttbdis.2015.11.002 Article  PubMed  Google Scholar  Aman R, Mahas A, Marsic T, Hassan N, Mahfouz…

Bibliographic Details Author Liu, Xin Cui, Johnson Q. Yao, Shuhuai Issue Date 2021 Source MicroTAS 2021 – 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 2021, p. 89-90 Abstract The isothermal amplification coupled with CRISPR detection has attracted tremendous interest for the sensitive, specific, and qualitative detection…

(PDF) Deployable CRISPR-Cas13a diagnostic tools to detect and report Ebola and Lassa virus cases in real-time | John Demby Sandi – Academia.edu Academia.edu uses cookies to personalize content, tailor ads and improve the user experience. By using our site, you agree to our collection of information through the use of…

Levine AJ, Finlay CA, Hinds PW (2004) P53 is a tumor suppressor gene. Cell 116:S67–S69 Article  CAS  PubMed  Google Scholar  Sigal A, Rotter V (2000) Oncogenic mutations of the p53 tumor suppressor: the demons of the guardian of the genome. Can Res 60:6788–6893 CAS  Google Scholar  Sammons MA, Nguyen T-AT,…

Introduction Maize (Zea mays L.) is one of the most important cereal crops in the world. In 2011, a large outbreak of maize lethal necrosis disease (MLND) occurred in the Southern Rift Valley of Kenya (Wangai et al., 2012), caused up to 100% losses in maize yield, and affected the income…

Accurate taxonomic identification is foundational for effective species monitoring and management. When visual identifications are infeasible or inaccurate, genetic approaches provide a reliable alternative. However, these approaches are sometimes less viable (e.g., need for near real-time results, remote locations, funding concerns, molecular inexperience). In these situations, CRISPR-based genetic tools can…

Work by Adler et al.2 describes a 2-step in vitro technique for efficient genome editing of phages involving the broad-spectrum bacterial CRISPR–Cas13a. Cas13 effectors are RNA-guided RNA nucleases with catalytic activity against 2 higher eukaryotic and prokaryotic nucleotide-binding domains. The authors elegantly demonstrated how the Cas13a system from Leptotrichia buccalis…

Infectious diseases, believed to be a thing of the past only a few decades ago owing to the discovery of antibiotics, have resurfaced as an area of active research with the rampant spread of antimicrobial resistance and evolving viruses. Caused by pathogens ranging in size from viruses to worms that…

● Description Recombinant L. buccalis LbuCas13a (type VI-A CRISPR-associated RNA-guided ribonuclease Cas13a), tag free. Cas13a is an RNA-guided endonuclease that belongs to the class 2 type VI CRISPR-Cas system. When activated, Cas13a induces collateral cleavage of nearby non-targeted RNAs in a nonspecific manner. ● Product Data Gallery 4-20% SDS-PAGE Coomassie…

Background & aims: The WHO declared to eliminate hepatitis B virus (HBV) by 2030. However, an increasing number of patients are presenting with low-level viremia (LLV) with the widespread use of antiviral medications. The diagnostic efficiency and coverage area of HBV infection are low. Hence, this study intended to drive…

doi: 10.1021/acssensors.2c01683. Online ahead of print. Affiliations Expand Affiliations 1 School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001, China. 2 Henan Key Laboratory of Targeting Therapy and Diagnosis for Critical Diseases, Zhengzhou 450001, China. 3 School of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, China. 4 State Key Laboratory of…

Introduction Tuberculosis (TB), caused by Mycobacterium tuberculosis (MTB) complex, remains one of the leading infectious causes of death worldwide, with an estimated 1.6 million TB deaths in 2021 (World Health Organization, 2022). Rapid and accurate TB diagnosis is critical to ensure timely initiation of anti-TB therapy (Walzl et al., 2018)….

CRISPR-associated protein Cas9 (white) from Staphylococcus aureus based on Protein Database ID 5AXW. Credit: Thomas Splettstoesser (Wikipedia, CC BY-SA 4.0) Most cancer diagnostic techniques rely on uncomfortable and invasive procedures, such as biopsies, endoscopies or mammograms. Blood samples could be a less unpleasant option, though only a few forms of…

Hampton, H. G., Watson, B. N. J. & Fineran, P. C. The arms race between bacteria and their phage foes. Nature 577, 327–336 (2020). Article  CAS  Google Scholar  Makarova, K. S. et al. Evolution and classification of the CRISPR-Cas systems. Nat. Rev. Microbiol. 9, 467–477 (2011). Article  CAS  Google Scholar …

Integrating artificial intelligence with SARS-CoV-2 diagnostics can help in the timely execution of pandemic control and monitoring plans. To improve the efficiency of the diagnostic process, this study aims to classify fluorescent images via traditional machine learning and deep learning-based transfer learning. A previous study reported a CRISPR-Cas13a system combined…

7.3.1Type VI-A (Cas13a)In Cas13a, direct repeat region of crRNA is composed of 5–6 bp stem using Watsonand Crick base pairing with an unpaired 2 nt bulge (AA or AC) present at 30end ofstem, while loop region of stem contains 7–9 nt depending upon homologs ofCas13a. In addition, an 8–12 nt…

doi: 10.1038/s41589-022-01135-y. Online ahead of print. Jie Yang #  1 , Yang Song #  2   3 , Xiangyu Deng  4 , Jeffrey A Vanegas  1 , Zheng You  1 , Yuxuan Zhang  2   3 , Zhengyan Weng  2   3 , Lori Avery  5 , Kevin D Dieckhaus  6 , Advaith Peddi  4 , Yang Gao  7 , Yi Zhang  8…

Introduction The clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated (Cas) protein systems naturally exist in prokaryotes. They are RNA-mediated defense mechanisms to protect bacteria and archaea from invading nucleic acids (Barrangou et al., 2007). CRISPR–Cas systems are classified into two classes (1 and 2). Class 2 CRISPR–Cas require a single…

journal contribution posted on 19.05.2022, 07:04 by Dalin He, Gang Liu, Jing Yang, Xiaoning Jiang, Hongzhi Wang, Yaru Fan, Shangyu Gong, Feng Wei, Youxiang Diao, Yi Tang In China, drastic losses in the economy have been caused by the Tembusu virus (TMUV), the causative agent of the egg-drop syndrome, to…

Objectives CRISPR-Cas13a system-based nucleic acid detection methods are reported to have rapid and sensitive DNA detection. However, the screening strategy for crRNAs that enables CRISPR-Cas13a single-base resolution DNA detection of human pathogens remains unclear. Methods A combined rational design and target mutation-anchoring CRISPR RNA (crRNA) screening strategy was proposed. Results…

Biogenesis, biology and characterization of circular RNAs Kristensen LS et al. The biogenesis, biology and characterization of circular RNAs. Nat Rev Genet. (2019) Summary text Biogenesis and properties of circRNAs Biogenesis of cirRNA Characteristics of circRNAs Discover and analyze circRNAs circRNA genome-wide analysis CircRNA site-specific analysis circRNA visualization Biological functions…

PDF.MPro – Full Text Journal Article: Exploiting the orthogonal CRISPR-Cas12a/Cas13a trans-cleavage for dual-gene virus detection using a handheld device. Home / Search / Abstract 34653714 (Page 1) Advertisement Find full text journal articles PMID: 34653714 (view PubMed database entry)DOI: 10.1016/j.bios.2021.113701 (read at publisher’s website ) Tian Tian, Zhiqiang Qiu, Yongzhong…

19 Pages Posted: 23 Mar 2022 See all articles by Pengfei Song University of Science and Technology Beijing University of Science and Technology Beijing University of Science and Technology Beijing University of Science and Technology Beijing University of Science and Technology…

This article was originally published here Anal Chim Acta. 2022 May 1;1205:339749. doi: 10.1016/j.aca.2022.339749. Epub 2022 Mar 22. ABSTRACT The COVID-19 pandemic has brought to light the need for fast and sensitive detection methods to prevent the spread of pathogens. The scientific community is making a great effort to design…

The diagnosis and treatment of viral infections, especially those that cause large-scale outbreaks, is urgently needed, as the lack of these measures allows epidemics to progress. A new Biosafety and Health study discusses the use of the highly efficient clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) technology to…

Available online 13 January 2022, 113994 doi.org/10.1016/j.bios.2022.113994Get rights and content Highlights • A one-pot CRISPR/Cas13a-based visual biosensor for the rapid and low-cost nucleic acid detection is proposed. • With a newly developed tube-in-tube vessel used in the biosensor, aerosol cross contamination could be completely avoided. • The sensor can not…