Tag: cas9

Biotech startup Helex brings precision to gene editing

From Jurassic Park to Elysium, science fiction movies have used genetic engineering as a plot device to show the dark side of technology-led intervention in natural processes. In the scientific community, however, genetic editing is looked upon as a potential way to eradicate diseases in plants and animals–including humans. CRISPR…

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Beam Therapeutics Stock: A Leader in Gene Editing Tech?

Don’t invest in stories. Don’t invest in a company before they have meaningful revenues. Don’t invest in drug developers that have a great deal of regulatory risk. These are all rules we need to break if we want exposure to a technology that allows mankind to start playing with the…

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CRISPR-Cas technology a new era in genomic engineering

Review doi: 10.1016/j.btre.2022.e00731. eCollection 2022 Jun. Affiliations Expand Affiliation 1 Department of Agriculture and Natural Resources, College of Agriculture, Science, and Technology, Delaware State University, Dover, DE 19901, United States of America. Free PMC article Item in Clipboard Review Ali Parsaeimehr et al. Biotechnol Rep (Amst). 2022. Free PMC article Show…

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A desirable transgenic strategy using GGTA1 endogenous promoter-mediated knock-in for xenotransplantation model

Lai, L. et al. Production of alpha-1,3-galactosyltransferase knockout pigs by nuclear transfer cloning. Science 295, 1089–1092. doi.org/10.1126/science.1068228 (2002). ADS  CAS  Article  PubMed  Google Scholar  Kuwaki, K. et al. Heart transplantation in baboons using alpha1,3-galactosyltransferase gene-knockout pigs as donors: initial experience. Nat. Med. 11, 29–31. doi.org/10.1038/nm1171 (2005). CAS  Article  PubMed  Google…

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New CRISPR-Based Map Opens Door to New Genetic Testing, Therapeutic Possibilities

Researchers from all around the world have been working on mapping human genes to their functions. Now, for the first time, that mapping has been completed, and it opens the doors for all kinds of genetic testing and medicinal possibilities. In 2003, members of the Human Genome Project completely sequenced…

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Cas12c provides antiviral immunity without the need to cut DNA

Graphical abstract. Credit: Molecular Cell (2022). DOI: 10.1016/j.molcel.2022.04.020 A trio of researchers at the University of California, Berkeley, has found that the protein Cas12c can provide antiviral immunity for bacteria without the need to cut DNA. In their paper published in the journal Molecular Cell, Carolyn Huang, Benjamin Adler and…

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Immatics and Editas Medicine Enter Strategic Research Collaboration and Licensing Agreement to Combine Gamma-Delta T Cell Adoptive Cell Therapies and Gene Editing for the Treatment of Cancer

Get instant alerts when news breaks on your stocks. Claim your 1-week free trial to StreetInsider Premium here. TUEBINGEN, Germany and HOUSTON and CAMBRIDGE, Mass., June 07, 2022 (GLOBE NEWSWIRE) — Immatics N.V. (Nasdaq: IMTX, “Immatics”), a clinical-stage biopharmaceutical company active in the discovery and development of T cell-redirecting cancer…

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New gene-edited tomatoes boost vitamin D levels

An international team of scientists primarily based out of the John Innes Centre in the United Kingdom has used gene-editing to create a vitamin D-enriched tomato. Using CRISPR-Cas9, they switched off an enzyme in tomatoes that ordinarily prevents the molecular forerunner of vitamin D3 (called 7-dehydrocholesterol, or 7-DHC) from accumulating…

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Bio-informatic analysis of CRISPR protospacer adjacent motifs (PAMs) in T4 genome | BMC Genomic Data

The clustered regularly interspaced short palindromic repeats-Cas (CRISPR-Cas) defensive system is an acquired mechanism in prokaryotes which is analogous to RNAi in eukaryotes [1]. The CRISPR machinery is a complex immune strategy that is continually evolving in the bacterial genome to accommodate the rapid changes in the nucleic acids of…

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Frontiers | A Mutated Nme1Cas9 Is a Functional Alternative RNase to Both LwaCas13a and RfxCas13d in the Yeast S. cerevisiae

Introduction The clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated (Cas) protein systems naturally exist in prokaryotes. They are RNA-mediated defense mechanisms to protect bacteria and archaea from invading nucleic acids (Barrangou et al., 2007). CRISPR–Cas systems are classified into two classes (1 and 2). Class 2 CRISPR–Cas require a single…

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Convenience-sized RNA editing | MIT News

Last year, researchers at MIT’s McGovern Institute for Brain Research discovered and characterized Cas7-11, the first CRISPR enzyme capable of making precise, guided cuts to strands of RNA without harming cells in the process. Now, working with collaborators at the University of Tokyo, the same team has revealed that Cas7-11…

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Turn Your Hamster Into a Killer With This One Simple Step – CRISPR Gene Editing

After carrying out a gene-editing trial on hamsters, a group of neuroscience experts were left “very startled” by the outcome, which showed that the normally placid rodents had been transformed into “aggressive” creatures. According to the press statement, the researchers utilized Syrian hamsters and CRISPR-Cas9, a cutting-edge tech that provides…

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Nature Papers on Genome of Individual From Pompeii, CircRNA Study Best Practices, More

The first sequenced genome of a person who died in Pompeii from the eruption of Mount Vesuvius is published in Scientific Reports this week. Pompeii, which was a major Roman port city in Italy, has been a major archeological site due to the well-preserved nature of both buildings and human…

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Scientists Genetically Edited Regular Tomatoes To Improve Vitamin D Levels

Scientists have genetically edited tomatoes that are more nutritious in provitamin D3 — a precursor to vitamin D, and they’re as high as two whole eggs or a tablespoon full of tuna. Unsplash Also Read: Tomato Maestros At Heinz Mimicked Mars To Create Alien Ketchup They did so by disabling a…

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Researchers Develop Allogeneic CART for Relapsed or Refractory T-ALL Using Base Editing

A group of researchers at Children’s Hospital of Philadelphia (CHOP), in collaboration with Beam Therapeutics, have tested and developed an “off-the-shelf” chimeric antigen receptor T-cell (CART) using base editing, which is designed to allow for precise editing of the CART with less risk of unwanted and unforeseen outcomes that may…

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Researchers Develop New Mechanism with CRISPR Technology to Combat Polygenic Diseases

Rise in Black Fungus infection hints at the necessity to monitor and control diabetes Researchers from Rice University, Houston, have developed a new mechanism that allows the editing of multiple DNA sites all at once, with the help of CRISPR/Cas9 technology. This new mechanism, referred to as the ‘drive-and-process’ or…

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CRISPR Therapeutics AG (NASDAQ:CRSP) Expected to Announce Earnings of -$2.23 Per Share

Equities analysts predict that CRISPR Therapeutics AG (NASDAQ:CRSP – Get Rating) will post ($2.23) earnings per share for the current quarter, Zacks reports. Six analysts have made estimates for CRISPR Therapeutics’ earnings. The highest EPS estimate is ($1.92) and the lowest is ($2.48). CRISPR Therapeutics posted earnings of $9.44 per…

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WGS Facilitates Gene Editing System Upgrade

Researchers at the Korean Institute of Life Sciences and Technology engineered an efficient, miniaturized CRISPR-Cas gene-editing system that may be more easily packed into vectors for clinical applications. Their system employs the Cas variant Cas12f1 with a guide RNA (gRNA) remodeled to mitigate off-target effects, a design that could potentially…

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Novel supramolecular CRISPR-Cas9 carrier enables more efficient genome editing — ScienceDaily

Clustered regularly interspaced short palindromic repeats (CRISPR) and their accompanying protein, CRISPR-associated protein 9 (Cas9), made international headlines a few years ago as a game-changing genome editing system. Consisting of Cas9 and strand of genetic material known as a single-guide RNA (sgRNA), the system can target specific regions of DNA…

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Editas Medicine (EDIT) Falls 4.05% on May 10

Last Price $ Last Trade Change $ Change Percent % Open $ Prev Close $ High $ low $ 52 Week High $ 52 Week Low $ Market Cap PE Ratio Volume Exchange EDIT – Market Data & News Trade Editas Medicine Inc (NASDAQ: EDIT) has fallen $0.45 (4.05%) and …

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Global CRISPR/Cas9 Market In- Depth Research, Industry Statistics 2022

Global “CRISPR/Cas9 Market” Reports 2022 provide key industry studies for CRISPR/Cas9 manufacturers with specific statistics, significance, definition, SWOT analysis, expert opinion and the latest developments in the world. The research report also covers market size, price, sales, revenue, market shares, gross margin, growth rate and cost structure. The report aims…

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Intellia (NTLA) Drops 4.76% on May 5

Last Price $ Last Trade Change $ Change Percent % Open $ Prev Close $ High $ low $ 52 Week High $ 52 Week Low $ Market Cap PE Ratio Volume Exchange NTLA – Market Data & News Trade Intellia Therapeutics Inc (NASDAQ: NTLA) shares are down 4.76%, or $2.65 per…

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ISPE announces 2022 Facility of the Year Awards winners

Winners of ISPE’s Facility of the Year Awards (FOYA) 2022 include Janssen, Takeda, CRISPR Therapeutics, Catalent and Iovance Biotherapeutics. ISPE has announced the 2022 Facility of the Year Awards (FOYA) Category Winners at the 2022 ISPE Europe Annual Conference in Madrid, Spain.  FOYA is the global awards programme recognising innovation and creativity…

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Identifying Mechanisms of Methylation in Stem Cells

Normal cells (red) survive much longer than Set1A-mutant cells (green) when ING5 is deleted. Northwestern Medicine scientists have identified critical regulatory processes that govern differentiation in embryonic stem cells, according to a study published in the Proceedings of the National Academy of the Sciences (PNAS). Bercin Cenik, MD, PhD, postdoctoral…

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New Products Posted to GenomeWeb: Thermo Fisher, Guardant Health, EmpowerDx, More

Thermo Fisher Invitrogen TrueTag Donor DNA Kits  Thermo Fisher Scientific has launched Invitrogen TrueTag Donor DNA kits for cell line engineering with CRISPR-Cas9 or TALEN technology. They allow researchers to enrich tagged cells and skip clonal isolation. The kits support four types of locus-specific knock-in templates and feature a one-step…

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Technical Officer / Micro-injectionist job with EMBL

The European Molecular Biology Laboratory (EMBL) is one of the highest-ranked scientific research organisations in the world. The Headquarters Laboratory is located in Heidelberg (Germany), with additional sites in Barcelona (Spain), Grenoble (France), Hamburg (Germany), Hinxton (UK), and Rome (Italy). The EMBL Epigenetics and Neurobiology Unit in Rome is supported…

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Recent developments in miRNA based recombinant protein expression in CHO

Aguiar TQ, Santos SB, Martins IM, Domingues L, Oliveira C (2019) Production and bioengineering of recombinant pharmaceuticals. Proteins: sustainable source, processing and applications. Elsevier, Amsterdam, pp 259–293 Chapter  Google Scholar  Amadi IM, Agrawal V, Christianson T, Bardliving C, Shamlou P, LeBowitz JH (2020) Inhibition of endogenous miR-23a/miR-377 in CHO cells…

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Biogenesis, biology and characterization of circular RNAs

Biogenesis, biology and characterization of circular RNAs Kristensen LS et al. The biogenesis, biology and characterization of circular RNAs. Nat Rev Genet. (2019) Summary text Biogenesis and properties of circRNAs Biogenesis of cirRNA Characteristics of circRNAs Discover and analyze circRNAs circRNA genome-wide analysis CircRNA site-specific analysis circRNA visualization Biological functions…

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Multiplexed genome regulation in vivo with hyper-efficient Cas12a

. 2022 Apr;24(4):590-600. doi: 10.1038/s41556-022-00870-7. Epub 2022 Apr 12. Lucie Y Guo #  1   2 , Jing Bian #  3 , Alexander E Davis  4 , Pingting Liu  4 , Hannah R Kempton  3 , Xiaowei Zhang  3 , Augustine Chemparathy  3 , Baokun Gu  3 , Xueqiu Lin  3 , Draven A Rane  3 , Xiaoshu Xu  3 , Ryan M…

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Metagenomi Presents Findings on Novel Families of Ultra Small, CRISPR Enzymes at the Keystone Symposia Precision Genome Engineering Conference

EMERYVILLE, Calif., May 03, 2022–(BUSINESS WIRE)–Metagenomi, a genetic medicines company with a versatile portfolio of next-generation gene editing tools, presented findings on the identification of novel families of compact, programmable enzymes for genome editing at the Keystone Symposia Precision Genome Engineering and Emerging Cellular Therapies Conference, on April 30. The…

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Team develops method to increase gene editing efficiency while minimizing DNA deletion sizes

Targeting E. coli DNA pol I to DSBs increased the ratio of 1-bp deletions versus >1-bp deletions and TIS versus non-TIS. (A) Counteracting DNA resection (e.g. MRE11) by pol I fused to Cas9. The expected result is suppression of the MMEJ and SSA DNA repair pathways, which require DNA resection. (B)…

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Week ending April 23: longest patent process for Springfield inventors

The longest time between a patent being filed and granted in the week ending April 23 in Springfield was 1,670 days, according to the United States Patent and Trademark Office. The patent application was filed by Board of Trustees of Southern Illinois University for a tuning CRISPR/Cas9 activity with chemically…

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Enabling genome editing research | Scientist Live

As researchers using genome editing tools move from basic research to clinical settings, high-quality ancillary materials and documentation are critical. To help researchers meet stringent quality requirements, Thermo Fisher Scientific introduced the new GMP-manufactured Gibco CTS TrueCut Cas9 Protein. TrueCut Cas9 proteins are manufactured with United States Pharmacopeia standards in…

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Human stem cell modelling for heart disease of long QT syndrome

Abstract Sudden cardiac death (SCD) in otherwise healthy young people is a public health priority. Long QT syndrome (LQTS) is the commonest life-threatening cardiac arrhythmia contributing to SCD under 40 years old with an estimated prevalence of 1:2,000. Cardiac rhythm is driven by coordinated cardiac action potential, which is triggered…

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Unlocked enzyme structure shows how strigolactone hormone controls plant growth

Using a permanently unlocked form of the plant enzyme MAX3, UC Davis researchers are exploring the mechanism by which the plant hormone strigoractone regulates a massive network of genes controlling plant growth and architecture. Credit: Nitzan Shabek/UC Davis As sessile organisms, plants have to continually adapt their growth and architecture…

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Genome Editing Market Size Value US$ 16.98 billion by 2028; grow at a CAGR of 18.4%

According to our new research study on “Genome Editing Market Forecast to 2028 – COVID-19 Impact and Global Analysis – by Technology, Application, and End User,” the market is expected to grow from US$ 5.19 billion in 2021 to US$ 16.98 billion by 2028; it is estimated to grow at…

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CRISPR/Cas9 deletions induce adverse on-target genomic effects

Clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) have transformed genome engineering techniques. Numerous toolsets have been created to enable easy and efficient loss-of-function perturbations of functional genomic sites. Study: CRISPR/Cas9 deletions induce adverse on-target genomic effects leading to functional DNA in human cells. Image Credit: elenabsl/Shutterstock The CRISPR/Cas9 system’s elements…

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Genome Editing of Human Primary T Cells With Lipid Nanoparticles

By Reka Geczy, PhD, Aruna Balgi, Stella Park, Rita Zhao, Ethan Watt, Maggie Wong, Cooper Webb, Nikita Jain, PhD, Angela Zhang, PhD, Anitha Thomas, PhD, Samuel Clarke, PhD The expression of the chimeric antigen receptor (CAR) on T cells turns a patient’s cells into cell-based cancer therapies and has revolutionized…

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Research team follows nearly 8,000 paths towards better cell factories

Saccharomyces cerevisiae, SEM image. Credit: Mogana Das Murtey and Patchamuthu Ramasamy/CC BY-SA 3.0 Microorganisms that efficiently convert plant biomass into renewable biofuels and biochemicals play a major role in the sustainable society of the future. However, the efficiency of these microbial cell factories is inhibited by several compounds that are…

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Rat Model Market revenue worth USD 2.3 Billion by 2028,

Selbyville, Delaware, April 26, 2022 (GLOBE NEWSWIRE) — The rat model market value is projected to reach USD 2.3 billion by 2028, according to a new research report by Global Market Insights Inc. Growing consumption of personalized medicine and increasing number of clinical trials is expected to augment the market…

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CRISPR Technology Market Size, Share, Demand, Assessment & Opportunity Forecast 2021-2030

Global CRISPR Technology Market Size study, By Product and Service (Hand-Emplaced, Air-Delivered), By Applications (Seismic, Acoustic, Magnetic, Infrared), By End User (Security {Military, Public Security} Critical Infrastructure {Utilities, Industrial & Commercial Facilities}), and Regional Forecasts 2022-2028 Global CRISPR Technology Market is valued approximately USD 1097.66 million in 2021 and is anticipated…

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CRISPR Gene-Editing Market May See a Big Move by 2029

Global Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Gene-Editing Market Synopsis: The finest Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Gene-Editing Market research report contains the drivers and restraints for the market that are obtained with the help of SWOT analysis, and also shows all the recent developments, product launches,…

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Science Papers on Cancer Mutational Signatures, System to Deliver CRISRP-Cas9 Machinery Across Blood-Brain Barrier

By sequencing tumors from thousands of cancer patients and comparing them to similar datasets generated by other research groups, a team led by scientists from the University of Cambridge has identified new mutational signatures of cancer, while also uncovering details about organ-specific common and rare signatures. In the study, which…

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Global KRAS Inhibitors Market & Clinical Trials Forecast Report to 2028 – ResearchAndMarkets.com

DUBLIN–(BUSINESS WIRE)–The “Global KRAS Inhibitors Market & Clinical Trials Forecast 2028” report has been added to ResearchAndMarkets.com‘s offering. The advancement in clinical research has led to the identification of several oncogenes that are generally mutated in cancers. Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) is the most frequently mutated…

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03 Postdoctoral Fellowship at European Molecular Biology Laboratory (EMBL), Germany

European Molecular Biology Laboratory (EMBL), Germany invites online Application for various Postdoctoral Fellowship in their different Departments. We are providing a list of Postdoc Fellowship positions available at European Molecular Biology Laboratory (EMBL), Germany. Eligible candidate may Apply as soon as possible.   (01) Postdoctoral Fellowship Position Postdoc Fellowship Position…

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Oligonucleotide Pool Library Market 2021-2026 Detailed Analysis and Growth Strategies, Regional and Recent Scenario Analysis

The new study on the Global Oligonucleotide Pool Library Market 2020-2026 analyzes the crucial capabilities, key infrastructures, major organizations, evaluates measures to attain potential success in the Oligonucleotide Pool Library industry. Because of the in-depth overview of the world Oligonucleotide Pool Library market, the recent document allows investors, traders and…

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Researchers boost rice yield by overcoming tr

image: The mechanism of boosting rice yield by overcoming a trait trade-off beteen tiller number and panicle size view more  Credit: IGDB Crop breeding is largely constrained by tradeoffs among different agronomic traits. Since many of these tradeoffs are caused by gene pleiotropy, reducing gene pleiotropy may make it easier to…

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Identifying a new lipid metabolism gene

People with familial hypercholesterolemia, or FH, have very high levels of low-density lipoprotein cholesterol circulating in their blood due to aberrant LDL uptake by cells. With LDL levels elevated for prolonged periods, these patients are at increased risk for atherosclerotic cardiovascular disease. Transgelin is a protein that in humans is…

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Oligonucleotide Pool Library Market Report 2022 By Segmentations, Key Company Profiles, Types, Applications & Regional Forecasts to 2030

The Oligonucleotide Pool Library market report offers a deep dive assessment of the major trends influencing the expansion trajectory of Oligonucleotide Pool Library market. This research document is strategically curated with actionable insights on the pivotal aspects of the industry sphere to empower you with in depth market intelligence. It…

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Know Market Dynamics, Opportunities and Risks 2027

The Oligonucleotide Pool Library Market Size and Forecast to 2027, this report provides an analysis of the impact of the COVID19 epidemic on the key points influencing the growth of the market. In addition, SaaS Online Video Platforms market segments (by major players, types, applications, and major regions) outlook, company…

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CRISPRi for specific inhibition of miRNA clusters and miRNAs with high sequence homology

Bartel, D. P. MicroRNAs: Target recognition and regulatory functions. Cell 136, 215–233 (2009). CAS  Article  Google Scholar  Bassett, A. R. et al. Understanding functional miRNA–target interactions in vivo by site-specific genome engineering. Nat. Commun. 5, 4640 (2014). ADS  CAS  Article  Google Scholar  Moore, M. J. et al. miRNA–target chimeras reveal…

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Nature Papers on CRISPR-Cas9 Gene Editing, HSC Development

New details about the mechanism by which DNA is unwound so that its target sequence can be read during CRISPR-Cas9 gene editing are reported in this week’s Nature Structural & Molecular Biology. The study provides insights that may help in the development of improved genome editors. During CRISPR-Cas9 editing, Cas9…

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Scientists Are Using CRISPR Gene Editing to Try to Create Hypoallergenic Cats

There’s been a lot of buzz in the last few years about the perils of using CRISPR gene editing on human germline cells—the reproductive cells that determine the DNA of every other cell in the body. The idea of CRISPR babies (hypothetical as far as we know, except in the…

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sgRNA design tool for CRISPR/Cas9 experiments targeting transcription factor motifs

%PDF-1.7 % 1 0 obj >/Metadata 4 0 R/Pages 2 0 R/StructTreeRoot 3 0 R/Type/Catalog/ViewerPreferences 5 0 R>> endobj 4 0 obj >stream Microsoft® Word 2019 application/pdf Marta Kazimierska TransCRISPR – sgRNA design tool for CRISPR/Cas9 experiments targeting transcription factor motifs Microsoft® Word 2019 2022-03-31T12:15:15+02:00 2022-04-07T11:33:55-07:00 2022-04-07T11:33:55-07:00 uuid:D44C43A8-2A40-4F2D-8CFE-62419359C244 uuid:25f34f1a-1dd2-11b2-0a00-380000000000 endstream endobj…

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MAGeCK – BioGrids Consortium – Supported Software

AllHigh-Throughput SequencingGenomicsProteomicsVisualizationOther MAGeCK Description (Model-based Analysis of Genome-wide CRISPR-Cas9 Knockout) a computational tool to identify important genes from the recent genome-scale CRISPR-Cas9 knockout screens (or GeCKO) technology. Installation Use the following command to install this title with the CLI client: $ biogrids-cli install mageck Copy to clipboard Primary Citation* W….

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Researchers identify sustainable source of im

image: Monkey iPSCs with edited CCR5 locus growing in culture. view more  Credit: Image Credit: Saritha D’Souza, Wisconsin National Primate Research Center, USA Genetic engineering can make immune cells resistant to infection with human or monkey (simian) immunodeficiency virus (HIV and SIV, respectively). Recently, treatment with HIV-resistant immune cells has given…

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What is genetic engineering and how can it benefit healthcare?

Have you heard of purple tomatoes? Or golden rice? If you haven’t, you might be surprised to know that these have actually been created by scientists via genetic engineering. These are genetically modified foods, with purple tomatoes containing 10 times more antioxidants than a normal red tomato. On the other…

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Gann and Dharwadker are Recipients of Awards by the Society for In Vitro Biology

Photo Submitted (Right) Dominic Dharwadker, undergraduate student, Honors Biochemistry; (Left) Peter James Gann, doctoral student, Cell and Molecular Biology. A doctoral Fulbright scholar from the Cell and Molecular Biology (CEMB) Program and an undergraduate student in Honors Biochemistry from the Department of Chemistry and Biochemistry are winners…

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What are the PRLR-SLICK cattle?

Earlier this month, a type of short-haired cattle, called PRLR-SLICK, was added to a very short list of animals given a low-risk assessment by the FDA’s Center for Veterinary Medicine (CVM). What’s so special about these cattle is that they are the first intentional genomic alteration (IGA) in an animal…

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A transgene-free method for rapid and efficie

image: The reporter RNA enriched dual-sgRNA/CRISPR-Cas9 ribonucleoproteins (RE-DSRNP) method uses dual-sgRNA in combination with reporter RNA enriched RNPs (CRISPR-Cas9 ribonucleoproteins), which incorporates the precise targeting of dual-sgRNA systems with the increased editing rates but reduced off-target effects and cytotoxicity associated with reporter RNA-enriched RNPs, without the need for introducing transgenes, thus…

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AAV-delivered suppressor tRNA overcomes a nonsense mutation in mice

Wang, D., Tai, P. W. L. & Gao, G. Adeno-associated virus vector as a platform for gene therapy delivery. Nat. Rev. Drug Discov. 18, 358–378 (2019). CAS  PubMed  PubMed Central  Google Scholar  Li, C. & Samulski, R. J. Engineering adeno-associated virus vectors for gene therapy. Nat. Rev. Genet. 21, 255–272…

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Using CRISPR/Cas to develop biosafety materials

The diagnosis and treatment of viral infections, especially those that cause large-scale outbreaks, is urgently needed, as the lack of these measures allows epidemics to progress. A new Biosafety and Health study discusses the use of the highly efficient clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) technology to…

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BmPMFBP1 regulates the development of eupyrene sperm in the silkworm, Bombyx mori

This article was originally published here PLoS Genet. 2022 Mar 21;18(3):e1010131. doi: 10.1371/journal.pgen.1010131. Online ahead of print. ABSTRACT Sperm deliver the male complement of DNA to the ovum, and thus play a key role in sexual reproduction. Accordingly, spermatogenesis has outstanding significance in fields as disparate as infertility treatments and…

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Team develops new process for evaluating impacts of gene edits that alter rather than ‘knock out’ DNA code

CRISPR-associated protein Cas9 (white) from Staphylococcus aureus based on Protein Database ID 5AXW. Credit: Thomas Splettstoesser (Wikipedia, CC BY-SA 4.0) A new study from scientists at ChristianaCare’s Gene Editing Institute is advancing the safety and efficacy of using CRISPR gene editing in patient treatments by demonstrating how to identify and…

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A genome-scale screen for synthetic drivers of T cell proliferation

Abramson, J. S. et al. Transcend NHL 001: immunotherapy with the CD19-directed CAR T-cell product JCAR017 results in high complete response rates in relapsed or refractory B-cell non-Hodgkin lymphoma. Blood 128, 4192–4192 (2016). Google Scholar  Shifrut, E. et al. Genome-wide CRISPR screens in primary human T cells reveal key regulators…

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Sigma-Aldrich Files Opposition to CVC Substantive Preliminary Motion No. 1 to be Accorded Priority Benefit | McDonnell Boehnen Hulbert & Berghoff LLP

On February 18th, Sigma-Aldrich filed its Opposition to Junior Party’s (the University of California, Berkeley; the University of Vienna; and Emmanuelle Charpentier; collectively, “CVC”) Substantive Preliminary Motion No. 1 in Interference No. 106,132, asking the Patent Trial and Appeal Board for benefit of priority to U.S. Provisional Application No. 61/652,086,…

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Cutting Edge Agriculture Gene Editing with Cas-CLOVER

Blog Agriculture Biotechnology Though CRISPR-Cas enabled targeted genome engineering across a vast array of organisms, the system features major disadvantages. Frequent off-target mutagenesis, licensing restrictions and non-ideal economic license terms have inhibited commercial crop-science product upscaling, and, in many cases, entirely disqualified CRISPR’s use by many commercial crop developers. Seeking…

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Cho Cell Line Development & Engineering: Impact On Biopharmaceutical Production: Essay Example, 2571 words

1. Introduction The approval of Chinese hamster ovary (CHO) cell cultures for the manufacturing of protein therapeutic products modernized conventional medicine. CHO cells remained the workhorse for the production of monoclonal antibodies (mAbs) for the last at least 20 years for different reasons. First, CHO cells are easily adapting and…

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Establishment and Genetic Manipulation of Murine Hepatocyte Organoids

A long-term ex vitro 3D organoid culture system was established from mouse hepatocytes. These organoids can be passaged and genetically manipulated by lentivirus infection of shRNA/ectopic construction, siRNA transfection and CRISPR-Cas9 engineering. The liver is the biggest organ in mammals. It plays very important role in metabolism and detoxification. Although…

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Development of Cas12a-Based Cell-Free Small-Molecule Biosensors via Allosteric Regulation of CRISPR Array Expression

In nature, microbes have evolved different systems to sense external stimuli. Synthetic biology approaches (1) repurpose these systems as biosensors to specifically and sensitively detect various targets of interest. Although various highly sensitive and specific laboratory-based analytical methods (including high-performance liquid chromatography and mass spectrometry) can detect small-molecule targets, they…

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Intellia gets upgrade at Brookline amid ‘unchanged’ patent risk on CRISPR/Cas9 technology

Andy/iStock via Getty Images Intellia Therapeutics (NTLA -3.1%) was upgraded by Brookline Capital Management to Buy from Hold with a $91 price target. Brookline analyst Leah Cann said the patent risk is unchanged, but the valuation has become attractive. In February, the U.S. Patent and Trademark Office backed the Broad…

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EDIT Stock Escalated Afterhours Following Favorable Decision by USPTO

Editas Medicine, Inc. (EDIT) is a leading genome editing company engaged in translating the potential of the CRISPR/Cas12a and CRISPR/Cas9 genome editing platforms into a vigorous treatment pipeline for people with unmet medical needs. The price of EDIT stock during the regular trading on February 28, 2022, was $17.1 with…

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CVC maintains strong US patent portfolio covering CRISPR/Cas9 despite PTAB ruling, reports ERS Genomics

The PTAB decision makes a clear distinction between the CVC applications involved in the interference and CVC’s large portfolio of issued US patents that were not involved in this interference, stating that; “There is no dispute in this proceeding that the CVC inventors conceived of a generic sgRNA CRISPR-Cas9 system…

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Amide Internucleoside Linkages Are Well Tolerated in Protospacer Adjacent Motif-Distal Region of CRISPR RNAs

The development of CRISPR-Cas9 mediated gene editing technology is revolutionizing molecular biology, biotechnology, and medicine. However, as with other nucleic acid technologies, CRISPR would greatly benefit from chemical modifications that optimize delivery, activity, and specificity of gene editing. Amide modifications at certain positions of short interfering RNAs have been previously…

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A combinatorial CRISPR-Cas12a attack on HIV DNA: Molecular Therapy

CRISPR-Cas12a is an alternative class 2 gene-editing tool that may cause less off-target effects than the original Cas9 system. We have previously demonstrated that Cas12a attack with a single CRISPR RNA (crRNA) can neutralize all infectious HIV in an infected T cell line in cell culture. However, we demonstrated that…

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Chinese researchers develop fragrant sorghum with gene editing

A woman works in sorghum field in Yangling Village of Qicun Township in Zaozhuang City, east China’s Shandong Province, Sept. 7, 2021. (Photo by Sun Zhongzhe/Xinhua) Chinese researchers have successfully cultivated fragrant sorghum using CRISPR/Cas9 gene-editing technology. Aroma is an important quality in food. Jasmine rice, for instance, is popular…

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Drosophila carrying epilepsy-associated variants in the vitamin B6 metabolism gene PNPO display allele- and diet-dependent phenotypes

Pyridox(am)ine 5′-phosphate oxidase (PNPO; Enzyme Commission Number 1.4.3.5) is a rate-limiting enzyme in the synthesis of vitamin B6 (VB6) (1). Mutations in PNPO can cause neonatal epileptic encephalopathy, a devastating disease that usually leads to death if untreated (2). Recently, PNPO mutations have also been reported in patients with infantile…

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LAMP-CRISPR-Cas12a-lateral flow immunochromatographic strip | IDR

Introduction About 700,000 people die from “superbugs” globally every year. Antibiotic abuse is the culprit of extensive spread of superbugs. Currently, carbapenemase-producing organisms are among the most important pathogens of hospital infections. Especially the plasmid-mediated highly transmissible carbapenem-resistant Enterobacterales (CRE) has become an important public health issue of global concern….

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Validea Motley Fool Strategy Daily Upgrade Report – 2/26/2022

The following are today’s upgrades for Validea’s Small-Cap Growth Investor model based on the published strategy of Motley Fool. This strategy looks for small cap growth stocks with solid fundamentals and strong price performance. EXLSERVICE HOLDINGS, INC. (EXLS) is a mid-cap growth stock in the Business Services industry. The rating…

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(PDF) In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration | Shicheng Guo

(PDF) In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration | Shicheng Guo – Academia.edu Academia.edu no longer supports Internet Explorer. To browse Academia.edu and the wider internet faster and more securely, please take a few seconds to upgrade your browser. Academia.edu uses cookies to personalize content, tailor ads and…

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Molecular Biosensors Monitor Yeast Culture Intracellular Parameters

Much of the discussion around process analytics involves sensors: which ones are compatible, how they interface, and their in-, at-, and off-line capabilities. A recent paper from Chalmers University (Sweden) describes a platform for creating noninvasive biosensors from fluorophores engineered into yeast cells. The paper reports on how measuring several…

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Hot Plasmids – February 2022

Every few months we highlight a subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they’ll make it easier for you to find and use the plasmids you need. If you’d ever…

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The Clinical Importance of Immunogenetics

Immunogenetics describes the investigation of the genetics underpinning the immune system and its responses. Its overall goal is to identify the specific genes responsible for encoding immunological cells and molecules. DNA. Image Credit: vitstudio/Shutterstock.com Linking genetics with immune dysregulation Immunogenetics is highly pertinent to a plethora of…

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Butterfly eyespots evolved via cooption of an ancestral gene-regulatory network that also patterns antennae, legs, and wings

Although the hypothesis of gene-regulatory network (GRN) cooption is a plausible model to explain the origin of morphological novelties (1), there has been limited empirical evidence to show that this mechanism led to the origin of any novel trait. Several hypotheses have been proposed for the origin of butterfly eyespots,…

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Highly Efficient Genome Editing in Plant Protoplasts by Ribonucleoprotein Delivery of CRISPR-Cas12a Nucleases

Review doi: 10.3389/fgeed.2022.780238. eCollection 2022. Affiliations Expand Affiliations 1 Department of Plant Science and Landscape Architecture, University of Maryland, College Park, College Park, MD, United States. 2 College of Agriculture, Nanjing Agricultural University, Nanjing, China. 3 Integrated DNA Technologies, Coralville, IA, United States. 4 U.S. Horticultural Research Laboratory, USDA-Agricultural Research…

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The comparisonof the function of crRNA (cr-ribonucleic acid) and tracrRNA (trans-activating crRNA) in the CRISPR-Cas(clustered regularly interspaced short palindromic repeats-CRISPR associated protein 9)system. Introduction: The CRISPR-Cas system is a crucial defense system against foreign invaders, like bacteriophage, transposons, and plasmids in many prokaryotes. There are five genes present on it: tracr, Cas1, Crispr, Cas2, Cas9 on the CRISPR. The tracrRNA and the crRNA have a guiding function in the expression phase of the defense mechanism.

Chapter 17, Problem 11CONQ To review: The comparisonof the function of crRNA (cr-ribonucleic acid) and tracrRNA (trans-activating crRNA) in the CRISPR-Cas(clustered regularly interspaced short palindromic repeats-CRISPR associated protein 9)system. Introduction: The CRISPR-Cas system is a crucial defense system against foreign invaders, like bacteriophage, transposons, and plasmids in many prokaryotes….

Continue Reading The comparisonof the function of crRNA (cr-ribonucleic acid) and tracrRNA (trans-activating crRNA) in the CRISPR-Cas(clustered regularly interspaced short palindromic repeats-CRISPR associated protein 9)system. Introduction: The CRISPR-Cas system is a crucial defense system against foreign invaders, like bacteriophage, transposons, and plasmids in many prokaryotes. There are five genes present on it: tracr, Cas1, Crispr, Cas2, Cas9 on the CRISPR. The tracrRNA and the crRNA have a guiding function in the expression phase of the defense mechanism.

Sigma-Aldrich Files Substantive Preliminary Motion No. 1 to Deny Broad Priority Benefit to Its Earliest-filed Provisional Application | McDonnell Boehnen Hulbert & Berghoff LLP

On December 3rd, Senior Party Sigma-Aldrich filed its Substantive Preliminary Motion No. 1 in Interference No. 106,133 (which names the Broad Institute, Harvard University, and MIT (collectively, Broad) as Junior Party), asking the Patent Trial and Appeal Board to deny Broad benefit of its U.S. Provisional Application No. 61/736,527, filed…

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A Guide for Optimizing Arrayed CRISPR Screens

Loss-of-function (LOF) screens measure the phenotypic effects of gene disruptions, which helps researchers identify and validate gene targets for drug discovery. CRISPR-Cas9 technology has enabled great advances in LOF screening. While researchers typically use pooled CRISPR screens to assess fitness phenotypes, arrayed screens are amenable to a variety of functional…

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Decoding the dangers of ticks

It may now be possible to reduce tick-borne diseases, such as Lyme disease, thanks to two new gene-editing methods developed by researchers in the U.S. The new tools could be used to further understand tick biology, and potentially alter parts of the tick genome involved in harbouring and transmitting pathogens….

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CRISPR-Cas9 Gene Therapy for Duchenne Muscular Dystrophy

Ishino Y, Shinagawa H, Makino K, et al. Nucleotide sequence of the iap gene, responsible for alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product. J Bacteriol. 1987;169:5429–33. CAS  PubMed  PubMed Central  Google Scholar  Jansen R, van Embden JDA, Gaastra W, et al. Identification of genes…

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Broad Files Substantive Preliminary Motion No. 3 to Designate Claims as not Corresponding to Count in Interference No. 106,133 | McDonnell Boehnen Hulbert & Berghoff LLP

On December 3rd, Junior Party the Broad Institute, Harvard University, and MIT (collectively, Broad) filed its Contingent Preliminary Motion No. 3 in Interference No. 106,133 (which names Sigma-Aldrich as Senior Party), asking the Patent Trial and Appeal Board to designate certain claims deemed in the Declaration as corresponding to the…

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Sci-Hub | Cas9 versus Cas12a/Cpf1: Structure-function comparisons and implications for genome editing. Wiley Interdisciplinary Reviews: RNA, 9(5), e1481

Sci-Hub | Cas9 versus Cas12a/Cpf1: Structure-function comparisons and implications for genome editing. Wiley Interdisciplinary Reviews: RNA, 9(5), e1481 | 10.1002/wrna.1481 ◂ ↓ save Swarts, D. C., & Jinek, M. (2018). Cas9 versus Cas12a/Cpf1: Structure-function comparisons and implications for genome editing. Wiley Interdisciplinary Reviews: RNA, 9(5), e1481. doi:10.1002/wrna.1481  10.1002/wrna.1481 Read more…

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Requirement of Xk and Vps13a for the P2X7-mediated phospholipid scrambling and cell lysis in mouse T cells

Significance The extracellular concentration of adenosine triphosphate (ATP) reaches several hundred micromoles in the inflamed tissues or tumor environment. A high concentration of ATP activates P2X7, a purinergic receptor, and induces the formation of a nonselective cation channel, accompanied by reversible phosphatidylserine (PtdSer) exposure, leading to cell lysis. Here, we…

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Intellia (NTLA) falls 3.55% for February 11

Last Price $ Last Trade Change $ Change Percent % Open $ Prev Close $ High $ low $ 52 Week High $ 52 Week Low $ Market Cap PE Ratio Volume Exchange NTLA – Market Data & News Trade Intellia Therapeutics Inc (NASDAQ: NTLA), a Cambridge, Massachusetts, company,…

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Novel CRISPR platform to decode the immune system

Date: 11th February 2022 The immune system is a critical biological network of processes that protects an organism from disease, and depends on the ability to distinguish self from non-self, a role driven by antigens.  In humans, T cells respond to antigen stimulation together with the production of cytokines however,…

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Spatial components of molecular tissue biology

1. Okabe, Y. & Medzhitov, R. Tissue biology perspective on macrophages. Nat. Immunol. 17, 9–17 (2016). CAS PubMed  Google Scholar  2. Xia, C., Fan, J., Emanuel, G., Hao, J. & Zhuang, X. Spatial transcriptome profiling by MERFISH reveals subcellular RNA compartmentalization and cell cycle-dependent gene expression. Proc. Natl Acad. Sci. USA…

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Novel CRISPR Tool Activates Instead of Editing Human Immune Cell Genes

Scientists at Gladstone Institutes and UC San Francisco (UCSF) say they have co-opted the CRISPR-Cas9 system to forcibly activate genes—rather than edit them—in human immune cells. The method, known as CRISPRa, lets them discover genes that play a role in immune cell biology more thoroughly and rapidly than previously possible,…

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dCas9-VPR-mediated transcriptional activation of functionally equivalent genes for gene therapy

1. Dunbar, C. E. et al. Gene therapy comes of age. Science 359, eaan4672 (2018). PubMed Google Scholar  2. Wang, D., Tai, P. W. L. & Gao, G. Adeno-associated virus vector as a platform for gene therapy delivery. Nat. Rev. Drug Discov. 18, 358–378 (2019). CAS PubMed PubMed Central Google Scholar  3. Eid, A.,…

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Generation of NKX2.5 GFP Reporter Human iPSCs and Differentiation Into Functional Cardiac Fibroblasts

doi: 10.3389/fcell.2021.797927. eCollection 2021. Leyre López-Muneta  1 , Javier Linares  1 , Oscar Casis  2 , Laura Martínez-Ibáñez  3 , Arantxa González Miqueo  3   4 , Jaione Bezunartea  5 , Ana Maria Sanchez de la Nava  6   7 , Mónica Gallego  2 , María Eugenia Fernández-Santos  6   7 , Juan Roberto Rodriguez-Madoz  8 , Xabier L Aranguren …

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