Tag: DRG

Deseq2 output and robust PCA (PcaGrid and PcaHubert)

Deseq2 output and robust PCA (PcaGrid and PcaHubert) 0 Hello there, I followed the deseq2 file to get the list of differentially expressed genes of a dataset I am working on. The Pca graph I got was not enough to show which one of the replicate is an outlier. A…

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DNA methylation change in blood cells of FB and CFS patients

Introduction Fibromyalgia (FM) and Chronic Fatigue Syndrome (CFS) are characterized by chronic pain, fatigue, and weakness. Patients with these symptoms also suffer from sleep abnormalities and report affected cognitive processes such as memory. The diagnosis of these two syndromes is challenging and is based on questionnaires that make the diagnosis…

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Impact of germline DNA repair gene variants on prognosis and treatment of men with advanced prostate cancer

In this study, we demonstrate an independent effect of germline DNA repair gene variants on the outcomes of patients with advanced PC. Despite carrier numbers being limited, we were able to identify germline DRG carrier status as an independent prognostic factor for PFS on first-line ARSI treatment, time to progression…

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Total Flavonoids of Rhizoma Drynariae Treat Osteoarthritis

Introduction Osteoarthritis (OA) is a common degenerative joint disease characterized by chronic pain and ambulation limitation, affecting about 250 million people worldwide.1 With increased aging population, the OA incidence rapidly increases and OA has become a major cause of disability.2,3 The morbidity of OA is closely related to articular cartilage,…

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Aedes aegypti Argonaute 2 controls arbovirus infection and host mortality

Generation and characterization of Ago2 knockout mutants To demonstrate the essential function of Ago2 in the Ae. aegypti siRNA pathway, we used CRISPR/Cas9 to knock out Ago2 and then investigated its role in defending against arbovirus infection (Fig. 1a). Ae. aegypti Ago2 (AeAgo2) has four exons and four predicated functional domains,…

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wwPDB News: PDB entries with new ligands only in PDBx/mmCIF format

At current growth rates, we anticipate running out of three-character Chemical Component IDs by the end of 2023. After this point, the wwPDB will issue five-character alphanumeric accession codes for CCD IDs in the OneDep system. To avoid confusion with current four-character PDB IDs, four-character codes will not be used. Owing…

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Evaluation of the immunoprotective effects of eight recombinant proteins from Baylisascaris schroederi in mice model | Parasites & Vectors

Geng GB. 1864 Wild pandas protection in China makes new achievements–the results of the fourth national panda survey. Green Chin. 2015;422:10–12. Zhang JS, Daszak P, Huang HL, Yang GY, Kilpatrick AM, Zhang S. Parasite threat to panda conservation. Ecohealth. 2008;5:6–9. Article  PubMed  Google Scholar  Qin Z, Liu S, Bai M,…

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Dissociation of Adult DRG for culture or scRNAseq

Reagents and animals Mice or rat 8–12-week-old Media for dissociation Neurobasal A media (Thermo Scientific 10888022) HBSS+H: HBSS (w/o Ca2+ and Mg2+;  and 10% Hepes) Papain (Make Fresh) 45unit Papain (35ul) (Worthington Biochemical LS003126) + 50ul of 20mg/ml L-cysteine (Sigma-Aldrich C7352) + .5ul .5M NaOH + 3ml HBSS+H Put on…

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Whole genome sequencing across clinical trials identifies rare coding variants in GPR68 associated with chemotherapy-induced peripheral neuropathy

Background: Dose-limiting toxicities significantly impact the benefit/risk profile of many drugs. Whole genome sequencing (WGS) in patients receiving drugs with dose-limiting toxicities can identify therapeutic hypotheses to prevent these toxicities. Chemotherapy-induced peripheral neuropathy (CIPN) is a common dose-limiting neurological toxicity of chemotherapies with no effective approach for prevention. Methods: We…

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A CRISPR/Cas9-generated mutation in the zebrafish orthologue of PPP2R3B causes idiopathic scoliosis

Expression of PPP2R3B/ppp2r3b in human foetuses and zebrafish To begin to investigate the requirement for PR70 in vertebrates, we looked for expression of PPP2R3B in human foetuses. The orientation of tissue sections used is given in Supplementary Fig. 1. Using in situ hybridisation, we noted some locations of PPP2R3B expression that…

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NOCICEPTRA2.0 – a comprehensive ncRNA atlas of human native and iPSC-derived sensory neurons

Abstract Non-coding RNAs (ncRNAs) play a critical role in regulating gene expression during development and in the pathogenesis of diseases. In particular, microRNAs have been extensively studied in the context of neurogenesis, the differentiation of pain sensing nociceptive neurons and the pathogenesis of pain disorder, however, little is known about…

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Garden cress seeds ameliorated Aluminum-induced Alzheimers

Introduction Exposure of humans to aluminum has significantly increased worldwide. Potential sources of aluminum exposure include drinking water, food, medicines, vaccines, and aluminum cookware utensils. Aluminum bioaccumulation in the brain has been associated with neuroinflammatory and neurodegenerative changes that might predispose to Alzheimer’s disease (AD).1 Subchronic administration of Al3+ to rats…

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ADC Therapeutics : Annual Report – Form 6-K

ADC Therapeutics SA 2022 Annual Report Table of Contents Letter to Shareholders Business Update Financial Review Corporate Governance Report from the Auditor on the Consolidated IFRS Financial Statements Consolidated IFRS Financial Statements for the Year Ended December 31, 2022 Report from the Auditor on the Statutory Financial Statements of ADC…

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Study shows cutaneous touch neuronal cells to be critical for sexual receptiveness

In a recent study published in Cell, researchers studied the social relevance of G protein-coupled receptor (GPCR) Mrgprb4-lineage touch neurons in sexual touch and receptivity. Study: Touch neurons underlying dopaminergic pleasurable touch and sexual receptivity. Image Credit: Master1305/Shutterstock Background The sensation of a pleasurable touch is initiated by mechanical, electric…

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Overriding Proper Dih. parameters – User discussions

GROMACS version: VERSION 5.1.1GROMACS modification: Yes/NoHere post your questionDear experts,While using ions.tpr command line I am facing errors. Forcefield used: charmm36-mar2019.ffCommand line:gmx grompp -f ions.mdp -c solv.gro -p topol.top -o ions.tpr Ignoring obsolete mdp entry ‘title’ Back Off! I just backed up mdout.mdp to ./#mdout.mdp.2# NOTE 1 [file ions.mdp]:With Verlet…

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Nociceptor neurons affect cancer immunosurveillance

Secondary use of biopsies as research specimens The ten melanoma samples used in this study were collected by Sanford Health and classified by a board-certified pathologist. Their secondary use as research specimens (fully de-identified formalin-fixed, paraffin-embedded (FFPE) blocks) was approved under Sanford Health IRB protocol 640 (titled ‘Understanding and improving…

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Frontiers | Hair Graying Regulators Beyond Hair Follicle

Introduction Hair graying is one of the representative signs of aging. It has been considered to be triggered by a decreased number of follicular melanocyte stem cells (MeSCs; Nishimura et al., 2005; Iida et al., 2020) or dysfunction of MeSCs such as decreased oxidation resistance capacity with aging (Shi et…

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The role of ATXR6 expression in modulating genome stability and transposable element repression in Arabidopsis

Significance The plant-specific H3K27me1 methyltransferases ATXR5 and ATXR6 play integral roles connecting epigenetic silencing with genomic stability. However, how H3K27me1 relates to these processes is poorly understood. In this study, we performed a comprehensive transcriptome analysis of tissue- and ploidy-specific expression in a hypomorphic atxr5/6 mutant and revealed that the…

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[gmx-users] The grompp problem!! (Urgently)

I am doing the Drug-enzyme tutorial.I also generated the drg.itp, drg.gro files using PRODRG server.However, when I use the grompp command before the energy minimizationI face some error A fatal error was shown: atom types CR61 not found The atom type CR61 is presented in the drg.itp file that I…

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r – How to plot a bar plot by ggplot2 and sort bars in non-alphabetical order

This question already has answers here: Closed 11 hours ago. I’m trying to plot a boxplot by ggplot2 and it sorts the boxes in alphabetical order, but I want to change their order. How can I do that? Thanks for any help. Here is my code:…

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iPSCs and DRGs: stepping stones to new pain therapies

doi.org/10.1016/j.molmed.2021.11.005Get rights and content Highlights Voltage-gated sodium (NaV) channels are key drivers of pain in humans and contribute to neuronal excitability and have been regarded as opportune therapeutic targets. The limited success of developing pain therapies targeting NaV channels may reflect the limitations of preclinical screening systems, as heterologous cell…

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KEGG ENZYME: 1.14.12.17

Entry EC 1.14.12.17               Enzyme                                  Name nitric oxide dioxygenase Class Oxidoreductases;Acting on paired donors, with incorporation or reduction of molecular oxygen;With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donorBRITE hierarchy Sysname nitric oxide,NAD(P)H:oxygen oxidoreductase Reaction(IUBMB) 2 nitric oxide + 2 O2 + NAD(P)H =…

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Gene expression profiling of contralateral dorsal root gangl

Introduction Mirror-image pain (MIP) is a mysterious pain phenomenon which is accompanied with many clinical pain conditions.1 MIP develops from the healthy body region which is contralateral to the actual injured site.1–3 MIP is typically characterized by increased mechanical hypersensitivity on the uninjured mirror-image body side.4 It can be triggered…

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