Tag: HEK293T

A cell-based phenotypic library selection and screening approach for the de novo discovery of novel functional chimeric antigen receptors

Cell lines and culture Unless stated otherwise, all tumor target cell lines were sourced from the ATCC via LGC Standards. Human endogenous mesothelin (MSLN)-positive target cell lines H-226 (lung carcinoma, MSLN+, ATCC® CRL-5826™) and AsPC-1 (ATCC CRL-1682), and MSLN-negative Raji control cells (ATCC CCL-86) were cultured in RPMI-1640 Glutamax (Life Technologies,…

Continue Reading A cell-based phenotypic library selection and screening approach for the de novo discovery of novel functional chimeric antigen receptors

Lead exposure induces dysregulation of constitutive heterochromatin hallmarks in live cells

This article was originally published here Curr Res Toxicol. 2021 Dec 11;3:100061. doi: 10.1016/j.crtox.2021.12.001. eCollection 2022. ABSTRACT Lead (Pb) is a heavy metal contaminant commonly found in air, soil, and drinking water due to legacy uses. Excretion of ingested Pb can result in extensive kidney damages due to elevated oxidative…

Continue Reading Lead exposure induces dysregulation of constitutive heterochromatin hallmarks in live cells

Twin Prime Editing Promises More Precise DNA Changes Without Double-Strand Breaks

NEW YORK – Researchers at the Broad Institute have developed a new prime editing method that allows for more precise replacement or excision of DNA sequences at endogenous human genomic sites, without the need for double-strand DNA breaks (DSBs). In a paper published on Thursday in Nature Biotechnology, researchers led…

Continue Reading Twin Prime Editing Promises More Precise DNA Changes Without Double-Strand Breaks

Selection of DNA Aptamers Recognizing EpCAM-Positive Prostate Cancer b

Introduction Prostate cancer (PCa) is one of the most common genitourinary system malignant tumor in men worldwide. In Asian countries, many patients with PCa are often diagnosed at an advanced stage maybe mostly because of a large population base with relatively backward economic development and imperfect cancer screening system of…

Continue Reading Selection of DNA Aptamers Recognizing EpCAM-Positive Prostate Cancer b

MYO10 drives genomic instability and inflammation in cancer

INTRODUCTION Genomic instability often refers to the existence of a variety of DNA alterations, ranging from single nucleotide changes (such as base substitution, deletion, and insertion) to chromosomal rearrangements (e.g., gain or loss of a segment or the whole chromosome) (1). Loss of genome stability can lead to early onset…

Continue Reading MYO10 drives genomic instability and inflammation in cancer

Arginine monomethylation by PRMT7 controls MAVS-mediated antiviral innate immunity

Innate immunity is the first line of defense against microbial infection. In response to RNA virus infection, mitochondrial antiviral signaling protein (MAVS) plays an important role in innate antiviral immune responses. Arginine methylation is a Post-translational Modification (PTM) in histone and non-histone proteins that can affect numerous cellular activities. Previous…

Continue Reading Arginine monomethylation by PRMT7 controls MAVS-mediated antiviral innate immunity

High-purity production and precise editing of DNA base editing ribonucleoproteins

Abstract Ribonucleoprotein (RNP) complex–mediated base editing is expected to be greatly beneficial because of its reduced off-target effects compared to plasmid- or viral vector–mediated gene editing, especially in therapeutic applications. However, production of recombinant cytosine base editors (CBEs) or adenine base editors (ABEs) with ample yield and high purity in…

Continue Reading High-purity production and precise editing of DNA base editing ribonucleoproteins