Tag: HEK293

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection

Current therapeutic innovations, such as CAR-T cell therapy, are heavily reliant on viral-mediated gene delivery. Although efficient, this technique is accompanied by high manufacturing costs, which has brought about an interest in using alternative methods for gene delivery. Electroporation is an electro-physical, non-viral approach for the intracellular delivery of genes…

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A cell-based phenotypic library selection and screening approach for the de novo discovery of novel functional chimeric antigen receptors

Cell lines and culture Unless stated otherwise, all tumor target cell lines were sourced from the ATCC via LGC Standards. Human endogenous mesothelin (MSLN)-positive target cell lines H-226 (lung carcinoma, MSLN+, ATCC® CRL-5826™) and AsPC-1 (ATCC CRL-1682), and MSLN-negative Raji control cells (ATCC CCL-86) were cultured in RPMI-1640 Glutamax (Life Technologies,…

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Recombinant Human CD94/KLRD1 Protein, HEK293 Cells, His Tag, 12649-H07H

CD94/KLRD1 Protein, Human, Recombinant (His Tag): 产品信息 纯度 > 95 % as determined by SDS-PAGE 生物活性 Testing in progress 蛋白构建 A DNA sequence encoding the human KLRD1 (Q13241-1) (Lys32-Ile179) was expressed with a polyhistidine tag at the N-terminus. 表达宿主 HEK293 Cells 分子量 The recombinant human KLRD1 consists of…

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A novel antagonist of TRPM2 and TRPV4 channels: Carvacrol

This article was originally published here Metab Brain Dis. 2022 Jan 6. doi: 10.1007/s11011-021-00887-1. Online ahead of print. ABSTRACT The overload cytosolic free Ca2+ (cCa2+) influx-mediated excessive generation of oxidative stress in the pathophysiological conditions induces neuronal and cellular injury via the activation of cation channels. TRPM2 and TRPV4 channels…

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Addressing the Challenges of AAV Production

Adeno-associated virus (AAV) vectors are a versatile and appealing gene therapy delivery platform, capable of targeting a wide range of cell types. However, AAV must be produced in a living system, and issues with scalability and high production costs have so far limited the widespread adoption of the technology. Technology…

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Sod1 integrates oxygen availability to redox regulate NADPH production and the thiol redoxome

Significance Cu/Zn superoxide dismutase (Sod1) is a key antioxidant enzyme, and its importance is underscored by the fact that its ablation in cell and animal models results in oxidative stress; metabolic defects; and reductions in cell proliferation, viability, and lifespan. Curiously, Sod1 detoxifies superoxide radicals (O2•−) in a manner that…

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Figure 3 | BK Polyomavirus Activates HSF1 Stimulating Human Kidney Hek293 Cell Proliferation

BKPyV induces Hek293 cell proliferation at late time of infection. Hek293 cells were infected with BKPyV for 1 h. (a, b) Cells were counted by Trypan blue exclusion after 24 h. Data are expressed as (; ; ). (c) Cell proliferation was determined through immunofluorescence detection of incorporated BrdU. Data are expressed…

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NPSR1 / NPSR / GPR154 Protein Over-Expression Lysate

Western blot Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate Western blot Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate Western blot Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression…

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Single-cell delineation of lineage and genetic identity in the mouse brain

STICR lentiviral library preparation and validation We synthesized a high-complexity lentivirus barcode library that encodes approximately 60–70 million distinct oligonucleotide RNA sequences (STICR barcodes). STICR barcodes comprised three distinct oligonucleotide fragments cloned sequentially into a multicloning site within the 3′ UTR of an enhanced green fluorescent protein (eGFP) transgene under…

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WT1 regulates HOXB9 gene expression in a bidirectional way

This article was originally published here Biochim Biophys Acta Gene Regul Mech. 2021 Sep 8:194764. doi: 10.1016/j.bbagrm.2021.194764. Online ahead of print. ABSTRACT The homeoboxB9 (HOXB9) gene is necessary for specification of the anterior-posterior body axis during embryonic development and expressed in various types of cancer. Here we show that the…

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CRISPR Applications in Medicine Depend on Minimizing Off-Target Editing

By Rolf Turk, PhD Genome editing technologies, such as CRISPR, are being applied to better understand basic biological systems as well as to research new kinds of gene and cell therapies. The CRISPR-Cas9 system comprises a Cas9 endonuclease protein that forms a complex with a guide RNA (gRNA) molecule, which…

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