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Tag: CyTOF
Immune-privileged tissues formed from immunologically cloaked mouse embryonic stem cells survive long term in allogeneic hosts
Mice C57BL/6N (strain 005304), C3H/HeJ (strain 000659), FVB/NJ (strain 001800), BALB/cJ (strain 000651) and NSG mice (stock 005557) were purchased from the Jackson Laboratory. CD-1 (stock 022) mice were purchased from Charles River. Mice (6–20-week-old) of each strain/background were used for teratoma assays. Mice were housed in a pathogen-free facility…
Insights gained from single-cell analysis of chimeric antigen receptor T-cell immunotherapy in cancer | Military Medical Research
Zhu J, Ke Y, Liu Q, Yang J, Liu F, Xu R, et al. Engineered Lactococcus lactis secreting Flt3L and OX40 ligand for in situ vaccination-based cancer immunotherapy. Nat Commun. 2022;13(1):7466. Article CAS PubMed PubMed Central Google Scholar Evans ER, Bugga P, Asthana V, Drezek R. Metallic nanoparticles for cancer…
Fasting-Mimicking Diet Drives Antitumor Immunity against Colorectal Cancer by Reducing IgA-Producing Cells | Cancer Research
Fasting-mimicking diet (FMD) is emerging as an effective dietary intervention with the potential to prolong life span in healthy people and boost antitumor immunity in patients with cancer. FMD refers to a medically designed fasting-like state that allows periodic consumption of a very-low-calorie and low-protein diet (1, 2). Compared with…
Job Opportunity: Bioinformatics Scientist, Genentech, South San Francisco, CA | BRET Career Development ASPIRE Program
Posted by hagansa2 on Wednesday, September 13, 2023 in Job Opportunities . Genentech seeks a talented and highly motivated Bioinformatics Scientist to pursue reverse translational research projects in collaboration with our Molecular Oncology and Oncology Biomarker Development Departments. Recently, exciting developments at Genentech and elsewhere have uncovered new avenues for…
FACS (Fluorescence-activated cell sorting NOT cyTOF),Automating the removal of dead and duplicated cells
FACS (Fluorescence-activated cell sorting NOT cyTOF),Automating the removal of dead and duplicated cells 0 I have a large FACS project involving hundreds of files. So, it is not practical or suitable to manually gate these in Flowjo etc and to remove dead and duplicated cells. I have found many different…
Comprehensive Characterization of the Multiple Myeloma Immune Microenvironment Using Integrated scRNA-seq, CyTOF, and CITE-seq Analysis
Comprehensive Characterization of the Multiple Myeloma Immune Microenvironment Using Integrated scRNA-seq, CyTOF, and CITE-seq Analysis As part of the Multiple Myeloma Research Foundation (MMRF) immune atlas pilot project, we compared immune cells of multiple myeloma bone marrow samples from 18 patients assessed by single-cell RNA sequencing (scRNA-seq), mass cytometry (CyTOF),…
Multiparametric senescent cell phenotyping reveals targets of senolytic therapy in the aged murine skeleton
Development and validation of a senescence CyTOF antibody panel We constructed and validated a comprehensive CyTOF antibody panel to include markers for both cell identity and senescent phenotype (Table 1). A defining characteristic of senescent cells is expression of cell cycle inhibitors, in particular p16 or p2129, so we carefully validated…
Bioinformatics Scientist – Guidehouse | Bethesda, MD
Job Family: Data Science & Analysis Travel Required: None Clearance Required: Ability to Obtain Public Trust What You Will Do: Perform computational analysis, integration, and modeling of diverse data sets, to include microarray, bulk and single-cell RNAseq, ATAC-seq, flow and CyTOF phenotyping, serum proteome, SNP genotyping, and microbiome profiling Perform…
Bioconductor – CATALYST
DOI: 10.18129/B9.bioc.CATALYST This package is for version 3.12 of Bioconductor; for the stable, up-to-date release version, see CATALYST. Cytometry dATa anALYSis Tools Bioconductor version: 3.12 Mass cytometry (CyTOF) uses heavy metal isotopes rather than fluorescent tags as reporters to label antibodies, thereby substantially decreasing spectral overlap and allowing…
Bioinformatics Scientist – Guidehouse – BETHESDA, MD
Job Family:Data Science & Analysis Travel Required:None Clearance Required:Ability to Obtain Public Trust What You Will Do: Perform computational analysis, integration, and modeling of diverse data sets, to include microarray, bulk and single-cell RNAseq, ATAC-seq, flow and CyTOF phenotyping, serum proteome, SNP genotyping, and microbiome profiling Perform “Bottom up” dynamical…
Bioinformatics Scientist – Weems Design Studio Inc
We are seeking a Bioinformatics Scientist to join the team at the National Institutes of Heath’s Center for Immuno-Oncology (CIO). This is a Federal Government Contractor position and the candidate must be able to pass a background check. Responsibilities: Perform computational analysis, integration, and modeling of diverse data sets, to…
Effects of zoledronic acid on senescence and
image: Figure 1. Zoledronic acid has senolytic effects in human lung fibroblast IMR90 cells. view more Credit: 2023 Samakkarnthai et al. “In the present study, we used multiple complementary approaches to evaluate the possible effects of zoledronic acid on cellular senescence.” BUFFALO, NY- May 15, 2023 – A new research paper…
Guidehouse Bioinformatics Scientist in Bethesda, MD | 840298899
Job Family: Data Science & Analysis Travel Required: None Clearance Required: Ability to Obtain Public Trust What You Will Do: Perform computational analysis, integration, and modeling of diverse data sets, to include microarray, bulk and single-cell RNAseq, ATAC-seq, flow and CyTOF phenotyping, serum proteome, SNP genotyping, and microbiome profiling Perform…
Guidehouse Bioinformatics Scientist in Sandy Spring, MD | 840299164
Job Family: Data Science & Analysis Travel Required: None Clearance Required: Ability to Obtain Public Trust What You Will Do: Perform computational analysis, integration, and modeling of diverse data sets, to include microarray, bulk and single-cell RNAseq, ATAC-seq, flow and CyTOF phenotyping, serum proteome, SNP genotyping, and microbiome profiling Perform…
Bioinformatics Research Officer / Assistant job with UNIVERSITY OF SYDNEY
Full time, 12 month fixed term contract with the possibility of extension contingent on funding A fantastic opportunity to perform data analysis to support the School of Mathematics & Statistics, located on Camperdown Campus Base Salary HEO5/6, $79,717 – $98,796 p.a. + 17% superannuation About the…
Applications of single-cell RNA sequencing in drug discovery and development
DiMasi, J. A., Grabowski, H. G. & Hansen, R. W. Innovation in the pharmaceutical industry: new estimates of R&D costs. J. Health Econ. 47, 20–33 (2016). Article PubMed Google Scholar Wouters, O. J., McKee, M. & Luyten, J. Estimated research and development investment needed to bring a new medicine to…
Stem cell biology | iPSC-based research in Zurich
PD Dr. Paolo Cinelli Head Center for Surgical Research and Research Laboratory Department of Trauma University Hospital Zurich and University of Zurich paolo.cinelli@usz.ch Research Focus: The main research focus of our research group is the establishment, characterization and analysis of pluripotent stem cells (ESCs, iPSCs) and in particular the study and…
Post-doctoral Research Associate (Blood Cancer UK) job with KINGS COLLEGE LONDON
Job description This is a research support post in Mishto lab within the Centre for Inflammation Biology and Cancer Immunology (CIBCI) at the Guy’s campus of King’s College London. This post is also shared with Kordasti lab at the Comprehensive Cancer Centre at the Guy’s campus of King’s College London…
CyTOF FlowCore Catalyst Question
CyTOF FlowCore Catalyst Question 0 Hi. Thank you in advance. I analyze my flow, actually CyTOF, files (fcs) using R vignettes from Malgorzata Nowicka, eta al. CyTOF workflow: differential discovery in high-throughput high-dimensional cytometry datasets (F1000 article f1000research.com/articles/6-748/v3). Everything works great. I have multiple samples from two groups (condition) and…
Research Associate – Cancer Immunology job with KINGS COLLEGE LONDON
Job description As a research associate, the post holder will be required to provide high level intellectual and technical support to the experimental work being undertaken on project(s) under the supervision of Dr Sheeba Irshad. Dr Sheeba Irshad is a medical oncologist with an interest in investigating the antigenic drivers of…
Scientist, Associate Bioinformatics – Emory University
Discover Your Career at Emory University: Emory University is a leading research university that fosters excellence and attracts world-class talent to innovate today and prepare leaders for the future. We welcome candidates who can contribute to the diversity and excellence of our academic community. Description:The Department of Hematology and Oncology…
Scientific Manager – Immunology (Nanostring, Exome, RNA, CHIP) job with University of Texas MD Anderson Cancer Center
The Immunology department at MD Anderson Cancer Center seeks to determine the fundamental cellular and molecular mechanisms of a wide range of processes by which the body recognizes and eliminates pathogens and use these findings to unleash the patient’s own immune system against previously treatment-resistant cancers. Treating the immune system…
The Phenotype and Function of CD26+ And CD161+ T Lymphocytes in Splenic Marginal Zone Lymphomas
Regulatory T-cells (Tregs) play a crucial role in maintaining immunological balance and regulating the body’s response to tumors in lymphoma patients. However, research on the role of Tregs in the biology and prognosis of splenic marginal zone lymphoma (SMZL) is lacking. CITE-seq (cellular indexing of transcriptomes and epitopes by sequencing),…
secDrug: a pipeline to discover novel drug combinations to kill drug-resistant multiple myeloma cells using a greedy set cover algorithm and single-cell multi-omics
This article was originally published here Blood Cancer J. 2022 Mar 9;12(3):39. doi: 10.1038/s41408-022-00636-2. ABSTRACT Multiple myeloma, the second-most common hematopoietic malignancy in the United States, still remains an incurable disease with dose-limiting toxicities and resistance to primary drugs like proteasome inhibitors (PIs) and Immunomodulatory drugs (IMiDs).We have created a…
NIH-SOAR-NCI-Bioinformatics Scientist in Rockville, MD
Axle Informatics is a bioscience and information technology company that offers advancements in translational research, biomedical informatics, and data science applications to research centers and healthcare organizations around the globe. With experts in biomedical science, software engineering, and program management, we focus on developing and applying research tools and techniques…
AbbVie hiring Senior Scientist I/II, Machine Learning Bioinformatics – (REMOTE Optional) in Cambridge, Massachusetts, United States
About AbbVieAbbVie’s mission is to discover and deliver innovative medicines that solve serious health issues today and address the medical challenges of tomorrow. We strive to have a remarkable impact on people’s lives across several key therapeutic areas: immunology, oncology, neuroscience, eye care, virology, women’s health and gastroenterology, in addition…