Tag: guideRNA

Solved Place the following events in the order they occur by

Transcribed image text: Place the following events in the order they occur by listing their assigned letters in that order. When formatting your answer, there should be no spaces or characters in between e.g. ABCDEF A. Cas 9 cuts both strands to generate a double-strand break. B. Cas9 unwinds dsDNA….

Continue Reading Solved Place the following events in the order they occur by

A versatile regulatory toolkit of arabinose-inducible artificial transcription factors for Enterobacteriaceae

Design of arabinose-inducible artificial transcription factors A core objective of our work was to develop inducible, heterologous regulators capable of genetically reprogramming gene regulatory networks in Enterobacteriaceae, specifically Salmonella and E. coli. To achieve this, we designed arabinose-inducible ATFs incorporating diverse DBDs originating from CRISPR/dCas9 and plant heterologous TFs (Fig. 1)….

Continue Reading A versatile regulatory toolkit of arabinose-inducible artificial transcription factors for Enterobacteriaceae

Precision RNA base editing with engineered and endogenous effectors

Gray, M. W. Evolutionary origin of RNA editing. Biochemistry 51, 5235–5242 (2012). Article  CAS  PubMed  Google Scholar  Covello, P. & Gray, M. On the evolution of RNA editing. Trends Genet. 9, 265–268 (1993). Article  CAS  PubMed  Google Scholar  Yablonovitch, A. L., Deng, P., Jacobson, D. & Li, J. B. The…

Continue Reading Precision RNA base editing with engineered and endogenous effectors

Orthogonal Validation: A Means To Strengthen Gene Editing and Gene Modulation Research

From RNA interference (RNAi) to CRISPR, there are several methods that researchers can use to manipulate gene function, each with its own strengths and weaknesses. Orthogonal validation – the synergistic use of different methods – makes genetic perturbation studies more robust. Utilizing complementary methods, including RNAi and CRISPR-knockout, -interference and…

Continue Reading Orthogonal Validation: A Means To Strengthen Gene Editing and Gene Modulation Research

Can I pool samples treated with different sgRNAs when testing for KO effects in RNA-seq analysis?

I have RNAseq data for the following conditions (2 replicates for each): DMSO-treated (control guide), Inhibitor-treated (control guide), DMSO + KO (two different sgRNAs), Treat. + KO (two different guides). I have analyzed the data so far using DESeq and using this kind of grouping (by treatment and sgRNA): metaData…

Continue Reading Can I pool samples treated with different sgRNAs when testing for KO effects in RNA-seq analysis?

Genome Editing Market Mainly Driven by Advancements in CRISPR Technology, says Market.us

Genome editing can also be called gene editing. This is a grouping of technologies that allows researchers to alter the DNA of an organism. These technologies enable the addition, deletion or modification of genetic material at certain locations within the genome. Several approaches have been created for genome editing.  According…

Continue Reading Genome Editing Market Mainly Driven by Advancements in CRISPR Technology, says Market.us