Tag: HepG2

Objective: To investigate the effect of titanium dioxide nanoparticles (TiO2 NPs) on the expression profile of circular ribonucleic acid (circRNA) in human hepatocytes through in vitro cell experiments, and to attempt to understand the potential mechanism of hepatotoxicity through bioinformatics analysis. Methods: TiO2 NPs were characterized from the aspects of…

Key Points Liver metastasis, which is highly prevalent in advanced malignancies, was associated with poor prognosis. Contemporary therapies in liver metastasis were associated with the lack of metastatic-targeting ability, significant systemic toxicities and incapability of tumor microenvironment (TME) modulations. Lipid nanoparticles (LNP)-based strategies would overcome the deficiencies of conventional approaches…

Introduction Hepatic lipid accumulation and mitochondrial dysfunction are hallmarks of metabolic associated fatty liver disease (MAFLD), yet molecular parameters underlying MAFLD progression are not well understood. Differential methylation within the mitochondrial DNA (mtDNA) has been suggested to be associated with dysfunctional mitochondria, also during progression to Metabolic Steatohepatitis (MeSH). This…

Additional Information This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines. Optimized for Use With: CRISPRa (SAM) HeLa Cell Line (BPS Bioscience, #78193), CRISPRa (SAM) HepG2 Cell Line (BPS Bioscience, #78194) Article No BPS-78221 Country Availability SE, FI, DK, NO, IS, EE, LV,…

Bergmann, O. et al. Evidence for cardiomyocyte renewal in humans. Science 324, 98–102 (2009). Article  CAS  PubMed  PubMed Central  Google Scholar  Senyo, S. E. et al. Mammalian heart renewal by pre-existing cardiomyocytes. Nature 493, 433–436 (2013). Article  CAS  PubMed  Google Scholar  Bergmann, O. et al. Dynamics of cell generation and…

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Preparation of GPMV GPMVs were prepared using different cell lines. Cells were cultured as follows: A549 (ATCC, Manassas, VA), HEK293 EBNA (herein referred to as HEK293, ATCC), HepG2 (ATCC), and Huh7 (ATCC) cells were kept in Dulbecco’s Modified Eagle’s Medium with high glucose (Sigma-Aldrich, Buchs, Switzerland) supplemented with 10% fetal…

Agency for Toxic Substances and Disease Registry (ATSDR) (2001) Toxicological profile for Benzidine. Update. Atlanta, GA: U.S. Department of Health and Human Services, Public Health Service. www.atsdr.cdc.gov/toxprofiles/tp62.pdf. Accessed 19 Jan 2023 Agnello M, Fontana M (2015) Survey on European studies of the chemical characterisation of tattoo ink products and the…

image: Figure 6: Overexpression of GPC1 increases proliferation of T24 cells. view more  Credit: 2023 Cheng et al. “This study was designed to increase the knowledge on the potential of GPCs and in particular GPC1 as a biomarker in cancer diagnosis and prognosis.”  BUFFALO, NY- March 22, 2023 – A new…

Overview Product name Description Rabbit polyclonal to Smad4 Host species Rabbit Tested applications Species reactivity Reacts with: HumanPredicted to work with: Mouse, Rat, Sheep, Horse, Cow, Dog, Pig, Chimpanzee,…

Overview Product name Description Rabbit monoclonal [SP306] to Smad4 – C-terminal Host species Rabbit Tested applications Species reactivity Reacts with: Mouse, Rat, Human Immunogen Synthetic…

The present study describes methodologies for culturing, freezing, thawing, processing, staining, labeling, and examining entire spheroids and organoids under various microscopes, while they remain intact in a hydrogel within a multipurpose device. Organoids and spheroids, being very fragile, get seriously damaged while handling. Our protocol ensures these 3D growing structures…

Overview Product name Description Rabbit polyclonal to Fbxw7 Host species Rabbit Tested applications Species reactivity Reacts with: Mouse, Rat, Human Immunogen Recombinant full length protein…

Overview Product name Description Rabbit monoclonal [EP618Y] to Smad4 Host species Rabbit Tested applications Suitable for: WB, IHC-Pmore detailsUnsuitable for: Flow Cyt,ICC/IF or IP Species reactivity Reacts with: Mouse,…

Worldwide, more than 300 million people are chronically infected with hepatitis B virus (HBV). Chronic HBV, an infection that lasts 6 months or longer, is not susceptible to hepatitis B vaccination. People living with chronic HBV are at high risk of liver cirrhosis or hepatocellular carcinoma, and in urgent need…

Here we provide a tool that directly selects biallelically edited knock-ins using cell-surface displays, thereby eliminating the need for extensive clonal verification. By doing so SNEAK PEEC greatly reduces the considerable effort currently expended to obtain biallelically edited clones. The ability to generate a clonal population with multiple edits is…

Malhi GS, Mann JJ. Depression. Lancet. 2018;392:2299–312. Article  Google Scholar  Wang B, Zhang H, Cao X. Research progress of non-monoamine antidepressants. Stress Brain. 2021;1:128–44. Article  Google Scholar  Coccaro EF. New hope for patients with major depressive disorder? N Engl J Med. 2019;381:980–1. Article  Google Scholar  Cai N, Chang S, Li…

Gebhardt, R. & Matz-Soja, M. Liver zonation: Novel aspects of its regulation and its impact on homeostasis. World J. Gastroenterol. 20, 8491–8504 (2014). PubMed  PubMed Central  Article  Google Scholar  Summar, M. L. et al. The incidence of urea cycle disorders. Mol. Genet Metab. 110, 179–180 (2013). CAS  PubMed  PubMed Central …

Clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) have transformed genome engineering techniques. Numerous toolsets have been created to enable easy and efficient loss-of-function perturbations of functional genomic sites. Study: CRISPR/Cas9 deletions induce adverse on-target genomic effects leading to functional DNA in human cells. Image Credit: elenabsl/Shutterstock The CRISPR/Cas9 system’s elements…

People with familial hypercholesterolemia, or FH, have very high levels of low-density lipoprotein cholesterol circulating in their blood due to aberrant LDL uptake by cells. With LDL levels elevated for prolonged periods, these patients are at increased risk for atherosclerotic cardiovascular disease. Transgelin is a protein that in humans is…

Showing result 1 – 5 of 34 swedish dissertations containing the word ChIP-Seq. Author : Ola Wallerman; Claes Wadelius; Jussi Taipale; Uppsala universitet; []Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; ChIP; ChIP-chip; ChIP-seq; transcription factors; motif discovery; nucleosome positioning; HepG2; genome-wide;…

Introduction Today, malignant tumors (cancer) still severely imperil human health and cause millions of global mortality rates.1,2 Deep-seated solid tumors are challenging to cure by most therapeutic tools, mainly blamed on the complex tumor microenvironment (TME).3,4 Adoptive cell therapy (ACT), as one of the effective immunotherapeutic means for cancer treatment,…