Tag: electrotransfection
Optimization of piggyBac Transposon System Electrotransfection in Sheep Fibroblasts
doi: 10.1007/s12033-023-00659-5. Online ahead of print. Affiliations Expand Affiliations 1 College of Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, Shanxi, China. 2 School of Life Science and Engineering, Foshan University, Foshan, 528000, Guangdong, China. 3 College of Life Science, Shanxi Agricultural University, Taigu, 030801, Shanxi, China. 4 Center of Experiment…
Optimization of conditions for intrauterine electroporation of neural stem cells in the subependymal region of fetal rat brains
Explanation of the title: Neural precursor cells: Multipotent cells capable of self-proliferation and differentiation, which can maintain the desired number of neural precursor cells through proliferation, and can also differentiate into neurons, astrocytes and oligodendrocytes. In utero electrotransfection technique: In utero electroporation is an important technique to study the molecular…
An electrochemical protocol for CRISPR-mediated gene-editing of sheep embryonic fibroblast cells
Genetic engineering of farm animals is commonly carried out via cell-mediated transfection followed by somatic cell nuclear transfer. However, efficient transfer of exogenous DNA into ovine embryonic fibroblast (EF) cells without compromising cell viability have remained a challenging issue. Here, we aimed to develop a protocol for electrotransfection of sheep…
Eyevensys Enters Collaboration with Phillips-Medisize and Minnetronix Medical
Get inside Wall Street with StreetInsider Premium. Claim your 1-week free trial here. Manufacturing of Eyevensys’ core ocular device and electrical pulse generator technology covered under a new collaboration PARIS & CAMBRIDGE, Mass.–(BUSINESS WIRE)– Eyevensys, a privately held, clinical-stage biotechnology company developing non-viral gene therapies for ophthalmic diseases, today announces…
