Tag: crispr

Specific High-Sensitivity Enzymatic Molecular Detection System Termed RPA-Based CRISPR-Cas13a for Duck Tembusu Virus Diagnostics

journal contribution posted on 19.05.2022, 07:04 by Dalin He, Gang Liu, Jing Yang, Xiaoning Jiang, Hongzhi Wang, Yaru Fan, Shangyu Gong, Feng Wei, Youxiang Diao, Yi Tang In China, drastic losses in the economy have been caused by the Tembusu virus (TMUV), the causative agent of the egg-drop syndrome, to…

Continue Reading Specific High-Sensitivity Enzymatic Molecular Detection System Termed RPA-Based CRISPR-Cas13a for Duck Tembusu Virus Diagnostics

In vivo hypermutation and continuous evolution

Arnold, F. H. Design by directed evolution. Acc. Chem. Res. 31, 125–131 (1998). Google Scholar  Packer, M. S. & Liu, D. R. Methods for the directed evolution of proteins. Nat. Rev. Genet. 16, 379–394 (2015). Google Scholar  Drake, J. W., Charlesworth, B., Charlesworth, D. & Crow, J. F. Rates of…

Continue Reading In vivo hypermutation and continuous evolution

CENTURY THERAPEUTICS, INC. Management’s discussion and analysis of financial condition and results of operations (form 10-Q)

The following discussion and analysis should be read in conjunction with our financial statements and accompanying notes included in this Quarterly Report on Form 10-Q and the financial statements and accompanying notes thereto for the fiscal year ended December 31, 2021 and the related Management’s Discussion and Analysis of Financial…

Continue Reading CENTURY THERAPEUTICS, INC. Management’s discussion and analysis of financial condition and results of operations (form 10-Q)

A Highly Sensitive and Specific Detection Method for Mycobacterium tuberculosis Fluoroquinolone Resistance Mutations Utilizing the CRISPR-Cas13a System

Objectives CRISPR-Cas13a system-based nucleic acid detection methods are reported to have rapid and sensitive DNA detection. However, the screening strategy for crRNAs that enables CRISPR-Cas13a single-base resolution DNA detection of human pathogens remains unclear. Methods A combined rational design and target mutation-anchoring CRISPR RNA (crRNA) screening strategy was proposed. Results…

Continue Reading A Highly Sensitive and Specific Detection Method for Mycobacterium tuberculosis Fluoroquinolone Resistance Mutations Utilizing the CRISPR-Cas13a System

WGS Facilitates Gene Editing System Upgrade

Researchers at the Korean Institute of Life Sciences and Technology engineered an efficient, miniaturized CRISPR-Cas gene-editing system that may be more easily packed into vectors for clinical applications. Their system employs the Cas variant Cas12f1 with a guide RNA (gRNA) remodeled to mitigate off-target effects, a design that could potentially…

Continue Reading WGS Facilitates Gene Editing System Upgrade

Novel supramolecular CRISPR-Cas9 carrier enables more efficient genome editing — ScienceDaily

Clustered regularly interspaced short palindromic repeats (CRISPR) and their accompanying protein, CRISPR-associated protein 9 (Cas9), made international headlines a few years ago as a game-changing genome editing system. Consisting of Cas9 and strand of genetic material known as a single-guide RNA (sgRNA), the system can target specific regions of DNA…

Continue Reading Novel supramolecular CRISPR-Cas9 carrier enables more efficient genome editing — ScienceDaily

Editas Medicine (EDIT) Falls 4.05% on May 10

Last Price $ Last Trade Change $ Change Percent % Open $ Prev Close $ High $ low $ 52 Week High $ 52 Week Low $ Market Cap PE Ratio Volume Exchange EDIT – Market Data & News Trade Editas Medicine Inc (NASDAQ: EDIT) has fallen $0.45 (4.05%) and …

Continue Reading Editas Medicine (EDIT) Falls 4.05% on May 10

CALITHERA BIOSCIENCES, INC. MANAGEMENT’S DISCUSSION AND ANALYSIS OF FINANCIAL CONDITION AND RESULTS OF OPERATIONS (form 10-Q)

You should read the following discussion and analysis of our financial condition and results of operations in conjunction with our unaudited condensed consolidated financial statements and related notes included in Part I, Item 1 of this report. This Quarterly Report on Form 10-Q contains forward-looking statements within the meaning of…

Continue Reading CALITHERA BIOSCIENCES, INC. MANAGEMENT’S DISCUSSION AND ANALYSIS OF FINANCIAL CONDITION AND RESULTS OF OPERATIONS (form 10-Q)

Global CRISPR/Cas9 Market In- Depth Research, Industry Statistics 2022

Global “CRISPR/Cas9 Market” Reports 2022 provide key industry studies for CRISPR/Cas9 manufacturers with specific statistics, significance, definition, SWOT analysis, expert opinion and the latest developments in the world. The research report also covers market size, price, sales, revenue, market shares, gross margin, growth rate and cost structure. The report aims…

Continue Reading Global CRISPR/Cas9 Market In- Depth Research, Industry Statistics 2022

Intellia (NTLA) Drops 4.76% on May 5

Last Price $ Last Trade Change $ Change Percent % Open $ Prev Close $ High $ low $ 52 Week High $ 52 Week Low $ Market Cap PE Ratio Volume Exchange NTLA – Market Data & News Trade Intellia Therapeutics Inc (NASDAQ: NTLA) shares are down 4.76%, or $2.65 per…

Continue Reading Intellia (NTLA) Drops 4.76% on May 5

ISPE announces 2022 Facility of the Year Awards winners

Winners of ISPE’s Facility of the Year Awards (FOYA) 2022 include Janssen, Takeda, CRISPR Therapeutics, Catalent and Iovance Biotherapeutics. ISPE has announced the 2022 Facility of the Year Awards (FOYA) Category Winners at the 2022 ISPE Europe Annual Conference in Madrid, Spain.  FOYA is the global awards programme recognising innovation and creativity…

Continue Reading ISPE announces 2022 Facility of the Year Awards winners

Identifying Mechanisms of Methylation in Stem Cells

Normal cells (red) survive much longer than Set1A-mutant cells (green) when ING5 is deleted. Northwestern Medicine scientists have identified critical regulatory processes that govern differentiation in embryonic stem cells, according to a study published in the Proceedings of the National Academy of the Sciences (PNAS). Bercin Cenik, MD, PhD, postdoctoral…

Continue Reading Identifying Mechanisms of Methylation in Stem Cells

New Products Posted to GenomeWeb: Thermo Fisher, Guardant Health, EmpowerDx, More

Thermo Fisher Invitrogen TrueTag Donor DNA Kits  Thermo Fisher Scientific has launched Invitrogen TrueTag Donor DNA kits for cell line engineering with CRISPR-Cas9 or TALEN technology. They allow researchers to enrich tagged cells and skip clonal isolation. The kits support four types of locus-specific knock-in templates and feature a one-step…

Continue Reading New Products Posted to GenomeWeb: Thermo Fisher, Guardant Health, EmpowerDx, More

Technical Officer / Micro-injectionist job with EMBL

The European Molecular Biology Laboratory (EMBL) is one of the highest-ranked scientific research organisations in the world. The Headquarters Laboratory is located in Heidelberg (Germany), with additional sites in Barcelona (Spain), Grenoble (France), Hamburg (Germany), Hinxton (UK), and Rome (Italy). The EMBL Epigenetics and Neurobiology Unit in Rome is supported…

Continue Reading Technical Officer / Micro-injectionist job with EMBL

Recent developments in miRNA based recombinant protein expression in CHO

Aguiar TQ, Santos SB, Martins IM, Domingues L, Oliveira C (2019) Production and bioengineering of recombinant pharmaceuticals. Proteins: sustainable source, processing and applications. Elsevier, Amsterdam, pp 259–293 Chapter  Google Scholar  Amadi IM, Agrawal V, Christianson T, Bardliving C, Shamlou P, LeBowitz JH (2020) Inhibition of endogenous miR-23a/miR-377 in CHO cells…

Continue Reading Recent developments in miRNA based recombinant protein expression in CHO

Plant Breeding and CRISPR Plants Market Trends 2022, Global Industry Share, Size, Global Industry Analysis, Key Market Growth Analysis, Segments, Emerging Technologies, Opportunity and Forecast 2027

The “Plant Breeding and CRISPR Plants Market” research report 2021-2027 offering the complete outline of market size, share and growth opportunities, industry segmentation by product type, applications and geographical representation. The report highlights product development along with technological upgrades that can boost the market growth. The Plant Breeding and CRISPR…

Continue Reading Plant Breeding and CRISPR Plants Market Trends 2022, Global Industry Share, Size, Global Industry Analysis, Key Market Growth Analysis, Segments, Emerging Technologies, Opportunity and Forecast 2027

George Church on 4D Omics, Natural Computing, and the BioWeatherMap

By Allison Proffitt May 4, 2022 | George Church opened the 20th Anniversary Bio-IT World Conference & Expo last night, speaking virtually to a packed room at Boston’s Hynes Convention Center while many more attendees tuned in online.  Church, the Robert Winthrop Professor, Genetics, Harvard Medical School, spoke about both…

Continue Reading George Church on 4D Omics, Natural Computing, and the BioWeatherMap

Effect of gene expression level on CRISPR sgRNA library design

Effect of gene expression level on CRISPR sgRNA library design 0 The current CRISPR sgRNA library targets the whole genome, but some genes in the cells used in the experiment are not expressed or have little expression. Is it effective to knock out these genes? If it is determined whether…

Continue Reading Effect of gene expression level on CRISPR sgRNA library design

Biogenesis, biology and characterization of circular RNAs

Biogenesis, biology and characterization of circular RNAs Kristensen LS et al. The biogenesis, biology and characterization of circular RNAs. Nat Rev Genet. (2019) Summary text Biogenesis and properties of circRNAs Biogenesis of cirRNA Characteristics of circRNAs Discover and analyze circRNAs circRNA genome-wide analysis CircRNA site-specific analysis circRNA visualization Biological functions…

Continue Reading Biogenesis, biology and characterization of circular RNAs

Multiplexed genome regulation in vivo with hyper-efficient Cas12a

. 2022 Apr;24(4):590-600. doi: 10.1038/s41556-022-00870-7. Epub 2022 Apr 12. Lucie Y Guo #  1   2 , Jing Bian #  3 , Alexander E Davis  4 , Pingting Liu  4 , Hannah R Kempton  3 , Xiaowei Zhang  3 , Augustine Chemparathy  3 , Baokun Gu  3 , Xueqiu Lin  3 , Draven A Rane  3 , Xiaoshu Xu  3 , Ryan M…

Continue Reading Multiplexed genome regulation in vivo with hyper-efficient Cas12a

Metagenomi Presents Findings on Novel Families of Ultra Small, CRISPR Enzymes at the Keystone Symposia Precision Genome Engineering Conference

EMERYVILLE, Calif., May 03, 2022–(BUSINESS WIRE)–Metagenomi, a genetic medicines company with a versatile portfolio of next-generation gene editing tools, presented findings on the identification of novel families of compact, programmable enzymes for genome editing at the Keystone Symposia Precision Genome Engineering and Emerging Cellular Therapies Conference, on April 30. The…

Continue Reading Metagenomi Presents Findings on Novel Families of Ultra Small, CRISPR Enzymes at the Keystone Symposia Precision Genome Engineering Conference

Team develops method to increase gene editing efficiency while minimizing DNA deletion sizes

Targeting E. coli DNA pol I to DSBs increased the ratio of 1-bp deletions versus >1-bp deletions and TIS versus non-TIS. (A) Counteracting DNA resection (e.g. MRE11) by pol I fused to Cas9. The expected result is suppression of the MMEJ and SSA DNA repair pathways, which require DNA resection. (B)…

Continue Reading Team develops method to increase gene editing efficiency while minimizing DNA deletion sizes

Week ending April 23: longest patent process for Springfield inventors

The longest time between a patent being filed and granted in the week ending April 23 in Springfield was 1,670 days, according to the United States Patent and Trademark Office. The patent application was filed by Board of Trustees of Southern Illinois University for a tuning CRISPR/Cas9 activity with chemically…

Continue Reading Week ending April 23: longest patent process for Springfield inventors

Enabling genome editing research | Scientist Live

As researchers using genome editing tools move from basic research to clinical settings, high-quality ancillary materials and documentation are critical. To help researchers meet stringent quality requirements, Thermo Fisher Scientific introduced the new GMP-manufactured Gibco CTS TrueCut Cas9 Protein. TrueCut Cas9 proteins are manufactured with United States Pharmacopeia standards in…

Continue Reading Enabling genome editing research | Scientist Live

Download shrna images for free

Home Download frontiers sgrna shrna structure mediated site Download intratumoral injection shrna expressing plasmid Download intratumoral injection shrna expressing plasmid Download full text lentivirus mediated shrna targeting mutyh inhibits malignant phenotype Download silencing regiv shrna causes loss stemness properties cancer stem cells mkn45 Download upar shrna inhibit angiogenesis enhanced Download…

Continue Reading Download shrna images for free

Human stem cell modelling for heart disease of long QT syndrome

Abstract Sudden cardiac death (SCD) in otherwise healthy young people is a public health priority. Long QT syndrome (LQTS) is the commonest life-threatening cardiac arrhythmia contributing to SCD under 40 years old with an estimated prevalence of 1:2,000. Cardiac rhythm is driven by coordinated cardiac action potential, which is triggered…

Continue Reading Human stem cell modelling for heart disease of long QT syndrome

Unlocked enzyme structure shows how strigolactone hormone controls plant growth

Using a permanently unlocked form of the plant enzyme MAX3, UC Davis researchers are exploring the mechanism by which the plant hormone strigoractone regulates a massive network of genes controlling plant growth and architecture. Credit: Nitzan Shabek/UC Davis As sessile organisms, plants have to continually adapt their growth and architecture…

Continue Reading Unlocked enzyme structure shows how strigolactone hormone controls plant growth

Postdoc Fellow on Cancer Genomics, Epigenetics, Metabolism, and Bioinformatics

Multiple postdoctoral positions are available in the lab of Dr. Jian Xu at Children’s Research Institute (CRI), UT Southwestern Medical Center to study the gene regulatory processes that control stem cell development and cancers. We employ genomics, epigenetics, genome editing, metabolomics, and mouse genetics to define disease-associated epigenetic and genetic programs…

Continue Reading Postdoc Fellow on Cancer Genomics, Epigenetics, Metabolism, and Bioinformatics

What’s the timeline for gene edited babies? 25 years, says CRISPR pioneer Jennifer Doudna

The GLP Needs Your Help It is easier than ever for advocacy groups to spread disinformation on pressing science issues, such as the ongoing coronavirus pandemic. No, vaccines are not harmful. Yes, the use of biotechnology, GMOs or gene editing to develop antigens for treatments including vaccines are part of…

Continue Reading What’s the timeline for gene edited babies? 25 years, says CRISPR pioneer Jennifer Doudna

Genome Editing Market Size Value US$ 16.98 billion by 2028; grow at a CAGR of 18.4%

According to our new research study on “Genome Editing Market Forecast to 2028 – COVID-19 Impact and Global Analysis – by Technology, Application, and End User,” the market is expected to grow from US$ 5.19 billion in 2021 to US$ 16.98 billion by 2028; it is estimated to grow at…

Continue Reading Genome Editing Market Size Value US$ 16.98 billion by 2028; grow at a CAGR of 18.4%

CRISPR/Cas9 deletions induce adverse on-target genomic effects

Clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) have transformed genome engineering techniques. Numerous toolsets have been created to enable easy and efficient loss-of-function perturbations of functional genomic sites. Study: CRISPR/Cas9 deletions induce adverse on-target genomic effects leading to functional DNA in human cells. Image Credit: elenabsl/Shutterstock The CRISPR/Cas9 system’s elements…

Continue Reading CRISPR/Cas9 deletions induce adverse on-target genomic effects

Genome Editing of Human Primary T Cells With Lipid Nanoparticles

By Reka Geczy, PhD, Aruna Balgi, Stella Park, Rita Zhao, Ethan Watt, Maggie Wong, Cooper Webb, Nikita Jain, PhD, Angela Zhang, PhD, Anitha Thomas, PhD, Samuel Clarke, PhD The expression of the chimeric antigen receptor (CAR) on T cells turns a patient’s cells into cell-based cancer therapies and has revolutionized…

Continue Reading Genome Editing of Human Primary T Cells With Lipid Nanoparticles

Research team follows nearly 8,000 paths towards better cell factories

Saccharomyces cerevisiae, SEM image. Credit: Mogana Das Murtey and Patchamuthu Ramasamy/CC BY-SA 3.0 Microorganisms that efficiently convert plant biomass into renewable biofuels and biochemicals play a major role in the sustainable society of the future. However, the efficiency of these microbial cell factories is inhibited by several compounds that are…

Continue Reading Research team follows nearly 8,000 paths towards better cell factories

Rat Model Market revenue worth USD 2.3 Billion by 2028,

Selbyville, Delaware, April 26, 2022 (GLOBE NEWSWIRE) — The rat model market value is projected to reach USD 2.3 billion by 2028, according to a new research report by Global Market Insights Inc. Growing consumption of personalized medicine and increasing number of clinical trials is expected to augment the market…

Continue Reading Rat Model Market revenue worth USD 2.3 Billion by 2028,

CRISPR Technology Market Size, Share, Demand, Assessment & Opportunity Forecast 2021-2030

Global CRISPR Technology Market Size study, By Product and Service (Hand-Emplaced, Air-Delivered), By Applications (Seismic, Acoustic, Magnetic, Infrared), By End User (Security {Military, Public Security} Critical Infrastructure {Utilities, Industrial & Commercial Facilities}), and Regional Forecasts 2022-2028 Global CRISPR Technology Market is valued approximately USD 1097.66 million in 2021 and is anticipated…

Continue Reading CRISPR Technology Market Size, Share, Demand, Assessment & Opportunity Forecast 2021-2030

PhD in functional genomics, antifungal discovery, synthetic biology (1.0 FTE)

Category Research / Academic Location Groningen The group of Dr. Sonja Billerbeck has an open position for a 4-year PhD project. In the Billerbeck lab, we use a combination of synthetic biology, genome engineering, protein engineering, and environmental microbiology to access, understand and engineer the functional diversity of nature’s yeast-based…

Continue Reading PhD in functional genomics, antifungal discovery, synthetic biology (1.0 FTE)

CRISPR Gene-Editing Market May See a Big Move by 2029

Global Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Gene-Editing Market Synopsis: The finest Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Gene-Editing Market research report contains the drivers and restraints for the market that are obtained with the help of SWOT analysis, and also shows all the recent developments, product launches,…

Continue Reading CRISPR Gene-Editing Market May See a Big Move by 2029

Full Text Journal Article: Exploiting the orthogonal CRISPR-Cas12a/Cas13a trans-cleavage for dual-gene virus detection using a handheld device.

PDF.MPro – Full Text Journal Article: Exploiting the orthogonal CRISPR-Cas12a/Cas13a trans-cleavage for dual-gene virus detection using a handheld device. Home / Search / Abstract 34653714 (Page 1) Advertisement Find full text journal articles PMID: 34653714 (view PubMed database entry)DOI: 10.1016/j.bios.2021.113701 (read at publisher’s website ) Tian Tian, Zhiqiang Qiu, Yongzhong…

Continue Reading Full Text Journal Article: Exploiting the orthogonal CRISPR-Cas12a/Cas13a trans-cleavage for dual-gene virus detection using a handheld device.

Science Papers on Cancer Mutational Signatures, System to Deliver CRISRP-Cas9 Machinery Across Blood-Brain Barrier

By sequencing tumors from thousands of cancer patients and comparing them to similar datasets generated by other research groups, a team led by scientists from the University of Cambridge has identified new mutational signatures of cancer, while also uncovering details about organ-specific common and rare signatures. In the study, which…

Continue Reading Science Papers on Cancer Mutational Signatures, System to Deliver CRISRP-Cas9 Machinery Across Blood-Brain Barrier

Global KRAS Inhibitors Market & Clinical Trials Forecast Report to 2028 – ResearchAndMarkets.com

DUBLIN–(BUSINESS WIRE)–The “Global KRAS Inhibitors Market & Clinical Trials Forecast 2028” report has been added to ResearchAndMarkets.com‘s offering. The advancement in clinical research has led to the identification of several oncogenes that are generally mutated in cancers. Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) is the most frequently mutated…

Continue Reading Global KRAS Inhibitors Market & Clinical Trials Forecast Report to 2028 – ResearchAndMarkets.com

03 Postdoctoral Fellowship at European Molecular Biology Laboratory (EMBL), Germany

European Molecular Biology Laboratory (EMBL), Germany invites online Application for various Postdoctoral Fellowship in their different Departments. We are providing a list of Postdoc Fellowship positions available at European Molecular Biology Laboratory (EMBL), Germany. Eligible candidate may Apply as soon as possible.   (01) Postdoctoral Fellowship Position Postdoc Fellowship Position…

Continue Reading 03 Postdoctoral Fellowship at European Molecular Biology Laboratory (EMBL), Germany

Oligonucleotide Pool Library Market 2021-2026 Detailed Analysis and Growth Strategies, Regional and Recent Scenario Analysis

The new study on the Global Oligonucleotide Pool Library Market 2020-2026 analyzes the crucial capabilities, key infrastructures, major organizations, evaluates measures to attain potential success in the Oligonucleotide Pool Library industry. Because of the in-depth overview of the world Oligonucleotide Pool Library market, the recent document allows investors, traders and…

Continue Reading Oligonucleotide Pool Library Market 2021-2026 Detailed Analysis and Growth Strategies, Regional and Recent Scenario Analysis

Researchers boost rice yield by overcoming tr

image: The mechanism of boosting rice yield by overcoming a trait trade-off beteen tiller number and panicle size view more  Credit: IGDB Crop breeding is largely constrained by tradeoffs among different agronomic traits. Since many of these tradeoffs are caused by gene pleiotropy, reducing gene pleiotropy may make it easier to…

Continue Reading Researchers boost rice yield by overcoming tr

TF differentiation of hiPSCs at Wellcome Sanger Institute

Salary per annum: c.£33,900 Contract Length: 2 years Here at the world-famous and internationally respected Wellcome Sanger Institute, we have an exciting opportunity for a postdoctoral researcher to perform high throughput screening for transcription factor-driven differentiation in human pluripotent stem cells (hiPSCs) in order to create better in vitro differentiated cells that more faithfully…

Continue Reading TF differentiation of hiPSCs at Wellcome Sanger Institute

Crispr/Cas13a Induced Exponential Amplification for Highly Sensitive and Specific Detection of Circular Rna by Pengfei Song, Pengbo Zhang, Ke Qin, Fengxia Su, Kejian Gao, Xin Liu, Zhengping Li :: SSRN

19 Pages Posted: 23 Mar 2022 See all articles by Pengfei Song University of Science and Technology Beijing University of Science and Technology Beijing University of Science and Technology Beijing University of Science and Technology Beijing University of Science and Technology…

Continue Reading Crispr/Cas13a Induced Exponential Amplification for Highly Sensitive and Specific Detection of Circular Rna by Pengfei Song, Pengbo Zhang, Ke Qin, Fengxia Su, Kejian Gao, Xin Liu, Zhengping Li :: SSRN

CRISPR-VAE: A Method for Explaining CRISPR/Cas12a Predictions, and an Efficiency-aware gRNA Sequence Generator

Abstract Deep learning has shown great promise in the prediction of the gRNA efficiency, which helps optimize the engineered gRNAs, and thus has greatly improved the usage of CRISPR-Cas systems in genome editing. However, the black box prediction of deep learning methods does not provide adequate explanation to the factors…

Continue Reading CRISPR-VAE: A Method for Explaining CRISPR/Cas12a Predictions, and an Efficiency-aware gRNA Sequence Generator

Oligonucleotide Pool Library Market Report 2022 By Segmentations, Key Company Profiles, Types, Applications & Regional Forecasts to 2030

The Oligonucleotide Pool Library market report offers a deep dive assessment of the major trends influencing the expansion trajectory of Oligonucleotide Pool Library market. This research document is strategically curated with actionable insights on the pivotal aspects of the industry sphere to empower you with in depth market intelligence. It…

Continue Reading Oligonucleotide Pool Library Market Report 2022 By Segmentations, Key Company Profiles, Types, Applications & Regional Forecasts to 2030

Know Market Dynamics, Opportunities and Risks 2027

The Oligonucleotide Pool Library Market Size and Forecast to 2027, this report provides an analysis of the impact of the COVID19 epidemic on the key points influencing the growth of the market. In addition, SaaS Online Video Platforms market segments (by major players, types, applications, and major regions) outlook, company…

Continue Reading Know Market Dynamics, Opportunities and Risks 2027

Label-Free Resonance Rayleigh Scattering Amplification for Lipopolysaccharide Detection and Logical Circuit by CRISPR/Cas12a-Driven Guanine Nanowire Assisted Non-Cross-Linking Hybridization Chain Reaction,Analytical Chemistry

Although the CRISPR/Cas system has pioneered a new generation of analytical techniques, there remain many challenges in developing a label-free, accurate, and reliable CRISPR/Cas-based assay for reporting the levels of low abundance biomolecules in complex biological samples. Here, we reported a novel CRISPR-derived resonance Rayleigh scattering (RRS) amplification strategy and…

Continue Reading Label-Free Resonance Rayleigh Scattering Amplification for Lipopolysaccharide Detection and Logical Circuit by CRISPR/Cas12a-Driven Guanine Nanowire Assisted Non-Cross-Linking Hybridization Chain Reaction,Analytical Chemistry

CRISPRi for specific inhibition of miRNA clusters and miRNAs with high sequence homology

Bartel, D. P. MicroRNAs: Target recognition and regulatory functions. Cell 136, 215–233 (2009). CAS  Article  Google Scholar  Bassett, A. R. et al. Understanding functional miRNA–target interactions in vivo by site-specific genome engineering. Nat. Commun. 5, 4640 (2014). ADS  CAS  Article  Google Scholar  Moore, M. J. et al. miRNA–target chimeras reveal…

Continue Reading CRISPRi for specific inhibition of miRNA clusters and miRNAs with high sequence homology

Nature Papers on CRISPR-Cas9 Gene Editing, HSC Development

New details about the mechanism by which DNA is unwound so that its target sequence can be read during CRISPR-Cas9 gene editing are reported in this week’s Nature Structural & Molecular Biology. The study provides insights that may help in the development of improved genome editors. During CRISPR-Cas9 editing, Cas9…

Continue Reading Nature Papers on CRISPR-Cas9 Gene Editing, HSC Development

CASCADE: Naked eye-detection of SARS-CoV-2 using Cas13a and gold nanoparticles

This article was originally published here Anal Chim Acta. 2022 May 1;1205:339749. doi: 10.1016/j.aca.2022.339749. Epub 2022 Mar 22. ABSTRACT The COVID-19 pandemic has brought to light the need for fast and sensitive detection methods to prevent the spread of pathogens. The scientific community is making a great effort to design…

Continue Reading CASCADE: Naked eye-detection of SARS-CoV-2 using Cas13a and gold nanoparticles

RCSB PDB – 7V94: Cryo-EM structure of the Cas12c2-sgRNA-target DNA ternary complex

RNA-guided CRISPR-Cas nucleases are widely used as versatile genome-engineering tools. Recent studies identified functionally divergent type V Cas12 family enzymes. Among them, Cas12c2 binds a CRISPR RNA (crRNA) and a trans-activating crRNA (tracrRNA) and recognizes double-stranded DNA targets with a short TN PAM … RNA-guided CRISPR-Cas nucleases are widely used…

Continue Reading RCSB PDB – 7V94: Cryo-EM structure of the Cas12c2-sgRNA-target DNA ternary complex

Multiplexed genome regulation in vivo with hyper-efficient Cas12a,Nature Cell Biology

Multiplexed modulation of endogenous genes is crucial for sophisticated gene therapy and cell engineering. CRISPR–Cas12a systems enable versatile multiple-genomic-loci targeting by processing numerous CRISPR RNAs (crRNAs) from a single transcript; however, their low efficiency has hindered in vivo applications. Through structure-guided protein engineering, we developed a hyper-efficient Lachnospiraceae bacterium Cas12a…

Continue Reading Multiplexed genome regulation in vivo with hyper-efficient Cas12a,Nature Cell Biology

A general RPA-CRISPR/Cas12a sensing platform for Brucella spp. detection in blood and milk samples

Available online 9 April 2022, 131864 doi.org/10.1016/j.snb.2022.131864Get rights and content Highlights • We firstly introduce a general RPA-CRISPR/Cas12a sensing platform into fluorescent and electrochemical dual-signal output. • We firstly established an isothermal, rapid, specific and ultrasensitive RPA-CRISPR/Cas12a sensing platform for Brucella spp. detection. • This platform enabled rapid and accurate…

Continue Reading A general RPA-CRISPR/Cas12a sensing platform for Brucella spp. detection in blood and milk samples

Scientists Are Using CRISPR Gene Editing to Try to Create Hypoallergenic Cats

There’s been a lot of buzz in the last few years about the perils of using CRISPR gene editing on human germline cells—the reproductive cells that determine the DNA of every other cell in the body. The idea of CRISPR babies (hypothetical as far as we know, except in the…

Continue Reading Scientists Are Using CRISPR Gene Editing to Try to Create Hypoallergenic Cats

Gene Editing Methods for Gene Therapy

Scientists at Creative Biolabs provide TALENs and CRISPR/Cas gene editing services to clients all over the world enabling investigators to disrupt, add, and correct any gene in a diverse range of cell types and organisms…. More Scientists at Creative Biolabs provide TALENs and CRISPR/Cas gene editing services to clients all…

Continue Reading Gene Editing Methods for Gene Therapy

sgRNA design tool for CRISPR/Cas9 experiments targeting transcription factor motifs

%PDF-1.7 % 1 0 obj >/Metadata 4 0 R/Pages 2 0 R/StructTreeRoot 3 0 R/Type/Catalog/ViewerPreferences 5 0 R>> endobj 4 0 obj >stream Microsoft® Word 2019 application/pdf Marta Kazimierska TransCRISPR – sgRNA design tool for CRISPR/Cas9 experiments targeting transcription factor motifs Microsoft® Word 2019 2022-03-31T12:15:15+02:00 2022-04-07T11:33:55-07:00 2022-04-07T11:33:55-07:00 uuid:D44C43A8-2A40-4F2D-8CFE-62419359C244 uuid:25f34f1a-1dd2-11b2-0a00-380000000000 endstream endobj…

Continue Reading sgRNA design tool for CRISPR/Cas9 experiments targeting transcription factor motifs

Parkinson’s disease motor symptoms rescue by CRISPRa-reprogramming astrocytes into GABAergic neurons

doi: 10.15252/emmm.202114797. Online ahead of print. Jessica Giehrl-Schwab #  1   2 , Florian Giesert #  1   2 , Benedict Rauser  1   2 , Chu Lan Lao  3   4 , Sina Hembach  1   2 , Sandrine Lefort  5 , Ignacio L Ibarra  6 , Christina Koupourtidou  3   7 , Malte Daniel Luecken  6 , Dong-Jiunn Jeffery…

Continue Reading Parkinson’s disease motor symptoms rescue by CRISPRa-reprogramming astrocytes into GABAergic neurons

Pacific Biosciences, Corteva Agriscience Collaborate on High-Throughput Sequencing

NEW YORK – Pacific Biosciences said on Monday that it is collaborating with Corteva Agriscience to develop custom workflows for plant, pest, and microbial sequencing. Under the terms of the project, the partners will establish high-throughput workflows for DNA extraction and library preparation to enable sequencing of thousands of samples….

Continue Reading Pacific Biosciences, Corteva Agriscience Collaborate on High-Throughput Sequencing

Statistical Geneticist / Bioinformatics Scientist at Illumina

Job details Job type full-time Full job description OverviewSince 2001, the cost of dna sequencing has dropped more than 100,000-fold, from $100,000,000 usd per human genome to less than $600 usd todayThis is resulting in the explosive growth of genomics data, which we are combining with machine learning for data-driven…

Continue Reading Statistical Geneticist / Bioinformatics Scientist at Illumina

MAGeCK – BioGrids Consortium – Supported Software

AllHigh-Throughput SequencingGenomicsProteomicsVisualizationOther MAGeCK Description (Model-based Analysis of Genome-wide CRISPR-Cas9 Knockout) a computational tool to identify important genes from the recent genome-scale CRISPR-Cas9 knockout screens (or GeCKO) technology. Installation Use the following command to install this title with the CLI client: $ biogrids-cli install mageck Copy to clipboard Primary Citation* W….

Continue Reading MAGeCK – BioGrids Consortium – Supported Software

Researchers identify sustainable source of im

image: Monkey iPSCs with edited CCR5 locus growing in culture. view more  Credit: Image Credit: Saritha D’Souza, Wisconsin National Primate Research Center, USA Genetic engineering can make immune cells resistant to infection with human or monkey (simian) immunodeficiency virus (HIV and SIV, respectively). Recently, treatment with HIV-resistant immune cells has given…

Continue Reading Researchers identify sustainable source of im

What is genetic engineering and how can it benefit healthcare?

Have you heard of purple tomatoes? Or golden rice? If you haven’t, you might be surprised to know that these have actually been created by scientists via genetic engineering. These are genetically modified foods, with purple tomatoes containing 10 times more antioxidants than a normal red tomato. On the other…

Continue Reading What is genetic engineering and how can it benefit healthcare?

CRISPR-Cas13d for CHO Cell Engineering and Antibody Production

Abstract Chinese hamster ovary (CHO) cell is the predominant cell factory to produce biopharmaceuticals such as immunoglobulin G (IgG), but in CHO cells lactate accumulation and cell death compromise IgG production while fucosylation mitigates the antibody-dependent cellular cytotoxicity (ADCC) of IgG. To tackle these problems, we harnessed a burgeoning gene…

Continue Reading CRISPR-Cas13d for CHO Cell Engineering and Antibody Production

2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development

We present detailed protocols for the generation and characterization of 2D and 3D human induced pluripotent stem cell (hIPSC)-based models of neocortical development as well as complementary methodologies enabling qualitative and quantitative analysis of primary cilium (PC) biogenesis and function. The overall goal of the procedure we detail here is…

Continue Reading 2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development

A CRISPR Kitty? Gene Editing Breathes New Life into the Hypoallergenic Cat

The ability to edit an organism’s genome using CRISPR has provided myriad applications ranging from crop development to novel therapeutics, while editing of various animal species offers hope in areas from de-extinction to xenotransplantation. But what about the domestic cat? In a new report published in The CRISPR Journal, researchers…

Continue Reading A CRISPR Kitty? Gene Editing Breathes New Life into the Hypoallergenic Cat

Gann and Dharwadker are Recipients of Awards by the Society for In Vitro Biology

Photo Submitted (Right) Dominic Dharwadker, undergraduate student, Honors Biochemistry; (Left) Peter James Gann, doctoral student, Cell and Molecular Biology. A doctoral Fulbright scholar from the Cell and Molecular Biology (CEMB) Program and an undergraduate student in Honors Biochemistry from the Department of Chemistry and Biochemistry are winners…

Continue Reading Gann and Dharwadker are Recipients of Awards by the Society for In Vitro Biology

dCas9-VP64-Blasticidin SAM CRISPRa Helper Construct 1 Plasmid DNA

This product is a lentiviral plasmid that utilizes the EF1 alpha promoter to drive expression of dCas9-VP64 and blasticidin resistance cassette linked by a 2A peptide (EF1a-dCas9-VP64-2A-Blasticidin) allowing for easy selection following successful transfection or transduction. Use Sigma′s lentiviral dCas9-VP64 plasmid for generation of lentiviral particles and efficient production of…

Continue Reading dCas9-VP64-Blasticidin SAM CRISPRa Helper Construct 1 Plasmid DNA

What are the PRLR-SLICK cattle?

Earlier this month, a type of short-haired cattle, called PRLR-SLICK, was added to a very short list of animals given a low-risk assessment by the FDA’s Center for Veterinary Medicine (CVM). What’s so special about these cattle is that they are the first intentional genomic alteration (IGA) in an animal…

Continue Reading What are the PRLR-SLICK cattle?

A transgene-free method for rapid and efficie

image: The reporter RNA enriched dual-sgRNA/CRISPR-Cas9 ribonucleoproteins (RE-DSRNP) method uses dual-sgRNA in combination with reporter RNA enriched RNPs (CRISPR-Cas9 ribonucleoproteins), which incorporates the precise targeting of dual-sgRNA systems with the increased editing rates but reduced off-target effects and cytotoxicity associated with reporter RNA-enriched RNPs, without the need for introducing transgenes, thus…

Continue Reading A transgene-free method for rapid and efficie

AAV-delivered suppressor tRNA overcomes a nonsense mutation in mice

Wang, D., Tai, P. W. L. & Gao, G. Adeno-associated virus vector as a platform for gene therapy delivery. Nat. Rev. Drug Discov. 18, 358–378 (2019). CAS  PubMed  PubMed Central  Google Scholar  Li, C. & Samulski, R. J. Engineering adeno-associated virus vectors for gene therapy. Nat. Rev. Genet. 21, 255–272…

Continue Reading AAV-delivered suppressor tRNA overcomes a nonsense mutation in mice

Self-electrochemiluminescence biosensor based on CRISPR/Cas12a and PdCuBP@luminol nanoemitter for highly sensitive detection of cytochrome c oxidase subunit III gene of acute kidney injury

Available online 22 March 2022, 114207 doi.org/10.1016/j.bios.2022.114207Get rights and content Abstract The cytochrome c oxidase subunit III (COX III) gene is a powerful biomarker for the early diagnosis of acute kidney injury. However, current methods for COX III gene detection are usually laborious and time-consuming, with limited sensitivity. Herein, we…

Continue Reading Self-electrochemiluminescence biosensor based on CRISPR/Cas12a and PdCuBP@luminol nanoemitter for highly sensitive detection of cytochrome c oxidase subunit III gene of acute kidney injury

Nanofountain Probe Electroporation system enables efficient engineering of stem cells — ScienceDaily

One of the ultimate goals of medical science is to develop personalized disease diagnostics and therapeutics. With a patient’s genetic information, doctors could tailor treatments to individuals, leading to safer and more effective care. Recent work from a team of Northwestern Engineering researchers has moved the field closer to realizing…

Continue Reading Nanofountain Probe Electroporation system enables efficient engineering of stem cells — ScienceDaily

Using CRISPR/Cas to develop biosafety materials

The diagnosis and treatment of viral infections, especially those that cause large-scale outbreaks, is urgently needed, as the lack of these measures allows epidemics to progress. A new Biosafety and Health study discusses the use of the highly efficient clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) technology to…

Continue Reading Using CRISPR/Cas to develop biosafety materials

BmPMFBP1 regulates the development of eupyrene sperm in the silkworm, Bombyx mori

This article was originally published here PLoS Genet. 2022 Mar 21;18(3):e1010131. doi: 10.1371/journal.pgen.1010131. Online ahead of print. ABSTRACT Sperm deliver the male complement of DNA to the ovum, and thus play a key role in sexual reproduction. Accordingly, spermatogenesis has outstanding significance in fields as disparate as infertility treatments and…

Continue Reading BmPMFBP1 regulates the development of eupyrene sperm in the silkworm, Bombyx mori

Team develops new process for evaluating impacts of gene edits that alter rather than ‘knock out’ DNA code

CRISPR-associated protein Cas9 (white) from Staphylococcus aureus based on Protein Database ID 5AXW. Credit: Thomas Splettstoesser (Wikipedia, CC BY-SA 4.0) A new study from scientists at ChristianaCare’s Gene Editing Institute is advancing the safety and efficacy of using CRISPR gene editing in patient treatments by demonstrating how to identify and…

Continue Reading Team develops new process for evaluating impacts of gene edits that alter rather than ‘knock out’ DNA code

Solved QUESTION 3 2 points Saved The gene shown here has

Transcribed image text: QUESTION 3 2 points Saved The gene shown here has four exons and two splice variants (A and B). Exons 3 and 4 each have their own STOP codon corresponding to spliceoforms A and B. You want to use CRISPR (without a donor template) to disrupt expression…

Continue Reading Solved QUESTION 3 2 points Saved The gene shown here has

A genome-scale screen for synthetic drivers of T cell proliferation

Abramson, J. S. et al. Transcend NHL 001: immunotherapy with the CD19-directed CAR T-cell product JCAR017 results in high complete response rates in relapsed or refractory B-cell non-Hodgkin lymphoma. Blood 128, 4192–4192 (2016). Google Scholar  Shifrut, E. et al. Genome-wide CRISPR screens in primary human T cells reveal key regulators…

Continue Reading A genome-scale screen for synthetic drivers of T cell proliferation

Sigma-Aldrich Files Opposition to CVC Substantive Preliminary Motion No. 1 to be Accorded Priority Benefit | McDonnell Boehnen Hulbert & Berghoff LLP

On February 18th, Sigma-Aldrich filed its Opposition to Junior Party’s (the University of California, Berkeley; the University of Vienna; and Emmanuelle Charpentier; collectively, “CVC”) Substantive Preliminary Motion No. 1 in Interference No. 106,132, asking the Patent Trial and Appeal Board for benefit of priority to U.S. Provisional Application No. 61/652,086,…

Continue Reading Sigma-Aldrich Files Opposition to CVC Substantive Preliminary Motion No. 1 to be Accorded Priority Benefit | McDonnell Boehnen Hulbert & Berghoff LLP

Cutting Edge Agriculture Gene Editing with Cas-CLOVER

Blog Agriculture Biotechnology Though CRISPR-Cas enabled targeted genome engineering across a vast array of organisms, the system features major disadvantages. Frequent off-target mutagenesis, licensing restrictions and non-ideal economic license terms have inhibited commercial crop-science product upscaling, and, in many cases, entirely disqualified CRISPR’s use by many commercial crop developers. Seeking…

Continue Reading Cutting Edge Agriculture Gene Editing with Cas-CLOVER

CRISPR/Cas12a-Enhanced Loop-Mediated Isothermal Amplification for the Visual Detection of Shigella flexneri

doi: 10.3389/fbioe.2022.845688. eCollection 2022. Affiliations Expand Affiliations 1 Provincial Key Laboratory for Transfusion-Transmitted Infectious Diseases, Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, China. 2 Clinical Laboratory Department, Yan’an Hospital Affiliated to Kunming Medical University, Kunming, China. 3 Department of Emergency, The Traditional…

Continue Reading CRISPR/Cas12a-Enhanced Loop-Mediated Isothermal Amplification for the Visual Detection of Shigella flexneri

Cho Cell Line Development & Engineering: Impact On Biopharmaceutical Production: Essay Example, 2571 words

1. Introduction The approval of Chinese hamster ovary (CHO) cell cultures for the manufacturing of protein therapeutic products modernized conventional medicine. CHO cells remained the workhorse for the production of monoclonal antibodies (mAbs) for the last at least 20 years for different reasons. First, CHO cells are easily adapting and…

Continue Reading Cho Cell Line Development & Engineering: Impact On Biopharmaceutical Production: Essay Example, 2571 words

Establishment and Genetic Manipulation of Murine Hepatocyte Organoids

A long-term ex vitro 3D organoid culture system was established from mouse hepatocytes. These organoids can be passaged and genetically manipulated by lentivirus infection of shRNA/ectopic construction, siRNA transfection and CRISPR-Cas9 engineering. The liver is the biggest organ in mammals. It plays very important role in metabolism and detoxification. Although…

Continue Reading Establishment and Genetic Manipulation of Murine Hepatocyte Organoids

secDrug: a pipeline to discover novel drug combinations to kill drug-resistant multiple myeloma cells using a greedy set cover algorithm and single-cell multi-omics

This article was originally published here Blood Cancer J. 2022 Mar 9;12(3):39. doi: 10.1038/s41408-022-00636-2. ABSTRACT Multiple myeloma, the second-most common hematopoietic malignancy in the United States, still remains an incurable disease with dose-limiting toxicities and resistance to primary drugs like proteasome inhibitors (PIs) and Immunomodulatory drugs (IMiDs).We have created a…

Continue Reading secDrug: a pipeline to discover novel drug combinations to kill drug-resistant multiple myeloma cells using a greedy set cover algorithm and single-cell multi-omics

Development of Cas12a-Based Cell-Free Small-Molecule Biosensors via Allosteric Regulation of CRISPR Array Expression

In nature, microbes have evolved different systems to sense external stimuli. Synthetic biology approaches (1) repurpose these systems as biosensors to specifically and sensitively detect various targets of interest. Although various highly sensitive and specific laboratory-based analytical methods (including high-performance liquid chromatography and mass spectrometry) can detect small-molecule targets, they…

Continue Reading Development of Cas12a-Based Cell-Free Small-Molecule Biosensors via Allosteric Regulation of CRISPR Array Expression

CRISPR/Cas12a-based technology: A powerful tool for biosensing in food safety

Background: In the context of the current pandemic caused by the novel coronavirus, molecular detection is not limited to the clinical laboratory, but also faces the challenge of the complex and variable real-time detection fields. A series of novel coronavirus events were detected in the process of food cold chain…

Continue Reading CRISPR/Cas12a-based technology: A powerful tool for biosensing in food safety

Intellia gets upgrade at Brookline amid ‘unchanged’ patent risk on CRISPR/Cas9 technology

Andy/iStock via Getty Images Intellia Therapeutics (NTLA -3.1%) was upgraded by Brookline Capital Management to Buy from Hold with a $91 price target. Brookline analyst Leah Cann said the patent risk is unchanged, but the valuation has become attractive. In February, the U.S. Patent and Trademark Office backed the Broad…

Continue Reading Intellia gets upgrade at Brookline amid ‘unchanged’ patent risk on CRISPR/Cas9 technology

Caribou Biosciences to Present at Upcoming Investor Conferences

Get instant alerts when news breaks on your stocks. Claim your 1-week free trial to StreetInsider Premium here. BERKELEY, Calif., March 07, 2022 (GLOBE NEWSWIRE) — Caribou Biosciences, Inc. (Nasdaq: CRBU), a leading clinical-stage CRISPR genome-editing biopharmaceutical company, announced today that Rachel Haurwitz, Ph.D., Caribou’s president and chief executive officer,…

Continue Reading Caribou Biosciences to Present at Upcoming Investor Conferences

Endothelial Cell Differentiation from iPSCs – iPSC Differentiation Service – Stem Cell Services – Service – Research

iPSC-Derived Endothelial Cells Optimized iPSC Generation & Differentiation Protocols High Purity & Well Characterized iPSC-derived endothelial cells (EC) are valuable tools that can help researchers better understand vascular diseases. At ASC, we use a fast, cost-effective differentiation procedure that allows our team to deliver high-quality cells in…

Continue Reading Endothelial Cell Differentiation from iPSCs – iPSC Differentiation Service – Stem Cell Services – Service – Research

EDIT Stock Escalated Afterhours Following Favorable Decision by USPTO

Editas Medicine, Inc. (EDIT) is a leading genome editing company engaged in translating the potential of the CRISPR/Cas12a and CRISPR/Cas9 genome editing platforms into a vigorous treatment pipeline for people with unmet medical needs. The price of EDIT stock during the regular trading on February 28, 2022, was $17.1 with…

Continue Reading EDIT Stock Escalated Afterhours Following Favorable Decision by USPTO

Bioconductor – TAPseq

DOI: 10.18129/B9.bioc.TAPseq     This package is for version 3.12 of Bioconductor; for the stable, up-to-date release version, see TAPseq. Targeted scRNA-seq primer design for TAP-seq Bioconductor version: 3.12 Design primers for targeted single-cell RNA-seq used by TAP-seq. Create sequence templates for target gene panels and design gene-specific primers using…

Continue Reading Bioconductor – TAPseq

CVC maintains strong US patent portfolio covering CRISPR/Cas9 despite PTAB ruling, reports ERS Genomics

The PTAB decision makes a clear distinction between the CVC applications involved in the interference and CVC’s large portfolio of issued US patents that were not involved in this interference, stating that; “There is no dispute in this proceeding that the CVC inventors conceived of a generic sgRNA CRISPR-Cas9 system…

Continue Reading CVC maintains strong US patent portfolio covering CRISPR/Cas9 despite PTAB ruling, reports ERS Genomics

Amide Internucleoside Linkages Are Well Tolerated in Protospacer Adjacent Motif-Distal Region of CRISPR RNAs

The development of CRISPR-Cas9 mediated gene editing technology is revolutionizing molecular biology, biotechnology, and medicine. However, as with other nucleic acid technologies, CRISPR would greatly benefit from chemical modifications that optimize delivery, activity, and specificity of gene editing. Amide modifications at certain positions of short interfering RNAs have been previously…

Continue Reading Amide Internucleoside Linkages Are Well Tolerated in Protospacer Adjacent Motif-Distal Region of CRISPR RNAs

A combinatorial CRISPR-Cas12a attack on HIV DNA: Molecular Therapy

CRISPR-Cas12a is an alternative class 2 gene-editing tool that may cause less off-target effects than the original Cas9 system. We have previously demonstrated that Cas12a attack with a single CRISPR RNA (crRNA) can neutralize all infectious HIV in an infected T cell line in cell culture. However, we demonstrated that…

Continue Reading A combinatorial CRISPR-Cas12a attack on HIV DNA: Molecular Therapy

Chinese researchers develop fragrant sorghum with gene editing

A woman works in sorghum field in Yangling Village of Qicun Township in Zaozhuang City, east China’s Shandong Province, Sept. 7, 2021. (Photo by Sun Zhongzhe/Xinhua) Chinese researchers have successfully cultivated fragrant sorghum using CRISPR/Cas9 gene-editing technology. Aroma is an important quality in food. Jasmine rice, for instance, is popular…

Continue Reading Chinese researchers develop fragrant sorghum with gene editing

Drosophila carrying epilepsy-associated variants in the vitamin B6 metabolism gene PNPO display allele- and diet-dependent phenotypes

Pyridox(am)ine 5′-phosphate oxidase (PNPO; Enzyme Commission Number 1.4.3.5) is a rate-limiting enzyme in the synthesis of vitamin B6 (VB6) (1). Mutations in PNPO can cause neonatal epileptic encephalopathy, a devastating disease that usually leads to death if untreated (2). Recently, PNPO mutations have also been reported in patients with infantile…

Continue Reading Drosophila carrying epilepsy-associated variants in the vitamin B6 metabolism gene PNPO display allele- and diet-dependent phenotypes

LAMP-CRISPR-Cas12a-lateral flow immunochromatographic strip | IDR

Introduction About 700,000 people die from “superbugs” globally every year. Antibiotic abuse is the culprit of extensive spread of superbugs. Currently, carbapenemase-producing organisms are among the most important pathogens of hospital infections. Especially the plasmid-mediated highly transmissible carbapenem-resistant Enterobacterales (CRE) has become an important public health issue of global concern….

Continue Reading LAMP-CRISPR-Cas12a-lateral flow immunochromatographic strip | IDR

Validea Motley Fool Strategy Daily Upgrade Report – 2/26/2022

The following are today’s upgrades for Validea’s Small-Cap Growth Investor model based on the published strategy of Motley Fool. This strategy looks for small cap growth stocks with solid fundamentals and strong price performance. EXLSERVICE HOLDINGS, INC. (EXLS) is a mid-cap growth stock in the Business Services industry. The rating…

Continue Reading Validea Motley Fool Strategy Daily Upgrade Report – 2/26/2022