Tag: HDR

New Samsung leak reveals significant Galaxy S24 Ultra photo quality upgrade

Samsung is reportedly adding HDR photo support to its flagship smartphones while posting on Instagram , [+] And Facebook. AFP via Getty Images According to a new report, Samsung Galaxy S24 Ultra owners will enjoy much higher photo quality on the social media platform. December 16 update below: Article originally…

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Bioinformatics-based analysis of the relationship between disulfidptosis and prognosis and treatment response in pancreatic cancer

Identification of DRGs in PCa Figure 1a showed the flow chart of this study. To explore the role of DRGs in PCa, we analyzed the gene expression profiles of these 15 DRGs in PCa patients. As shown in Fig. 1b, for ACTN4, TLN1, IQGAP1, CD2AP, FLNA, MYH9, MYL6, and ACTB genes, the…

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Human RAD52 stimulates the RAD51-mediated homology search

Introduction Homologous recombination (HR) is an evolutionarily conserved process that plays a pivotal role in genome stability, diversity, and plasticity. HR is indeed a key repair pathway able to faithfully repair DNA damages including double-strand breaks (DSBs) and DNA gaps by copying the error-free information from the template DNA normally…

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Matthew Porteus Launches Kamau with Positive Nula-Cell Patient Zero Results

By Jonathan D. Grinstein, PhD What a difference a year makes. Almost 12 months ago, the hopes of Stanford University physician-scientist Matthew Porteus, MD, PhD, to implement a genome editing therapy for sickle cell disease (SCD) seemed all but lost. On January 5, 2023, the company he co-founded, Graphite Bio,…

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Kamau Therapeutics Emerges from Stealth With Clinical-Stage Novel Gene Correction Technology Targeting Life-Threatening Genetic Diseases | DNA RNA and Cells

Kamau Therapeutics Emerges from Stealth With Clinical-Stage Novel Gene Correction Technology Targeting Life-Threatening Genetic Diseases Details Category: DNA RNA and Cells Published on Saturday, 09 December 2023 13:02 Hits: 162 Clinical data on lead program, nula-cel, demonstrating curative outcome in first treated patient with sickle cell disease (SCD) to…

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Map of disease-causing mutations in neurodevelopmental disorders and cancer revealed

Experimental design overview. a Two independent sgRNAs and associated HDR variant libraries are designed at the 5′ and 3′ end of each exon. b The sgRNA, together with the HDR template library are transfected into LIG4-KO Cas9-expressing HAP1 cells. HDR utilizes the library as a template for repair of the…

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Noncoding mutations cause super-enhancer retargeting resulting in protein synthesis dysregulation during B cell lymphoma progression

B cells undergo a series of programmed genomic alterations that enable the immunoglobulin light and heavy chain loci to generate high-affinity antibodies against invading pathogens. First, B cells undergo variability, diversity and joining (VDJ) recombination in the bone marrow with subsequent somatic hypermutation (SHM) and class switch recombination (CSR) occurring…

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Prime editing-mediated correction of the CFTR W1282X mutation in iPSCs and derived airway epithelial cells

Abstract A major unmet need in the cystic fibrosis (CF) therapeutic landscape is the lack of effective treatments for nonsense CFTR mutations, which affect approximately 10% of CF patients. Correction of nonsense CFTR mutations via genomic editing represents a promising therapeutic approach. In this study, we tested whether prime editing,…

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CRISPR Gene Editing: Cas9 and Beyond

Scientists use the CRISPR-Cas9 system to target a DNA sequence of interest near a protospacer-adjacent motif (PAM). The PAM initiates Cas9-DNA binding, a guide RNA (yellow) invades the double helix (blue) and hybridizes with the target DNA, and Cas9 (red) breaks the unwound double-stranded target DNA. iStock Meletios Verras Stay…

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CRISPR and Cas Genes Market scrutinized in the new analysis

CRISPR and Cas Genes Market Size, Share & Trends Analysis Report by Product Type (Vector-based Cas and DNA-free Cas), by Service Type (Cell Line Engineering, gRNA design, Microbial Gene Editing and DNA Synthesis), by Application (Genome Engineering, Disease Models, Functional Genomics and Other) and by End Users (Biotechnology Companies &…

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Vitamin D regulation of gut health

About this project Harnessing vitamin D to promote gut health  If you are keen to advance your career in biomedicine and passionate about conducting innovative gut health research, the University of South Australia – Australia’s University of Enterprise – is offering a hands-on project-based PhD within the Health and Biomedical…

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I made an error when using metawrap to binning

I made an error when using metawrap to binning 1 my code metawrap binning -o bin_out -t 24 -m 200 -a all_contig/all_merge.fasta –metabat2 –maxbin2 –concoct all_fastq/*fastq Error reported as follows sorting the SRR10492802 alignment file [bam_sort_core] merging from 24 files and 24 in-memory blocks… [E::sam_hdr_sanitise] Malformed SAM header at line…

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CRISPR-Cas9 Gene Editing Is On The Cusp Of Something Big

Natali_Mis Gene editing, also known as genome editing, is a method where the DNA of an organism is modified using biotechnological techniques. It allows scientists to add, remove, or alter genetic material at particular locations in the genome. This 2-part series will cover the basics of CRISPR-Cas9 (see below) in…

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What Is CRISPR Technology | CitizenSide

History of CRISPR Technology The history of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) technology traces back to the early 1990s when scientists first noticed repetitive DNA sequences in the genomes of certain bacteria. However, it wasn’t until 2005 that the true potential of CRISPR was realized. It was in…

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A single pseudouridine on rRNA regulates ribosome structure and function in the mammalian parasite Trypanosoma brucei

Cell growth and transfections Procyclic form (PCF) T. brucei, strain 29-1354, which carries integrated genes for the T7 polymerase and the tetracycline repressor, was grown in SDM-79 medium supplemented with 10% fetal calf serum, in the presence of 50 μg/ml hygromycin. Cells were grown in the presence of 15 μg/ml G418 for…

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Integration of xeno-free single-cell cloning in CRISPR-mediated DNA editing of human iPSCs improves homogeneity and methodological efficiency of cellular disease modeling

. 2023 Nov 8:S2213-6711(23)00415-0. doi: 10.1016/j.stemcr.2023.10.013. Online ahead of print. Affiliations Expand Affiliations 1 Turner Institute for Brain and Mental Health, School of Psychological Sciences, Monash University, Melbourne, VIC, Australia. 2 Department of Anatomy & Developmental Biology, Monash University, Melbourne, VIC 3800, Australia; Development and Stem Cells Program, Monash Biomedicine…

Continue Reading Integration of xeno-free single-cell cloning in CRISPR-mediated DNA editing of human iPSCs improves homogeneity and methodological efficiency of cellular disease modeling

Lung and liver editing by lipid nanoparticle delivery of a stable CRISPR-Cas9 RNP

Abstract Lipid nanoparticle (LNP) delivery of CRISPR ribonucleoproteins (RNPs) has the potential to enable high-efficiency in vivo genome editing with low toxicity and an easily manufactured technology, if RNP efficacy can be maintained during LNP production. In this study, we engineered a thermostable Cas9 from Geobacillus stearothermophilus (GeoCas9) using directed…

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IJMS | Free Full-Text | CRISPR/Cas9-Mediated Genome Editing in Cancer Therapy

1. Introduction Recently, the morbidity and mortality rates of cancer have been increasing rapidly, posing a significant threat to human health. Cancer, a refractory and multifaceted disease, fundamentally originates from a cumulative series of mutations in the cellular genome and epigenome. These mutations activate oncogenes and deactivate tumor suppressors, leading…

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Researchers identify key factors involved in DNA repair mechanism

Researchers from Tokyo Metropolitan University have identified key factors in the mechanism behind DNA repair in our bodies. For the first time, they showed that the “proofreading” portion of the DNA replicating enzyme polymerase epsilon ensured safe termination of replication at damaged portions of the DNA strand, ultimately saving DNA…

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IJMS | Free Full-Text | CRISPR/Cas9 Directed Reprogramming of iPSC for Accelerated Motor Neuron Differentiation Leads to Dysregulation of Neuronal Fate Patterning and Function

1. Introduction As the world population ages, neurodegenerative diseases are increasing. The outcome of these diagnoses varies but can lead to fatality 50% of the time within 15–20 months [1]. Treatments for neurodegeneration, in particular motor neuron (MN) diseases, are restricted due to the limitations of motor neuron repair and…

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CRISPR Results Interpretation Conditions of the E.

Transcribed image text: CRISPR Results Interpretation Conditions of the E. coli cells in each starter plate Questions that will help you predict the experiment results: 3. Will non-transformed bacterial cells grow on the KIX/SPT plates? Yes (No (circle the answer) 4. Where do the bacterial cells obtain the donor template…

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Solved CRISPR Results Interpretation Conditions of the E.

Transcribed image text: CRISPR Results Interpretation Conditions of the E. coll cells in each starter plate Questions that will help you predict the experiment results: 3. Will non-transformed bacterial cells grow on the KIX/SPT plates? Yes/No (circle the answer) 4. Where do the bacterial cells obtain the donor template DNA…

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140releng-armv7-default][misc/py-pytorch] Failed for py39-pytorch-2.0.1 in configure

You are receiving this mail as a port that you maintain is failing to build on the FreeBSD package build server. Please investigate the failure and submit a PR to fix build. Maintainer: y…@freebsd.org Log URL: pkg-status.freebsd.org/ampere3/data/140releng-armv7-default/08943441f26e/logs/py39-pytorch-2.0.1.log Build URL: pkg-status.freebsd.org/ampere3/build.html?mastername=140releng-armv7-default&build=08943441f26e Log: =>> Building misc/py-pytorch build started at Thu Nov 2…

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CRISPR/Cas9 bioluminescence-based assay for monitoring CFTR trafficking to the plasma membrane

Introduction Cystic fibrosis (CF) is a lethal, autosomal recessive disorder caused by mutations in the CFTR gene that results in aberrant transport of chloride, bicarbonate, and sodium ions (Kerem et al, 1989; Riordan et al, 1989; Rommens et al, 1989; Tabcharani et al, 1991; Poulsen et al, 1994; Stutts et…

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CRISPR screening in hematology research: from bulk to single-cell level | Journal of Hematology & Oncology

Transcriptomics CROP-seq [46] Jurkat Poly-A CRISPRko Cas9 119 – 5798 (+ 1320 cells with NT gRNA) Lentiviral DROP-seq RNA Perturb-seq [43, 44] K562 Barcode CRISPRi dCas9-KRAB UPR epistasis screen: 9 triplet combinations UPR Perturb-seq experiment: 91 Up to 3 gRNAs in a single vector UPR epistasis screen: 15006 UPR Perturb-seq experiment:…

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A Discussion with Dr. Wenning Qin

In this interview, we delve deep into the pioneering world of xenotransplantation and the evolving landscape of gene editing, with Dr. Wenning Qin, SVP, Innovation & Process Development at eGenesis. Drawing from her vast experience, Dr. Qin shares insights into the challenges, opportunities, and future of xenotransplantation. Please introduce yourself…

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Apple unveils new MacBook Pro featuring M3 chips

October 30, 2023 PRESS RELEASE Apple unveils the new MacBook Pro featuring the M3 family of chips, making the world’s best pro laptop even better 14-inch MacBook Pro with M3 now starts at $1,599 14- and 16-inch models with M3 Pro and M3 Max are available in a gorgeous new space black…

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CRISPR and Cas Genes Market Share, Trends, Future Outlook,

Global CRISPR and Cas genes market is anticipated to grow at a CAGR of 13.5% during the forecast period (2023-2030). The technological advancements in CRISPR & Cas gene systems have contemporarily revolutionized the area of cancer research and therapeutics. As the prevalence of genetic diseases and complex medical conditions continues…

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FastP – Smooth Extreme 60 Fps HDR+ GFX Tool for Android

2.97 880 reviews 100,000+Downloads Free Winner Winner Chicken Dinner With Extreme FPS – Smooth Extreme – HDR 60 Fps We currently don’t have an APK download for this app About FastP – Smooth Extreme 60 Fps HDR+ GFX Tool FastP – Smooth Extreme 60 Fps HDR+ GFX Tool is a…

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Novel replacement strategy holds promise for the treatment of various genetic disorders

Severe Combined Immunodeficiencies (SCIDs) are a group of debilitating primary immunodeficiency disorders, primarily caused by genetic mutations that disrupt T-cell development. SCID can also affect B-cell and natural killer cell function and counts. Left untreated, SCID proves fatal within the first year of life. The conventional treatment for SCID patients…

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Precise gene editing for new therapies

Newswise — Severe Combined Immunodeficiencies (SCIDs) are a group of debilitating primary immunodeficiency disorders, primarily caused by genetic mutations that disrupt T-cell development. SCID can also affect B-cell and natural killer cell function and counts. Left untreated, SCID proves fatal within the first year of life. The conventional treatment for…

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CRISPR’s Innovative “Find-and-Replace” Genome Editing

Bar-Ilan University researchers advance gene therapy for genetic disorders like SCIDs using a refined CRISPR-Cas9 technique, named GE x HDR 2.0. Severe Combined Immunodeficiencies (SCIDs) are a group of debilitating primary immunodeficiency disorders, primarily caused by genetic mutations that disrupt T-cell development. SCID can also affect B-cell and natural killer…

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Find-and-Replace Genome Editing with CRISPR: A Promising Therapeutic Strategy

Newswise — Severe Combined Immunodeficiencies (SCIDs) are a group of debilitating primary immunodeficiency disorders, primarily caused by genetic mutations that disrupt T-cell development. SCID can also affect B-cell and natural killer cell function and counts. Left untreated, SCID proves fatal within the first year of life. The conventional treatment for…

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Precision BioSciences Presents Preclinical Data Highlighting Capabilities of ARCUS for Gene Insertion and Excision at the European Society of Gene & Cell Therapy (ESGCT) 30th Annual Congress

– ARCUS showed durable, high-efficiency gene insertion capabilities in NHPs and comparison to CRISPR-Cas9 – Data demonstrates ability of ARCUS to achieve large gene excisions enabling significant functional muscle improvement in a DMD mouse model DURHAM, N.C., October 26, 2023–(BUSINESS WIRE)–Precision BioSciences, Inc. (Nasdaq: DTIL), an advanced gene editing company…

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What is the main advantage of induced pluripotent stem cells iPSCs in

a) Gene therapy targets somatic cells, while knockout models target germline cells. b) Gene therapy targets germline cells, while knockout models target somatic cells. c) Gene therapy uses NHEJ, while knockout models use HDR. d) Gene therapy targets embryos, while knockout models target adult organisms. **Yamanaka Factors and Regenerative Medicine:**…

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main-powerpc64le-default][misc/pytorch] Failed for pytorch-1.13.1_1 in build

You are receiving this mail as a port that you maintain is failing to build on the FreeBSD package build server. Please investigate the failure and submit a PR to fix build. Maintainer: y…@freebsd.org Log URL: pkg-status.freebsd.org/foul2/data/main-powerpc64le-default/pbc0e38d0f08e_s0afcac3e37/logs/pytorch-1.13.1_1.log Build URL: pkg-status.freebsd.org/foul2/build.html?mastername=main-powerpc64le-default&build=pbc0e38d0f08e_s0afcac3e37 Log: =>> Building misc/pytorch build started at Tue Oct 24…

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Research Fellow – Bioinformatics Job in , Education Career, Full Time Jobs in Curtin University

Job Reference: 929133 About Us The Centre for Crop and Disease Management (CCDM) at Curtin University brings together expertise in plant pathology with biometry, bioinformatics, data science, geographical information systems, farming systems, agribusiness and actuarial science. We have strong industry and academic partnerships and focus on research to overcome…

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Solved Which of the following processes would result in a

Transcribed image text: Which of the following processes would result in a mutation in the DNA upon repair of a double strand break? SSDR NHE HDR CRISPR Question 5 1 pts Inclusion of a template during CRISPR editing can lead to which of the following? an indel Always leads to…

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main-armv7-default][biology/viennarna] Failed for viennarna-2.6.3 in build

You are receiving this mail as a port that you maintain is failing to build on the FreeBSD package build server. Please investigate the failure and submit a PR to fix build. Maintainer: y…@freebsd.org Log URL: pkg-status.freebsd.org/ampere2/data/main-armv7-default/p08943441f26e_s6e92fc9309/logs/viennarna-2.6.3.log Build URL: pkg-status.freebsd.org/ampere2/build.html?mastername=main-armv7-default&build=p08943441f26e_s6e92fc9309 Log: =>> Building biology/viennarna build started at Mon Oct 16…

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Explained: What is CRISPR gene editing and how does it work?

CRISPR-Cas9, often simply referred to as CRISPR, is a revolutionary gene-editing tool that has transformed the field of genetics. In this explainer, we’ll unravel the mysteries of CRISPR gene editing and explore how it works, its applications, and its ethical considerations.The CRISPR RevolutionBefore we dive into the mechanics of CRISPR,…

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Precise genome editing human stem cells for functional interrogation of genetic variation

Abstract Human induced pluripotent stem cells (iPSCs) provide great potential as a tool for basic biological discovery, disease modeling, and ultimately for cell based regenerative medicine but the full realization of these stem cells requires an ability to precisely edit their genome in an efficient way. The relative efficiencies of…

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Innovative strategies of reprograming immune system cells

Introduction Cancer is well characterized by genomic instability resulting in structural alterations that build up with tumor progression.1,2 A subpopulation of undifferentiated cancer cells, known as cancer stem cells (CSCs) are found within the tumor bulk. These cells are responsible for cancer initiation, recurrence and show therapeutic resistance and these…

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IJMS | Free Full-Text | CRISPR-Cas9 Direct Fusions for Improved Genome Editing via Enhanced Homologous Recombination

Over the past decade, CRISPR-Cas9 has found widespread application in loss-of-function mutations, but precise genetic engineering for gene correction or gene replacement therapies has lagged behind. In vivo correction using CRISPR-Cas9 to replace genetic mutations by HR is highly challenging, and very few studies have managed to achieve this [31,32]….

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ENHANCED EFFICIENCY OF RNA-GUIDED CAS12a VERSUS CAS9 TRANSGENE KNOCK-IN AND ACTIVITY AT A SCHISTOSOMA MANSONI GENOME SAFE HARBOR

Abstract Recently, we reported programmed Cas9 mediated insertion of a reporter gene into a gene safe harbor site; GSH1, of Schistosoma mansoni via homology-directed repair (HDR) using overlapping guide RNAs. Here, we report efficient and precise CRISPR/Cas12a-mediated homology-directed insertion of a 5′-C6-PEG10-modified double-stranded transgene bearing microhomology arms, 50nt length at…

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132releng-armv7-quarterly][misc/pytorch] Failed for pytorch-1.13.1_1 in configure

You are receiving this mail as a port that you maintain is failing to build on the FreeBSD package build server. Please investigate the failure and submit a PR to fix build. Maintainer: y…@freebsd.org Log URL: pkg-status.freebsd.org/ampere1/data/132releng-armv7-quarterly/2be22e0743b5/logs/pytorch-1.13.1_1.log Build URL: pkg-status.freebsd.org/ampere1/build.html?mastername=132releng-armv7-quarterly&build=2be22e0743b5 Log: =>> Building misc/pytorch build started at Mon Sep 25…

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main-arm64-default][biology/viennarna] Failed for viennarna-2.6.3 in build

You are receiving this mail as a port that you maintain is failing to build on the FreeBSD package build server. Please investigate the failure and submit a PR to fix build. Maintainer: y…@freebsd.org Log URL: pkg-status.freebsd.org/ampere2/data/main-arm64-default/p85ccf094713a_sc584bb9cac1/logs/viennarna-2.6.3.log Build URL: pkg-status.freebsd.org/ampere2/build.html?mastername=main-arm64-default&build=p85ccf094713a_sc584bb9cac1 Log: =>> Building biology/viennarna build started at Sat Sep 23…

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High-quality single-cell transcriptomics from ovarian histological sections during folliculogenesis

Introduction Single-cell RNA sequencing (RNA-seq) was first achieved by using a quantitative cDNA amplification method and applied to mouse oocytes (Kurimoto et al, 2006; Tang et al, 2009). It has since provided unprecedented opportunities for the study of cellular differentiations, states, and diseases in various biological fields, including developmental biology,…

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Drug delivery systems for CRISPR-based genome editors

Li, Y., Glass, Z., Huang, M., Chen, Z. Y. & Xu, Q. Ex vivo cell-based CRISPR/Cas9 genome editing for therapeutic applications. Biomaterials 234, 119711 (2020). Article  CAS  PubMed  PubMed Central  Google Scholar  Dong, W. & Kantor, B. Lentiviral vectors for delivery of gene-editing systems based on CRISPR/Cas: current state and…

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Single-cell brain organoid screening identifies developmental defects in autism

Stem cell and cerebral organoid culture conditions Feeder-free hES cells or iPS cells were cultured on hES cell-qualified Matrigel (Corning, catalogue no. 354277)-coated plates with Essential8 stem cell medium supplemented with bovine serum albumin (BSA). H9 embryonic stem cells were obtained from WiCell. Cells were maintained in a 5% CO2 incubator at 37 °C….

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Syntrophic entanglements for propionate and acetate oxidation under thermophilic and high-ammonia conditions

Reactor performance revealed temporal changes in propionate degradation rate The four propionate- and acetate-fed reactors used in the study produced biogas with an average methane content of 62–70% (Table S2). The pH was 8.1–8.3, resulting in an ammonia-nitrogen level of 0.7–0.9 g NH3 L-1. This free ammonia level is well above…

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Gene Editing Tools Based on CRISPR/Cas System and Their Improvement Strategies

     Gene Editing Tools Based on CRISPR/Cas System and Their Improvement Strategies XIN Yuan1,TIAN Kai-ren3,QIAO Jian-jun1,2,3,CAIYIN Qing-ge-le1,2,**() 1 School of…

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main-armv7-default][misc/pytorch] Failed for pytorch-1.13.1_1 in configure

You are receiving this mail as a port that you maintain is failing to build on the FreeBSD package build server. Please investigate the failure and submit a PR to fix build. Maintainer: y…@freebsd.org Log URL: pkg-status.freebsd.org/ampere2/data/main-armv7-default/pd101ab5189f9_sb231322dbe/logs/pytorch-1.13.1_1.log Build URL: pkg-status.freebsd.org/ampere2/build.html?mastername=main-armv7-default&build=pd101ab5189f9_sb231322dbe Log: =>> Building misc/pytorch build started at Mon Aug 21…

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Cas9 RNP electroporation (suspension and adherent cells)

You will need the following materials: 1. Purified Cas9-NLS protein, 40 µM in 1x Cas9 buffer 2. Purified sgRNA from in vitro transcription, >48 µM or synthetic 3. Single-stranded DNA HDR donor, 100 µM (as an IDT Ultramer) (optional) 4. Lonza 4D Nucleofector with X Unit 5. Lonza kit: electroporation…

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GenScript Biotech and T-MAXIMUM Biotech Form Strategic Alliance on cGMP sgRNA

GenScript’s cGMP sgRNA reagent services enabled FDA-certified orphan drug designation (ODD) for T-MAXIMUM Biotech’s MT207 CAR-T cell therapy PISCATAWAY, N.J. and NANJING, China, Aug. 9, 2023 /PRNewswire/ — GenScript Biotech, the world’s leading life-science research tools and services provider, and T-MAXIMUM Biotech, a biotechnology company pioneering universal cell therapies, have…

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Next-Generation Sequencing in CRISPR Gene Editing Assessment and Quality Control

The advent of CRISPR-Cas9 gene editing technology has undeniably revolutionized the landscape of genetic research and therapeutic applications. Its ability to precisely modify specific regions of the genome has opened unprecedented opportunities for treating genetic disorders and driving advancements in biotechnology. However, harnessing the full potential of this revolutionary tool…

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Senior Research Fellow, Ophthalmology job with UNIVERSITY OF WESTERN AUSTRALIA

Lions Eye Institute (Centre for Ophthalmology and Visual Science) Position will be located at the Lions Eye Institute (LEI) in Nedlands. LEI is a leading eye research institute in the Asia-Pacific region. 2 full-time positions available on a 5-year fixed term basis Level C, Base salary range:…

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Can genome editing transform ocular disease treatment?

In a recent review published in Experimental & Molecular Medicine, researchers reviewed existing data on the discovery and development of gene-editing technologies and their applications in managing ocular diseases. Study: Genome editing in the treatment of ocular diseases. Image Credit: GarnaZarina/Shutterstock.com Background Gene therapy advances offer a new therapeutic approach for various…

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SOHM Inc. Signs LOI to Acquire Stem Cell Disruptive Technology and Patents

PRESS RELEASE Published August 1, 2023 CHINO HILLS, CA/ ACCESSWIRE/ / August 1, 2023 / SOHM, Inc. (OTC PINK:SHMN), a generic pharmaceutical, nutraceutical and cosmeceutical company that manufactures and markets generic drugs covering numerous treatment categories, announces today that the company has signed a letter of intent to acquire stem…

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main-amd64-default][misc/py-pytorch] Failed for py39-pytorch-2.0.0_2 in configure

You are receiving this mail as a port that you maintain is failing to build on the FreeBSD package build server. Please investigate the failure and submit a PR to fix build. Maintainer: y…@freebsd.org Log URL: pkg-status.freebsd.org/beefy18/data/main-amd64-default/p04fdfe963096_sc620788150/logs/py39-pytorch-2.0.0_2.log Build URL: pkg-status.freebsd.org/beefy18/build.html?mastername=main-amd64-default&build=p04fdfe963096_sc620788150 Log: =>> Building misc/py-pytorch build started at Sat Jul 29…

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CRISPR Market is Expected to Reach $7.1 Billion | MarketsandMarkets.

/EIN News/ — Chicago, July 28, 2023 (GLOBE NEWSWIRE) — In the near future, the CRISPR industry is poised for transformative breakthroughs that will revolutionize medicine, agriculture, and biotechnology. With continuous advancements in CRISPR gene-editing technology, its application will become more widespread and accessible, unlocking unprecedented opportunities for precision medicine…

Continue Reading CRISPR Market is Expected to Reach $7.1 Billion | MarketsandMarkets.

124amd64-quarterly][misc/pytorch] Failed for pytorch-1.13.1_1 in build

You are receiving this mail as a port that you maintain is failing to build on the FreeBSD package build server. Please investigate the failure and submit a PR to fix build. Maintainer: y…@freebsd.org Log URL: pkg-status.freebsd.org/beefy2/data/124amd64-quarterly/1c331580481c/logs/pytorch-1.13.1_1.log Build URL: pkg-status.freebsd.org/beefy2/build.html?mastername=124amd64-quarterly&build=1c331580481c Log: =>> Building misc/pytorch build started at Thu Jul 27…

Continue Reading 124amd64-quarterly][misc/pytorch] Failed for pytorch-1.13.1_1 in build

Stem Cell Manufacturing Market worth $21.8 Billion

CHICAGO, July 26, 2023 /PRNewswire/ — In the near future, the stem cell manufacturing industry is poised to experience unprecedented growth and innovation, revolutionizing healthcare and regenerative medicine. Rapid advancements in biotechnology, automation, and personalized medicine will play pivotal roles in transforming stem cell production and application. The advent of…

Continue Reading Stem Cell Manufacturing Market worth $21.8 Billion

New method brings increased efficiency, precision and reliability in DNA editing

Brain organoid morphology after editing of NOVA1 to the Neandertal state. a, Standard genome editing of NOVA1 as described in51. Independent biological replicates were performed (n = 3) and error bars show the s.e.m. For HDR, replicates are depicted by dots. The right panel shows the percentage of single cell-derived colonies that…

Continue Reading New method brings increased efficiency, precision and reliability in DNA editing

main-arm64-default][biology/mothur] Failed for mothur-1.48.0 in build

You are receiving this mail as a port that you maintain is failing to build on the FreeBSD package build server. Please investigate the failure and submit a PR to fix build. Maintainer: y…@freebsd.org Log URL: pkg-status.freebsd.org/ampere2/data/main-arm64-default/p8fb94260154e_s510fd83138/logs/mothur-1.48.0.log Build URL: pkg-status.freebsd.org/ampere2/build.html?mastername=main-arm64-default&build=p8fb94260154e_s510fd83138 Log: =>> Building biology/mothur build started at Mon Jul 17…

Continue Reading main-arm64-default][biology/mothur] Failed for mothur-1.48.0 in build

cGMP CRISPR sgRNA and Non-viral DNA Payload Manufacturing Capacity Doubled

 GenScript Biotech Corporation, the world’s leading provider of life-science research tools and services, has expanded its cGMP manufacturing capacity for sgRNA and non-viral DNA payloads with a new, state-of-the-art facility in Zhenjiang, Jiangsu, China. This latest expansion provides biopharma companies and gene and cell therapeutic discovery startups a fast-turnaround source…

Continue Reading cGMP CRISPR sgRNA and Non-viral DNA Payload Manufacturing Capacity Doubled

CRISPR-Cas Gene Editing Teaching Resources | Bio-Rad / Free Group Study Rooms with Timer & Music

One of the most exciting recent developments in genetic engineering is CRISPR-Cas9 (CRISPR). CRISPR derives its name from “clustered regularly interspaced short palindromic repeats,” genomic sequences that microbiological used to defending themselves vs viral attacks. Along with CRISPR associated (Cas) bio, bacteria use the sequences toward recognize and disarm future…

Continue Reading CRISPR-Cas Gene Editing Teaching Resources | Bio-Rad / Free Group Study Rooms with Timer & Music

Targeting miRNA by CRISPR/Cas in cancer: advantages and challenges | Military Medical Research

Ishino Y, Shinagawa H, Makino K, Amemura M, Nakata A. Nucleotide sequence of the iap gene, responsible for alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product. J Bacteriol. 1987;169(12):5429–33. Article  CAS  PubMed  PubMed Central  Google Scholar  Bhaya D, Davison M, Barrangou R. CRISPR-Cas systems in…

Continue Reading Targeting miRNA by CRISPR/Cas in cancer: advantages and challenges | Military Medical Research

main-arm64-default][biology/vcftools] Failed for vcftools-0.1.16 in build

You are receiving this mail as a port that you maintain is failing to build on the FreeBSD package build server. Please investigate the failure and submit a PR to fix build. Maintainer: j…@freebsd.org Log URL: pkg-status.freebsd.org/ampere2/data/main-arm64-default/p8fb94260154e_s510fd83138/logs/vcftools-0.1.16.log Build URL: pkg-status.freebsd.org/ampere2/build.html?mastername=main-arm64-default&build=p8fb94260154e_s510fd83138 Log: =>> Building biology/vcftools build started at Sat Jul 15…

Continue Reading main-arm64-default][biology/vcftools] Failed for vcftools-0.1.16 in build

main-amd64-default][biology/viennarna] Failed for viennarna-2.5.1 in build

You are receiving this mail as a port that you maintain is failing to build on the FreeBSD package build server. Please investigate the failure and submit a PR to fix build. Maintainer: y…@freebsd.org Log URL: pkg-status.freebsd.org/beefy18/data/main-amd64-default/p8fb94260154e_s510fd83138/logs/viennarna-2.5.1.log Build URL: pkg-status.freebsd.org/beefy18/build.html?mastername=main-amd64-default&build=p8fb94260154e_s510fd83138 Log: =>> Building biology/viennarna build started at Sat Jul 15…

Continue Reading main-amd64-default][biology/viennarna] Failed for viennarna-2.5.1 in build

Generation of CRB1 RP Patient-Derived iPSCs and a CRISPR/Cas9-Mediated Homology-Directed Repair Strategy for the CRB1 c.2480G>T Mutation

doi: 10.1007/978-3-031-27681-1_83. Affiliations Expand Affiliations 1 Department of Biomedical Engineering, Columbia University, New York, NY, USA. 2 Edward S. Harkness Eye Institute, Department of Ophthalmology, Columbia University Irving Medical Center/New York-Presbyterian Hospital, New York, NY, USA. 3 Jonas Children’s Vision Care, and Bernard & Shirlee Brown Glaucoma Laboratory, Department of…

Continue Reading Generation of CRB1 RP Patient-Derived iPSCs and a CRISPR/Cas9-Mediated Homology-Directed Repair Strategy for the CRB1 c.2480G>T Mutation

main-arm64-default][biology/clustalw] Failed for clustalw-2.1_2 in build

You are receiving this mail as a port that you maintain is failing to build on the FreeBSD package build server. Please investigate the failure and submit a PR to fix build. Maintainer: j…@freebsd.org Log URL: pkg-status.freebsd.org/ampere2/data/main-arm64-default/p8fb94260154e_s510fd83138/logs/clustalw-2.1_2.log Build URL: pkg-status.freebsd.org/ampere2/build.html?mastername=main-arm64-default&build=p8fb94260154e_s510fd83138 Log: =>> Building biology/clustalw build started at Fri Jul 14…

Continue Reading main-arm64-default][biology/clustalw] Failed for clustalw-2.1_2 in build

main-amd64-default][science/lammps] Failed for lammps-2022.06.23.1_7 in build

You are receiving this mail as a port that you maintain is failing to build on the FreeBSD package build server. Please investigate the failure and submit a PR to fix build. Maintainer: y…@freebsd.org Log URL: pkg-status.freebsd.org/beefy18/data/main-amd64-default/p8fb94260154e_s510fd83138/logs/lammps-2022.06.23.1_7.log Build URL: pkg-status.freebsd.org/beefy18/build.html?mastername=main-amd64-default&build=p8fb94260154e_s510fd83138 Log: =>> Building science/lammps build started at Fri Jul 14…

Continue Reading main-amd64-default][science/lammps] Failed for lammps-2022.06.23.1_7 in build

CVC Appeals PTAB Decision in CRISPR Interference | McDonnell Boehnen Hulbert & Berghoff LLP

The decision by the Patent Trial and Appeal Board (PTAB) in favor of Senior Party the Broad Institute, Harvard University, and MIT (collectively, “Broad”) and against Junior Party the University of California/Berkeley, the University of Vienna, and Emmanuelle Charpentier (collectively, “CVC”) sixteen months ago in the latest CRISPR interference No….

Continue Reading CVC Appeals PTAB Decision in CRISPR Interference | McDonnell Boehnen Hulbert & Berghoff LLP

Double-stranded DNA donor with novel structure boosts gene knock-in efficiency

Schematic of odsDNA production and 3 methods of gene knock-in. Credit: Prof. Liang’s group A research group led by Prof. Liang Haojun and researcher Bao Jianqiang from the University of Science and Technology of China (USTC) proposed a novel functional double-stranded DNA (dsDNA) donor that greatly enhances the efficiency of…

Continue Reading Double-stranded DNA donor with novel structure boosts gene knock-in efficiency

131releng-armv7-quarterly][misc/pytorch] Failed for pytorch-1.13.1 in configure

You are receiving this mail as a port that you maintain is failing to build on the FreeBSD package build server. Please investigate the failure and submit a PR to fix build. Maintainer: y…@freebsd.org Log URL: pkg-status.freebsd.org/ampere1/data/131releng-armv7-quarterly/bee14067723b/logs/pytorch-1.13.1.log Build URL: pkg-status.freebsd.org/ampere1/build.html?mastername=131releng-armv7-quarterly&build=bee14067723b Log: =>> Building misc/pytorch build started at Wed Jul 12…

Continue Reading 131releng-armv7-quarterly][misc/pytorch] Failed for pytorch-1.13.1 in configure

131releng-armv7-quarterly][biology/htslib] Failed for htslib-1.17 in build

You are receiving this mail as a port that you maintain is failing to build on the FreeBSD package build server. Please investigate the failure and submit a PR to fix build. Maintainer: j…@freebsd.org Log URL: pkg-status.freebsd.org/ampere1/data/131releng-armv7-quarterly/bee14067723b/logs/htslib-1.17.log Build URL: pkg-status.freebsd.org/ampere1/build.html?mastername=131releng-armv7-quarterly&build=bee14067723b Log: =>> Building biology/htslib build started at Mon Jul 10…

Continue Reading 131releng-armv7-quarterly][biology/htslib] Failed for htslib-1.17 in build

131releng-armv7-default][misc/py-pytorch] Failed for py39-pytorch-2.0.0_2 in configure

You are receiving this mail as a port that you maintain is failing to build on the FreeBSD package build server. Please investigate the failure and submit a PR to fix build. Maintainer: y…@freebsd.org Log URL: pkg-status.freebsd.org/ampere3/data/131releng-armv7-default/12211587418a/logs/py39-pytorch-2.0.0_2.log Build URL: pkg-status.freebsd.org/ampere3/build.html?mastername=131releng-armv7-default&build=12211587418a Log: =>> Building misc/py-pytorch build started at Fri Jul 7…

Continue Reading 131releng-armv7-default][misc/py-pytorch] Failed for py39-pytorch-2.0.0_2 in configure

GenScript Doubles cGMP sgRNA and Non-Viral DNA Payload Manufacturing Capacity

“As a researcher in the gene and cell therapy field, I have experienced firsthand the challenges of finding GMP-grade materials for editing cells. In our lab, we have been leveraging non-viral CRISPR and payload materials to efficiently reprogram various types of immune cells, due to multiple advantages non-viral materials possess,…

Continue Reading GenScript Doubles cGMP sgRNA and Non-Viral DNA Payload Manufacturing Capacity

Genscript Biotech Corporation Doubles cGMP sgRNA and Non-Viral DNA Payload Manufacturing Capacity

GenScript Biotech Corporation has expanded its cGMP manufacturing capacity for sgRNA and non-viral DNA payloads with a new, state-of-the-art facility in Zhenjiang, Jiangsu, China. This latest expansion provides biopharma companies and gene and cell therapeutic discovery startups a fast-turnaround source of the quality components needed to advance novel therapies from…

Continue Reading Genscript Biotech Corporation Doubles cGMP sgRNA and Non-Viral DNA Payload Manufacturing Capacity

06.07.2023 | Press Release | GenScript doubles production capacity for cGMP sgRNA and non-viral DNA payload

Additional 400,000 square feet at GenScript’s Zhenjiang cGMP facility, China provides biopharmaceutical companies and gene/cell therapy research startups with fast access to synthetic CRISPR sgRNA and non-viral single and double-stranded DNA payloads PISCATAWAY, NJ, July 6, 2023 /PRNewswire/ — GenScript Biotech Corporation, the global leader in life science research tools…

Continue Reading 06.07.2023 | Press Release | GenScript doubles production capacity for cGMP sgRNA and non-viral DNA payload

“A Unwinder should return gdb.UnwindInfo instance.” error with NVIDIA Nsight Visual Studio Code Edition – Nsight Visual Studio Code Edition

I added the logFile option to the launch and am including the relevant log information. [17:27:06.813 UTC] GDB result: 11 done,InfoCudaDevicesTable={nr_rows=”8″,nr_cols=”11″,hdr=[{width=”1″,alignment=”1″,col_name=”current”,colhdr=” “},{width=”3″,alignment=”1″,col_name=”device”,colhdr=”Dev”},{width=”14″,alignment=”1″,col_name=”pci_bus”,colhdr=”PCI Bus/Dev ID”},{width=”21″,alignment=”1″,col_name=”name”,colhdr=”Name”},{width=”11″,alignment=”1″,col_name=”description”,colhdr=”Description”},{width=”7″,alignment=”1″,col_name=”sm_type”,colhdr=”SM Type”},{width=”3″,alignment=”1″,col_name=”num_sms”,colhdr=”SMs”},{width=”8″,alignment=”1″,col_name=”num_warps”,colhdr=”Warps/SM”},{width=”10″,alignment=”1″,col_name=”num_lanes”,colhdr=”Lanes/Warp”},{width=”13″,alignment=”1″,col_name=”num_regs”,colhdr=”Max Regs/Lane”},{width=”66″,alignment=”1″,col_name=”active_sms_mask”,colhdr=”Active SMs Mask”}],body=[InfoCudaDevicesRow={current=” “,device=”0″,pci_bus=”06:00.0″,name=”NVIDIA A100-SXM4-80GB”,description=”GA100GL-A”,sm_type=”sm_80″,num_sms=”108″,num_warps=”64″,num_lanes=”32″,num_regs=”256″,active_sms_mask=”0x000000000000000 [17:27:06.813 UTC] GDB -cont-: 11 0000000000000000000000000000000000000000000000000″},InfoCudaDevicesRow={current=” “,device=”1″,pci_bus=”07:00.0″,name=”NVIDIA A100-SXM4-80GB”,description=”GA100GL-A”,sm_type=”sm_80″,num_sms=”108″,num_warps=”64″,num_lanes=”32″,num_regs=”256″,active_sms_mask=”0x0000000000000000000000000000000000000000000000000000000000000000″},InfoCudaDevicesRow={current=” “,device=”2″,pci_bus=”08:00.0″,name=”NVIDIA A100-SXM4-80GB”,description=”GA100GL-A”,sm_type=”sm_80″,num_sms=”108″,num_warps=”64″,num_lanes=”32″,num_regs=”256″,active_sms_mask=”0x0000000000000000000000000000000000000000000000000000000000000000″},InfoCudaDevicesRow={current=” “,device=”3″,pci_bus=”09:00.0″,name=”NVIDIA A100-SXM4-80GB”,description=”GA100GL-A”,sm_type=”sm_80″,num_sms=”108″,num_warps=”64″,num_lanes=”32″,num_regs=”256″,active_sms_mask=”0x0000000000000000000000000000000000000000000000000000000000000000″},InfoCudaDevicesRow={current=” “,device=”4″,pci_bus=”0a:00.0″,name=”NVIDIA A100-SXM4-80GB”,description=”GA100GL-A”,sm_type=” [17:27:06.813 UTC] GDB -cont-: 11 sm_80″,num_sms=”108″,num_warps=”64″,num_lanes=”32″,num_regs=”256″,active_sms_mask=”0x0000000000000000000000000000000000000000000000000000000000000000″},InfoCudaDevicesRow={current=”…

Continue Reading “A Unwinder should return gdb.UnwindInfo instance.” error with NVIDIA Nsight Visual Studio Code Edition – Nsight Visual Studio Code Edition

main-amd64-default][biology/clustalw] Failed for clustalw-2.1_2 in build

You are receiving this mail as a port that you maintain is failing to build on the FreeBSD package build server. Please investigate the failure and submit a PR to fix build. Maintainer: j…@freebsd.org Log URL: pkg-status.freebsd.org/beefy18/data/main-amd64-default/p12211587418a_see8b0c436d/logs/clustalw-2.1_2.log Build URL: pkg-status.freebsd.org/beefy18/build.html?mastername=main-amd64-default&build=p12211587418a_see8b0c436d Log: =>> Building biology/clustalw build started at Tue Jul 4…

Continue Reading main-amd64-default][biology/clustalw] Failed for clustalw-2.1_2 in build

Custom CRISPR sgRNA Lentiviral Vectors & Viruses

Use abm’s All-in-One lentivectors and lentiviruses that express spCas9 or FnCas12a (FnCpf1) along with the sgRNA to simplify the delivery of CRISPR/Cas9 technology right into the target cell. Alternatively, custom sgRNA lentivectors and lentiviruses can be used to deliver sgRNAs into cells that already express the spCas9 nuclease, and thus…

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Cambridge urges action to reduce the carbon footprint of computing. –

Cambridge scientists have laid out principles for making computational science more sustainable. This field is responsible for many discoveries, from understanding human genomes to developing treatments against cancer. Researchers from the Department of Public Health and Primary Care of the University of Cambridge, writing in Nature Computational Science, argue that…

Continue Reading Cambridge urges action to reduce the carbon footprint of computing. –

Cas9 Proteins | Applied Biological Materials Inc.

CRISPR is the most versatile technology for genome editing and abm offers the largest selection of Cas9 proteins for CRISPR experiments. Cas9 Ribonucleoproteins (RNPs) are Cas9 proteins in complex with in vitro transcribed sgRNA. These RNPs can be used for in vitro pre-screening sgRNA candidates and can be directly transfected…

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eQTL and sQTL from 16 cattle tissues (linear mixed model).

Gene expression and RNA splicing explain large proportions of the heritability for complex traits in cattle” (www.biorxiv.org/content/10.1101/2022.05.30.494093v1) Summary stats of cis and trans eQTL and sQTL mapped from 16 cattle tissues (N~5000) using linear mixed model. Multi-tissue eQTL and sQTL (from meta-analysis) are also included. For details, please read “esQTLSumdataXiang2022/README.txt”…

Continue Reading eQTL and sQTL from 16 cattle tissues (linear mixed model).

Award-winning compact laser projector serves up 100-inch HD visuals

Optoma has launched what is described as the world’s most compact portable RGB triple laser projector, which comes in standard and short-throw variants capable of throwing 100-inch Full HD visuals up on a wall or screen. The ML1080 DLP projector has a native 1080p resolution with a throw ratio of…

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Encountering an error in sample2markers.py -i SRS013951.sam.bz2 -o pkl_files/ – StrainPhlAn

I am attempting to utilize Strainphlan by referring to the documentation. In Step 1, it should generate a sam.bz2 and bowtie2out.bz2 file, and indeed they are generated. However, upon proceeding to Step 2, an error regarding duplicate sequences is encountered. Interestingly, if I download the provided sam.bz2 file from the…

Continue Reading Encountering an error in sample2markers.py -i SRS013951.sam.bz2 -o pkl_files/ – StrainPhlAn

Fast, precise and cloning-free knock-in of reporter sequences in vivo with high efficiency

. 2023 Jun 13;dev.201323. doi: 10.1242/dev.201323. Online ahead of print. Affiliations Expand Affiliation 1 Centre for Discovery Brain Sciences, University of Edinburgh, EH16 4SB, UK. Item in Clipboard Yiran Zhang et al. Development. 2023. Show details Display options Display options Format AbstractPubMedPMID . 2023 Jun 13;dev.201323. doi: 10.1242/dev.201323. Online ahead of…

Continue Reading Fast, precise and cloning-free knock-in of reporter sequences in vivo with high efficiency

What’s the correct way to store bam record in a vector and free them?

What’s the correct way to store bam record in a vector and free them? 1 Here’s my demo code #include <stdio.h> #include “htslib/sam.h” #include <vector> #include <fstream> #include <string> #include <iostream> int main(int argc, char *argv[]) { samFile *fp = sam_open(argv[1], “r”); hts_idx_t *idx = sam_index_load(fp, argv[1]); bam_hdr_t *h =…

Continue Reading What’s the correct way to store bam record in a vector and free them?

Reading same BAM file twice with htslib

I would like to read iteratively the same BAM file twice using htslib. My option is to use hts_open, sam_hdr_read, then sam_read1 as much as needed to read the file until EOF. Is there a possibility to “rewind”, and use sam_read1 again from the beginning of the file, without closing…

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Activation-inducible CAR expression enables precise control over engineered CAR T cell function

Primary cells PBMCs were either isolated from fresh buffy coats via density gradient centrifugation using Biocoll (Biochrom) or selected from a Leukapheresis material using in house T cell selection technology ATC. Buffy coats were obtained from autologous adult female or male blood donors at the Institute for Anesthesiology, German heart…

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Reduction of biofilm formation of Escherichia coli by targeting quorum sensing and adhesion genes using the CRISPR/Cas9-HDR approach, and its clinical application on urinary catheter.

Escherichia coli is a common cause of biofilm-associated urinary tract infections (UTIs). Biofilm formation in E. coli is responsible for various indwelling medical device-associated infections, including catheter-associated urinary tract infections (CAUTIs). This study aimed to reduce biofilm formation of E. coli ATCC 25922 by knocking out genes involved in quorum…

Continue Reading Reduction of biofilm formation of Escherichia coli by targeting quorum sensing and adhesion genes using the CRISPR/Cas9-HDR approach, and its clinical application on urinary catheter.

ssDNA is not superior to dsDNA as long HDR donors for CRISPR-mediated endogenous gene tagging in human diploid RPE1 and HCT116 cells | BMC Genomics

An optimized CRISPR knock-in method using long dsDNA donors for efficient tagging with fluorescent proteins in human diploid cells. Given the widespread use of CRISPR-mediated generation of knock-in cell lines, it is informative to compare the performance of long dsDNA and ssDNA donors in a simple and practical knock-in method….

Continue Reading ssDNA is not superior to dsDNA as long HDR donors for CRISPR-mediated endogenous gene tagging in human diploid RPE1 and HCT116 cells | BMC Genomics

Gates Center Stem Cell Biobank and Disease Modeling Core

Stem Cell Biobank and Disease Modeling Core Overview   The Stem Cell Biobank and Disease Modeling Core (SCB&DM core) offers complete services related to the production of high quality human induced pluripotent stem cells (iPSCs) from patient-derived somatic cells and genetic engineering of iPSCs.  The core employs a patent-pending, RNA-based methodology to…

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USTC has made new progress in the optimization of gene knock-in donors

Professor Liang Haojun, Hefei National Research Center for Physical Sciences at the Microscale, School of Chemistry and Materials Science, University of Science and Technology of China, cooperated with researcher Bao Qiangqiang from the Department of Life Sciences and Medicine, and optimized and proposed the current gene knock-in donors in terms…

Continue Reading USTC has made new progress in the optimization of gene knock-in donors

Site-specific gene knock-in and bacterial phytase gene expression in Chlamydomonas reinhardtii via Cas9 RNP-mediated HDR

Introduction Microalgae encompass a large number of organisms with both prokaryotic and eukaryotic nature, making them an archetypal platform for recombinant technology (Malla et al., 2021). The nutraceutical significance of microalgae is characterized by their rich natural biomolecules and high amount of protein, vitamins, and lipid substances (Potvin and Zhang, 2010;…

Continue Reading Site-specific gene knock-in and bacterial phytase gene expression in Chlamydomonas reinhardtii via Cas9 RNP-mediated HDR