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Tag: guideRNA
Evidence of an intracellular creatine-sensing mechanism that modulates creatine biosynthesis via AGAT expression in human HAP1 cells
Cell lines and reagents HAP1 cells used to generate the reporter cells were grown from a frozen stock of HAP1 (C859) passage 6 purchased from Horizon Discovery LTD (Cambridge, UK). Iscove’s media (Ref: 098–150, Wisent) supplemented with 10% Fetal Bovine serum (Multicell) was used to grow HAP1 cells. All cells…
Computational analysis of Cas proteins unlocks new potential in HIV-1 targeted gene therapy
1Department of Microbiology and Immunology, College of Medicine, Drexel University, United States 2Institute for Molecular Medicine and Infectious Disease, College of Medicine, Drexel University, United States 3Sidney Kimmel Cancer Center, United States The final, formatted version of the article will be published…
Solved Place the following events in the order they occur by
Transcribed image text: Place the following events in the order they occur by listing their assigned letters in that order. When formatting your answer, there should be no spaces or characters in between e.g. ABCDEF A. Cas 9 cuts both strands to generate a double-strand break. B. Cas9 unwinds dsDNA….
A versatile regulatory toolkit of arabinose-inducible artificial transcription factors for Enterobacteriaceae
Design of arabinose-inducible artificial transcription factors A core objective of our work was to develop inducible, heterologous regulators capable of genetically reprogramming gene regulatory networks in Enterobacteriaceae, specifically Salmonella and E. coli. To achieve this, we designed arabinose-inducible ATFs incorporating diverse DBDs originating from CRISPR/dCas9 and plant heterologous TFs (Fig. 1)….
Precision RNA base editing with engineered and endogenous effectors
Gray, M. W. Evolutionary origin of RNA editing. Biochemistry 51, 5235–5242 (2012). Article CAS PubMed Google Scholar Covello, P. & Gray, M. On the evolution of RNA editing. Trends Genet. 9, 265–268 (1993). Article CAS PubMed Google Scholar Yablonovitch, A. L., Deng, P., Jacobson, D. & Li, J. B. The…
Orthogonal Validation: A Means To Strengthen Gene Editing and Gene Modulation Research
From RNA interference (RNAi) to CRISPR, there are several methods that researchers can use to manipulate gene function, each with its own strengths and weaknesses. Orthogonal validation – the synergistic use of different methods – makes genetic perturbation studies more robust. Utilizing complementary methods, including RNAi and CRISPR-knockout, -interference and…
Can I pool samples treated with different sgRNAs when testing for KO effects in RNA-seq analysis?
I have RNAseq data for the following conditions (2 replicates for each): DMSO-treated (control guide), Inhibitor-treated (control guide), DMSO + KO (two different sgRNAs), Treat. + KO (two different guides). I have analyzed the data so far using DESeq and using this kind of grouping (by treatment and sgRNA): metaData…
Genome Editing Market Mainly Driven by Advancements in CRISPR Technology, says Market.us
Genome editing can also be called gene editing. This is a grouping of technologies that allows researchers to alter the DNA of an organism. These technologies enable the addition, deletion or modification of genetic material at certain locations within the genome. Several approaches have been created for genome editing. According…