Tag: ensembl

Transcriptional reprogramming of skeletal muscle stem cells by the niche environment

Isolation of pure populations of MuSCs and niche cells by fluorescence-activated cell sorting (FACS) Hindlimb muscles were dissected from young (4–6 weeks old) or aged (22–26 months old) C57BL/6J (Jackson Laboratory, 000664) mice and minced until no visible tissue chunks were visible. Muscle was then digested in a 15 ml Falcon…

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Bioinformatics Analyst I Job in Maryland (MD), Other Career, Full Time Jobs in Frederick National Laboratory for Cancer Research

Bioinformatics Analyst I Job ID: req3323Employee Type: exempt full-timeDivision: Cancer Research Technology ProgramFacility: Frederick: ATRFLocation: 8560 Progress Dr, Frederick, MD 21701 USA The Frederick National Laboratory is a Federally Funded Research and Development Center (FFRDC) sponsored by the National Cancer Institute (NCI) and operated by Leidos Biomedical Research, Inc….

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How To Install r-bioc-annotationhub on Kali Linux

In this tutorial we learn how to install r-bioc-annotationhub on Kali Linux. r-bioc-annotationhub is GNU R client to access AnnotationHub resources Introduction In this tutorial we learn how to install r-bioc-annotationhub on Kali Linux. What is r-bioc-annotationhub This package provides a client for the Bioconductor AnnotationHub web resource. The AnnotationHub…

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Bioinformatics Research Scientist Job in Pennsylvania

UPCI Basic Research Administration – Pennsylvania-Pittsburgh – (23000703) A Bioinformatics Research Scientist position is available in the laboratory of Dr. Yu-Chiao Chiu at the UPMC Hillman Cancer Center at the University of Pittsburgh School of Medicine. We are seeking a highly motivated candidate with strong computational skills in…

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Annotating snps with gene information

Annotating snps with gene information 0 Hi, I am trying to annotate a list of snps with the ENSG gene number using biomaRt. I need to use ensemble version 91. I have built the following query: snps = c(“rs201327123” “rs141149254” “rs114420996” “rs62637817″) ensembl.snp = useEnsembl(biomart=”snps”, version=91) mart.snp <- useMart(biomart =…

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a problem with getBM

I want to add gene name information to my expression file, I use Biomart for this, but actually i did not get any result from getBM, my codes in R: upregul<-read.csv(file.choose()) View(upregul) library(biomaRt) listEnsembl() ensembl <- useEnsembl(biomart = “genes”) ensembl_ids<-(upregul) View(ensembl_ids) datasets <- listDatasets(ensembl) head(datasets) ensembl_con<-useEnsembl(“ensembl”,dataset=”hsapiens_gene_ensembl” ) attr<-listAttributes(ensembl_con) filters<-listFilters(ensembl_con) theBm<-getBM(attributes…

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Remote Visualization of Local Genome Alignments Aids Pathogenic Variant Evaluation for Rare Disease

CHICAGO – A group at Spain’s National Center for Genomic Analysis-Center for Genomic Regulation (CNAG-CRG) in Barcelona has harnessed a protocol for accessing sequencing and variant data to help assess potentially pathogenic genetic variants within the context of a European Union-funded program to improve diagnosis of rare diseases. The CNAG-CRG…

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tximeta accessing gene symbols after addIds()

Hi, I’m sure I’m missing something obvious, but is there an easy way to access the gene symbols after adding them with the addIds() function in tximeta? gse <- addIds(gse, “SYMBOL”, gene=TRUE) mcols(gse) DataFrame with 60240 rows and 3 columns gene_id <character> ENSG00000000003.15 ENSG00000000003.15 ENSG00000000005.6 ENSG00000000005.6 ENSG00000000419.12 ENSG00000000419.12 ENSG00000000457.14 ENSG00000000457.14…

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What should I do if the gene IDs in my reference dataset and test dataset are different

What should I do if the gene IDs in my reference dataset and test dataset are different 1 Hi everyone, I am trying to annotate my single cell dataset (integrated dataset) using singleR. However, I am getting the error “no common genes between ‘test’ and ‘ref’”. I realized that the…

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Exon number between GTF file and ENSEMBL site does not match

Based on the ENSEMBL GTF file, I counted the number of exons per gene as follows: awk ‘$3 == “exon” {print}’ Homo_sapiens.GRCh38.108.gtf | gffread -F –keep-exon-attrs –table “gene_id”,”transcript_id”,@numexons | sort -k1,1 | datamash -sg1 max 3 > gene_lists/exons_per_gene.txt An extract of the output is: ENSG00000034677 11 ENSG00000034693 12 ENSG00000034713 4…

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get build 37 positions from dbSNP rsIDs

get build 37 positions from dbSNP rsIDs 4 $ mysql –user=genome –host=genome-mysql.cse.ucsc.edu -A -D hg19 -e ‘select chrom,chromStart,chromEnd,name from snp147 where name in (“rs371194064″,”rs779258992″,”rs26″,”rs25”)’ +——-+————+———-+————-+ | chrom | chromStart | chromEnd | name | +——-+————+———-+————-+ | chr7 | 11584141 | 11584142 | rs25 | | chr7 | 11583470 | 11583471…

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Ensembl ID to Gene Symbol Converter

Ensembl ID to Gene Symbol Converter 0 I’m trying to convert ensembl gene IDs to gene symbols. The ensembl gene IDs come from human reference file: Homo_sapiens.GRCh38.dna.primary_assembly.fa. I’ve tried using the BioMart tool from ensembl and the BioTools Converter (www.biotools.fr/human/ensembl_symbol_converter), but both tools still give me a lot of blank…

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Junior Bioinformatics Analyst Job in Maryland (MD), Bioinformatics Career, Full Time Jobs in Frederick National Laboratory for Cancer Research

Junior Bioinformatics Analyst Job ID: req3312Employee Type: exempt full-timeDivision: Bioinformatics and Computational ScienceFacility: NIHLocation: NIH Campus 9000 Rockville Pike, Bethesda, MD 20892 USA The Frederick National Laboratory is a Federally Funded Research and Development Center (FFRDC) sponsored by the National Cancer Institute (NCI) and operated by Leidos Biomedical Research,…

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Bump hg38 VEP version to 94

Ensembl Variant Effect Predictor (VEP) VEP determines the effect of your variants (SNPs, insertions, deletions, CNVs or structural variants) on genes, transcripts, and protein sequence, as well as …… Read more > The GA4GH Variation Representation Specification (4) Context precision: VRS does not lump together or link variation contexts; a variation…

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chromosome start & end for genes in biomart R not showing

chromosome start & end for genes in biomart R not showing 1 I have a list of genes which i want to get their cordinates and ref-seq ids but “start” & “end” attributes in biomart keeps throwing error. could you please take a look? library(biomart) #define which database to use…

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Availability of information on genes in Gnomad VCF data

Availability of information on genes in Gnomad VCF data 1 Hi , Im new to gnomad and genetics in general and i was wondering does the gnomad genome data that is downlaoded in the vcf format on variants contains information of what is the nearest gene and is the genomic…

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Analysis of merged transcriptomic and genomic datasets to identify genes and pathways underlying residual feed intake in growing pigs

Ellis, E. C. et al. Anthropogenic transformation of the biomes, 1700 to 2000. Glob. Ecol. Biogeogr. 19(5), 589–606 (2010). Google Scholar  Soleimani, T., Hermesch, S. & Gilbert, H. Economic and environmental assessments of combined genetics and nutrition optimization strategies to improve the efficiency of sustainable pork production. J. Anim. Sci….

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Add microRNA annotation to DEseq2

Add microRNA annotation to DEseq2 0 Hello, I am trying to perform micro-RNA analysis and convert ENSEMBL IDs to microRNA I tried using Biomart and results are attached. It doesn’t produce any micro-RNA. Why is this happening and is there any other way to annotate micro-RNA into the pipeline like…

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Doubt on annotation of ensembl ids in biomart using R

Doubt on annotation of ensembl ids in biomart using R 0 Hi All 🙂 I have a large list of unique ENSEMBL IDs (48,432) representing mouse genes, my goal only is to get attributes like gene name and ncbi id to build a dataset with expression data. However when using…

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Assessing spermatozoal small ribonucleic acids and their relationship to blastocyst development in idiopathic infertile males

Vander, B. M. & Wyns, C. Fertility and infertility: Definition and epidemiology. Clin. Biochem. 62, (2018). Sun, H. et al. Global, regional, and national prevalence and disability-adjusted life-years for infertility in 195 countries and territories, 1990–2017: Results from a global burden of disease study, 2017. Aging 11, 10952 (2019). Article …

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metY SAM V (53-MER) from Candidatus Pelagibacter ubique HTCC1062 (PDB 6FZ0, chain A)

Javascript is currently disabled or is not supported by this browser. Please enable JavaScript for full functionality. Sorry, there was a problem loading sequence from server. Please try again and contact us if the problem persists. Automated summary: This ncRNA sequence is 53 nucleotides long and is found in Candidatus…

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microarray analysis – gene upregulation/downregulation

microarray analysis – gene upregulation/downregulation 0 Hi guys, I have performed microarray differential expression analysis using the following R commands/script: library(“arrayQualityMetrics”) > library(GEOquery) > library(oligo) > library(Biobase) > library(affy) > library(“splitstackshape”) > library(“tidyr”) > library(dplyr) > celFiles <- list.celfiles() > affyRaw <- read.celfiles(celFiles) Platform design info loaded. Reading in :…

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Bioinformatics Analyst II Job in Maryland (MD), Research and Development Career, Full Time Jobs in Frederick National Laboratory for Cancer Research

Bioinformatics Analyst II Job ID: req3154Employee Type: exempt full-timeDivision: Applied & Development Research ProgramFacility: Frederick: Ft DetrickLocation: PO Box B, Frederick, MD 21702 USA The Frederick National Laboratory is a Federally Funded Research and Development Center (FFRDC) sponsored by the National Cancer Institute (NCI) and operated by Leidos Biomedical…

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Extra-hematopoietic immunomodulatory role of the guanine-exchange factor DOCK2

Cell isolation, reprogramming and culture Approval was obtained for human cell and tissue sample collection and genetic reprogramming from the Institutional Review Board (protocols 19–252, 18–243, 21–060, 19–284 and 415-E/1776/4-2014, Ethics Committee of the province of Salzburg). Adult samples were collected in accordance with the Declaration of Helsinki after written…

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Nomascus leucogenys (northern white-cheeked gibbon) nle-miR-34a

Javascript is currently disabled or is not supported by this browser. Please enable JavaScript for full functionality. Sorry, there was a problem loading sequence from server. Please try again and contact us if the problem persists. Nomascus leucogenys (northern white-cheeked gibbon) nle-miR-34a URS000030BD69_61853 Automated summary: This miRNA sequence is 22…

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Nociceptor neurons affect cancer immunosurveillance

Secondary use of biopsies as research specimens The ten melanoma samples used in this study were collected by Sanford Health and classified by a board-certified pathologist. Their secondary use as research specimens (fully de-identified formalin-fixed, paraffin-embedded (FFPE) blocks) was approved under Sanford Health IRB protocol 640 (titled ‘Understanding and improving…

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Genome-wide identification of disease-causing copy number variations in 450 individuals with anorectal malformations

CNV analysis After the application of quality filter step I (gender mismatch), two patients were excluded. Through step II (call rate <98%), 40 patients were discarded and after step III (exceeded double of standard deviation), 12 more patients were excluded. In the remaining 396 individuals with ARM, a total of…

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Phenotypic plasticity and genetic control in colorectal cancer evolution

Sample preparation and sequencing The method of sample collection and processing is described in a companion article (ref. 23). Sequencing and basic bioinformatic processing of DNA-, RNA- and ATAC-seq data are included there as well. Gene expression normalization and filtering The number of non-ribosomal protein-coding genes on the 23 canonical chromosome pairs…

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Is it okay to use dna.toplevel file for alignment if primary assembly file is not available?

Is it okay to use dna.toplevel file for alignment if primary assembly file is not available? 0 I cannot fine the primary assembly file for glycine max on ensembl plants. Can I use dna.top.level file or should i just stick to the sm files? I have read somewhere that “If…

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Empathy List Archives

Dear colleagues, Data on sequence level mapping through the SIFTS process www.ebi.ac.uk/pdbe/docs/sifts/ can now be retrieved directly from updated mmCIF files, available on PDBe entry pages. These files now contain residue-level mapping between UniProt and PDB entries, including coordinate numbering for UniProt accessions and mapping to a host of other…

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Microarray DEG scatterplot

Hi, I have found that my selected gene, probe I.D 201667_at is differentially expressed between WDLPS and DDLPS tumour tissue samples after performing microarray DEG analysis. Instead of just a p value in a table format: Probe I.D “201667_at” logFC 10.8205874181535 AveExpr 10.6925705768407 t 82.8808890739766 P.Value 3.10189446528995e-88 adj.P Val 3.10189446528995e-88…

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How can I convert Ensembl ID to gene symbol in R?

I tried several R packages (mygene, org.Hs.eg.db, biomaRt, EnsDb.Hsapiens.v79) to convert Ensembl.gene to gene.symbol, and found that the EnsDb.Hsapiens.v79 package / gene database provides the best conversion quality (in terms of being able to convert most of Ensembl.gene to gene.symbol). Install the package if you have not installed by running…

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Live-seq enables temporal transcriptomic recording of single cells

Biological materials RAW264.7, 293T and HeLa cells were obtained from ATCC. RAW264.7 cells with Tnf-mCherry reporter and relA-GFP fusion protein (RAW-G9 clone) were kindly provided by I.D.C. Fraser (National Institutes of Health). The IBA cell line derived from the stromal vascular fraction of interscapular brown adipose tissue of young male…

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Installing ensembl-vep

Installing ensembl-vep 1 Hello all , I tried installing ensembl-Vep on mu ubuntu using following commands git clone github.com/Ensembl/ensembl-vep cd ensembl-vep perl INSTALL.pl I got the error as :- ERROR: DBI module not found. VEP requires the DBI perl module to function I tried installing DBI etc but the error…

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Patrick Murphy Bulk RNA-Seq – HackMD

Patrick Murphy Bulk RNA-Seq – HackMD        owned this note   Published Linked with GitHub — title: ‘Patrick Murphy Bulk RNA-Seq’ disqus: hackmd — Patrick Murphy bulk RNA-Seq Analysis === ## Table of Contents [TOC] ## 1. Introduction This is a bulk RNA-Seq project, which includes human data….

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hgmd variants from Ensembl

hgmd variants from Ensembl 0 I know that Ensembl has hgmd variants amongst other annotations as given here: hgmd variants How can I download these variants from Ensembl? I downloaded VEP because it uses multiple tracks including HGMD: VEP Is it possible to use VEP to extract just the HGMD…

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Error in Create switchAnalyzeRlist

Error in Create switchAnalyzeRlist 0 Hello everyone., I am using isoformswitchinganalysisR to look at different transcript expression and quantification data generated using Kallisto. I have stuck in step of Create switchAnalyzeRlist. According to warning error it looks I have problem in gtf file. Really appreciate anybody can help me how…

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In vivo transomic analyses of glucose-responsive metabolism in skeletal muscle reveal core differences between the healthy and obese states

Animals and sample preparation Animal experiments were performed as previously described12. C57BL/6J WT mice or ob/ob mice at ten weeks of age were purchased from Japan SLC Inc. (Shizuoka, Japan). The phenotypic data of the mice are summarized in Table S1. Animal experiments were approved by the animal ethics committee…

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Genomic architecture of adaptive radiation and hybridization in Alpine whitefish

Sampling the radiation To understand the phylogenetic relationships between Alpine whitefish, we carried out whole-genome resequencing on 96 previously collected whitefish (with associated phenotypic measurements including standard length and gill-raker counts; collected in accordance with permits issued by the cantons of Zurich (ZH128/15), Bern (BE68/15), and Lucerne (LU04/14); these fish…

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Ensembl ID mapping GRCh37 vs GRCh38

Ensembl ID mapping GRCh37 vs GRCh38 0 I currently have a large list of Ensembl protein IDs (ENSP) that are from GRCh37. I need to map these IDs to the entry name listed on the UniProt website (e.g. ‘CASPE_HUMAN’ ). I am having trouble doing this using the UniProt dataset…

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EnWik > Expasy

Expasy is an online bioinformatics resource operated by the SIB Swiss Institute of Bioinformatics. It is an extensible and integrative portal which provides access to over 160 databases and software tools and supports a range of life science and clinical research areas, from genomics, proteomics and structural biology, to evolution…

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Get gene names from rs SNP ids for mmusculus_gene_ensembl

Get gene names from rs SNP ids for mmusculus_gene_ensembl 1 I have mouse data with an old mapping from rs SNP IDs to base pair position and chromosome. I would like to query Ensembl to get the genes and then the gene annotations for the rs SNP IDs in my…

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r – Alternative Biomart Hosts

I am trying to run the following Biomart script (params$species == “rabbit”){ ensembl = useMart(biomart=”ENSEMBL_MART_ENSEMBL”, dataset=”ocuniculus_gene_ensembl”, host=”uswest.ensembl.org“, ensemblRedirect = FALSE) orgSymbols <- unlist(getBM(attributes=”ensembl_gene_id”, mart=ensembl)) mart <- useMart(dataset = “ocuniculus_gene_ensembl”, biomart=”ensembl”, host=”uswest.ensembl.org“) The host keeps timing out – does anyone know another host URL I could use? Thank you Read more…

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Uncharted genetic territory offers insight into human-specific proteins

When researchers working on the Human Genome Project completely mapped the genetic blueprint of humans in 2001, they were surprised to find only around 20,000 genes that produce proteins. Could it be that humans have only about twice as many genes as a common fly? Scientists had expected considerably more….

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Scientist I, Bioinformatics job at Vir Biotechnology in United States – 53516780

Vir Biotechnology is a commercial-stage immunology company focused on combining immunologic insights with cutting-edge technologies to treat and prevent serious infectious diseases. Vir has assembled four technology platforms that are designed to stimulate and enhance the immune system by exploiting critical observations of natural immune processes. Its current development pipeline…

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Unexprected Ensembl-vep results

Unexprected Ensembl-vep results 0 Hi.I got a VCF from an individual that shows symptoms of a known disease with known mutations. I run it with Ensembl-vep, expecting to find some of those mutations in the results, yet, all the consequences in the results are “intergenic-variant”.The command I used was: –cache…

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Table 5 | Scripting Analyses of Genomes in Ensembl Plants

arabidopsis_thaliana The 1001 Genomes Project [18] arabidopsis_thaliana Nordborg [19] brachypodium_distachyon Jaiswal_lab_OSU [20] hordeum_vulgare International Barley Sequencing Consortium (IBSC) [21,22,23] hordeum_vulgare Ensembl Plants [24] hordeum_vulgare Illumina iSelect SNP chip [22] malus_domestica fruitbreedomics.com [25] oryza_glaberrima Glab (OGE) oryza_glaberrima Barthii (OGE) oryza_glumipatula Oryza Genome Evolution (OGE) oryza_indica dbSNP [26] oryza_sativa www.ebi.ac.uk/eva [27,28,29,30] oryza_sativa…

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Allelic expression imbalance of PIK3CA mutations is frequent in breast cancer and prognostically significant

Subjects Normal breast and tumor samples were obtained with the written informed consent from donors and appropriate approval from local ethical committees, with the detailed information described in the respective original publications: normal tissue9, METABRIC14, TCGA35. Differential allelic expression analysis DNA and total RNA from 64 samples of normal breast…

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Supporting African BioGenome Project to build bioinformatics capacity

The African BioGenome Project and EMBL have signed a Memorandum of Understanding to support each other in building genomics and bioinformatics capacity across Africa AfricaBP and EMBL MoU. Credit: Karen Arnott/EMBL-EBI The African BioGenome Project (AfricaBP) is an African-led effort to sequence the genomes of all species indigenous to the…

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Somatic point mutations are enriched in non-coding RNAs with possible regulatory function in breast cancer

Torre, L. A., Siegel, R. L., Ward, E. M. & Jemal, A. Global cancer incidence and mortality rates and trends—an update. Cancer Epidemiol. Prev. Biomark. 25, 16–27 (2016). Article  Google Scholar  Gerasimova, E. et al. Wavelet-based multifractal analysis of dynamic infrared thermograms to assist in early breast cancer diagnosis. Front….

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Combining SNP-to-gene linking strategies to identify disease genes and assess disease omnigenicity

Visscher, P. M. et al. 10 years of GWAS discovery: biology, function, and translation. Am. J. Hum. Genet. 101, 5–22 (2017). CAS  PubMed  PubMed Central  Google Scholar  Buniello, A. et al. The NHGRI-EBI GWAS Catalog of published genome-wide association studies, targeted arrays and summary statistics 2019. Nucleic Acids Res. 47,…

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RPKM threshold estimation – SEQanswers

Dear All, I have a doubt in the calculation of False postitive rate while checking for FPKM threshold in a RNAseq experiment. Following the method previously published (www.ploscompbiol.org/article/…l.pcbi.1000598). I am not getting desired results. I followed the method as mentioned the publication Reads were mapped to Ensembl genes (blue) and…

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xml – I am trying to importxml from ENSEMBL using xpath copied from Chrome, but it is not working

I want to use google sheet’s IMPORTXML to extract the gene name (SLC3A1) and ensembl ID (ENSG00000138079) from this URL: asia.ensembl.org/Multi/Search/Results?q=SLC3A1;site=ensembl I tried copying xpath from Chrome and also tried deriving it on my own step by step, but I am only getting a #NA. My xpath: /html/body/div[1]/div/div[2]/div[1]/div[1]/div[2]/div[2]/div[4]/div/div/div[2]/div/div/div[3]/div[2]/div[1]/div[2]/div/div/div/div[1]/div/div[2]/span From Chrome:…

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How to analyze the differential expression of a gene if it has several ensembl id

How to analyze the differential expression of a gene if it has several ensembl id 0 Hie, I have obtained the differential expressions of genes (from RNA-seq data) in an excel file. I can see one gene has several ensembl ids and I guess the gene is from the haplotype…

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Transcription Start Site

Transcription Start Site 2 What are the best databases to check out the transcription start sites of specific genes in human genome? TSS • 130 views wget -q -O – “http://hgdownload.cse.ucsc.edu/goldenPath/hg19/database/wgEncodeGencodeBasicV19.txt.gz” | gunzip -c | awk ‘(int($7)< int($8)) {if($4==”+”) {printf(“%s\t%d\t%d\t%s\t%s\n”,$3,$7,int($7)+1,$2,$4);}else {printf(“%s\t%d\t%d\t%s\t%s\n”,$3,int($8)-3,$8,$2,$4);}}’ chr1 69090 69091 ENST00000335137.3 + chr1 139306 139309 ENST00000423372.3…

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Bioconductor – AHEnsDbs

DOI: 10.18129/B9.bioc.AHEnsDbs     This package is for version 3.10 of Bioconductor; for the stable, up-to-date release version, see AHEnsDbs. EnsDbs for AnnotationHub Bioconductor version: 3.10 Supplies AnnotationHub with EnsDb Ensembl-based annotation databases for all species. EnsDb SQLite databases are generated separately from Ensembl MySQL databases using functions from the…

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snp – Reference variant detected as altered one in bam file

I received (from manufacturer) several .bam files and I used four callers (samtools, freebayes, haplotypecaller, deepvariant) to find some sequence variants. In obtained .vcf files, I took a closer look to some calls. I found interesting, homozygous one rs477033 (C/G Ref/Alt) with flag ‘COMMON=0’ and very low MAF. I also…

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Antiplatelet response to clopidogrel is associated with a haplotype in CYP2C19 gene in Pakistani patients

Amsterdam, E. A. et al. 2014 AHA/ACC guideline for the management of patients with non-ST-elevation acute coronary syndromes: Executive summary: A report of the American College of Cardiology/American Heart Association Task Force on Practice Guidelines. Circulation 130, 2354–2394 (2014). Google Scholar  Kernan, W. N. et al. Guidelines for the prevention…

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ENHANCED GRAVITROPISM 2 encodes a STERILE ALPHA MOTIF–containing protein that controls root growth angle in barley and wheat

    Significance To date, the potential of utilizing root traits in plant breeding remains largely untapped. In this study, we cloned and characterized the ENHANCED GRAVITROPISM2 (EGT2) gene of barley that encodes a STERILE ALPHA MOTIF domain–containing protein. We demonstrated that EGT2 is a key gene of root growth…

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How to map old Ensembl Gene IDs to HGNC symbols and Entrez IDs

How to map old Ensembl Gene IDs to HGNC symbols and Entrez IDs 0 I have a list of Ensembl version 74 gene IDs that I need to convert to HGNC symbols. I was wondering what the best way to go about this would be? Originally I used biomaRt with…

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Easy Way To Get 3′ Utr Lengths Of A List Of Genes

Easy Way To Get 3′ Utr Lengths Of A List Of Genes 4 Hi, as the title says really, I’m wondering if there is any tool available that would allow me to drop in a list of say entrez gene ids and get their corresponding 3′ UTR lenghts? Thanks for…

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List of human protein coding genes with given name (known function?)

List of human protein coding genes with given name (known function?) 2 Hello, To put it simply, I am doing differential expression analysis on human RNA-seq data and I want to focus my analysis of genes that are: 1) Protein coding, so no SNOR or MIR 2) Genes with a…

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Novogene America hiring Bioinformatics Specialist in Sacramento, California, United States

Job description Responsibilities: · Develop and maintain bioinformatics pipeline of NGS data · Research in Bioinformatics Specialty and Follow up the Frontier Trends of Life Science Research · Data mining from high throughput sequencing data generated by Novogene or other research groups. · Responsible for the maintain and improvement of…

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Head of Bioinformatics job in Princeton, NJ | GENMAB A/S

Genmab is focused on the creation and development of innovative and differentiated antibody products, with the aim of improving the lives of cancer patients. The Role The successful candidate will lead the global bioinformatics function and be responsible for many aspects of data including architecture, access, classification, standards, integration, pipelines…

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VEP plugin uses

VEP plugin uses 0 It is a small question, I downloaded all plugins and wrote in the input script to get plugin experimental functionalities (I am using merged cache Ref_Seq & Ensembl both). # make a file with a single variant using bash echo “17 43071077 43071077 T/C + variant_1″…

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Mapping unique GO term description given a specific GO id

Mapping unique GO term description given a specific GO id 0 I have a list of GO ids and I want to find unique term description such that if I provide say 200 GO IDs I will give 200 specific GO terms. The code snippet I am using is given…

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cannot write to FASTA lockfile

Ensembl VEP Docker: cannot write to FASTA lockfile 0 Hello, I am trying to use the Ensembl VEP tool to annotate a database after liftover. For ease of use (or so I thought) I followed all the necessary steps decribed in the official documentation. I want to use local cache…

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Ribon Therapeutics hiring Principal Scientist Bioinformatics in Cambridge, MA, US

About Ribon Therapeutics Ribon is a clinical-stage biotechnology company dedicated to the discovery and development of first-in-class small molecule inhibitors to block the fundamental ability of cancer cells to survive under stress. Job Description Ribon Therapeutics, a clinical biopharmaceutical company focused on targeting stress pathways to develop novel cancer therapeutics,…

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Bioconductor Forum

James W. MacDonald 57k 1 week, 5 days ago United States Answer: Biomart’s getBM returns no genes for an existing GO-term in grch38, and less the Michael Love 33k 1 week, 6 days ago United States Answer: Normalizing 5′ Nascent RNA-seq data to identify differentially expressed transcr Kevin Blighe 3.3k 2 weeks, 2 days ago Republic…

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Interpreting dbNSFP prediction scores

Interpreting dbNSFP prediction scores 1 How should I interpret for example, SIFT score: SIFT_pred = T;T;T;T;D;T SIFT_score = 0.138;0.138;0.138;0.138;0.042;0.157 From dbNSFP documentation, I understand the meaning of D and T D: Deleterious (sift<=0.05); T: tolerated (sift>0.05) My question is: Why there are multiple values of both pred and score in…

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working with .gmt files

working with .gmt files 3 Hi! I have downloaded a pathway data set in .gmt format form the GSEA website. I’m wondering how can I properly read this data set in R. Could anyone help me? Thank you!   myposts • 9.5k views • link updated 2 hours ago by…

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Bio-DB-HTS installation and ensembl-vep

Bio-DB-HTS installation and ensembl-vep 0 I want to use ensembl-vep with custom annotation. In order to use gff file I need to have library Bio-DB-HTS installed. I downloaded Bio-DB-HTS and used Build.PL with no errors. When I try to install ensembl-vep it still gives an error asking for Bio-DB-HTS library….

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Looking up Gene IDs in R

Looking up Gene IDs in R 1 Hello, Given a list of gene names, I need to create a table containing the Ensemble ID, chromosome, start, end of that gene. Example: ## ens_id gene view chr start end ## 1: ENSG00000243485 MIR1302-2HG Gene Expression chr1 29553 30267 ## 2: ENSG00000237613…

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Sequence (annotation) databases in 2021

Forum:Sequence (annotation) databases in 2021 1 Hi everyone, So I know there are several threads on this topic already (or tangentially related to it). For example: But these threads are really old now. Things have probably changed quite significantly in the mean time. So I would like to start a…

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What is the difference between GRCh37 and hs37? And hg19?

This is what I have found so far. Please correct me if I am wrong. GRCh37 w/o patches includes the primary assembly (22 autosomal, X. Y, and non-chromosomal supecontigs) and alternate scaffolds, but not a reference mitogenome. Non-chromosomal supercontigs are the unlocalized and unplaced scaffolds. The rCRS reference mitogenome in…

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error while using plant ensembl biomart

error while using plant ensembl biomart 2 Hi, I am trying to extract gene ontology term from plant ensembl biomart using the following code: from pybiomart import Server server = Server(host=”http://plants.ensembl.org”) #print server.list_marts() # available marts mart = server[‘plants_mart’] # connecting plants_mart #print mart.list_datasets() # print available datasets dataset =…

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Convert unigene ID to ensembl ID

Convert unigene ID to ensembl ID 3 Hi Is there a way to convert UniGene ID to ensembl id? using python (my preference) or R? some Unigene ID: Hs.509736 Hs.467701 Hs.138860 Hs.124611 Hs.460988 Hs.49787 Hs.412707 Hs.43697 Hs.447458 Hs.436618 Hs.465087 Hs.439552 Hs.183428 Hs.219140 Hs.153863 Hs.181163 Hs.499891 Hs.410037 Hs.519601 Hs.75640 Hs.450230 Hs.133892…

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How to extract ancestral sequence for Cow from 46 eutherian mammals EPO Ensembl?

How to extract ancestral sequence for Cow from 46 eutherian mammals EPO Ensembl? 1 Hello everyone, I am trying to figure out how to extract the ancestral sequence of the bovine species from an Ensembl multiple alignment file: 46 eutherian mammals EPO. In the .emf files, I can see that…

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Biostar Systems

Comment: STAR vs Novoalign IGV Browser visualization by chasem &utrif; 10 That is good to know that it isn’t just my set of reads…still concerning, though. Comment: STAR vs Novoalign IGV Browser visualization by chasem &utrif; 10 I was not expecting this — not sure what to make of it…

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IOError [errno 2] No such file or directory: ‘-o’

TopHat error: IOError [errno 2] No such file or directory: ‘-o’ 2 Hello everyone. I’m now running tophat in our server. First, I just simply tried “tophat -p 8 -G <gtf_file> <ref_genome> <fa1><fa2>” and it worked. Then I wrote a for loop scripts but It reported error: [2019-04-03 10:49:16] Beginning…

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Rancho BioSciences hiring RShiny Scientist/Bioinformatics in San Diego, California, United States

Rancho Biosciences is seeking a RShiny bioinformatics expert to provide support for a range of projects which include the development of R-Shiny applications, managing NGS data associated with clinical and metadata organization. Work from home while supporting high impact pharmaceutical, academic and institutional science programs. We are specifically looking for…

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Eukaryotic Annotation Team Leader job with EMBL

We are seeking a faculty-level genome scientist to lead the Eukaryotic Annotation Team at the European Molecular Biology Laboratory’s European Bioinformatics Institute (EMBL-EBI).  As Team Leader you will have strategic responsibility for gene annotation and comparative genomics in the Ensembl project, and also lead the EMBL contribution to a portfolio…

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Answer: PopGenome – VCF, fasta, GTF and codons still missing

Dear Maciek Hopefully you were able to solve these problems already. I cannot comment on the main set of issues you reported. However, I also encountered the error: `Error in START[!REV, 3] : incorrect number of dimensions` following certain instances of `set.synnonsyn` which I also noticed occurred for genes which…

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a specific transcript sequence from transcriptome mapped RNA seq

a specific transcript sequence from transcriptome mapped RNA seq 0 hi, i want to start working on a gene for which more than 10 splice varients are shown on ensembl. Fortunately i have access to the BAM file of transcriptome mapped, using STAR, RNA seq data from the cells which…

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How to trim a GFF3 file based on specific coordinates?

How to trim a GFF3 file based on specific coordinates? 0 Hi, I would like to create a GFF3 file containing information only for specific coordinates from the chromosome level GFF3 file. I know how to extract gene and CDS info separately but don’t know how to do trimming based…

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Inquiry related to vcf file and formatting

Hello everyone, I am trying to run predixcan software. But its showing error as segmentation fault implying that there is something wrong with my vcf files. I am sharing the header of vcf file. ##fileformat=VCFv4.1 ##INFO=<ID=LDAF,Number=1,Type=Float,Description=”MLE Allele Frequency Accounting for LD”> ##INFO=<ID=AVGPOST,Number=1,Type=Float,Description=”Average posterior probability from MaCH/Thunder”> ##INFO=<ID=RSQ,Number=1,Type=Float,Description=”Genotype imputation quality from…

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Antibody Matching Transcripts

Antibody Matching Transcripts 1 Hello everyone, I am interested to find the matching isoforms of a gene that my HPA antibody is able to hit. When I check the Human Atlas Protein website it gives me a list of 4 transcripts my antibody can recognize. However, when I open ensembl…

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Converting mouse Gene IDs to Human while keeping genes that don’t convert

Hi there, I am using bioMart to convert some gene IDs from mouse to human for some data I generated through RNA-seq. I am currently mapping using the following function: convertMouseGeneList <- function(x){ require(“biomaRt”) human = useMart(“ensembl”, dataset = “hsapiens_gene_ensembl”) mouse = useMart(“ensembl”, dataset = “mmusculus_gene_ensembl”) genesV2 = getLDS(attributes =…

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How to search dbSNP using a list of SNPs and retrieve Gene name (hgnc symbol if existing, otherwise just whatever is in there)

How to search dbSNP using a list of SNPs and retrieve Gene name (hgnc symbol if existing, otherwise just whatever is in there) 2 I have a list of 500.000 SNPs from which I want to obtain the gene name. I try to search with biomaRt library(data.table) library(biomaRt) rs <-…

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microarray miRNA expression data analysis

I wrote a script on how to analyze the microarray-based miRNA expression data. Here is my code: # general config baseDir <- ‘.’ annotfile <- ‘mirbase_genelist.tsv’ setwd(baseDir) options(scipen = 99) require(limma) # read in the data targets <- read.csv(“/media/mdrcubuntu/46B85615B8560439/microarray_text_files/targets.txt”, sep=””) # retain information about background via gIsWellAboveBG project <- read.maimages(targets,source=”agilent.median”,green.only…

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Answer: AnnotationHub::mapIds() cannot find existing ENSG (GEO supplemental data cross-r

Hi, a quick check on NCBI Gene reveals that the official symbol for this is *PRXL2C*, not *AAED1*. In this way, I would not have expected `org.Hs.eg.db` (using ‘recent’ annotation) to have it. However, I can see that `EnsDb.Hsapiens.v86` (older version) does [have it]. So, there must have been an…

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AnnotationHub::mapIds() cannot find existing ENSG (GEO supplemental data cross-referenced with ensembl.org)

Anyone know why I’m not getting ENSG ids for some of these symbols? The example below retrieves `NA` for multiple symbols, including AAED1 [whose ENSG is ENSG00000158122][1]. “` > library(AnnotationHub) > library(org.Hs.eg.db) > library(GEOquery) > temp download.file(getGEO(“GSM4430459″)@header$supplementary_file_1,temp) > genes unlink(temp) > ensids = mapIds(org.Hs.eg.db, keys=genes, column=”ENSEMBL”, keytype=”SYMBOL”, multiVals=”first”) > ensids[“AAED1”]…

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Why does Txdb transcript length not always match to transcript end-start position?

Why does Txdb transcript length not always match to transcript end-start position? 2 I have just found an example that biomart’s transcript_length is not identical with transcript_end – transcript_start. ensembl_gene_id mgi_symbol chromosome_name strand start_position end_position gene_biotype transcript_start transcript_end strand.1 transcript_length 128537 ENSMUSG00000037860 Aim2 1 1 173178445 173293606 protein_coding 173178445 173293606…

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install ensembl-vep

install ensembl-vep 0 Hello, I want to install ensembl-vep in my Ubuntu 18.04.2. I have already installed LWP::Simple. What can I do in the next step? Thanks in advance for great help! Best, Yue Inspiron-3670:~$ perl -MCPAN -e’install “LWP::Simple”‘ Reading ‘/home/jing/.cpan/Metadata’ Database was generated on Sat, 07 Aug 2021 06:55:53…

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Where To Find Annotation File For Agilent Microarray?

An easier way that has [probably] only come about since this question was posted is via biomaRt in R. You can build annotation tables for Agilent 4×44 arrays for mouse and human as follows: require(biomaRt) Homo sapiens # agilent_wholegenome_4x44k_v1 mart <- useMart(‘ENSEMBL_MART_ENSEMBL’) mart <- useDataset(‘hsapiens_gene_ensembl’, mart) annotLookup <- getBM( mart…

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