Tag: CRISPRa

2023 gene therapy research STAR Grant winners announced

At PacBio, enabling the promise of genomics to better human health cuts right to the core of everything we do.That’s why in 2023 we created the STAR (Student Travel Awarded Researcher) Grant program. This exciting funding opportunity is intended to assist up-and-coming researchers in genomics-aligned fields with sequencing services and…

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Crispr Library Download

CRISPR guide RNA libraries have been iteratively improved to provide increasingly efficient reagents, although their large size is a barrier for many applications. We design an optimised minimal genome-wide human CRISPR-Cas9 library (MinLibCas9) by mining existing large-scale gene loss-of-function datasets, resulting in a greater than 42% reduction in size compared…

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Human CD26 (DPP4) activation kit by CRISPRa Clinisciences

Kit Components GA101256G1, CD26 gRNA vector 1 in pCas-Guide-GFP-CRISPRa, Target Sequence: CGACGTCATTTTTAGCTAAG GA101256G2, CD26 gRNA vector 2 in pCas-Guide-GFP-CRISPRa, Target Sequence: GAGCCGTGGGGGAGGGGAAA GA101256G3, CD26 gRNA vector 3 in pCas-Guide-GFP-CRISPRa, Target Sequence: AACCTCACGTGGACAGGCGA 1 CRISPRa-Enhancer vector, SKU GE100056 1 CRISPRa scramble vector, SKU GE100077 Disclaimer These products are manufactured and supplied…

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Human TIA1 activation kit by CRISPRa Clinisciences

Product Data Format 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) Symbol TIA1 Locus ID 7072 Kit Components GA104875G1, TIA1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa GA104875G2, TIA1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa GA104875G3, TIA1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa 1 CRISPRa-Enhancer vector, SKU GE100056 1…

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CRISPR Gene Editing: Cas9 and Beyond

Scientists use the CRISPR-Cas9 system to target a DNA sequence of interest near a protospacer-adjacent motif (PAM). The PAM initiates Cas9-DNA binding, a guide RNA (yellow) invades the double helix (blue) and hybridizes with the target DNA, and Cas9 (red) breaks the unwound double-stranded target DNA. iStock Meletios Verras Stay…

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LncRNA INHEG promotes glioma stem cell maintenance and tumorigenicity through regulating rRNA 2’-O-methylation

Ethics statement All mice procedures in this study were performed under an animal protocol approved by the Institutional Animal Care and Use Committee guidelines of Westlake University. The procedures and protocols for glioma patients were approved by the institutional review board of Beijing Tiantan Hospital. Informed consent was obtained from…

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Summary of CRISPR-Cas9 off-target Detection Methods

Genome editing technology stands at the forefront of current life science research. However, for its successful translation into clinical applications, the accurate detection of off-target effects is indispensable. Assessing and mitigating off-target effects correctly is a pressing concern. The issue of off-target effects in CRISPR-Cas9 gene editing technology has been…

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Transcriptional and epigenetic regulators of human CD8+ T cell function identified through orthogonal CRISPR screens

Developing an epigenetic screening platform in human T cells Staphylococcus aureus Cas9 (SaCas9) has been extensively used for genome editing in vivo as its compact size (3,159 bp) relative to the conventional Streptococcus pyogenes Cas9 (SpCas9) enables packaging into adeno-associated virus26,27,28. However, SaCas9 has not been widely used for targeted gene…

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IJMS | Free Full-Text | CRISPR/Cas9 Landscape: Current State and Future Perspectives

1. Introduction Genome editing has taken a leading position among genome modification technologies in a short time and is now widely used in gene therapy. To date, there are three main systems for genome editing: zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and CRISPR/Cas nucleases. Genome editing has…

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CRISPR screening in hematology research: from bulk to single-cell level | Journal of Hematology & Oncology

Transcriptomics CROP-seq [46] Jurkat Poly-A CRISPRko Cas9 119 – 5798 (+ 1320 cells with NT gRNA) Lentiviral DROP-seq RNA Perturb-seq [43, 44] K562 Barcode CRISPRi dCas9-KRAB UPR epistasis screen: 9 triplet combinations UPR Perturb-seq experiment: 91 Up to 3 gRNAs in a single vector UPR epistasis screen: 15006 UPR Perturb-seq experiment:…

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A new era of precision genome editing with CRISPR

GUS/LUC dual reporter system for detecting CRISPRa efficacy. (a) CRISPRa construct design for OsTRP-like and OsCCR1 genes. (b)Reporter construct design for OsTRP-like and OsCCR1 promoters. (c) CRISPRa efficiency measurement using two reporter genes, GUS and LUC,in Arabidopsis protoplasts. Bar charts represent mean ± sd (n = 3 three independent protoplast…

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Unlocking the genetic potential of poplars: A

The Populus genus, commonly known as poplars, cottonwoods, and aspens, consists of approximately 30 tree species native to the northern hemisphere. Because of their diverse usages in landscape, agriculture, bioenergy, and industry, Populus species have been the focus of many tree breeding and genetic improvement programs. Modern biotechnologies, including both genomics and genetic…

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Dual AAV Method Delivers DNA That’s Reassembled into Large Genes

University of Zurich scientists demonstrated that they could reassemble and express large genes using a new dual adeno-associated viral (AAV) vector technology that depends on mRNA trans-splicing. This technology, known as REVeRT (reconstitution via mRNA trans-splicing), enables versatile split site selection as well as the efficient reconstitution of various split…

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Specific Modulation of CRISPR Transcriptional Activators through RNA-Sensing Guide RNAs in Mammalian Cells and Zebrafish Embryos

Modular iSBH-sgRNA designs enable spatial separation of spacer and trigger-sensing sequences. A. In second-generation iSBH-sgRNAs, RNA triggers are complementary with the iSBH-sgRNA backfolds, thus sgRNA spacers influence RNA trigger sequences. In modular iSBH-sgRNAs, design constrains were eliminated as triggers are only complementary with the iSBH-sgRNA loop and first 15nt of…

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Arrayed CRISPR Screening for Revolutionizing Target Discovery

In this interview conducted at SLAS EU 2023 in Brussels, Belgium, we spoke to Ulrike Künzel, Associate Director in the Functional Genomics department at AstraZeneca, about the application of arrayed CRISPR screening for target identification and more.  Please could you introduce yourself and tell us what inspired your career in functional genomics?…

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Custom sgRNA Library Cloning

Take advantage of Cellecta’s 15+ years of experience making pooled sgRNA Lentiviral Libraries In addition to their ready-to-use, off-the-shelf Genome-Wide CRISPR libraries, Cellecta also provides custom-designed CRISPR sgRNA Pooled Libraries targeting virtually any set of genes and incorporating guide sequences and vector features for a range of screening applications. Custom…

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Optimization of Cas12a for multiplexed genome-scale transcriptional activation.

Cas12a CRISPR technology, unlike Cas9, allows for facile multiplexing of guide RNAs from a single transcript, simplifying combinatorial perturbations. While Cas12a has been implemented for multiplexed knockout genetic screens, it has yet to be optimized for CRISPR activation (CRISPRa) screens in human cells. Here, we develop a new Cas12a-based transactivation…

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Human Metabotropic Glutamate Receptor 4 (GRM4) activation

Product Data Format 3gRNAs, 1 scramble ctrl and 1 enhancer vector Symbol GRM4 Locus ID 2914 Kit Components GA101969G1, Metabotropic Glutamate Receptor 4 gRNA vector 1 in pCas-Guide-GFP-CRISPRa GA101969G2, Metabotropic Glutamate Receptor 4 gRNA vector 2 in pCas-Guide-GFP-CRISPRa GA101969G3, Metabotropic Glutamate Receptor 4 gRNA vector 3 in pCas-Guide-GFP-CRISPRa 1 CRISPRa-Enhancer…

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SLC26A3 CRISPRa sgRNA lentivirus (Target 1)(Human) – K2181276

Catalog Number: ABM-440681210296 Product Overview Article Name: SLC26A3 CRISPRa sgRNA lentivirus (Target 1)(Human) – K2181276 Biozol Catalog Number: ABM-440681210296 Supplier Catalog Number: 440681210296 Alternative Catalog Number: ABM-440681210296 Manufacturer: Applied Biological Materials Category: Molekularbiologie Species Reactivity: Human Product Properties Sequence: U6 Sequencing Forward Primer Target: Sequence available upon placing order Read…

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CRISPRa-based activation of Fgf21 and Fndc5 ameliorates obesity by promoting adipocytes browning

FIGURE 7 In vivo CRISPRa‐based myokines activation ameliorates glucose tolerance and insulin sensitivity in DIO… FIGURE 7 In vivo CRISPRa‐based myokines activation ameliorates glucose tolerance and insulin sensitivity in DIO mice. Glucose tolerance (left) was studied in all experimental groups after an intraperitoneal injection of glucose (2 g/kg body weight). Insulin…

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Expert Advice on CRISPR Design

  The following is an excerpt from an E-book that Twist developed for Genetic Engineering News: The Changing Landscape of CRISPR Screening: A guidebook to the latest CRISPR screening methodologies and technologies. You can download the full E-book here.   Even for seasoned researchers, designing an sgRNA library can be…

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Growth Medium 1S – Nordic Biosite

Additional Information This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines. Optimized for Use With: CRISPRa (SAM) HeLa Cell Line (BPS Bioscience, #78193), CRISPRa (SAM) HepG2 Cell Line (BPS Bioscience, #78194) Article No BPS-78221 Country Availability SE, FI, DK, NO, IS, EE, LV,…

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Multi-faceted CRISPR/Cas technological innovation aspects in the framework of 3P medicine

Golubnitschaja O, Baban B, Boniolo G, Wang W, Bubnov R, Kapalla M, et al. Medicine in the early twenty-first century: paradigm and anticipation – EPMA position paper 2016. EPMA J. 2016;7:23. Article  PubMed  PubMed Central  Google Scholar  Kropp M, Golubnitschaja O, Mazurakova A, Koklesova L, Sargheini N, Vo T-TKS, et…

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Global CRISPR Gene Editing Market: Trends, Growth, Size, Share and Forecasts (2022-2028)

According to a new report published by UnivDatos Markets Insights, the CRISPR gene editing market was valued at more than USD 800 million in 2020 and is expected to grow at a CAGR of around 18% from 2022-2028. The analysis has been segmented into Product Type (CRISPR Products and CRISPR…

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Revvity hiring Senior Bioinformatics Scientist in Cambridge, England, United Kingdom

About The RoleWe have an exciting opportunity for an experienced bioinformatician who is interested in working at the forefront of the gene editing (CRISPR knock-out, base editing) and gene modulation (CRISPRa, CRISPRi, RNAi) fields. We are seeking a Cambridge-based Senior Bioinformatics Scientist 1 to join our global Bioinformatics team, which…

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Targeted knock-ins with pseudovirus for the stable expression of large transgenes

Integrase-deficient lentivirus (IDLV) enables reverse transcription of an encoded payload from an RNA molecule into a DNA template, which creates a useful yet low-efficiency donor for HDR. We engineered the IDLV genome to include CRISPR ‘cut sites’ that match the intended genomic cut site (Fig. 1). Cas9 ribonucleoprotein electroporated into…

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Paired immunoglobulin-like receptor B is an entry receptor for mammalian orthoreovirus

Ethics statement All experiments in this study comply with guidelines of the U.S. Public Health Service and were approved by the Institutional Biosafety Committee at the University of Pittsburgh. All animal husbandry and experimental procedures were conducted in accordance with U.S. Public Health Service policy and approved by the Institutional…

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rules for choosing guides for multiplex CRISPRa an…

I was wondering if anybody has information on how to choose guides for optimal gene activation/repression using multiplex CRISPR/Cas9s with only two guides? I have tried different combinations e.g. two guides with average activity v.s. one poor guide plus an average guide and got mixed results. Sometimes the latter outperformed…

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Stable expression of large transgenes via the knock-in of an integrase-deficient lentivirus

CLIP enables high-efficiency knock-in into an essential locus To create CLIP, we leverage integrase-deficient lentiviruses (IDLV) to deliver an HDR donor template as a viral RNA genome to cells25,26. IDLV consists of lentiviral components but with a D64V mutation in the integrase, maintaining this enzyme’s ability to package and nuclear…

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Epigenetic reactivation of tumor suppressor genes with CRISPRa technologies as precision therapy for hepatocellular carcinoma

Background: Epigenetic silencing of tumor suppressor genes (TSGs) is a key feature of oncogenesis in hepatocellular carcinoma (HCC). Liver-targeted delivery of CRISPR-activation (CRISPRa) systems makes it possible to exploit chromatin plasticity, by reprogramming transcriptional dysregulation. Results: Using The Cancer Genome Atlas HCC data, we identify 12 putative TSGs with negative…

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New Opportunities For Stem Cell Research: Increasingly Efficient Production Of Human Pluripotent Stem Cells

A few years ago, a CRISPR activator (CRISPRa) gene-editing technique was successfully developed in a collaborative study carried out at the University of Helsinki, which made it possible to convert adult skin cells to pluripotent stem cells. What makes the CRISPRa technique exceptional is that the process can be implemented…

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PerkinElmer Informatics hiring Senior Bioinformatics Scientist in Cambridge, England, United Kingdom

Responsibilities Location Cambridge, CambridgeshireGB Job ID JR-036129 About The PerkinElmer Life Sciences And Diagnostics Company The PerkinElmer Life Sciences and Diagnostics company – which is to be renamed during the second quarter of 2023 – provides end-to-end solutions that help scientists, researchers and clinicians better diagnose disease and discover new…

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Optimization of Cas12a for multiplexed genome-scale transcriptional activation

Abstract Cas12a CRISPR technology, unlike Cas9, allows for multiplexing guide RNAs from a single transcript, simplifying combinatorial perturbations. While Cas12a has been implemented for multiplexed knockout genetic screens, it has yet to be optimized for CRISPR activation (CRISPRa) screens in human cells. Here we develop a new Cas12a-based transactivation domain…

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Optimization of Cas9 activity through the addition of cytosine extensions to single-guide RNAs

Cell culture We cultured mESCs in t2iL medium containing Dulbecco’s modified eagle medium (DMEM, Nacalai Tesque), 2 mM Glutamax (Nacalai Tesque), 1× non-essential amino acids (Nacalai Tesque), 1 mM sodium pyruvate (Nacalai Tesque), 100 U ml−1 penicillin, 100 μg ml−1 streptomycin (P/S) (Nacalai Tesque), 0.1 mM 2-mercaptoethanol (Sigma) and 15% fetal bovine serum (FBS) (Gibco), supplemented with…

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Targeted Transcriptional Activation in Human Cells and in the Mouse Brain Tissue by Capsid-modified AAV and Evolved CRISPRa – CityU Scholars

Transcriptional dysregulation has been implicated in a number of progressive neurodegenerative diseases. To correct transcriptional misregulation, emerging CRISPR tools such as CRISPR activation (CRISPRa) and CRISPR interference (CRISPRi) could be used. In addition, efficient and precise transcriptional activation in broad brain areas and multiple neuroanatomical structures can be achieved by…

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CRISPR-Made Greener Products and Processes in Industrial Biotechnology

By Eric Rhodes, CEO at ERS Genomics‌ CRISPR/Cas9 genome editing is fast becoming the essential tool for industrial microbial production and chemical synthesis by virtue of its speed, precision, and versatility. CRISPR can drastically improve the efficiency of industrial biotechnology through enzyme engineering and pathway modification and is already being…

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Genome-Wide Gene Regulation by Transcriptional CRISPRa/i Tools in Non-Model Bacteria

Authors: Cholpisit Kiattisewee* (cholpk@uw.edu), Ian D. Faulkner, Jesse G. Zalatan, and James M. Carothers Institutions: University of Washington URLs: Goals CRISPR activation (CRISPRa) and CRISPR interference (CRISPRi) are modular tools that can regulate gene expression of both heterologous and endogenous genes of microorganisms. The project’s goal is to use CRISPRa/i to…

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Multiplex, single-cell CRISPRa screening for cell type specific regulatory elements

Abstract CRISPR-based gene activation (CRISPRa) is a promising therapeutic approach for gene therapy, upregulating gene expression by targeting promoters or enhancers in a tissue/cell-type specific manner. Here, we describe an experimental framework that combines highly multiplexed perturbations with single-cell RNA sequencing (sc-RNA-seq) to identify cell-type-specific, CRISPRa-responsive cis-regulatory elements and the…

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Which statement describes the results for the

Transcribed image text: Which statement describes the results for the transgenic CRISPRa Sim 1+/− mice produced by the TARGATT knockin system? neither promoter nor enhancer activation leads to Sim1 upregulation and reduced weight in transgenic mice only promoter activation leads to Sim 1 upregulation and reduced weight in transgenic mice…

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CRISPR/Cas-mediated transcriptional modulation: The therapeutic promises of CRISPRa and CRISPRi

Review . 2023 Mar 23;S1525-0016(23)00145-4. doi: 10.1016/j.ymthe.2023.03.024. Online ahead of print. Affiliations Expand Affiliations 1 Department of Biomedicine, Aarhus University, 8000 Aarhus C., Denmark. 2 Department of Biomedicine, Aarhus University, 8000 Aarhus C., Denmark. Electronic address: bak@biomed.au.dk. Item in Clipboard Review Louise Bendixen et al. Mol Ther. 2023. Show details Display…

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Validated CRISPRa effect at the CNNM2 and CCDC92/ZNF664 loci do not nominate candidate causal CAD genes.

(A) Locus view for the CAD locus with nearby gene CNNM2. We provide the position of the sentinel CAD variant (rs11191416) and the putative functional variant identified in the pooled CRISPR screen (rs78260931). The LD proxies and sgRNAs tested are also shown. ATAC-seq and RNA-seq data in resting teloHAEC are…

Continue Reading Validated CRISPRa effect at the CNNM2 and CCDC92/ZNF664 loci do not nominate candidate causal CAD genes.

CRISPRa experiment with a silent sgRNA at the LPL locus does not influence cell proliferation nor the expression of senescence marker genes.

(A) teloHAEC that express dCas9-VP64 were infected with a lentivirus that carries a sgRNA that targets rs1441755 at the LPL locus. This sgRNA was silent in all our pooled CRISPR screens for all six endothelial phenotypes tested. In the absence or presence of antibiotic selection (Zeocin), LPL_sg08237 does not affect…

Continue Reading CRISPRa experiment with a silent sgRNA at the LPL locus does not influence cell proliferation nor the expression of senescence marker genes.

Writing the Rules on CRISPR Activation

Researchers from the Wellcome Sanger Institute and collaborators used human stem cells and neurons to investigate what features influence how well CRISPR activation works for different sets of genes. Their study “Massively parallel characterization of CRISPR activator efficacy in human induced pluripotent stem cells and neurons,” which appears in Molecular…

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CRISPR Activator Approaches Assayed in Human Stem Cells, Neurons

For a paper in Molecular Cell, a team from the Wellcome Sanger Institute and other centers in the UK and China share findings from a barcoded reporter-based analysis of CRISPR activation (CRISPRa) activity in human induced pluripotent stem cells (iPSCs) or differentiated neurons. Starting with iPSCs or neurons containing thousands…

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New research writes the rules on CRISPR activ

New research writes the rules on CRISPR activation  New research starts to write the rulebook on how to effectively use CRISPR activation technology. Researchers, from the Wellcome Sanger Institute and collaborators, used human stem cells and neurons to investigate what features influence how well CRISPR activation works for different sets…

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New research explores the rules of CRISPR activation

Credit: Molecular Cell (2023). DOI: 10.1016/j.molcel.2023.02.011 New research is starting to write the rulebook on how to effectively use CRISPR activation technology. Researchers from the Wellcome Sanger Institute and collaborators have used human stem cells and neurons to investigate the features that influence how well CRISPR activation works for different…

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Development of trans-acting RNA guided tools to control gene expression in bacteria

dc.contributor.advisor Chappell, James dc.creator Villegas Kcam, Maria Claudia dc.date.accessioned 2022-12-21T20:09:38Z dc.date.created 2022-12 dc.date.issued 2022-11-08 dc.date.submitted December 2022 dc.identifier.citation Villegas Kcam, Maria Claudia. “Development of trans-acting RNA guided tools to control gene expression in bacteria.” (2022) Diss., Rice University. hdl.handle.net/1911/114170. dc.identifier.uri hdl.handle.net/1911/114170 dc.description.abstract The ability to control gene expression is a…

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ZFIN Publication: Weuring et al., 2021

PUBLICATION Authors Weuring, W.J., Dilevska, I., Hoekman, J., van de Vondervoort, J., Koetsier, M., van ‘t Slot, R.H., Braun, K.P.J., Koeleman, B.P.C. ID ZDB-PUB-210819-5 Date 2021 Source The CRISPR journal   4: 575-582 (Journal) Generate reference Registered Authors Koeleman, B. P. C., van ‘t Slot, Ruben, Weuring, Wout Keywords none…

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CD Biosciences Releases Drosophila CRISPR/Cas9 Genome Editing to Study Gene Function and Drug Screening

CD Biosciences Drosophila Center focuses on micro-injection, genome editing, cell engineering, genetics, metabolism, neurobiology and other fields. With a mission to provide high-quality and affordable Drosophila products and services, CD BioSciences with a team of experienced experts develops solutions by precise genetic modification of Drosophila as humanized models, as well…

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Mouse Metabolic Gene CRISPRa sgRNA Library

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications. For your Materials & Methods section: Mouse Metabolic Gene CRISPRa sgRNA Library was a gift…

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Whole-genome CRISPR activation for the identification of host factors controlling cellular interactions with SARS-CoV-2

In a recent study published in the PLOS Biology, researchers found that leucine-rich repeat-containing protein 15 (LRRC15), a toll-like receptor (TLR)-related cell surface receptor, blocked severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) binding. Study: Fibroblast-expressed LRRC15 is a receptor for SARS-CoV-2 spike and controls antiviral and antifibrotic transcriptional…

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Whole-genome CRISPRa screening identified LRRC15 as a novel SARS-CoV-2 spike-binding protein.

(A) Schematic of CRISPRa screen used to identify regulators of SARS-CoV-2 spike binding. (B) Ranking of all genes in screen 1 by log2 fold change (LFC) calculated using MAGeCK and plotted using MAGeCKFlute. See also S1 Table. (C) Gene enrichment analysis of screen 1 performed using MAGeCK. Horizontal dotted line…

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Screening of lymphoma radiotherapy-resistant genes

Introduction Lymphoma, a cancer characterized by a malignant tumor of the immune system that originates in the lymph nodes or lymphoid tissue, is one of the most common cancers worldwide. According to GLOBOCAN, the incidence of non-Hodgkin lymphoma (NHL) in both males and females ranked top ten among all cancers…

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CRaTER enrichment for on-target gene-editing enables generation of variant libraries in hiPSCs

Abstract Standard transgenic cell line generation requires screening 100-1000s of colonies to isolate correctly edited cells. We describe CRISPRa On-Target Editing Retrieval (CRaTER) which enriches for cells with on-target knock-in of a cDNA-fluorescent reporter transgene by transient activation of the targeted locus followed by flow sorting to recover edited cells….

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Addgene: pLKO5-SgHottip-GFP-CRISPRa

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications. For your Materials & Methods section: pLKO5-SgHottip-GFP-CRISPRa was a gift from Suming Huang (Addgene plasmid # 134990…

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The crisprVerse: a comprehensive Bioconductor ecosystem for the design of CRISPR guide RNAs across nucleases and technologies

Abstract The success of CRISPR-mediated gene perturbation studies is highly dependent on the quality of gRNAs, and several tools have been developed to enable optimal gRNA design. However, these tools are not all adaptable to the latest CRISPR modalities or nucleases, nor do they offer comprehensive annotation methods for advanced…

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Identification of orphan ligand-receptor relationships using a cell-based CRISPRa enrichment screening platform

Reviewer #1 (Public Review): In this manuscript, Siepe et al. developed a high-throughput screen designed to identify novel protein-protein interactions in the extracellular human proteome. Their CRISPRa-based method induced the expression of transmembrane receptors such that they could be screened for binding to proteins of interest. Major strengths of this…

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CRISPRa screen identifies a role for c-KIT signaling in tamoxifen resistance, potentially through upregulation of ABC transporters

Abstract Resistance to endocrine therapy is a common problem in patients with estrogen receptor alpha (ERα) positive breast cancer. In this study, we took a non-biased genome-wide approach to identify novel mechanisms of endocrine resistance using a clustered regularly interspaced short palindromic repeats (CRISPR) activating (CRISPRa) screen. Results from the…

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Parkinson’s disease motor symptoms rescue by CRISPRa-reprogramming astrocytes into GABAergic neurons

doi: 10.15252/emmm.202114797. Online ahead of print. Jessica Giehrl-Schwab #  1   2 , Florian Giesert #  1   2 , Benedict Rauser  1   2 , Chu Lan Lao  3   4 , Sina Hembach  1   2 , Sandrine Lefort  5 , Ignacio L Ibarra  6 , Christina Koupourtidou  3   7 , Malte Daniel Luecken  6 , Dong-Jiunn Jeffery…

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dCas9-VP64-Blasticidin SAM CRISPRa Helper Construct 1 Plasmid DNA

This product is a lentiviral plasmid that utilizes the EF1 alpha promoter to drive expression of dCas9-VP64 and blasticidin resistance cassette linked by a 2A peptide (EF1a-dCas9-VP64-2A-Blasticidin) allowing for easy selection following successful transfection or transduction. Use Sigma′s lentiviral dCas9-VP64 plasmid for generation of lentiviral particles and efficient production of…

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Novel CRISPR platform to decode the immune system

Date: 11th February 2022 The immune system is a critical biological network of processes that protects an organism from disease, and depends on the ability to distinguish self from non-self, a role driven by antigens.  In humans, T cells respond to antigen stimulation together with the production of cytokines however,…

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Novel CRISPR Tool Activates Instead of Editing Human Immune Cell Genes

Scientists at Gladstone Institutes and UC San Francisco (UCSF) say they have co-opted the CRISPR-Cas9 system to forcibly activate genes—rather than edit them—in human immune cells. The method, known as CRISPRa, lets them discover genes that play a role in immune cell biology more thoroughly and rapidly than previously possible,…

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Researchers use CRISPR activation method to reveal “Rosetta Stone” of immune cell function

SAN FRANCISCO, CA—February 4, 2022—CRISPR genome editing has served as a powerful tool for deleting or altering DNA sequences and studying the resulting effect. Now, researchers at Gladstone Institutes and UC San Francisco (UCSF) have co-opted the CRISPR-Cas9 system to forcibly activate genes—rather than edit them—in human immune cells. The…

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Increasingly efficient production of human pluripotent stem cells

Reliable production of high-quality iPS cells enables their increasingly efficient utilization in biomedical applications. Credit: Ras Trokovic, University of Helsinki Researchers at the University of Helsinki have developed a new, faster and more reliable technique for reverting human cells to the stem cell state. Pluripotent stem cells are a key…

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New biosensors shine a light on CRISPR gene editing — ScienceDaily

Detecting the activity of CRISPR gene editing tools in organisms with the naked eye and an ultraviolet flashlight is now possible using technology developed at the Department of Energy’s Oak Ridge National Laboratory. Scientists demonstrated these real-time detection tools in plants and anticipate their use in animals, bacteria and fungi…

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New biosensor sheds light on CRISPR gene editing

ORNL’s biosensor system reveals the CRISPR activity of poplar plants that glow bright green under UV light compared to normal plants that appear red.Credits: Guoliang Yuan / ORNL, US Department of Energy Using technology developed at the Oak Ridge National Laboratory of the Department of Energy, it is now possible…

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New biosensors shine a light on CRISPR gene editing

ORNL’s biosensor system reveals CRISPR activity in poplar plants, which glow bright green under ultraviolet light, compared to normal plants, which appear red. Credit: Guoliang Yuan/ORNL, U.S. Dept. of Energy Detecting the activity of CRISPR gene editing tools in organisms with the naked eye and an ultraviolet flashlight is now…

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Bioinformatics Scientist 2 (Internal Only)

Job Summary We have an exciting opportunity in our US office for an experienced bioinformatician who is interested in working at the forefront of the gene editing (CRISPR knock-out, HDR, base editing) and gene modulation (CRISPRa, CRISPRi, RNAi) fields. As a Bioinformatics Scientist 2, you will have the opportunity to…

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New Techniques and Complex Models

CRISPR systems that rely on inactivated Cas enzymes—that is, dead Cas (dCas) enzymes—never looked more alive. They harness the targeting power associated with CRISPR—but not the double-strand cuts. As such, they give researchers new ways to interrogate and manipulate gene function. Possibilities include CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa)…

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