Tag: ddATP

Solved You are peforming a dideoxy chain termination

Transcribed image text: You are peforming a dideoxy chain termination sequencing reaction, and include the following components: 1. Template DNA inserted into a sequencing vector 2.Sequencing primers that anneal to the vector immediately upstream of the insertion site 3.ddATP, ddTTP.ddCTP, ddGTP fluorescently labeled with unique colors 4. DNA polymerase in…

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Normal With Genetic Disorder +ddTTP tetranucleotide

Transcribed image text: Normal With Genetic Disorder +ddTTP tetranucleotide tetranucleotide pentanucleotide pentanucleotide heptanucleotide heptanucleotide +ddATP mononucleotide mononucleotide trinucleotide trinucleotide hexanucleotide +ddCTP dinucleotide dinucleotide nonanucleotide hexanucleotide nonanucleotide +ddGTP octanucleotide octanucleotide decanucleotide decanucleotide (a) What base was altered and to which was it altered? Answer: From C V to A (b) What…

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Solved Attempt A DNA fragment was sequenced using dideoxy

Transcribed image text: Attempt A DNA fragment was sequenced using dideoxy sequencing. In this procedure, ddATP was tagged with a green dye, ddCTP was tagged with a blue dye, ddGTP was tagged with a yellow dye, and ddTTP was tagged with a red dye. The primer used had the sequence…

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