Tag: DNA

Solved 2. Nucleic acids ( 5 points) a. In the Sanger

Transcribed image text: 2. Nucleic acids ( 5 points) a. In the Sanger (dideoxy) method for DNA sequencing, a small amount of dideoxynucleotides triphophosphate-say, ddCTP – is added to the sequencing reaction along with a larger amount of corresponding JCTP. i. What is the function of the ddCTP (i.e., why…

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PRC2 direct transfer from G-quadruplex RNA to dsDNA: Implications for RNA-binding chromatin modifiers

Abstract The chromatin-modifying enzyme, Polycomb Repressive Complex 2 (PRC2), deposits the H3K27me3 epigenetic mark to negatively regulate expression at numerous target genes, and this activity has been implicated in embryonic development, cell differentiation, and various cancers. A biological role for RNA binding in regulating PRC2 histone methyltransferase activity is generally…

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The Promise of RNA-Based Therapies

Published a day ago Submitted by CRB Decades of scientific progress in understanding the root cause of many formerly intractable diseases has opened up new treatment options, namely through RNA-based therapies. This second wave of biopharma, in which our understanding of the ways coding portions of our genome relate to…

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AWS launches Amazon Omics for precision medicine

To enhance clinical insights at the point of care and help identify the best treatment or prevention options for patients, Amazon Web Services has launched a service that utilizes artificial intelligence (AI), machine learning, and other AWS and partner products and services to run IT-heavy bioinformatics workflows.  WHY IT MATTERS…

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Some People More Prone to COVID-19 Vaccine Side Effects

Among individuals who have received COVID-19 vaccines, younger people, females, and those who got the second dose of the Moderna COVID-19 vaccine report more side effects from COVID-19 vaccines that may have led to moderate and severe limitations, according to a recent paper (Front Public Health 2022; doi: 10.3389/fpubh.2022.975781). These…

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dharmacon sirna design

Please feel free to visit our Privacy Policy and Cookie Policy with regards to processing your data. Article Customer Support Sequence characteristics of functional siRNAs. Human and mouse genes are each targeted with multiple shRNA constructs that have been cloned into the pGIPZ lentiviral vector. GRCF Management Team. In the…

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biology questions – hw 2.docx – 1) The red blood cells in your body are going through the process of transcription. What are the enzymes needed to make

1)The red blood cells in your body are going through the process of transcription. What arethe enzymes needed to make rRNA and the basic steps in which this process occurs?A)A ‘specific’ transcription factor binds to the promoter region and allows RNA PolymeraseI to read the DNA and create rRNA.B)A ‘specific’…

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Programmable CRISPR-Based Tool ‘Pastes’ in New Genes While Avoiding Double-Strand Breaks

NEW YORK — Researchers at MIT have developed a programmable tool that combines CRISPR and integrases to cut out unwanted stretches of DNA and paste in a replacement. While CRISPR-Cas9 gene editing can make such swaps, it is limited by the need for double-stranded DNA breaks, which can induce undesired…

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A CRISPR endonuclease gene drive reveals distinct mechanisms of inheritance bias

CRISPR/Cas gene drives can bias transgene inheritance through different mechanisms. Homing drives are designed to replace a wild-type allele with a copy of a drive element on the homologous chromosome. In Aedes aegypti, the sex-determining locus is closely linked to the white gene, which was previously used as a target…

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Phase 1 Trial to Test ‘Off-the-shelf’ CAR T-cell Therapy CB-011 in 2023 | CAR T-cell Therapy CB-011 to Be Tested in Hard-to-treat Myeloma in 2023

Caribou Biosciences will soon start testing its new CAR T-cell therapy candidate, CB-011, in people with relapsed or refractory multiple myeloma. A Phase 1 trial will begin enrolling eligible adults in early 2023 at multiple centers, according to the company. The announcement follows clearance of the company’s investigational new drug…

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Characterization, genome analysis and genetic tractability studies of a new nanocellulose producing Komagataeibacter intermedius isolate

Isolation, characterization and classification of BC-producing strain Isolation of single clones from CaCO3 halo zones in Glucose-Yeast Extract-Calcium carbonate agar and iterated subculturing in HS-Glu agar resulted in enrichment of an isolate with beige-coloured, smooth-edged and umbonate shaped colonies characteristics (Fig. S1A). The isolate is hereafter called ‘ENS15’. Under 100X magnification,…

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Unexpected absence of ribosomal protein genes from metagenome-assembled genomes

Hug LA, Baker BJ, Anantharaman K, Brown CT, Probst AJ, Castelle CJ, et al. A new view of the tree of life. Nat Microbiol. 2016;1:16048. Article  PubMed  Google Scholar  Castelle CJ, Wrighton KC, Thomas BC, Hug LA, Brown CT, Wilkins MJ, et al. Genomic expansion of domain archaea highlights roles…

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Versatile and efficient genome editing with Neisseria cinerea Cas9

PAM identification of NcCas9 by PAM-DOSE The type II-C Cas9 orthologue NcCas9 is of small size (1082 aa) and is closely related to conventional Neisseria meningitidis Cas9 (NmeCas9)11 (Fig. 1a). The NcCas9 is 94% identical to Nme1Cas9, and the divergences lie mainly in the C-terminal PAM-interacting domain (PID) (Fig. 1a and S1)….

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AI Tailors Artificial DNA for Future Drug Development

With the help of an AI, researchers at Chalmers University of Technology, Sweden, have succeeded in designing synthetic DNA that controls the cells’ protein production. The technology can contribute to the development and production of vaccines, drugs for severe diseases, as well as alternative food proteins much faster and at…

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Who are the leading innovators in DNA polymorphism detection for the pharmaceutical industry?

The pharmaceutical industry continues to be a hotbed of innovation, with activity driven by the evolution of new treatment paradigms, the gravity of unmet need, as well as the growing importance of technologies such as pharmacogenomics, digital therapeutics, and artificial intelligence (AI). In the last three years alone, there have…

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New CRISPR gene-editing system can “drag-and-drop” DNA in bulk

A new technique has been added to the CRISPR gene-editing toolbox. Known as PASTE, the system uses virus enzymes to “drag-and-drop” large sections of DNA into a genome, which could help treat a range of genetic diseases. The CRISPR system originated in bacteria, which used it as a defense mechanism…

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Community succession and functional prediction of microbial consortium with straw degradation during subculture at low temperature

Changes of straw degradation characteristics at different culture stages Corn straw degradation ratio Corn straw weight loss in M44 at F1 reached 35.90% at 15 ℃ for 21 days, which was greater than that at F5, F8, and F11 by 2.33%, 3.01%, and 3.35%, respectively. There were no significant differences between…

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A Beginner’s Guide to Microbial Shotgun Metagenomic Sequencing

A Beginner’s Guide to Microbial Shotgun Metagenomic Sequencing Shotgun metagenomic sequencing offers information on the complete genomic DNA of all organisms in a sample without the need for microorganism isolation and cultivation or target regions amplification. This is crucial since almost 99 percent of all microorganisms are thought to be…

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Solved What is the major factor in stabilizing dsDNA

Transcribed image text: What is the major factor in stabilizing dsDNA (double-stranded DNA)? [2 points] Consider the statement “pure hydrocarbons do not form monolayers on water”. Provide an explanation for this statement [ 2 points] Consider the following pairs of fatty acids and underline the fatty acid in each pair…

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scRNA-seq for Microcephaly Research [I]: Single-Cell Droplet Encapsulation, mRNA Capture, and cDNA Synthesis

doi: 10.1007/978-1-0716-2752-5_8. Affiliations Expand Affiliations 1 Department of Medicine, Division of Immunology, Lowance Center for Human Immunology, Emory University School of Medicine, Atlanta, GA, USA. ben.babcock@emory.edu. 2 Department of Neurology, University of North Carolina Medical School, Chapel Hill, NC, USA. Item in Clipboard Benjamin Babcock et al. Methods Mol Biol. 2023….

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I want to identify the version of MethylationEPIC from the idat file.

I want to identify the version of MethylationEPIC from the idat file. 1 I have idat files of Infinium MethylationEPIC. I would like to know if the array of this file is Infinium MethylationEPIC v1.0 or Infinium MethylationEPIC v2.0. Is it possible to investigate the version of the MethylationEPIC from…

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Liver Cancers Detected With Parallel Mutation, Methylation Liquid Biopsy Approach

NEW YORK – A Chinese research team has shown that their method for profiling mutation and DNA methylation patterns in circulating cell-free DNA (cfDNA) can detect hepatocellular carcinoma (HCC) liver cancer cases. “[W]e developed the Mutation Capsule Plus (MCP) technology, supporting parallel profiling of mutations and methylation changes (MCP profiling)…

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Dementia with Lewy bodies post-mortem brains reveal differentially methylated CpG sites with biomarker potential

Weisman, D. & McKeith, I. Dementia with Lewy Bodies. Semin. Neurol. 27, 042–047 (2007). Article  Google Scholar  Foguem, C. & Manckoundia, P. Lewy Body Disease: Clinical and Pathological “Overlap Syndrome” Between Synucleinopathies (Parkinson Disease) and Tauopathies (Alzheimer Disease). Curr. Neurol. Neurosci. Rep. 2018 18:5 18, 1–9 (2018). CAS  Google Scholar …

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Twist Bioscience Gets Stung by Short Report

Someone once said it’s easier to fool people than convince them they’ve been fooled. For those who invest with conviction, nothing damages one’s pride more than accusations of being conned. Investors must be wary of letting such emotions get in the way of objectivity. Anyone who dismisses an entire short…

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BBC’s jab travesty and the critics who will not be fobbed off

HAS the BBC’s complaints system finally come up against a foe that won’t be put off? A brief history of complaints about the travesty of a BBC documentary, Unvaccinated, proudly promoted in July, suggests it may well have.  The programme, presented by Professor Hannah Fry, signalled its bias from the start. Tom Coveney,…

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Rutgers scientists develop new approach to effectively shut down viral infections

Rutgers scientists have developed a new approach to stopping viral infections: a so-called live-attenuated, replication-defective DNA virus vaccine that uses a compound known as centanamycin to generate an altered virus for vaccine development. The method was tested to produce a weakened or “attenuated” version of a mouse cytomegalovirus, a common…

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Bioinformatics Analyst II – Epigenetics & 3D Genomics Job in Texas

Summary The lab of Dr. Kyle Eagen (www.eagenlab.org) at Baylor College of Medicine focuses on elucidating how DNA folds within cells and how DNA misfolding relates to human disease. Baylor College of Medicine, one of the top ranked medical schools in the country, is well known for its exceptional,…

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“DNA Virus Vaccine” Created To Fight DNA Viruses

Rutgers scientists have developed a new approach to stopping viral infections: a so-called live-attenuated, replication-defective DNA virus vaccine that uses a compound known as centanamycin to generate an altered virus for vaccine development.   The method was tested to produce a weakened or “attenuated” version of a mouse cytomegalovirus, a…

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RNA vaccines rose to prominence during COVID, but we’ve only scratched the surface of their potential

In high school biology, students are taught the central concept of genetics in a very simple way: DNA makes RNA, RNA makes protein, and protein makes life. In this way, RNA is central to everything in biology — and, you could argue, central to life itself. Biologically, DNA is the…

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P. falciparum sequencing

P. falciparum sequencing 1 I am planning an experiment to sequence and analyse Plasmodium falciparum samples for resistance gene detection. Are there any peculiarities to take note of given the high AT richness of the Plasmodium falciparum genome? Also, can anyone recommend sequencing kits for this type of experiment? AT-rich…

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Inovio Pharmaceuticals Stock Up By 28% In The Last 21 Sessions

(VIANEWS) – Shares of Inovio Pharmaceuticals (NASDAQ: INO) rose by a staggering 28.66% in 21 sessions from $1.64 at 2022-10-20, to $2.11 at 14:28 EST on Friday, after two sequential sessions in a row of losses. NASDAQ is falling 0.39% to $11,101.66, after two successive sessions in a row of…

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Induction of Genital Immunity by DNA Priming and Intranasal Booster Immunization with a Replication-Defective Adenoviral Recombinant1 | The Journal of Immunology

Mice immunized through different routes such as i.m., intradermally, or intratracheally with a DNA vaccine to rabies virus developed high titers of serum Ab but only borderline levels of mucosal Abs determined from vaginal secretions. DNA vaccines given by either route enhanced vaginal IgA and IgG2a secretion upon a subsequent…

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Polyethylene glycol PEG mediated transformation Plant protoplasts plant cells

7ElectroporationThe Electroporation of cells can be used to deliver DNA into plant cells andprotoplasts. The vectors used can be simple plasmids. The genes of interest require plantregulatory sequences, but no specific sequences are required for integration. Material isincubated in a buffer solution containing DNA and subjected to high-voltage electricalpulses. The…

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B.Tech Bioinformatics Jobs, Scope, Salary in India 2022

B.Tech Bioinformatics is a course that gives the students a variety of job roles. The private and public sectors are demanding for B.Tech Bioinformatics Graduates. Some of the job roles of B.Tech Bioinformatics Graduates include; Research Scientists, Bioinformatics software developers, Bioinformaticians, Laboratory Assistants, Teaching Assistants, Clinical Research coordinators, Bioinformatics Analyst,…

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Rutgers scientists produce “DNA virus vaccine

Rutgers scientists have developed a new approach to stopping viral infections: a so-called live-attenuated, replication-defective DNA virus vaccine that uses a compound known as centanamycin to generate an altered virus for vaccine development.   The method was tested to produce a weakened or “attenuated” version of a mouse cytomegalovirus, a…

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IMUNON Announces Strategic Investment in Transomic Technologies

Investment of $375,000 with Senior Convertible Notes and Warrants; Strengthens Development Capabilities for IMUNON’s PLACCINE DNA Vaccine Platform IMUNON’s Executive Chairman to join Transomic’sBoard of Directors LAWRENCEVILLE, N.J., Nov. 17, 2022 (GLOBE NEWSWIRE) — IMUNON, Inc. (NASDAQ: IMNN), a clinical-stage company focused on DNA-based immunotherapy and next-generation…

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Research Investigator – Dr. Karras’s Lab (Bioinformatics & Genetics) job with University of Texas MD Anderson Cancer Center

The University of Texas MD Anderson Cancer Center in Houston is one of the world’s most respected centers focused on cancer patient care, research, education and prevention. It was named the nation’s No. 1 hospital for cancer care in U.S. News & World Report’s 2022 rankings. It is one of…

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Evaxion announces promising results from Phase 1/2a clinical trial of personalized DNA cancer immunotherapy EVX-02 | Vaccines

Evaxion announces promising results from Phase 1/2a clinical trial of personalized DNA cancer immunotherapy EVX-02 Details Category: Vaccines Published on Thursday, 17 November 2022 15:54 Hits: 245 COPENHAGEN, Denmark I November 17, 2022 I Evaxion Biotech A/S (NASDAQ: EVAX) (“Evaxion” or the “Company”), a clinical-stage biotechnology company specializing in the…

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CRISPR/Cas editing tools

Sponsored Content by GenScriptNov 16 2022Reviewed by Maria Osipova In a new application for CRISPR/Cas editing tools, researchers at Harvard Medical School, Boston, Massachusetts, have created a CRISPR/Cas9 ribonucleoprotein-based peptide display approach. This technique is hoped to simplify the peptide library analysis workflow by using a new technique called peptide immobilization…

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Scientist, Bioinformatics Job in Carlsbad, CA – Jobot

Scientist, Bioinformatics – ChIP-seq, RNA-seq, Hi-C, ATAC-seq, WGS, Exome-seq This Jobot Job is hosted by: Emmet NittoAre you a fit? Easy Apply now by clicking the “Apply Now” button and sending us your resume.Salary: $90,000 – $115,000 per year A bit about us: Based in Carlsbad, CA, we are a…

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Novavax vaccine for COVID-19 | Waushara Argus

Currently, according to the CDC, there are three main types of COVID-19 vaccines that are approved or authorized for use in the United States: mRNA, viral vector, and protein subunit. Each type of vaccine prompts our bodies to recognize and help protect us from the virus that causes COVID-19. None…

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Global DNA Sequencing Market strategy patterns and forecasts in the coming years 2033

Pune, Maharashtra, India, November 15 2022 (Wiredrelease) Mrfactors.com –:The Global DNA Sequencing Market is estimated to be USD 4581.2 million in 2022 and is expected to reach USD 16915.22 million by 2033, growing at a CAGR of 11.5%. DNA sequencing is the process of determining a DNA segment’s nucleotide sequence….

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mRNA vaccines against COVID-19 don’t modify humans, and don’t make them patentable or owned by any entities

CLAIM “if a human is injected with a GMO it becomes a patented piece of property from the government”, “they take away all your rights with mRNAs” DETAILS Factually inaccurate: U.S. law prohibits the patenting of human beings. Furthermore, mRNA vaccines don’t modify the human genome. Inadequate support: The 2013…

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India producing medical devices at 1/3rd world price, says Union Minister Jitendra Singh

New Delhi [India], November 16 (ANI): Union Minister for Science and Technology Jitendra Singh on Tuesday said India is among the top five countries in the world manufacturing life-saving high-risk medical devices but the cost of our devices is about one-third of those manufactured by the other four countries. Addressing…

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Research Associate, Bioinformatics (Cancer Science Institute, NUS) job with NATIONAL UNIVERSITY OF SINGAPORE

Job Description A Bioinformatics position is available immediately at the Cancer Science Institute (a part of The National University of Singapore) in the laboratory of Dr. Sriram Sridharan. The research will focus in the broad area of replication stress, DNA damage and genome integrity.  This is an exciting opportunity to…

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Extra-hematopoietic immunomodulatory role of the guanine-exchange factor DOCK2

Cell isolation, reprogramming and culture Approval was obtained for human cell and tissue sample collection and genetic reprogramming from the Institutional Review Board (protocols 19–252, 18–243, 21–060, 19–284 and 415-E/1776/4-2014, Ethics Committee of the province of Salzburg). Adult samples were collected in accordance with the Declaration of Helsinki after written…

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IMUNON, INC. MANAGEMENT’S DISCUSSION AND ANALYSIS OF FINANCIAL CONDITION AND RESULTS OF OPERATIONS. (form 10-Q)

The following discussion and analysis of our financial condition and results of operations This discussion contains forward-looking statements that involve risks and uncertainties. Our actual results may differ materially from those discussed in forward-looking statements. Factors that might cause a difference include, but are not limited to, those discussed above…

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Inovio Pharmaceuticals Stock Up Momentum With A 31% Rise In The Last 21 Sessions

(VIANEWS) – Shares of Inovio Pharmaceuticals (NASDAQ: INO) rose by a staggering 31.69% in 21 sessions from $1.83 at 2022-10-17, to $2.41 at 11:43 EST on Monday, following the last session’s downward trend. NASDAQ is falling 0.67% to $11,247.78, after two successive sessions in a row of gains. Inovio Pharmaceuticals’s…

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“Seminal” study gene edits immune cells to target cancer

In a new study described by one oncologist as “seminal” and “pioneering”, researchers in the United States have successfully used CRISPR gene-editing technology to create personalized cancer-targeting immune cells. In a Phase 1 trial the researchers tested the technology on 16 patients, and while it was only mildly effective in…

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CAS9 Technology Market is Set to Experience a Revolutionary Growth during 2022-2032 | Cellecta, Inc., abm Inc., GenScript, CRISPR Therapeutic, Intellia Therapeutics, Inc., GE Healthcare, Takara Bio, Inc., AstraZeneca

Scope of CAS9 Technology Market The Global CAS9 Technology Market 2032 Industry Report is a professional and in-depth study on the current state of the CAS9 Technology Market by QMI. The CAS9 Technology Market is supposed to demonstrate a considerable growth during the forecast period of 2022 – 2032. The company…

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DNA is providing new clues to why COVID-19 hits people differently | National News | San Antonio

click to enlarge UnSplash / Jacek Pobłocki Researchers are looking at a wide variety of factors that may play a role in how sick people become from COVID-19. Since the beginning of the pandemic, the mercurial nature of the coronavirus has been on display. Some people get mild, cold-like illnesses…

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A potent, broadly protective vaccine against SARS-CoV-2 variants of concern

Cells and viruses hACE2 expressing BHK-21 (BHK-21-hACE2) and Vero-E6 cells were provided by State Key Laboratory of Virology, Wuhan University. Vero-E6 cells were cultured in DMEM (Gibco, Cat.11995–040) supplemented with 10% fetal bovine serum (Gibco, Cat.10091–148) and 1% penicillin and streptomycin solution (BasalMedia, Cat.S110JV) at 37°C with 5% CO2. BHK-21-hACE2…

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Single-cell transcriptome analysis reveals cellular heterogeneity in mouse intra- and extra articular ligaments

Amiel, D., Frank, C., Harwood, F., Fronek, J. & Akeson, W. Tendons and ligaments: a morphological and biochemical comparison. J. Orthop. Res. 1, 257–265 (1984). PubMed  CAS  Google Scholar  Lipps, D. B., Wojtys, E. M. & Ashton-Miller, J. A. Anterior cruciate ligament fatigue failures in knees subjected to repeated simulated…

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re-annotation of enhancers and long noncoding RNA genes and benchmarking of normalization methods

Illumina Infinium DNA Methylation (5mC) arrays are a popular technology for low-cost, high-throughput, genome-scale measurement of 5mC distribution, especially in cancer and other complex diseases. After the success of its HumanMethylation450 array (450k), Illumina released the MethylationEPIC array (850k) featuring increased coverage of enhancers. Despite the widespread use of 850k,…

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Mobile resistome of microbial communities and antimicrobial residues from drinking water supply systems in Rio de Janeiro, Brazil

Ilyas, M. et al. Environmental and health impacts of industrial wastewater effluents in Pakistan: A review. Rev. Environ. Health 34, 171–186 (2019). MathSciNet  PubMed  CAS  Google Scholar  Roy, M. A. et al. A Metagenomic approach to evaluating surface water quality in Haiti. Int. J. Environ. Res. Public Health 15, 2211…

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Digital Genome Market Projected to Reach US$ 20,812.81 million in 2027

According to a new market research study of “Digital Genome Market to 2027 – Global Analysis and Forecast by Product, Application, and End User,” the global digital genome market is expected to reach US$ 20,812.81 million in 2027 from US$ 11,065.31 million in 2019. The market is estimated to grow…

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High-Quality Genome of Pearl Oysters Constructed

Pearl oysters are an important aquaculture animal in Japan, as they produce the beautiful pearls that are sought after for necklaces, earrings, and rings. In the early 1990s, this aquaculture industry was bringing in around 88bn yen annually. But, in the last 20 years, a combination of new diseases and…

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3D protein structure predictions made by AI could boost cancer research and drug discovery

The space of characteristic structural elements in AF2 structural models for 21 species. Visualization of t-SNE dimensionality reduction analysis, in which structures with similar structural elements are placed closer together and the 20 most common superfamilies are colored. The axes corresponding to the t-SNE dimension 1 and t-SNE dimension 2…

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OncoSec Announces Reverse Stock Split

PENNINGTON, N.J. and SAN DIEGO, Nov. 8, 2022 /PRNewswire/ — OncoSec Medical Incorporated (NASDAQ: ONCS) (the “Company” or “OncoSec”), a clinical-stage biotechnology company focused on developing intratumoral immunotherapies to stimulate the patient’s own immune system to target and eradicate cancer, today announced it intends to effect a 1-for-22 reverse split…

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Researchers develop new machete technique to slice into cancer genome and study copy number alterations — ScienceDaily

MACHETE is a new CRISPR-based technique developed by researchers at the Sloan Kettering Institute (SKI) to study large-scale genetic deletions efficiently in laboratory models. People are already calling it the Machete Paper. Still, lead authors Francisco “Pancho” Barriga and Kaloyan Tsanov of the Sloan Kettering Institute don’t want the name…

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COVID-19 Vaccine Induced Thrombotic Thrombocytopenia

There are over 1000 peer-reviewed papers in the preprint server system and or in the National Library of Medicine (PUBMED) describing side effects after mRNA or adenoviral DNA COVID-19 vaccination.  One of the most dreaded complications is vaccine-induced thrombotic thrombocytopenia (VITT).   Because the Spike protein produced in response to the…

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Rhythm Pharmaceuticals Announces Publication of Results

— Previously disclosed data demonstrated statistically significant and clinically meaningful reductions in weight and hunger in patients with Bardet-Biedl syndrome — BOSTON, Nov. 08, 2022 (GLOBE NEWSWIRE) — Rhythm Pharmaceuticals, Inc. (Nasdaq: RYTM), a commercial-stage biopharmaceutical company focused on transforming the lives of patients and their families living with hyperphagia…

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Solved Which of the following statements about PCR is FALSE?

Transcribed image text: Which of the following statements about PCR is FALSE? Check all that apply. PCR uses a heat-stable DNA polymerase. After every cycle of replication, there is a linear increase in the amount of DNA present. Every round of replication starts with the denaturation of dsDNA molecules. If…

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a database of DNA repair pathways

Protein FULL name: CPD photolyase, DNA photolyase Phr (Escherichia coli strain K-12 substr. MG1655) is product of expression of phr gene. Phr is involved in: DDS in Escherichia coli strain K-12 substr. MG1655 DRR in Escherichia coli strain K-12 substr. MG1655 Keywords: FUNCTION: Involved in repair of UV radiation-induced DNA…

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cell Large Granular Lymphocytic Leukemia

Large granular lymphocyte leukemia (LGLL) is a rare chronic lymphoproliferative disorder originating in the mature T-cells and natural killer (NK) cells, accounting for 2% to 5% of all the chronic lymphoproliferative disorders in North America and Europe. T-cell LGLL (T-LGLL) is the most common subtype representing about 85% of LGLL…

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Mitochondrial myopathies due to mtDNA muts are inherited solely in a maternal

Mitochondrial myopathies due to mtDNAmuts are inherited solely in a maternalfashion (i.e. maternal inheritence).Therefore, transmission occurs only thruaffected females and never thru males.Mitochondrial myopathy• CNS and skeletal muscles affected first• Weakness,lactic acidosis, ragged redfibers, neuropathy, seizures• Maternal inheritance xLinkeddominant• Rett, Alport, Fragile X• All female children of affect males willhave…

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Simulated global warming affects endophytic bacterial and fungal communities of Antarctic pearlwort leaves and some bacterial isolates support plant growth at low temperatures

Sample collection Colobanthus quitensis samples were collected at King George Island near the Henryk Arctowski Polish Antarctic Station, Maritime Antarctica (62°14’ S, 58°48’ W) during the summer season (February 2018). Samples were collected inside the Antarctic Specially Protected Area (ASPA) 128 using permits provided by The Chilean Antarctic Institute (INACH)…

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Meta’s new AI just predicted the shape of 600 million proteins in 2 weeks

Scientists at Meta, the parent company of Facebook and Instagram, have used an artificial intelligence (AI) language model to predict the unknown structures of more than 600 million proteins belonging to viruses, bacteria and other microbes. The program, called ESMFold, used a model that was originally designed for decoding human…

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Technical Program Manager, Bioinformatics at Twist Bioscience

Sr. Technical Program Manager, Bioinformatics  Twist’s Program Management Office (PMO) is seeking an innovator, leader, and problem solver to enable the tremendous growth of Twist’s Next Generation DNA synthesis business. As a Technical Program Manager, you will be based within the PMO but will partner closely with bioinformatics and R&D…

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17-Year-Old Indian American Student Becomes First To Sequence Angelfish DNA

Indeever Madireddy is an avid fish-keeper and biologist. Indeever Madireddy, an Indian American student studying at BASIS Independent Silicon Valley, a private school at the Silicon Valley has become the first person to sequence the genome of freshwater angelfish. He did the sequencing after his pet fish died in March…

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Equities Analysts Set Expectations for CRISPR Therapeutics AG’s FY2022 Earnings (NASDAQ:CRSP)

CRISPR Therapeutics AG (NASDAQ:CRSP – Get Rating) – Investment analysts at William Blair cut their FY2022 EPS estimates for shares of CRISPR Therapeutics in a research report issued on Tuesday, November 1st. William Blair analyst R. Prasad now anticipates that the company will earn ($8.76) per share for the year,…

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Could a Breast Cancer Vaccine Treat, Prevent the Disease?

According to a recent press release from the University of Washington, preliminary research findings suggest that a vaccine could potentially treat breast cancer. Clinical trials generated a strong immune response to a key tumor protein, according to researchers of UW of Medicine in Seattle. “Because this was not a randomized clinical…

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CVC Files Appeal Brief in Interference No. 106,115 | McDonnell Boehnen Hulbert & Berghoff LLP

The Patent Trial and Appeal Board (like its predecessor, the Board of Patent Appeals and Interferences or BPAI) occasionally renders an opinion having the tendency to raise an eyebrow or two, which on occasion has led the Federal Circuit, like its predecessor the Court of Claims and Patent Appeals, to…

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Vaccine Generates Immune Response Against Breast Cancer in Trial

Researchers in a new study describe the development of an experimental vaccine that could be used to prevent or treat an aggressive form of breast cancer, safely generating an immune response against an important driver of breast cancer growth. “Because this was not a randomized clinical trial, the results should…

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Metagenomics | SpringerLink

This volume provides up-to-date and novel techniques for various screening technologies currently used in metagenomics and related areas. Starting with DNA/RNA isolation from environmental samples, the book continues by delving into areas such as current methods used to isolate DNA and construct metagenomic libraries, establishment of metagenome libraries in non-E….

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Low Prevalence of Anti-TNF-Induced Lupus in Patients With Inflammatory Bowel Disease

The incidence and clinical and serologic characteristics of anti-tumor necrosis factor (TNF)-induced lupus (ATIL) in patients with inflammatory bowel disease (IBD) were evaluated in a systematic review published in International Immunopharmacology. Researchers used PubMed, Ovid Embase, Medline, and Cochrane CENTRAL databases from inception through April 2022 for studies describing ATIL…

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Multiplexed base editing through Cas12a variant-mediated cytosine and adenine base editors

Robust C-to-T conversion by Cas12a variant-mediated CBEs for non-canonical PAMs We initially attempted to screen for optimal cytosine deaminases that confer powerful C-to-T editing for Cas12a-mediated CBEs. Seven dCas12a-CBEs fused with individual cytosine deaminases1,2,16,20,21,22,24,25,26 were compared in HEK293T cells and porcine embryos at sites with TTTV PAM sequences. The dCas12a-A3A…

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Twist Bioscience, Illumina Partner to Produce Exome Panel for Disease Research

NEW YORK – Twist Bioscience said on Tuesday that it has inked a partnership with Illumina to produce the co-branded Twist Bioscience for Illumina Exome 2.0 Plus panel for disease research. Under the partnership, Twist will manufacture the Exome 2.0 Plus panel, which can be used on Illumina sequencers and…

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Making glioblastoma more vulnerable to treatm

image: Dr. Waaqo Daddacha view more  Credit: Michael Holahan, Augusta University In the tough war against glioblastoma, scientists are taking a cue from viruses on how to make the aggressive cancer more vulnerable to treatment. Their target is SAMHD1, a protein which can protect us from viral infections by destroying an…

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Synthetic Biology Market Set for Rapid Growth 2031

Synthetic Biology Market Outlook 2031 The global synthetic biology market was valued at US$ 10.7 Bn in 2021 The global market is projected to advance at a CAGR of 21.3% from 2022 to 2031 The global synthetic biology market is anticipated to reach more than US$ 74.7 Bn by the end of 2031 Synthetic Biology Market Introduction Synthetic biology is a branch of science…

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Independent Longitudinal Study Finds CareDx’s AlloSure Detects Early Signs of Rejection in Pediatric Kidney Transplant Patients

First Pediatric Study to Evaluate Use of AlloSure dd-cfDNA as Biomarker for Allograft BK Infection Findings Add to Growing Body of Evidence Showing Clinical Value of AlloSure for Surveillance in Pediatric Kidney Transplant Patients BRISBANE, Calif.–(BUSINESS WIRE)– CareDx, Inc. (Nasdaq: CDNA) – The Transplant Company™ focused on the discovery, development, and…

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Myriad Genetics to Submit Hereditary Cancer Risk Variants to ClinVar in 2023

NEW YORK – Myriad Genetics will begin submitting variants detected by its hereditary cancer risk test, including variants in BRCA1 and BRCA2 genes, to the public database ClinVar starting in the spring of 2023. The Salt Lake City-based company is infamous, derided, and even boycotted in certain circles in the…

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Sales of Forensic Technologies Market 2028

Forensic Technologies Market: Introduction According to the report, the global forensic technologies market was valued at US$ 51.6 Bn in 2020 and is projected to expand at a CAGR of 13.2% from 2021 to 2028. The forensic technology refers to the scientific system of searching, investigating, retrieving, and analyzing the evidence gathered…

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Novel equi merozoite antigen (ema-1) gene heterogeneity in a geographically isolated Theileria equi population in Croatia | Parasites & Vectors

Richter S. Vrste haemosporidia u konja i goveda NR Hrvatskoj. Thesis: Veterinary faculty university of zagreb; 1954. Google Scholar  Rothschild CM. Equine piroplasmosis. J Equine Vet Sci. 2013;33:497–508. Article  Google Scholar  Ueti MW, Palmer GH, Scoles GA, Kappmeyer LS, Knowles DP. Persistently infected horses are reservoirs for intrastadial tick-borne transmission…

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Forensic Science Mitochondrial DNA Analysis.pdf – What are the advantages and disadvantages of mitochondrial DNA analysis? Could a police officer take a

What are the advantages and disadvantages of mitochondrial DNA analysis? Could apolice officer take a DNA sample from a suspect arrested for a serious crime? Explain.Mitochondrial DNA analysis is one of the tools that crime scene investigators use tosolve crimes. In this essay I will define mitochondrial DNA analysis, discuss…

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Leveraging Fermentation To Produce Complex APIs

Historically, plants and medicinal herbs have been used for centuries as remedies for ailments such as the common cold, nausea and skin conditions, as well as to boost digestion and support the immune system. Following decades of research leveraging the diverse chemicals that plants produce allow for the development of…

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The Next Generation of COVID-19 Vaccines and Boosters

The US Food and Drug Administration (FDA) emergency use authorization (EUA) for the first vaccines combating COVID-19 was a milestone in the 2020 pandemic. Due to this provision, Pfizer-BioNTech’s BNT162b2 (Comirnaty), Moderna’s mRNA-1273 (Spikevax), and Johnson & Johnson/Janssen’s COVID-19 vaccine (Ad26.COV2.S) received EUAs for COVID-19 prevention in December 2020, February…

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Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts

We designed pDOC to support multiple strategies for analyzing protein degradation in vitro, specifically cell-free systems. The device is based on a MITOMI integrated microfluidic chip, originally developed to quantify protein-ligand interactions at equilibrium24,33. The MITOMI module was modified to contain an array of 16 × 64, 32 × 32…

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Cellular glycan modification by B3GAT1 broadly restricts influenza virus infection

Ethics statement All procedures involving laboratory mice were approved by the Duke University IACUC under the protocol numbers A189-18-08 and A142-21-07. Mice were housed with up to five mice per cage and the ambient room conditions ranged from 70–74 °F and 30–70% humidity with a 12-hour dark/light cycle. Animals were assessed…

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Targeted co-delivery of paclitaxel and anti P-gp shRNA by low molecular weight PEI decorated with L-3,4-dihydroxyphenylalanine

doi: 10.1002/btpr.3310. Online ahead of print. Affiliations Expand Affiliations 1 Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran. 2 Department of Pharmaceutics, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran. 3 Center for Nanotechnology in Drug Delivery, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz,…

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Multicancer Early Detection Feasible for Outpatients

A blood-based multicancer early detection (MCED) test using cell-free DNA (cfDNA) is feasible for outpatients and has a positive predictive value of about 40%, according to a study presented at the 2022 annual meeting of the European Society for Medical Oncology. Deb Schrag, MD, MPH, and colleagues examined the clinical…

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Genomic signatures associated with maintenance of genome stability and venom turnover in two parasitoid wasps

Genomic features of two Anastatus wasps, A. japonicus and A. fulloi We employed PacBio high-fidelity (HiFi) long-read sequencing and Illumina short-read sequencing technologies to generate high-quality contigs for two Anastatus wasps, A. japonicus and A. fulloi (Supplementary Tables 1 and 2). These contigs were further scaffolded using Hi-C libraries to…

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Droplet microfluidics-based high-throughput bacterial cultivation for validation of taxon pairs in microbial co-occurrence networks

Conception of the workflow to demonstrate the microbial associations from co-occurrence networks with microbial cultivation Microbial co-occurrence networks are composed of nodes and edges, which usually represent microbes and statistically significant associations between microbes, respectively. We hypothesized that the microbial associations could be validated if the topological properties of networks…

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Algorithm helps in massive global effort to develop a more complete human reference genome

October 27, 2022October 27, 2022 A Clemson University professor has found himself at the heart of a monumental, international effort to sequence the human reference genome. Feng Luo, the Marvin J. Pinson, Jr. ’46 Distinguished Professor in Clemson’s School of Computing, helped develop an algorithm used in the project’s…

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Restriction Endonucleases Products (REP) Market Business Overview and Global Development Trends

Global Restriction Endonucleases Products (REP) Market: Overview The leading applications for the products and solutions offered by the manufacturers and players functional in the landscape of the global restriction endonucleases products (REP) market include epigenetics, fragment length polymorphism or RFLP, cloning, sequencing, polymerase chain reaction, and restriction digestion, among others….

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Evasion of cGAS and TRIM5 defines pandemic HIV

Cells and reagents HEK293T and U87 cells were maintained in DMEM medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Labtech) and 100 U ml−1 penicillin plus 100 μg ml−1 streptomycin (Pen/Strep; Gibco). THP-1-IFIT1 cells that had been modified to express Gaussia luciferase under the control of the IFIT1 promoter were described previously62. THP-1…

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Cas9 targeted nanopore sequencing with enhanced variant calling improves CYP2D6-CYP2D7 hybrid allele genotyping

Please use this url to cite or link to this publication: hdl.handle.net/1854/LU-8771189 MLA Rubben, Kaat, et al. “Cas9 Targeted Nanopore Sequencing with Enhanced Variant Calling Improves CYP2D6-CYP2D7 Hybrid Allele Genotyping.” PLOS Genetics, edited by Guanzheng Luo, 2022, doi:10.1371/journal.pgen.1010176. APA Rubben, K., Tilleman, L., Deserranno, K., Tytgat, O., Deforce, D., &…

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Phenotypic plasticity and genetic control in colorectal cancer evolution

Sample preparation and sequencing The method of sample collection and processing is described in a companion article (ref. 23). Sequencing and basic bioinformatic processing of DNA-, RNA- and ATAC-seq data are included there as well. Gene expression normalization and filtering The number of non-ribosomal protein-coding genes on the 23 canonical chromosome pairs…

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Tiny crustacean reveals deepest parts of oceans like isolated islands

A tiny crustacean measuring just three centimetres in length is helping scientists unlock one of the most perplexing questions of the oceans’ hadal zone–how isolated are the animals that live in our deepest ocean trenches? A new paper Barriers to gene flow in the deepest ocean ecosystem: Evidence from global population…

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