Tag: dsDNA

Phihlikevirus

Phihlikevirus (synonym: PhiH-like viruses) is a genus of viruses in the order Caudovirales, in the family Myoviridae. Bacteria and archaea serve as natural hosts. There is currently only one species in this genus: the type species Halobacterium phage phiH.[1][2] Taxonomy Group: dsDNA [2] Structure Phihlikeviruses are nonenveloped, with a head…

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RNA polymerase II trapped on a molecular treadmill: Structural basis of persistent transcriptional arrest by a minor groove DNA binder

Significance Hairpin pyrrole-imidazole (Py-Im) polyamides can be programmed to bind a broad repertoire of DNA sequences. Py-Im small molecules can be used to target cancer-specific coding regions and block transcription elongation. This transcription blockage by Py-Im cannot be rescued by transcription elongation factors, such as TFIIS. The mechanism by which…

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Anti-dsDNA Testing Specificity for Systemic Lupus Erythematosus: A Systematic Review

Background: Autoantibody specificity in autoimmune diseases is variable due to each patient’s individual spectrum of autoantibodies and the inherent differences between detection methods and tests. Since false-positive results have downstream consequences, we conducted a comprehensive assessment of anti-double stranded DNA (anti-dsDNA) specificity from published studies of systemic lupus erythematosus (SLE)….

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AccuClear Ultra High Sensitivity dsDNA Quantitation Kit with DNA Standard, trial size (200 assays) 31028-T

Description: trial size (200 assays), AccuClear Ultra High Sensitivity dsDNA Quantitation Kit with DNA Standard Category: Nucleic acid quantitation Shipping temperature: 15&deg, C, C to 30&deg Shipping conditions: n/a Storage: 2&deg, C, C to 8&deg Gene target: AccuClear Sensitivity dsDNA Quantitation Kit with DNA trial size (200 assays) Short name:…

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Genus: Ichtadenovirus – Adenoviridae – dsDNA Viruses

Distinguishing features The single known member of this genus, white sturgeon adenovirus 1 (WSAdV-1), is the only confirmed fish adenovirus (Benkő et al., 2002). It was isolated and propagated on a white sturgeon cell line. This host is very divergent from those of other adenoviruses, and phylogenetic calculations and a…

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A viral genome packaging motor transitions between cyclic and helical symmetry to translocate dsDNA

A viral genome packaging motor transitions between cyclic and helical symmetry to translocate dsDNA – Fingerprint — UTMB Health Research Expert Profiles Sort by Weight Alphabetically Medicine & Life Sciences Biopolymers 100% Virus Assembly 98% Computer-Assisted Image Processing 90% Product Packaging 86% Capsid Proteins 85% Viral Genome 85% Proteasome Endopeptidase…

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Low concentrations of a silver-based nanocomposite to manage bacterial spot of tomato in the greenhouse.

Abstract Abstract Bacterial spot, caused by four Xanthomonas spp., is one of the most damaging diseases of tomato worldwide. Due to limited disease management options, growers rely heavily on copper-based bactericides, which are often ineffective due to the presence of copper-resistant Xanthomonas strains. This study was undertaken…

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Efficient target cleavage by Type V Cas12a effectors programmed with split CRISPR RNA

doi: 10.1093/nar/gkab1227. Online ahead of print. Regina Shebanova  1 , Natalia Nikitchina  1   2 , Nikita Shebanov  1 , Vladimir Mekler  3 , Konstantin Kuznedelov  3 , Egor Ulashchik  4 , Ruslan Vasilev  5   6 , Olga Sharko  4 , Vadim Shmanai  4 , Ivan Tarassov  2 , Konstantin Severinov  1   3   7 , Nina Entelis  2…

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Researchers Identify Four Lupus Subgroups Associated with Lupus Outcomes using Long-term Autoantibody Data and Artificial Intelligence

Updated antibody research by Lupus Foundation of America Gary S. Gilkeson Career Development Awardee May Choi identifies subgroups of lupus patients with different outcomes based on long-term autoantibody data with the aid of artificial intelligence. An autoantibody is a type of protein produced when the body’s immune system is attacking…

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Systems biology analysis of human genomes points to key pathways conferring spina bifida risk

Significance Genetic investigations of most structural birth defects, including spina bifida (SB), congenital heart disease, and craniofacial anomalies, have been underpowered for genome-wide association studies because of their rarity, genetic heterogeneity, incomplete penetrance, and environmental influences. Our systems biology strategy to investigate SB predisposition controls for population stratification and avoids…

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Recombinase Polymerase Amplification/Cas12a-Based Identification of Xanthomonas arboricola pv. pruni on Peach

doi: 10.3389/fpls.2021.740177. eCollection 2021. Affiliations Expand Affiliations 1 Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, China. 2 Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan, China. Free PMC article Item in Clipboard Mei Luo et al. Front Plant Sci. 2021. Free PMC article…

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Frontiers | The cGAS-STING Pathway: A Promising Immunotherapy Target

Introduction Invaded by exogenous or endogenous pathogens, the host immune system will be activated accordingly to resist harm and maintain homeostasis, which includes innate immunity and adaptive immunity. As the first line of host immune defense, innate immunity plays a critical role in recognizing extracellular and intracellular pathogens (1, 2)….

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Optimized two-step electroporation process to achieve efficient non-viral-mediated gene insertion into primary T cells

doi: 10.1002/2211-5463.13292. Online ahead of print. Affiliations Expand Affiliations 1 Cellectis Inc, 430E, 29th street, New York, NY, 10016, USA. 2 8 rue de la croix Jarry, 75013, Paris. Item in Clipboard Ming Yang et al. FEBS Open Bio. 2021. Show details Display options Display options Format AbstractPubMedPMID doi: 10.1002/2211-5463.13292. Online…

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US Federal Court Judge Rules BGI Sequencing Chemistries Infringe Illumina Patents

NEW YORK – A Federal Court judge has ruled that BGI’s StandardMPS and CoolMPS products infringe several Illumina patents. In an order issued Thursday, Judge William Orrick of the US District Court for the Northern District of California awarded Illumina’s request for summary judgement on those issues and also rebuffed…

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PacBio sequencing output increased through uniform and directional fivefold concatenation

Strategy and design of the method We sought to develop a simple method to increase the sequencing capability of PacBio CCS to sequence several diverse DNA libraries ~ 870 bp in length that encoded protein variants originating from a directed evolution campaign. To achieve an increase in the throughput of a PacBio sequencing…

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Oncogene Concatenated Enriched Amplicon Nanopore Sequencing for rapid, accurate, and affordable somatic mutation detection | Genome Biology

Stochastic Amplicon Ligation. DNA samples for oncology sequencing are typically extracted from FFPE tissues and can have average lengths of less than 500 nt due to accumulated chemical damage [18]. We developed the Stochastic Amplicon Ligation (SAL) method to enzymatically concatenate many short DNA molecules together to utilize the long-read…

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nanopore sequencing stock

It holds a 15% stake in the company and has valued its holding at £340m, giving a valuation of more than £2bn for the company. . While the company’s current shareholders have recorded its value at just over £2bn, analysts . Essay from the year 2011 in the subject Business…

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Mapping digested synthetic oligos back to original sequences.

Mapping digested synthetic oligos back to original sequences. 0 Hi, I have several synthetic dsDNA of 70bp and I digest them with some enzyme. I am interested to see the exact cut site of the enzyme so I had the products sequenced using MiSeq. They are single-end read. What is…

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The LpoA activator is required to stimulate the peptidoglycan polymerase activity of its cognate cell wall synthase PBP1a

Significance Class A penicillin-binding proteins (aPBPs) assemble the bacterial cell wall and are the targets of penicillin and related β-lactam antibiotics. In gram-negative bacteria, the aPBPs require outer membrane lipoproteins to function. However, little is known about how these proteins promote the activity of their cognate synthases in cells. Here,…

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CRISPR-Based Tech Could Revolutionize Antibody-Based Diagnostics

Lead author, Karl Barber with a PICASSO microarray. [Karl Barber, Schmidt Science Fellows] Scientists have used an adaptation of the genome editing technology CRISPR to develop a new peptide display platform that can be used as a tool to identify antibodies in patient blood samples. Researchers from the Howard Hughes…

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