Tag: CHO

doi.org/10.1016/0360-3016(89)90304-0Get rights and content Abstract A cell line, CHO-Chlr, which has elevated levels of glutathione-S-transferase (GST), is resistant to damage caused by bi-functional nitrogen mustards. This cell line has a similar radiation sensitivity to and value of oxygen enhancement ratio as the wild type cells, CHO-KI, from which the mutant…

doi.org/10.1016/0014-4827(83)90345-2Get rights and content Abstract The ultrastructural localization of small nuclear RNA (snRNA) was studied by EM autoradiography in Chinese Hamster Ovary (CHO) cells. Conditions were set where most (>85 %) of the nuclear [3H]uridine label consisted of snRNA, the most abundant species being U1, U2 and the nucleolar species…

Aghamohseni H, Ohadi K, Spearman M, Krahn N, Moo-Young M, Scharer JM, Butler M, Budman HM (2014) Effects of nutrient levels and average culture pH on the glycosylation pattern of camelid-humanized monoclonal antibody. J Biotechnol 186:98–109. doi.org/10.1016/j.jbiotec.2014.05.024 CAS  Article  PubMed  Google Scholar  Altamirano C, Illanes A, Casablancas A, Gámez X,…

Welcome from WaiMasterClass 2022 by Women in AI Myanmar! Women in AI Myanmar is hosting WaiMasterClass DataScience Series in 2022. There will be a total of 4 classes for this WaiMasterClass DataScience Series. This is our second event in Datascience series. In event 2, Aye Hninn Khine and Cho Zin…

Aggarwal RS (2014) What’s fueling the biotech engine-2012 to 2013. Nat Biotechnol 32:32–39. doi.org/10.1038/nbt.2794 CAS  Article  PubMed  Google Scholar  Becker DJ, Lowe JB (2003) Fucose: biosynthesis and biological function in mammals. Glycobiology 13:41–53. doi.org/10.1093/glycob/cwg054 CAS  Article  Google Scholar  Chan KF, Shahreel W, Wan C et al (2016) Inactivation of GDP-fucose…

Engineering of lipid-metabolism in CHO cells by over-expression of SCD1AbstractIntroduction–Overall aim is to investigate how SCD1 over-expression influences target recombinant protein expression, as well as how temperature changes may add on to this influence in any capacity. Overexpression of the enzyme Stearoyl CoA desaturase 1 (SCD1) in Chinese Hamster Ovary…

Murine leukemia-inducing retroviruses (MuLVs) can undergo changes that allow them to cause neurological diseases in rodents, and understanding the molecular basis for these changes will give us insights into the genetic elements required for distinct disease phenotypes. We have been studying PVC-211 MuLV, a variant of the erythroleukemia-inducing Friend MuLV…

Force spectroscopy of prestin, the motor protein in the inner ear. Credit: Kanazawa University As mammals, we have very sensitive hearing, thanks to a process known as cochlear amplification. This amplification is down to sensory cells in the inner ear called outer hair cells (OHCs), and it is thought to…

Overview Low glucose cell cultivation (eg. ～1 g/L) for biopharmaceutical production requires frequent sampling and frequent glucose supplementation. The excessive sampling requirement not only reduces the volume of the culture medium, but also is an unnecessary burden on the operators when performed manually. Here, we demonstrated that the Advanced Control…

By Zhang Linyu, Xiao Hui, and Wang Xuebin, Corning Incorporated, Corning Life Sciences Asia Technology Center Mammalian cells are a well-established system for the expression of biologically active recombinant proteins and antibodies that possess appropriate post-translational modifications.1 Stable expression technologies have yielded kilograms of protein from mammalian cells.2 However, some…

BACKGROUND: Exposure of Chinese hamster ovary (CHO) cells to fractionated x irradiation in vitro has led to expression of a distinctive multiple-drug-resistant phenotype. This phenotype is characterized by overexpression of P-glycoprotein without an increase in P-glycoprotein messenger RNA or gene amplification; increased glutathione S-transferase (GST) activity; and resistance to vincristine,…

1. Van den Berge K, Hembach KM, Soneson C, Tiberi S, Clement L, Love MI, Patro R, Robinson MD. RNA sequencing data: Hitchhikers guide to expression analysis. Annu Rev Biomed Data Sci. 2019;2(1):139–73. doi.org/10.1146/annurev-biodatasci-072018-021255. Article  Google Scholar  2. Conesa A, Madrigal P, Tarazona S, Gomez-Cabrero D, Cervera A, McPherson A,…

1. Eyun, S. Phylogenomic analysis of Copepoda (Arthropoda, Crustacea) reveals unexpected similarities with earlier proposed morphological phylogenies. BMC Evol. Biol. 17, 23 (2017). PubMed  PubMed Central  Google Scholar  2. Eyun, S. et al. Evolutionary history of chemosensory-related gene families across the Arthropoda. Mol. Biol. Evol. 34, 1838–1862 (2017). CAS  PubMed …

doi.org/10.1016/S0960-0760(03)00250-4Get rights and content Abstract Most cultured cell lines require addition of serum to the medium to maintain their proliferative capacity. For studies examining the cellular effects of estrogens serum is charcoal-stripped to remove steroids. Nonetheless, addition of the selective estrogen receptor modulator (SERM) 4-hydroxytamoxifen (4-OHT) inhibits the basal transcriptional…

Available online 17 December 2021 doi.org/10.1016/j.ymben.2021.12.004Get rights and content Highlights • Combined transcription factor engineering (BLIMP1 and XBP1s) reprogrammes CHO cell phenotype towards that of professional secretory cells. • Combinatorial transcription factor action generates additive responses (protein synthetic, secretory capacity, metabolic efficiency) towards a desirable phenotype. • Resultant new host…

NEW YORK – Sphere Fluidics is planning to use a recent fundraising round to build out new applications for its Cyto-Mine single-cell isolation and assay platform, with an eye toward gaining new customers in drug discovery and development. “There’s about 25 different things you’d like to prove in biopharma discovery,”…

doi.org/10.1016/0304-3835(80)90107-XGet rights and content Abstract The induction and repair of alkali labile lesions and single strand breaks in DNA of CHO cells following HpD photoradiation or X-irradiation has been examined. For treatments producing equal survival levels, the amount of DNA damage was less for HpD-photoradiated cells compared to X-irradiated cells….

Available online 9 December 2021 doi.org/10.1016/j.jbiotec.2021.12.003Get rights and content Highlights • First model for multi-amino acid multi-type-transporter system in CHO cells • Analytical expression for estimating amino acid uptake rates from concentration data • Predicts experimentally reported uptake rate from data not used in model formulation • Predicts change in…

Introduction Invaded by exogenous or endogenous pathogens, the host immune system will be activated accordingly to resist harm and maintain homeostasis, which includes innate immunity and adaptive immunity. As the first line of host immune defense, innate immunity plays a critical role in recognizing extracellular and intracellular pathogens (1, 2)….

Plain Language Summary Fluoropyrimidine (FP; 5-FU, capecitabine) is a common chemotherapy used to treat many different cancers, including cancer of the colon and rectum, upper digestive tract, breast and head and neck. Cancer patients who receive FP chemotherapy are at risk of developing severe side effects from their treatment. A…

This repository is the official implementation of “Relational Self-Attention: What’s Missing in Attention for Video Understanding” by Manjin Kim*, Heeseung Kwon*, Chunyu Wang, Suha Kwak, and Minsu Cho (*equal contribution). Requirements Python: 3.7.9 Pytorch: 1.6.0 TorchVision: 0.2.1 Cuda: 10.1 Conda environment environment.yml To install requirements: conda env create -f environment.yml…

install pytorch in jetson nano Done! Getting Started with Jetson Nano In this tutorial, you will learn how to set up the NVIDIA ® Jetson ™ Nano and install everything you need to use the full power of the tiny embedded board. Git – Version Contol…

Introduction Intestinal ischemia-reperfusion (II/R) injury is a severe clinical complication common in the Intensive Care Unit (ICU). It is associated with high morbidity and mortality.1 Usually, this problem is followed by various causes, including sepsis, shock, trauma, and so on.2 Intestinal ischemia-reperfusion injury destroys intestinal tissue and impairs the function…

Possible biomarkers for prognosis and clinical outcomes were identified, through the use whole exome sequencing and RNA sequencing, as therapeutic targets for patients with pancreatic ductal adenocarcinoma (PDAC), according to a recent study. The study, published in Therapeutic Advances in Medical Oncology, also reconfirmed genomic landscapes, major driver mutations, and…

1. Hackstein JH, Stumm CK. Methane production in terrestrial arthropods. Proc Natl Acad Sci USA. 1994;91:5441–5. CAS  PubMed  PubMed Central  Article  Google Scholar  2. Hackstein JHP, van Alen TA. Fecal methanogens and vertebrate evolution. Evolution. 1996;50:559–72. PubMed  Article  PubMed Central  Google Scholar  3. Borrel G, McCann A, Deane J, Neto…

Witawat Tunyong,1 Weewan Arsheewa,2 Sirijan Santajit,3,4 Thida Kong-ngoen,1 Pornpan Pumirat,1 Nitat Sookrung,5,6 Wanpen Chaicumpa,6 Nitaya Indrawattana1 1Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand; 2Department of Microbiology, Phrapokklao Hospital, Chanthaburi, 22000, Thailand; 3School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat, 80161, Thailand;…

Introduction Nuclear protein in testis (NUT) carcinoma (NC) is defined by the rearrangement of the chromosomal region 15q14 harboring the NUTM1 gene. As a clinically aggressive neoplasm with poor differentiation, NC was previously believed to occur primarily in children and young adolescents. However, with an increasing number of reports, middle-aged…

Tsuneaki Kenzaka,1,2 Yuto Shinkura,1 Shizuo Kayama,3– 5 Liansheng Yu,3– 5 Sayoko Kawakami,3 Motoyuki Sugai,3– 5 Satoru Kawasaki1 1Department of Internal Medicine, Hyogo Prefectural Tamba Medical Center, Tanba, Japan; 2Division of Community Medicine and Career Development, Kobe University Graduate School of Medicine, Kobe, Japan; 3Antimicrobial Resistance Research Center, National Institute of…