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Tag: CAS13A
Advancements in CRISPR-Based Pathogen Detection: Limitations and Recent Progress
Gene editing technologies, particularly those based on Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR), have revolutionized our ability to accurately and swiftly detect pathogens. However, even these advanced methods have their limitations. This article explores these limitations and highlights recent advancements that are paving the way for faster, more accurate…
Development and evaluation of a CRISPR-Cas13a system-based diagnostic for hepatitis E virus
Objectives: Hepatitis E virus (HEV) is the leading cause of acute viral hepatitis worldwide. HEV RNA detection is the gold standard for HEV infection diagnosis and PCR methods are commonly used but are usually time-consuming and expensive, resulting in low detection efficiency and coverage, especially in low-income areas. Here, we…
Based on CRISPR-Cas13a System, to Establish a Rapid Visual Detection Method for Avian Influenza Viruses
1Hebei Agricultural University, China 2Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, China 3Hebei Sanshi Biotechnology Co., Ltd, China The final, formatted version of the article will be published soon. Notify me Receive an email when it…
Light-Triggered Signal Enhancement Strategy Integrated with a CRISPR/Cas13a-Based Assay for Ultrasensitive and Specific miRNA Detection
The development of facile, accurate, and affordable assays for microRNAs (miRNAs) in early cancer is greatly desirable but encounters an obstacle due to low cellular abundance in biofuids. In this study, we present a novel approach called a light-triggered exponential amplification strategy coupled with a CRISPR/Cas13a-based diagnostic system (LEXPA-CRISPR), which…
Fashion productsAIDS Patient Care and STDs Mary Ann Liebert, Inc., publishers, marital aids meaning
4.9 (264) · $ 1.00 · In stock Description Publications Social Intervention Group STAMP-Based Digital CRISPR-Cas13a for Amplification-Free AIDS Patient Care and STDs Template – Mary Ann Liebert, Inc. PDF) Rehabilitation in HIV/AIDS: Development of an Expanded Mary Ann Liebert, Inc. to publish Journal of PDF)…
In what ways does the CRISPR-Cas13a system enable efficient phage genome editing?
What are the molecular mechanisms of CRISPR-Cas immunity in bacteria?5 answersCRISPR-Cas systems in bacteria provide adaptive and hereditary immunity against foreign nucleic acids. The systems consist of CRISPR arrays containing short DNA fragments called spacers and Cas proteins that facilitate spacer acquisition, processing, and recognition of targeted nucleic acids. The…
Unveiling the RNA-mediated allosteric activation discloses functional hotspots in CRISPR-Cas13a
doi: 10.1093/nar/gkad1127. Online ahead of print. Affiliations Expand Affiliations 1 Department of Bioengineering, University of California Riverside, Riverside, CA , 92521, USA. 2 Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA. 3 Center for RNA Biology, University of Rochester School of Medicine and…
New design strategies for ultra-specific CRISPR-Cas13a-based RNA detection with single-nucleotide mismatch sensitivity
doi: 10.1093/nar/gkad1132. Online ahead of print. Affiliations Expand Affiliations 1 Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, Rochester, NY, USA. 2 Center for RNA Biology, School of Medicine and Dentistry, University of Rochester, Rochester, NY, USA. 3 Department of Biomedical Genetics, School of Medicine…
Upgrading Phage Therapies to Crush Antimicrobial Resistance
By Andy Tay, PhD Overuse of antibiotics has accelerated the emergence of antimicrobial-resistant (AMR) bacterial species. The World Health Organization named antibacterial resistance as a top public health threat to humanity. In 2022, the Antimicrobial Resistance Collaborators reported that AMR bacteria directly caused 1.3 million deaths and was associated with…
Detection of Clostridium perfringens Using Novel Methods Based on Recombinase-Aided Amplification Assay-Assisted CRISPR/Cas12a System
Clostridium perfringens is a highly versatile pathogen of humans and animals. Rapid and sensitive detection methods for C. perfringens are urgently needed for the timely implementation of control. In this study, to provide novel promising methods for the detection of C. perfringens, two rapid, sensitive, and instrument-free C. perfringens detection…
IJMS | Free Full-Text | CRISPR/Cas9 Landscape: Current State and Future Perspectives
1. Introduction Genome editing has taken a leading position among genome modification technologies in a short time and is now widely used in gene therapy. To date, there are three main systems for genome editing: zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and CRISPR/Cas nucleases. Genome editing has…
Rapid in situ RNA imaging based on Cas12a thrusting strand displacement reaction | Nucleic Acids Research
Abstract RNA In situ imaging through DNA self-assembly is advantaged in illustrating its structures and functions with high-resolution, while the limited reaction efficiency and time-consuming operation hinder its clinical application. Here, we first proposed a new strand displacement reaction (SDR) model (Cas12a thrusting SDR, CtSDR), in which Cas12a could overcome…
Rapid and sensitive detection of Pseudomonas aeruginosa by isothermal amplification combined with Cas12a-mediated detection
P. aeruginosa is a common pathogenic bacterium, which seriously threaten human health12,13. In 2017, the World Health Organization (WHO) added P. aeruginosa to the short list of antibiotic-resistant pathogens, the so-called ESKAPE group of pathogens. Traditional pathogenic microbial detection methods require the isolation, culture, and biochemical identification of pathogenic bacteria,…
RPA biosensors for rapid zoonoses screening
Introduction Since the emergence of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) in 2019, this coronavirus has spread to more than 200 countries, cumulatively infecting 672 million people and causing 6.84 million deaths. Moreover, the emergence of numerous variant strains (α, β, γ, δ, and Omicron) presents a serious challenge for…
Multiplex bacteria detection using one-pot CRISPR/Cas13a-based droplet microfluidics
. 2023 Oct 18:243:115771. doi: 10.1016/j.bios.2023.115771. Online ahead of print. Affiliations Expand Affiliations 1 Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing, 100084, PR China. 2 Beijing Key Laboratory of Microanalytical Methods…
Rapid Detection of H5 Subtype Avian Influenza Virus Using CRISPR Cas13a Based-Lateral Flow Dipstick
1China Animal Health and Epidemiology Center, China 2College of Life Sciences and Food Engineering, Hebei University of Engineering, China The final, formatted version of the article will be published soon. Notify me Receive an email when it is updated You just subscribed…
CRISPR/Cas13a-Assisted Accurate and Portable Hepatitis D Virus RNA Detection
Abstract & ims: Hepatitis delta virus (HDV) infection accelerates the progression of chronic hepatitis B virus (HBV) infection, posing a large economic and health burden to patients. At present, there remains a lack of accurate and portable detection methods for HDV RNA. Here, we aim to establish a convenient, rapid,…
Cas13’s Intrinsic Targeting of Host RNA Limits Its Utility
Cas13 is capable of intrinsically targeting host RNA in mammalian cells through mechanisms that were not previously understood. Through transcript-specific mechanisms that are independent of the CRISPR RNA sequence and dynamically dependent on the conformational state of Cas13, Cas13 can also cleave host RNA. This effect is demonstrated by a…
Intrinsic targeting of host RNA by Cas13 constrains its utility
Abudayyeh, O. O. et al. C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector. Science 353, aaf5573 (2016). Article PubMed PubMed Central Google Scholar Terns, M. P. CRISPR-based technologies: impact of RNA-targeting systems. Mol. Cell 72, 404–412 (2018). Article CAS PubMed PubMed Central Google Scholar Abudayyeh, O. O. et al….
Sensitive, rapid detection of NCOA4-m6A towards precisely quantifying radiation dosage on a Cas13a-Microdroplet platform
. 2023 Oct 11:242:115753. doi: 10.1016/j.bios.2023.115753. Online ahead of print. Zhiyuan Jin 1 , Zaizai Dong 2 , Xi Zhao 3 , Xinxin Hang 1 , Yiming Lu 4 , Qi Zhang 5 , Hongxia Chen 3 , Zhaocun Huang 1 , Yusen Wang 1 , Gangqiao Zhou 6 , Lingqian Chang 7 Affiliations Expand Affiliations 1 Beijing Advanced Innovation…
Foods | Free Full-Text | Qualitative and Quantitative Detection of CRISPR-Associated Cas Gene in Gene-Edited Foods
1. Introduction Gene editing technology can effectively edit target genes and is therefore a valuable tool in biological research [1,2,3]. The third-generation gene editing technology, namely clustered regularly interspaced short palindromic repeats (CRISPR), is a defense system in bacteria. The presence of these repeats was first discovered in Escherichia coli…
Atomistic understanding of the RNA-mediated allosteric crosstalk in Cas13a
Cas13a is the one of the latest additions in the CRISPR-Cas library that exclusively targets RNA, a remarkable property being leveraged for the development of tools for RNA detection, regulation and imaging. Cas13a uses a CRISPR RNA (crRNA) sequence as a guide to target RNA sequences, which are then cleaved…
CRISPR-Cas13a-based detection method for avian influenza virus
Yuhan Wu1 Jiaxing Zhan1 Zhaomeng Shan1 Yanbing Li2 Yan Li1 Yixin Wang1 Zhe Liu1 Xuexia Wen1* Xiurong Wang2* 1Shenyang Agricultural University, China 2Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, China The final, formatted version of the article will be published soon. …
Highly sensitive and rapid point-of-care testing for HIV-1 infection based on CRISPR-Cas13a system
Background: Human immunodeficiency virus type one (HIV-1) is the leading cause of acquired immunodeficiency syndrome (AIDS). AIDS remains a global public health concern but can be effectively suppressed by life-long administration of combination antiretroviral therapy. Early detection and diagnosis are two key strategies for the prevention and control of HIV/AIDS….
Highly sensitive and rapid point-of-care testing for HIV-1 infection based on CRISPR-Cas13a system | BMC Infectious Diseases
Selection of RT-RAA amplification primers and HIV-1 RNA nucleic acid target, development of ERASE detection method for HIV-1 RNA In this study, a rapid nucleic acid detection assay for HIV-1 RNA was developed based on the ERASE method for COVID-19 detection established by our collaborator (Fig. 1A). First, HIV-1 RNA extracted…
Target-mediated rolling circle transcription coupling with CRISPR/Cas12a-Cas13a for simultaneous detection of HPV16 and HPV18
Simultaneous detection of multiple targets can provide important data support for clinical diagnosis and treatment. Here, we report a facile isothermal assay based on target-mediated rolling circle transcription coupling with CRISPR/Cas12a-Cas13a (TM-RCT/Cas12a-Cas13a). Through facile one-step amplification (TM-RCT), two target DNAs are converted to RNA amplified products. The simultaneous detection of…
Model-directed generation of CRISPR-Cas13a guide RNAs designs artificial sequences that improve nucleic acid detection
Abstract Generating maximally-fit biological sequences has the potential to transform CRISPR guide RNA design as it has other areas of biomedicine. Here, we introduce model-directed exploration algorithms (MEAs) for designing maximally-fit, artificial CRISPR-Cas13a guides – with multiple mismatches to any natural sequence – that are tailored for desired properties around…
Rapid Detection of blaKPC in Carbapenem-Resistant Enterobacterales Based on CRISPR/Cas13a
Materials and Instruments Clinical Strains and Sputum Samples Collection A total of 117 bacterial strains were isolated and collected from sputum samples of individual patients with suspected intestinal infection treated at our hospital during the first half of 2021. Carbapenemase production was confirmed by the Modified Carbapenem Inactivation Method (mCIM)…
Drug delivery systems for CRISPR-based genome editors
Li, Y., Glass, Z., Huang, M., Chen, Z. Y. & Xu, Q. Ex vivo cell-based CRISPR/Cas9 genome editing for therapeutic applications. Biomaterials 234, 119711 (2020). Article CAS PubMed PubMed Central Google Scholar Dong, W. & Kantor, B. Lentiviral vectors for delivery of gene-editing systems based on CRISPR/Cas: current state and…
Ultra-sensitive electrochemiluminescent biosensor for miRNA based on CRISPR/Cas13a trans-cleavage-triggered hybridization chain reaction and magnetic-assisted enrichment
. 2023 Sep 15;190(10):393. doi: 10.1007/s00604-023-05962-1. Affiliations Expand Affiliations 1 Department of Nephrology, People’s Hospital of Bao’an District, Shenzhen, Guangdong, 518000, People’s Republic of China. 2 Bao’an Shenzhen Clinical Medical School of Guangdong Medical University, Shenzhen, Guangdong, 518000, People’s Republic of China. 3 Ministry of Education Key Laboratory for Analysis…
Healthcare and Nursing Community
CRISPR-based diagnostics offer numerous advantages, shifting clinical testing from lab settings to a patient-centric approach. Yet, until now, tests have only targeted DNA. Here, we share how our lab developed SAHARA, a method for RNA detection. Over the last decade, molecular diagnostics have undergone a transformative revolution with the help…
Precise RNA Editing: Cascade Self-Uncloaking Dual-Prodrug Nanoassemblies Based on CRISPR/Cas13a for Pleiotropic Immunotherapy of PD-L1-Resistant Colorectal Cancer,Advanced Functional Materials
CRISPR/Cas13a is a powerful genome editing system for RNA knockdown that holds enormous potential for cancer treatment by targeting currently undruggable oncogenes or immune checkpoints. However, the precise intratumoral activation of CRISPR/Cas13a to maximize the therapeutic efficiency while guaranteeing biosafety remains a daunting challenge. Here, a cascade self-uncloaking nanoassembly (SRC)…
A new CRISPR/Cas13a-powered catalytic electrochemical biosensor for effective and also extremely delicate RNA diagnostics.
Information on Antigen-specific immunotherapy the water quality of streams enable you to assess the effectiveness of past plans as well as to guide long term ecological guidelines. As a result, the positioning water good quality keeping track of areas (WQMSs) has a huge role inside water smog management. Inside the…
One-tube RPA-CRISPR Cas12a/Cas13a rapid detection of methicillin-resistant Staphylococcus aureus,Analytica Chimica Acta
At present, methicillin-resistant Staphylococcus aureus (MRSA) has caused a serious impact on a global scale. The infection and carrier rate of MRSA in the community is increasing year by year, but there is still no convenient detection system for on-site rapid detection. It is very important to select a rapid…
2.1. Materials and r…
2.1. Materials and reagents All oligonucleotides (Table S1) were synthesized and purified by Sangon Biotech (Shanghai, China). Apurinic/apyrimidinic endonuclease 1 (APE1), T4 DNA ligase, Bst DNA polymerase (large fragment), Exonuclease III (Exo III), Exonuclease I (Exo I), T4 polynucleotide kinase (T4 PNK), Uracil DNA glycosylase (UDG), formamidopyrimidine DNA glycosylase (Fpg),…
Cas13a-based multiplex RNA targeting for potato virus Y
Abudayyeh OO, Gootenberg JS, Konermann S, Joung J, Slaymaker IM, Cox DB, Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov K, Regev A, Lander ES, Koonin EV, Zhang F (2016) C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector. Science 353(6299):aaf5573 Article PubMed PubMed Central Google Scholar Ali Z,…
Cas13d-mediated multiplex RNA targeting confers a broad-spectrum resistance against RNA viruses in potato
Boualem, A., Dogimont, C. & Bendahmane, A. The battle for survival between viruses and their host plants. Curr. Opin. Virol. 17, 32–38 (2016). Article CAS PubMed Google Scholar Rashid, M. O., Wang, Y. & Han, C. G. Molecular detection of potato viruses in Bangladesh and their phylogenetic analysis. Plants 9,…
RNA-mediated Allosteric Activation in CRISPR-Cas13a
Abstract Cas13a is a recent addition to the CRISPR-Cas toolkit that exclusively targets RNA, which makes it a promising tool for RNA detection. The protein uses a CRISPR RNA (crRNA) to target RNA sequences, which are cleaved by a composite active site formed by two Higher Eukaryotes and Prokaryotes Nucleotide…
CRISPR-Cas13a-powered electrochemical biosensor for the detection of the L452R mutation in clinical samples of SARS-CoV-2 variants
作 者:Chen, Zhi Wu, Chenshuo Yuan, Yuxuan Xie, Zhongjian Li, Tianzhong Huang, Hao Li, Shuang Deng, Jiefeng Lin, Huiling Shi, Zhe Li, Chaozhou Hao, Yabin Tang, Yuxuan You, Yuehua Al-Hartomy, Omar A. Wageh, Swelm Al-Sehemi, Abdullah G. Lu, Ruitao Zhang, Ling Lin, Xuechun He, Yaqing Zhao, Guojun Li, Defa Zhang, Han 作者机构:Guangzhou Med Univ Qingyuan Peoples Hosp Affiliated Hosp 6 Qingyuan 511518 Guangdong Peoples R China Shenzhen Univ Inst Microscale Optoelect Coll Phys & Optoelect…
Rapid visual nucleic acid detection of Vibrio alginolyticus by RPA combined with CRISPR/Cas13a
Rapid visual nucleic acid detection of Vibrio alginolyticus by RPA combined with CRISPR/Cas13a Abstract Vibrio alginolyticus ( V. alginolyticus) is a common pathogen that infects humans and animals. In addition to causing serious economic losses in aquaculture, it can also infect humans. The rapid detection of nucleic acids of V….
Development of a Naked Eye CRISPR-Cas12a and -Cas13a Multiplex Point-of-Care Detection of Genetically Modified Swine
journal contribution posted on 2023-07-11, 14:06 authored by Yuan Wang, Lanting Fu, Dagang Tao, Xiaosong Han, Bingrong Xu, Manfei Deng, Sheng Li, Changzhi Zhao, Xinyun Li, Shuhong Zhao, Ping Gong, Yu Yang, Emmanuel Mulaya Khazalwa, Yunlong Ma, Jinxue Ruan, Changchun Li, Shengsong Xie The Rapid Visual CRISPR (RAVI-CRISPR) assay employs…
“Science” sub-journal: New method of CRISPR detection
The gold standard for rapid and specific detection of infectious disease agents is nucleic acid-based assays, such as polymerase chain reaction (PCR), but are costly, instrumentally expensive, and require highly trained personnel. Assays based on the CRISPR-Cas system have recently emerged as an alternative to PCR-based diagnostics. CRISPR detection relies…
Method for jointly detecting microorganisms through combination of nucleic acid isothermal amplification and CRISPR/Cas13a, and application of method – Eureka
[0051] Example 1. Establishment of a system based on RAA isothermal amplification and CRISPR/Cas13a detection of Salmonella [0052] 1. Design and synthesis of RAA primers [0053] 1. Design principles [0054] Different from PCR primers, RAA primers are in the range of 30-35nt in length, and there is no palindromic sequence,…
Ultrasensitive Turn-On Detection of Biomarker FEN1 Using a CRISPR/Cas13a System Integrated with a Cleavage-Ligation-Activation Cascade,Sensors and Actuators B: Chemical
Flap endonuclease I (FEN1) is a structure-specific nuclease that participates in DNA repair, and its overexpression is related to an increased risk of cancer. Currently, few FEN1 assays can meet the requirements for diagnostic purposes due to their high complexity, low specificity, and limited sensitivity. Herein, we developed a biosensing…
Ultrasensitive Turn-On Detection of Biomarker FEN1 Using a CRISPR/Cas13a System Integrated with a Cleavage-Ligation-Activation Cascade
Flap endonuclease I (FEN1) is a structure-specific enzyme encoded by the FEN1 gene that is responsible for removing 5′ overhang DNA from branched DNA in mammalian cells [1], [2]. FEN1 has been shown to play a critical role in microhomology-mediated end joining (MMEJ), an error-prone DNA repair pathway [3]. Overexpression…
Ablation of OCT4 function in cattle embryos by double electroporation of CRISPR-Cas for DNA and RNA targeting (CRISPR-DART)
Abstract CRISPR-Cas ribonucleoproteins are important tools for gene editing in pre-implantation embryos. However, the inefficient production of biallelic deletions in cattle zygotes has hindered mechanistic studies of gene function. In addition, the presence of maternal RNAs that support embryo development until embryonic genome activation may cause confounding phenotypes. Here, we…
Lack of Cas13a inhibition by anti-CRISPR proteins from Leptotrichia prophages.
Abstract CRISPR systems are prokaryotic adaptive immune systems that use RNA-guided Cas nucleases to recognize and destroy foreign genetic elements. To overcome CRISPR immunity, bacteriophages have evolved diverse families of anti-CRISPR proteins (Acrs). Recently, Lin et al. (2020) described the discovery and characterization of 7 Acr families (AcrVIA1-7) that inhibit type…
CRISPR/Cas13a catalyzed self-assembly of quantum dot-DNA hydrogel for microRNA assay,Sensors and Actuators B: Chemical
Taking the advantages of outstanding precision in target recognition and trans-cleavage ability, the RNA-guided CRISPR-Cas systems have shown great promise in cancer diagnostics. Here, we developed a novel detection system based on CRISPR-Cas-catalyzed formation of quantum dot-DNA (QD-DNA) hydrogel for sensitive miRNA assay. After target miRNA recognition and Cas-mediated cleavage…
A self-supply crRNA-mediated CRISPR/Cas12a-driven controlled-release homogeneous biosensor for ultrasensitive detection of microRNA
Abnormal expression of microRNAs (miRNAs) will lead to various diseases [1], [2], [3], [4], [5], including cancer, therefore, the ultrasensitive detection of miRNAs is critical for the early cancer diagnosis and therapy. Recently, some analytical methods, such as electrochemistry, Raman, and fluorescence have been widely used to detect miRNAs [6],…
Exosomal circRNA: emerging insights into cancer progression and clinical application potential | Journal of Hematology & Oncology
He G, Peng X, Wei S, Yang S, Li X, Huang M, et al. Exosomes in the hypoxic TME: from release, uptake and biofunctions to clinical applications. Mol Cancer. 2022;21:19. Article PubMed PubMed Central Google Scholar Downs-Canner SM, Meier J, Vincent BG, Serody JS. B cell function in the tumor…
Electrochemical biosensor strategy combining DNA entropy-driven technology to activate CRISPR-Cas13a activity and triple-stranded nucleic acids to detect SARS-CoV-2 RdRp gene
Zhu JJ (2021) Special topic: biomedical application of DNA-assembled nanostructure. J Anal Test 5(2):93–94 Article Google Scholar Pan J, He Y, Liu Z, Chen J (2022) Tetrahedron-based constitutional dynamic network for COVID-19 or other coronaviruses diagnostics and its logic gate applications. Anal Chem 94(2):714–722 Article CAS PubMed Google Scholar Zhang…
CRISPR/Cas13a-Based MicroRNA Detection in Tumor-Derived Extracellular Vesicles
doi: 10.1002/advs.202301766. Online ahead of print. Affiliations Expand Affiliations 1 Center for Systems Biology, Massachusetts General Hospital, Boston, MA, 02114, USA. 2 Cancer Center, Massachusetts General Hospital, Boston, MA, 02114, USA. 3 Department of Radiology, Massachusetts General Hospital, Boston, MA, 02114, USA. Item in Clipboard Jae-Sang Hong et al. Adv Sci…
Sci-Hub | Deployable CRISPR-Cas13a diagnostic tools to detect and report Ebola and Lassa virus cases in real-time. Nature Communications, 11(1)
Sci-Hub | Deployable CRISPR-Cas13a diagnostic tools to detect and report Ebola and Lassa virus cases in real-time. Nature Communications, 11(1) | 10.1038/s41467-020-17994-9 ◂ ↓ save Barnes, K. G., Lachenauer, A. E., Nitido, A., Siddiqui, S., Gross, R., Beitzel, B., … Sabeti, P. C. (2020). Deployable CRISPR-Cas13a diagnostic tools to detect…
LwaCas13a (C2c2) Nuclease – Amerigo Scientific
LwaCas13a (previously known as LwaC2c2) is a crRNA-mediated RNA endonuclease derived from Leptotrichia wadeim. It can specifically recognize and cleave the RNA target with the PFS sequence. In addition, Cas13a also has the trans-cleavage activity, that is, when LwaCas13a binds crRNA and RNA target to form a ternary complex,…
Design of crRNA to Regulate MicroRNAs Related to Metastasis in Colorectal Cancer Using CRISPR-C2c2 (Cas13a) Technique
Colorectal cancer (CRC) is the third most prevalent cancer with the second-highest mortality rate worldwide. microRNAs (miRNAs) of cancer-derived exosomes have shown promising diagnosis potential. Recent studies have shown the metastatic potential of a specific group of microRNAs called metastasis. Therefore, down-regulation of miRNAs at the transcriptional level can reduce…
CRISPR/Cas12a-drived fluorescent and electrochemical signal-off/on dual-mode biosensors for ultrasensitive detection of EGFR 19del mutation
The epidermal growth factor receptor (EGFR), a member of the epidermal growth factor receptor (HER) family, is closely related to the activation of cell signaling pathways, including cell proliferation and apoptosis protection [1], [2]. Studies have been proved that EGFR is over-expressed in various tumors, especially in lung adenocarcinoma. This…
Lack of Cas13a inhibition by anti-CRISPR proteins from Leptotrichia prophages
Summary CRISPR systems are prokaryotic adaptive immune systems that use RNA-guided Cas nucleases to recognize and destroy foreign genetic elements. To overcome CRISPR immunity, bacteriophages have evolved diverse families of anti-CRISPR proteins (Acrs). Recently, Lin et al. (2020) described the discovery and characterization of 7 Acr families (AcrVIA1–7) that inhibit…
RPA-CRISPR/Cas12a mediated isothermal amplification for visual detection of Phytophthora sojae
Introduction Phytophthora sojae is among the most destructive pathogens of soybean (Glycine max). An estimated annual worldwide loss of US$1–2 billion is caused by P. sojae, which was first identified as a soybean pathogen in the 1950s in Indiana, Ohio, and North Carolina (Schmitthenner, 1985; Tyler, 2007; Dorrance, 2018). The…
Multi-faceted CRISPR/Cas technological innovation aspects in the framework of 3P medicine
Golubnitschaja O, Baban B, Boniolo G, Wang W, Bubnov R, Kapalla M, et al. Medicine in the early twenty-first century: paradigm and anticipation – EPMA position paper 2016. EPMA J. 2016;7:23. Article PubMed PubMed Central Google Scholar Kropp M, Golubnitschaja O, Mazurakova A, Koklesova L, Sargheini N, Vo T-TKS, et…
Fighting oral bacteria is elementary with SHERLOCK-based detection tool
Thanks to a novel tool that detects bacteria in saliva using SHERLOCK, the evolution of CRISPR, you may soon be able to leave the dentist after a checkup with more comprehensive information about the health of your mouth. The accurate tool is much faster than existing methods and may lead…
Rapid detection of Nipah virus using the one-pot RPA-CRISPR/Cas13a assay
doi: 10.1016/j.virusres.2023.199130. Online ahead of print. Affiliations Expand Affiliations 1 Viral Hemorrhagic Fevers Research Unit, CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, 200031, China; University of Chinese Academy of Sciences, Beijing, 100049, China. 2 Institute of Pathogenic Microorganisms, Guangdong Provincial…
Potential of CRISPR/Cas system as emerging tools in the detection of viral hepatitis infection | Virology Journal
Tapper EB, Curry MP. Hepatitis Caused by Other Viruses Handbook of Liver Disease, 2018: p. 78. Sarin SK, Kumar M. Viral hepatitis A, in Molecular Pathology of Liver Diseases. Springer; 2011. pp. 527–52. Qu B, Brown RJ. Strategies to inhibit Hepatitis B Virus at the transcript level. Viruses. 2021;13(7):1327. Article CAS …
CRISPR-Cas13a-powered electrochemical biosensor for the detection of the L452R mutation in clinical samples of SARS-CoV-2 variants | Journal of Nanobiotechnology
Liang Y, Lin H, Zou L, Deng X, Tang S. Biosens Bioelectron. 2022;205:114098. Article CAS PubMed PubMed Central Google Scholar Telenti A, Hodcroft EB, Robertson DL. Cold Spring Harb Perspect Med. 2022;12:a041390. Article CAS PubMed Google Scholar Shrestha LB, Foster C, Rawlinson W, Tedla N, Bull RA. Reviews in Medical…
Solid-Phase Extraction and Enhanced Amplification-Free Detection of Pathogens Integrated by Dual-Functional CRISPR-Cas12a
Abstract Public healthcare demands effective and pragmatic diagnostic tools to address the escalating challenges in infection management in resource-limited areas. Recent advance in CRISPR-based biosensing promises the development of next-generation tools for disease diagnostics, including point-of-care (POC) testing for infectious diseases. Currently prevailing strategy of developing CRISPR assays exploits only…
CRISPR/Cas13a-triggered Cas12a biosensing method for ultrasensitive and specific miRNA detection
. 2023 Apr 23;260:124582. doi: 10.1016/j.talanta.2023.124582. Online ahead of print. Affiliations Expand Affiliations 1 Department of Clinical Laboratory, Chongqing University Three Gorges Hospital, Chongqing, 404000, China. 2 Center for Neuroscience Research, Chongqing Medical University, 400010, Chongqing, China. 3 Department of Clinical Laboratory, Chongqing Wanzhou Shanghai Hospital, Chongqing, 404000, China. 4…
Machine learning for design of degenerate Cas13a crRNAs using lassa virus as a model of highly variable RNA target
All experimental procedures described in this manuscript were carried out in accordance with relevant guidelines and regulations including biosafety and chemical safety regulations. All nucleic acid sequences used were obtained from publicly available NCBI/GenBank collections and no human subject research was conducted in the course of this project. No materials…
Development and characterization of a digital CRISPR/Cas13a based assay for rapid and sensitive diagnosis of Severe Fever with Thrombocytopenia Syndrome Virus,Sensors and Actuators B: Chemical
Severe fever with thrombocytopenia syndrome virus (SFTSV) is a re-emerging pathogen causing sporadic outbreaks in China, Japan, and Korea with mortality rates of up to 30%. There are currently no licensed vaccines or therapeutics, and early diagnosis of infected cases is critical for outbreak control and prevention. Assays based on…
Development and characterization of a digital CRISPR/Cas13a based assay for rapid and sensitive diagnosis of Severe Fever with Thrombocytopenia Syndrome Virus
Following the first reports of Severe fever with thrombocytopenia syndrome virus (SFTSV) during 2009 in Central China [1], there have been outbreaks reported in over 20 Chinese provinces in subsequent years [2], as well as SFTSV cases reported in Japan [3] and Korea [4]. The early, non-specific clinical symptoms of…
RPA/CRISPR-cas12a as a specific, sensitive and rapid method for diagnosing Ehrlichia canis and Anaplasma platys in dogs in Thailand
Almazán C, González-Álvarez VH, Fernández de Mera IG, Cabezas-Cruz A, Rodríguez-Martínez R, De la Fuente J (2016) Molecular identification and characterization of Anaplasma platys and Ehrlichia canis in dogs in Mexico. Ticks Tick Borne Dis 7:276–283. doi.org/10.1016/j.ttbdis.2015.11.002 Article PubMed Google Scholar Aman R, Mahas A, Marsic T, Hassan N, Mahfouz…
Droplet Digital Quantification of Nucleic Acids with Cas13a – HKUST SPD
Bibliographic Details Author Liu, Xin Cui, Johnson Q. Yao, Shuhuai Issue Date 2021 Source MicroTAS 2021 – 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 2021, p. 89-90 Abstract The isothermal amplification coupled with CRISPR detection has attracted tremendous interest for the sensitive, specific, and qualitative detection…
(PDF) Deployable CRISPR-Cas13a diagnostic tools to detect and report Ebola and Lassa virus cases in real-time | John Demby Sandi
(PDF) Deployable CRISPR-Cas13a diagnostic tools to detect and report Ebola and Lassa virus cases in real-time | John Demby Sandi – Academia.edu Academia.edu uses cookies to personalize content, tailor ads and improve the user experience. By using our site, you agree to our collection of information through the use of…
Electrochemical detection of the p53 gene using exponential amplification reaction (EXPAR) and CRISPR/Cas12a reactions
Levine AJ, Finlay CA, Hinds PW (2004) P53 is a tumor suppressor gene. Cell 116:S67–S69 Article CAS PubMed Google Scholar Sigal A, Rotter V (2000) Oncogenic mutations of the p53 tumor suppressor: the demons of the guardian of the genome. Can Res 60:6788–6893 CAS Google Scholar Sammons MA, Nguyen T-AT,…
Portable rapid detection of maize chlorotic mottle virus using RT-RAA/CRISPR-Cas12a based lateral flow assay
Introduction Maize (Zea mays L.) is one of the most important cereal crops in the world. In 2011, a large outbreak of maize lethal necrosis disease (MLND) occurred in the Southern Rift Valley of Kenya (Wangai et al., 2012), caused up to 100% losses in maize yield, and affected the income…
Rapid CRISPR-Cas13a genetic identification enables new opportunities for listed Chinook salmon management
Accurate taxonomic identification is foundational for effective species monitoring and management. When visual identifications are infeasible or inaccurate, genetic approaches provide a reliable alternative. However, these approaches are sometimes less viable (e.g., need for near real-time results, remote locations, funding concerns, molecular inexperience). In these situations, CRISPR-based genetic tools can…
Bacteriophage discovery to advance biotechnology and biotherapeutics
Work by Adler et al.2 describes a 2-step in vitro technique for efficient genome editing of phages involving the broad-spectrum bacterial CRISPR–Cas13a. Cas13 effectors are RNA-guided RNA nucleases with catalytic activity against 2 higher eukaryotic and prokaryotic nucleotide-binding domains. The authors elegantly demonstrated how the Cas13a system from Leptotrichia buccalis…
Technologies Spearheading Infectious Disease Research
Infectious diseases, believed to be a thing of the past only a few decades ago owing to the discovery of antibiotics, have resurfaced as an area of active research with the rampant spread of antimicrobial resistance and evolving viruses. Caused by pathogens ranging in size from viruses to worms that…
Cas13a (L. buccalis) Recombinant
● Description Recombinant L. buccalis LbuCas13a (type VI-A CRISPR-associated RNA-guided ribonuclease Cas13a), tag free. Cas13a is an RNA-guided endonuclease that belongs to the class 2 type VI CRISPR-Cas system. When activated, Cas13a induces collateral cleavage of nearby non-targeted RNAs in a nonspecific manner. ● Product Data Gallery 4-20% SDS-PAGE Coomassie…
CRISPR/Cas13a-assisted Rapid and Portable HBV DNA Detection for Low-Level Viremia Patients
Background & aims: The WHO declared to eliminate hepatitis B virus (HBV) by 2030. However, an increasing number of patients are presenting with low-level viremia (LLV) with the widespread use of antiviral medications. The diagnostic efficiency and coverage area of HBV infection are low. Hence, this study intended to drive…
Highly Effective Detection of Exosomal miRNAs in Plasma Using Liposome-Mediated Transfection CRISPR/Cas13a
doi: 10.1021/acssensors.2c01683. Online ahead of print. Affiliations Expand Affiliations 1 School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001, China. 2 Henan Key Laboratory of Targeting Therapy and Diagnosis for Critical Diseases, Zhengzhou 450001, China. 3 School of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, China. 4 State Key Laboratory of…
Development and clinical evaluation of a CRISPR/Cas13a-based diagnostic test to detect Mycobacterium tuberculosis in clinical specimens
Introduction Tuberculosis (TB), caused by Mycobacterium tuberculosis (MTB) complex, remains one of the leading infectious causes of death worldwide, with an estimated 1.6 million TB deaths in 2021 (World Health Organization, 2022). Rapid and accurate TB diagnosis is critical to ensure timely initiation of anti-TB therapy (Walzl et al., 2018)….
Using CRISPR to detect cancer biomarkers
CRISPR-associated protein Cas9 (white) from Staphylococcus aureus based on Protein Database ID 5AXW. Credit: Thomas Splettstoesser (Wikipedia, CC BY-SA 4.0) Most cancer diagnostic techniques rely on uncomfortable and invasive procedures, such as biopsies, endoscopies or mammograms. Blood samples could be a less unpleasant option, though only a few forms of…
Cryo-EM structure and protease activity of the type III-E CRISPR-Cas effector
Hampton, H. G., Watson, B. N. J. & Fineran, P. C. The arms race between bacteria and their phage foes. Nature 577, 327–336 (2020). Article CAS Google Scholar Makarova, K. S. et al. Evolution and classification of the CRISPR-Cas systems. Nat. Rev. Microbiol. 9, 467–477 (2011). Article CAS Google Scholar …
DataSheet1_AI-boosted CRISPR-Cas13a and total internal reflection fluorescence microscopy system for SARS-CoV-2 detection.docx
Integrating artificial intelligence with SARS-CoV-2 diagnostics can help in the timely execution of pandemic control and monitoring plans. To improve the efficiency of the diagnostic process, this study aims to classify fluorescent images via traditional machine learning and deep learning-based transfer learning. A previous study reported a CRISPR-Cas13a system combined…
731 Type VI A Cas13a In Cas13a direct repeat region of crRNA is composed of 5 6
7.3.1Type VI-A (Cas13a)In Cas13a, direct repeat region of crRNA is composed of 5–6 bp stem using Watsonand Crick base pairing with an unpaired 2 nt bulge (AA or AC) present at 30end ofstem, while loop region of stem contains 7–9 nt depending upon homologs ofCas13a. In addition, an 8–12 nt…
Engineered LwaCas13a with enhanced collateral activity for nucleic acid detection
doi: 10.1038/s41589-022-01135-y. Online ahead of print. Jie Yang # 1 , Yang Song # 2 3 , Xiangyu Deng 4 , Jeffrey A Vanegas 1 , Zheng You 1 , Yuxuan Zhang 2 3 , Zhengyan Weng 2 3 , Lori Avery 5 , Kevin D Dieckhaus 6 , Advaith Peddi 4 , Yang Gao 7 , Yi Zhang 8…
Frontiers | A Mutated Nme1Cas9 Is a Functional Alternative RNase to Both LwaCas13a and RfxCas13d in the Yeast S. cerevisiae
Introduction The clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated (Cas) protein systems naturally exist in prokaryotes. They are RNA-mediated defense mechanisms to protect bacteria and archaea from invading nucleic acids (Barrangou et al., 2007). CRISPR–Cas systems are classified into two classes (1 and 2). Class 2 CRISPR–Cas require a single…
Specific High-Sensitivity Enzymatic Molecular Detection System Termed RPA-Based CRISPR-Cas13a for Duck Tembusu Virus Diagnostics
journal contribution posted on 19.05.2022, 07:04 by Dalin He, Gang Liu, Jing Yang, Xiaoning Jiang, Hongzhi Wang, Yaru Fan, Shangyu Gong, Feng Wei, Youxiang Diao, Yi Tang In China, drastic losses in the economy have been caused by the Tembusu virus (TMUV), the causative agent of the egg-drop syndrome, to…
A Highly Sensitive and Specific Detection Method for Mycobacterium tuberculosis Fluoroquinolone Resistance Mutations Utilizing the CRISPR-Cas13a System
Objectives CRISPR-Cas13a system-based nucleic acid detection methods are reported to have rapid and sensitive DNA detection. However, the screening strategy for crRNAs that enables CRISPR-Cas13a single-base resolution DNA detection of human pathogens remains unclear. Methods A combined rational design and target mutation-anchoring CRISPR RNA (crRNA) screening strategy was proposed. Results…
Biogenesis, biology and characterization of circular RNAs
Biogenesis, biology and characterization of circular RNAs Kristensen LS et al. The biogenesis, biology and characterization of circular RNAs. Nat Rev Genet. (2019) Summary text Biogenesis and properties of circRNAs Biogenesis of cirRNA Characteristics of circRNAs Discover and analyze circRNAs circRNA genome-wide analysis CircRNA site-specific analysis circRNA visualization Biological functions…
Full Text Journal Article: Exploiting the orthogonal CRISPR-Cas12a/Cas13a trans-cleavage for dual-gene virus detection using a handheld device.
PDF.MPro – Full Text Journal Article: Exploiting the orthogonal CRISPR-Cas12a/Cas13a trans-cleavage for dual-gene virus detection using a handheld device. Home / Search / Abstract 34653714 (Page 1) Advertisement Find full text journal articles PMID: 34653714 (view PubMed database entry)DOI: 10.1016/j.bios.2021.113701 (read at publisher’s website ) Tian Tian, Zhiqiang Qiu, Yongzhong…
Crispr/Cas13a Induced Exponential Amplification for Highly Sensitive and Specific Detection of Circular Rna by Pengfei Song, Pengbo Zhang, Ke Qin, Fengxia Su, Kejian Gao, Xin Liu, Zhengping Li :: SSRN
19 Pages Posted: 23 Mar 2022 See all articles by Pengfei Song University of Science and Technology Beijing University of Science and Technology Beijing University of Science and Technology Beijing University of Science and Technology Beijing University of Science and Technology…
CASCADE: Naked eye-detection of SARS-CoV-2 using Cas13a and gold nanoparticles
This article was originally published here Anal Chim Acta. 2022 May 1;1205:339749. doi: 10.1016/j.aca.2022.339749. Epub 2022 Mar 22. ABSTRACT The COVID-19 pandemic has brought to light the need for fast and sensitive detection methods to prevent the spread of pathogens. The scientific community is making a great effort to design…
Using CRISPR/Cas to develop biosafety materials
The diagnosis and treatment of viral infections, especially those that cause large-scale outbreaks, is urgently needed, as the lack of these measures allows epidemics to progress. A new Biosafety and Health study discusses the use of the highly efficient clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) technology to…
A one-pot CRISPR/Cas13a-based contamination-free biosensor for low-cost and rapid nucleic acid diagnostics
Available online 13 January 2022, 113994 doi.org/10.1016/j.bios.2022.113994Get rights and content Highlights • A one-pot CRISPR/Cas13a-based visual biosensor for the rapid and low-cost nucleic acid detection is proposed. • With a newly developed tube-in-tube vessel used in the biosensor, aerosol cross contamination could be completely avoided. • The sensor can not…