Categories
Tag: CHO
Sperm-specific histone H1 in highly condensed sperm nucleus of Sargassum horneri
Cho, C. et al. Haploinsufficiency of protamine-1 or-2 causes infertility in mice. Nat. Genet. 28, 82–86 (2001). Article CAS PubMed Google Scholar Oliva, R. Protamines and male infertility. Hum. Reprod. Update 12, 417–435 (2006). Article CAS PubMed Google Scholar Balhorn, R. The protamine family of sperm nuclear proteins. Genome Biol….
Sutro Biopharma Inc at Wells Fargo Healthcare Conference Transcr
Sep 09, 2022 / 02:25PM GMTJames John Shin – Wells Fargo Securities, LLC, Research Division – Associate Equity Analyst Good morning, everyone. Thanks for being here. My name is James Shin. I’m one of the biotech analyst at Wells Fargo. Today, I’m joined up here with the CEO of Sutro…
N6-methyladenosine modification positively regulate Japanese encephalitis virus replication | Virology Journal
Burgess HM, Depledge DP, Thompson L, Srinivas KP, Grande RC, Vink EI, Abebe JS, Blackaby WP, Hendrick A, Albertella MR, Kouzarides T, Stapleford KA, Wilson AC, Mohr I. Targeting the m6A RNA modification pathway blocks SARS-CoV-2 and HCoV-OC43 replication. Genes Dev. 2021;35:1005–19. Article CAS PubMed PubMed Central Google Scholar Chambers…
Comparison of capture-based mtDNA sequencing performance between MGI and illumina sequencing platforms in various sample types | BMC Genomics
Hu T, Chitnis N, Monos D, Dinh A. Next-generation sequencing technologies: an overview. Hum Immunol. 2021;82(11):801–11. Article CAS Google Scholar Jeon SA, Park JL, Park SJ, Kim JH, Goh SH, Han JY, Kim SY. Comparison between MGI and Illumina sequencing platforms for whole genome sequencing. Genes Genomics. 2021;43(7):713–24. Article CAS …
Proinflammatory chemokine CXCL14 activates MAS-related G protein-coupled receptor MRGPRX2 and its putative mouse ortholog MRGPRB2
Reagents and peptides CXC motif chemokine 14 (CXCL14) was purchased from BIOZOL (Eching, Germany). Proadrenomedullin N-terminal 20 peptide (PAMP-20) was obtained from Tocris Bioscience (Bristol, UK), and cortistatin-14 was from GenScript (NJ, USA). Custom synthesis of CXCL14 analogs was performed by JPT Peptide Technologies (Berlin, Germany) (see Supplementary Fig. 6). Stock…
Enhancing Human Glycoprotein Hormones Production in CHO Cells Using Heterologous Beta-Chain Signal Peptides
Jackson, R.C. and Blobel, G., Post-translational processing of full-length presecretory proteins with canine pancreatic signal peptidase, Ann. N.Y. Acad. Sci., 1980, vol. 343, pp. 391–404. Article PubMed CAS Google Scholar Weintraub, B.D., Stannard, B.S., Linnekin, D., and Marshall, M., Relationship of glycosylation to de novo thyroid-stimulating hormone biosynthesis and secretion…
Exploring the Latest Advances in Transcriptomics
Since the idea of genetic regulation was first postulated back in the 1950s, our scientific understanding of the transcriptome has deepened greatly.1 Looking into the transcriptome of cells and tissues has helped scientists to understand the biological processes that drive both health and disease; however, the complex and occasionally mysterious…
NOSTER’s latest solutions for comprehensive gut bacteria-based metabolite and microbiota analysis
KYOTO, Japan, Dec. 19, 2023 /PRNewswire/ — Japan’s Noster Inc., a premier provider of gut microbiome analysis services, is proud to unveil groundbreaking updates in its research on cutting-edge gut bacteria-based metabolite and gut microbiota analysis services. These advancements have far-reaching implications for research on human health and diseases such as…
Evidence that human Fc gamma receptor IIA (CD32) subtypes are not receptors for oxidized LDL
Several lines of evidence suggest that clearance of oxidized LDL (oxLDL) immune complexes by macrophage IgG Fc receptors (Fc gamma Rs) plays a role in atherogenesis. Ox-LDL may also be cleared directly by Fc gamma Rs, as shown for murine Fc gamma RII-B2. In humans, the homologous Fc gamma R…
Gadolinium Oxide Nanoparticles reinforce immune response
Boyi Yu,1– 4 Xuanyi Lu,5 Xianglong Feng,1– 4 Ting Zhao,1– 4 Jiaxin Li,1– 4 Yudie Lu,5 Fei Ye,1– 4 Xiongxiong Liu,1– 4 Xiaogang Zheng,1– 4 Zheyu Shen,5 Xiaodong Jin,1– 4 Weiqiang Chen,1– 4 Qiang Li1– 4 1Biomedical Center, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, People’s Republic of…
Precision Medicine: A New Era in Cancer Therapy
Precision medicine can offer improved clinical outcomes by assessing the unique characteristics of tumors, such as their likelihood of developing resistance to chemotherapy. Stay up to date on the latest science with Brush Up Summaries. Precision Medicine in Cancer Precision medicine for cancer treatment involves tailoring treatments to an individual…
Improving recombinant protein production in CHO cells using the CRISPR-Cas system
Kalkan, Ali Kerem; Palaz, Fahreddin; Sofija, Semeniuk; Elmousa, Nada; Ledezma, Yuri; Cachat, Elise; Rios-Solis, Leonardo; (2023) Improving recombinant protein production in CHO cells using the CRISPR-Cas system. Biotechnology Advances , 64 , Article 108115. 10.1016/j.biotechadv.2023.108115. Text Improving recombinant protein production in CHO cells using the CRISPR-Cas system.pdf – Accepted Version…
CD CHO Media Market Size Reveals the Best Marketing Channels In Global Industry | by Evalynkoepp | Dec, 2023
CD CHO Media Market Trends, Growth Opportunities, and Forecast Scenarios The CD CHO media market is experiencing significant growth and is expected to continue expanding in the coming years. CD CHO media, also known as chemically-defined cell culture media for Chinese hamster ovary (CHO) cells, is widely used in biopharmaceutical…
Maternal dominance contributes to subgenome differentiation in allopolyploid fishes
Otto, S. P. & Whitton, J. Polyploid incidence and evolution. Annu. Rev. Genet. 34, 401–437 (2000). Article CAS PubMed Google Scholar Van de Peer, Y., Maere, S. & Meyer, A. The evolutionary significance of ancient genome duplications. Nat. Rev. Genet. 10, 725–732 (2009). Article PubMed Google Scholar Comai, L. The…
CHO Cells Genetic Engineering | CreateDebate
Welcome to CreateDebate CreateDebate is a social debate community built around ideas, discussion and democracy. If this is your first time checking out a debate, here are some quick tips to help get you started: Arguments with the highest score are displayed first. Argument replies (both in favor and in…
Identification of Differentially Expressed Genes in Human Colorectal Cancer Using RNASeq Data Validated on the Molecular Level with Real-Time PCR
Allam RM, Al-Abd AM, Khedr A, Sharaf OA, Nofal SM, Khalifa AE, Mosli HA, Abdel-Naim AB (2018) Fingolimod interrupts the cross talk between estrogen metabolism and sphingolipid metabolism within prostate cancer cells. Toxicol Lett 291:77–85 Article CAS PubMed Google Scholar Andrews S et al (2010) FastQC: a quality control tool…
PULMOZYME (Dornase Alfa) Drug Insights and Market
Dublin, Dec. 14, 2023 (GLOBE NEWSWIRE) — The “PULMOZYME Drug Insight and Market Forecast – 2032” report has been added to ResearchAndMarkets.com‘s offering. This report provides comprehensive insights about PULMOZYME for Cystic Fibrosis in the six major markets. A detailed picture of the PULMOZYME for cystic fibrosis in the 7MM,…
Importance of a detailed anomaly scan after a cfDNA test indicating fetal trisomy 21, 18 or 13
Kagan KO, Sonek J, Kozlowski P (2022) Antenatal screening for chromosomal abnormalities. Arch Gynecol Obstet 305(4):825–835 Article CAS PubMed PubMed Central Google Scholar Rose NC, Barrie ES, Malinowski J, Jenkins GP, McClain MR, LaGrave D et al (2022) Systematic evidence-based review: the application of noninvasive prenatal screening using cell-free DNA…
A laboratory ice machine as a cold oligotrophic artificial microbial niche for biodiscovery
Flemming, H.-C. & Wuertz, S. Bacteria and archaea on earth and their abundance in biofilms. Nat. Rev. Microbiol. 17, 247–260 (2019). Article CAS PubMed Google Scholar Flemming, H.-C., Neu, T. R. & Wozniak, D. J. The EPS matrix: The “house of biofilm cells”. J. Bacteriol. 189, 7945–7947 (2007). Article CAS …
Comprehensive Review of Small Interfering RNAs (siRNAs)
Introduction Cancer, also called malignancy, is a group of diseases caused by the rapid multiplication and spread of malignant cells.1,2 According to statistics from the National Cancer Center, more than 100 types of cancer have been identified.3 The ability of malignant tumor cells to metastasize via the blood-lymphatic system and…
CD CHO Media Market Research Report Unlocks Analysis on the Market Financial Status, Market Size, and Market Revenue upto 2030
CD CHO Media Market Analysis and Latest Trends CD CHO Media is a specialized cell culture medium used for the growth and production of CHO (Chinese Hamster Ovary) cells in biopharmaceutical manufacturing processes. CHO cells are widely used in the production of therapeutic proteins, vaccines, and monoclonal antibodies. CD CHO…
Transition of allele-specific DNA hydroxymethylation at regulatory loci is associated with phenotypic variation in monozygotic twins discordant for psychiatric disorders | BMC Medicine
Lichtenstein P, Yip BH, Bjork C, Pawitan Y, Cannon TD, Sullivan PF, Hultman CM. Common genetic determinants of schizophrenia and bipolar disorder in Swedish families: a population-based study. Lancet. 2009;373(9659):234–9. Article PubMed Google Scholar Maurano MT, Humbert R, Rynes E, Thurman RE, Haugen E, Wang H, Reynolds AP, Sandstrom R,…
Predicting productivity in CHO cells based on co-occurrence of metabolism-related features in simultaneous label-free autofluorescence multiharmonic (SLAM) microscopy
Efficient cell line development is crucial for optimizing biopharmaceutical production. We demonstrate the potential of SLAM and FLIM microscopy to optimize this process by correlating metabolism-related features with measured productivity in early CHO cell passages. Eight CHO cell lines were imaged using SLAM and FLIM microscopy, and a pipeline was…
RNA Aptamer-Mediated Gene Activation Systems for Inducible Transgene Expression in Animal Cells
journal contribution posted on 2023-12-11, 05:47 authored by Feiyang Zheng, Yoshinori Kawabe, Masamichi Kamihira RNA expression analyses can be used to obtain various information from inside cells, such as physical conditions, the chemical environment, and endogenous signals. For detecting RNA, the system regulating intracellular gene expression has the potential for…
The Helicobacter pylori Genome Project: insights into H. pylori population structure from analysis of a worldwide collection of complete genomes
Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden Kaisa Thorell Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Chihuahua, Chihuahua, México Zilia Y. Muñoz-Ramírez Cancer Genomics Research Laboratory, Frederick National Laboratory for Cancer Research, Frederick, MD, USA Difei Wang, Yunhu Wan, Belynda Hicks, Bin Zhu, Meredith Yeager, Amy Hutchinson, Kedest Teshome, Kristie Jones & Wen Luo…
Odronextamab Continues to Appear Safe, Effective in Patients With R/R DLBCL
Treatment with odronextamab (REGN1979) led to an objective response rate (ORR) of 52%, with encouraging progression-free (PFS) and overall survival (OS) among complete responders in a cohort of patients with relapsed/refractory diffuse large B-cell lymphoma (DLBCL), according to a final analysis of the phase 2 ELM-2 trial (NCT03888105) presented at…
Mammalian Cell Expression System Market is booming in the globe by 2029 |Thermo Fisher Scientific, Bio Basic, GenScript Biotech, Oxford Expression Technologies, ProteoGenix
[New York, November 2023] — An insightful market analysis report focusing on the Mammalian Cell Expression System Market has just been released by Stats N Data, serving as a valuable resource for both industry leaders and newcomers. This comprehensive report offers a detailed exploration of the global Mammalian Cell Expression…
Employment of a high throughput functional assay to define the critical factors that influence vaccine induced cross-variant neutralizing antibodies for SARS-CoV-2
Study design, ethical statement, and sample collection The vaccinated participants were recruited under the COVID-19 PROTECT study (2012/00917). The convalescent plasma samples were collected from subjects who were diagnosed with COVID-19 by positive polymerase chain reaction (PCR) results (study 2020/00120). All participants provided written informed consent in accordance with the…
Human Recombinant CCL24 (from CHO cells)
1. Validity The present general terms of sale and delivery apply to all business of VWR International GmbH, i.e. particularly to industrial chemicals, reagents and diagnostic products as well as general laboratory supplies including equipment for the use of these products and consulting. By placing an order the customer irrevocably…
Why CHO than yeast For production of insulin?
What are the causes of insolubility of insulin in E coli during protein expression?5 answersInsolubility of insulin in E. coli during protein expression can be caused by several factors. One factor is the formation of inclusion bodies, which are aggregates of misfolded proteins that accumulate in the cytoplasm of E….
Why are HEK293 cells used for transfection?
What is one example of ongoing gene therapy can be used in keratoplasty?5 answersGene therapy in ophthalmology has shown potential for use in keratoplasty. The cornea is an ideal target for gene therapy due to its accessibility, low volume, and special immunological status. Studies have focused on improving gene transfer…
HDA signs Land 400 Phase 3 contract
Hanwha Defense Australia (HDA) has signed an approximately $4 billion contract with the Commonwealth for the delivery of 129 Redback Infantry Fighting Vehicles (IFVs). With a down select of HDA as the preferred partner for the Land 400 Phase 3 project announced in July 2023, the program will see all…
High-throughput ligand profile characterization in novel cell lines expressing seven heterologous insect olfactory receptors for the detection of volatile plant biomarkers
Generation of the stable OR cell lines In vivo measured ligand profile data derived from the DoOR database (neuro.uni-konstanz.de/DoOR/default.html)19,20 were used to select 12 ORs, which can be appropriate to detect the pathogen-related VOCs relevant for our study. To assemble a novel cell-based olfactory panel, 11 stable cell lines were…
r – ggplot2 shapes superimposed even when specified directly
I’m trying to make a simple graph where one variable is shape and the other is fill in ggplot2. I’ve tried grouping the samples by different variables, and I’ve tried manually specifying the shapes for each variable, but every time I get this superimposed image where each sample has many…
CHO Cell Culture Media Market Size, Market Share and Global Market Analysis Report, 2023
CHO Cell Culture Media Market Analysis and Latest Trends CHO (Chinese hamster ovary) cell culture media is a widely used medium for the production of therapeutics, vaccines, and recombinant proteins. CHO cells are commonly used in biopharmaceutical research and development due to their ability to produce complex proteins with post-translational…
Identification and validation of key miRNAs for colon cancer
Introduction With nearly 2 million new cases and 1 million deaths worldwide in 2020, colorectal cancer is the third-most common cancer and the second leading cause of cancer-related deaths.1 According to data from the US Surveillance, Epidemiology and End Results program and the National Program of Cancer Registries program, the…
tRNA therapeutics for genetic diseases
Huang, X. et al. The landscape of mRNA nanomedicine. Nat. Med. 28, 2273–2287 (2022). Article CAS Google Scholar Rohner, E., Yang, R., Foo, K. S., Goedel, A. & Chien, K. R. Unlocking the promise of mRNA therapeutics. Nat. Biotechnol. 40, 1586–1600 (2022). Article CAS Google Scholar Brown, A., Shao, S.,…
Antibody Production Companies: Re-shaping Life Sciences Together
(Photo : Michal Jarmoluk from Pixabay) Antibody production companies are essential in dealing with the growing demand for antibodies for life sciences, therapeutic, and diagnostic applications. Therefore, a number of service providers are specialized in offering various types of services regarding the complex production of antibodies. Many biotechnology companies rely…
Targeting the epigenome to reinvigorate T cells for cancer immunotherapy | Military Medical Research
Tsui C, Kretschmer L, Rapelius S, Gabriel SS, Chisanga D, Knöpper K, et al. MYB orchestrates T cell exhaustion and response to checkpoint inhibition. Nature. 2022;609(7926):354–60. Article CAS PubMed PubMed Central Google Scholar Zhu L, Zhou X, Gu M, Kim J, Li Y, Ko CJ, et al. Dapl1 controls NFATc2…
The Frequency of CYP2D6 and CYP3A4/5 Genotypes and The Impact of Their Allele Translation and Phenoconversion-Predicted Enzyme Activity on Risperidone Pharmacokinetics in Saudi Children with Autism
Al-Dosari MS, Al-Jenoobi FI, Alkharfy KM, Alghamdi AM, Bagulb KM, Parvez MK, Al-Mohizea AM, Al-Muhsen S, Halwani R (2013) High prevalence of CYP2D6*41 (G2988A) allele in Saudi Arabians. Environ Toxicol Pharmacol 36:1063–1067. doi.org/10.1016/j.etap.2013.09.008 Article PubMed CAS Google Scholar Almandil NB, Alkuroud DN, AbdulAzeez S, AlSulaiman A, Elaissari A, Borgio JF…
Heterologous Protein Production in Cho Cells: Methods and Protocols (Methods in Molecular Biology #1603) (Hardcover)
$233.99 Usually Ships in 1-5 Days Description This volume discusses protocols that cover genetic manipulation of Chinese hamster ovary (CHO) cells for recombinant protein production, and protocols for the characterization of CHO cells using ‘omic approaches. This book also explores methods that discuss the genome editing tool, CRISPR/Cas9, and the…
Dissecting cell death pathways in fed-batch bioreactors
doi: 10.1002/biot.202300257. Online ahead of print. David A Mentlak 1 , John Raven 2 , Tessa Moses 3 , Fraser Massie 3 , Nicholas Barber 2 , Robyn Hoare 2 , Graeme Burton 2 , Alison Young 2 , Leon P Pybus 2 , Susan Rosser 4 , Robert J White 1 , Daniel Ungar 1 , Nia J Bryant 1 Affiliations…
Two mitochondrial HMG-box proteins, Cim1 and Abf2, antagonistically regulate mtDNA copy number in Saccharomyces cerevisiae | Nucleic Acids Research
Abstract The mitochondrial genome, mtDNA, is present in multiple copies in cells and encodes essential subunits of oxidative phosphorylation complexes. mtDNA levels have to change in response to metabolic demands and copy number alterations are implicated in various diseases. The mitochondrial HMG-box proteins Abf2 in yeast and TFAM in mammals…
Solved A monoclonal antibody is produced using a genetically
A monoclonal antibody is produced using a genetically modified CHO cell culture in a bioreactor of 5m3 volume that is operated at 37\deg C. The CHO cells have an oxygen requirement of 3 x 10–10 mmol per hour and per number of cells. The critical oxygen requirement is 0.015 mM….
Identification and characterization of ARID1A-interacting proteins in renal tubular cells and their molecular regulation of angiogenesis | Journal of Translational Medicine
Centore RC, Sandoval GJ, Soares LMM, Kadoch C, Chan HM. Mammalian SWI/SNF chromatin remodeling complexes: emerging mechanisms and therapeutic strategies. Trends Genet. 2020;36:936–50. Article CAS PubMed Google Scholar Pagliaroli L, Trizzino M. The Evolutionary conserved SWI/SNF subunits ARID1A and ARID1B Are Key modulators of pluripotency and cell-fate determination. Front Cell…
Transformer-based tool recommendation system in Galaxy | BMC Bioinformatics
Kumar A, Rasche H, Grüning B, Backofen R. Tool recommender system in Galaxy using deep learning. GigaScience. 2021. doi.org/10.1093/gigascience/giaa152. Article PubMed PubMed Central Google Scholar The galaxy community: the galaxy platform for accessible, reproducible and collaborative biomedical analyses: 2022 update. Nucleic Acids Res 50(W1):W345-W35104 2022. (2022). doi.org/10.1093/nar/gkac247 Gil Y, Ratnakar…
Exploring the promising potential of induced pluripotent stem cells in cancer research and therapy | Molecular Cancer
Rowe RG, Daley GQ. Induced pluripotent stem cells in disease modelling and drug discovery. Nat Rev Genet. 2019;20:377–88. Article CAS PubMed PubMed Central Google Scholar Li L, Papadopoulos V. Advances in stem cell research for the treatment of primary hypogonadism. Nat Rev Urol. 2021;18:487–507. Article CAS PubMed Google Scholar Lawrence…
Potential threats, promising resources in thriving colonies of bacteria and fungi on ocean plastic trash
(L-R) Members of the NTU research team include Jonas Koh, NTU doctoral student, Singapore Centre for Environmental Life Sciences Engineering (SCELSE); Zin Thida Cho, School of Civil and Environmental Engineering (CEE), NTU; Dr Sakcham Bairoliya, Research Fellow, School of CEE, NTU. Credit: Nanyang Technological University A team of scientists from…
Phase 3 Trials Reveal New Standards of Care for NSCLC
Data from several recent phase 3 trials support changes to standard care for non-small cell lung cancer (NSCLC), according to researchers. Results from the MARIPOSA trial suggest that amivantamab plus lazertinib represents a new first-line standard of care for patients with advanced, EGFR-mutant NSCLC.1 The MARIPOSA-2 trial suggests that amivantamab…
Adipose cDC1s contribute to obesity-associated inflammation through STING-dependent IL-12 production
Ward, Z. J. et al. Projected U.S. state-level prevalence of adult obesity and severe obesity. N. Engl. J. Med. 381, 2440–2450 (2019). Article PubMed Google Scholar Khaodhiar, L., McCowen, K. C. & Blackburn, G. L. Obesity and its comorbid conditions. Clin. Cornerstone 2, 17–31 (1999). Article CAS PubMed Google Scholar …
Phylogenetic, population structure, and population demographic analyses reveal that Vicia sepium in Japan is native and not introduced
Vicia sepium L. (bush vetch, Fabaceae) is a small perennial herb that is widely distributed on the Eurasian continent in the temperate Euro-Siberian broad-leaved forest region1. It has a wide distribution, both horizontally and vertically; for example, in the Swiss Alps, it grows from the lowlands to the sub alpine…
Gene expression profiles separate endometriosis lesion subtypes and indicate a sensitivity of endometrioma to estrogen suppressive treatments through elevated ESR2 expression | BMC Medicine
Cea Soriano L, López-Garcia E, Schulze-Rath R, Garcia Rodríguez LA. Incidence, treatment and recurrence of endometriosis in a UK-based population analysis using data from The Health Improvement Network and the Hospital Episode Statistics database. Eur J Contracept Reprod Health Care. 2017;22(5):334–43. Article PubMed Google Scholar Guo SW. Recurrence of endometriosis…
Decline of DNA damage response along with myogenic differentiation
Introduction Proper functioning of all living organisms depends on the faithful maintenance and transmission of genomic information stored in the molecule of DNA. However, DNA integrity is continuously challenged by a variety of endogenous and exogenous agents causing DNA lesions which have a critical impact on cellular activities and homeostasis….
Fermentation | Free Full-Text | Whole-Genome Sequencing of Lactiplantibacillus plantarum YY-112 and Investigation of Its Immune-Modulating Abilities In Vivo
Author Contributions Conceptualization, Y.Y. and Y.G.; Data curation, M.L. and W.Z.; Formal analysis, M.L., W.Z., W.T. and J.L.; Funding acquisition, Y.Y. and Y.G.; Investigation, J.X., Y.Y. and Y.G.; Methodology, M.L., J.L. and Y.Y.; Project administration, Y.Y.; Resources, J.X., Y.Y. and Y.G.; Software, M.L., W.Z. and W.T.; Supervision, J.X., Y.Y. and…
synergistic strategies combining low-temperature culture and mTORC1 signaling engineering
Introduction: The growing demand for recombinant proteins in medicine has prompted biopharmaceutical companies to seek ways to maximize the manufacturing process. Despite its known negative impact on cell growth, temperature shift (TS) has emerged as a cost-effective strategy to enhance protein quantity and quality in Chinese Hamster Ovary cells (CHO)….
synergistic strategies combining low-temperature culture and mTORC1 signaling engineering.docx
Introduction: The growing demand for recombinant proteins in medicine has prompted biopharmaceutical companies to seek ways to maximize the manufacturing process. Despite its known negative impact on cell growth, temperature shift (TS) has emerged as a cost-effective strategy to enhance protein quantity and quality in Chinese Hamster Ovary cells (CHO)….
Phenotypic drug-susceptibility profiles and genetic analysis based on whole-genome sequencing of Mycobacterium avium complex isolates in Thailand
Abstract Mycobacterium avium complex (MAC) infections are a significant clinical challenge. Determining drug-susceptibility profiles and the genetic basis of drug resistance is crucial for guiding effective treatment strategies. This study aimed to determine the drug-susceptibility profiles of MAC clinical isolates and to investigate the genetic basis conferring drug resistance using…
Fully instrumented 3D printed small-scale bioreactor developed
To support upstream bioprocess development, a study has demonstrated real-time monitoring of the growth phase in a 3D printed, single-use bioreactor. Researchers have reported on the development of a small-scale 3D printed, single-use bioreactor suitable for a broad range of mammalian and microbial cultivation. Overall, the study described in a…
Taxonomic identification and temperature stress tolerance mechanisms of Aequorivita marisscotiae sp. nov
Zain ul Arifeen, M., Ma, Y.-N., Xue, Y.-R. & Liu, C.-H. Deep-sea fungi could be the new arsenal for bioactive molecules. Mar. Drugs 18, 9 (2020). Article Google Scholar Martínez-Espinosa, R. M. Microorganisms and their metabolic capabilities in the context of the biogeochemical nitrogen cycle at extreme environments. Int. J….
Herpes Zoster Pipeline Analysis Demonstrates Novel 18+
Herpes Zoster Pipeline DelveInsight’s, “Herpes Zoster Pipeline Insight 2023,” report provides comprehensive insights about 18+ companies and 20+ pipeline drugs in the Herpes Zoster pipeline landscape. It covers the Herpes Zoster pipeline drug profiles, including Herpes Zoster clinical trials and nonclinical stage products. It also covers the therapeutics assessment by…
Intrinsic deletion at 10q23.31, including the PTEN gene locus, is aggravated upon CRISPR-Cas9-mediated genome engineering in HAP1 cells mimicking cancer profiles
Introduction The CRISPR-Cas system is a widely used genome engineering technology because of its simple programmability, versatile scalability, and targeting efficiency (Wang & Doudna, 2023). Although researchers are rapidly developing CRISPR-Cas9 tools, the biggest challenge remains to overcome undesired on- and off-targeting outcomes. Previous studies have reported unintended genomic alterations,…
Molecular detection and whole genome sequencing of Pectobacterium and Dickeya, the casual agents of soft rot disease in lettuce
Azadmanesh S, Marefat A (2013) and Kayhan Azadmanesh. Detection of Pectobacteria causal agents of potato soft rot in north-western provinces of Iran. J Plant Pathol Microbiol 4(1) Babadoost M (1990) Bacterial soft rot of vegetables, fruits and ornamentals. Rep Plant Dis 943:7 Google Scholar Ben Fekih I, Zhang C, Li…
Knockdown of GNL3L alleviates the progression of COPD
Introduction Chronic obstructive pulmonary disease (COPD) is a common chronic bronchitis disease characterized by persistent airflow limitation, which can be prevented and treated. COPD is the third leading cause of death in the world reported in 2020.1 With the progression of the disease, COPD will lead to respiratory failure, pulmonary…
Prediction of CHO cell line stability using expression of DNA repair genes
doi: 10.1002/biot.202300425. Online ahead of print. Affiliations Expand Affiliations 1 Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, Delaware, USA. 2 National Institute for Innovation in Manufacturing Biopharmaceuticals, Newark, Delaware, USA. 3 Pfizer, Inc, 875 Chesterfield Pkwy W, Chesterfield, Missouri, USA. Item in Clipboard Lauren T Cordova et al….
Advances in CHO Cell Line Development for Biotherapeutics
A diverse array of biological medicines – including therapeutic proteins, hormones, monoclonal antibodies, cytokines, enzymes and vaccines – are now used to prevent and treat a wide range of illnesses. While some of these biotherapeutics were originally obtained from natural origins, such as donated blood, many are now synthesized using…
What CHO cell excrete that make pH becomes increasingly alkaline?
How can DNA be exracted from ancient bone? 4 answers What are the tensile properties of bamboo? 5 answers What are the different methods for measuring glucose uptake in different tissues in humans? 5 answers How many culture day is suitable for transfected si-RNA into the mouse isolated keratinocytes? 3…
Electroporation Transformation of Barley | SpringerLink
Ahloowalia BS (1987) Plant regeneration from embryo-callus culture in barley. Euphytica 36: 659–665 CrossRef Google Scholar Ahokas H (1989) Transfection of germinating barley seed electrophoretically with exogenous DNA. Theor Appl Genet 77: 469–472 CrossRef CAS Google Scholar Barro F, Cannell ME, Lazzeri PA, Barcelo P (1998) The influence of auxins…
Predicting circRNA-disease association using heterogeneous network and meta-path
[1] H. L. Sanger, G. Klotz, D. Riesner, H. J. Gross, A. K. Kleinschmidt, Viroids are single-stranded covalently closed circular RNA molecules existing as highly base-paired rod-like structures, Proc. Natl. Acad. Sci. USA, 73 (1976), 3852–3856. doi.org/10.1073/pnas.73.11.3852 doi: 10.1073/pnas.73.11.3852 …
Oncogenic activation revealed by FGFR2 genetic alterations in intrahepatic cholangiocarcinomas | Cell & Bioscience
Everhart JE, Ruhl CE. Burden of digestive diseases in the United States Part III: Liver, biliary tract, and pancreas. Gastroenterology. 2009;136(4):1134–44. Article PubMed Google Scholar Khan SA, Thomas HC, Davidson BR, Taylor-Robinson SD. Cholangiocarcinoma. Lancet. 2005;366(9493):1303–14. Article PubMed Google Scholar Nakanuma Y, Klimstra DS, Komuta M, Zen Y (2019) Intrahepatic…
ABCB1 and immune genes in breast cancer
Introduction Chemoresistance is a major challenge for breast cancer treatment.1 The mechanisms of chemoresistance are complex because of crosstalk between receptor tyrosine kinases and downstream pathways, deregulation of cell-cycle and apoptosis regulators, and modulation of tumor-infiltrating immune cells.2 The ATP-binding cassette (ABC) superfamily is one of the largest families of…
What are the factors for instability of CHO cells in production processes?
What is the impact of patellofemoral pain syndrome on the quality of life of patients? 5 answers How to perform semi purification of flavonoids? 5 answers What is a good artefact to compare 3D optical scanners? 3 answers What is the genetic architecture of flowering time in the worldwide Arabidopsis…
What are the reasons for instability of CHO cells in production processes?
1.In what ways does having well-documented processes and standards benefit both franchisees and the franchisor? 4 answers What is the need of surface modification or coating for protein adsorption? 5 answers Is there any reagent taht can detect 2-hg in vivo? 5 answers What is the relationship between consumer trust…
Rising Use of CHO Cells in the Genetic Study Drives the North America Chinese Hamster Ovary (CHO) Cells Market Growth
The increasing use of CHO cells in genetic studies reflects their unique advantages in the field of genetic research. CHO cells are renowned for their genetic stability. Researchers study specific genes or genetic modifications without the concern of unexpected genetic changes interfering with their experiments. This stability is particularly crucial…
Enhancing Productivity of Chinese Hamster Ovary (CHO) Cells: Synergistic Strategies Combining Low-Temperature Culture and mTORC1 Signaling Engineering
1Golestan University of Medical Sciences, Iran 2Golestan University, Iran The final, formatted version of the article will be published soon. Notify me Receive an email when it is updated You just subscribed to receive the final version of the article Introduction:…
Genome interpretation in a federated learning context allows the multi-center exome-based risk prediction of Crohn’s disease patients
Goodwin, S., McPherson, J. D. & Richard McCombie, W. Coming of age: Ten years of next-generation sequencing technologies. Nat. Rev. Genet. 17(6), 333–351 (2016). Article CAS PubMed PubMed Central Google Scholar Van Dijk, E. L., Auger, H., Jaszczyszyn, Y. & Thermes, C. Ten years of next-generation sequencing technology. Trends Genet….
Characterization of intrinsic and effective fitness changes caused by temporarily fixed mutations in the SARS-CoV-2 spike E484 epitope and identification of an epistatic precondition for the evolution of E484A in variant Omicron | Virology Journal
Cele S, Gazy I, Jackson L, Hwa SH, Tegally H, Lustig G, et al. Escape of SARS-CoV-2 501Y.V2 from neutralization by convalescent plasma. Nature. 2021;593(7857):142–6. Article CAS PubMed PubMed Central Google Scholar Planas D, Bruel T, Grzelak L, Guivel-Benhassine F, Staropoli I, Porrot F, et al. Sensitivity of infectious SARS-CoV-2…
Engineered CHO cells as a novel AAV production platform for gene therapy delivery
The Herpes simplex virus (HSV)-based platform for production of recombinant adeno-associated viral vectors (rAAVs) yields higher titers and increased percentage of full capsids when compared to the triple transient transfection (TTT) method. However, this platform currently faces two major challenges. The first challenge is the reliance on commercial media, sometimes…
Who are the leading innovators in rDNA expression systems for the pharmaceutical industry?
However, not all innovations are equal and nor do they follow a constant upward trend. Instead, their evolution takes the form of an S-shaped curve that reflects their typical lifecycle from early emergence to accelerating adoption, before finally stabilizing and reaching maturity. Identifying where a particular innovation is on this…
Biologic Production and Host Cell Proteins
Chinese hamster ovary (CHO) cell lines have been a prominent choice to produce monoclonal antibodies (mAbs) and their derived counterparts, such as antibody–drug conjugates (ADCs), antibody fragments and Fc-fusion proteins. Currently, 89% of biologics produced in mammalian systems use the CHO cell platform.1 Over the past few decades, mAb yields…
CloneMedia CHO Growth A
Limiting dilution or fluorescence-activated cell sorting (FACS) is traditionally used to clone CHO cells. These methods can be inefficient as they may require additional rounds of sub-cloning to obtain a high probability of monoclonality. The CloneMedia CHO Growth A is a viscous semi-solid media that immobilizes single CHO cells, allowing…
An FcRn-targeted mucosal vaccine against SARS-CoV-2 infection and transmission
Mice and Golden Syrian hamsters All research experiments in this study comply with all relevant ethical regulations. The Institutional Animal Care and Use Committee approved the animal protocol at the University of Maryland (#R-APR-20-18 and #R-MAR-21-19). The animals were acclimatized at the animal facility for 4–6 days before initiating experiments….
It’s Time for CDMOs to Embrace the CRISPR Advantage
CDMOs are actively exploring and leveraging both new and existing technologies to streamline the cell-line development process at every step. The biotech and pharmaceutical pipeline is packed with experimental biological therapeutics such as antibodies, recombinant proteins, enzymes, and vaccines, which hold transformative potential for many health conditions. Taking these promising…
Molecular diagnosis, phylogenetic analysis, and antifungal susceptibility profiles of Candida species isolated from neutropenic oncological patients | BMC Infectious Diseases
Eissa S, Khedr R, Romeih M, Halaby L, Elanany M, Madney Y. Clinical characteristics and outcome of invasive fungal sinusitis in children with hematological malignancies. Med Mycol. 2022;60(4):myac010. Article PubMed Google Scholar Lin G-L, Chang H-H, Lu C-Y, Chen C-M, Lu M-Y, Lee P-I, Jou S-T, Yang Y-L, Huang L-M,…
Non-coding RNA-mediated endothelial-to-mesenchymal transition in human diabetic cardiomyopathy, potential regulation by DNA methylation | Cardiovascular Diabetology
Raghavan S, Vassy JL, Ho Y, Song RJ, Gagnon DR, Cho K, et al. Diabetes mellitus-related all-cause and cardiovascular mortality in a national cohort of adults. J Am Heart Assoc. 2019;8(4): e011295. Article PubMed PubMed Central Google Scholar Jia G, Whaley-Connell A, Sowers JR. Diabetic cardiomyopathy: a hyperglycaemia—and insulin-resistance-induced heart…
Stable Chinese Hamster Ovary Suspension Cell Lines Harboring Recombinant Human Cytochrome P450 Oxidoreductase and Human Cytochrome P450 Monooxygenases as Platform for In Vitro Biotransformation Studies
In the liver, phase-1 biotransformation of drugs and other xenobiotics is largely facilitated by enzyme complexes consisting of cytochrome P450 oxidoreductase (CPR) and cytochrome P450 monooxygenases (CYPs). Generated from human liver-derived cell lines, recombinant in vitro cell systems with overexpression of defined phase-1 enzymes are widely used for pharmacological and…
Simplified Single-Cell Isolation
Sponsored content brought to you by Many increasingly used applications, such as cell line engineering and sequencing (including ATAC-seq and RNA-seq) along with single-cell genomics, require single-cell isolation as a starting point. But the cost and complexity of cell sorters have traditionally relegated this technology to larger lab groups or…
Glycoengineered keratinocyte library reveals essential functions of specific glycans for all stages of HSV-1 infection
Generation of a glycogene knock out library in HaCaT keratinocytes HaCaT is a human keratinocyte cell line capable of forming a stratified squamous epithelium, and thus allows evaluating the infection of the skin tropic HSV-1 in both cell and organotypic tissue culture. In order to address the role of specific…
Exploring permittivity measurements versus viable cell counts
Cell culture is a complex and involved process. Its success relies on the balance of many important process parameters to provide ideal process conditions for optimal cell growth, maintenance, and target product production. Viable cell density (VCD) is a key performance indicator (KPI) of the effect of the critical process…
Elucidating the effects of microbubble pinch-off dynamics on mammalian cell viability
doi: 10.1002/bit.28582. Online ahead of print. Affiliations Expand Affiliations 1 Department of Mechanical Engineering, Boston University, Boston, Massachusetts, USA. 2 Department of Mechanical Engineering, Dunwoody College of Technology, Minneapolis, Minnesota, USA. 3 Amgen, Cambridge, Massachusetts, USA. 4 Global Processing Engineering, Biogen, Cambridge, Massachusetts, USA. Item in Clipboard Oliver McRae et al….
What are the scale-dependent factors that increases the level of high mannose in perfusion culture of CHO cells?
The level of high mannose in perfusion culture of CHO cells is influenced by scale-dependent factors. One factor is the concentration of manganese (Mn(2+)) in the culture medium, which has been shown to significantly affect the high mannose glycan content. Another factor is the availability of glucose in the culture…
IL-1R8 expression in DLBCL regulates NK cell recruitment and influences patient prognosis
Al-Mterin MA, Murshed K, Elkord E (2022) PD-1 expression, among other immune checkpoints, on tumor-infiltrating NK and NKT cells is associated with longer disease-free survival in treatment-naive CRC patients. [Journal Article]. Cancer Immunol Immunother. doi.org/10.1007/s00262-022-03337-8 Baecklund E, Smedby KE, Sutton LA, Askling J, Rosenquist R (2014) Lymphoma development in patients…
Why are Hek293 cells predominantly used in production of viral vectors compared to CHO
Hek293 cells are predominantly used in the production of viral vectors compared to CHO cells due to several reasons. Firstly, Hek293 cells have been found to be efficient in transducing different cell types and have been successfully used in gene and cell therapy protocols. Secondly, transcriptomic analysis has revealed that…
Reduction of retinal ganglion cell death in mouse models of familial dysautonomia using AAV-mediated gene therapy and splicing modulators
Animals All mice were housed in the AALAC-accredited Animal Resource Center at Montana State University. All animal use protocols were approved by the Montana State University Institutional Animal Care and Use Committee (IACUC) (protocol No. 2020-15-IA; Bozeman, MT). The study fulfilled the ARRIVE guidelines, and all experiments complied with relevant…
Methods for Scaling and Protein Purification
CHO cells are the predominant host for biotherapeutic protein expression, with roughly 70% of licensed biologics manufactured in CHO. Multiple attributes make CHO cells desirable for bioproduction, including the ability to adapt to high-density suspension culture in serum-free and chemically-defined media and the incorporation of post-translational modifications that are biologically…
[Solved] Describe a cell system that could be used to produce EPO Explain – Foundations of Biochemistry (BIOC192)
Erythropoietin (EPO) Production Erythropoietin (EPO) is a glycoprotein hormone that stimulates the production of red blood cells. It is primarily produced in the kidneys and is used therapeutically in the treatment of anemia related to chronic kidney disease and chemotherapy. Mammalian Cell Culture System One of the most common systems…
Design, optimization, production and activity testing of recombinant immunotoxins expressed in plants and plant cells for the treatment of monocytic leukemia
. 2023 Dec;14(1):2244235. doi: 10.1080/21655979.2023.2244235. Affiliations Expand Affiliations 1 Bioprocess Engineering, Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Aachen, Germany. 2 Institute for Molecular Biotechnology, RWTH Aachen University, Aachen, Germany. 3 University of Natural Resources and Life Sciences, Vienna (BOKU), Department of Biotechnology (DBT), Institute of Bioprocess Science…
Accelerating Bulk Harvest Testing Using The Blazar CHO Animal Origin Free Virus Panel For Rapid Adventitious Virus Detection
The biologics industry is continually developing new manufacturing approaches designed to improve yield, reduce processing time and costs, and harness the power of digitalization to increase process efficiency. In line with these advancements, regulators are publishing guidance on the use of new technologies for adventitious agent detection, which can support…
More than 2,200 students attend autumn open days at TUS Athlone campus
Students from Columba College at the TUS Athlone open day. Published: Thu 26 Oct 2023, 9:51 AM A record number of attendees visited the Technological University of the Shannon’s (TUS) Athlone campus for its 2023 undergraduate autumn open days. With more than 2,200 students registered to attend, in addition to…
ACF Supplements Market Poised to Achieve US$ 384.5 Billion by 2032 with a Strong CAGR of 8.9%
The worldwide ACF supplements market is expected to arrive at US$ 384.5 Billion by 2032. The market is developing quickly and is supposed to be esteemed at US$ 163.9 Billion in 2022 and will enlist at a huge CAGR of 8.9 % during the gauge time frame from 2022 to 2032. ACF…
Phase 3 Trial Supports Amivantamab Plus Lazertinib Over Osimertinib in Advanced EGFR+ NSCLC
Phase 3 Trial Supports Amivantamab Plus Lazertinib Over Osimertinib in Advanced EGFR+ NSCLC In the frontline setting, amivantamab-vmjw (Rybrevant) and lazertinib (Leclaza) outperformed osimertinib (Tagrisso) alone in the phase 3 MARIPOSA trial (NCT04487080) that were presented at the 2023 ESMO Congress.1 Patients with advanced non–small cell lung cancer (NSCLC) harboring classical EGFR sensitizing mutations, who received…