Tag: CHO

Photos Event Description Women in AI Myanmar, WaiMasterClass DataScience Series Event-2[Kaggle for Data Scientists] by Aye Hninn Khine and Cho Zin Tun Welcome from WaiMasterClass 2022 by Women in AI Myanmar! Women in AI Myanmar is hosting WaiMasterClass DataScience Series in 2022. There will be a total of 4 classes…

Barclay, A.N.: Membrane proteins with immunoglobulin-like domains–a master superfamily of interaction molecules. Semin Immunol. 15(4), 215–223 (2003) Article  CAS  Google Scholar  Bornhofft, K.F., Goldammer, T., Rebl, A., Galuska, S.P.: Siglecs: a journey through the evolution of sialic acid-binding immunoglobulin-type lectins. Dev. Comp. Immunol. 86, 219–231 (2018). doi.org/10.1016/j.dci.2018.05.008 Article  CAS  Google…

A. Miranda-Filho, J. Lortet-Tieulent, F. Bray, B. Cao, S. Franceschi, S. Vaccarella and L. Dal Maso, Lancet Diabetes Endocrinol., 9(4), 225 (2021) Article  Google Scholar  J. Ferlay, M. Colombet, I. Soerjomataram, D. M. Parkin, M. Piñeros, A. Znaor and F. Bray, Int. J. Cancer, Online ahead of print (2021). B….

Post-translational modifications (PTMs)—the chemical changes that proteins undergo following biosynthesis—account for most protein forms, or proteoforms. Indeed, according to conservative estimates, there are 1 million proteoforms, 90% of which are believed to be PTM-derived proteoforms. Estimates for the number of proteoforms and the percentage of PTM-derived proteoforms sometimes range quite…

Until now, identification of single cells after seeding has meant waiting for days/weeks until colonies grow and working backwards through the daily images in cell line development. For limiting dilution experiments, it’s only after those days/weeks have elapsed that issues may become visible, for example if no colonies grow due…

doi: 10.1002/biot.202200570. Online ahead of print. Affiliations Expand Affiliations 1 Department of Biological Sciences and Bioengineering, Inha University. 2 Division of Biological Science and Technology, Yonsei University. Item in Clipboard Ji Hwan Lee et al. Biotechnol J. 2023. Show details Display options Display options Format AbstractPubMedPMID doi: 10.1002/biot.202200570. Online ahead of…

Bailey LA, Hatton D, Field R, Dickson AJ (2012) Determination of Chinese hamster ovary cell line stability and recombinant antibody expression during long-term culture. Biotechnol Bioeng 109:2093–2103 CAS  Google Scholar  Barbosa MD (2011) Immunogenicity of biotherapeutics in the context of developing biosimilars and biobetters. Drug Discov Today 16:345–353 CAS  Google…

Belliveau J, Papoutsakis ET (2022) Extracellular vesicles facilitate large-scale dynamic exchange of proteins and RNA among cultured Chinese hamster ovary and human cells. Biotechnol Bioeng 119(5):1222–1238. doi.org/10.1002/bit.28053 Article  CAS  Google Scholar  Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle…

Title Simultaneous expression of ClopHensor and SLC26A3 reveals the nature of endogenous oxalate transport in CHO cells. Abstract ClopHensor, a fluorescent fusion protein, is a dual function biosensor that has been utilized as a tool for the simultaneous measurement of intracellular chloride and pH in cells. ClopHensor has traditionally been…

European Molecular Biology Laboratory, European Bioinformatics Institute, Hinxton, UK Claudia Calabrese, Nuno A. Fonseca, Lara Urban, Claudia Calabrese, Nuno A. Fonseca, Lara Urban, Liliana Greger, Nuno A. Fonseca, Lara Urban, Claudia Calabrese, Liliana Greger, Roland F. Schwarz, Oliver Stegle, Alvis Brazma, Alvis Brazma, Roland F. Schwarz & Oliver Stegle ETH Zurich, Zurich, Switzerland Natalie R. Davidson, André Kahles, Kjong-Van Lehmann, Natalie R. Davidson, André Kahles, Kjong-Van Lehmann, Stefan G. Stark, André Kahles, Kjong-Van Lehmann, Natalie R….

Image Credit: Shutterstock These are exciting times for the cell line development (CLD) market. An ever-increasing need for biologics to treat oncological, autoimmune, and infectious diseases is leading companies to seek more efficient and effective CLD support. This growing demand for biologics – primarily bispecific and mAbs, recombinant proteins, and…

Applied Tumor Genomics Research Program, Research Programs Unit, University of Helsinki, Helsinki, Finland Lauri A. Aaltonen Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, UK Federico Abascal, David J. Adams, Ludmil B. Alexandrov, Sam Behjati, Shriram G. Bhosle, David T. Bowen, Adam P. Butler, Peter J. Campbell, Peter Clapham, Helen Davies, Kevin J. Dawson, Stefan C. Dentro, Serge Serge, Erik Garrison, Mohammed Ghori, Dominik Glodzik, Jonathan Hinton, David…

The Broad Institute of MIT and Harvard, Cambridge, MA, USA Esther Rheinbay, Jeremiah A. Wala, Ofer Shapira, Grace Tiao, Julian M. Hess, Ziao Lin, Jaegil Kim, Yosef E. Maruvka, Pratiti Bandopadhayay, John Busanovich, Nicholas J. Haradhvala, Kiran Kumar, Gordon Saksena, Steven E. Schumacher, Chip Stewart, Manolis Kellis, Michael S. Lawrence, Pratiti Bandopadhayay, Rameen Beroukhim, Gad Getz, Nicholas J. Haradhvala, Julian M. Hess, Manolis Kellis, Jaegil Kim, Michael S. Lawrence, Ziao Lin, Yosef E. Maruvka, Esther Rheinbay, Gordon Saksena, Steven…

Jan 24 2023 DNA can signal the presence of or predisposition to a slew of diseases, including cancer. The ability to flag down these clues, known as biomarkers, allows medical professionals to make critical early diagnoses and provide personalized treatments. The typical methods of screening can be laborious, expensive or…

COPDGene Phase 3: Grant Support and Disclaimer: The project described was supported by Award Number U01 HL089897 and Award Number U01 HL089856 from the National Heart, Lung, and Blood Institute. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National…

Broadcast Date: March 8, 2023Time: 8:00 am PT, 11:00 am ET, 17:00 CET Since the first approval of a recombinant biopharmaceutical in 1986, Chinese hamster ovary (CHO) cells have become the industry workhorse for biologics production. While there have been dramatic improvements in multiple areas of cell line development (e.g., transfection and…

Developing a platform process to accelerate the development of other therapeutic proteins The production of recombinant therapeutic proteins requires the use of a host organism. The host organism that are commonly used for therapeutic proteins include CHO, E.coli, P. Pastoris, and S. Cerevisiae (1). The popularity of CHO for mammalian cell-based production is mainly…

Answer: Explain how making human tissue plasminogen activator (t-PA) in Chinese hamster ovary (CHO) cells is an example of genetic engineering? To produce t-PA for therapeutic use, researchers genetically engineered mammalian cell sussing Chinese hamster ovary cells. Explanation: Explain how making human tissue plasminogen activator (t-PA) in Chinese hamster ovary…

The Position Department Summary Many modern therapeutics are antibodies derived from mammalian cell culture, primarily Chinese Hamster Ovary (CHO) cell lines. Optimizing these cell lines for antibody production presents a clear path towards improving output and therefore reducing costs for these life-changing therapies. The candidate will join a collaboration of…

The Shodhganga@INFLIBNET Centre provides a platform for research students to deposit their Ph.D. theses and make it available to the entire scholarly community in open access. Please use this identifier to cite or link to this item: hdl.handle.net/10603/127172 Title:  Adaptation of recombinant human Erythropoietin rhEPO producing adherent Chinese Hamster Ovary…

Ai H, Fang X, Yang B, Huang Z, Chen H, Mao L, Zhang F, Zhang L, Cui L, He W, Yang J, Yao X, Zhou L, Han L, Li J, Sun S, Xie X, Lai B, Su Y, Lu Y, Yang H, Huang T, Deng W, Nielsen R, Ren J,…

2020 Alzheimer’s disease facts and figures. Alzheimers Dement 2020. Mayeda ER, Glymour MM, Quesenberry CP, Whitmer RA. Inequalities in dementia incidence between six racial and ethnic groups over 14 years. Alzheimers Dement. 2016;12:216–24. Article  Google Scholar  Matthews KA, Xu W, Gaglioti AH, Holt JB, Croft JB, Mack D, et al….

Biochim Biophys Acta Mol Cell Biol Lipids . 2022 Nov 29;159259. doi: 10.1016/j.bbalip.2022.159259. Online ahead of print. Affiliations Expand Affiliations 1 Graduate School of Technology, Industrial and Social Sciences, Tokushima University, Tokushima 770-8502, Japan; Graduate School of Biomedical Sciences, Tokushima University, Tokushima 770-8505, Japan. 2 Graduate School of Technology, Industrial…

WHO. World Malaria Report. Geneva: World Health Organization; 2019. Google Scholar  Rogerson SJ, Hviid L, Duffy PE, Leke RF, Taylor DW. Malaria in pregnancy: pathogenesis and immunity. Lancet Infect Dis. 2007;7:105–17. Article  CAS  PubMed  Google Scholar  Salanti A, Dahlback M, Turner L, Nielsen MA, Barfod L, Magistrado P, et al….

We have an exciting contract opportunity for a Bioinformatics analyst to support a collaboration between the Biologics Science and Technology group and Genomics Research Center, in which we are using genomics tools to optimize the development of better Chinese Hamster Ovary (CHO) cells for biologics manufacturing. The ideal candidate will…

Job Description To discuss more about this job opportunity, please reach out to Chitrank Rastogi (LinkedIn URL – www.linkedin.com/in/chitrank-rastogi-55119a102/), email your updated resume at chitrank.rastogi@collabera.com or give me a call at (425) 523-1648. Thank you! Job Description:Job Roles & Responsibilities: We have an exciting contract opportunity for a Bioinformatics analyst…

Description du poste Bioinformatics Analyst Supports a collaboration between the Biologics Science and Technology group and Genomics Research Center, in which we are using genomics tools to optimize the development of better Chinese Hamster Ovary (CHO) cells for biologics manufacturing. The ideal candidate will have broad experience in genomics data…

Cells and viruses hACE2 expressing BHK-21 (BHK-21-hACE2) and Vero-E6 cells were provided by State Key Laboratory of Virology, Wuhan University. Vero-E6 cells were cultured in DMEM (Gibco, Cat.11995–040) supplemented with 10% fetal bovine serum (Gibco, Cat.10091–148) and 1% penicillin and streptomycin solution (BasalMedia, Cat.S110JV) at 37°C with 5% CO2. BHK-21-hACE2…

02 November 2022 Goodwin Procter LLP To print this article, all you need is to be registered or login on Mondaq.com. Biogen seeks a preliminary injunction in its BPCIA case against Sandoz related to Sandoz’s proposed biosimilar of TYSABRI (natalizumab). On October 20, 2022, the Court…

Woolhouse MEJ, Gowtage-Sequeria S. Host range and emerging and reemerging pathogens. Emerg Infect Dis. 2005;11:1842–7. Article  PubMed  PubMed Central  Google Scholar  Allen T, Murray KA, Zambrana-Torrelio C, Morse SS, Rondinini C, Di Marco M, et al. Global hotspots and correlates of emerging zoonotic diseases. Nat Commun. 2017;8:1–10. Article  CAS  Google…

The data resource includes curated and unified data tables from 14 different human gut (feces) microbiome-metabolome published studies from recent years (Table 1, Supplementary Table 1)8,9,10,13,14,15,16,17,18,19,20,21,22,23. Figure 1a highlights the main data sources and key processing steps. For each study we provide 4 processed tables: A genus-level abundance table, a…

International Diabetes Federation (IDF). Diabetes Atlas 8th Edition 2017. www.idf.org/our-network/regions-members/africa/welcome.html. Accessed 15 July 2018 (2018). Singh, S., Usman, K. & Banerjee, M. Pharmacogenetic studies update in type 2 diabetes mellitus. World J. Diabetes. 7, 302. doi.org/10.4239/wjd.v7.i15.302 (2016). Article  PubMed  PubMed Central  Google Scholar  Inzucchi, S. E. et al. Management of…

The most recent report published by Market Research Inc. indicates that the Microbial Fermentation Technology Market is likely to accelerate significantly in the next few years. Specialists have studied market drivers, restraints, risks and prospects in the global market. The Microbial Fermentation Technology Market report shows the likely direction of…

Niikawa N, Matsuura N, Fukushima Y, Ohsawa T, Kajii T. Kabuki make-up syndrome: a syndrome of mentalretardation, unusual facies, large and protruding ears, and postnatal growth deficiency. J Pediatrics. 1981;99:565–9. CAS  Article  Google Scholar  Kuroki Y, Suzuki Y, Chyo H, Hata A, Matsui I. A new malformation syndrome of long…

Dyadic International (NASDAQ: DYAI) Next Generation Proteins for World Health C1 Vaccine Technology Presentation Mark Emalfarb, Founder & CEO Vaccine Technology Summit September 15-16, 2022 London UK Safe Harbor Regarding Forward-looking Statements Certain statements contained in this presentation are forward-looking statements within…

Visscher, P. M. et al. 10 years of GWAS discovery: biology, function, and translation. Am. J. Hum. Genet. 101, 5–22 (2017). CAS  PubMed  PubMed Central  Article  Google Scholar  Hekselman, I. & Yeger-Lotem, E. Mechanisms of tissue and cell-type specificity in heritable traits and diseases. Nat. Rev. Genet. 21, 137–150 (2020)….

Keywords Diffuse large B-cell lymphoma, Breast, Prognostic factors, Relapse, Central nervous system References [1] C. Wiseman, and K.T. Liao, Primary lymphoma of the breast. Cancer 29 (1972) 1705-12.www.ncbi.nlm.nih.gov/pubmed/4555557 [2] P.J. Hosein, J.C. Maragulia, M.P. Salzberg, O.W. Press, T.M. Habermann, J.M. Vose, M. Bast, R.H. Advani, R. Tibshirani, A.M. Evens, N….

Frontiers | Model-based intensification of CHO cell cultures: One-step strategy from fed-batch to perfusion …

Division of Cardiovascular Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, US Usman A. Tahir, Daniel H. Katz, Jeremy M. Robbins, Zsu-Zsu Chen, Mark D. Benson, Daniel E. Cruz, Debby Ngo, Shuliang Deng, Xu Shi, Shuning Zheng, Aaron S. Eisman, Laurie Farrell, James G. Wilson & Robert E. Gerszten Broad Institute of Harvard and MIT, Cambridge, MA, US Julian Avila-Pachecho, Alexander G….

Abstract Between 2014 and 2018, the global market for therapeutic monoclonal antibodies (mAbs) rose from $60 billion to $115.2 billion with a projected value of $300 billion by 2025. These molecules are used to effectively treat some of the most challenging illnesses from auto-immune diseases to cancer. While mAbs are…

Heffernan, A.L., Hare, D.J.: Tracing environmental exposure from neurodevelopment to neurodegeneration. Trends Neurosci. 41, 496–501 (2018) CAS  PubMed  Article  Google Scholar  Pacitti, D., Privolizzi, R., Bax, B.E.: Organs to cells and cells to organoids: the evolution of in vitro central nervous system modelling. Front Cell Neurosci. 13, 129 (2019) CAS …

1Department of Pulmonary and Critical Care Medicine, Sinopharm Tongmei General Hospital, Datong, People’s Republic of China; 2China-Japan Friendship Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People’s Republic of China; 3Department of Emergency, Chinese PLA General Hospital -Fourth Medical Center, Beijing, People’s Republic of China; 4School…

Salje, J. Cells within cells: Rickettsiales and the obligate intracellular bacterial lifestyle. Nat. Rev. Microbiol. 19, 375–390 (2021). CAS  PubMed  Article  Google Scholar  Wang, S. & Luo, H. Dating Alphaproteobacteria evolution with eukaryotic fossils. Nat. Commun. 12, 3324 (2021). CAS  PubMed  PubMed Central  Article  Google Scholar  Strassert, J. F. H.,…

Industry adoption of plant-made biologics remains slow, but plant-based technology gains an advantage by mainstream exposure. While Chinese hamster ovary (CHO) cells are the workhorse of biologics production, other cell expression systems are starting to gain ground, such as plant-based expression systems. Though an older technology in and of itself,…

doi: 10.3389/fbioe.2022.840600. eCollection 2022. Affiliations Expand Affiliations 1 School of Basic Medicine, Xinxiang Medical University, Xinxiang, China. 2 International Joint Research Laboratory for Recombinant Pharmaceutical Protein Expression System of Henan, Xinxiang Medical University, Xinxiang, China. Free PMC article Item in Clipboard Qin Li et al. Front Bioeng Biotechnol. 2022. Free PMC…

Introduction Tramadol (brand names ConZip, Ultram, UltramER, Odolo) is an analgesic used to treat moderate to severe pain. It is used for a variety of pain conditions, including post-operative pain, cancer pain, and musculoskeletal pain. Tramadol is a centrally acting opioid analgesic with mu-opioid binding activity as well as weak…

Introduction Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer, with an increasing global incidence, and a leading cause of cancer-related mortality.1 Although hepatitis B and C virus infections, cirrhosis, alcohol intake, and non-alcoholic fatty liver disease (NAFLD) are the main risk factors contributing to HCC.1,2 Approximately…

Hyperosmolality can occur during industrial fed-batch cultivation processes of Chinese hamster ovary (CHO) cells as highly concentrated feed and base solutions are added to replenish nutrients and regulate pH values. Some effects of hyperosmolality, such as increased cell size and growth inhibition, have been elucidated by previous research, but the…

Bispecific antibodies: rising stars in antibody therapeutics Monoclonal antibodies (mAbs) are important therapeutic agents for the treatment of many human diseases, such as cancer, autoimmune diseases, cardiovascular diseases, asthma, and viral infections. Unlike monospecific mAbs, bispecific antibodies (bsAbs) are antibodies containing two antigen-binding sites and therefore can simultaneously target two…

Introduction Breast cancer (BC) is the most commonly diagnosed cancer in women, and it is the main cause of cancer mortality in women around the world.1 Although standard treatments for breast cancer, including surgery, radiotherapy, chemotherapy, endocrine therapy, targeted therapy, and immunotherapy, have greatly improved during the last few decades.2,3…

Aguiar TQ, Santos SB, Martins IM, Domingues L, Oliveira C (2019) Production and bioengineering of recombinant pharmaceuticals. Proteins: sustainable source, processing and applications. Elsevier, Amsterdam, pp 259–293 Chapter  Google Scholar  Amadi IM, Agrawal V, Christianson T, Bardliving C, Shamlou P, LeBowitz JH (2020) Inhibition of endogenous miR-23a/miR-377 in CHO cells…

(PDF) Differential protein expression following low temperature culture of suspension CHO-K1 cells | Niraj Kumar – Academia.edu Academia.edu uses cookies to personalize content, tailor ads and improve the user experience. By using our site, you agree to our collection of information through the use of cookies. To learn more, view…

Experimental workflow and vector designs One way to validate the all-in-one reporter system is through transient or stable transfection of mammalian cells. Within this framework, the schematics in Fig. 1 illustrate the overall workflow of this study and feature the characteristics of promoters. To summarize, the study involves the engineering and…

Recombinant human erythropoietin (rHuEPO) is one of the biopharmaceuticals that dominates the market due to the presence of large number of patients suffering from anemia associated with end-stage renal disease, cancer or AIDS. EPO is a glycoprotein mainly produced by kidney in adults and liver in fetus, involved in the…

Introduction Antibody-drug conjugates (ADCs) have been studied for decades as a promising cancer treatment. As compared to conventional chemotherapy, ADCs have a larger therapeutic window because of their ability to target antigen-expressing tumor cells.1 As ADC drug development technology continues to evolve, several ADC drugs are being introduced to the…

Fassio F, Facioni MS, Guagnini F. Lactose maldigestion, malabsorption, and intolerance: a comprehensive review with a focus on current management and future perspectives. Nutrients. 2018. doi.org/10.3390/nu10111599. Article  PubMed  PubMed Central  Google Scholar  Gibson PR, Newnham E, Barrett JS, et al. Review article: fructose malabsorption and the bigger picture. Aliment Pharmacol…

David Gemmell looks at key unit operations, and why chemical engineers are heavily involved in their design, troubleshooting or optimisation TO DATE, the Covid-19 pandemic has taken the lives of over 5.7m1 people and impacted global trade, travel, and the livelihoods of billions. It also sparked a vaccine…

Abstract Chinese hamster ovary (CHO) cell is the predominant cell factory to produce biopharmaceuticals such as immunoglobulin G (IgG), but in CHO cells lactate accumulation and cell death compromise IgG production while fucosylation mitigates the antibody-dependent cellular cytotoxicity (ADCC) of IgG. To tackle these problems, we harnessed a burgeoning gene…

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed Version 1 : Received: 28 March 2022 / Approved: 28 March 2022 / Online: 28 March 2022 (10:11:34 CEST) Li, G.; Suzuki, H.; Asano, T.; Tanaka, T.; Suzuki, H.; Kaneko, M.K.; Kato, Y. Development of A Novel Anti-Epcam…

Chinese hamster ovary (CHO) cells are the most commonly used host cell lines for therapeutic protein production. Exposure of these cells to highly concentrated feed solution during fed-batch cultivation can lead to a non-physiological increase in osmolality (> 300 mOsm/kg) that affects cell physiology, morphology, and proteome. As addressed in…

Chinese Hamster Ovary (CHO) cells are a popular workhorse for production of recombinant therapeutic proteins. Amenable to both adherent and suspension culture, CHO cells offer versatility in growth platform that is favorable to the end user’s requirements. So why the switch? Simply put, growing CHO cells in suspension will allow…

BioCyc ID:TU00094 Synonyms: OP00133 Local Context (not to scale — see Genome Browser for correct scale) Transcription Unit Notes: Click on a binding site (if any) to navigate to the page for its transcription factor. Click on a gene to navigate to its gene page. Key to regulator colors: Green:…

Blog Agriculture Biotechnology Though CRISPR-Cas enabled targeted genome engineering across a vast array of organisms, the system features major disadvantages. Frequent off-target mutagenesis, licensing restrictions and non-ideal economic license terms have inhibited commercial crop-science product upscaling, and, in many cases, entirely disqualified CRISPR’s use by many commercial crop developers. Seeking…

1. Introduction The approval of Chinese hamster ovary (CHO) cell cultures for the manufacturing of protein therapeutic products modernized conventional medicine. CHO cells remained the workhorse for the production of monoclonal antibodies (mAbs) for the last at least 20 years for different reasons. First, CHO cells are easily adapting and…

Introduction Primary testicular diffuse large B-cell lymphoma (PT-DLBCL) is a rare and aggressive form of mature B-cell lymphoma.1–3 PT-DLBCL was the most common type of testicular tumor in men aged over 60 and characterized by painless uni- or bilateral testicular masses with infrequent constitutional symptoms.4–6 PT-DLBCL shows significant extranodal tropism,…

John Wikswo, founder and director of the Vanderbilt Institute for Integrative Biosystems Research and Education and Gordon A. Cain University Professor, is the principal investigator of a $1 million award from the National Science Foundation. The object is to build a pathbreaking “robot scientist”—a fully automated microfluidic system for parallel,…

Introduction Hair graying is one of the representative signs of aging. It has been considered to be triggered by a decreased number of follicular melanocyte stem cells (MeSCs; Nishimura et al., 2005; Iida et al., 2020) or dysfunction of MeSCs such as decreased oxidation resistance capacity with aging (Shi et…

Introduction Rotator cuff tear (RCT) is a common shoulder disorder causing shoulder pain and disability. The prevalence of full-thickness RCT is 20.7% in the general population, and increased with age.1 Rotator cuff play essential roles in shoulder function and the treatment of proximal humeral fractures.2,3 It is important to repair…

1.) What is the theoretical yield (in moles) of aluminum chloride… – 133.34 g/mol) if 0.385 mol of aluminum are reacted with 0.800 mol of chlorine. (g) —> 2AlCl(s)   2.) A sample of impure potassium chloride weighing 0.4260 grams is dissolved in water and treated with an excess of silver…

Computer translation Trying to learn how to translate from the human translation examples. English chinese hamster ovary cells Human contributions From professional translators, enterprises, web pages and freely available translation repositories. Add a translation English chinese hamster ovary cell Last Update: 2014-12-09 Usage Frequency: 1 Quality: English chinese hamster ovary…

doi.org/10.1016/0360-3016(89)90304-0Get rights and content Abstract A cell line, CHO-Chlr, which has elevated levels of glutathione-S-transferase (GST), is resistant to damage caused by bi-functional nitrogen mustards. This cell line has a similar radiation sensitivity to and value of oxygen enhancement ratio as the wild type cells, CHO-KI, from which the mutant…

doi.org/10.1016/0014-4827(83)90345-2Get rights and content Abstract The ultrastructural localization of small nuclear RNA (snRNA) was studied by EM autoradiography in Chinese Hamster Ovary (CHO) cells. Conditions were set where most (>85 %) of the nuclear [3H]uridine label consisted of snRNA, the most abundant species being U1, U2 and the nucleolar species…

Aghamohseni H, Ohadi K, Spearman M, Krahn N, Moo-Young M, Scharer JM, Butler M, Budman HM (2014) Effects of nutrient levels and average culture pH on the glycosylation pattern of camelid-humanized monoclonal antibody. J Biotechnol 186:98–109. doi.org/10.1016/j.jbiotec.2014.05.024 CAS  Article  PubMed  Google Scholar  Altamirano C, Illanes A, Casablancas A, Gámez X,…

Welcome from WaiMasterClass 2022 by Women in AI Myanmar! Women in AI Myanmar is hosting WaiMasterClass DataScience Series in 2022. There will be a total of 4 classes for this WaiMasterClass DataScience Series. This is our second event in Datascience series. In event 2, Aye Hninn Khine and Cho Zin…

Aggarwal RS (2014) What’s fueling the biotech engine-2012 to 2013. Nat Biotechnol 32:32–39. doi.org/10.1038/nbt.2794 CAS  Article  PubMed  Google Scholar  Becker DJ, Lowe JB (2003) Fucose: biosynthesis and biological function in mammals. Glycobiology 13:41–53. doi.org/10.1093/glycob/cwg054 CAS  Article  Google Scholar  Chan KF, Shahreel W, Wan C et al (2016) Inactivation of GDP-fucose…

Engineering of lipid-metabolism in CHO cells by over-expression of SCD1AbstractIntroduction–Overall aim is to investigate how SCD1 over-expression influences target recombinant protein expression, as well as how temperature changes may add on to this influence in any capacity. Overexpression of the enzyme Stearoyl CoA desaturase 1 (SCD1) in Chinese Hamster Ovary…

Murine leukemia-inducing retroviruses (MuLVs) can undergo changes that allow them to cause neurological diseases in rodents, and understanding the molecular basis for these changes will give us insights into the genetic elements required for distinct disease phenotypes. We have been studying PVC-211 MuLV, a variant of the erythroleukemia-inducing Friend MuLV…

Force spectroscopy of prestin, the motor protein in the inner ear. Credit: Kanazawa University As mammals, we have very sensitive hearing, thanks to a process known as cochlear amplification. This amplification is down to sensory cells in the inner ear called outer hair cells (OHCs), and it is thought to…

Overview Low glucose cell cultivation (eg. ～1 g/L) for biopharmaceutical production requires frequent sampling and frequent glucose supplementation. The excessive sampling requirement not only reduces the volume of the culture medium, but also is an unnecessary burden on the operators when performed manually. Here, we demonstrated that the Advanced Control…

By Zhang Linyu, Xiao Hui, and Wang Xuebin, Corning Incorporated, Corning Life Sciences Asia Technology Center Mammalian cells are a well-established system for the expression of biologically active recombinant proteins and antibodies that possess appropriate post-translational modifications.1 Stable expression technologies have yielded kilograms of protein from mammalian cells.2 However, some…

BACKGROUND: Exposure of Chinese hamster ovary (CHO) cells to fractionated x irradiation in vitro has led to expression of a distinctive multiple-drug-resistant phenotype. This phenotype is characterized by overexpression of P-glycoprotein without an increase in P-glycoprotein messenger RNA or gene amplification; increased glutathione S-transferase (GST) activity; and resistance to vincristine,…

1. Van den Berge K, Hembach KM, Soneson C, Tiberi S, Clement L, Love MI, Patro R, Robinson MD. RNA sequencing data: Hitchhikers guide to expression analysis. Annu Rev Biomed Data Sci. 2019;2(1):139–73. doi.org/10.1146/annurev-biodatasci-072018-021255. Article  Google Scholar  2. Conesa A, Madrigal P, Tarazona S, Gomez-Cabrero D, Cervera A, McPherson A,…

1. Eyun, S. Phylogenomic analysis of Copepoda (Arthropoda, Crustacea) reveals unexpected similarities with earlier proposed morphological phylogenies. BMC Evol. Biol. 17, 23 (2017). PubMed  PubMed Central  Google Scholar  2. Eyun, S. et al. Evolutionary history of chemosensory-related gene families across the Arthropoda. Mol. Biol. Evol. 34, 1838–1862 (2017). CAS  PubMed …

doi.org/10.1016/S0960-0760(03)00250-4Get rights and content Abstract Most cultured cell lines require addition of serum to the medium to maintain their proliferative capacity. For studies examining the cellular effects of estrogens serum is charcoal-stripped to remove steroids. Nonetheless, addition of the selective estrogen receptor modulator (SERM) 4-hydroxytamoxifen (4-OHT) inhibits the basal transcriptional…

Available online 17 December 2021 doi.org/10.1016/j.ymben.2021.12.004Get rights and content Highlights • Combined transcription factor engineering (BLIMP1 and XBP1s) reprogrammes CHO cell phenotype towards that of professional secretory cells. • Combinatorial transcription factor action generates additive responses (protein synthetic, secretory capacity, metabolic efficiency) towards a desirable phenotype. • Resultant new host…

NEW YORK – Sphere Fluidics is planning to use a recent fundraising round to build out new applications for its Cyto-Mine single-cell isolation and assay platform, with an eye toward gaining new customers in drug discovery and development. “There’s about 25 different things you’d like to prove in biopharma discovery,”…

doi.org/10.1016/0304-3835(80)90107-XGet rights and content Abstract The induction and repair of alkali labile lesions and single strand breaks in DNA of CHO cells following HpD photoradiation or X-irradiation has been examined. For treatments producing equal survival levels, the amount of DNA damage was less for HpD-photoradiated cells compared to X-irradiated cells….

Available online 9 December 2021 doi.org/10.1016/j.jbiotec.2021.12.003Get rights and content Highlights • First model for multi-amino acid multi-type-transporter system in CHO cells • Analytical expression for estimating amino acid uptake rates from concentration data • Predicts experimentally reported uptake rate from data not used in model formulation • Predicts change in…

Introduction Invaded by exogenous or endogenous pathogens, the host immune system will be activated accordingly to resist harm and maintain homeostasis, which includes innate immunity and adaptive immunity. As the first line of host immune defense, innate immunity plays a critical role in recognizing extracellular and intracellular pathogens (1, 2)….

Plain Language Summary Fluoropyrimidine (FP; 5-FU, capecitabine) is a common chemotherapy used to treat many different cancers, including cancer of the colon and rectum, upper digestive tract, breast and head and neck. Cancer patients who receive FP chemotherapy are at risk of developing severe side effects from their treatment. A…

This repository is the official implementation of “Relational Self-Attention: What’s Missing in Attention for Video Understanding” by Manjin Kim*, Heeseung Kwon*, Chunyu Wang, Suha Kwak, and Minsu Cho (*equal contribution). Requirements Python: 3.7.9 Pytorch: 1.6.0 TorchVision: 0.2.1 Cuda: 10.1 Conda environment environment.yml To install requirements: conda env create -f environment.yml…

install pytorch in jetson nano Done! Getting Started with Jetson Nano In this tutorial, you will learn how to set up the NVIDIA ® Jetson ™ Nano and install everything you need to use the full power of the tiny embedded board. Git – Version Contol…

Introduction Intestinal ischemia-reperfusion (II/R) injury is a severe clinical complication common in the Intensive Care Unit (ICU). It is associated with high morbidity and mortality.1 Usually, this problem is followed by various causes, including sepsis, shock, trauma, and so on.2 Intestinal ischemia-reperfusion injury destroys intestinal tissue and impairs the function…

Possible biomarkers for prognosis and clinical outcomes were identified, through the use whole exome sequencing and RNA sequencing, as therapeutic targets for patients with pancreatic ductal adenocarcinoma (PDAC), according to a recent study. The study, published in Therapeutic Advances in Medical Oncology, also reconfirmed genomic landscapes, major driver mutations, and…

1. Hackstein JH, Stumm CK. Methane production in terrestrial arthropods. Proc Natl Acad Sci USA. 1994;91:5441–5. CAS  PubMed  PubMed Central  Article  Google Scholar  2. Hackstein JHP, van Alen TA. Fecal methanogens and vertebrate evolution. Evolution. 1996;50:559–72. PubMed  Article  PubMed Central  Google Scholar  3. Borrel G, McCann A, Deane J, Neto…

Witawat Tunyong,1 Weewan Arsheewa,2 Sirijan Santajit,3,4 Thida Kong-ngoen,1 Pornpan Pumirat,1 Nitat Sookrung,5,6 Wanpen Chaicumpa,6 Nitaya Indrawattana1 1Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand; 2Department of Microbiology, Phrapokklao Hospital, Chanthaburi, 22000, Thailand; 3School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat, 80161, Thailand;…

Introduction Nuclear protein in testis (NUT) carcinoma (NC) is defined by the rearrangement of the chromosomal region 15q14 harboring the NUTM1 gene. As a clinically aggressive neoplasm with poor differentiation, NC was previously believed to occur primarily in children and young adolescents. However, with an increasing number of reports, middle-aged…

Tsuneaki Kenzaka,1,2 Yuto Shinkura,1 Shizuo Kayama,3– 5 Liansheng Yu,3– 5 Sayoko Kawakami,3 Motoyuki Sugai,3– 5 Satoru Kawasaki1 1Department of Internal Medicine, Hyogo Prefectural Tamba Medical Center, Tanba, Japan; 2Division of Community Medicine and Career Development, Kobe University Graduate School of Medicine, Kobe, Japan; 3Antimicrobial Resistance Research Center, National Institute of…