Tag: EB

Introduction Aminoglycoside antibiotics (AGs) are some of the most commonly prescribed antibacterials, despite their well-known capacity to cause toxic side effects to the kidneys and inner ear (Huth et al., 2011). Although their use in the industrialized world is usually limited to severe infections, they remain very popular in the…

Introduction Currently, the emergence of bacterial resistance poses a significant challenge to public health worldwide. With the widespread use of antimicrobial agents in recent years, the isolation of multidrug-resistant Escherichia coli has increased yearly, particularly among Extended Spectrum β-lactamases (ESBLs) producing strains, which can cause not only multi-site infections but…

Cold Spring Harb Mol Case Stud. 2023 Apr; 9(2): a006256. ,1,2 ,1,2 ,1,2 ,2,3 ,4 and 1,2 Mahvish Q. Rahim 1Pediatric Hematology Oncology, Department of Pediatrics, Indiana University School of Medicine, Indianapolis, Indiana, USA; 2Riley Hospital for Children at Indiana University Health, Indianapolis, Indiana, USA; April Rahrig 1Pediatric Hematology Oncology,…

Flaxman JP, Smith DW, Mackenzie JS, Fraser JRE, Bass SP, Hueston L, et al. A comparison of the diseases caused by Ross River virus and Barmah Forest virus. Med J Aust. 1998;169:159–63. Article  Google Scholar  El-Hage CM, Bamford NJ, Gilkerson JR, Lynch SE. Ross River virus infection of horses: appraisal…

We present a protocol for assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq) specifically on adipocytes using nucleus sorting with adipose tissues isolated from transgenic reporter mice with nuclear fluorescence labeling. ATAC-seq is a powerful technique to understand gene expression regulatory mechanisms. However, adipose tissue is difficult using this technique…

Metagenome-assembled bacterial genomes Out of the 42 ISS metagenomes submitted at NCBI, only PMA-treated metagenomes (n = 21) representing the viable/intact cells were used for generating bacterial MAGs. Characteristics of MAGs (n = 46) such as genome size (2.6 to 6.6 Mb), completeness, contamination percentage, the average mean coverage, number…

If you are interested in reading The Monitor McAllen Texas obituaries, you can find them online or in the print edition of the newspaper. Learn About Online Memorials 269 talking about this. I was honored to have known Jeannie while living and growing up in McAllen. Mrs. Stroud, may you…

Preuss TM. The human brain: rewired and running hot. Ann N Y Acad Sci. 2011;1225 Suppl 1(Suppl 1):E182-191. Article  PubMed  Google Scholar  Roth G, Dicke U. Evolution of the brain and intelligence in primates. Prog Brain Res. 2012;195:413–30. Article  PubMed  Google Scholar  Laland K, Seed A. Understanding human cognitive uniqueness….

Agnes Stephanie Harahap,1– 3 Imam Subekti,4 Sonar Soni Panigoro,5 Asmarinah,6 Lisnawati,1 Retno Asti Werdhani,7 Hasrayati Agustina,8 Dina Khoirunnisa,1 Mutiah Mutmainnah,1 Salinah,1 Alvita Dewi Siswoyo,9 Maria Francisca Ham1,2 1Department of Anatomical Pathology, Faculty of Medicine Universitas Indonesia/Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia; 2Human Cancer Research Center-Indonesian Medical Education and Research Institute,…

Bahram M, Hildebrand F, Forslund SK, Anderson JL, Soudzilovskaia NA, Bodegom PM, et al. Structure and function of the global topsoil microbiome. Nature. 2018;560:233–7. Article  CAS  PubMed  Google Scholar  Waldrop MP, Holloway JM, Smith DB, Goldhaber MB, Drenovsky RE, Scow KM, et al. The interacting roles of climate, soils, and…

Introduction Liver cancer is the third most common cause of cancer death globally with hepatocellular carcinoma (HCC) accounting for the majority of liver cancer.1,2 Early HCC (stage I/II) treatment mainly includes radical surgical resection, radiofrequency ablation and liver transplantation.3,4 However, there is a lack of typical clinical symptoms with early-stage…

The U.S. Food and Drug Administration has approved Vyjuvek, a herpes-simplex virus type 1 (HSV-1) vector-based gene therapy, for the treatment of wounds in patients 6 months of age and older with dystrophic epidermolysis bullosa (DEB) with mutation(s) in the collagen type VII alpha 1 chain (COL7A1) gene.   The approval…

The U.S. Food and Drug Administration has approved Vyjuvek, a herpes-simplex virus type 1 (HSV-1) vector-based gene therapy, for the treatment of wounds in patients 6 months of age and older with dystrophic epidermolysis bullosa (DEB) with mutation(s) in the collagen type VII alpha 1 chain (COL7A1) gene.   The approval…

Challenges & Requirements [SPONSORED GUEST ARTICLE]   International Supercomputing Conference (ISC) is an annual conference that showcases the key technologies in the supercomputing field. It brings together experts from multiple specialist fields, including research into life sciences and genetics to shed light on the origins and mysteries in our lives. HPC,…

Bhatt S, Gething PW, Brady OJ, Messina JP, Farlow AW, Moyes CL, et al. The global distribution and burden of dengue. Nature. 2013;496:504–7. Article  CAS  PubMed  PubMed Central  Google Scholar  Stanaway JD, Shepard DS, Undurraga EA, Halasa YA, Coffeng LE, Brady OJ, et al. The global burden of dengue: an…

Researchers at Stanford have developed a targeted gene therapy approach to treat patients with recessive dystrophic epidermolysis bullosa (RDEB). Epidermolysis bullosa (EB) represents a group of debilitating inherited skin disorders in which blisters develop after minor trauma to the skin. Symptoms and severity depend on type of EB. RDEB is…

Tanzi RE, Bertram L. New frontiers in Alzheimer’s disease genetics. Neuron. 2001;32:181–4. Article  CAS  PubMed  Google Scholar  Holland D, Frei O, Desikan R, Fan C-C, Shadrin AA, Smeland OB, et al. The genetic architecture of human complex phenotypes is modulated by linkage disequilibrium and heterozygosity. Genetics. 2021;217:iyaa046. Article  PubMed  PubMed…

Bergmann, O. et al. Evidence for cardiomyocyte renewal in humans. Science 324, 98–102 (2009). Article  CAS  PubMed  PubMed Central  Google Scholar  Senyo, S. E. et al. Mammalian heart renewal by pre-existing cardiomyocytes. Nature 493, 433–436 (2013). Article  CAS  PubMed  Google Scholar  Bergmann, O. et al. Dynamics of cell generation and…

CLEVELAND, May 16, 2023 (GLOBE NEWSWIRE) — Abeona Therapeutics Inc. ( ABEO) today announced new data regarding three internally developed preclinical gene therapy product candidates from its adeno-associated virus (AAV) ophthalmology program that will be presented at the 26th Annual Meeting of the American Society of Gene & Cell Therapy…

Abeona Therapeutics Inc. Animal proof-of-concept for AAV-based gene therapies for Stargardt disease, X-linked retinoschisis and autosomal dominant optic atrophy CLEVELAND, May 16, 2023 (GLOBE NEWSWIRE) — Abeona Therapeutics Inc. (Nasdaq: ABEO) today announced new data regarding three internally developed preclinical gene therapy product candidates from its adeno-associated virus (AAV) ophthalmology…

Baumgart DC, Sandborn WJ. Crohn’s disease. Lancet. 2012;380(9853):1590–605. Article  PubMed  Google Scholar  Bernstein CN. Treatment of IBD: where we are and where we are going. Am J Gastroenterol. 2015;110(1):114–26. Article  PubMed  Google Scholar  Sies H. Oxidative stress: a concept in redox biology and medicine. Redox Biol. 2015;4:180–3. Article  CAS  PubMed …

Screening for genetic defects in the cells should be examined for clinical application. The Pearson syndrome (PS) patient harbored nuclear mutations in the POLG and SSBP1 genes, which could induce systemic large-scale mitochondrial genome (mtDNA) deletion. We investigated iPSCs with mtDNA deletions in PS patient and whether deletion levels could…

Sample selection We identified parent–child trios from the 1KG in which the child cell line banked within the NHGRI Sample Repository for Human Genetic Research at the Coriell Institute for Medical Research was listed as having zero expansions and two or fewer passages, and rank-ordered representative individuals as follows. Loci…

Tapper EB, Curry MP. Hepatitis Caused by Other Viruses Handbook of Liver Disease, 2018: p. 78. Sarin SK, Kumar M. Viral hepatitis A, in Molecular Pathology of Liver Diseases. Springer; 2011. pp. 527–52. Qu B, Brown RJ. Strategies to inhibit Hepatitis B Virus at the transcript level. Viruses. 2021;13(7):1327. Article  CAS …

scEC&T sequencing A detailed, step-by-step protocol of scEC&T-seq is available on the Nature Protocol Exchange46 and is described below. The duration of the protocol is approximately 8 days per 96-well plate. Cell culture Human tumor cell lines were obtained from ATCC (CHP-212) or were provided by J. J. Molenaar (TR14; Princess…

Alkooranee JT, Liu S, Aledan TR, Yin Y, Li M (2015) First report of powdery mildew caused by erysiphe cruciferarum on brassica napus in China. Plant Dis 99(11):1651 CrossRef  Google Scholar  Al-Lami HFD, You MP, Barbetti MJ (2019a) Incidence, pathogenicity and diversity of Alternaria spp. associated with alternaria leaf spot…

Review . 2023 Apr 24;14(4):160-170. doi: 10.5306/wjco.v14.i4.160. Affiliations Expand Affiliations 1 Department of Hematology, Hospital Universitario de Gran Canaria Dr. Negrín, Las Palmas 35019, Spain. 2 Department of Anatomical Pathology, Hospital Universitario de Gran Canaria Dr. Negrín, Las Palmas 35019, Spain. 3 Department of Hematology, Hospital Universitario de Gran Canaria…

Liang Y, Lin H, Zou L, Deng X, Tang S. Biosens Bioelectron. 2022;205:114098. Article  CAS  PubMed  PubMed Central  Google Scholar  Telenti A, Hodcroft EB, Robertson DL. Cold Spring Harb Perspect Med. 2022;12:a041390. Article  CAS  PubMed  Google Scholar  Shrestha LB, Foster C, Rawlinson W, Tedla N, Bull RA. Reviews in Medical…

Plant material Streptocarpus grandis (RBGE lineage 19771210) and Streptocarpus kentaniensis (RBGE lineage 19951992) were cultivated in the glasshouses at the Royal Botanic Garden Edinburgh. DNA extraction method for Streptocarpus PacBio HiFi long-read sequencing To develop the protocol, small scale extractions were carried out with approx. 3 g leaf tissue as input…

Hi, I think I might have found a bug in featureCounts from Rsubread (v2.12.3). I am trying to find reads overlapping exon junctions from a personalised reference, using Nanopore long read BAMs. I am afraid I cannot share fully reproducible code as I am using my own reference, but this…

ximushushu/iStock via Getty Images Abeona Therapeutics (NASDAQ:ABEO) is a good speculative biotech play to look into. The reason why I state that is because it is gearing up to file a Biologics Licensing Application [BLA] to the FDA of EB-101 for the treatment of patients with recessive dystrophic epidermolysis bullosa…

By Wayne Robinson | 26 April 2023 NSW print and wrap specialist Killer Image has taken out the Ball & Dogget MetaWrap – Are You Australia’s Greatest Wrapper competition, for its Coopers Camo Truck job. Winner: Killer Images’ truck job After receiving an overwhelming number of submissions from across the…

 After an ‘overwhelming’ number of entries, the winner of Ball & Doggett’s MetaWrap – Are YOU Australia’s Greatest Wrapper? competition is Coopers Camo Truck by Killer Image from Glendenning in Sydney, NSW.    “After receiving an overwhelming number of submissions from across the country, judges – Matt Horne (Wrap Studio),…

Lom J, Dyková I. Myxozoan genera: definition and notes on taxonomy, life-cycle terminology and pathogenic species. Folia Parasitol. 2006;53:1–36. Article  Google Scholar  Køie M. Parvicapsula spinachiae n. sp. (Myxozoa, Parvicapsulidae) in Spinachia spinachia (L.) (Teleostei, Gasterosteidae) from Denmark. Parasitol Res. 2003;90:445–8. Article  PubMed  Google Scholar  Jones S, Prosperi-Porta G, Dawe…

Cell lines and tissue culture The murine 17-CL1 cell line (derived from 3T3 cells, BEI Resources, Cat. No. NR-53719) was maintained in Dulbecco Modified Eagle Medium (DMEM, Life Technologies) supplemented with 10% Fetal Bovine Serum (FBS, Gemini Bio-Products), L-glutamine (Invitrogen), and Sodium pyruvate (Invitrogen). The NCTC clone 1469 cell line…

The global genomics market is anticipated to grow at a considerable CAGR of 15.3% during the forecast period. The key factors that are contributing to the market growth are key players operating in the market are adopting various strategies such as acquisition, mergers, new product launches, and other are such…

Martiny JBH, Bohannan BJM, Brown JH, Colwell RK, Fuhrman JA, Green JL, et al. Microbial biogeography: putting microorganisms on the map. Nat Rev Microbiol. 2006;4:102–12. Article  CAS  PubMed  Google Scholar  Hanson CA, Fuhrman JA, Horner-Devine MC, Martiny JBH. Beyond biogeographic patterns: processes shaping the microbial landscape. Nat Rev Microbiol. 2012;10:497–506….

Genome-scale CRISPR screen to identify regulators that maintain mESC pluripotency To establish a function-based PGRN, we first performed a CRISPR-Cas9 mediated genome-wide screen to detect genes essential for self-renewal. mESCs were cultured under Leukaemia inhibitory factors (LIF)/serum condition (L/S), which was commonly used in similar tasks and confer a naïve…

The TRACERx 421 cohort The TRACERx study (clinicaltrials.gov/ct2/show/NCT01888601) is a prospective observational cohort study that aims to transform our understanding of NSCLC, the design of which has been approved by an independent research ethics committee (13/LO/1546). Informed consent for entry into the TRACERx study was mandatory and obtained from every…

Bisoffi Z, Buonfrate D, Montresor A, Requena-Méndez A, Muñoz J, Krolewiecki AJ, et al. Strongyloides stercoralis: a plea for action. PLoS Negl Trop Dis. 2013;7:5. Google Scholar  Schär F, Trostdorf U, Giardina F, Khieu V, Muth S, Marti H, et al. Strongyloides stercoralis: global distribution and risk factors. PLoS Negl…

doi: 10.1186/s13059-023-02911-2. Affiliations Expand Affiliations 1 Institute of Human Genetics, UMR9002, CNRS and Université de Montpellier, Montpellier, France. 2 DIADE, University of Montpellier, CIRAD, IRD, Montpellier, France. 3 IFB – Southgreen Bioversity, CIRAD, INRAE, IRD, Montpellier, France. 4 TAGC, UMR 1090 INSERM, Marseille, France. 5 ISEM, Université Montpellier, CNRS, IRD,…

Almazán C, González-Álvarez VH, Fernández de Mera IG, Cabezas-Cruz A, Rodríguez-Martínez R, De la Fuente J (2016) Molecular identification and characterization of Anaplasma platys and Ehrlichia canis in dogs in Mexico. Ticks Tick Borne Dis 7:276–283. doi.org/10.1016/j.ttbdis.2015.11.002 Article  PubMed  Google Scholar  Aman R, Mahas A, Marsic T, Hassan N, Mahfouz…

The ability to alter the genetic composition of living cells has revolutionized biology. Scientific advances, from treating genetic disorders through gene therapy to reprograming skin cells into neurons, have been made possible by the increasing proficiency in introducing foreign nucleic acids (DNA and RNA) into cells using a technique known…

Introduction Infections are the major cause of human deaths worldwide and present a rising threat to public security.1 Antimicrobial resistance is a major concern that is threatening our ability to treat bacterial infections.2 Delayed administration of antibiotics significantly increases the in-hospital mortality rate.3 The tragic outcome of infection highlights the…

Naik RR, Singamaneni S. Introduction: bioinspired and biomimetic materials. Chem Rev. 2017;117(20):12581–3. Article  CAS  PubMed  Google Scholar  Huang G, Li F, Zhao X, Ma Y, Li Y, Lin M, et al. Functional and biomimetic materials for engineering of the three-dimensional cell microenvironment. Chem Rev. 2017;117(20):12764–850. Article  CAS  PubMed  PubMed Central …

cacheebeyesearch(1) Generates server cache file for EB-eye search domains SYNOPSIS cacheebeyesearch [-outfile outfile] -cachefile outfile cacheebeyesearch -help DESCRIPTION cacheebeyesearch is a command line program from EMBOSS (“the European Molecular Biology Open Software Suite”). It is part of the “Data resources” command group(s). OPTIONS Input section Output section -outfile outfile Default value: stdout…

In LAMMPS I am depositing H on Si surface. I want to calculate the dipole moment of the bonds created after deposition. However, I can see that LAMMPS calculate the overall dipole moments of Si and H chunks. Is there any way to see Dipole Moment of a specific bond?…

NEW YORK – Abeona Therapeutics Inc. (Nasdaq: ABEO) today announced that additional data from its pivotal Phase 3 VIITAL study of EB-101 for recessive dystrophic epidermolysis bullosa (RDEB) has been accepted for an oral presentation at the inaugural International Societies for Investigative Dermatology (ISID) Meeting, being held May 10-13, 2023…

I have used Minimap2 to create a paf file by aligning a Fastq file against itself. Now from this Paf file I can see where the reads overlap, and I want to take these positions, and use them to extract the entire sequences between these positions from the paf file…

Bredesen DE, Rao RV, Mehlen P. Cell death in the nervous system. Nature. 2006;443:796–802. Article  CAS  PubMed  PubMed Central  Google Scholar  Fricker M, Tolkovsky AM, Borutaite V, Coleman M, Brown GC. Neuronal cell death. Physiol Rev. 2018;98:813–80. Article  CAS  PubMed  PubMed Central  Google Scholar  West MJ, Coleman PD, Flood DG,…

These authors contributed equally: Marine Armand, Michaël Degaud. These authors jointly supervised this work: Olivier Hermine, Frédéric Davi, Lucile Couronné. Hematology Laboratory, Hôpital Pitié-Salpêtrière, Assistance-Publique-Hôpitaux de Paris and Sorbonne University, Paris, France Marine Armand, Michaël Degaud & Frédéric Davi Centre de Recherche des Cordeliers, Sorbonne Université, Université Paris Cité, Inserm, Cell Death and…

Introduction Chronic obstructive pulmonary disease (COPD) is a common chronic respiratory condition that mostly occurs in middle-aged and elderly people. COPD manifests as a progressive airflow limitation associated with an abnormal lung inflammatory response.1 As a disease with high incidence, disability rate, long disease course, and multiple comorbidities, COPD has…

Here is the database with 3,898 ncRNA that was compared to the set of lncRNA indentified by us. The database was built based on the papers below: Berretta J, Pinskaya M, Morillon A (2008) A cryptic unstable transcript mediates transcriptional trans -silencing of the Ty1 retrotransposon in S. cerevisiae. Genes…

Stairs CW, Leger MM, Roger AJ. Diversity and origins of anaerobic metabolism in mitochondria and related organelles. Philos Trans R Soc B: Biol Sci. 2015;370:20140326. Article  Google Scholar  Eme L, Spang A, Lombard J, Stairs CW, Ettema TJG. Archaea and the origin of eukaryotes. Nat Rev Microbiol. 2017;15:711–23. Article  CAS …

Miles JH. Autism spectrum disorders-a genetics review. Genet Med. 2011;13:278–94. Article  PubMed  Google Scholar  Betancur C. Etiological heterogeneity in autism spectrum disorders: more than 100 genetic and genomic disorders and still counting. Brain Res. 2011;1380:42–77. Article  CAS  PubMed  Google Scholar  Satterstrom FK, Kosmicki JA, Wang J, Breen MS, De Rubeis…

03 Mar 2023 Of the dozens of loci genome-wide association studies have linked to Alzheimer’s, most are mysterious to scientists. How do they contribute to disease? Much work focuses on changes in expression of individual genes, but as reported in a preprint posted to medRxiv on February 8, scientists led…

QIAseq FX DNA Library Kit Protocol Procedure: 1. Acquire high quality gDNAsamples. 2. Set up a thermocycler to incubate for 30 minutes at 32℃, with the heated lid at 70℃. Run the program and pause until the block reaches 4℃. 3. In a tube on ice, prepare and mix the…

Introduction Klebsiella pneumoniae (KPN) is a common gram-negative bacterium that causes clinical infection, and studies have shown that it has the second-highest detection rate among all bacterial infections.1,2 Compared with common KPN (ie classic KPN [cKP]), hypervirulent KPN (HvKP) has the following characteristics: (1) Usually, HvKP colonies display a high-mucus…

Alizadeh AA, Eisen MB, Davis RE, Ma C, Lossos IS, Rosenwald A, Boldrick JC, Sabet H, Tran T, Yu X, Powell JI, Yang L, Marti GE, Moore T, Hudson J, Lu L, Lewis DB, Tibshirani R, Sherlock G, Chan WC, Greiner TC, Weisenburger DD, Armitage JO, Warnke R, Levy R,…

By Alden Graham · Posted 1 hour ago Please help. Minecraft is crashing and not responding because of error. Code: —- Minecraft Crash Report —- // Surprise! Haha. Well, this is awkward. Time: 2023-02-20 19:29:33 Description: Initializing game java.lang.RuntimeException: null at net.minecraftforge.registries.GameData.postRegisterEvents(GameData.java:326) ~[forge-1.19.2-43.2.4-universal.jar%23318!/:?] {re:mixin,re:classloading,pl:mixin:APP:supermartijn642corelib.mixins.json:GameDataMixin,pl:mixin:A} at net.minecraftforge.common.ForgeStatesProvider.lambda$new$4(ForgeStatesProvider.java:25) ~[forge-1.19.2-43.2.4-universal.jar%23318!/:?] {re:classloading} at net.minecraftforge.fml.ModLoader.lambda$dispatchAndHandleError$23(ModLoader.java:207)…

Data Bridge Market Research analyzes that the Asia-Pacific plant breeding and CRISPR plant market is expected to reach the value of USD 6.194.66 million by 2029, at a CAGR of 18.1% during the forecast period. Type accounts for the largest type segment in the market due to rapid demand of…

How to decide the best threshold for filtering probes in limma 0 I am using an approach where I fit the model using all the probe sets and then subsequently at the eBayes step, set a filter and then on the eBayes object use “plotSA” to see if there is…

The global biochips market is growing at a CAGR of nearly 13.5% during the forecast period. The market growth is mainly attributed to the rising technological innovations in biochips. The rising innovations in biochips have been gradually rising over the years to obtain high throughput in less time. Therefore, the…

Ou A, Yung WKA, Majd N. Molecular mechanisms of treatment resistance in glioblastoma. Int J Mol Sci. 2021;22(1):351. CAS  Google Scholar  Wang G-M, Cioffi G, Patil N, Waite KA, Lanese R, Ostrom QT, et al. Importance of the intersection of age and sex to understand variation in incidence and survival…

Vos T, Barber RM, Bell B, Bertozzi-Villa A, Biryukov S, Bolliger I, et al. Global, regional, and national incidence, prevalence, and years lived with disability for 301 acute and chronic diseases and injuries in 188 countries, 1990-2013: a systematic analysis for the Global Burden of Disease Study 2013. Lancet. 2015;386:743–800….

Lyon GR. Part I defining dyslexia, comorbidity, teachers’ knowledge of language and reading. Ann Dyslexia. 2003;53:1–14. Google Scholar  Hendren RL, Haft SL, Black JM, White NC, Hoeft F. Recognizing psychiatric comorbidity with reading disorders. Front Psychiatry 2018;9:101. Google Scholar  Doust C, Fontanillas P, Eising E, Gordon SD, Wang Z, Alagoz…

Log in to MyKarger to check if you already have access to this content. Buy FullText & PDF Unlimited re-access via MyKarger Unrestricted printing, no saving restrictions for personal use read more CHF 38.00 * EUR 35.00 * USD 39.00 * …

Nazneen January 29, 2023, 7:24pm #1 Hi.I want a system to run under certain temperature (50~70 Kelvin). I used following commands for velocity and temperature: velocity hydrogen create 70 7867431 rot yes dist gaussianfix 1 all nvt temp 70 70 100 tchain 1thermo_style custom step pe lx ly lz press…

Belliveau J, Papoutsakis ET (2022) Extracellular vesicles facilitate large-scale dynamic exchange of proteins and RNA among cultured Chinese hamster ovary and human cells. Biotechnol Bioeng 119(5):1222–1238. doi.org/10.1002/bit.28053 Article  CAS  Google Scholar  Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle…

Cell culture and treatments Bladder cancer cells lines RT4, MGHU3, RT112, KU19-19, JMSU1, T24 and TCCSup were grown in RPMI-1640 medium (Life Technologies, Carlsbad, CA, USA), supplemented with 10% Fetal Bovine Serum (FBS; Eurobio, Courtaboeuf, France). Normal human urothelium (NHU) cells were from Jennifer Southgate (University of York, UK). NHU were…

Rar V, Tkachev S, Tikunova N. Genetic diversity of Anaplasma bacteria: twenty years later. Infect Genet Evol. 2021;91:104833. Google Scholar  Atif FA. Alpha proteobacteria of genus Anaplasma (Rickettsiales: Anaplasmataceae): epidemiology and characteristics of Anaplasma species related to veterinary and public health importance. Parasitology. 2016;143:659–85. Google Scholar  Battilani M, de Arcangeli…

Benelli G, Wilke A, Beier JC. Aedes albopictus (Asian Tiger Mosquito). Trends Parasitol. 2020;36:942–3. Article  Google Scholar  Bhatt S, Gething PW, Brady OJ, Messina JP, Farlow AW, Moyes CL, et al. The global distribution and burden of dengue. Nature. 2013;496:504–7. Article  CAS  Google Scholar  Bonizzoni M, Gasperi G, Chen X,…

dc.description.abstract © 2020, The Author(s). The availability of high-quality RNA-sequencing and genotyping data of post-mortem brain collections from consortia such as CommonMind Consortium (CMC) and the Accelerating Medicines Partnership for Alzheimer’s Disease (AMP-AD) Consortium enable the generation of a large-scale brain cis-eQTL meta-analysis. Here we generate cerebral cortical eQTL from…

Ai H, Fang X, Yang B, Huang Z, Chen H, Mao L, Zhang F, Zhang L, Cui L, He W, Yang J, Yao X, Zhou L, Han L, Li J, Sun S, Xie X, Lai B, Su Y, Lu Y, Yang H, Huang T, Deng W, Nielsen R, Ren J,…

2020 Alzheimer’s disease facts and figures. Alzheimers Dement 2020. Mayeda ER, Glymour MM, Quesenberry CP, Whitmer RA. Inequalities in dementia incidence between six racial and ethnic groups over 14 years. Alzheimers Dement. 2016;12:216–24. Article  Google Scholar  Matthews KA, Xu W, Gaglioti AH, Holt JB, Croft JB, Mack D, et al….

Electroporation Instruments Market The Global Electroporation Instruments Market is estimated to attain a valuation of US$ 244 Mn by the end of 2030, states a study by Transparency Market Research (TMR). Besides, the report notes that the market is prognosticated to expand at a CAGR of 6% during the forecast period,…

Synthetic Biology Market Outlook 2031 The global synthetic biology market was valued at US$ 10.7 Bn in 2021 The global market is projected to advance at a CAGR of 21.3% from 2022 to 2031 The global synthetic biology market is anticipated to reach more than US$ 74.7 Bn by the end of 2031 Synthetic Biology Market Introduction Synthetic biology is a branch of science…

Forensic Technologies Market: Introduction According to the report, the global forensic technologies market was valued at US$ 51.6 Bn in 2020 and is projected to expand at a CAGR of 13.2% from 2021 to 2028. The forensic technology refers to the scientific system of searching, investigating, retrieving, and analyzing the evidence gathered…

Bioinformatics Market: Introduction According to the report, the global bioinformatics market was valued at US$ 24.7 Bn in 2020 and is projected to expand at a CAGR of 5.9% from 2021 to 2028. Bioinformatics is used to manage the data gathered from research & development projects in the biopharmaceutical, life sciences, and biotechnology industries. Growth of the…

Wilmington, Delaware, United States, Transparency Market Research Inc. – The global injectable drugs market is relied upon to enlist a strong double-digit CAGR within the forecast period from 2019 to 2027. The market is majorly classified on the basis of drug-class, geography, molecule-type, and application. From the previous one entire…

Wilmington, Delaware, United States, Transparency Market Research Inc. – The global healthcare additive manufacturing market is growing due to the rising demand for personalized medical devices, such as implants, and the advent of sophisticated technology to manufacture a variety of products with complex and simple designs. Additive manufacturing is widely…

Wilmington, Delaware, United States, Transparency Market Research Inc.: Agrigenomics is defined as the study of the genetic makeup of crops and livestock and how genes influence the produce. Application of genomics in agriculture enables improvement in the sustainability and productivity in livestock and crop production. Read Report Overview – www.transparencymarketresearch.com/agrigenomics-market.html…

Wilmington, Delaware, United States: The demand in the global bioimpendance analysers market is surging owing to high growth potential amongst emerging economies such as Brazil and India. Rising private as well as public initiatives and investments aimed at supporting medical and healthcare research as well as increase in the domestic…

Global Restriction Endonucleases Products (REP) Market: Overview Albany NY, United States: The leading applications for the products and solutions offered by the manufacturers and players functional in the landscape of the global restriction endonucleases products market include epigenetics, fragment length polymorphism or RFLP, cloning, sequencing, polymerase chain reaction, and restriction…

We present detailed protocols for the generation and characterization of 2D and 3D human induced pluripotent stem cell (hIPSC)-based models of neocortical development as well as complementary methodologies enabling qualitative and quantitative analysis of primary cilium (PC) biogenesis and function. The overall goal of the procedure we detail here is…

1. Sharma VK. Adaptive significance of circadian clocks. Chronobiol Int. 2003;20(6):901–19. PubMed  Google Scholar  2. Paranjpe DA, Sharma VK. Evolution of temporal order in living organisms. J Circadian Rhythms. 2005;3(1):7. PubMed  PubMed Central  Google Scholar  3. Yerushalmi S, Green RM. Evidence for the adaptive significance of circadian rhythms. Ecol Lett….

Trimmomatic parameters 0 $java -jar /apps/eb/Trimmomatic/0.39-Java-1.8.0_144/trimmomatic-0.39.jar PE -phred33 seq1_L2_1.fq.gz seq1_L2_2.fq.gz _L2_r1_paired_fq.gz seq1_L2_r1_unpaired.fq.gz seq_L2_r2_paired.fq.gz Seq1_L2_r2_unpaired.fq.gz ILLUMINACLIP:TruSeq3-PE.fa:2:30:10 LEADING:3 TRAILING:3 SLIDINGWINDOW:4:15 MINLEN:5 ILLUMINACLIP:TruSeq3-PE.fa:2:30:10 LEADING:3 TRAILING:3 SLIDINGWINDOW:4:15 MINLEN:5 Trimmomatic • 137 views • link updated 15 hours ago by GenoMax 110k • written 17 hours ago by ronny • 0 Login before adding your…

Embryonic stem cells (ESCs) are pluripotent stem cells that have indefinite self-renewal capacities under appropriate culture conditions in vitro. The pluripotency maintenance and proliferation of these cells are delicately governed by the concert effect of a complex transcriptional regulatory network. Herein, we discovered that p57Kip2 (p57), a cyclin-dependent kinase inhibitor…

1. Castle JT. Cholesteatoma pearls: practical points and update. Head Neck Pathol. 2018;12(3):419–29. Article  Google Scholar  2. Xie S, Wang X, Ren J, Liu W. The role of bone resorption in the etiopathogenesis of acquired middle ear cholesteatoma. Eur Arch Otorhinolaryngol. 2017;274(5):2071–8. Article  Google Scholar  3. Bhutta MF, Williamson IG,…

1. Szathmáry E, Smith JM. The major evolutionary transitions. Nature 1995;374:227–32. PubMed  Google Scholar  2. West SA, Fisher RM, Gardner A, Kiers ET. Major evolutionary transitions in individuality. Proc Natl Acad Sci USA. 2015;112:10112–9. CAS  PubMed  PubMed Central  Google Scholar  3. Moran NA. The coevolution of bacterial endosymbionts and phloem-feeding…

A healthy soil nourishes plants and animals, purifies water and air, and promotes sustainable agriculture. Characteristic for highly complex and competitive soil ecosystems are the frequent and direct interactions between all soil-dwelling microorganisms, animals, and plants (1, 2), all of which need to be provided with minerals and carbon sources….

Introduction Mirror-image pain (MIP) is a mysterious pain phenomenon which is accompanied with many clinical pain conditions.1 MIP develops from the healthy body region which is contralateral to the actual injured site.1–3 MIP is typically characterized by increased mechanical hypersensitivity on the uninjured mirror-image body side.4 It can be triggered…

Witawat Tunyong,1 Weewan Arsheewa,2 Sirijan Santajit,3,4 Thida Kong-ngoen,1 Pornpan Pumirat,1 Nitat Sookrung,5,6 Wanpen Chaicumpa,6 Nitaya Indrawattana1 1Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand; 2Department of Microbiology, Phrapokklao Hospital, Chanthaburi, 22000, Thailand; 3School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat, 80161, Thailand;…