Tag: cas9

Post-doctoral Fellow – Analyst Bioinformatics in Oklahoma City, OK for Oklahoma Medical Research Foundation

Details Posted: 20-Jan-22 Location: Oklahoma City, Oklahoma Type: Full Time Required Education: Doctorate Categories: Biomedical Engineering Years of Experience: Less than 2 Post-doctoral Fellow – Analyst Bioinformatics OverviewOMRF is an independent, not-for-profit biomedical research institute adjacent to the University of Oklahoma Health Sciences Center (OUHSC) campus in Oklahoma City. Oklahoma…

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A cell-based phenotypic library selection and screening approach for the de novo discovery of novel functional chimeric antigen receptors

Cell lines and culture Unless stated otherwise, all tumor target cell lines were sourced from the ATCC via LGC Standards. Human endogenous mesothelin (MSLN)-positive target cell lines H-226 (lung carcinoma, MSLN+, ATCC® CRL-5826™) and AsPC-1 (ATCC CRL-1682), and MSLN-negative Raji control cells (ATCC CCL-86) were cultured in RPMI-1640 Glutamax (Life Technologies,…

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Editas (EDIT) Focuses on Developing Gene-Editing Eye Drug

This story originally appeared on Zacks Editas Medicine, Inc. EDIT has made rapid progress in the development of its lead pipeline candidate, EDIT-101, which employs CRISPR gene editing, to treat Leber congenital amaurosis type 10 (LCA10) — a rare genetic illness that causes blindness. – Zacks The company is evaluating…

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Valine feeding reduces ammonia production through rearrangement of metabolic fluxes in central carbon metabolism of CHO cells

Aghamohseni H, Ohadi K, Spearman M, Krahn N, Moo-Young M, Scharer JM, Butler M, Budman HM (2014) Effects of nutrient levels and average culture pH on the glycosylation pattern of camelid-humanized monoclonal antibody. J Biotechnol 186:98–109. doi.org/10.1016/j.jbiotec.2014.05.024 CAS  Article  PubMed  Google Scholar  Altamirano C, Illanes A, Casablancas A, Gámez X,…

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Establishment of serum-free adapted Chinese hamster ovary cells with double knockout of GDP-mannose-4,6-dehydratase and GDP-fucose transporter

Aggarwal RS (2014) What’s fueling the biotech engine-2012 to 2013. Nat Biotechnol 32:32–39. doi.org/10.1038/nbt.2794 CAS  Article  PubMed  Google Scholar  Becker DJ, Lowe JB (2003) Fucose: biosynthesis and biological function in mammals. Glycobiology 13:41–53. doi.org/10.1093/glycob/cwg054 CAS  Article  Google Scholar  Chan KF, Shahreel W, Wan C et al (2016) Inactivation of GDP-fucose…

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Genetic strategies reverse pesticide resistance

Nature Communications i>In a new empirical study published in, a gene drive designed to bias heredity, called an allelic drive, was applied without pesticide treatment to create natural-level insects that were balanced with pesticide susceptibility. Population that has been shown to be recoverable. Credits: Bier Lab, University of California, San…

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CRISPR-Cas12a ribonucleoprotein-mediated gene editing in the plant pathogenic fungus Magnaporthe oryzae

. 2021 Dec 24;3(1):101072. doi: 10.1016/j.xpro.2021.101072. eCollection 2022 Mar 18. Affiliations Expand Affiliation 1 Department of Plant Pathology, Kansas State University, Manhattan, KS, USA. Free PMC article Item in Clipboard Jun Huang et al. STAR Protoc. 2021. Free PMC article Show details Display options Display options Format AbstractPubMedPMID . 2021 Dec…

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First time genome editing made possible on cells lining blood vessel walls

Credit: Pixabay/CC0 Public Domain The lab of Youyang Zhao, Ph.D., from Stanley Manne Children’s Research Institute at Ann & Robert H. Lurie Children’s Hospital of Chicago developed a unique nanoparticle to deliver genome editing technology, including CRISPR/Cas9, to endothelial cells, which are cells that line blood vessel walls. This is…

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Phospholipase A2 inhibitor and LY6/PLAUR domain-containing protein PINLYP regulates type I interferon innate immunity

Significance Interferon (IFN)-mediated antiviral responses serve as the first line of the host innate immune defense against viral infection. Here we identify a previously uncharacterized protein designated phospholipase A2 inhibitor and LY6/PLAUR domain-containing protein (PINLYP), which is essential for embryonic development and plays an important role in type I IFN…

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CRISPR AG (CRSP) falls 0.25% for December 28

Last Price $ Last Trade Change $ Change Percent % Open $ Prev Close $ High $ low $ 52 Week High $ 52 Week Low $ Market Cap PE Ratio Volume Exchange CRSP – Market Data & News Trade Today, CRISPR Therapeutics AG Inc’s (NASDAQ: CRSP) stock fell…

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PTAB Sets Motions and Times in Broad vs. Sigma Interference No. 106,133 | McDonnell Boehnen Hulbert & Berghoff LLP

Following a telephone conference held on August 16th (a transcript of which can be found here) between the Board and representatives of Junior Party the Broad Institute, Harvard University, and MIT (collectively, Broad) and Senior Party Sigma-Aldrich, the Board issued its Order on September 20th authorizing motions and setting times…

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Gene editing crucial for food security and environmental sustainability

World’s population is growing rapidly, and it is creating an array of challenges for food security. Fast-shrinking green cover to make ways for agriculture production has raised concerns in the wake of climate change showing its effects. There needs to be a viable and sustainable a mechanism that ensures adequate…

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Bioinformatics analysis of long non-coding RNA-associated competing endogenous RNA network in schizophrenia

1. Marder, S. R. & Cannon, T. D. Schizophrenia. N. Engl. J. Med. 381, 1753–1761. doi.org/10.1056/NEJMra1808803 (2019). Article  PubMed  CAS  Google Scholar  2. Keshavan, M. S. et al. Neuroimaging in Schizophrenia. Neuroimaging Clin. N. Am. 30, 73–83. doi.org/10.1016/j.nic.2019.09.007 (2020). Article  PubMed  Google Scholar  3. McCutcheon, R. A., Reis Marques, T….

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Translocations on chromosome 6 cause abnormalities during meiosis, resulting in fewer watermelon seeds

Credit: Unsplash / CC0 public domain Watermelon (Citrullus lanatus) is an economically important horticultural crop and one of the top five most widely consumed fresh fruits in the world. Compared to traditional seedless watermelons, seedless watermelons are preferred by consumers, show better fruit quality and bring greater economic benefits to…

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Editas Medicine Announces FDA Clearance of Investigational New Drug (IND) Application for EDIT-301 for the Treatment of Transfusion-Dependent Beta Thalassemia

EDIT-301 is in development as a transformative, one-time treatment for people living with transfusion-dependent beta thalassemia Editas Medicine will initiate a Phase 1/2 clinical trial in 2022 CAMBRIDGE, Mass., Dec. 20, 2021 (GLOBE NEWSWIRE) — Editas Medicine, Inc. (Nasdaq: EDIT), a leading genome editing company, today announced that the U.S….

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Regulating gene-editing technology

A chara, – As CEO of ERS Genomics, founded by Emmanuelle Charpentier, Nobel Prize winner for the discovery of Crispr/Cas9, I’d like to echo the sentiments of Dr Thomas McLoughlin (Letters, December 16th), who writes: “Crispr has the potential to revolutionise agriculture, environment and medicine for the benefit of people…

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sgRNA design for DLT gene editing using CRISPR-Cas9 and in-silico mutation prediction in Rice cv. Hawara Bunar

The DWARF AND LOW TILLERRING (DLT) gene is a transcription factor for a gene involved in Brassinosteroid (BR) biosynthesis. Manipulating BR biosynthesis will affect the height and tiller number of rice. CRISPR-Cas9 is an accurate tool to edit a gene sequence. The accuracy of site editing of the CRISPR-Cas9-mediated target…

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Science snapshots from Berkeley Lab

image: 3D image of melanin in a zebrafish sample captured by micro-computed tomography. view more  Credit: Spencer R. Katz and Daniel J. Vanselow, Penn State College of Medicine) Adapted from a UC Berkeley news release To date, CRISPR enzymes have been used to edit the genomes of one type of cell…

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Sigma-Aldrich and Broad Propose Preliminary Motions in Recent CRISPR Interference No. 106,133 | McDonnell Boehnen Hulbert & Berghoff LLP

The parties in Interference No. 106,133, namely Senior Party Sigma-Aldrich and Junior Party the Broad Institute, Harvard University, and MIT (collectively, “Broad”), filed their respective lists of proposed preliminary motions four days prior to their August 3rd teleconference with the Board to present their arguments for the Board to grant…

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Intellia (NTLA) gains 6.10% on Strong Volume December 15

Last Price $ Last Trade Change $ Change Percent % Open $ Prev Close $ High $ low $ 52 Week High $ 52 Week Low $ Market Cap PE Ratio Volume Exchange NTLA – Market Data & News Trade Intellia Therapeutics Inc (NASDAQ: NTLA) shares gained 6.10%, or…

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Rapid genome editing by CRISPR-Cas9-POLD3 fusion

Precision CRISPR gene editing relies on the cellular homology-directed DNA repair (HDR) to introduce custom DNA sequences to target sites. The HDR editing efficiency varies between cell types and genomic sites, and the sources of this variation are incompletely understood. Here, we have studied the effect of 450 DNA repair…

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Sigma-Aldrich Joins the CRISPR Interference Fray | McDonnell Boehnen Hulbert & Berghoff LLP

On June 21st,* the Patent Trial and Appeal Board declared two new interferences involving CRISPR technology.  The first, Interference No. 106,132, named Sigma-Aldrich as Senior Party and the University of California/Berkeley, the University of Vienna, and Emmanuelle Charpentier (collectively, “CVC”) as Junior Party, while the second, Interference No. 106,133 named…

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China credited with 4 of top 10 global engineering feats

A panoramic view of the Five-hundred-meter Aperture Spherical radio Telescope (FAST) in Pingtang, Guizhou Province. It is nicknamed Tianyan, or the Eye of Heaven, by amateur astronomers.(Photo: China Daily) China has four major engineering feats listed among this year’s Global Top Ten Engineering Achievements, according to the journal Engineering, one…

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Intellia Therapeutics Announces First Patient Dosed in Phase 1/2 Clinical Trial of NTLA-2002 for the Treatment of Hereditary Angioedema

NTLA-2002 is the first single-dose genome editing therapeutic candidate designed to prevent angioedema attacks in patients with hereditary angioedema (HAE) to enter clinical study CAMBRIDGE, Mass., Dec. 13, 2021 (GLOBE NEWSWIRE) — Intellia Therapeutics, Inc. (NASDAQ:NTLA), a leading clinical-stage genome editing company focused on developing curative therapeutics using CRISPR/Cas9 technology…

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DEFENCE THERAPEUTICS ACCUMTM BOOSTS BY 9-FOLD THE DELIVERY EFFECTIVENESS OF THE CRISPR/CAS9 PROTEIN TO TARGET CELLS

DGAP-News: Defence Therapeutics Inc. / Key word(s): Miscellaneous 13.12.2021 / 08:30 The issuer is solely responsible for the content of this announcement. CSE: DTC FSE: DTC USOTC: DTCFF PRESS RELEASE DEFENCE THERAPEUTICS ACCUMTM BOOSTS BY 9-FOLD THE DELIVERY EFFECTIVENESS OF THE CRISPR/CAS9 PROTEIN TO TARGET CELLS Vancouver, BC, Canada, December…

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Freedom to tinker: The UK is liberalising rules around gene-edited crops

Few consumed crops today are free of human meddling. Now, the UK is liberalising the rules around gene-edited crops In the wake of the UK’s departure from the EU, a change is coming. On 29 September, the Department for Environment, Food and Rural Affairs announced that by the end of…

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Large-scale genome-wide study reveals climate adaptive variability in a cosmopolitan pest

Genomic data The foundational resource for this study was a dataset of 40,107,925 nuclear SNPs sequenced from a worldwide sample of 532 DBM individuals collected in 114 different sites based on our previous project15. DNA was extracted from each of the 532 individuals using DNeasy Blood and Tissue Kit (Qiagen,…

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Science Papers Describe NanoProCas9 System, Inflammatory Protein Biomarkers, LncRNA Affects Muscle Tissue Formation in Aging

A conditionally activatable CRISPR-Cas9 prodrug system that can be leveraged for precision medicine is described in this week’s Science Advances. While CRISRP-Cas9 genome editing holds great therapeutic potential, the nonspecific distribution of Cas9 raises safety concerns relating to off-target activity at the tissue level. Aiming to overcome this limitation, Zhejiang…

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Watching CRISPR-Cas9 at Work in Real-Time

Detecting the activity of CRISPR gene editing tools in organisms with the naked eye and an ultraviolet flashlight is now possible using technology developed at the Department of Energy’s Oak Ridge National Laboratory. Scientists demonstrated these real-time detection tools in plants and anticipate their use in animals, bacteria and fungi…

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PJSC Sberbank : Sber holds a session on the future of basic and applied sciences on Congress of Young Scientists

At the Congress of Young Scientists, Sber has held a session called “The Scientific Breakthroughs We are About to See in the Next Decade: Life Science, Physical World, a Digital Universe” with a round table that addressed the prospects for the key fields of basic and applied sciences. The discussion…

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New biosensors shine a light on CRISPR gene editing — ScienceDaily

Detecting the activity of CRISPR gene editing tools in organisms with the naked eye and an ultraviolet flashlight is now possible using technology developed at the Department of Energy’s Oak Ridge National Laboratory. Scientists demonstrated these real-time detection tools in plants and anticipate their use in animals, bacteria and fungi…

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China science, technology news summary — Dec. 9

BEIJING, Dec. 9 (Xinhua) — The following is a summary of published science and technology news of China. LIVE CLASS FROM SPACE A special lecture kicked off Thursday afternoon at China’s space station, given by the Shenzhou-13 crew members Zhai Zhigang, Wang Yaping and Ye Guangfu to students on Earth….

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New biosensor sheds light on CRISPR gene editing

ORNL’s biosensor system reveals the CRISPR activity of poplar plants that glow bright green under UV light compared to normal plants that appear red.Credits: Guoliang Yuan / ORNL, US Department of Energy Using technology developed at the Oak Ridge National Laboratory of the Department of Energy, it is now possible…

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New biosensors shine a light on CRISPR gene editing

ORNL’s biosensor system reveals CRISPR activity in poplar plants, which glow bright green under ultraviolet light, compared to normal plants, which appear red. Credit: Guoliang Yuan/ORNL, U.S. Dept. of Energy Detecting the activity of CRISPR gene editing tools in organisms with the naked eye and an ultraviolet flashlight is now…

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A Mutation-Agnostic Hematopoietic Stem Cell Gene Therapy for Metachromatic Leukodystrophy

This article was originally published here CRISPR J. 2021 Dec 8. doi: 10.1089/crispr.2021.0075. Online ahead of print. ABSTRACT Metachromatic leukodystrophy (MLD) is a rare genetic disorder caused by mutations in the Arylsulfatase-A (ARSA) gene. The enzyme plays a key role in sulfatide metabolism in brain cells, and its deficiency leads…

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Nanotechnology for genome editing in multiple muscles simultaneously

Fig. 1: LNP-mediated Luc-mRNA or CRISPR-Cas9 mRNA/sgRNA delivery into muscle tissue. a Schematic illustration of LNP-CRISPR. Either Luc mRNA or Cas9 mRNA/sgRNA is encapsulated into LNP that consists of TCL053, DPPC (Dipalmitoylphosphatidylcholine), PEG-DMG (Polyethylene glycol-dimyristoyl glycerol), and cholesterol. b Chemical structure of the newly synthesized ionizable lipid, TCL053. c Representative…

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Stem cell study paves way for gene editing to produce cultured meat

Scientists have obtained stem cells from pigs, sheep, and cattle without the need for serum, feeder cells, or antibiotics to pave the way for “manufacturing cell-cultured meat and breeding enhanced livestock”. The researchers for the study were mainly from the University of Nottingham’s School of Biosciences in the UK, in…

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Scientists Genetically Edit Mice To Have Female Only Litters With 100 Percent Efficiency

Scientists have genetically edited mice so they only give birth to litters of all male or female offspring. Tests showed the technology was 100 percent effective, and researchers believe it could be used to vastly improve animal welfare in laboratory tests and agriculture. Often in farming or laboratory studies on…

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Chinese scientists develop precise gene-editing delivery tool

Chinese scientists have developed a targeted delivery system that can bring gene-editing tools to colon cells, offering a precise cure for inflammatory bowel disease. The study, published on Thursday in the journal Science Advances, reported a CRISPR-Cas9 prodrug nanosystem that can transport a gene-editing protein exclusively to inflammatory lesions in…

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CRISPR Pioneers Doudna and Banfield Introduce Microbiome Community Editing

CRISPR systems have been developed, to date, to edit the genes of one type of cell at a time. Now, a new technique has been characterized that can add or modify genes within a community of many different species simultaneously, opening the door to the idea of “community editing.” The…

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FuturaGene Secures License to CRISPR-Cas9 Technology to Develop Sustainable Varieties of Eucalyptus with Improved Productivity, Stress Resistance and Fiber Quality

Agreement with Corteva Agriscience and Broad Institute of MIT and Harvard grants FuturaGene access to gene editing technology to research and develop varieties of eucalyptus that are adapted to climate change SAO PAULO, Brazil, December 08, 2021–(BUSINESS WIRE)–FuturaGene, a wholly owned subsidiary of world-leading eucalyptus pulp producer, Suzano, will use…

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Boundary-pushing research projects boosted by President’s Excellence Fund | Imperial News

Projects making nanomagnetic computers, sustainable packaging, improved vaccine carriers, and cut-and-paste chemistry have won up to £250k each. Four teams have received funds from the Excellence Fund for Frontier Research (EFFR) this year which are awarded as part of the College’s strategic aim of acting courageously. The awards are designed to…

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CRISPR/Cas9 gene editing boosts effectiveness

image: Ultrasound irradiation of a tumor causes a lipid nanoparticle (drawing shown above) to release harmful ROS and a CRISPR/Cas9 gene-editing system. view more  Credit: Credit: Adapted from ACS Central Science 2021, DOI: 10.1021/acscentsci.1c01143 Sonodynamic therapy uses ultrasound in combination with drugs to release harmful reactive oxygen species (ROS) at the…

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Hyperexcitability and Pharmacological Responsiveness of Cortical Neurons Derived from Human iPSCs Carrying Epilepsy-Associated Sodium Channel Nav1.2-L1342P Genetic Variant

Abstract With the wide adoption of genomic sequencing in children having seizures, an increasing number of SCN2A genetic variants have been revealed as genetic causes of epilepsy. Voltage-gated sodium channel Nav1.2, encoded by gene SCN2A, is predominantly expressed in the pyramidal excitatory neurons and supports action potential (AP) firing. One…

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MicroRNA-181a Deficiency Results in Transcriptome-Wide Cell-Specific Changes in The Kidney and Increases Blood Pressure

The micro ribonucleic acid (microRNA) [MiR-181a] is downregulated in the kidneys of rodents and patients suffering from high blood pressure (BP). The miR-181a is a posttranslational inhibitor of renin articulation in vitro, yet pleiotropic systems by which miR-181a may impact BP are unclear. Whether cancellation of miR-181a/b-1 in vivo changes…

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Is Genome Editing the Microbiome Just Around the Corner?

To date, CRISPR enzymes have been used to edit the genomes of one type of cell at a time: They cut, delete or add genes to a specific kind of cell within a tissue or organ, for example, or to one kind of microbe growing in a test tube. Now,…

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the spacer of sgrna will hybridize with this sequence of dna from the fragment shown below?

James Guys, does anyone know the answer? get the spacer of sgrna will hybridize with this sequence of dna from the fragment shown below? from EN Bilgi. Solved The spacer of sgRNA will hybridize with this sequence Answer to Solved The spacer of sgRNA will hybridize with this sequence Do…

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Optimizing sgRNA to Improve CRISPR/Cas9 Knockout Efficiency: Special Focus on Human and Animal Cell

Review doi: 10.3389/fbioe.2021.775309. eCollection 2021. Affiliations Expand Affiliations 1 Plant Biotechnology Department, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran. 2 Department of Psychotherapy, Pirogov Russian National Research Medical University, Moscow, Russia. 3 Medical Faculty, Russian State Social University, Moscow, Russia. 4 Department of Prosthetic Dentistry, I. M….

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Inequitable Conduct by Senior Party Broad Alleged in Interference No. 106,115 (and PTAB May Finally Hear Evidence About It) | McDonnell Boehnen Hulbert & Berghoff LLP

An enduring and persistent (albeit until now unresolved) issue in the patent interferences involving the Broad Institute, Harvard University, and MIT (collectively, “Broad”) as Senior Party and the University of California/Berkeley, the University of Vienna, and Emmanuelle Charpentier (collectively, “CVC”) as Junior Party has been the question of whether Broad…

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Using CRISPR/Cas9 to Epigenetically Activate or Deactivate Genes

Since the discovery of the CRISPR-Cas9 system, targeting DNA more precisely for genetic editing has become a lot easier. However, removing part of the genetic code may not be necessary in all cases, especially if there were a way to switch off a gene of interest instead. Thankfully, scientists have…

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Stem cell study paves way for manufacturing cultured meat — ScienceDaily

Scientists have for the first time obtained stem cells from livestock that grow under chemically defined conditions, paving the way for manufacturing cell cultured meat and breeding enhanced livestock. Researchers from the University of Nottingham’s School of Biosciences, together with colleagues at the Universities of Cambridge, Exeter Tokyo and Meiji…

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New high fidelity CRISPR Cas9 protein

For research applications requiring highly precise genome editing, including engineering CAR T cells and creating cellular models for disease discovery, the Invitrogen TrueCut HiFi Cas9 protein significantly minimises off-target events while retaining maximum on-target editing efficiency. Thermo Fisher Scientific introduced the new protein to complement its growing portfolio of CRISPR genome…

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CRISPR-Cas9, the unwitting revolutionary – The McGill Tribune

Bacteria get a bad rap, and often deservedly so: Different strains cause a range of infections and diseases, including pneumonia, strep throat, and tuberculosis. However, any well-researched health food advocate can list the many benefits of the bacteria present in yogurt, and your local pub would be doomed without the…

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SOLVED:describe how the sgRNA is used to specify a target site for Cas9 along a chromosome?

The Elements of Life In biology, the elements of life are the essential building blocks that make up living things. They are carbon, hydrogen, nitrogen, oxygen, phosphorus, and sulfur. The first four of these are the most important, as they are used to construct the molecules that are necessary to…

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Semipublic Intellectual Sessions: What Comes After CRISPR?

On November 5, 2021, the Los Angeles Review of Books (LARB) hosted five scholars to discuss genome editing technologies, the systems within which they emerge and are deployed, and what they teach us about the values of our society. This panel was organized by Julien Crockett and Michele Pridmore-Brown as…

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Genetic variant linked to Amish community could pave way for new heart disease therapies

Scientists have known about rare and common genetic variants that influence cardiovascular disease, but few gene mutations have been found to impact more than one risk for coronary artery disease. That gap in knowledge has limited the development of new treatments targeted to individual risk factors. Now, scientists at the…

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Worldwide Rat and Mouse Model Industry to 2028

Dublin, Dec. 06, 2021 (GLOBE NEWSWIRE) — The “Rat And Mouse Model Market Size, Share & Trends Analysis Report By Type (Knockout, Knock-in), By Technology, By Service (Breeding, Rederivation), By Application (COVID-19, CVS), By End-use, And Segment Forecasts, 2021 – 2028” report has been added to ResearchAndMarkets.com‘s offering. The global…

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CRISPRing the microbiome is just around the corner

To successfully edit genes within multiple members of a microbial community, UC Berkeley scientists had to develop two new methods: Environmental Transformation Sequencing (ET-Seq), top, which allowed them to assess the editability of specific microbes; and DNA-editing all-in-one RNA-guided CRISPR-Cas transposase (DART), which allowed highly specific targeted DNA insertion into…

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Gene editing used to create all-male or all-female mice litters

Researchers from the Francis Crick Institute and the University of Kent used gene-editing technologies to create male-only and female-only mice litters, according to a study published in Nature Communications (1). The authors also suggested ways in which this method could be used to improve animal welfare in scientific research and…

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Therapeutic Potential of Exosomal circRNAin RA

Introduction Rheumatoid arthritis (RA) is a chronic inflammatory disease characterized by synovial pannus formation resulting from angiogenesis accompanied by inflammation, which leads to cartilage and bone destruction.1 The inflammatory state in RA is maintained by the transport of inflammatory cells and oxygen to the synovium through enhanced angiogenesis. Therefore, angiogenesis…

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advantages and disadvantages of CRISPR-Cas-based vs. patient-derived iPSC models

Introduction Generating cellular and mouse models for neurological diseases enables us to uncover disease mechanisms as well as develop therapeutic approaches also for neurogenetic diseases that are difficult to treat. Yet, neurological diseases are hard to model due to highly complex disease mechanisms and often age-dependent disease onset. Current animal…

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Gene-editing used to create single sex mice l

image: The black coat of the mouse refers to the genetically modified cells, the white is the non-modified cells. The researchers breed these animals to produce offspring which are 100% genetically modified, containing one half of CRISPR-Cas9. view more  Credit: The Francis Crick Institute Francis Crick Institute press release Under strict…

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Gene Editing Can Create Single-Sex Mice Litters With 100 Percent Efficiency

Researchers at the Francis Crick Institute and the University of Kent have employed gene-editing technology to create single-sex litters of mice with 100 percent efficiency, a breakthrough that could lead to the dramatic reduction of culling in both medical and agricultural settings. The work, published in Nature Communications, approaches the…

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CRISPR Cas-9 Used To Create Single Sex Mice Litters

Scientists at the Francis Crick Institute, in collaboration with University of Kent, have used gene editing technology to create female-only and male-only mice litters with 100% efficiency. This proof of principle study, published in Nature Communications today (Friday 3rd December), demonstrates how the technology could be used to improve animal welfare in scientific…

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Lab animals: Gene-editing technology is used to create female-only and male-only mice litters

Single-sex litters of mice comprising only either female or male pups have been produced by means of so-called CRISPR-Cas9 gene editing technology. The technique, developed by experts at the Francis Crick Institute and the University of Kent, works by inactivating embryos of one sex shortly after fertilisation.  It could be used to…

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Gene-editing used to create single sex mice litters, 100% efficiency

Scientists at the Francis Crick Institute, in collaboration with University of Kent, have used gene editing technology to create female-only and male-only mice litters with 100% efficiency. This proof of principle study, published in Nature Communications today (Friday 3rd December), demonstrates how the technology could be used to improve animal…

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Gene-editing used to create single sex mice litters, 100% efficiency — ScienceDaily

Scientists at the Francis Crick Institute, in collaboration with University of Kent, have used gene editing technology to create female-only and male-only mice litters with 100% efficiency. This proof of principle study, published in Nature Communications today (Friday 3rd December), demonstrates how the technology could be used to improve animal…

Continue Reading Gene-editing used to create single sex mice litters, 100% efficiency — ScienceDaily

Gene-Editing Used To Create Single Sex Mice Litters With 100% Efficiency

The black coat of the mouse refers to the genetically modified cells, the white is the non-modified cells. The researchers breed these animals to produce offspring which are 100% genetically modified, containing one half of CRISPR-Cas9. Credit: The Francis Crick Institute Scientists at the Francis Crick Institute, in collaboration with…

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Gene editing used to create littermates in male and female mice

The black coat of mice refers to transgenic cells and the white coat refers to unmodified cells. Researchers breed these animals to produce 100% genetically modified offspring that contain half of CRISPR-Cas9. Credit: Francis Crick Institute Scientists at the Francis Crick Institute, in collaboration with the University of Kent, used…

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The future of CRISPR is now

It has been more than a year since Markus Mapara, MD, a professor of medicine and director of blood and marrow transplantation at Columbia University Irving Medical Center in New York, first used an experimental CRISPR gene-editing treatment in a patient with sickle cell disease, an inherited blood disorder that…

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Genetically Steady and Cost-effective Gene Knockout Stable

Creative Biogene creatively develops a series of genetically steady and cost-effective gene knockout stable cell lines products for domestic and overseas clients. New York, United States – December 02, 2021—With years of experience in the gene-engineering field, Creative Biogene has built a team of experts to perform genome editing with…

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Investigators use gene therapy treatment of Fuchs in animal models

Fuchs endothelial corneal dystrophy is a progressive inherited condition that ultimately results in corneal edema and loss of vision. Up to now, treatment of patients with advanced disease involves corneal transplantation surgery. However, gene therapy may be a future option for these patients. A team of investigators led by Hironori…

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$17 million will launch trial of CRISPR cure for sickle cell disease

Dr. Mark Walters of UCSF Benioff Children’s Hospital Oakland explains how a CRISPR cure for sickle cell disease would benefit patients. (Video of UCSF) A small clinical trial of a CRISPR cure for sickle cell disease, approved earlier this year by the U.S. Food and Drug Administration, has received $17…

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Filipina scientist bags silver for research on malaria in competition in Sweden

Filipina scientist Sophia Raine Hernandez won second place at the Forskar Grand Prix in Sweden where she presented in exactly four minutes a way to defeat the parasite that causes malaria, a disease that kills hundreds of thousands of people every year. Using the CRISPR-Cas9, a tool that allows scientists…

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Mg2+-dependent conformational rearrangements of CRISPR-Cas12a R-loop complex are mandatory for complete double-stranded DNA cleavage

Significance CRISPR-Cas12a has emerged as attractive molecular scissors alternative to Cas9 owing to its unique features including fewer off-target effects, an alternative protospacer-adjacent motif sequence, pre-CRISPR RNA processing activity, and indiscriminate single-stranded DNase activity. However, despite these advantages, Cas12a has not been well utilized as recently reported base and prime…

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Sickle Cell Clinical Trial Aims to Cure Disease by Correcting Patient’s Mutated Gene

UCSF Benioff Children’s Hospital Oakland has received the largest research trial grant in its history to launch an innovative clinical trial that aims to cure sickle cell disease. The study, which plans to enroll its first patient later this year, will use CRISPR-Cas9 gene-editing technology on patients’ own blood stem…

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New method can precisely, rapidly correct genetic alterations in patient cells

Researchers in the University of Helsinki and University Hospital Helsinki have developed a method to precisely and rapidly correct genetic alterations in the cultured patient cells. The method produces genetically corrected autologous pluripotent stem cells from a 2-3 mm skin biopsy from patients with different genetic diseases. The corrected stem…

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Cellosaurus cell line HEK293T-CAF40-null (CVCL_A5EE)

Cell line name HEK293T-CAF40-null Synonyms CAF40-null HEK293T Accession CVCL_A5EE Resource Identification Initiative To cite this cell line use: HEK293T-CAF40-null (RRID:CVCL_A5EE) Comments Doubling time: ~30-40 hours (DSMZ).Knockout cell: Method=CRISPR/Cas9; HGNC; 10445; CNOT9.Transfected with: UniProtKB; P00552; Transposon Tn5 neo.Transformant: NCBI_TaxID; 28285; Adenovirus 5.Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) [tsA].Derived from sampling…

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A new technique can swiftly fix genetic changes in cells of patients

Researchers at the University of Helsinki, University Hospital Helsinki have devised an approach to rapidly correct genetic defects in cultured cells of patients. The procedure produces genetically corrected autologous stem cells from a 3 mm skin biopsy from patients with different genetic diseases. The stem cells that have been corrected…

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New method helps correct genetic alterations in the cultured patient cells rapidly

Researchers in the University of Helsinki and University Hospital Helsinki have developed a method to precisely and rapidly correct genetic alterations in the cultured patient cells. The method produces genetically corrected autologous pluripotent stem cells from a 2-3 mm skin biopsy from patients with different genetic diseases. The corrected stem…

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New High Fidelity CRISPR Cas9 Protein Reduces Off-target Effects for More Precise Genome Editing

CRISPR genome editing is inherently messy, often resulting in edits in unintended locations. Limiting off-target effects is critical for applications such as translational research, drug screening and drug modeling as researchers look down the road to future therapeutic development. The Invitrogen TrueCut HiFi Cas9 Protein demonstrates near complete elimination of…

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Nobel Laureate Dr. Jennifer Doudna and Groundbreaking Applications of CRISPR

In 2011, Dr. Jennifer Doudna began studying an enzyme called Cas9. Little did she know, in 2020 she would go on to win the Nobel Prize in Chemistry along with Emmanuelle Charpentier for discovering the powerful gene-editing tool, CRISPR-Cas9. Today, Doudna is a decorated researcher, the Li Ka Shing Chancellors…

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Gene Knock-in by CRISPR/Cas9 and Cell Sorting in Macrophage and T Cell Lines

This article was originally published here J Vis Exp. 2021 Nov 13;(177). doi: 10.3791/62328. ABSTRACT Functional genomics studies of the immune system require genetic manipulations that involve both deletion of target genes and addition of elements to proteins of interest. Identification of gene functions in cell line models is important…

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A CRISPR/Cas12a-based DNAzyme visualization system for rapid, non-electrically dependent detection of Bacillus anthracis

Introduction Anthrax, a serious zoonotic disease, is caused by Bacillus anthracis. Because B. anthracis spores can be used as biological warfare and terrorism agents, the development of sensitive, efficient detection methods for this pathogen is important and urgently needed. Currently, B. anthracis is detected by identifying its vegetative cells and…

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New route to producing antibiotics

Scientists have discovered a new route to produce complex antibiotics exploiting gene editing to re-programme pathways to future medicines urgently required to combat antimicrobial resistance, treat neglected diseases and tackle future pandemics. Researchers from The University of Manchester have discovered a new way of manipulating key assembly line enzymes in…

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Investing in Genomics

The world’s rarest and most debilitating diseases can potentially be cured thanks to genomics and gene editing breakthroughs. And this next-generation wave of technologies and medicines presents a hugely exciting opportunity for investors. Innovation is hot right now, and the cost of sequencing a human genome has fallen drastically, making…

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Gut microbiome genetically edited in live mice for the first time

Scientists at the University of California San Francisco (UCSF) have successfully edited the genome of bacteria in the gut microbiome of living mice, for the first time. Bacteria-hunting viruses are loaded with the CRISPR gene-editing system, in a breakthrough that could help manipulate the ratio between different bacterial species to…

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Batubara

Induced pluripotent stem cells and genome editing technology as therapeutic strategies for Duchenne muscular dystrophy Irwan Saputra Batubara Abstract Duchenne muscular dystrophy (DMD) is a rare genetic, progressiveand devastating skeletal and cardiac muscle disorder due to mutationof the dystrophin gene that affects 1 in 3500 young males. Currently,there is no…

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Molecular Mechanisms of Cereblon-Interacting Small Molecules in Multiple Myeloma Therapy

This article was originally published here J Pers Med. 2021 Nov 11;11(11):1185. doi: 10.3390/jpm11111185. ABSTRACT Thalidomide analogues (or immunomodulatory imide drugs, IMiDs) are cornerstones in the treatment of multiple myeloma (MM). These drugs bind Cereblon (CRBN), a receptor for the Cullin-ring 4 ubiquitin-ligase (CRL4) complex, to modify its substrate specificity….

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CRISPR-Created Model of Fabry Disease Sparks New Insights

Kidney organoids designed with the same mutation as that present in Fabry disease can help investigators better understand how the disease functions, authors suggested. A new report makes the case that stem-cell-derived kidney organoids can provide a meaningful model for understanding how Fabry disease affects the body and for developing…

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A human fetal liver-derived infant MLL-AF4 acute lymphoblastic leukemia model reveals a distinct fetal gene expression program

This article was originally published here Nat Commun. 2021 Nov 25;12(1):6905. doi: 10.1038/s41467-021-27270-z. ABSTRACT Although 90% of children with acute lymphoblastic leukemia (ALL) are now cured, the prognosis for infant-ALL remains dismal. Infant-ALL is usually caused by a single genetic hit that arises in utero: an MLL/KMT2A gene rearrangement (MLL-r)….

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BHLH11: Active Repressor for Iron Toxicity in Arabidopsis

Iron (Fe) is an indispensable microelement for plant growth and development, but Fe excess can be toxic to plants. To maintain Fe homeostasis, plants must sense the environmental Fe concentration and fine-tune the expression of Fe uptake-associated genes accordingly. Previous studies have shown that bHLH11 is a negative transcription factor…

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New research details using gene editing to make stronger antibiotics

New research has been published in Nature Communications, describing how a team of scientists from the University of Manchester used gene editing to manufacture new antibiotics. The team used CRISPR-Cas9 gene editing to create new non-ribosomal peptide synthetase (NRPS) enzymes, critical in the natural production of antibiotics like penicillin. Previously,…

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An electrochemical protocol for CRISPR-mediated gene-editing of sheep embryonic fibroblast cells

Genetic engineering of farm animals is commonly carried out via cell-mediated transfection followed by somatic cell nuclear transfer. However, efficient transfer of exogenous DNA into ovine embryonic fibroblast (EF) cells without compromising cell viability have remained a challenging issue. Here, we aimed to develop a protocol for electrotransfection of sheep…

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Scientists produce new antibiotics by gene editing

Scientists have discovered a new route to produce complex antibiotics exploiting gene editing to re-programme pathways to future medicines urgently required to combat antimicrobial resistance, treat neglected diseases and tackle future pandemics. Researchers from The University of Manchester have discovered a new way of manipulating key assembly line enzymes in…

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Breeder Q&A: All about basil

While basil is popular with consumers and economically important for the industry, the culinary herb has yet to benefit from the intensive research treatment enjoyed by crops such as corn, wheat, and soy. It is also no secret that the pipeline of new degreed or experienced talent simply is not…

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ecDNA hubs drive cooperative intermolecular oncogene expression

1. Wu, S. et al. Circular ecDNA promotes accessible chromatin and high oncogene expression. Nature 575, 699–703 (2019). 2. Gorkin, D. U., Leung, D. & Ren, B. The 3D genome in transcriptional regulation and pluripotency. Cell Stem Cell 14, 762–775 (2014). CAS  PubMed  PubMed Central  Google Scholar  3. Zheng, H….

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Infecting Gut Microbes with CRISPR-loaded Virus Demonstrates Potential for Microbiome Gene Editing

Escherichia coli (E. Coli) bacteria. In a first, researchers at the UC San Francisco have successfully used the DNA-editing system, CRISPR to alter the genomes of bacteria living in the guts of mammals, a development which stands to advance our understanding of the microbiome and could eventually pave the way…

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AstraZeneca opens new advanced therapeutics R&D facility in Cambridge

The pharma giant said the new £1bn (US$1.3bn) research and development center will include advanced robotics, high-throughput screening and AI-driven technology. A spokesperson for AstraZeneca told BioPharma-Reporter that the facility aims to pioneer a faster and more agile approach to developing medicines, in order to fully deliver patient-centered precision medicine…

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CRISPR Reveals how Hox Genes Controls Appearance

New findings utilizing CRISPR-Cas9 gene editing in drosophila demonstrate the ‘scaffold’ role of Hox genes in the development of anatomical appearance.  Following on from the ‘McGinnis experiment’ 30-years ago, Ankush Auradkar, William McGinnis’ mentee, has led a study on Hox genes, alongside senior author Ethan Bier (both University of California San Diego; UCSD, CA, USA). The researchers used CRISPR-Cas9 gene-editing technology to investigate the role…

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