Tag: ddATP

Solved A researcher sequences a DNA fragment using

Transcribed image text: A researcher sequences a DNA fragment using dye-terminator sequencing. In this procedure, each dideoxynucleotide triphosphate (ddNTP) contains a fluorescent marker specific to the base. Upon excitement by a laser, the ddNTPs emit light at their characteristic wavelengths. An automated machine then reads the sequence. In this procedure,…

Continue Reading Solved A researcher sequences a DNA fragment using

Solved You Plan to sequence the following DNA by Sanger

Transcribed image text: You Plan to sequence the following DNA by Sanger sequencing. your reaction includes your sequencing primer (5−1 is on the (8t) and template DNA ( 5−1 end is on the left), dNIPs, buffer, DNA Polymerase and the following fluorescent dINTPs: red ddGTP, green ddATP, and blue ddITP….

Continue Reading Solved You Plan to sequence the following DNA by Sanger

Solved You are peforming a dideoxy chain termination

Transcribed image text: You are peforming a dideoxy chain termination sequencing reaction, and include the following components: 1. Template DNA inserted into a sequencing vector 2.Sequencing primers that anneal to the vector immediately upstream of the insertion site 3.ddATP, ddTTP.ddCTP, ddGTP fluorescently labeled with unique colors 4. DNA polymerase in…

Continue Reading Solved You are peforming a dideoxy chain termination

Normal With Genetic Disorder +ddTTP tetranucleotide

Transcribed image text: Normal With Genetic Disorder +ddTTP tetranucleotide tetranucleotide pentanucleotide pentanucleotide heptanucleotide heptanucleotide +ddATP mononucleotide mononucleotide trinucleotide trinucleotide hexanucleotide +ddCTP dinucleotide dinucleotide nonanucleotide hexanucleotide nonanucleotide +ddGTP octanucleotide octanucleotide decanucleotide decanucleotide (a) What base was altered and to which was it altered? Answer: From C V to A (b) What…

Continue Reading Normal With Genetic Disorder +ddTTP tetranucleotide

Solved Attempt A DNA fragment was sequenced using dideoxy

Transcribed image text: Attempt A DNA fragment was sequenced using dideoxy sequencing. In this procedure, ddATP was tagged with a green dye, ddCTP was tagged with a blue dye, ddGTP was tagged with a yellow dye, and ddTTP was tagged with a red dye. The primer used had the sequence…

Continue Reading Solved Attempt A DNA fragment was sequenced using dideoxy