Tag: HepG2

Boyi Yu,1– 4 Xuanyi Lu,5 Xianglong Feng,1– 4 Ting Zhao,1– 4 Jiaxin Li,1– 4 Yudie Lu,5 Fei Ye,1– 4 Xiongxiong Liu,1– 4 Xiaogang Zheng,1– 4 Zheyu Shen,5 Xiaodong Jin,1– 4 Weiqiang Chen,1– 4 Qiang Li1– 4 1Biomedical Center, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, People’s Republic of…

This study demonstrated the novel coactivation mechanism of mRNA and rRNA underlying the role of FIR and its splicing variant, FIRΔexon2. Because FIR is immunoprecipitated with P89 and P62 of TFIIH [7], FIR potentially affects RNAPII-mediated transcription via TFIIH. As expected, FIR affected the mRNA expression of various cancer-related genes…

Cell culture conditions Unless otherwise stated, cells were cultured with DMEM (Sigma Aldrich; #D6429) and 4.5 g/l glucose, phenol red, 10% FBS, and 1% penicillin/streptomycin with pH 7.4 and were incubated at 37 °C, 5% CO2, and 95% relative humidity. Wild-type HepG2 (#300198), HEK293 (#300192), and MCF-7 (#300273) were derived from CLS…

Weighted gene co-expression network analysis GSE131329 has comprehensive clinical data, and WGCNA was carried out with this expression profile. WGCNA is a systems biology method for characterizing gene association patterns between different samples, used to identify highly synergistic sets of genes and to screen for candidate biomarker genes or therapeutic…

What metabolic condition will increase the expression of CD36 in liver?3 answersNon-alcoholic fatty liver disease (NAFLD) and hepatic steatosis are metabolic conditions that increase the expression of CD36 in the liver. In patients with obstructive sleep apnea (OSA), NAFLD was significantly more frequent and severe, and hepatic CD36 expression was…

Full vacancy here: www.universiteitleiden.nl/vacatures/2023/q4/14246-phd-researcher-in-systems-toxicology-connecting-co-expression-networks-to-injury-and-pathology Key responsibilities Systems toxicology is emerging as key methodology to analyze and interpret omics data in the chemical safety: it mimics the modular and multi-factorial nature of diseases and perturbational statuses. In particular, Dr. Callegaro and colleagues in the PI group of Bob van de Water…

DIAMOND mice develop HCC in the context of obesity and insulin resistance To investigate in depth the genome-wide transcriptional and epigenetic changes that occur during development of MAFLD and obesity-related hepatocellular carcinoma (HCC), we performed RNA-sequencing (RNA-seq) and Chromatin ImmunoPrecipitation-sequencing (ChIP-seq) of liver tumours (HCC) and liver control tissue (FL)…

1Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 330008, People’s Republic of China; 2Department of General Surgery, Jiujiang Hospital of Traditional Chinese Medicine, Jiujiang, Jiangxi, 332007, People’s Republic of China; 3Queen Mary School, Nanchang University, Nanchang, Jiangxi, 330038, People’s Republic of China Correspondence: Hongliang Luo,…

Domcke, S. et al. Evaluating cell lines as tumour models by comparison of genomic profiles. Nat. Commun. 4, 2126 (2013). Article  ADS  PubMed  Google Scholar  Köbel, M. et al. Ovarian carcinoma subtypes are different diseases: Implications for biomarker studies. PLoS Med. 5(12), e232 (2008). Article  PubMed  PubMed Central  Google Scholar …

Long terminal repeats are enriched within active enhancers identified by STARR-seq. a Schematic representation of the analysis pipeline. b Upset plot for overlap analysis of STARR-seq peaks with TEs in GP5d and HepG2 cells with the number of peaks in each category indicated; total number of peaks 15,390 and 11,951…

In a recent article published in Nature Medicine, researchers pursued evidence of whether the interaction between the patatin-like phospholipase domain-containing 3 (PNPLA3) p.I148M variant and the female sex at the genetic and molecular level explains why premenopausal women remain protected against fatty liver disease (FLD) while a rapidly progressive disease ensues in…

CRC Upregulated 153-5p HIF-1 activates LINC00511, targeting HIF-1’s 3-UTR and +ve feedback loop [78] Oncogene Human CRC tissues vs. paired adjacent non-tumor and CRC cell lines HT29, LOVO, SW620, SW480 vs. normal colon epithelial FHC cell line and athymic BALB/c nude mice for implantation – HNF4 promotes LINC00511 transcription, interaction…

WDR6 is upregulated in response to insulin during insulin resistance We created an IR mouse model by feeding animals a HFD for 6 weeks20. Insulin injection was then performed (Fig. 1a). The phosphorylation of AKT, a key readout of the insulin signalling pathway21, showed decreased response to insulin, indicating resistance…

The real effect of CPSF3 on circRNA expression in HCC cells was tested by analyzing the total RNA fractions obtained from CPSF3-KO HepG2 cells, CPSF3-OE Bel7404 cells, and negative control cells using high-throughput sequencing (Supplementary Files S7 and S8). The data obtained showed that CPSF3-KO cells had more types and…

doi: 10.1186/s12864-023-09541-9. Affiliations Expand Affiliations 1 State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, 200031, China. shaozhenyu2017@sibcb.ac.cn. 2 Shanghai Key Laboratory of Medical Epigenetics, Institutes of Biomedical…

Each cell contains many mitochondria, and each mitochondria contains several copies of mtDNA [11]. mtDNA is located in the mitochondrial matrix in close proximity to the electron transport chain, the major source of reactive oxygen species. Therefore, mtDNA is particularly susceptible to oxidation leading to mutations and the development of…

Viral immune evasion is crucial to the pathogenesis of hepatitis B virus (HBV) infection. However, the role of HBV in the modulation of innate immune evasion is poorly understood. A liver-specific histone acetyltransferase 1 (Hat1) knockout (KO) mouse model and HAT1 KO cell line were established. Immunohistochemistry staining, Western blot…

1. Introduction The digestive tract greatly influences the maintenance of energy requirements in cattle due to its crucial role in digesting and absorbing nutrients. The rumen is an essential organ in the digestive tract for feed efficiency, with relatively high metabolic activities. The digestion of solid-feeding materials generates short-chain fatty…

The present study aims to investigate the impact of the gut microbiota and serum metabolites on the regulation of liver dysfunction in PCOS.PCOS rat models were established by treating Sprague Dawley (SD) rats with DHEA (an androgen, 60 mg/kg) and LET (a nonsteroidal aromatase inhibitor, 1 mg/kg) for 90 days. Hematoxylin and eosin…

Patients Tumour tissues from seven HBsAg-positive patients (6 men, 1 woman; mean age 66.7 ± 8 years) with HBV-related HCC and paired adjacent non-tumour tissues from six of them were studied. The clinical, histological, and virologic characteristics of the patients are summarised in Table 1. Two normal liver samples from HBV-negative patients who…

Objective: To investigate the effect of titanium dioxide nanoparticles (TiO2 NPs) on the expression profile of circular ribonucleic acid (circRNA) in human hepatocytes through in vitro cell experiments, and to attempt to understand the potential mechanism of hepatotoxicity through bioinformatics analysis. Methods: TiO2 NPs were characterized from the aspects of…

Key Points Liver metastasis, which is highly prevalent in advanced malignancies, was associated with poor prognosis. Contemporary therapies in liver metastasis were associated with the lack of metastatic-targeting ability, significant systemic toxicities and incapability of tumor microenvironment (TME) modulations. Lipid nanoparticles (LNP)-based strategies would overcome the deficiencies of conventional approaches…

Introduction Hepatic lipid accumulation and mitochondrial dysfunction are hallmarks of metabolic associated fatty liver disease (MAFLD), yet molecular parameters underlying MAFLD progression are not well understood. Differential methylation within the mitochondrial DNA (mtDNA) has been suggested to be associated with dysfunctional mitochondria, also during progression to Metabolic Steatohepatitis (MeSH). This…

Additional Information This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines. Optimized for Use With: CRISPRa (SAM) HeLa Cell Line (BPS Bioscience, #78193), CRISPRa (SAM) HepG2 Cell Line (BPS Bioscience, #78194) Article No BPS-78221 Country Availability SE, FI, DK, NO, IS, EE, LV,…

Bergmann, O. et al. Evidence for cardiomyocyte renewal in humans. Science 324, 98–102 (2009). Article  CAS  PubMed  PubMed Central  Google Scholar  Senyo, S. E. et al. Mammalian heart renewal by pre-existing cardiomyocytes. Nature 493, 433–436 (2013). Article  CAS  PubMed  Google Scholar  Bergmann, O. et al. Dynamics of cell generation and…

Mustacich, D. J., Bruno, R. S. & Traber, M. G. Vitamin E. Vitam. Horm. 76, 1–21 (2007). Article  CAS  PubMed  Google Scholar  Shahidi, F. & de Camargo, A. C. Tocopherols and tocotrienols in common and emerging dietary sources: Occurrence, applications, and health benefits. Int. J. Mol. Sci. 17, 1745 (2016)….

Preparation of GPMV GPMVs were prepared using different cell lines. Cells were cultured as follows: A549 (ATCC, Manassas, VA), HEK293 EBNA (herein referred to as HEK293, ATCC), HepG2 (ATCC), and Huh7 (ATCC) cells were kept in Dulbecco’s Modified Eagle’s Medium with high glucose (Sigma-Aldrich, Buchs, Switzerland) supplemented with 10% fetal…

Agency for Toxic Substances and Disease Registry (ATSDR) (2001) Toxicological profile for Benzidine. Update. Atlanta, GA: U.S. Department of Health and Human Services, Public Health Service. www.atsdr.cdc.gov/toxprofiles/tp62.pdf. Accessed 19 Jan 2023 Agnello M, Fontana M (2015) Survey on European studies of the chemical characterisation of tattoo ink products and the…

image: Figure 6: Overexpression of GPC1 increases proliferation of T24 cells. view more  Credit: 2023 Cheng et al. “This study was designed to increase the knowledge on the potential of GPCs and in particular GPC1 as a biomarker in cancer diagnosis and prognosis.”  BUFFALO, NY- March 22, 2023 – A new…

Overview Product name Description Rabbit polyclonal to Smad4 Host species Rabbit Tested applications Species reactivity Reacts with: HumanPredicted to work with: Mouse, Rat, Sheep, Horse, Cow, Dog, Pig, Chimpanzee,…

Overview Product name Description Rabbit monoclonal [SP306] to Smad4 – C-terminal Host species Rabbit Tested applications Species reactivity Reacts with: Mouse, Rat, Human Immunogen Synthetic…

The present study describes methodologies for culturing, freezing, thawing, processing, staining, labeling, and examining entire spheroids and organoids under various microscopes, while they remain intact in a hydrogel within a multipurpose device. Organoids and spheroids, being very fragile, get seriously damaged while handling. Our protocol ensures these 3D growing structures…

Overview Product name Description Rabbit polyclonal to Fbxw7 Host species Rabbit Tested applications Species reactivity Reacts with: Mouse, Rat, Human Immunogen Recombinant full length protein…

Overview Product name Description Rabbit monoclonal [EP618Y] to Smad4 Host species Rabbit Tested applications Suitable for: WB, IHC-Pmore detailsUnsuitable for: Flow Cyt,ICC/IF or IP Species reactivity Reacts with: Mouse,…

Worldwide, more than 300 million people are chronically infected with hepatitis B virus (HBV). Chronic HBV, an infection that lasts 6 months or longer, is not susceptible to hepatitis B vaccination. People living with chronic HBV are at high risk of liver cirrhosis or hepatocellular carcinoma, and in urgent need…

Here we provide a tool that directly selects biallelically edited knock-ins using cell-surface displays, thereby eliminating the need for extensive clonal verification. By doing so SNEAK PEEC greatly reduces the considerable effort currently expended to obtain biallelically edited clones. The ability to generate a clonal population with multiple edits is…

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Gebhardt, R. & Matz-Soja, M. Liver zonation: Novel aspects of its regulation and its impact on homeostasis. World J. Gastroenterol. 20, 8491–8504 (2014). PubMed  PubMed Central  Article  Google Scholar  Summar, M. L. et al. The incidence of urea cycle disorders. Mol. Genet Metab. 110, 179–180 (2013). CAS  PubMed  PubMed Central …

Clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) have transformed genome engineering techniques. Numerous toolsets have been created to enable easy and efficient loss-of-function perturbations of functional genomic sites. Study: CRISPR/Cas9 deletions induce adverse on-target genomic effects leading to functional DNA in human cells. Image Credit: elenabsl/Shutterstock The CRISPR/Cas9 system’s elements…

People with familial hypercholesterolemia, or FH, have very high levels of low-density lipoprotein cholesterol circulating in their blood due to aberrant LDL uptake by cells. With LDL levels elevated for prolonged periods, these patients are at increased risk for atherosclerotic cardiovascular disease. Transgelin is a protein that in humans is…

Showing result 1 – 5 of 34 swedish dissertations containing the word ChIP-Seq. Author : Ola Wallerman; Claes Wadelius; Jussi Taipale; Uppsala universitet; []Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; ChIP; ChIP-chip; ChIP-seq; transcription factors; motif discovery; nucleosome positioning; HepG2; genome-wide;…

Introduction Today, malignant tumors (cancer) still severely imperil human health and cause millions of global mortality rates.1,2 Deep-seated solid tumors are challenging to cure by most therapeutic tools, mainly blamed on the complex tumor microenvironment (TME).3,4 Adoptive cell therapy (ACT), as one of the effective immunotherapeutic means for cancer treatment,…