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Tag: HEK293
ZsGreen/Luciferase Safe-Harbor HEK293 Cell Line | BPS Bioscience
Product information “ZsGreen/Luciferase Safe-Harbor HEK293 Cell Line” Recombinant stable HEK293 cell line constitutively expressing ZsGreen (bright green fluorescent protein derived from Zoanthus sp. reef coral) and firefly luciferase, which have been stably integrated into the AAVS1 safe harbor locus on chromosome 19 using CRISPR/Cas9. Expression of ZsGreen and the luciferase…
Recombinant Human Serpin E1, Transfected HEK293 Overexpression Cell Culture Supernatant
Recombinant Human Serpin E1, Transfected HEK293 Overexpression Cell Culture Supernatant | RayBiotech The store will not work correctly in the case when cookies are disabled. JavaScript seems to be disabled in your browser. For the best experience on our site, be sure to turn on…
Unusual mode of dimerization of retinitis pigmentosa-associated F220C rhodopsin.
Unusual mode of dimerization of retinitis pigmentosa-associated F220C rhodopsin. Publication , Journal Article Khelashvili, G; Pillai, AN; Lee, J; Pandey, K; Payne, AM; Siegel, Z; Cuendet, MA; Lewis, TR; Arshavsky, VY; Broichhagen, J; Levitz, J; Menon, AK Published in: Sci Rep Mutations in the G protein-coupled receptor (GPCR) rhodopsin are a…
Frontiers Publishing Partnerships | Construction of recombinant adenovirus-5 vector to prevent replication-competent adenovirus occurrence
Introduction In recent years, recombinant adenoviral vectors have been used in different fields of biomedical sciences such as in vitro and in vivo gene transfer, vaccine development, and gene therapy (Russell, 2000; Mitani and Kubo, 2002). Multiple features of recombinant adenoviral vectors such as high packaging capacity for transgene insertion,…
Sequential deregulation of histone marks, chromatin accessibility and gene expression in response to PROTAC-induced degradation of ASH2L
Loss of ASH2L prevents cell proliferation We have studied the molecular and cellular consequences of Ash2l loss in mouse embryo fibroblasts (MEFs) with floxed Ash2l alleles and an inducible Cre-ER recombinase (iMEF-Ash2lfl/fl-Cre-ER). While the knockout of Ash2l was rapid, the downstream effects, including the decrease in promoter-associated H3K4me3, altered gene…
Subtype HEK293 Medium Market Growth Factor, Size, Share, Opportunity, Business Trend, Strategy, By Type, Application, Key Players, Regions, and Demand Forecast to 2031
PRESS RELEASE Published December 14, 2023 Global “Subtype HEK293 Medium Market” Report offers a thorough examination of the worldwide Subtype HEK293 Medium market, encompassing critical insights into key trends, opportunities, recent developments, and challenges. This research report is a compilation of information and analysis obtained from various sources to assist…
Comprehensive Review of Small Interfering RNAs (siRNAs)
Introduction Cancer, also called malignancy, is a group of diseases caused by the rapid multiplication and spread of malignant cells.1,2 According to statistics from the National Cancer Center, more than 100 types of cancer have been identified.3 The ability of malignant tumor cells to metastasize via the blood-lymphatic system and…
Atg7 senses ATP levels and regulates AKT1-PDCD4 phosphorylation-ubiquitination axis to promote survival during metabolic stress
Atg7 interacts with PDCD4 and negatively regulates PDCD4 protein levels Previous studies have demonstrated the role of Atg7 in maintaining metabolic homeostasis by initiating autophagy and arresting the cell cycle17,18,22. Herein, the function of Atg7 was more comprehensively investigated and it was determined whether Atg7 reduces protein synthesis to save…
Regulation of the ER-Resident Mannosidase EDEM2 in HEK293 Cells
Abstract EDEM2 plays an important role as the first enzyme that acts during mannose trimming of N-glycosylated proteins in the ERAD machinery. Although EDEM2 expression has been reported to be transcriptionally regulated by the IRE1-sXBP1 pathway, very little is known about how endogenous EDEM2 protein expression is regulated. In this…
Validation of transfection and expression of protein constructs within HEK293 cells.
To enable an analysis of Nrxn and Nlgn pairing, we coexpressed different Nrxn and Nlgn constructs with specific nanobarcode proteins. This enables us to provide the different Nrxn- and Nlgn-containing cells with a recognizable identity, without having to modify the additional proteins themselves by nanobarcode tagging. However, this implies that…
A naturally occurring polyacetylene isolated from carrots promotes health and delays signatures of aging
Cell culture conditions Unless otherwise stated, cells were cultured with DMEM (Sigma Aldrich; #D6429) and 4.5 g/l glucose, phenol red, 10% FBS, and 1% penicillin/streptomycin with pH 7.4 and were incubated at 37 °C, 5% CO2, and 95% relative humidity. Wild-type HepG2 (#300198), HEK293 (#300192), and MCF-7 (#300273) were derived from CLS…
Protective Effects of Bombyx batryticatus Protein-Rich Extract Against Cisplatin-Induced Nephrotoxicity in HEK293 Cells and a Mouse Model
. 2023 Dec;26(12):927-938. doi: 10.1089/jmf.2023.K.0182. Affiliations Expand Affiliations 1 Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Korea. 2 Department of Biotechnology, College of Life Science and Biotechnology, Korea University, Seoul, Korea. Item in Clipboard Jeong Moo Han et al. J Med Food. 2023 Dec. Show details Display options…
C1 MCK2 is an inactive chemokine.
(A) HEK293 cells were transfected with pFuse vectors expressing human IgG-Fc fusion proteins of wt, C1 and 129stop MCK2. 24 hours after transfection, supernatants from transfected cells were harvested, proteins precipitated with EtOH and detected by WB using an antibody specific for human IgG. Supernatants from mock-transfected cells were used…
Why are HEK293 cells used for transfection?
What is one example of ongoing gene therapy can be used in keratoplasty?5 answersGene therapy in ophthalmology has shown potential for use in keratoplasty. The cornea is an ideal target for gene therapy due to its accessibility, low volume, and special immunological status. Studies have focused on improving gene transfer…
Cell Membrane Chromatography Using HALO-tag Technology
A group of scientists from Xi’an, China have created a new system for analyzing cell membranes based around haloalkane dehalogenase protein tag (HALO)-tag technology. Their research was published in Talanta (1). Cell membrane chromatography (CMC) is effective for studying receptors with multiple transmembrane structures, such as MAS-related G protein-coupled receptor…
Has anyone ever done chip-seq for p300 in hepg2 cells?
What metabolic condition will increase the expression of CD36 in liver?3 answersNon-alcoholic fatty liver disease (NAFLD) and hepatic steatosis are metabolic conditions that increase the expression of CD36 in the liver. In patients with obstructive sleep apnea (OSA), NAFLD was significantly more frequent and severe, and hepatic CD36 expression was…
High-throughput ligand profile characterization in novel cell lines expressing seven heterologous insect olfactory receptors for the detection of volatile plant biomarkers
Generation of the stable OR cell lines In vivo measured ligand profile data derived from the DoOR database (neuro.uni-konstanz.de/DoOR/default.html)19,20 were used to select 12 ORs, which can be appropriate to detect the pathogen-related VOCs relevant for our study. To assemble a novel cell-based olfactory panel, 11 stable cell lines were…
New genetic links to coronary artery calcification uncovered
A recent research letter published in the journal Nature Cardiovascular Research describes two new genetic loci associated with coronary artery calcification (CAC). CAC is a measure of atherosclerosis and predicts coronary artery disease (CAD) events. Coronary calcification is a manifestation of atherosclerotic plaque. It is suggested to contribute to plaque…
Variants in the CETP gene affect levels of HDL cholesterol by reducing the amount, and not the specific lipid transfer activity, of secreted CETP
Fig 1. Lipid transfer activity, secretion, and synthesis of CETP missense variants. HEK293 cells were transiently transfected with the WT-CETP plasmid or the indicated CETP plasmids. (A) The lipid transfer activities in the media of transfected cells were determined. To account for the number of cells in the lysate secreting…
A dual-targeted drug inhibits cardiac ryanodine receptor Ca2+ leak but activates SERCA2a Ca2+ uptake
Introduction During each heartbeat, the intracellular calcium concentration ([Ca2+]i) cycles dynamically between low resting diastolic and high active systolic levels within cardiomyocytes (Bers, 2002; Eisner et al, 2017; Sankaranarayanan et al, 2017): upon electrical excitation, Ca2+ influx via voltage-gated CaV1.2 channels activates Ca2+-induced Ca2+ release via SR ryanodine receptor type…
Test our GMP media for your T and HEK cells
At Sartorius, we offer a wide range of high-quality media and critical raw materials. We help you find the best formulation for your processes by offering expert support for media and services. Our comprehensive services, along with this, can help you quickly commercialize your product. Find out more and request…
HEK293 cells for transfection? | 3 Answers from Research papers
how much genome scale metabolic models are presents in literature? 5 answers What are the factors that influence disaster risk governance 5 answers what creates bitterness when hydrolysing pea protein. Please rank (and rate impact) the variables. 5 answers Is death in the Spanish curriculum? 5 answers Why do nanocrystalline…
how to get heart muscle cells from skin fibroblasts
What are the advantages and disadvantages of using an incubator for cell culture? 3 answers Are there any papers that look at long-term killing in vitro? 4 answers are there any Splicing Changes in the Blood of DM1? 5 answers how does vascularization after muscle injury work? 5 answers What…
DRIP-qPCR validation – Mendeley Data
Description 1. HEK293 cells were obtained from the National Collection of Authenticated Cell Culture of China (accession number: SCSP-5014) and cultured in DMEM supplemented with 10% FBS and penicillin/streptomycin (100U/ml) at 37°C with 5%CO2. Expression vector carrying NOTCH2NLC exon1 with different numbers of GGC repeats were prepared and transfected into…
Building AAV Manufacturing Capacity For Large Patient Diseases
By Tony Hitchcock, AGH Bioconsulting Over the last decade, we have witnessed the development of multiple AAV-based gene therapies offering transformational cures to patients with debilitating and often fatal conditions. We are now seeing some of these products receiving regulatory approval (Table 1).1 However, the exceedingly high prices levied on…
The ERAD pathway also contributes to ROMK mutant turnover in HEK293 cells.
(A) Stability assays of HEK293 cells expressing wild-type ROMK, or ROMK carrying the G228E, L320P, or N377K mutation. HEK293 cells transfected with the indicated expression vector were treated with MG-132 or the equivalent volume of DMSO for 30 min, at which point cycloheximide was added. Cells were next processed as…
Cell surface levels of putative disease-associated ROMK mutants are reduced in HEK293 cells.
(A) A cell-surface biotinylation assay is shown of the relative surface expression levels of the indicated ROMK variants. HEK293 cells expressing wild-type ROMK or the G228E or L320P mutant were treated with biotin, processed, and incubated with streptavidin beads before an immunoblot analysis was performed. 1% input was collected prior…
A Cre-dependent massively parallel reporter assay allows for cell-type specific assessment of the functional effects of non-coding elements in vivo
Animal models All procedures involving animals were approved by the Institutional Animal Care and Use Committee (IACUC) at Washington University in St. Louis, MO. Veterinary care and housing was provided by the veterinarians and veterinary technicians of Washington University School of Medicine under Dougherty lab’s approved IACUC protocol. All protocols…
FAT10 is phosphorylated by IKK{beta} to inhibit the antiviral type-I interferon response
Introduction The innate immune system represents the host´s first-line defense against viral infections (Koyama et al, 2008). In this context, IFN-I are produced by all nucleated cells and represent the principal cytokines that counteract viral replication (Ivashkiv & Donlin, 2014). In fact, several receptors of the infected cell can recognize…
The DNA-binding induced (de)AMPylation activity of a Coxiella burnetii Fic enzyme targets Histone H3
Statistics and reproducibility Anti-AMP IP for LC-MS/MS analysis was performed in three independent biological replicates (n = 3). TSA assay data represents technical triplicates. Time-resolved (de)AMPylation analyzed by LC-MS was performed as biological triplicates. CD measurements were performed in technical triplicates. Anisotropy data is shown as technical triplicates. Anisotropy measurements were repeated…
Reduced steroid activation of elephant shark GR and MR after inserting four amino acids from the DNA-binding domain of lamprey corticoid receptor-1
Fig 3. Concentration-dependent transcriptional activation by corticosteroids and progesterone of FLAG-tagged wild-type and mutated elephant shark MR. Plasmids for FLAG-tagged wild-type elephant shark MR and mutated elephant shark MR (with a TRQG insert) were expressed in HEK293 cells with either an MMTV-luciferase promoter or a TAT3-luciferase promoter. Cells were treated…
Highly oxidized albumin is cleared by liver sinusoidal endothelial cells via the receptors stabilin-1 and -2
List of reagents Chloramine-T trihydrate (Merck, Darmstadt, Germany), Copper(II)Sulphate, Penicillin, Streptomycin, RPMI-1640 (Sigma-Aldrich, Burlington, MA, USA), RPMI-1640 (Euroclone, Pero, Italy), DMEM low glucose (Sigma-Aldrich), Trypsin–EDTA (Sigma-Aldrich), Blasticidin hydrochloride (Sigma-Aldrich), Trichloroacetic acid (Merck), Fetal Bovine Serum (Merck, Darmstadt, Germany), Iodine 125 Radionuclide (Perkin Elmer, Waltham, Mass., USA), Iodogen™ iodination reagent (Pierce,…
Both chimpanzee adenovirus-vectored and DNA vaccines induced long-term immunity against Nipah virus infection
Ethics statement The animal experiments were approved by the Animal Ethics Committee of the Wuhan Institute of Virology, Chinese Academy of Sciences (approval number: WIVA21202104). All animal experiments involving NiV were executed in the animal biosafety level 4 (ABSL-4) facility at the National Biosafety Laboratory (Wuhan), Chinese Academy of Sciences….
Simplified Single-Cell Isolation
Sponsored content brought to you by Many increasingly used applications, such as cell line engineering and sequencing (including ATAC-seq and RNA-seq) along with single-cell genomics, require single-cell isolation as a starting point. But the cost and complexity of cell sorters have traditionally relegated this technology to larger lab groups or…
Glycoengineered keratinocyte library reveals essential functions of specific glycans for all stages of HSV-1 infection
Generation of a glycogene knock out library in HaCaT keratinocytes HaCaT is a human keratinocyte cell line capable of forming a stratified squamous epithelium, and thus allows evaluating the infection of the skin tropic HSV-1 in both cell and organotypic tissue culture. In order to address the role of specific…
protein principles assignment 2.docx – Protein expression and purification hold pivotal roles in diverse scientific fields and industries including
Protein expression and purification hold pivotal roles in diverse scientific fields and industries, including biotechnology and pharmaceuticals. This report provides an in-depth account of my protein expression and purification strategy, with a specific focus on the production of Green Fluorescent Protein (GFP) using Human Embryonic Kidney (HEK293) cells. Additionally, it…
Recombinant Human Retinoblastoma-Like 2 (RBL2), transfected HEK293 cell lysate
Recombinant Human Retinoblastoma-Like 2 (RBL2), transfected HEK293 cell lysate | RayBiotech The store will not work correctly in the case when cookies are disabled. JavaScript seems to be disabled in your browser. For the best experience on our site, be sure to turn on…
Does A315T mutation of TDP43 causes cytoplasmic aggregates in hek293 cells?
The A315T mutation of TDP43 does not cause cytoplasmic aggregates in HEK293 cells. The A315T mutation is one cause of familial amyotrophic lateral sclerosis (ALS) and is associated with cytoplasmic aggregates in neurons of ALS patients. However, the formation of cytoplasmic aggregates in HEK293 cells is not observed in the…
Recombinant Sus scrofa (pig) Cystatin B, Transfected HEK293 Cell Culture Supernatant
Recombinant Sus scrofa (pig) Cystatin B, Transfected HEK293 Cell Culture Supernatant | RayBiotech The store will not work correctly in the case when cookies are disabled. JavaScript seems to be disabled in your browser. For the best experience on our site, be sure to turn on Javascript in your browser….
Why are Hek293 cells predominantly used in production of viral vectors compared to CHO
Hek293 cells are predominantly used in the production of viral vectors compared to CHO cells due to several reasons. Firstly, Hek293 cells have been found to be efficient in transducing different cell types and have been successfully used in gene and cell therapy protocols. Secondly, transcriptomic analysis has revealed that…
Reduction of retinal ganglion cell death in mouse models of familial dysautonomia using AAV-mediated gene therapy and splicing modulators
Animals All mice were housed in the AALAC-accredited Animal Resource Center at Montana State University. All animal use protocols were approved by the Montana State University Institutional Animal Care and Use Committee (IACUC) (protocol No. 2020-15-IA; Bozeman, MT). The study fulfilled the ARRIVE guidelines, and all experiments complied with relevant…
Methods for Scaling and Protein Purification
CHO cells are the predominant host for biotherapeutic protein expression, with roughly 70% of licensed biologics manufactured in CHO. Multiple attributes make CHO cells desirable for bioproduction, including the ability to adapt to high-density suspension culture in serum-free and chemically-defined media and the incorporation of post-translational modifications that are biologically…
[Production and antigenicity analysis of a recombinant insulinoma associated protein-2 in HEK293 cells]
Insulinoma-associated protein-2 (IA-2) is a transmembrane glycoprotein belonging to the tyrosine phosphatase-like protein family as well as an important autoantigen in the diagnosis of type 1 diabetes. IA-2 products have been marketed in Europe and the United States. At present, commercially available IA-2 antigens are either the recombinant IA-2ic domain…
Structure and electromechanical coupling of a voltage-gated Na+/H+ exchanger
Expression and purification of SLC9C1 The gene encoding the S. purpuratus SLC9C1 protein (NP_001091927.1), referred to as SLC9C1, was synthesized (Thermo Fisher Scientific) and subcloned into the pcDNA3.1(+) vector followed by a TEV (Tobacco Etch Virus nuclear-inclusion-a endopeptidase) recognition site, eGFP and a TwinStrep tag at the C terminus. An…
A Bioconductor workflow for processing, evaluating,…
Introduction Proteins are responsible for carrying out a multitude of biological tasks, implementing cellular functionality and determining phenotype. Mass spectrometry (MS)-based expression proteomics allows protein abundance to be quantified and compared between samples. In turn, differential protein abundance can be used to explore how biological systems respond to a perturbation….
Structure of human TRPM8 channel
Reagents and chemicals pFastBac Dual His6 MBP N10 TEV LIC cloning vector (5 C) was purchased from Addgene (Addgene plasmid no. 30123; www.addgene.org/30123). All restriction enzymes, the pFastBac1 vector, and DH10Bac cells were purchased from Thermo Fisher Scientific. Sf9 cells were purchased from ATCC. Q5® High-Fidelity DNA Polymerase, the Monarch® DNA…
Passive diffusion accounts for the majority of intracellular nanovesicle transport
Introduction Trafficking of proteins, lipids, and other molecules between cellular compartments is carried out by vesicular carriers. Material destined for transfer is packaged into a small trafficking vesicle at the donor compartment; the vesicle must then travel to its destination, before fusing with the target compartment to deliver the material…
Solved Fig. 3. GIGVF2/AEHP complex formation is critical for
Transcribed image text: Fig. 3. GIGVF2/AEHP complex formation is critical for repression of 1/nbf mRNA translation and enabling viral replication. (A) ELSA measurement of ifN-1) production in WT, 4EHP.KO, and GIGVF2-KO HEK 93 cels transiently expressing TLR3 folowing 6 h of treatment with 1 pgimL poly(:C. Data are presented KO…
Inactive S. aureus Cas9 downregulates alpha-synuclein and reduces mtDNA damage and oxidative stress levels in human stem cell model of Parkinson’s disease
Cloning of CRISPR/sgRNA lentiviral constructs with fluorescent selection markers A tetracycline-inducible promoter (TRE3G) was used to control the expression of S. aureus dCas9 in a lentiviral vector. To facilitate selection of cells by FACS, pHR:TRE3G-SadCas9-2xKRAB-p2a-tdTomato (Addgene ID #209298) was subcloned from a pHR:TRE3G-SadCas9-2xKRAB-p2a-zeo (A gift from Professor Stanley Qi), where zeocin…
Exosome Technologies Market Size, Share, Segments, Trends And Drivers For 2023-2032
Exosome Technologies Global Market Report 2023 – Market Size, Trends, And Global Forecast 2023-2027 The Business Research Company’s Exosome Technologies Global Market Report 2023 – Market Size, Trends, And Forecast 2023-2027 The global exosome technologies market size is expected to reach $0.14 billion in 2027 at a CAGR of 26.72%”…
High-resolution analysis of bound Ca2+ in neurons and synapses
Introduction Calcium ions (Ca2+) act as important second messengers in all cells, regulating vital aspects of cellular function from cell proliferation to cell death (Schwarz & Blower, 2016; Giorgi et al, 2018). In the nervous system, Ca2+ signaling plays a special role in the regulation of neuronal and synaptic activities….
Direct regulation of the cardiac ryanodine receptor (RyR2) by O-GlcNAcylation | Cardiovascular Diabetology
Human right atrial appendages (RAA) Human RAA were obtained from consenting patients (ethics: LRS/12/01/001/AM17), with or without Type 2 Diabetic Mellitus (T2DM), undergoing on-pump coronary artery bypass graft surgery. Patient characteristics are shown in Table 1. Patients with pre-operative arrhythmia were excluded from the study. Table 1 Patient characteristics Patients were…
Subcellular distribution of the rAAV genome depends on genome structure
Optimization of transfection condition for viral genome staining The transfection was prepared by various concentrations of plasmids expressing DD-dSpyCas9-mCherry-APEX2 (pAPEX2) and sgITR (psgITR) with the transfection agent to stain the rAAV genome inside the cells for TEM. To investigate the target viral genome, a specific staining technique is necessary for…
Genome Engineering Oxford (GEO) Facility
We also are actively involved in developing new gene editing methods and provide opportunities for collaborations on more extensive or challenging uses of CRISPR techniques such as development of new construct designs, application to whole organisms, development and use of pooled genome-wide CRISPR libraries. Services/Equipment available Engineered mammalian and bacterial…
1. After measuring the O.D.260 of the genomic DNA and
Transcribed image text: 1. After measuring the O.D.260 of the genomic DNA and RNA samples that you isolated from HeLa and HEK293 cells in lab, you obtain the following values: a. Assuming you did not dilute your sample before taking these measurements, what are the concentrations of DNA and RNA…
Genome editing of patient-derived iPSCs identifies a deep intronic variant causing aberrant splicing in hemophilia A
. 2023 Oct 4:bloodadvances.2023010838. doi: 10.1182/bloodadvances.2023010838. Online ahead of print. Affiliations Expand Affiliations 1 Jichi Medical University, Tochigi, Japan. 2 Tokyo Medical University, Tokyo, Japan. 3 Jichi Medical University, Simotsuke, Tochigi, Japan. 4 The University of Tokyo, Tokyo, Japan. Item in Clipboard Takafumi Hiramoto et al. Blood Adv. 2023. Show details…
Exosome Technologies Market by Medical Composition, Type, End-Use Industry and Region Analyzed With Trends and Forecasts to 2030
PRESS RELEASE Published October 4, 2023 The Exosome Technologies Market 2023 Global Industry Research Report is a comprehensive business study on the current state of the Exosome Technologies Industry providing competitive analysis in form of 10 company profiles along with their product picture and specification, capacity production price cost production value and…
The effect of oleic acid supplementation on lipid droplet production, betaoxidation and rotavirus replication
Abstract Rotavirus (RV) is the most common cause of severe acute gastroenteritis in infants and young children globally. Rotavirus infections induce cytoplasmic inclusion bodies called viroplasms serving as a site of RV genome replication and assembly. Viroplasms recruit lipid droplets (LDs), which play major roles in energy homeostasis and is…
In planta expression of human polyQ-expanded huntingtin fragment reveals mechanisms to prevent disease-related protein aggregation
Plant material and constructs A. thaliana lines of Columbia-0 (Col-0) ecotype were employed, including WT, toc159 (refs. 58,59) and cct8-2 (ref. 60). Seeds underwent surface sterilization and germination on solid 0.5× Murashige and Skoog (M&S) medium with vitamins, lacking sucrose. Plants were incubated in a growth chamber at 22 °C under…
TRIM25 targets p300 for degradation
Introduction Protein levels are regulated at several nodes. One mode of protein level regulation acts through enhancing or reducing gene transcription. Gene transcription can be stimulated by binding transcription factors to promoters or enhancers of target genes and by posttranslational modifications of transcription factors and histones. Such modifications can be…
A computationally designed antigen eliciting broad humoral responses against SARS-CoV-2 and related sarbecoviruses
Phylogenetic analysis Protein sequences of spike proteins were downloaded from the NCBI virus database for all the known sarbecoviruses (June 2020). A multiple sequence alignment was generated using MUSCLE36. The resulting multiple sequence alignment was pruned to the RBD region, filtered at 95% sequence identity and used as input for…
Upregulation of WDR6 drives hepatic de novo lipogenesis in insulin resistance in mice
WDR6 is upregulated in response to insulin during insulin resistance We created an IR mouse model by feeding animals a HFD for 6 weeks20. Insulin injection was then performed (Fig. 1a). The phosphorylation of AKT, a key readout of the insulin signalling pathway21, showed decreased response to insulin, indicating resistance…
MAST4 promotes primary ciliary resorption through phosphorylation of Tctex-1
Introduction The primary cilium (9 + 0 architecture) is a microtubule-based immotile sensory organelle in vertebrates (Tucker et al, 1979). Enormous progress has been made in the past several decades concerning our understanding of the structure and functions of the primary cilium (Sung & Leroux, 2013). The subdomain between the…
Establishing a Stable Recombinant Protein Production Cell Line Using CRISPR-Cas9 Mediated Genome Editing of HEK293 Cells
Recombinant proteins are effective therapeutics for many diseases and thus their demand has increased over the past few decades. Today, most recombinant proteins are produced in bacteria and non-mammalian cells hosts such as Chinese Hamster Ovary (CHO) due to their high production efficiencies. Despite this, issues pertaining to the utilization…
Profiling a ncRNA in Breast Cancer
The project focused on identifying and characterizing novel non-coding RNAs (ncRNAs) and miRNAs that are misregulated in different cancer subtypes, focusing on breast cancer cells. The project aims to explore the regulatory roles of ncRNAs in cancer cells and identify potential therapeutic targets for breast cancer treatment. RNA1 was used…
Avirmax Inc. Will Attend the BioProcess International Meeting in Boston
HAYWARD, Calif., Sept. 13, 2023 /PRNewswire/ — Avirmax Inc., Vice President of Research, Dr. Li Ou, and Associate Director of Operations, Ms. June Song, will attend the BioProcess International meeting in Boston between September 18th to 21st, 2023. As an Associate Sponsor, Avirmax will have a booth (#223) during the exhibit…
Recombinant Human FGFR2 protein(IIIb), His-Avi-tagged FGFR2-055H
Description : Four distinct genes encoding closely related FGF receptors, FGF R1 – 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII…
Development of short hairpin RNA expression vectors targeting the internal ribosomal entry site of the classical swine fever virus genomic RNA | BMC Biotechnology
Design of siRNA targeting CSFV-IRES domain III region The CSFV-IRES-expressing cells were established using the pCAG vector and swine SK-L and human HEK293 (pCI5) cells (Additional file 1: Fig. S1) as previously described [15]. The CSFV-IRES activity was measured as the ratio of firefly luciferase (F-luc) to renilla luciferase (RL)…
CRISPR imaging reveals chromatin fluctuation at the centromere region related to cellular senescence
Plasmid construction For CRISPR imaging, an sgRNA expression vector, pLH-spsgRNA 2 plasmid (Addgene, Watertown, MA, USA), was amplified, and the oligonucleotides targeting α-satellite DNA repeats (sgRNA1-5)33,49 and 5S rDNA34 were inserted into the BbsI site as previously described16. The sgRNA sequences were as follows: sgRNA1: 5′-CTTTTTGTGGAATTTGCAAG-3′ sgRNA2: 5′-GACTTGAAACACTCTTTTTG-3′ sgRNA3: 5′-TGGAGATTTCAGCCGCTTTG-3′…
DOCK8 Full-Length MS Protein Standard
Product Information Protein Name dedicator of cytokinesis 8 Description DOCK8 Full-Length MS Protein Standard (NP_982272), Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine, was produced in human 293 cells (HEK293) with fully chemically defined cell culture medium to obtain incorporation efficiency at Creative-Proteomics. This gene encodes a member of…
New blood test pinpoints Parkinson’s disease through mitochondrial DNA damage
In a recent study published in the journal Science Translational Medicine, researchers investigate the potential of mitochondrial deoxyribonucleic acid (mtDNA) injury as a serological marker for Parkinson’s disease (PD) using the novel Mito DNADX test. Study: A blood-based marker of mitochondrial DNA damage in Parkinson’s disease. Image Credit: Kateryna Kon / Shutterstock.com What…
Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress.
Guo D, Han J, Adam BL, Colburn NH, Wang MH, Dong Z, Eizirik DL, She JX, Wang CY Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress. Biochem Biophys Res Commun. 2005 Dec 2;337(4):1308-18. Epub 2005 Oct 10. The substrates of SUMO4, a novel member for the…
Detecting Host Cell Proteins in AAVs
By Gail Dutton Methods to detect host cell proteins (HCPs) in monoclonal antibodies (mAbs) and similar classes of biotherapeutics are standard and reliable, but not, it seems, in gene therapy viral vector products. Adeno-associated viruses (AAVs) and other advanced biotherapeutic products are more complex than mAbs, with a range of…
Exosome Technologies Global Market Report 2023
New York, Aug. 25, 2023 (GLOBE NEWSWIRE) — Reportlinker.com announces the release of the report “Exosome Technologies Global Market Report 2023” – www.reportlinker.com/p06486472/?utm_source=GNW , Fujifilm Holdings Corporation, Anjarium Biosciences, NOVADIP Biosciences S.A., Exopharm Ltd., Organicell Regenerative, Evox Therapeutics, Direct Biologics, ExoCan Healthcare Technologies Pvt. Ltd., ExoCoBio, ExonanoRNA LLC, ILIAS Biologics…
Addressing regulatory guidance for HEK293 cells & AAV-based therapeutics manufacturing
Cell & Gene Therapy Insights 2023; 9(7), 965–973 10.18609/cgti.2023.123 Michael Brewer Testing and characterization in cell and gene therapy manufacturing is critical for AAV-based therapeutics. Regulatory guidance affecting those using HEK293 cells or AAV-based therapeutics is evolving over time, especially pertaining to quantitating residual host cell…
Significant differences in capsid properties and potency between AAV vectors produced in Sf9 and HEK293 cells
For successful vector-based gene therapy manufacturing, the selected adeno-associated virus (AAV) vector production system must produce vector at sufficient scale. However, concerns have arisen regarding the quality of vector produced using different systems. In this study, we compared AAV serotypes 1, 8, and 9 produced by two different systems (Sf9/baculovirus…
Light-Induced Dielectrophoresis for Characterizing the Electrical Behavior of Human Mesenchymal Stem Cells
Here, we present light-induced dielectrophoresis as a label-free approach for characterizing heterogeneous cell lines, specifically human mesenchymal stem cells (hMSCs). This paper describes a protocol for using and optimizing a microfluidic device with a photoconductive layer to characterize the electrical behavior of hMSCs without altering their native state. This light-induced…
Identification of two novel variants of the DMD gene
Introduction Duchenne muscular dystrophy (DMD, OMIM#310200) is a severe X-linked recessive, inherited neuromuscular disorder, characterized by rapidly progressive muscle weakness and muscle wasting throughout the body.1 DMD is more common in males than females, with an incidence rate of 1:5000 and 1:50,000,000, respectively.2 Female heterozygotes theoretically have 50% normal cells…
CalTech Team Develops Programmable RNA Exporters for Live Mammalian Cells
NEW YORK – A team of researchers from the California Institute of Technology has developed a new nanoparticle transport system that can achieve programmable RNA export from living mammalian cells. Described in a Cell paper published last month, the mechanism, termed controlled output and uptake of RNA for interrogation, expression,…
Direct observation of the conformational states of PIEZO1
No statistical methods were used to predetermine sample size. The experiments were not randomized and investigators were not blinded to allocation during experiments and outcome assessment. Expression constructs The coding sequence of mouse PIEZO1 (E2JF22, UniprotKB entry) was codon-optimized, synthesized and cloned into the pcDNA3.1 plasmid. An amber stop codon…
Wearable Interfaces for Gene Therapies: Medical Delivery Solutions
As technology advances, the integration of electronic devices with biological systems is becoming a frontier in medical innovation. Wearable devices that can monitor, sense, and even administer therapies are no longer a distant dream but a tangible reality. This article explores the fascinating world of electrogenetic interfaces, a cutting-edge technology…
Exosome Technologies Market Segments, Opportunity, Growth
Exosome Technologies Global Market The Business Research Company’s global market reports are now updated with the latest market sizing information for the year 2023 and forecasted to 2032 The Business Research Company’s Exosome Technologies Global Market Report 2023 identifies rising prevalence of chronic diseases as the major driver for the…
Recombinant Rat Epcam protein, hFc-tagged Epcam-7481R
Home / Products / Recombinant Proteins / Recombinant Rat Epcam protein, hFc-tagged Recombinant Rat Epcam protein, hFc-tagged Online Inquiry Epcam Related Products By Species By Source By Tag Price Inquiry Welcome! For price inquiries, please feel free to contact us through the…
Mapping RNA-Seq reads onto viral genome
Mapping RNA-Seq reads onto viral genome 0 Hi everyone, I have 6 files of paired-end 75 nt RNA-Seq reads from HEK293 I want to map onto the AAV genome. I got the reference genome as a fasta file and the annotation file as gff3/gtf from NCBI. For mapping onto the…
Implanted cells triggered by electricity used to drive in vivo gene expression
Design of the direct-current-activated transgene expression switch in mammalian cells. a, Schematic illustration of the stimulation setup for monolayer cultures. Each well of a 24-well plate has two platinum wires that function as anode and cathode, placed 0.6 cm apart submerged in the culture medium. When electric current is applied, bubbles…
CRISPR library screening to develop HEK293-derived cell lines with improved lentiviral vector titers
doi: 10.3389/fgeed.2023.1218328. eCollection 2023. Affiliations Expand Affiliation 1 Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, MD, United States. Free PMC article Item in Clipboard Brian J Iaffaldano et al. Front Genome Ed. 2023. Free PMC article Show details Display…
An electrogenetic interface to program mammalian gene expression by direct current
Key plasmids used in this study Construction details for all vectors are provided in Supplementary Table 1. Key plasmids included (1) a constitutive KEAP1 expression vector (pJH1004, PhCMV-KEAP1-pA) and the corresponding vector containing inverted terminal repeats (ITRs) of Sleeping Beauty (SB) transposase (pJH1054, ITR-PhCMV-KEAP1-P2A-BlastR-pA-ITR) for stable cell line generation; (2)…
A Comprehensive Guide- Nao Medical
Are you interested in exploring the potential of GLP-1 analog transfection in HEK293 cells? Look no further! In this comprehensive guide, we will walk you through everything you need to know about this cutting-edge technique. What is GLP-1 Analog Transfection? GLP-1 analog transfection is a powerful method used to introduce…
Phenotypic and molecular basis of SIX1 variants linked to non-syndromic deafness and atypical branchio-otic syndrome in South Korea
Clinical phenotypes This study included eight affected patients (three males and five females) from five unrelated families, segregating with SIX1 variants (Fig. 1a). A comprehensive overview of their clinical phenotypes, encompassing both major and minor criteria, is provided in Table 1. All eight individuals (100%) experienced sensorineural hearing loss (SNHL), with…
Antiviral HIV-1 SERINC restriction factors disrupt virus membrane asymmetry
Constructs and expression of human SERINC proteins SERINC3 The gene that encodes human SERINC3 (Genscript-OHu02717D) was inserted upstream of a thrombin protease cleavable linker (LVPRGS) and Strep II epitope (WSHPQFEK) in a modified pFastBac vector by In-Fusion cloning (Clontech). Mutagenesis using the QuikChange Site-Directed Mutagenesis kit (Agilent) was performed to…
SORL1 | ALZFORUM
c.-2484C>A Substitution Non-Coding Upstream of 5′ UTR Zhang et al., 2015 c.-1287T>C(SNP 1) Substitution Non-Coding Upstream of 5′ UTR Rogaeva et al., 2007 c.-1204G>T Substitution Non-Coding Upstream of 5′ UTR Zhang et al., 2015 A2G Substitution Substitution | Missense Coding Exon 1 Unknown. Unknown. Holstege et al., 2022 S6T Substitution…
Integrated analysis of copy number variation-associated lncRNAs identifies candidates contributing to the etiologies of congenital kidney anomalies
Study design We aimed to investigate the potential contribution of lncRNAs to the pathogenicity of CAKUT associated CNVs. 19 recurrent CAKUT-associated CNVs were identified based on clinical researches of congenital anomalies of the kidney and urinary tracts (CAKUT) cases3,4,12,13 (Table 1). We retrieved lncRNAs located within these genomic regions as candidate…
Researchers demonstrate targeted epigenome editing in the promoter region of several genes using sgRNA/dCas9 complexes
In a recent study posted to the preprint server Research Square* while under review for publication in Epigenetics & Chromatin, researchers develop and characterize a highly specific EpiEditing system using catalytically inactivated Cas9 (dCas9) to achieve allele-specific deoxyribonucleic acid (DNA) methylation (ASM). Study: Development of super-specific epigenome editing by targeted allele-specific…
The opioid tramadol blocks the cardiac sodium channel Nav1.5 in HEK293 cells
Background and aims: Opioids are associated with increased risk of sudden cardiac death. This may be due to their effects on the cardiac sodium channel (Nav1.5) current. In the present study, we establish whether tramadol, fentanyl, or codeine affect Nav1.5 current. Methods: Using whole-cell patch-clamp methodology, we studied the effects…
How to automate cloning for CRISPR workflows
The process of gene editing involves the transformation of a substantial number of cells, followed by the isolation of individual cells from the larger population to establish clonal colonies. Due to the significant number of gene edits required in modern research and the labor-intensive workflow, there is a pressing…
Morphomigrational description as a new approach connecting cell’s migration with its morphology
Here we present the building blocks of morphomigrational description and provide practical examples of their application in an analysis of migrating cells. First, we show the rationale for choosing those descriptors with explanation how do they complement each other in describing the cell behaviour. Then, we apply the new method to…
Mammalian Cell Transfection – KCell
Transient Transfection Development Customization Process Transient Transfection of Mammalian Cells Mammalian transient expression system is widely used in many fields such as early discovery of antibody protein drugs, in vivo toxicity experiments, diagnostic reagents, and vaccine development due to the advantages of producing more naturally folded and post-translationally modified recombinant…
Mesenchymal stromal cells facilitate resolution of pulmonary fibrosis by miR-29c and miR-129 intercellular transfer
Cell culture hTERT immortalized adipose-derived mesenchymal stem cells (hTERT-MSC, ASC52telo (ATCC® SCRC-4000™)), hTERT-MSCs with Crispr–Cas9 miR-29с knockdown and hTERT-MSCs with Crispr–Cas9 nonsense knockdown (biobank of the Institute for Regenerative Medicine, Lomonosov MSU (human.depo.msu.ru)) were cultured in Advance Stem Cell Basal Medium with Advance Stem Cell Growth Supplement (HyClone). All experiments…
Fluorescence lifetime FRET assay for live-cell high-throughput screening of the cardiac SERCA pump yields multiple classes of small-molecule allosteric modulators
Molecular biology A two-color intramolecular human SERCA2a (2CS) biosensor, based on human cardiac SERCA2a fused to green fluorescent protein (eGFP) and red fluorescent protein (tagRFP) was developed to detect structural changes that are related to the functional changes of SERCA20,22,25. Briefly, tagRFP was genetically fused to the N-terminus of SERCA…
Endogenous tagging using split mNeonGreen in human iPSCs for live imaging studies
Abstract Endogenous tags have become invaluable tools to visualize and study native proteins in live cells. However, generating human cell lines carrying endogenous tags is difficult due to the low efficiency of homology-directed repair. Recently, an engineered split mNeonGreen protein was used to generate a large-scale endogenous tag library in…