Tag: DdCBE-mediated
Correction of a homoplasmic mitochondrial tRNA mutation in patient-derived iPSCs via a mitochondrial base editor
Human induced pluripotent stem cells (iPSCs) Reprogramming and Culture This study was ethically approved by the Medical Ethics Committee of Nanjing Maternal and Child Health Care Hospital (2021KY-131), and informed consents were obtained from the patient’s legal guardian as well as the healthy donors, in accordance with the Declaration of…
Single-cell individual full-length mtDNA sequencing by iMiGseq uncovers unexpected heteroplasmy shifts in mtDNA editing
doi: 10.1093/nar/gkad208. Online ahead of print. Chongwei Bi 1 , Lin Wang 1 , Yong Fan 2 , Baolei Yuan 1 , Gerardo Ramos-Mandujano 1 , Yingzi Zhang 1 , Samhan Alsolami 1 , Xuan Zhou 1 , Jincheng Wang 3 , Yanjiao Shao 4 , Pradeep Reddy 4 , Pu-Yao Zhang 5 , Yanyi Huang 3 6 , Yang Yu 5…
Shen Bin/Lou Xin team achieves efficient and accurate editing of zebrafish mitochondrial DNA
Mitochondrial diseases are a major genetic disease. The prevalence rate among newborns in the United States is about 1 in 5000. China currently lacks complete epidemiological data on mitochondrial diseases. At present, nearly 300 genes that cause mitochondrial diseases are known, including both mitochondrial DNA (mtDNA) coding and nuclear DNA…
