Tag: GBS

Group B Streptococcus Cas9 variants provide insight into programmable gene repression and CRISPR-Cas transcriptional effects

Amino acid sequence comparisons between GAS and GBS Cas9 identify orthologous active sites Relationships between the amino acid sequence of GBS Cas9 endonuclease and its molecular functions can be deduced from detailed studies of its GAS ortholog, SpyCas9 (Supplementary Fig. 1). As a first step in locating DNA complementary to a…

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Microbial diversity in the vaginal microbiota and its link to pregnancy outcomes

A prospective study of pregnant women We established a prospective cohort of 2313 pregnant women with the goal of studying the vaginal microbiota, its community composition and the presence of pathogenic bacteria in the peripartum period. Inclusions and data collection were performed in accordance with applicable laws and ethical standards. Ethical…

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Get gradient of quantum circuit with PyTorch interface – PennyLane Help

Hello! I’m trying to get the gradient values of my quantum circuit to do a Barren Plateaus analysis. I am following the following tutorial: Barren plateaus in quantum neural networks — PennyLane documentation. However, I am using the torch interface, and I am running into a TypeError when I just…

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What Are The Most Common Stupid Mistakes In Bioinformatics?

Forum:What Are The Most Common Stupid Mistakes In Bioinformatics? 78 While I of course never have stupid mistakes…ahem…I have many “friends” who: forget to check both strands generate random genomic sites without avoiding masked (NNN) gaps confuse genome freezes and even species but I’m sure there are some other very…

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Microsatellite markers reveal genetic diversity and population structure of Portunus trituberculatus in the Bohai Sea, China

Liu, L. et al. Identification of quantitative trait loci for growth-related traits in the swimming crab Portunus trituberculatus. Aquacult. Res. 46, 850–860 (2015). Article  CAS  Google Scholar  Sun, Q. F. et al. Astaxanthin: The ubiquitous and abundant carotenoid as a pivotal interior factor of anti-oxidation and immune for the moulting…

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Barren habitat for sows leaves imprint on piglets’ brains

Experimental design. Sows were maintained in an enriched (a) or barren environment (b). Sows kept in the barren environment were subdivided into two groups (c) depending on whether they expressed (BS) or did not express stereotypy (BN). Piglets from different prenatal environments were kept in the same conditions after weaning,…

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The wheat stem rust resistance gene Sr43 encodes an unusual protein kinase

Mutant collection development We mutagenized 2,700 seeds of the wheat–Th. elongatum introgression line RWG34 containing Sr43 (ref. 29). Dry seeds were incubated for 16 h with 200 ml of a 0.8% (w/v) EMS solution with constant shaking on a Roller Mixer (Model SRT1, Stuart Scientific) to ensure maximum homogenous exposure of the…

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Amplification Biases and Consistent Recovery of Loci in a Double-Digest RAD-seq Protocol | PLOS ONE – Molecular Biology Protocol – Restriction Digest of Plasmid …

Abstract A waxing variety of “genotype-by-sequencing” (GBS) methods use restriction enzymes and high throughput DNA ordering to generation data for a set of genomic loci, allowing the simultaneous discovery press genotyping of thousands of polymorphisms in a set of multiplexed samples. We evaluated a “double-digest” restriction-site associated DNA sequencing (ddRAD-seq)…

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Moderna Reports First Quarter 2023 Financial Results and Provides Business Updates

First quarter 2023 revenues of $1.9 billion; GAAP net income of $79 million and GAAP diluted EPS of $0.19 Company in negotiations for new orders for fall of 2023 in U.S., Japan, and EU, in addition to reiterating 2023 minimum sales expectations of approximately $5.0 billion from previously announced COVID-19…

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Cutadapt in Omicsbox: Demultiplexing and Barcoding

Barcoding and demultiplexing in high-throughput sequencing experiments Barcoding, or indexing, is a widely used strategy in high-throughput sequencing experiments. This method enables the multiplexing of numerous samples in a single sequencing run by adding a unique DNA sequence to each sample before sequencing. During sequencing, the barcodes are read along…

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Thermophilic Dehalococcoidia with unusual traits shed light on an unexpected past

Two new Tepidiforma species expand on a small number of cultivated Dehalococcoidia Two bacterial strains were isolated from terrestrial geothermal springs by plating benthic mat and sediment slurries onto R2A medium and were identified by 16S rRNA gene sequencing as members of the recently described genus Tepidiforma [27]. Strain YIM…

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Moderna Announces Clinical and Program Updates at 4th Vaccines Day

Next-generation, refrigerator-stable COVID-19 vaccine candidate, mRNA-1283, has dosed first participant in its Phase 3 trial Company expects to file for approval of its investigational RSV vaccine candidate, mRNA-1345, this quarter Company’s first influenza candidate, mRNA-1010, did not accrue sufficient cases at the interim efficacy analysis to declare early success in…

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LOINC 5034-4 Streptococcus agalactiae rRNA [Presence] in Specimen by Probe

zh-CN Chinese (China) 无乳链球菌 rRNA:存在情况或阈值:时间点:XXX:序数型:DNA探针Synonyms: B 族链球菌 rRNA B 族链球菌;B 组链球菌;B 群链球菌;GBS B 组链球菌 rRNA B 群链球菌 rRNA 不明的;其他;将在相应消息内其他部分之中加以详细说明;未作详细说明的;未作说明的;未做说明的标本;未加规定的;未加说明的标本;杂项 依次型;分类顺序型;定性的;序数型(或称等级型);性质上的;有序型;有序性分类应答;有序性分类结果;秩次型;等级型;筛查;顺序型 存在情况;存在;存在与否;是否存在;阈值;界值;界限;阀值;临界值;存在情况(存在、存在与否、是否存在)或阈值(界值、界限、阀值、临界值) 微生物学;微生物学试验;微生物学试验(培养、DNA、抗原及抗体) 时刻;随机;随意;瞬间 核糖体核糖核酸;核糖核酸 RNA 核蛋白体 RNA 核蛋白体 RNA(Ribosomal RNA,RRNA) 核蛋白体核糖核酸 核醣体 RNA 脱氧核糖核酸探针 es-AR Spanish (Argentina) ARNr de Streptococcus agalactiae:concentración arbitraria:punto en el tiempo:XXX:ordinal:sonda fr-CA French (Canada) Streptococcus agalactiae…

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MultiQC output: opposing output

MultiQC output: opposing output 1 Hi, I’m working with RAD genomic data (produced through genotype-by-sequencing). Two panels in my MultiQC document seem to contradict one another, the “Trimmed Sequence Lengths (3′)” and the “Sequence Length Distribution” panels. I expect a lot of duplication because of the GBS protocol. In the…

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Preadapted to adapt: underpinnings of adaptive plasticity revealed by the downy brome genome

Bradley, B. A. et al. Cheatgrass (Bromus tectorum) distribution in the intermountain western United States and its relationship to fire frequency, seasonality, and ignitions. Biol. Invasions 20, 1493–1506 (2018). Article  Google Scholar  Balch, J. K., Bradley, B. A., D’Antonio, C. M. & Gomez-Dans, J. Introduced annual grass increases regional fire…

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Is there a placental microbiota? A critical review and re-analysis of published placental microbiota datasets | BMC Microbiology

Principal findings of the study In this re-analysis of fifteen placental microbiota studies, of the ASVs which were ranked in the top five ASVs for relative abundance in any one study, Lactobacillus ASVs were clearly the most prevalent across studies. Yet, Lactobacillus ASV prevalence was explained by background DNA contamination,…

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What is Kaggle? – IoT Worlds

Kaggle is a platform for sharing ideas, getting inspired, competing against other data scientists and learning new information and coding tricks. Kaggle is free and open to the entire online community, featuring machine learning contests and data challenges as well as notebooks and datasets. Getting Started Kaggle is an ideal…

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FDA Advisory Committee Recommends Approving First-Ever RSV Vaccine

Today, the Vaccines and Related Biological Products Advisory Committee (VRBPAC) voted in favor of the safety and efficacy of Pfizer’s respiratory syncytial virus (RSV) vaccine candidate. If the US Food and Drug Administration (FDA) and Centers for Disease Control and Prevention (CDC) abide by this recommendation, the vaccine, Abrysvo (RSVpreF),…

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Rare neurological condition is ‘important potential risk’ of Pfizer’s RSV vaccine, FDA says

CNN — Two people who received Pfizer’s respiratory syncytial virus (RSV) vaccine during a clinical trial were later diagnosed with Guillain-Barre syndrome, and the US Food and Drug Administration has asked Pfizer to conduct a safety study if the shot is approved, according to agency documents released Friday. The cases…

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Genotyping by Sequencing (GBS) for Genome-Wide SNP Identification in Plants

Lande R, Thompson R (1990) Efficiency of marker-assisted selection in the improvement of quantitative traits. Genetics 124:743–756. doi.org/10.1093/genetics/124.3.743 CrossRef  CAS  Google Scholar  Rafalski JA, Tingey SV (1993) Genetic diagnostics in plant breeding: RAPDs, microsatellites and machines. Trends Genet 9:275–280. doi.org/10.1016/0168-9525(93)90013-8 CrossRef  CAS  Google Scholar  Ragot M, Hoisington DA (1993) Molecular…

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Arlequin – SNP outlier analysis help

I’m trying to identify SNP outliers on my GBS (genotype-by-sequencing) SNP dataset. I found “Arlequin” and have installed it successfully. Furthermore, I’ve converted my “VCF” file into “arp” which is the format needed for Arlequin. When I load my data into Arlequin and choose “Detecting loci under selection” and press…

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Culexarchaeia, A Novel Archaeal Class Of Anaerobic Generalists Inhabiting Geothermal Environments

Biogeography of Culexarchaeia. Sites from where Culexarchaeia MAGs were recovered and where 16S rRNA gene sequences have been detected. A full list of samples and associated metadata can be found in Supplementary File 8. Yellowstone map modified from (97), GBS picture modified from (18), and JZ picture modified from (25)….

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CRISPR/Cas12a-based assay for the rapid and high-sensitivity detection of Streptococcus agalactiae colonization in pregnant women with premature rupture of membrane

Background: Streptococcus agalactiae or group B Streptococcus (GBS) is a leading infectious cause of neonatal morbidity and mortality. It is essential to establish a robust method for the rapid and ultra-sensitive detection of GBS in pregnant women with premature rupture of membrane (PROM). Methods: This study developed a CRISPR-GBS assay…

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Senior Scientist bioinformatics at Eurofins Scientific – Bengaluru

Company Description Eurofins Scientific is an international life sciences company, providing a unique range of analytical testing services to clients across multiple industries, to make life and our environment safer, healthier and more sustainable. From the food you eat, to the water you drink, to the medicines you rely on,…

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Contrasting levels of hybridization across the two contact zones between two hedgehog species revealed by genome-wide SNP data

Ai H, Fang X, Yang B, Huang Z, Chen H, Mao L et al. (2015) Adaptation and possible ancient interspecies introgression in pigs identified by whole-genome sequencing. Nat Genet 47:217–225 CAS  PubMed  Article  Google Scholar  Alexander DH, Lange K (2011) Enhancements to the ADMIXTURE algorithm for individual ancestry estimation. BMC…

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Linkage mapping, comparative genome analysis, and QTL detection for growth in a non-model teleost, the meagre Argyrosomus regius, using ddRAD sequencing

Fricke, R., Eschmeyer, W. N. & van der Laan, R. (eds). Eschmeyer’s Catalog of Fishes: Genera, Species, Rererences. researcharchive.calacademy.org/research/ichthyology/catalog/fishcatmain.asp. Electronic version, Accessed 15 October 2021. Nelson, J. S. Fishes of the World 4th edn, 372 (Wiley, 2006). Google Scholar  Chen, X. H., Lin, K. B. & Wang, X. W. Outbreaks…

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Top 50 H20 interview questions and answers

H20 interview questions and answers 1) What is AutoML in H2O? H2O’s Automatic Machine Learning (AutoML) H2O is a fully open-source, distributed in-memory machine learning platform with linear scalability. … H2O AutoML can be used for automating the machine learning workflow, which includes automatic training and tuning of many models…

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Transposition and duplication of MADS-domain transcription factor genes in annual and perennial Arabis species modulates flowering

Annual and perennial species occur in many plant families. Annual plants and some perennials are monocarpic (flowering once in their life cycle), characterized by a massive flowering and typically produce many seeds before the whole plant senesces. By contrast, most perennials live for many years, show delayed reproduction, and are…

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Filter criteria for variants based on GBS data

Filter criteria for variants based on GBS data 0 Are there recommended filter criteria for variants based on GBS data? I currently use this filter formula that is used in bcbio for WGS based variants soft-filtering bcftools –soft-filter GATKCutoffSNP -e TYPE=”snp” && (MQRankSum < -12.5 || ReadPosRankSum < -8.0 ||…

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Beagle 5.2 Imputation Issue, understanding output

Hey everyone, I’m having some trouble with the imputation step in my GBS pipeline. I’m following the FastGBS pipeline basically as written up until this point. Before running Beagle, i’ve performed a filtering step using vcftools (though I get the same error whether or not I do this; I just…

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