Tag: GENtle

New CRISPR gene-editing system can “drag-and-drop” DNA in bulk

A new technique has been added to the CRISPR gene-editing toolbox. Known as PASTE, the system uses virus enzymes to “drag-and-drop” large sections of DNA into a genome, which could help treat a range of genetic diseases. The CRISPR system originated in bacteria, which used it as a defense mechanism…

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Extra-hematopoietic immunomodulatory role of the guanine-exchange factor DOCK2

Cell isolation, reprogramming and culture Approval was obtained for human cell and tissue sample collection and genetic reprogramming from the Institutional Review Board (protocols 19–252, 18–243, 21–060, 19–284 and 415-E/1776/4-2014, Ethics Committee of the province of Salzburg). Adult samples were collected in accordance with the Declaration of Helsinki after written…

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Breadth of SARS-CoV-2 neutralization and protection induced by a nanoparticle vaccine

Animals and immunizations The study protocol and all veterinarian procedures were approved by the Bioqual IACUC per a memorandum of understanding with the Duke IACUC, and were performed based on standard operating procedures. Macaques studied were housed and maintained in an Association for Assessment and Accreditation of Laboratory Animal Care-accredited…

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Insights on the bacterial composition of Parmigiano Reggiano Pure Whey Starter by a culture-dependent and 16S rRNA metabarcoding portrait

Smid, E. J. et al. Practical implications of the microbial group construction of undefined mesophilic starter cultures. Microb. Cell Factories 13, S2. doi.org/10.1186/1475-2859-13-S1-S2 (2014). Article  Google Scholar  Stadhouders, J. & Leenders, G. J. M. Spontaneously developed mixed-strain cheese starters. Their behaviour in direction of phages and their use within the…

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In vivo electroporation is an answer for drug and gene supply

Electroporation includes utilizing an exterior electrical system to use a prescribed electrical subject to tissue. This transiently opens pores in cell partitions, permitting transport of medication, DNA, and gene therapies. [Photo courtesy of Minnetronix Medical] Why the brand new buzz about an previous method? Harness the potential of an current…

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U.S Bioinformatics Software Market 2022

Bioinformatics Software Market Report: 2022-2027 Bioinformatics Software Market (Newly published report) which covers Market Overview, Future Economic Impact, Competition by Manufacturers, Supply (Production), and Consumption Analysis The market research report on the global Bioinformatics Software industry provides a comprehensive study of the various techniques and materials used in the production…

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autopkgtest regression with openssl 3

I raised the issue to the upstream developer, and he is working on it: github.com/grke/burp/issues/904 He is very responsive and gentle. On Tue, 24 May 2022 20:53:39 +0200 Sebastian Ramacher <sramac…@debian.org> wrote: > Source: burp > Version: 2.4.0-3 > Severity: serious > Tags: sid bookworm > > burp’s autopkgtest fail…

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Turn Your Hamster Into a Killer With This One Simple Step – CRISPR Gene Editing

After carrying out a gene-editing trial on hamsters, a group of neuroscience experts were left “very startled” by the outcome, which showed that the normally placid rodents had been transformed into “aggressive” creatures. According to the press statement, the researchers utilized Syrian hamsters and CRISPR-Cas9, a cutting-edge tech that provides…

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Genome Editing of Human Primary T Cells With Lipid Nanoparticles

By Reka Geczy, PhD, Aruna Balgi, Stella Park, Rita Zhao, Ethan Watt, Maggie Wong, Cooper Webb, Nikita Jain, PhD, Angela Zhang, PhD, Anitha Thomas, PhD, Samuel Clarke, PhD The expression of the chimeric antigen receptor (CAR) on T cells turns a patient’s cells into cell-based cancer therapies and has revolutionized…

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Bioinformatics Software Market Outlook: Post Covid-19 Scenario by 2029

California (United States) – The Bioinformatics Software Market Research Report is a professional asset that provides dynamic and statistical insights into regional and global markets. It includes a comprehensive study of the current scenario to safeguard the trends and prospects of the market. Bioinformatics Software Research reports also track future…

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Endoscopic Ultrasound-Guided Biliary Drainage: Endoscopic Ultrasound-Guided Hepaticogastrostomy in Malignant Biliary Obstruction

Endoscopic Ultrasound-Guided Biliary Drainage (EUS-BD) is an alternative method of biliary decompression in malignant biliary obstruction. Here we describe the technique of EUS guided-Hepaticogastrostomy (EUS-HGS) in a case of unresectable malignant hilar biliary obstruction. Endoscopic Ultrasound-Guided Biliary Drainage:Endoscopic Ultrasound Guided Hepaticogastrostomy in Malignant Biliary Obstruction. Patients with malignant biliary obstruction…

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Real-Time Intravital Multiphoton Microscopy to Visualize Focused Ultrasound and Microbubble Treatments to Increase Blood-Brain Barrier Permeability

This protocol describes the surgical and technical procedures that enable real-time in vivo multiphoton fluorescence imaging of the rodent brain during focused ultrasound and microbubble treatments to increase blood-brain barrier permeability. Ultrasound exposure in the presence of microbubbles has emerged as an effective method to transiently and locally increase the…

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Identification of Hub Genes Associated with COPD Through Integrated Bi

Introduction Chronic obstructive pulmonary disease (COPD) will become the third leading cause of death worldwide.1,2 The incidence of COPD worldwide is 13.1%3 and is 13.7% in the Chinese population over 40 years of age.4 Emphysema is one of the most common phenotypes.1 Over the past few decades, we have conducted…

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In-Nucleus Hi-C in Drosophila Cells

The genome is organized in the nuclear space into different structures that can be revealed through chromosome conformation capture technologies. The in-nucleus Hi-C method provides a genome-wide collection of chromatin interactions in Drosophila cell lines, which generates contact maps that can be explored at megabase resolution at restriction fragment level….

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Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

This protocol uses refractive index detection to measure byproducts of carbon metabolism that are commonly accumulated in lysate-based, cell-free systems. It also makes use of mass spectrometry to detect an even broader panel of central metabolites that are generated in metabolically active lysates. The two techniques used in this protocol…

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Handling Techniques to Reduce Stress in Mice

This paper describes a handling technique in mice, the 3D-handling technique, which facilitates routine handling by reducing anxiety-like behaviors and presents details on two existing related techniques (tunnel and tail handling). My name is Dr.Thomas Prevot. I am a scientist in the Center for Addiction and Mental Health, or CAMH,…

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Serial Block-Face Scanning Electron Microscopy (SBEM) for the Study of Dendritic Spines

Serial Block-Face Scanning Electron Microscopy (SBEM) is applied to image and analyze dendritic spines in the murine hippocampus. Each neuron in the brain is connected with other neuronal cells by approximately 7, 000 synapses. Except that those synapses in the mammalian brain are chiefly located on the small protrusions of…

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TANK prevents IFN-dependent fatal diffuse alveolar hemorrhage by suppressing DNA-cGAS aggregation

Abstract Diffuse alveolar hemorrhage (DAH) is one of the serious complications associated with systemic lupus erythematosus, an autoimmune disease whose pathogenesis involves type I IFNs and cytokines. Here, we show that TANK, a negative regulator of the NF-κB signaling via suppression of TRAF6 ubiquitination, is critical for the amelioration of…

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The Shh/Gli3 gene regulatory network precedes the origin of paired fins and reveals the deep homology between distal fins and digits

Significance In this study, we show that the inactivation of the gli3 gene in medaka fish results in the formation of larger dorsal and paired fins. These mutant fins display multiple radial bones and fin rays which resemble polydactyly in Gli3-deficient mice. Our molecular and genetic analyses indicate that the…

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Building Code Movies With flipbookr

This is a guest post from Gina ReynoldsOpens a new window with contributions from Rachel Goodman, Luca Picci, Conner Surrency, and Brit Woodrum, who provided research assistance while completing their Masters’ at the University of Denver’s Josef Korbel School of International Studies. Gina taught research methodology at University of Denver…

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Improving IVF success: research shines light on embryo health

A team led by the University of Adelaide has developed a new diagnostic tool that has the potential to improve IVF success rates for hopeful parents. One of the greatest challenges for IVF clinics is identifying which embryos are suitable for transfer back into the patient’s uterus. The current gold-standard…

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Analyst Relations Manager Job in , Computer Software Career, $vacancy_job_type Jobs in RStudio

RStudio creates great software that helps people understand data and make better decisions. Our core offering is an open source data science toolchain; as a Public Benefit Corporation, we aim to make it available to everyone, regardless of their economic means. We are looking for an experienced and innovative Analyst…

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Process optimisation and innovative product possibilities through Pulsed Electric Fields

PEF (Pulsed Electric Fields), also known as electroporation, is a technology that is used in food processing to apply short, targeted electrical pulses to plant and animal cells. As a result, the cell membrane changes and the pores are temporarily “opened”. There are various areas of PEF application in the…

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Woolly mammoth extinction blamed on climate change, not human hunting

About 4,000 years ago, the last majestic woolly mammoth roaming Earth vanished, and for decades, scientists believed these colossal ancestors of elephants went extinct because they were relentlessly hunted by humans. DNA analysis of the animals’ old stomping grounds, however, reveals a different story.  © Provided by CNET A trio…

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gmod roleplay model pack

Update: Fixed bumpmaps and rigging errors for all! –I did not make this addon, I’m only supporting the community by adding it to the Gmod 13 workshop.–. Go the the GMOD Textures Page. However, I feel that this pack has both more content at a higher quality than pack 1,…

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Bioconductor Case Studies Use R

Bioconductor Case Studies Use R Analysing time course microarray data using Bioconductor MeV+R: using MeV as a graphical user interface for Omic association studies with R and Bioconductor (eBook Results: We describe RTNsurvival, an R/Bioconductor package that calculates regulon activity profiles…

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Francis Collins Steps Down as NIH Director after 12 Years

Francis S. Collins, MD, PhD, will end the longest tenure of any NIH director—12 years straddling the administrations of three U.S. presidents—when he steps down from the agency’s helm at year’s end. [NIH] Francis S. Collins, MD, PhD, will end the longest tenure of any NIH director—12 years straddling the…

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Unraveling the hidden role of a uORF-encoded peptide as a kinase inhibitor of PKCs

Short open reading frames (ORF) upstream of the canonical ORF (uORFs) are found in about 40% of human protein-coding transcripts (1⇓–3). uORF-containing genes were shown to participate in heavily regulated processes, including differentiation, cell cycle control, and stress responses (4⇓–6). Ribosome profiling data revealed that many uORFs are translated (3,…

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Gene pulser bio rad manual

Gene pulser bio rad manual Bio-Rad Gene Pulser MXcell™ Electroporation System An efficient method for markerless mutant generation by Leishmania donovani: proteasome-mediated down-regulation Visit us on the Web at discover.bio-rad.com Bio-Plex,Leading Life Science Discovery Advance your understanding of cell biology and…

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Nigerian Man Narrates How His Brother Died A Week After Winning Multi-billion Naira Contract In US`

The Chief Executive Officer and the Founder of RETINA-AI Health, Mr. Stephen Odaibo has narrated how his brother, David Gbodi Odaibo who had just won a $125 million contract from the Department of Homeland Security, United States of America, died of cardiac arrhythmia/heart attack in his sleep. Stephen, who took…

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Gromacs 4.5.4 manual

Gromacs 4.5.4 manual OntheStabilityofNegativelyChargedPlatelets inCalcium g_energy(1) [debian man page] Gromacs User Manual Version 4.6The defence of Königsberg had cost the lives of 42,000 German soldiers and 25,000 civilians. He yanked the seaman out and shouted at him to report straight to…

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An epigenetic basis of inbreeding depression in maize

INTRODUCTION Charles R. Darwin documented inbreeding depression as growth disadvantages from self-fertilization compared to outcrossing in many plants (1). Prevailing hypotheses suggest that inbreeding depression results from the exposure of deleterious recessive alleles and/or loss of overdominant alleles due to increased homozygosity (2, 3) or reduced recombination frequency in some…

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