Tag: ddTTP

Solved A researcher sequences a DNA fragment using

Transcribed image text: A researcher sequences a DNA fragment using dye-terminator sequencing. In this procedure, each dideoxynucleotide triphosphate (ddNTP) contains a fluorescent marker specific to the base. Upon excitement by a laser, the ddNTPs emit light at their characteristic wavelengths. An automated machine then reads the sequence. In this procedure,…

Continue Reading Solved A researcher sequences a DNA fragment using

Solved You are peforming a dideoxy chain termination

Transcribed image text: You are peforming a dideoxy chain termination sequencing reaction, and include the following components: 1. Template DNA inserted into a sequencing vector 2.Sequencing primers that anneal to the vector immediately upstream of the insertion site 3.ddATP, ddTTP.ddCTP, ddGTP fluorescently labeled with unique colors 4. DNA polymerase in…

Continue Reading Solved You are peforming a dideoxy chain termination

Normal With Genetic Disorder +ddTTP tetranucleotide

Transcribed image text: Normal With Genetic Disorder +ddTTP tetranucleotide tetranucleotide pentanucleotide pentanucleotide heptanucleotide heptanucleotide +ddATP mononucleotide mononucleotide trinucleotide trinucleotide hexanucleotide +ddCTP dinucleotide dinucleotide nonanucleotide hexanucleotide nonanucleotide +ddGTP octanucleotide octanucleotide decanucleotide decanucleotide (a) What base was altered and to which was it altered? Answer: From C V to A (b) What…

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Solved Attempt A DNA fragment was sequenced using dideoxy

Transcribed image text: Attempt A DNA fragment was sequenced using dideoxy sequencing. In this procedure, ddATP was tagged with a green dye, ddCTP was tagged with a blue dye, ddGTP was tagged with a yellow dye, and ddTTP was tagged with a red dye. The primer used had the sequence…

Continue Reading Solved Attempt A DNA fragment was sequenced using dideoxy

SOLVED:DNA Sequencing A researcher used the Sanger method to sequence the DNA fragment shown. The red asterisk indicates a fluorescent label. She reacted a sample of the DNA with DNA polymerase and each of the four nucleotide mixtures (in an appropriate buffer) listed. Some of the mixtures included dideoxynucleotides (ddNTPs) in relatively small amounts. 1. dATP, dTTP, dCTP, dGTP, ddTTP 2. dATP, dTTP, dCTP, dGTP, ddGTP 3. dATP, dCTP, dGTP, ddTTP 4. dATP, dTTP, dCTP, dGTP The researcher then separated the resulting DNA by electrophoresis on a polyacrylamide gel and located the fluorescent bands on the gel. The image of the gel shows the band pattern resulting from nucleotide mixture 1 . Assuming that all mixtures were run on the same gel, what did the remaining lanes of the gel look like?

Problem 18 Easy Difficulty DNA Sequencing A researcher used the Sanger method to sequence the DNA fragment shown. The red asterisk indicates a fluorescent label. She reacted a sample of the DNA with DNA polymerase and each of the four nucleotide mixtures (in an appropriate buffer) listed. Some of the…

Continue Reading SOLVED:DNA Sequencing A researcher used the Sanger method to sequence the DNA fragment shown. The red asterisk indicates a fluorescent label. She reacted a sample of the DNA with DNA polymerase and each of the four nucleotide mixtures (in an appropriate buffer) listed. Some of the mixtures included dideoxynucleotides (ddNTPs) in relatively small amounts. 1. dATP, dTTP, dCTP, dGTP, ddTTP 2. dATP, dTTP, dCTP, dGTP, ddGTP 3. dATP, dCTP, dGTP, ddTTP 4. dATP, dTTP, dCTP, dGTP The researcher then separated the resulting DNA by electrophoresis on a polyacrylamide gel and located the fluorescent bands on the gel. The image of the gel shows the band pattern resulting from nucleotide mixture 1 . Assuming that all mixtures were run on the same gel, what did the remaining lanes of the gel look like?

‘The following diagram shows the result of the set of four DNA sequencing reactions by the Sanger-dideoxy method, using dideoxy analogs. What is the sequence of the template DNA? aaTp ddGTP ducIP ddTTP’

The Elements of Life In biology, the elements of life are the essential building blocks that make up living things. They are carbon, hydrogen, nitrogen, oxygen, phosphorus, and sulfur. The first four of these are the most important, as they are used to construct the molecules that are necessary to…

Continue Reading ‘The following diagram shows the result of the set of four DNA sequencing reactions by the Sanger-dideoxy method, using dideoxy analogs. What is the sequence of the template DNA? aaTp ddGTP ducIP ddTTP’