Tag: DESE2

Choosing the correct shrinkage type

Hi everyone, First time poster here – tried to look for the answer but I do not seem to find exactly what I am looking for. I have a question re log2FC shrinking as part of my DEse2 pipeline. I cannot understand which type of shrinkage I should be using….

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How to make a UMAP for single cell data and color cells by average expression of a list of genes in scanpy?

Hello, I have single cell and bulk RNA seq data for both of which I have performed some basic analysis. For the bulk RNA seq data I have performed DESe2 and I have gotten a list of DE genes. I would like to make a UMAP where the cells are…

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How to make a UMAP for single cell data and color cells by average expression of a list of cells in scanpy?

How to make a UMAP for single cell data and color cells by average expression of a list of cells in scanpy? 0 Hello, I have single cell and bulk RNA seq data for both of which I have performed some basic analysis. For the bulk RNA seq data I…

Continue Reading How to make a UMAP for single cell data and color cells by average expression of a list of cells in scanpy?

TPM value from DESE2 and significant filterig isssue

TPM value from DESE2 and significant filterig isssue 0 The code 10101 res_ddsDE_new has 36,000 rows. When I am using subset(res_ddsDE_new, padj < 0.05 & abs(log2FoldChange) > 1) res_ddsDE_new baseMean log2FoldChange <numeric> <numeric> DDX11L1 1.779144 -1.4955939 WASH7P 152.518293 -0.0505911 MIR6859-1 20.653876 0.5689275 MIR1302-2HG 0.255387 -1.9691031 FAM138A 0.353478 0.1574042 Then I…

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