Tag: GeCKO

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AllHigh-Throughput SequencingGenomicsProteomicsVisualizationOther MAGeCK Description (Model-based Analysis of Genome-wide CRISPR-Cas9 Knockout) a computational tool to identify important genes from the recent genome-scale CRISPR-Cas9 knockout screens (or GeCKO) technology. Installation Use the following command to install this title with the CLI client: $ biogrids-cli install mageck Copy to clipboard Primary Citation* W….

Significance The extracellular concentration of adenosine triphosphate (ATP) reaches several hundred micromoles in the inflamed tissues or tumor environment. A high concentration of ATP activates P2X7, a purinergic receptor, and induces the formation of a nonselective cation channel, accompanied by reversible phosphatidylserine (PtdSer) exposure, leading to cell lysis. Here, we…

I am running the following (open source) release of RStudio RStudio 2021.09.1+372 “Ghost Orchid” Release (8b9ced188245155642d024aa3630363df611088a, 2021-11-08) for WindowsMozilla/5.0 (Windows NT 10.0; Win64; x64) AppleWebKit/537.36 (KHTML, like Gecko) QtWebEngine/5.12.8 Chrome/69.0.3497.128 Safari/537.36 on Edition Windows 10 Home Version 21H1 Installed on ‎8/‎11/‎2020 OS build 19043.1348 Experience Windows Feature Experience Pack 120.2212.3920.0…

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Abstract The metabolic mechanisms involved in the survival of tumor cells within the hypoxic niche remain unclear. We carried out a synthetic lethal CRISPR screen to identify survival mechanisms governed by the tumor hypoxia–induced pH regulator carbonic anhydrase IX (CAIX). We identified a redox homeostasis network containing the iron-sulfur cluster…